CN107375374B - A kind of fermentation extraction method for the American ginseng extract that there is auxiliary to improve sleep effect - Google Patents
A kind of fermentation extraction method for the American ginseng extract that there is auxiliary to improve sleep effect Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of fermentation extraction method of the American ginseng extract or ginseng extract that there is auxiliary to improve sleep effect, comprise the following steps：(1) Feedstock treating：It is spare after crushing, expanding treatment using American Ginseng or ginseng as raw material；(2) one time fermentation：The American Ginseng after expanding treatment or ginseng are digested using cellulase, obtain the enzymolysis liquid of American Ginseng or ginseng, inactivation treatment, sieving for standby then are carried out to enzymolysis liquid；(3) secondary fermentation：Fermentation medium is prepared with enzymolysis liquid, carbon source and nitrogen source, is then inoculated with aspergillus oryzae bacterium solution, ferments and decomposes at 35 45 DEG C, obtain enzyme liquid；(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration, obtain the extract of American Ginseng or ginseng.The present invention passes through extraction American Ginseng or the ginseng of fermenting, small-molecule substance is converted into by the macromolecular substances such as absorbed ginsenoside are not easy in American Ginseng or ginseng, functional food containing GABA is produced by the secondary fermentation of American Ginseng or ginseng, is slept so as to aid in improving.
Description
Technical field
The present invention relates to the extracting method of a kind of American ginseng extract or ginseng extract, more particularly to one kind to have auxiliary
Improve the fermentation extraction method of American ginseng extract/ginseng extract of sleep effect.
Background technology
Sleep is the premise of human survival, and the mankind carry out dispelling fatigue by sleep, regain one's vigor；Protect brain, enhancing note
Recall；Improve immune, rehabilitation body, promote growth, anti-aging.Increasingly fierce and rhythm of life with social competition adds
It hurry up, sleep-disorder or and relevant disease of sleeping occur in more and more people.Show according to investigations, sleep-disorder occurs in adult
Ratio up to 35%, sleep-disorder occurs in the elderly 57% of more than 60 years old, is slept in children within 2~6 years old in some cities
Dormancy obstacle accounts for 27%.In addition pregnant woman sleeps incidence up to 75% in pregnant latter stage of being pregnent.Some researches show that extended sleep is less than 5
Hour be able to can be easier to suffer from hypertension, diabetes, obesity, can also induce melancholia.Especially middle-aged and elderly people's sleep-disorder can lure
Send out nighttime sleep arrhythmia cordis, sudden death, palsy, pulmonary hypertension, twitch and decrease of cognitive function etc..
Sleep-disorder refers to that sleep quality is abnormal or occurs some clinical symptoms, and such as sleep reduces or hypersomnia,
Sleep associated dyskinesias etc., wherein most commonly seen to have a sleepless night.Sleep-disorder becomes worldwide disease, international mental hygiene and
Neuscience foundation was set up in 2001 established sleep medicine annual March 21 in succession for sleep day, countries in the world, into
Hypnosphy meeting is stood, and clearly sleep science is a comprehensive subject, including clinical sleep medicine, Sleep hygiene, has slept
Environmentology, sleep engineering science etc..
Sleep state one shows as eyes and rotates rapidly, that is, (shallow sleep, REM are slept famous fast REM sleep
Sleep), at this moment brain neopallium is rested, but cerebral limbic system's (diencephalon, midbrain, pon, oblongata) is in waking state, is carried out
Intracerebral information processing, remembers the work such as fixed；And another stage is then directly referred to as non-fast REM sleep (deep sleep, HREM
Sleep), at this moment body is fully rested, immune to be improved, and is played a decisive role to health.The sleep of a whole night is different journeys
What the multiple alternate repetition of shallow sleep and deep sleep of degree carried out.
Dormant startup and maintenance are related with numerous transmitter substances and hormone (also referred to as sleep substance) in vivo.
The sleep substance understood has tens of kinds, for example known melatonin (melatonin), melatonin are human brains
A kind of amine bormones of " pineal body " secretion, is a kind of hormone in vivo for inducing natural sleep, also has lateral hypothalamic region synthesis
Small molecule nerve polypeptide orexin (Orexin) of the secretion with effect of ingesting, and central nervous system impression transmit material
γ-aminobutyric acid (GABA), oxidized form of glutathione (GSSG) are also a kind of sleep property material, it is by suppressing excitatory neuron
Transmitter glutamate plays sedation.Internal sleep substance status level determines sleep quality substantially as can be seen here.
γ-aminobutyric acid (GABA) is a kind of active skull cap components, is distributed widely in animal and plant body.Plant such as Macroptilium,
Join in the seed, rhizome and tissue fluid of category, Chinese herbal medicine etc. and all contain GABA.In animal body, GABA almost exists only in nerve
In tissue, the content in its mesencephalic tissue is about 0.1-0.6mg/ grams of tissue, and immunological investigation shows, the highest area of its concentration
Domain is black substance in brain.GABA mainly has following physiological activity：1. calm nerve, antianxiety.2. blood pressure lowering.3. improve sleep,
Improve memory.4. prevent and treat the neurological diseases such as epilepsy.5. the effect of removing toxic substances.
