CN107384756A - A kind of collection device and method of essence spice for cigarette contaminating microorganisms - Google Patents
A kind of collection device and method of essence spice for cigarette contaminating microorganisms Download PDFInfo
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- CN107384756A CN107384756A CN201710648339.6A CN201710648339A CN107384756A CN 107384756 A CN107384756 A CN 107384756A CN 201710648339 A CN201710648339 A CN 201710648339A CN 107384756 A CN107384756 A CN 107384756A
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- bottom shelf
- cigarette
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention is a kind of collection device of essence spice for cigarette contaminating microorganisms.It includes casing-upper half and the bottom shelf half that can be combined together；Wherein described casing-upper half has sample inlet and closure；The bottom shelf half has the filter membrane and support that can be combined.The collection device of the essence spice for cigarette contaminating microorganisms of the present invention is simple in construction, it is easy to operate, cost is cheap, influence of all kinds of essence and flavoring agent components to follow-up microbiological analysis can be effectively removed, solves the problems, such as during microorganism-collecting that sample shifts repeatedly and is caused damage with the multipair cell of operating procedure；Meanwhile the device can high pressure sterilizing, and small volume is convenient is operated in superclean bench, so as to greatly reduce the risk of inoculating microbe pollution.
Description
Technical field
The present invention relates to the collection technique field of microbial cell, specifically a kind of essence spice for cigarette contaminating microorganisms
Collection device and method.
Background technology
Microorganism pollution is prevalent in each ring such as the production and processing, transport and preservation of food, medicine and cosmetics etc.
Section, it is one of important hidden danger for influenceing Product quality and safety.High throughput sequencing technologies overcome the shortcomings that tradition culture, greatly
Deep understanding of the people to microorganism species structure and diversity in these samples, promote the validity of prevention and control measure formulation.
Wherein, the collection of microorganism is first and unusual the key link in high throughput sequencing technologies in sample, directly affects bacterium
The reliability of group structure and diversity analysis result.At present, mainly use centrifuges, is thin for the collection of contaminating microorganisms in these samples
The mode that the technical combinations such as membrane filtration, vibrating dispersion are selected, but operating procedure is more, it is necessary to use the instrument such as filtering, flushing, vibration
Device, and the transfer that pipette progress microorganism is used for multiple times can cause damage again.
Essence spice for cigarette is the indispensable important source material of production of cigarettes, has and assigns cigarette product individual style, changes
Kind flue gas mass, promote the effect such as product acceptability, mixed by what the allotment of a variety of spices, appropriate solvent and other compositions formed
Compound.In links such as the productions and accumulating of essence spice for cigarette, quality decline caused by microorganism pollution be present so as to cause to produce
Product are scrapped.High throughput sequencing technologies are advantageous to more comprehensively grasp microorganism that may be present in essence and flavoring agent, are that essence is fragrant
The rotten prevention and control of material provide Risk-warning in advance and formulate effective prevention and control measure, ensure Product quality and safety.With other classes
The sample of type is compared, and the component of essence spice for cigarette is increasingly complex various, the various operating procedure of existing microorganism-collecting method
Loss is inevitably resulted in multiple transfer, the understanding to contaminating microorganisms is influenceed, in addition, some groups during microorganism-collecting
Residual is divided to be had an impact to follow-up pcr amplification reaction etc..
The content of the invention
In order to solve the above technical problems, the purpose of the present invention aims to provide a kind of receipts of essence spice for cigarette contaminating microorganisms
Acquisition means and method, during removing influence and microorganism-collecting of all kinds of essence and flavoring agent components to follow-up microbiological analysis
Sample shifts the problem of being caused damage with the multipair cell of operating procedure repeatedly；Meanwhile the device can high pressure sterilizing, and
Small volume is convenient to be operated in superclean bench, so as to greatly reduce the risk of inoculating microbe pollution.
Technical scheme is as follows：
A kind of collection device of essence spice for cigarette contaminating microorganisms is configured to：The casing-upper half and lower half that can be combined together
Portion's housing；Wherein described casing-upper half has sample inlet and closure；The bottom shelf half has the filter membrane that can be combined
And support.
Described casing-upper half and the bottom shelf half are semiellipse cavity.
Pass through the threaded connection of inner walls between described casing-upper half and the bottom shelf half.
Filter paper and the filter membrane exterior bottom is connected together with support, and support is bilayer.
The support is no less than 3 per layer number.
For the filter paper close to the oral area of bottom shelf half, aperture is 35-45 μm.
For the filter membrane close to the bottom of bottom shelf half, aperture is 0.22-0.45 μm.
