CN107396922A - A kind of coating agent for promoting verbena hybrida seed to sprout and its application method - Google Patents
A kind of coating agent for promoting verbena hybrida seed to sprout and its application method Download PDFInfo
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- CN107396922A CN107396922A CN201710492540.XA CN201710492540A CN107396922A CN 107396922 A CN107396922 A CN 107396922A CN 201710492540 A CN201710492540 A CN 201710492540A CN 107396922 A CN107396922 A CN 107396922A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
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- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of coating agent for promoting verbena hybrida seed to sprout and its application method, belong to coating agent field.The verbena hybrida coating agent for seed, is made up of film forming agent, water-loss reducer, plant growth regulator and nano titanium oxide, and the concentration of each component is：0.1~0.4g/L of film forming agent, 0.2~0.3g/L of water-loss reducer, 0.2~0.4g/L of plant growth regulator, 10~30g/L of nano titanium oxide.For the present invention by the use of verbena hybrida coating agent for seed active ingredient, the germinating time for substantially reducing seed, the germination percentage for improving seed and pollution rate are low, and cost is cheap, easy to operate, and technical support is provided for verbena hybrida seed pelleting.
Description
Technical field
The invention belongs to coating agent field, and in particular to a kind of coating agent for promoting verbena hybrida seed to sprout and its user
Method.
Background technology
Verbena hybrida is Verenaceae, Verbena herbaceos perennial, also known as careless Lantana camara, PUDIJIN, Mei Renying.It is former
Are produced from the ground such as Brazil, Peru, Uruguay, now cultivation extensively all over the world, its plant is short strong, and plant type is attractive in appearance, and pattern is bright-coloured various, has
In vain, it is red, blue, lilac, pink etc. tool fragrance, the florescence is the 5-11 months, and the florescence is longer, and property is very strong, and sunlight in the family way, non-shade tolerant,
The advantages that compared with cold-resistant, Salt And Alkali Tolerance, impoverishment tolerant soil, extensive management, low, quick, mass function is strong cost, verbena hybrida turns into one
Kind rare garden landscape vegetable material, available for pergola on the city road, traffic cycle, hillside fields, flower bed, flower border etc.,
Can make potted flower into large area transplant in be adapted to it is potted plant view and admire or arrange in flower stand garden woods unoccupied place, tree altar, for solution southern winter
And the Northeast's showy flowers of herbaceous plants is of less types, especially at the end of spring and the beginning of summer lacks the problem of spending and a kind of good plant material, Er Qieyou are provided
The symbol implied meaning of " mutually keeping, family harmony ", therefore, liked by domestic increasing consumer, its is potted plant or hang potted plant
Kind, for decorating household, also life can be allowed to be filled with sentiment with fresh air, the breath of natural harmony is brought for whole family.
Meanwhile herb can also be used as medicine, and have the effect of clearing heat and cooling blood.
Verbena hybrida percentage of seedgermination is low, germination is slow, just starts to emerge through 2~3 weeks.At present, mainly by conventional method kind
Support and manage, germination percentage is low, germination is slow, adds the number reseeded repeatedly, and causes verbena hybrida plant lazy weight, it is difficult to
Meet the needs of consumers in general, cause verbena hybrida economic and social benefit undesirable, leverage it and commercially produce.
The research about verbena hybrida is concentrated mainly on landscape utilization, cultivation technique and prevention and control of plant diseases, pest control etc. in recent years, only few
Amount document is sprouted to verbena hybrida seed to be conducted a preliminary study, and there is not been reported for the research to verbena hybrida seed pelleting.
The essence of seed coating technology is exactly to utilize sticker or film forming agent, and bactericide, insecticide, micro- fertilizer, plant are given birth to
The non-seed material such as long conditioning agent, colouring agent or filler, it is wrapped in outside seed, to reach seed globulate or basic guarantor
Original shape is held, improves resistance, disease resistance, accelerates germination, promotes seedling, increases yield, improves a seed skill of quality
Art.Therefore, the research for promoting verbena hybrida seed to sprout has become verbena hybrida seed coating technology development urgent problem to be solved.
The content of the invention
It is an object of the invention to provide a kind of coating agent for promoting verbena hybrida seed to sprout and its application method, the coating
Verbena hybrida capsuled seed sprout time is short made of agent, germination percentage is high, pollution rate is low, and cost is cheap, easy to operate, is beauty
Cherry seed pelleting provides technical support, and foundation is provided for the large-scale production and Landscape Application of verbena hybrida.
