CN107384772A - One kind can scale paper substrate detection chip and its manufacture method - Google Patents
One kind can scale paper substrate detection chip and its manufacture method Download PDFInfo
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- CN107384772A CN107384772A CN201710760443.4A CN201710760443A CN107384772A CN 107384772 A CN107384772 A CN 107384772A CN 201710760443 A CN201710760443 A CN 201710760443A CN 107384772 A CN107384772 A CN 107384772A
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- paper substrate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/30—2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid), i.e. ABTS
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
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- Emergency Medicine (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention mainly relates to one kind can scale paper substrate detection chip and its manufacture method, belong to micro-fluidic paper substrate chip field, including paper substrate chip, detection reagent scribbled on the paper substrate chip, the paper substrate chip is sprayed with test agent to be checked after scribbling detection reagent again.One kind provided by the invention can scale paper substrate detection chip and its manufacture method; the manufacturing cost of this method paper substrate chip is low, and reaction rate is fast, and precision is high; realization large-scale production can also be combined with mechanization simultaneously, disclosure satisfy that demand of the remote districts to disease examination.
Description
Technical field
The invention belongs to micro-fluidic paper substrate chip field, and relating generally to one kind can scale paper substrate detection chip and its manufacture
Method.
Background technology
Clinical examination, refer to (can be clinical laboratory in hospital, laboratory, can also be independent by clinical labororatory
Inspection institute) for a series of experiments room detection work of clinical medicine offer and the result of project, the diagnosis for disease.Clinic inspection
Test is observation, physics, chemistry, instrument by visual observation by samples such as the blood of patient, body fluid, secretion, excreta and casts
Or molecular biology method detection, and emphasize to examining overall process to take tight quality management (before analysis, in analysis, after analysis)
Measure is to ensure quality inspection；So as to provide valuable experimental data for clinic, for patient.Clinical examination is one multidisciplinary
Interpenetrate, the comprehensive application branch of learning of mixing together, be related to chemistry, physics, biology, biochemistry, immunology, micro- life
The subjects such as thing, physiology, pathology, science of heredity, molecular biology, statistics and multi-door clinical medicine.Present clinical inspection
The medical personnel for needing special instrument and equipment and specialty mostly are tested, in some remote districts due to the shortage of instrument and equipment,
Often cannot timely it detect.
At present, paper has obtained comprehensive popularization as a kind of cheap material in the world.With paper substrate micro-fluidic chip
The blood of patient, body fluid, secretion, excreta and cast etc., which are detected, can effectively solve the disease of remote districts
Monitoring problem.Paper substrate micro-fluidic chip quickly, is as a result easily observed, while easily destruction will not cause environment with efficiently simultaneously
The advantages of pollution.
Chinese patent CN103394384A discloses a kind of paper substrate micro-fluidic chip, including paper substrate chip and paper substrate egative film,
Described paper substrate chip has microfluidic channel, and described paper substrate egative film is correspondingly bonded in described in the bottom of the paper substrate chip
Paper substrate chip, paper substrate egative film make crosslinking curing processing through liquid glue respectively.The invention also discloses a kind of three-dimensional paper substrate micro-fluidic
Chip, formed by described paper substrate micro-fluidic chip drawn or folding.Paper substrate micro-fluidic chip described in the invention have into
The advantages that this is low, preparation is simple, liquid flowing is controllable, tests suitable for microfluid analysis.But the paper substrate micro-fluidic chip can not
Mass produced, so as to define its effect.
Chinese patent CN103792354A disclose it is a kind of detect antibody of HCV micro-fluidic paper substrate chip and
Its preparation method.Multiple miniature sense channels are included according to the micro-fluidic paper substrate chip of the present invention, each passage is coated with core respectively
Heart antigen, antigen, antigen, antigen and combinations thereof.The detectability of multichannel allows micro-fluidic paper substrate chip to carry out simultaneously
The detection of multiple immunization programs.By the examination of integrated micro on micro-fluidic paper substrate chip and test is made a definite diagnosis, is significantly simplified
Complicated diagnosis process, so as to greatly improve diagnosis efficiency.This invention is related to ingenious, and it is very accurate as a result to detect, greatly simple
The detection of hepatitis C patient is changed, multiple immunization programs can be detected simultaneously, improve detection efficiency.But the miniflow of the invention
It is more complicated to control paper substrate chip and preparation method thereof, it is difficult to popularize in an all-round way.