30-50mg beades can be placed as needed in the bottom shelf half.
Collection device is used for the method that essence spice for cigarette contaminating microorganisms are collected：
A. casing-upper half is connected with without filter membrane with the bottom shelf half of support, opens the closure of casing-upper half, from
Sample inlet is sequentially placed into bead and testing sample, covers closure, and fully vibration makes sample dispersion, hunting speed 140-
180rpm, duration of oscillation 10-15min；
B. casing-upper half is placed below, makes sample in casing-upper half, bottom shelf half is separated, by the upper half containing sample
Portion's housing is connected with the bottom shelf half containing filter paper, filter membrane and support, wherein being close to the filter paper aperture of bottom shelf half oral area
35-45 μm, the filter sizes close to bottom are 0.22-0.45 μm, and casing-upper half is placed in top, sampfe order is passed through filter paper
And filter membrane；
C. casing-upper half and bottom shelf half are separated, takes out the filter paper close to bottom shelf half oral area successively, then by the first half
Housing connects with bottom shelf half, adds aseptic deionized water 10-20mL from sample inlet, collected microorganism is i.e. in filter membrane
On, for follow-up microbiological analysis.
The present invention is simple in construction, and easy to operate, cost is cheap, can effectively remove all kinds of essence and flavoring agent components to follow-up micro-
The influence of bioanalysis, sample shifts what is caused damage with the multipair cell of operating procedure repeatedly during solving microorganism-collecting
Problem；Meanwhile the device can high pressure sterilizing, and small volume is convenient is operated in superclean bench, so as to drop significantly
The risk of low inoculating microbe pollution.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention.
Fig. 2 is the bottom shelf half structural representation of the present invention.
Wherein reference implication is as follows：
1st, casing-upper half；2nd, bottom shelf half；3rd, screw thread；4th, sample inlet；5th, closure；6th, bead；7th, filter membrane；8th, prop up
Frame；9th, coupling part.
Embodiment
As shown in Figure 1, 2, device of the present invention is configured to：The casing-upper half 1 and lower half shell that can be combined together
Body 2, casing-upper half 1 have sample inlet 4 and closure 5, and bottom shelf half 2 has two kinds, and a kind is with filter paper 7, filter membrane 8
And the support 9 that energy is in combination, casing-upper half 1 and the bottom shelf half 2 are semiellipse cavity, pass through inner walls
Screw thread 3 connects；Support in bottom shelf half 2 is bilayer, and every layer of number of holders is no less than 3；Filter paper 7 and the outside bottom of filter membrane 8
Portion is connected together with support 9；Filter paper 7 is close to the oral area of bottom shelf half 2, and aperture is 35-45 μm, and filter membrane 8 is close to lower half shell
The bottom of body 2, aperture are 0.22-0.45 μm；30-50mg beades 6 can be placed in bottom shelf half 2 as needed.
Casing-upper half 1 and bottom shelf half 2 without filter membrane and support are connected first during use, open first half shell
The closure 5 of body 1, bead 6 and testing sample are sequentially placed into from sample inlet 4, covers closure 5, fully vibration makes sample
It is scattered.
Casing-upper half 1 makes sample in lower section in casing-upper half 1, bottom shelf half 2 is separated, by the upper half containing sample
Portion's housing 1 and bottom shelf half 2 containing filter membrane and support connect, and casing-upper half 1 up makes sampfe order pass through 30-50 μm
With 0.22-0.45 μm of double-layered filtration film.
Casing-upper half 1 and bottom shelf half 2 are separated, takes out the filter membrane close to the oral area of bottom shelf half 2 and bottom successively,
Collected microorganism is the filter membrane close to bottom, for follow-up microbiological analysis.
Application Example 1:
Casing-upper half 1 and bottom shelf half 2 without filter paper, filter membrane and support are connected first during use, open the first half
The closure 5 of housing 1, bead 6 and testing sample are sequentially placed into from sample inlet 4, covers closure 5, fully vibration makes sample
Product are dispersed, hunting speed 140rpm, duration of oscillation 15min；
Then it is casing-upper half 1 is placed below, make sample in casing-upper half 1, separate bottom shelf half 2, sample will be contained
Casing-upper half 1 be connected with the bottom shelf half 2 containing filter paper 7, filter membrane 8 and support 9, casing-upper half 1 be placed in top, make sample
Product sequentially pass through filter paper and filter membrane；
Casing-upper half 1 and bottom shelf half 2 are separated again, take out the filter paper 7 close to bottom shelf half 2 oral area, then by the first half
Housing 1 and bottom shelf half 2 connect, and add aseptic deionized water 10-20mL from sample inlet 4, collected microorganism exists
On filter membrane 8, for follow-up microbiological analysis.
