CN107402213A - Probe, probe apparatus and microscopic system - Google Patents
Probe, probe apparatus and microscopic system Download PDFInfo
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- CN107402213A CN107402213A CN201710760521.0A CN201710760521A CN107402213A CN 107402213 A CN107402213 A CN 107402213A CN 201710760521 A CN201710760521 A CN 201710760521A CN 107402213 A CN107402213 A CN 107402213A
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- probe
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- General Health & Medical Sciences (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The present invention provides a kind of probe, probe apparatus and microscopic system, wherein, the probe includes heat-absorbing block and passed as rod；The heat-absorbing block by inspection tissue to being cooled down, the illumination passage that insertion is set is offered on the heat-absorbing block, it is described to pass as one end of rod is stretched into the illumination passage, and expose from the heat-absorbing block with the surface being in contact by inspection tissue, it is described to pass as rod includes some optical elements for zooming into picture being arranged in order.The present invention can facilitate the position that virologist more directly selects observation to cut into slices, and it is lesions position to ensure final section, and can ensure the integrality of piece cutting structure.In addition, the present invention can reduce the quantity and virologist's workload of final stained slice, improve diagosis and go out the speed of report, while in turn ensure that the accuracy of pathological examination.
Description
Technical field
The present invention relates to technical field of medical equipment, more particularly to it is a kind of be used to carrying out section the probe of micro-imaging,
Probe apparatus and microscopic system.
Background technology
With clinical medical continuous development, pathology technique has turned into the important means for clinically differentiating at this stage, diagnosing,
Wherein, the clinical application range of quick frozen-section technology is wider, and application value is higher.The principle of quick frozen-section technology
For biological tissue is placed under cryogenic, and it is freezed.It is to be frozen to reach relevant criterion, then carry out at section
Reason, finally gives pathological diagnosis result.
For the paraffin section technology of routine, quick frozen-section technology is operationally more convenient and simple,
Clinically paid attention to by medical field.By using quick frozen-section technology, pathology department can receive specimens from pri
Clear and definite judgement is made to the disease event of patient in 15min, and notifies operative doctor to formulate clear and definite therapeutic scheme in time.
Frozen section needs that biological tissue is judged soundly and distinguished in a short time, therefore, quick Fabrication ice
It is the key for improving operating efficiency and ensureing diagnostic result accuracy to freeze section.In practical clinical, for specialty by
The frozen section expert of well trained is crossed, a slice, thin piece only needs a few minutes from cutting into slices to dyeing.If the however, place to tissue
Reason is very coarse, and said process may a few minutes to ten minutes.Such as running into bigger complicated tissue needs multiple staining, then
The time needed can be more, and can cause the increase of follow-up scoring time.
Therefore, in view of the above-mentioned problems, being necessary to propose further solution.
The content of the invention
It is used to carrying out section the probe of micro-imaging, probe apparatus and micro- it is an object of the invention to provide a kind of
System, to overcome the deficiencies in the prior art.
For achieving the above object, the present invention provides a kind of probe, and it is applied to be imaged by inspection tissue, the probe
Including heat-absorbing block and pass as rod；
The heat-absorbing block offers the illumination that insertion is set and led to being cooled down by inspection tissue on the heat-absorbing block
Road, the biography are stretched into the illumination passage as one end of rod, and are revealed from the heat-absorbing block with the surface being in contact by inspection tissue
Go out, it is described to pass as rod includes some optical elements for zooming into picture being arranged in order.
Wherein, the optical element is lens or light cone.
Wherein, it is described to pass as rod includes being arranged in order when the optical element is lens：First lens, the second lens,
3rd lens, the 3rd lens, the second lens and the first lens.
Wherein, in adjacent second lens and the 3rd lens, second lens are installed on the 3rd lens
On end face.
Wherein, it is described to pass as the outer surface of rod is provided with shading film plating layer.
Wherein, the illumination passage is opened in the central position of the heat-absorbing block.