The present invention use concrete technical scheme for：
A kind of coating agent for promoting verbena hybrida seed to sprout, by film forming agent, water-loss reducer, plant growth regulator and nanometer two
Titanium oxide forms, the concentration of each component：Film forming agent is 0.1~0.4g/L, and water-loss reducer is 0.2~0.3g/L, plant growth regulating
Agent is 0.2~0.4g/L, and nano titanium oxide is 10~30g/L.
Further, the film forming agent by one or more mixing in chitosan, sodium carboxymethylcellulose, sodium alginate and
Into the water-loss reducer is polyacrylic acid potassium, and the plant growth regulator is by methyl α-naphthyl acetate, 6-benzyl aminopurine, gibberellin
One or more mix, and the nano titanium oxide is nanometer anatase titania, and its particle diameter is 15 ± 5nm.
Further, the concentration of the chitosan is 0.3g/L, and the concentration of polyacrylic acid potassium is 0.25g/L, 6- benzyl amino
The concentration of purine is 0.3g/L, dichloride in anatase type TiO2Concentration be 30g/L.
A kind of application method for the coating agent for promoting verbena hybrida seed to sprout, comprises the following steps：
Step 1), verbena hybrida seed are pre-processed
Select it is full, without seed that is damaged, not carrying disease pest ovum, clean standby, then carry out seed disinfection；
Step 2), the verbena hybrida seed by pretreatment is placed in coating agent and is coated
Verbena hybrida seed by pretreatment is placed in coating agent, is then 1 in the ratio of seed and coating agent:30 enter
Row coating, is well mixed, and then puts in an oscillator uniformly vibration 2-3h, verbena hybrida capsuled seed is made, will finally be coated
Seed natural air drying, experiment culture it is standby.
Further, it is specially to seed disinfection in the step 1)：The seed selected is placed in 75% alcoholic solution
Soak and vibrate 2-6min, aseptic water washing 3-5 times, then be placed in 0.1% mercuric chloride solution and soak and vibrate 3-8min, it is sterile
Water rinses 3-6 times.
Beneficial effects of the present invention：
The verbena hybrida coating agent for seed of the present invention, 6-benzyl aminopurine and nanometer anatase titania, which have, promotes seed
The characteristics of sprouting, have the function that to bond using chitosan and promote seed to sprout, utilize the strong water absorbing capacity of polyacrylic acid potassium
With strong water retaining function, it is set to keep the moisture needed for germination under badly dry natural conditions, to ensure verbena hybrida kind
The germination percentage of son, and the proportioning of each component of coating agent is in optimized selection, make its coating effect best.Therefore, use
After verbena hybrida coating agent for seed of the present invention is to verbena hybrida seed treatment, substantially reduce seed germinating time, significantly
Improve the germination percentage of seed and pollution rate is low, so as to meet industrialized large-scale plantation, reduce the number reseeded repeatedly, drop
Low planting cost, while nanometer anatase titania has antibacterial action so that verbena hybrida capsuled seed is easy to maintain, is not easy to send out
It is mould.
Embodiment
Technical scheme is described in further details with reference to embodiment.
In embodiments of the invention, a kind of coating agent for promoting verbena hybrida seed to sprout, by film forming agent, water-loss reducer, plant
Growth regulator and nano titanium oxide composition, the concentration of each component：Chitosan is 0.1~0.4g/L, polyacrylic acid potassium 0.2
~0.3g/L, 6-benzyl aminopurine are 0.2~0.4g/L, nanometer anatase titania (particle diameter is 15 ± 5nm) is 10~
30g/L。
The specifically used method of the coating agent is：
Step A, verbena hybrida Seeds preprocess：
Select first it is full, without verbena hybrida seed that is damaged, not carrying disease pest ovum, it is 10g's then to weigh 10 parts of quality
Seed, it is put in respectively in beaker, rinses 20min with running water, clean standby；Clean standby seed is placed in 75% wine again
Soaked in smart solution and vibrate 3min, aseptic water washing 3 times, be subsequently placed in and 4min is soaked and vibrated in 0.1% mercuric chloride solution,
Aseptic water washing 5 times；
Step B, verbena hybrida seed pelleting：
The pretreated seeds of step A are placed in coating agent, are 1 in the ratio of seed and coating agent:30 are coated,
It is well mixed, uniformly vibration 2h is then put in an oscillator, verbena hybrida capsuled seed is made, is finally placed on the seed being coated
Natural air drying on gauze, experiment culture are standby.Standby verbena hybrida capsuled seed will be tested, be arranged in tweezers are neat containing two
Layer is sufficiently humidified so as in the glass culture dish of filter paper, and these apparatus pass through high-pressure sterilizing pot high-temperature sterilization, to avoid miscellaneous bacteria from infecting
Seed, each culture dish place 50 seeds, and setting repeats, covers culture dish lid and be put in constant temperature illumination box three times
Germination test is carried out, constant incubator temperature is 20 DEG C, and constant incubator humidity is 75~85%, at daily illumination 16h, dark
8h is managed, incubation time is 25 days.During this period, every morning 9:00 observation, record verbena hybrida seed sprouts and pollution condition, and
Culture dish wetting is kept, quantitatively adds 2mL distilled water, verbena hybrida seedling length, root long are measured at the end of culture.