GABA be by Pidolidone (Glu) or derivatives thereof under glutamic acid decarboxylase enzyme effect, take off what α-carboxyl was formed,
Therefore the reduction of content of glutamic acid may be considered the synthesis of GABA.The synthetic method of GABA mainly has chemical synthesis and biology
Synthetic method, chemical synthesis GABA are needed using stronger solvents of corrosivity such as strong acid and highly basic, and reaction condition is violent, material toxicity
Greatly, it is expensive and there are more safety issue.Therefore biological synthesis process, biosynthesis mainly are used in actual production
Method is mainly by way of fermentation, and the fermentation method of early stage is mainly with Escherichia coli (Escherichia coli) fermenting and producing
Based on GABA.Due to the consideration of food-safe property, saccharomycete, lactic acid bacteria and the Aspergillus of glutamate decarboxylase are gradually screened
Etc. food security level microbe come the GABA that ferments.
Microbial fermentation is for raw material, through multiple beneficial with one or more fresh vegetables, fruit, mushroom, Chinese herbal medicine etc.
What bacterium fermented and produced, the micro- life of the feature containing nutritional ingredients such as abundant vitamin, enzyme, mineral matter and secondary metabolites
Thing fermented product.Enzyme microb not only saves nutriment intrinsic in fermentation raw material, and has many active ingredients
Substantial increase, and generate some new bioactive ingredients and biology enzyme.
The content of the invention
The object of the present invention is to provide a kind of American ginseng extract or ginseng extract that there is auxiliary to improve sleep effect
Fermentation extraction method.
The purpose of the present invention is what is be achieved through the following technical solutions：A kind of the West that there is auxiliary to improve sleep effect
The fermentation extraction method of conopsea extraction or ginseng extract, comprises the following steps：
(1) Feedstock treating：It is spare after crushing, expanding treatment using American Ginseng or ginseng as raw material；
(2) one time fermentation：The American Ginseng after expanding treatment or ginseng are digested using cellulase, obtain west
The enzymolysis liquid of American ginseng or ginseng, then carries out inactivation treatment, sieving for standby to enzymolysis liquid；
(3) secondary fermentation：Fermentation medium is prepared with enzymolysis liquid, carbon source and nitrogen source, is then inoculated with aspergillus oryzae bacterium solution,
Ferment and decompose at 35-45 DEG C, obtain ferment enzyme liquid；
(4) concentrate：The ferment enzyme liquid of gained is filtered, gained supernatant concentration, obtain the hair of American Ginseng or ginseng
Ferment extract.
American Ginseng and ginseng is belong to not kindred plant together, and the component contained by the two is largely identical, and main component is people
Join saponin(e.The compounds such as the ginsenoside contained in American Ginseng and ginseng are macromolecular compound, it is not easy to are absorbed.The present invention
By carrying out fermentation process to American Ginseng and ginseng, the macromolecular compounds such as ginsenoside are resolved into micromolecular compound, and
The functional components containing GABA are produced in secondary fermentation, so as to have effects that auxiliary improves sleep.
In the step (1), the American Ginseng refers not only to American ginseng root or refers to Folium Panacis Quinquefolii, American Ginseng stem
Or other positions of American Ginseng that American ginseng root palpus etc. very utilizes less at present.The ginseng refers not only to ginseng or refers to
Other positions of ginseng that folium panacis japonici cum caule, ginseng stem or ginseng palpus etc. very utilize less at present.
In the step (1), 115-130 DEG C of swelling temperature after American Ginseng or ginseng crushing.
In the step (2), the temperature of enzymolysis：When 45-55 DEG C of constant temperature, enzymolysis time are small not less than 5.
In the step (2), in terms of American Ginseng or ginseng gross mass, the cellulase addition is 55-65u/g.
In the step (2), 80-90 DEG C of inactivation temperature, inactivation time 25-35 minutes.
In the step (3), in fermentation medium, the mass fraction of enzymolysis liquid, carbon source and nitrogen source is as follows：
Enzymolysis liquid 10-30%, carbon source 40-60%, nitrogen source 20-40%.
In the step (3), the inoculum concentration of aspergillus oryzae nutrient solution is the 8-12% of fermentation medium volume.
As one embodiment of the present of invention, the carbon source includes but not limited to wholewheat flour, wheat degreasing plumule and small
Combination more than one or both of wheat germ.The nitrogen source includes but not limited to casein, and the casein is included but not
It is limited to tryptone.
As one embodiment of the present of invention, aspergillus oryzae nutrient solution obtains in the following ways in the step (3)：
Using flour as carbon source, casein prepares culture medium for nitrogen source, and aspergillus oryzae bacterium solution is inoculated in the culture medium,
When shaking table culture 5-8 is small at 30-40 DEG C, you can obtain aspergillus oryzae nutrient solution.