For achieving the above object, the present invention provides a kind of probe apparatus, and it includes：Probe, pass as lighting tube and
Collecting unit；
The probe is probe as described above；The heat-absorbing block is positioned at the biography as one end of lighting tube, the illumination
Passage is passed as lighting tube is connected with described；It is described to pass as rod is contained in described pass as in lighting tube, the biography is another as rod
End leans with the collecting unit；The collecting unit includes imaging sensor, and the target surface of described image sensor forms institute
State the abutment face for passing the other end as rod.
Wherein, described image sensor is camera.
Wherein, the probe apparatus also includes circuit board, the circuit board and the phase mechatronics.
Wherein, the probe apparatus also includes symmetrically arranged two lamp guides, and any lamp guide is from the collection
Unit rises, and is passed by described as lighting tube, stretches into the illumination passage, and be in contact from the heat-absorbing block with by inspection tissue
Expose on surface.
Wherein, the lamp guide in the collecting unit bypasses described image sensor, and and described image sensor
Keep non-interference.
Wherein, the collecting unit also includes the shell of protection described image sensor, and the shell is antifreeze and heat conduction
The low shell of property.
For achieving the above object, the present invention provides a kind of microscopic system, and it includes：Probe apparatus, light supply apparatus, show
Showing device and control device；
The probe apparatus is probe apparatus as described above, the collecting unit and the control device data transfer；
The light supply apparatus provides the illumination needed for imaging for the probe apparatus, and the control system exports to the light supply apparatus
Illumination mode is controlled；The display device and the control system data transfer, and to the figure of control device transmission
As data are shown.
Wherein, the light supply apparatus includes some LED chips, and the light source output end of some LED chips is by described
The array output end of control system control.
Wherein, the probe apparatus also includes joint, the light source input line of the light supply apparatus and the number of control device
It is connected according to line with the joint.
Wherein, the control device includes main frame and control circuit, and the main frame is PC or FPGA or ASIC.
Wherein, the main frame also includes phase contrast processing module, and the phase contrast processing module generates phase contrast according to equation below
Image：
Wherein, I is final image；I1For the probe apparatus side lamp guide light after, the image of shooting, I2For institute
State after probe apparatus opposite side lamp guide lights, another image of shooting
Compared with prior art, the beneficial effects of the invention are as follows：The present invention can facilitate virologist more directly to select
The position of section is observed, it is lesions position to ensure final section, and can ensure the integrality of piece cutting structure.In addition, this hair
The bright quantity that can reduce final stained slice and virologist's workload, improve diagosis and go out the speed of report, while again
It ensure that the accuracy of pathological examination.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments described in invention, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of an embodiment of microscopic system of the present invention；
Fig. 2 is the enlarged diagram of probe apparatus in Fig. 1；
Fig. 3 is the biography in the present invention as the internal structure schematic diagram of an embodiment of rod.
Embodiment
The present invention is described in detail for shown each embodiment below in conjunction with the accompanying drawings, but it should explanation, these
Embodiment is not limitation of the present invention, those of ordinary skill in the art according to these embodiment institute work energy, method,
Or equivalent transformation or replacement in structure, belong within protection scope of the present invention.
As shown in figure 1, the present invention provides a kind of microscopic system by inspection tissue micro-imaging for being applied to extract from sick body,
Specifically, microscopic system of the invention includes：Probe apparatus 1, light supply apparatus 2, display device 3, control device 4 and sample stage
5。
The probe apparatus 1 is used for being positioned over being imaged by inspection tissue on sample stage 5, and the probe apparatus 1 is to connect
The probe apparatus 1 of touch.Wherein, the probe apparatus 1 includes：Probe 11, pass as lighting tube 12 and collecting unit 13.
As shown in Fig. 2 it is described probe 11 be used for by inspection tissue carry out freezing processing, and under conditions of illumination transmission by
Examine the image of tissue.Wherein, the probe 11 includes heat-absorbing block 111 and passed as rod 112.
When observation is by inspection tissue, the heat-absorbing block 111 has the surface being in contact with by inspection tissue, and in heat transfer
Effect is lower to be realized to being cooled by inspection tissue.In one embodiment, the heat-absorbing block 111 is shaped as flat circle
Cylindricality.It is further opened with axial direction penetrating the illumination passage of setting on the heat-absorbing block 111, the illumination passage is used for light source
The illumination provided in device 2 is mapped to by inspection tissue.Preferably, the illumination passage is opened in the center of the heat-absorbing block 111
Opening position.