The invention discloses a kind of vertical paper substrate micro-fluidic detection chip and its preparation side by Chinese patent CN105879937A
Method, step is that paper is cut into paper slip, or paper slip is patterned into the paper slip with shape using the mode of shearing in advance, two-sided
Sticker is rolled into paper roll in paper slip both sides, by paper slip use paper pen of spreading out, and uses double faced adhesive tape to fix paper roll, paper passage is formed, by paper roll
It is fixed in substrate and makes two dimension or three-dimensional vertical paper substrate micro-fluidic detection chip, vertical chip has that cost is low, operation
Simply, without heating, without sealing modeling, the characteristics of flow velocity is controllable, high resolution, the biochemical Site Detection available for low cost.But
It is that the manufacture method of the invention is difficult to mechanized production, so being difficult to be promoted.
Traditional micro-fluidic chip is for base material with glass, silicon chip, high molecular polymer etc., is existed by technologies such as photoengravings
Microchannel, and integrated micro-valve door and Micropump are built on these base materials, forms micro-fluidic chip.However, this micro-fluidic chip
Prepare and complex operation be present, it is necessary to the defects of the process environments of expensive equipment and harshness, seriously limit micro-fluidic chip
Promotion and application.In face of the disease examination problem of patient from far-off regions, a kind of cost is low, it is simple to operate, without heating, need not
Envelope is moulded, flow velocity is controllable, high resolution paper substrate micro-fluidic chip active demand.
The content of the invention
The problem of current disease from far-off regions is difficult to detection is combined for the deficiencies in the prior art, the present invention provides
One kind can scale paper substrate detection chip and its manufacture method, the paper substrate micro-fluidic chip cost is low, it is simple to operate, need not plus
Heat, without sealing modeling, flow velocity is controllable, high resolution, while can realize that mechanization mass produces, can be widely applied it makes
With bringing Gospel for the disease surveillance of Disease from far-off regions.
Design of the present invention is：One kind can scale paper substrate detection chip and its manufacture method, including paper
Base chip 1, scribbles detection reagent 2 on the paper substrate chip 1, the paper substrate chip 1 scribble again be sprayed with after detection reagent 2 it is to be detected
Reagent 3.
In design provided by the invention, the paper substrate chip can be filter paper, printing paper, paperboard, NC Nitroncellulose
Any one in paper made of film or glucan.
In design provided by the invention, color reaction be able to must occur for detection reagent 2 and test agent to be checked 3
Material.
In design provided by the invention, the mode that detection reagent 2 is scribbled on the paper substrate chip 1 is to utilize plotter
Or writing machine is carried out.
In design provided by the invention, step is；
Step 1, a water core pen 4 is taken, cleaned, drying；
Step 2, detection liquid 3 is fitted into the water core pen 4 made by step 1；
Step 3, the water core pen 4 made by step 2 is painted using plotter or writing machine on paper substrate chip 1
Pattern or writing words；
Step 4, prepare liquid 3 is sprayed on to the paper substrate chip 1 made by step 3 and is painted with pattern or the area with word
Domain, drying, its color change is observed after 1~10 minute.
In design provided by the invention, the pen core of the water core pen 4 is 0.5mm~0.75mm.
The present invention has advantages below：
One kind provided by the invention can scale paper substrate detection chip and its manufacture method, the manufacture of this method paper substrate chip
Cost is low, and reaction rate is fast, and precision is high, while can also be combined realization large-scale production with mechanization, disclosure satisfy that remote
Demand of the area to disease examination.
The invention will be further described below in conjunction with the accompanying drawings.