During seed pelleting, the selection of film forming agent is a crucial step, if film forming agent selection is improper, it is impossible to right
Seed Development effectively wraps up.Bad film forming agent can cause the generations such as seed parcel is uneven, and film easily comes off.Therefore, it is
The film forming agent of screening optium concentration, chitosan solution is arranged to 0.1,0.2,0.3,0.4g/L tetra- gradients, respectively to U.S.
Female's cherry seed is coated and sprouted experiment, 4 groups of progress of experiment point, separately adds one group of blank control, the results are shown in Table shown in 1.
The influence that the Chitosan Coating of table 1 is sprouted to verbena hybrida seed
| Experimental group | Germination rate | Pollution rate | Sprout time |
| ck | 8.42% | 97.65% | 10d |
| 0.1g/L | 30.93% | 71.76% | 7d |
| 0.2g/L | 41.66% | 54.44% | 5d |
| 0.3g/L | 57.23% | 28.35% | 3d |
| 0.4g/L | 29.54% | 27.38% | 3d |
From table 1 it follows that the chitosan of various concentrations is coated to verbena hybrida seed, compared with control group, all
The germination rate and speed of germinating of verbena hybrida seed can be improved, and with the raising of chitosan concentration, the germination rate of verbena hybrida seed
The trend for first raising and reducing afterwards is presented, when chitosan is 0.3g/L, germination rate reaches 57.23%, and sprout time is most short, dirty
Dye rate is minimum.
In the case where film forming agent is optimal, in order to screen the optimal coating agent of verbena hybrida seed, repeat step B, design is just
Experiment is handed over, 9 groups of experiments is made altogether and numbers, separately adds one group of blank control, in 9 groups of experiments, verbena hybrida seed chitosan is
0.3g/L, 6-benzyl aminopurine content (A), polyacrylic acid potassium content (B) and nanometer anatase titania (TiO2) content (C)
The design of three factors, factor level are shown in Table 2, and Orthogonal experiment results are shown in Table 3.
The 6-benzyl aminopurine of table 2, polyacrylic acid potassium and dichloride in anatase type TiO2The L of three factors9(33) orthogonal design
The Orthogonal experiment results of table 3
| Experimental group | A | B | C | Germination rate | Pollution rate | Sprout time |
| ck | 0 | 0 | 0 | 7.65% | 98.73% | 21d |
| 1 | 1 | 1 | 1 | 51.34% | 68.34% | 11d |
| 2 | 1 | 2 | 2 | 83.40% | 63.40% | 9d |
| 3 | 1 | 3 | 3 | 76.65% | 26.65% | 6d |
| 4 | 2 | 1 | 2 | 87.54% | 37.54% | 6d |
| 5 | 2 | 2 | 3 | 95.76% | 12.76% | 1d |
| 6 | 2 | 3 | 1 | 91.55% | 21.55% | 3d |
| 7 | 3 | 1 | 3 | 87.42% | 17.42% | 4d |
| 8 | 3 | 2 | 1 | 83.83% | 53.83% | 3d |
| 9 | 3 | 3 | 2 | 78.67% | 18.67% | 2d |
From table 3 it is observed that in the case where chitosan is 0.3g/L level, the 6-benzyl aminopurine of different content, poly- third
Olefin(e) acid potassium and dichloride in anatase type TiO2Combination is coated to verbena hybrida seed, can significantly improve the germination rate of verbena hybrida seed
And speed of germinating；All in all, the 5th and 6 group of germination rate highest, 95.76% and 91.55%, and the 5th group of sprouting are respectively reached
Time is most short, and pollution rate is minimum.Illustrate 6-benzyl aminopurine 0.3g/L in the present invention, polyacrylic acid potassium 0.25g/L, nano-silica
The combination for changing titanium 30g/L is that germination rate highest, sprout time are most short, the minimum optimal coating agent of pollution rate.
A kind of coating agent for promoting verbena hybrida seed to sprout provided by the present invention and preparation method thereof is carried out above
Be discussed in detail, apply herein specific case to the present invention principle and embodiment be set forth, be noted that with
The upper only presently preferred embodiments of the present invention, is not intended to limit the invention.It is all the spirit and principles in the present invention it
Interior made all any modification, equivalent and improvement etc., should be included in the scope of the protection.