It is described to pass as rod 112 is used to be in contact with by inspection tissue, and the image information by inspection tissue is transmitted to control system.
It is described to pass as one end of rod 112 is stretched into the illumination passage, and from the heat-absorbing block 111 with by examining the surface that is in contact of tissue
Expose.When the heat-absorbing block 111 by inspection tissue to being cooled down, it is described pass the Partial synchronization that spills as rod 112 with by
Inspection tissue is in contact, and realizes that contact zooms into picture.
As shown in figure 3, in order to realize it is described pass as the picture that zooms into of rod 112, it is described to pass as rod 112 includes some arranging successively
The optical element for zooming into picture of row.According to optical element quantity and the difference of arrangement, the biography can be controlled as rod 112 has
There is the enlargement ratio of demand.
Wherein, the optical element can be lens or light cone.In the embodiment that the optical element is lens,
The lens include：First lens 1121, the second lens 1122, the 3rd lens 1123.Now, each lens are saturating according to first
Mirror 1121, the second lens 1122, the 3rd lens 1123, the 3rd lens 1123, the second lens 1122 and the first lens 1121
Mode is arranged in order.
Specifically, there is the spacing of light conduction between first lens 1121 and second lens 1122, described the
There is the spacing of light conduction between three lens 1123.In order to realize that light is transferred to exit end from the first lens 1121 of incidence end
First lens 1121, the curved surface that the side end face of the first lens 1,121 1 is set for depression, another side end face are what evagination was set
Curved surface；The side end face of second lens 1,122 1 is the curved surface that evagination is set, and another side end face is the curved surface that depression is set；It is described
The side end face of 3rd lens 1,123 1 is the curved surface that evagination is set, and another side end face is plane.
In adjacent the first lens 1121 and the second lens 1122, end face that the evaginations of first lens 1121 is set with
The curved surface that the evagination of second lens 1122 is set is oppositely arranged.In the second adjacent lens 1122 and the 3rd lens 1123,
On the end face that the evagination that the curved surface that the depression of second lens 1122 is set is installed on the 3rd lens 1123 is set.Adjacent
In 3rd lens 1123, the planar end of the 3rd lens 1123 is oppositely arranged.
So as to, it is described to pass as rod 112 is designed according to centre wavelength for 510nm in above-mentioned embodiment, by 6 thoroughly
Microscope group into biography as rod 112,1mm*1mm field range, and 1.4um resolution ratio, 1 times of magnifying power can be reached.
In addition, in order to prevent veiling glare or exciting light from entering microscopic system, result is disturbed, the biography is as rod 112
Outer surface be additionally provided with shading film plating layer.
The biography is as lighting tube 12 is between the collecting unit 13 and heat-absorbing block 111.Wherein, the heat-absorbing block 111
Passed positioned at described as one end of lighting tube 12, the illumination passage are passed as lighting tube 12 is connected with described.Meanwhile the biography picture
Rod 112 be contained in it is described pass as in lighting tube 12, and keep described and pass as rod 112 and pass certain as having between lighting tube 12
Gap.Preferably, it is described to pass as rod 112 is with passing as lighting tube 12 keeps being coaxially disposed.
The collecting unit 13 is used to receive the image information for passing and transmitting as rod 112, and is fed back to control dress
Put 4.The collecting unit 13 includes imaging sensor 131 and the shell 130 protected to described image sensor 131,
Wherein, the shell 130 uses antifreeze and low thermal conductivity shell.The target surface of described image sensor 131 forms the biography picture
The abutment face of the other end of rod 112.So as to, described image sensor 131 target surface directly with passing as rod 112 is in contact, so
The collecting unit 13 is directly detected as the end face of rod 112 to passing, and saves lens imaging step, and final resolution ratio is sensed
Device pixel determines.