Application Example 2:
Sample dispersion hunting speed 160rpm, duration of oscillation 12min in the present embodiment.Other processes are identical with application examples 1.
Application Example 3:
Sample dispersion hunting speed 1180rpm, duration of oscillation 10min in the present embodiment.Other processes are identical with application examples 1.
Claims (9)
1. a kind of collection device of essence spice for cigarette contaminating microorganisms, it is characterised in that including the upper half that can be combined together
Portion's housing（1）And bottom shelf half（2）；Wherein described casing-upper half（1）With sample inlet（4）And closure（5）；It is described
Bottom shelf half（2）Inside there is filter paper（7）And filter membrane（8）, filter paper（7）And filter membrane（8）By support（9）Supporting.
2. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that the upper half
Portion's housing（1）With the bottom shelf half（2）It is semiellipse cavity.
3. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that the upper half
Portion's housing（1）With the bottom shelf half（2）Between pass through the screw threads of inner walls（3）Connection.
4. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that the filter paper
（7）And filter membrane（8）Exterior bottom and support（9）It is connected together, support（9）For bilayer.
5. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that the support
（8）3 are no less than per layer number.
6. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that the filter paper
（7）Close to bottom shelf half（2）Oral area, aperture be 35-45 μm.
7. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that the filter membrane
（8）Close to bottom shelf half（2）Bottom, aperture be 0.22-0.45 μm.
8. the collection device of essence spice for cigarette contaminating microorganisms according to claim 1, it is characterised in that described
Bottom shelf half（2）30-50mg beades can be inside placed as needed（6）.
9. the collection device described in claim 1 is used for the method that essence spice for cigarette contaminating microorganisms are collected, it is characterised in that
It comprises the following steps：
A. by casing-upper half（1）With the bottom shelf half without filter membrane and support（2）Connection, open casing-upper half（1）'s
Closure（5）, from sample inlet（4）It is sequentially placed into bead（6）And testing sample, cover closure（5）, fully vibrating makes sample
Product disperse, hunting speed 140-180rpm, duration of oscillation 10-15min；
B. casing-upper half（1）It is placed below, make sample in casing-upper half（1）It is interior, separate bottom shelf half（2）, sample will be contained
The casing-upper half of product（1）With the bottom shelf half containing filter paper, filter membrane and support（2）Connection, wherein close to bottom shelf half（2）
The filter paper of oral area（7）Aperture is 35-45 μm, the filter membrane close to bottom（8）Aperture is 0.22-0.45 μm, casing-upper half（1）Put
In top, sampfe order is set to pass through filter paper and filter membrane；
C. casing-upper half is separated（1）And bottom shelf half（2）, take out successively close to bottom shelf half（2）The filter paper of oral area
（7）, then by casing-upper half（1）And bottom shelf half（2）Connection, from sample inlet（4）Add aseptic deionized water 10-
20mL, collected microorganism is i.e. in filter membrane（8）On, for follow-up microbiological analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710648339.6A CN107384756B (en) | 2017-08-01 | 2017-08-01 | Device and method for collecting tobacco essence and spice polluted microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710648339.6A CN107384756B (en) | 2017-08-01 | 2017-08-01 | Device and method for collecting tobacco essence and spice polluted microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107384756A true CN107384756A (en) | 2017-11-24 |
| CN107384756B CN107384756B (en) | 2023-09-15 |
Family
ID=60344581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710648339.6A Active CN107384756B (en) | 2017-08-01 | 2017-08-01 | Device and method for collecting tobacco essence and spice polluted microorganisms |
Country Status (1)
| Country | Link |
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| CN (1) | CN107384756B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536376A (en) * | 2018-12-14 | 2019-03-29 | 江苏环保产业技术研究院股份公司 | A kind of microorganisms in water original-position collecting device |
| CN113817608A (en) * | 2021-10-18 | 2021-12-21 | 福建中烟工业有限责任公司 | Separation and enrichment method of microorganisms on surface of alcoholized tobacco leaf |
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| CA2337987A1 (en) * | 2000-02-25 | 2001-08-25 | Elmex Limited | Membrane filtration system |
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| CN113817608A (en) * | 2021-10-18 | 2021-12-21 | 福建中烟工业有限责任公司 | Separation and enrichment method of microorganisms on surface of alcoholized tobacco leaf |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107384756B (en) | 2023-09-15 |
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