CN107377024A - Micro-fluidic syringe filter and its application method - Google Patents
Micro-fluidic syringe filter and its application method Download PDFInfo
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- CN107377024A CN107377024A CN201710816147.1A CN201710816147A CN107377024A CN 107377024 A CN107377024 A CN 107377024A CN 201710816147 A CN201710816147 A CN 201710816147A CN 107377024 A CN107377024 A CN 107377024A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
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Abstract
The invention discloses a kind of micro-fluidic syringe filter and its application method, the filter carries out enrichment method using microflow control technique to micron particles or cell, its Y type bifurcated flow-channel for being internally provided with the fluid channel of spiral extension and being connected with fluid channel, the particulate of sample liquid is in fluid channel in addition to the drag force by flow direction, also suffer from the inertia lift and Dean drag perpendicular to main flow direction, under the coupling of the two horizontal forces, particulate will gradually focus to the wall close to spiral center side, formation rule arranges beam, and flowed out along the inner branch of Y type bifurcated flow-channels, so as to be collected into the sample liquid of concentration；And fluid channel and Y type bifurcated flow-channels are respectively provided with the volume at grade, effectively reducing runner occupancy in the present invention, are advantageous to the miniaturization of filter；The application method of the filter is simple, is used suitable for the laboratory environment of field and low hardware configuration.
Description
Technical field
The present invention relates to a kind of syringe filter, is used for micron particles and biological cell rapid concentration more particularly to one kind
Micro-fluidic syringe filter and its application method.
Background technology
In fluid sample the shaped solid composition such as micron particles concentration and enrichment be modern biochemical and medical laboratory in
The sample pretreatment step of most frequent progress.For example, the mark of cell needs to realize unnecessary labelled reagent by concentration operation
With the separation for completing mark cell sample；The cracking needs of overall background red blood cell are realized uncracked white thin by concentrating in blood
Born of the same parents etc. and the separation of bib；The quick of great infectiousness epidemic situation, effective treatment are needed from large volume ambient source sample liquid
Or rapid concentration is enriched with target pathogen in physiological fluid, to make a definite diagnosis the state of an illness and develop vaccine.For the micro-nano of great challenge
Material fast enriching and concentration, the main method of report include high speed centrifugation and fracture filtration etc..High speed centrifugation method pass through by
Centrifugal acceleration is raised above hundreds and thousands of times of acceleration of gravity, is settled down to it so as to increase the sinking speed of solid material
Bottom of the tube is centrifuged, and the concentration of pending sample is lifted by sopping up supernatant.However, high speed centrifugation is needed by costliness
Centrifugation apparatus, operator need to be according to the density contrasts between solids to be concentrated and suspension liquid come reasonable selection centrifugal speed, and mistake
High centrifugal speed can cause irreversible damage to flexible biological sample.Drawbacks described above causes high speed centrifugation method and not applied to
In field, amateur Biochemical Lab etc., low hardware configuration environment uses, and can not the extremely dilute flexible biological sample of concentration for the treatment of.
Compared to high speed centrifugation method, fracture filtration is a kind of more direct micro Nano material enrichment method for concentration, this method by containing
The film of specific dimensions microcellular structure obstructs solid constituent to be concentrated, and filter walks blank solution to lift the concentration of sample.But specific chi
Very little microporous barrier can only obstruct size and be more than the material in aperture, therefore need to frequently change filter membrane to adapt to the mistake of different sized objects
Filter concentration.In addition, the recovery of solid constituent is always that the arduousness that fracture filtration method for concentration faces is chosen on the blocking of fenestra and film
War.Syringe filter product on the market is the principle using above-mentioned fracture filtration at present, but is mainly used in the mistake of fluid sample
Filter off it is miscellaneous, do not consider obstruct solid constituent recovery.
Microflow control technique since the last century 90's is suggested, because its cost is low, manipulation precision is high and needed for sample
Numerous advantages such as few are measured, have been widely used in transporting, capture, sort, be enriched with and detecting for micro Nano material.But have not yet to see
Occur with micron particles/cell concentration enriched products of microflow control technique.
The content of the invention
Goal of the invention：The defects of in order to overcome prior art, the invention provides a kind of micro-fluidic syringe filter, the filter
Head realizes that the micron particles of certain size scope and the rapidly, continuously stream of biological cell concentrate by the microfluid inertia effect.