In one embodiment, described image sensor 131 is camera, and the camera, which has, receives the biography as rod 112
The target surface 1311 by inspection organization chart picture information transmitted.Meanwhile the collecting unit 13 also includes being controlled the camera
Circuit board 132, the circuit board 132 and the phase mechatronics.Wherein, the camera and circuit board 132 have hot plug
The data wire and supply lines of formula, it can so ensure that probe departs from wire rod.
In above-mentioned embodiment, the camera is specifically as follows the camera lens module of smart mobile phone.By using the mirror of mobile phone
Head mould group so that collecting unit 13 has high resolution (pixel is small), and cheap, target surface size is larger, can cover big chi
Very little biography forms big visual field as rod 112, the advantages that high-resolution imaging effect.
Further, in order to realize the transmission of data between the collecting unit 13 and control device 4, the probe apparatus
1 also includes joint 14, and the data wire 41 of the control device 4 and the data wire of the camera are connected on the joint 14.
In addition, the probe apparatus 1 also includes symmetrically arranged two lamp guides 15, above-mentioned two lamp guides 15 are used for real
The phase contrast formula imaging of the existing present invention.Specifically, any lamp guide 15 is passed as shining from the collecting unit 13 by described
Gap in bright pipe 12, stretch into the illumination passage, and expose from the heat-absorbing block 111 with the surface being in contact of cutting into slices.For
The lamp guide 15 is avoided to cause to interfere to imaging sensor 131, the lamp guide 15 in the collecting unit 13 bypasses
Described image sensor 131, and keep non-interference with described image sensor 131.
The light supply apparatus 2 is used to provide the illumination needed for imaging for the probe apparatus 1.Specifically, the light source dress
Putting 2 includes some LED chips, and the light source output end of some LED chips is that the array controlled by the control system exports
End.So as to which different lighting systems can be selected by the control device 4, and then can be carried out into according to different modes
Picture.The array output end is connected by light source input line 21 with the joint 14 of the probe apparatus 1, and the lamp guide 15 can
It is coupled with as needed with the light source input line 21.
The control device 4 is used to receive the image information that the collecting unit 13 gathers, and it is calculated and divided
Analysis.Specifically, the control device 4 includes main frame and control circuit, wherein, the main frame both can be high performance PC,
Can be the circuit boards such as FPGA or ASIC.Meanwhile the control device 4 is also carried out to the collecting unit 13 and light supply apparatus 2
Control.
In addition, in order to realize that phase contrast formula is imaged, the main frame also includes phase contrast processing module, and the phase contrast processing module is pressed
Phase contrast image is generated according to equation below：
Wherein, I is final image；I1For the probe apparatus side lamp guide light after, the image of shooting, I2For institute
State after probe apparatus opposite side lamp guide lights, another image of shooting.
And I in formula (A)1-I2And I1+I2Represent that each pixel in two images is strong and weak mutually to be added and subtracted, such as image
I1For 256*256 picture element matrix, I2Also it is 256*256 picture element matrix, then I1-I2Represent coordinate identical in two images
The luminous intensity of pixel is subtracted each other, I1+I2Then represent that the luminous intensity of coordinate identical pixel in two images is added, and in the present invention
I1And I2The pixel of two images is exactly like, therefore obtains disparate images I with formula (A) come computing.
The display device 3 is used to show main frame output image progress high-resolution.Specifically, the display device 3
Including display, the display and the control system data transfer, and the view data of the control device 4 transmission is entered
Row display.
Microscopic system based on constituted above, the present invention have autofluorescence microscope modes and dye-free differential phase contrast micro-
Pattern.
When needing select autofluorescence microscope modes, sample is completed after cutting into slices several times on slicer, obtains table
Face is smooth by inspection tissue.It is attached to popping one's head on the surface by inspection tissue, heat-absorbing block is popped one's head in being cooled down by inspection tissue
Middle position is observed.Now, the exciting light required for autofluorescence, example are sent by control device control light supply apparatus
As, it is necessary to observe FAD autofluorescence, then exciting light uses 445nm blue light.So as to have filter action using to 445nm
Pass as rod, it is observed that in sample FAD autofluorescence.