Brief description of the drawings
Fig. 1 filter paper
Fig. 2 writing machines are glycoxidative with the grape for filling 2,2- connection nitrogen-two (3- ethyls-benzothiazole -6- sulfonic acid) di-ammonium salts
Enzyme detection liquid writes the filter paper of word；
Fig. 3 uses writing machine the grape glycosyloxy for filling 2,2- connection nitrogen-two (3- ethyls-benzothiazole -6- sulfonic acid) di-ammonium salts
Change enzyme detection liquid and write word, then spray the filter paper of glucose solution；
Embodiment
Below in conjunction with accompanying drawing of the present invention and specific design scheme, the design in the embodiment of the present invention is carried out clear
Chu, it is fully described by, it is clear that described embodiment is only the part of the embodiment of the present invention, rather than whole implementation
Example.Based on the embodiment in patent of the present invention, those of ordinary skill in the art are obtained under the premise of creative work is not paid
The every other embodiment obtained, belong to the scope of this patent protection.
Embodiment 1
537.7 milligrams of 2,2- connection nitrogen-two (3- ethyls-benzothiazole -6- sulfonic acid) is first weighed, is dissolved in 39.2 milliliters of grapes
In oxidase solution, glucose detection liquid is made into.Take 0.70mm water core pen to clean, dry, the grape that will be prepared
Sugar detection liquid is fitted into treated water core pen.By manufactured water core pen on writing machine, then take a clean filter paper such as
Fig. 1, according to the word of setting, word is finished writing on filter paper with writing machine, as shown in Figure 2.Glucose solution is sprayed, the color after 3min
Change such as Fig. 3.
Every technical staff's notice：Although the present invention describes according to above-mentioned embodiment, of the invention
Invention thought be not limited to that invention, any repacking with inventive concept, will all include this patent protection of the patent right
In the range of.
Claims (6)
1. one kind can scale paper substrate detection chip and its manufacture method, it is characterised in that including paper substrate chip (1), the paper
Detection reagent (2) is scribbled on base chip (1), the paper substrate chip (1) is sprayed with test agent to be checked after scribbling detection reagent (2) again
(3)。
2. one kind according to claim 1 can scale paper substrate detection chip and its manufacture method, it is characterised in that described
Paper substrate chip can be filter paper, printing paper, paperboard, any one in paper made of nitrocellulose membrane or glucan.
3. one kind according to claim 1 can scale paper substrate detection chip and its manufacture method, it is characterised in that detection
Reagent (2) and test agent to be checked (3) must be the materials that color reaction can occur.
4. one kind according to claim 1 can scale paper substrate detection chip and its manufacture method, it is characterised in that described
The mode that detection reagent (2) is scribbled on paper substrate chip (1) is to utilize plotter or writing machine progress.
5. one kind according to claim 1 can scale paper substrate detection chip and its manufacture method, it is characterised in that step
For；
Step 1, a water core pen (4) is taken, cleaned, drying；
Step 2, detection liquid (3) is fitted into the water core pen made by step 1 (4)；
Step 3, the water core pen (4) made by step 2 is painted using plotter or writing machine on paper substrate chip (1)
Pattern or writing words；
Step 4, prepare liquid (3) is sprayed on to the paper substrate chip (1) made by step 3 and is painted with pattern or the area with word
Domain, drying, its color change is observed after 1~10 minute.
6. manufacture method according to claim 5, it is characterised in that the pen core of the water core pen (4) be 0.5mm~
0.75mm。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710760443.4A CN107384772A (en) | 2017-08-30 | 2017-08-30 | One kind can scale paper substrate detection chip and its manufacture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710760443.4A CN107384772A (en) | 2017-08-30 | 2017-08-30 | One kind can scale paper substrate detection chip and its manufacture method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107384772A true CN107384772A (en) | 2017-11-24 |
Family
ID=60347183
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710760443.4A Pending CN107384772A (en) | 2017-08-30 | 2017-08-30 | One kind can scale paper substrate detection chip and its manufacture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107384772A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975471A (en) * | 2019-02-27 | 2019-07-05 | 海南医学院 | A kind of preparation method of the information pattern paper base sensor of detection of heavy metal ion |
-
2017
- 2017-08-30 CN CN201710760443.4A patent/CN107384772A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975471A (en) * | 2019-02-27 | 2019-07-05 | 海南医学院 | A kind of preparation method of the information pattern paper base sensor of detection of heavy metal ion |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171124 |
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| WD01 | Invention patent application deemed withdrawn after publication |