Technical scheme：A kind of micro-fluidic syringe filter of the present invention, including body, described body one end are provided with sample
Product entrance, the other end is provided with sample export and blank solution exports；Body interior is provided with feed pathway, liquid storage pool, fluid channel, Y types
Bifurcated flow-channel, concentrate passage and blank solution passage；The feed pathway connects the sample export and liquid storage pool；It is described micro-
Runner connects the liquid storage pool, and is extended spirally clockwise or counter-clockwise using liquid storage pool as starting point；The Y types bifurcated flow-channel
For one-in-and-two-out formula, including straight channel, the first turnout and the second turnout, the port of export phase of the straight channel and fluid channel
Even；The concentrate passage connects first turnout and sample export, blank solution passage connect second turnout and
Blank solution exports；In order to reduce filter size, the liquid storage pool, fluid channel and Y type bifurcated flow-channels are disposed on the same plane.
Operation principle of the present invention：Pushing syringe, sample liquid is injected from sample inlet with certain flow, in sample liquid
Micron particles or biological cell, in addition to by the drag force along sample liquid flow direction, due to the master of the microfluid inertia effect
Lead, also by by the inertia lift and Dean drag perpendicular to main flow direction.In the coupling of the two horizontal forces
Under, the micron particles or biological cell of fluid channel arrival end random dispersion will be focused to close internal face in the port of export, also
I.e. close to the wall of spiral center side, so as to the arrangement beam of formation rule, and first point of Y types bifurcated flow-channel in the inner part is flowed through
Turnout, concentrate passage, and finally export and collect from sample export, and blank solution then by Y types bifurcated flow-channel in the outer part the
Two turnouts, blank solution passage is flowed through, and export and export eventually through blank solution.So the removal of blank solution will cause sample
Solids concentration is obviously improved in liquid.For etc. parting Y runner exits, once-through operation can lift concentration in principle to 2 times, also
The width that branch in Y types bifurcated flow-channel can be reduced lifts effect to lift the concentration of single concentration.
To ensure, micron particles or biological cell can smoothly be arranged in rule in fluid channel exit region under specific flow velocity
The relation of pencil, the cross sectional dimensions of fluid channel and particle size to be concentrated needs to meet：
0.07≤ap/Lc≤0.6
Wherein, apFor the maximum gauge of concentrating particulate；Lc is the characteristic size of fluid channel.
Corresponding to above-mentioned micro-fluidic syringe filter, present invention likewise provides a kind of technical scheme of application method, make
It is as follows with flow：
(1) according to the micro-fluidic syringe filter of the size of object to be concentrated and scattered Sexual behavior mode appropriate size；
(2) syringe of appropriate volume is chosen, draws sample liquid to be concentrated, and injector head is inserted into sample inlet 1；
(3) sample export 2 and blank solution outlet 3 are connected into conventional syringe syringe needle or the pipeline of other female Luers respectively
Connector, different liquids storage area is drained to respectively；
(4) medical injection pump or hand push mode at the uniform velocity pushing syringe are used, until liquid has all pushed away in syringe；
(5) liquid that sample export 2 is collected is loaded into syringe again, and repeats the above steps (1) to step (4),
Further to lift the concentration of sample liquid.
Beneficial effect：Micro-fluidic syringe filter of the present invention, sets spiral fluid channel in sample flow path,
The regularly arranged focusing of particle is realized by the inertia effect of microfluid under specific flow velocity, and will be dense by Y types bifurcated flow-channel
Sample liquid and blank solution the shunting export of contracting, it is achieved thereby that the micron particles of certain size scope and biological cell it is quick,
Continuous stream concentrates.Liquid storage pool, fluid channel and Y type bifurcated flow-channels are arranged on same plane, effectively reduce the volume of filter, have
Beneficial to the miniaturization of filter.The filter using when be not susceptible to block, low manufacture cost simple in construction, can disposably abandon makes
With, and operating method is simple, suitable for the operating environment such as field and low hardware configuration laboratory.