The advantages of above-mentioned autofluorescence microscope modes, is that autofluorescence provides the distributed image of specific molecular in tissue, compares
The autofluorescence in normal temperature situation, the autofluorescence of freezing microtome section have the characteristics of quantum yield is high, generally 10 under a normal temperature times left side
The right side, so as to which the requirement to camera substantially reduces, accelerate picking rate.And autofluorescence is Intrinsic fluorescence characteristic, Ke Yiyong
To evaluate the energetic supersession state of cell.For example, NADH and FAD indicate respectively the state of oxidation and reducing condition of cell, wherein,
Intensity rate is defined as redox ratio, the energetic supersession state of indicator cells.
When need carry out select dye-free differential phase contrast microscope modes when, now, phase contrast imaging pattern need not observe by
The fluorescence being excited in inspection tissue., it is necessary to be lighted successively using two lamp guides during phase contrast imaging, i.e. side lamp guide lights
Afterwards, an image I is shot1, then opposite side lamp guide light, shoot another image I2.By two images according to equation below
Carry out phase contrast processing：The generation of phase contrast image can be completed.
The advantages of above-mentioned dye-free differential phase contrast microscope modes, is, can provide the structure distribution information of sample, due to
Differential phase contrast pattern can provide a kind of plastic effect, so as to show small deformation.Phase contrast by inspection tissue to being reflected
Rate is sensitive, and tissue or cell boundaries can be highlighted effectively.
In addition, importantly, above two imaging pattern is all very low to time requirement, can be with real time imagery, cold
But complete to detect while by inspection tissue, auxiliary doctor judges to change whether position is worth dyeing.Therefore, once auxiliary doctor finds
Pathological tissues just complete the work of frozen section, improve operating efficiency, shorten the stand-by period of clinician, alleviate
The pain of patient.
In summary, the present invention can facilitate the position that virologist more directly selects observation to cut into slices, and ensure final
Section is lesions position, and can ensure the integrality of piece cutting structure.In addition, the present invention can reduce the number of final stained slice
Amount and virologist's workload, improve diagosis and go out the speed of report, while in turn ensure that the accuracy of pathological examination.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (17)
1. one kind probe, it is applied to be imaged by inspection tissue, it is characterised in that the probe includes heat-absorbing block and passed as rod；
The heat-absorbing block offers the illumination passage that insertion is set, institute to being cooled down by inspection tissue on the heat-absorbing block
Biography is stated as one end of rod is stretched into the illumination passage, and is exposed from the heat-absorbing block with by the surface that is in contact of tissue is examined, institute
Biography is stated as rod includes some optical elements for zooming into picture being arranged in order.
2. probe according to claim 1, it is characterised in that the optical element is lens or light cone.
3. probe according to claim 1, it is characterised in that described to pass as rod includes when the optical element is lens
It is arranged in order：First lens, the second lens, the 3rd lens, the 3rd lens, the second lens and the first lens.
4. probe according to claim 3, it is characterised in that described in adjacent second lens and the 3rd lens
Second lens are installed on the end face of the 3rd lens.
5. probe according to claim 1, it is characterised in that described to pass as the outer surface of rod is provided with shading film plating layer.
6. probe according to claim 1, it is characterised in that the illumination passage is opened in the central position of the heat-absorbing block
Put place.
7. a kind of probe apparatus, it is characterised in that the probe apparatus includes：Probe, pass as lighting tube and collecting unit；
The probe is the probe as described in any one of claim 1 to 6；The heat-absorbing block is positioned at the biography as the one of lighting tube
End, the illumination passage are passed as lighting tube is connected with described；It is described pass as rod be contained in it is described pass as in lighting tube, the biography
As the other end of rod leans with the collecting unit；The collecting unit includes imaging sensor, described image sensor
Target surface forms the abutment face for passing the other end as rod.
8. probe apparatus according to claim 7, it is characterised in that described image sensor is camera.
9. probe apparatus according to claim 8, it is characterised in that the probe apparatus also includes circuit board, the electricity
Road plate and the phase mechatronics.
10. probe apparatus according to claim 7, it is characterised in that the probe apparatus also includes symmetrically arranged two
Root lamp guide, any lamp guide, by the biography as lighting tube, stretch into the illumination passage from the collecting unit
In, and expose from the heat-absorbing block with the surface being in contact by inspection tissue.