Claims (9)
1. a kind of coating agent for promoting verbena hybrida seed to sprout, it is characterised in that by film forming agent, water-loss reducer, plant growth regulating
Agent and nano titanium oxide composition, the concentration of each component：Film forming agent is 0.1~0.4g/L, and water-loss reducer is 0.2~0.3g/L, is planted
Thing growth regulator is 0.2~0.4g/L, and nano titanium oxide is 10~30g/L.
A kind of 2. coating agent for promoting verbena hybrida seed to sprout according to claim 1, it is characterised in that the film forming agent
Mixed by the one or more in chitosan, sodium carboxymethylcellulose, sodium alginate.
A kind of 3. coating agent for promoting verbena hybrida seed to sprout according to claim 1, it is characterised in that the water-loss reducer
For polyacrylic acid potassium.
A kind of 4. coating agent for promoting verbena hybrida seed to sprout according to claim 1, it is characterised in that the plant life
Long conditioning agent is mixed by the one or more in methyl α-naphthyl acetate, 6-benzyl aminopurine, gibberellin.
A kind of 5. coating agent for promoting verbena hybrida seed to sprout according to claim 1, it is characterised in that the nanometer two
Titanium oxide is nanometer anatase titania.
A kind of 6. coating agent for promoting verbena hybrida seed to sprout according to claim 5, it is characterised in that the anatase titanium dioxide
Nano-TiO2Particle diameter be 15 ± 5nm.
7. the coating agent that a kind of promotion verbena hybrida seed according to claim 1-5 is sprouted, it is characterised in that the shell gathers
The concentration of sugar is 0.3g/L, and the concentration of polyacrylic acid potassium is 0.25g/L, and the concentration of 6-benzyl aminopurine is 0.3g/L, anatase titanium dioxide
Nano-TiO2Concentration be 30g/L.
8. a kind of application method for the coating agent for promoting verbena hybrida seed to sprout, it is characterised in that comprise the following steps：
Step 1), verbena hybrida seed are pre-processed
Select it is full, without seed that is damaged, not carrying disease pest ovum, clean standby, then carry out seed disinfection；
Step 2), the verbena hybrida seed by pretreatment is placed in coating agent and is coated
Verbena hybrida seed by pretreatment is placed in coating agent, is then 1 in the ratio of seed and coating agent:30 are wrapped
Clothing, it is well mixed, then puts in an oscillator uniformly vibration 2-3h, verbena hybrida capsuled seed is made, the kind being finally coated
Sub- natural air drying, experiment culture are standby.
A kind of 9. application method of coating agent for promoting verbena hybrida seed to sprout according to claim 8, it is characterised in that
To seed disinfection in the step 1), it is specially：The seed selected is placed in 75% alcoholic solution and soaks and vibrate 2-
6min, aseptic water washing 3-5 times, then be placed in 0.1% mercuric chloride solution and soak and vibrate 3-8min, aseptic water washing 3-6 times.
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Cited By (5)
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| CN111727689A (en) * | 2020-07-16 | 2020-10-02 | 榆林市农业科学研究院 | High-temperature sterilization method for pea seeds |
| CN114988946A (en) * | 2022-06-02 | 2022-09-02 | 安徽农业大学 | High-temperature-resistant coating agent for non-heading Chinese cabbage seeds and preparation and use methods thereof |
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| CN108633383A (en) * | 2018-07-09 | 2018-10-12 | 贵州大学 | A kind of nanometer of coat pelleted seeds and preparation method thereof |
| CN108633383B (en) * | 2018-07-09 | 2021-05-28 | 贵州大学 | Nano-coated pelleted seed and preparation method thereof |
| CN110754483A (en) * | 2019-10-29 | 2020-02-07 | 贵州大学 | Pinus massoniana seed coating agent and preparation method and application thereof |
| CN110754483B (en) * | 2019-10-29 | 2021-04-09 | 贵州大学 | Pinus massoniana seed coating agent and preparation method and application thereof |
| CN111727688A (en) * | 2020-07-16 | 2020-10-02 | 榆林市农业科学研究院 | High-temperature sterilization method for mung bean seeds |
| CN111727689A (en) * | 2020-07-16 | 2020-10-02 | 榆林市农业科学研究院 | High-temperature sterilization method for pea seeds |
| CN114988946A (en) * | 2022-06-02 | 2022-09-02 | 安徽农业大学 | High-temperature-resistant coating agent for non-heading Chinese cabbage seeds and preparation and use methods thereof |
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