Brief description of the drawings
Fig. 1 is the explosive mechanisms schematic diagram of micro-fluidic syringe filter；
Fig. 2 is the mounted inside schematic diagram of micro-fluidic syringe filter；
Fig. 3 is the multiple concentration microphoto to 10 micron polystyrene microspheres；
Fig. 4 is the multiple concentrated effect block diagram to 10 micron polystyrene microspheres；
Fig. 5 is the cell concentration change curve under different operating flow to human breast cancer cell；
Fig. 6 is the cell recoveries block diagram under different operating flow to human breast cancer cell；
Fig. 7 is the active testing microphoto of the cell after the processing of micro-fluidic syringe filter.
Embodiment
The achievable mode of the present invention is described in further details below in conjunction with the accompanying drawings.
Micro-fluidic syringe filter of the present invention, is a kind of miniaturization filter for being directly connected to syringe, the filter
Head uses microflow control technique, realizes micron particles and biological cell rapid concentration.
As shown in figure 1, a kind of micro-fluidic syringe filter, its body construction includes upper lid 10, thickening apparatus 11 and close
Packing 12, upper lid 10 are closely connected with thickening apparatus 11, and sealing gasket 12 is arranged between lid 10 and thickening apparatus 11.Wherein,
Upper lid 10 and thickening apparatus 11 can choose the materials such as medical plastic, stainless steel and be added by injection molding, machining, the three-dimensional material etc. that increases
Work mode manufactures；Sealing gasket 12 can choose the making of the flexible materials such as silica gel, rubber or dimethyl silicone polymer.Sealing gasket 12 is logical
The form and the bonded seal of thickening apparatus 11, upper lid 10 for crossing glue bonding or pressure compaction can pass through the shapes such as screw thread, buckle
Formula is fastenedly connected with thickening apparatus 11, and provides pressure to sealing gasket 12.The filter is illustrated in Fig. 2 by taking threaded form as an example
The mounted inside structure of head, equally can also be made the product connected in a manner of buckle, welding etc., it is, of course, also possible to be beaten by 3D
Print is integrally formed.
Each design of part for further refining the filter is formed, and its body also includes setting sample inlet 1 at one end, set
The sample export 2 and blank solution put in the other end export 3.That is, sample inlet 1 is arranged in the upper end of lid 10, sample
Product outlet 2 and blank solution outlet 3 are arranged in the lower end surface of thickening apparatus 11.Sample inlet 1, sample export 2 and blank
Liquid outlet 3 is standard luer fittings design, and conveniently with syringe or on the market, medical tubing joint is connected；Pay particular attention to
The minimum spacing of the central axis of central axis and the blank solution outlet 3 of sample export 2, in order to both while loading injector
Syringe needle either other standards female Luer pipeline or can also be set according to the sample cavity spacing of 96 orifice plates etc..Body interior
It is additionally provided with feed pathway 4, liquid storage pool 5, fluid channel 6, Y types bifurcated flow-channel 7, concentrate passage 8 and blank solution passage 9.Feed liquor leads to
Road 4 connects sample inlet 1 and liquid storage pool 5, and feed pathway 4 is connected by the through hole 42 on the duct 41 and sealing gasket 12 of upper lid
Connect and form.So that main body in Fig. 2 is in the filter of cylinder as an example, sample inlet 1, duct 41 are consistent with the central axis of through hole 42,
Central axis all with cylinder is consistent.
Liquid storage pool 5, fluid channel 6, Y types turnout 7, concentrate passage 8 and blank solution passage 9 are all disposed within thickening apparatus
On, in order to reduce the volume of filter, meet the requirement of miniaturization, liquid storage pool 5, fluid channel 6 and Y type bifurcated flow-channels are arranged at
The upper surface of thickening apparatus 11, that is, be arranged on the end face of the top side of lid 10 of thickening apparatus 11；And concentrate passage 8 and sky
White liquor passage 9 is the circular hole for directly running through thickening apparatus 11.The overall covering fluid channel of sealing gasket 12, Y types bifurcated flow-channel and
The entrance circular hole of concentrate passage 8 and blank solution passage 9, bonded seal form the runner of closing.Liquid storage pool 5 is round groove,
It is arranged on cylindrical center's axis of thickening apparatus, the cross-sectional diameter of liquid storage pool 5 is more than or equal to the cross-sectional diameter of through hole 42,
The connected pore channel 41 of through hole 42 and liquid storage pool 5, liquid storage pool 5 connects fluid channel 6, so that the sample liquid energy injected by sample inlet 1
Accumulated in liquid storage pool 5, flow into fluid channel 6 under pressure.