11. probe apparatus according to claim 7, it is characterised in that the lamp guide in the collecting unit bypasses
Described image sensor, and keep non-interference with described image sensor.
12. probe apparatus according to claim 7, it is characterised in that the collecting unit also includes protection described image
The shell of sensor, the shell are antifreeze and low thermal conductivity shell.
13. a kind of microscopic system, it is characterised in that the microscopic system includes：Probe apparatus, light supply apparatus, display device with
And control device；
The probe apparatus is the probe apparatus as described in any one of claim 7 to 12, the collecting unit and the control
Device data transfer；The light supply apparatus provides the illumination needed for imaging for the probe apparatus, and the control system is to described
The illumination mode of light supply apparatus output is controlled；The display device and the control system data transfer, and to the control
The view data of device transmission processed is shown.
14. microscopic system according to claim 13, it is characterised in that the light supply apparatus includes some LED chips, institute
The light source output end for stating some LED chips is the array output end controlled by the control system.
15. microscopic system according to claim 13, it is characterised in that the probe apparatus also includes joint, the light
The light source input line of source device and the data wire of control device are connected with the joint.
16. microscopic system according to claim 13, it is characterised in that the control device includes main frame and control electricity
Road, the main frame are PC or FPGA or ASIC.
17. microscopic system according to claim 16, it is characterised in that the main frame also includes phase contrast processing module, institute
State phase contrast processing module and generate phase contrast image according to equation below：
<mrow>
<mi>I</mi>
<mo>=</mo>
<mfrac>
<mrow>
<msub>
<mi>I</mi>
<mn>1</mn>
</msub>
<mo>-</mo>
<msub>
<mi>I</mi>
<mn>2</mn>
</msub>
</mrow>
<mrow>
<msub>
<mi>I</mi>
<mn>1</mn>
</msub>
<mo>+</mo>
<msub>
<mi>I</mi>
<mn>2</mn>
</msub>
</mrow>
</mfrac>
<mo>;</mo>
</mrow>
Wherein, I is final image；I1For the probe apparatus side lamp guide light after, the image of shooting, I2For the spy
After head device opposite side lamp guide lights, another image of shooting.
Priority Applications (1)
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| CN201710760521.0A CN107402213B (en) | 2017-08-29 | 2017-08-29 | Probe, probe device and microscope system |
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| CN201710760521.0A CN107402213B (en) | 2017-08-29 | 2017-08-29 | Probe, probe device and microscope system |
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| US6413252B1 (en) * | 1998-02-26 | 2002-07-02 | Lucid, Inc. | Confocal microscope for facilitating cryosurgery of tissue |
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| US20020115908A1 (en) * | 2000-06-30 | 2002-08-22 | Inner Vision Imaging, L.L.C. | Endoscope |
| US20060155267A1 (en) * | 2005-01-10 | 2006-07-13 | Nir Berzak | Thermal mapping of a cryoablation volume, for image-guided cryosurgery |
| WO2007123684A2 (en) * | 2006-04-04 | 2007-11-01 | Biotime, Inc. | Methods and devices for thawing and/or imaging frozen biological samples |
| US20080033455A1 (en) * | 2006-08-03 | 2008-02-07 | Rassman William R | Hair extraction device and method for its use |
| CN103975229A (en) * | 2011-09-29 | 2014-08-06 | 迈阿密大学 | Ultra-rapid diagnostic tissue preparation as an alternative to frozen section |
| CN104765138A (en) * | 2015-04-17 | 2015-07-08 | 南京理工大学 | Multi-mode micro-imaging system and method based on LED array |
| WO2017012555A1 (en) * | 2015-07-20 | 2017-01-26 | 徐敏 | Photon structure and chemometrics pathologic system |
| US20170027503A1 (en) * | 2015-07-31 | 2017-02-02 | University Of Utah Research Foundation | Devices, systems, and methods for imaging and treating a selected tissue |
| CN207351910U (en) * | 2017-08-29 | 2018-05-11 | 苏州精观医疗科技有限公司 | Probe, probe apparatus and microscopic system |
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