Fluid channel 6 is starting point with liquid storage pool 5, clockwise or counter-clockwise spiral extension.According to micro-fluidic fluid force
Principle is learned, can also set and be arcuately curved or the runner of other forms.For the ease of processing and manufacturing, by the horizontal stroke of fluid channel 6
Cross sectional shape is arranged to low depth-to-width ratio rectangle, is, and the cross section of fluid channel 6 is width than highly much larger rectangle.Spiral
The design of the internal diameter, adjacent channels spacing of the fluid channel 6 of shape is considered as sealing intensity, and too small spacing can cause micro-fluidic injection
Leakage damage occurs under high pressure for device filter, and flow channel length also should be ensured that particulate can be with when migrating to 6 port of export of fluid channel
Transverse focusing bunchy.
To ensure, micron particles or biological cell can be smoothly arranged in the exit region of fluid channel 6 under specific flow velocity
Regular pencil, the ratio between particle diameter and flow path features size need to meet to be more than or equal to 0.07, and should be less than or equal to 0.6, because
When more than 0.6, runner easily blocks.Therefore, stream can be used with reference to above-mentioned empirical equation, the characteristic size of fluid channel 6
The height in road is estimated.For the micro-fluidic syringe filter of stationary flowpath size, the particle size range that it can be concentrated is
0.07 times of runner is highly to 0.6 times of runner height.
Y types bifurcated flow-channel 7, set using one-in-and-two-out formula, including straight channel 71, the first turnout 72 and the second turnout
73；Straight channel 71 is the inlet channel of Y type bifurcated flow-channels, is connected to the end of fluid channel 6, and the first turnout 72 is arranged on by spiral shell
The inner branch of the side, as Y types bifurcated flow-channel 7 at shape runner center is revolved, the second turnout 73 is the outer of Y types bifurcated flow-channel 7
Side branch.Bundles of micron particles or biological cell are laterally converged still to collect in close to spiral center area into straight channel 71
The runner wall of domain side, when flowing to the fork node of Y types bifurcated flow-channel 7, separately flowed into from blank solution in different branches,
The micron particles or biological cell of concentration flow into the first bifurcated flow-channel 72 of inner side, and blank solution flows into the second bifurcated flow-channel 73.The
One bifurcated flow-channel 72 connects sample export 2 by concentrate passage 8, and the second bifurcated flow-channel 73 connects sky by blank solution passage 9
White liquor outlet 3, so that can each export collection after concentrate and blank solution shunting.
The process for using of micro-fluidic syringe filter of the present invention is as follows：
(1) according to the micro-fluidic syringe filter of the size of object to be concentrated and scattered Sexual behavior mode appropriate size；
(2) syringe of appropriate volume is chosen, draws sample liquid to be concentrated, and injector head is inserted into sample inlet 1；
(3) sample export 2 and blank solution outlet 3 are connected into conventional syringe syringe needle or the pipeline of other female Luers respectively
Connector, different liquids storage area is drained to respectively；
(4) medical injection pump or hand push mode at the uniform velocity pushing syringe are used, until liquid has all pushed away in syringe；
(5) liquid that sample export 2 is collected is loaded into syringe again, and repeats the above steps (1) to step (4),
Further to lift the concentration of sample liquid.
In order to examine the filter effect of micro-fluidic syringe filter of the present invention, it is highly 100 microns to have made runner,
Width of flow path is 500 microns of filter finished product.It is 10 micron polystyrene microspheres to treat concentrated granular, and size, which is floated, to be less than
15%, initial concentration 1.83*105Individual/milliliter, the sample liquid that about 8 milliliters of volume are concentrated.Sample liquid is loaded onto into volume is
10 milliliters of Dispoable medical syringe, driving flow are 2.5 ml/mins, i.e., per minute at the uniform velocity to inject 2.5 milliliters of samples
Liquid.Such as Fig. 3, sample liquid is concentrated three times using the filter, the concentrating sample liquid collected every time photo under the microscope, such as
Fig. 4, the sample export 2 obtained and the microballoon quantity block diagram of blank solution outlet 3 are counted using cell counter.By for the first time
N1 is concentrated, and the concentrate microballoon concentration that sample export 2 is collected is 4.37*105Individual/milliliter, the blank solution that blank solution outlet 3 is collected
Middle no microballoon, microballoon concentration lifts 2.38 times after once concentrating；The concentrate that sample export 2 is collected after second of N2 is concentrated
In microballoon concentration be 4.6 times of initial sample liquid；Grain in the concentrate that sample export 2 is collected after third time N3 concentrations
Sub- concentration is promoted to 7.59 times of initial sample liquid.Concentrated effect is notable.
Equally, the micro-fluidic syringe filter of above-mentioned size is used for the concentration of flexible biological cell, is breast in this experiment
Gland cell system MCF-7.Such as Fig. 5, in the case where operating the ml/min of flow 1.5 to 4.0 ml/mins, sample inlet 1 it is initial
The change curve of cell concentration in the concentrate that sample, sample export 2 are collected.As a result show, be 3 millis in sample driving flow
Liter/min when, the filter has a best concentrated effect, and cell concentration is very low in the concentrates that blank solution outlet 3 is collected, and
Cell concentration highest in the concentrate that sample inlet 2 is collected.Such as Fig. 6, original sample is removed using the concentrate cell number of sample export 2
Product liquid total cell number calculates cell recoveries under different flow, test result indicates that the filter is respectively provided with wider flow rates
Good cell concentration effect.In addition, the cell of recovery is further cultured for verify the micro-fluidic syringe filter of the present invention
Under the operator scheme for meeting its process for using, cytoactive will not be had an impact, such as Fig. 7, it continues for 36~96 hours
Photo is cultivated, recovery cell still has good activity in the case where highest tests flow velocity.
Claims (8)
- A kind of 1. micro-fluidic syringe filter, it is characterised in that：Including body, described body one end is provided with sample inlet (1), separately One end is provided with sample export (2) and blank solution outlet (3)；Body interior is provided with feed pathway (4), liquid storage pool (5), fluid channel (6), Y types bifurcated flow-channel (7), concentrate passage (8) and blank solution passage (9)；The feed pathway (4) connects the sample Entrance (1) and liquid storage pool (5)；The fluid channel (6) connects the liquid storage pool (5), and is that starting point is clockwise with liquid storage pool (5) Or counter-clockwise helical shape extension；The Y types bifurcated flow-channel (7) is one-in-and-two-out formula, including straight channel (71), the first turnout (72) it is connected with the second turnout (73), straight channel (71) with the port of export of the fluid channel (6)；The concentrate passage (8) First turnout (72) and sample export (2) are connected, blank solution passage (9) connects second turnout (73) and blank Liquid exports (3)；The liquid storage pool (5), fluid channel (6) and Y types bifurcated flow-channel (7) are disposed on the same plane.
- 2. micro-fluidic syringe filter according to claim 1, it is characterised in that：It is (10) and dense that the body includes upper lid Contracting component (11), the upper lid (10) and thickening apparatus (11) closely connect；The sample inlet (1) is located on lid (10), Sample export (2) and blank solution outlet (3) are located on thickening apparatus (11).
- 3. micro-fluidic syringe filter according to claim 2, it is characterised in that：The liquid storage pool (5), fluid channel (6) And Y types bifurcated flow-channel (7) is each provided on the end face of top lid (10) side of thickening apparatus (11)；The concentrate passage (8) Run through the thickening apparatus (11) with blank solution passage (9).
- 4. micro-fluidic syringe filter according to claim 2, it is characterised in that：Sealing gasket (12) is additionally provided with, it is described close Packing (12) is located between lid (10) and thickening apparatus (11).
- 5. micro-fluidic syringe filter according to claim 1, it is characterised in that：The cross section shape of the fluid channel (6) Shape is low depth-to-width ratio rectangle.
- 6. micro-fluidic syringe filter according to claim 1, it is characterised in that：The cross section chi of the fluid channel (6) The relation of particle size very little and to be concentrated is：0.07≤ap/Lc≤0.6Wherein, apFor the maximum gauge of concentrating particulate；Lc is the characteristic size of fluid channel (6).
- 7. micro-fluidic syringe filter according to claim 1, it is characterised in that：The sample inlet (1), sample export (2) standard luer fittings are used.
- 8. the application method of the micro-fluidic syringe filter according to claim 1-7 any one, it is characterised in that including Following step：(1) according to the filter of the size of object to be concentrated and scattered Sexual behavior mode appropriate size；(2) syringe of appropriate volume is chosen, draws sample liquid to be concentrated, and injector head is inserted into sample inlet 1；(3) pipeline that sample export 2 and blank solution outlet 3 are connected to conventional syringe syringe needle or other female Luers respectively connects Part, different liquids storage area is drained to respectively；(4) medical injection pump or hand push mode at the uniform velocity pushing syringe are used, until liquid has all pushed away in syringe；(5) liquid that sample export 2 is collected is loaded into syringe again, and repeats the above steps (1) to step (4), to enter One step lifts the concentration of sample liquid.
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| CN201710816147.1A CN107377024B (en) | 2017-09-11 | 2017-09-11 | Micro-fluidic syringe filter and its application method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108434551A (en) * | 2018-04-13 | 2018-08-24 | 山东中医药大学 | A kind of dropper with injection filtering function |
| CN109967150A (en) * | 2019-04-24 | 2019-07-05 | 河海大学常州校区 | It is a kind of for manipulating the inertia micro-fluidic chip of micro-nano granules |
| CN110841736A (en) * | 2019-12-20 | 2020-02-28 | 凡知医疗科技(江苏)有限公司 | Micro-fluidic sample introduction and filtration device |
| CN111330656A (en) * | 2020-03-03 | 2020-06-26 | 东南大学 | Micro-fluidic device for micro-particle suspension volume concentration |
| CN111735853A (en) * | 2020-06-16 | 2020-10-02 | 东南大学 | Integrated pre-sorting cell mechanical and electrical multi-parameter joint detection device |
| CN111744565A (en) * | 2020-05-26 | 2020-10-09 | 东南大学 | Microfluidic device and system for multi-channel parallel detection of cell deformability |
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| CN105214747A (en) * | 2015-11-11 | 2016-01-06 | 东南大学 | A kind of clip type micro-fluidic device and manufacture method |
| CN105642377A (en) * | 2016-01-28 | 2016-06-08 | 浙江大学 | Pump-driving-free micro-fluidic chip manufacturing method based on three-dimensional printing and product |
| CN106994369A (en) * | 2017-05-22 | 2017-08-01 | 东南大学 | Regulatable micro-fluidic integrated device of flux and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105214747A (en) * | 2015-11-11 | 2016-01-06 | 东南大学 | A kind of clip type micro-fluidic device and manufacture method |
| CN105642377A (en) * | 2016-01-28 | 2016-06-08 | 浙江大学 | Pump-driving-free micro-fluidic chip manufacturing method based on three-dimensional printing and product |
| CN106994369A (en) * | 2017-05-22 | 2017-08-01 | 东南大学 | Regulatable micro-fluidic integrated device of flux and preparation method thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108434551A (en) * | 2018-04-13 | 2018-08-24 | 山东中医药大学 | A kind of dropper with injection filtering function |
| CN109967150A (en) * | 2019-04-24 | 2019-07-05 | 河海大学常州校区 | It is a kind of for manipulating the inertia micro-fluidic chip of micro-nano granules |
| CN110841736A (en) * | 2019-12-20 | 2020-02-28 | 凡知医疗科技(江苏)有限公司 | Micro-fluidic sample introduction and filtration device |
| CN111330656A (en) * | 2020-03-03 | 2020-06-26 | 东南大学 | Micro-fluidic device for micro-particle suspension volume concentration |
| CN111744565A (en) * | 2020-05-26 | 2020-10-09 | 东南大学 | Microfluidic device and system for multi-channel parallel detection of cell deformability |
| CN111744565B (en) * | 2020-05-26 | 2022-03-08 | 东南大学 | Microfluidic device and system for multi-channel parallel detection of cell deformability |
| CN111735853A (en) * | 2020-06-16 | 2020-10-02 | 东南大学 | Integrated pre-sorting cell mechanical and electrical multi-parameter joint detection device |
| CN111735853B (en) * | 2020-06-16 | 2022-03-29 | 东南大学 | Integrated pre-sorting cell mechanical and electrical multi-parameter joint detection device |
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| Publication number | Publication date |
|---|---|
| CN107377024B (en) | 2019-06-21 |
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