In the step (4), supernatant concentration to 1/10-1/100, up to American Ginseng or the extractive from fermentative of ginseng.
It is dry present invention additionally comprises step (6), and the extractive from fermentative of American Ginseng or ginseng is freeze-dried, obtain
Powdery product, easy to preserve.
The present invention has the advantages that compared with prior art：
The present invention will be not easy absorbed ginseng by the technology of ferment extraction American Ginseng or ginseng in American Ginseng or ginseng
The macromolecular compounds such as saponin(e are converted into micromolecular compound, are produced by the secondary fermentation of American Ginseng or ginseng and contain GABA's
Functional components, sleep so as to aid in improving.
Brief description of the drawings
Fig. 1 be the embodiment 1 of four different sampling amounts American ginseng extract under differential responses time point glutamic acid contain
Measure the curve map of slip.
Fig. 2 be the embodiment 2 of four different sampling amounts American ginseng extract under differential responses time point glutamic acid contain
Measure the curve map of slip.
Fig. 3 be the embodiment 3 of four different sampling amounts American ginseng extract under differential responses time point glutamic acid contain
Measure the curve map of slip.
Embodiment
Below in conjunction with attached drawing, further to the preferred embodiment of the present invention, it is described in detail.Described in following embodiments
American Ginseng or ginseng refer not only to American Ginseng/ginseng or refer to American Ginseng/folium panacis japonici cum caule, American Ginseng/ginseng stem or the West
Other positions of American Ginseng/ginseng that ginseng/ginseng palpus etc. very utilizes less at present.
Embodiment 1
(1) Feedstock treating：Using American Ginseng as raw material, crush, it is spare after 120 DEG C of expanding treatments.
(2) one time fermentation：In American Ginseng after puffing, 60u/g (in terms of American Ginseng gross mass) cellulase is added, then
It is heated to 50 DEG C and keeps constant temperature, when 150r/min stirring decomposing and fermentings 5 is small.After fermentation, 16 mesh of filter centrifugal filters,
Obtain American Ginseng enzymolysis liquid.American Ginseng enzymolysis liquid is heated to 90 DEG C, inactivates 30min, it is spare.
(3) secondary fermentation：Using flour as carbon source, casein prepares culture medium for nitrogen source, by 1:100 connect aspergillus oryzae bacterium solution
Kind is in the culture medium, when shaking table culture 6 is small at 35 DEG C, you can obtains aspergillus oryzae bacterium solution.Using following mass percent
Raw material prepare fermentation medium：American Ginseng enzymolysis liquid 20%, wholewheat flour 50%, tryptone 30%.Then by fermentation training
10% inoculation aspergillus oryzae bacterium solution of matrix product is supported, is placed in fermentation tank, at 40 DEG C, lower fermentation is stirred under vacuum and decomposes, mixing speed
For 150r/min, ferment 3h, obtains enzyme liquid.
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration to 1/10-1/100, obtain American Ginseng
Extractive from fermentative.
(5) it is dry：American Ginseng extractive from fermentative is prepared into powdery product, easy to preserve by freeze-drying.
Embodiment 2
(1) Feedstock treating：Using American Ginseng as raw material, crush, it is spare after 120 DEG C of expanding treatments.
(2) one time fermentation：In American Ginseng after puffing, 60u/g (in terms of American Ginseng gross mass) cellulase is added, then
It is heated to 50 DEG C and keeps constant temperature, when 150r/min stirring decomposing and fermentings 5 is small.After fermentation, 16 mesh of filter centrifugal filters,
Obtain American Ginseng enzymolysis liquid.American Ginseng enzymolysis liquid is heated to 90 DEG C, inactivates 30min, it is spare.
(3) secondary fermentation：Using flour as carbon source, casein prepares culture medium for nitrogen source, by 1:100 connect aspergillus oryzae bacterium solution
Kind is in the culture medium, when shaking table culture 6 is small at 35 DEG C, you can obtains aspergillus oryzae bacterium solution.Using following mass percent
Raw material prepare fermentation medium：American Ginseng enzymolysis liquid 20%, wheat embryo 50%, tryptone 30%.Then by fermentation training
10% inoculation aspergillus oryzae bacterium solution of matrix product is supported, is placed in fermentation tank, at 40 DEG C, lower fermentation is stirred under vacuum and decomposes, mixing speed
For 150r/min, ferment 4h, obtains enzyme liquid.
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration to 1/10-1/100, obtain American Ginseng
Extractive from fermentative.
(5) it is dry：American Ginseng extractive from fermentative is prepared into powdery product, easy to preserve by freeze-drying.
Embodiment 3
(1) Feedstock treating：Using American Ginseng as raw material, crush, it is spare after 120 DEG C of expanding treatments.
(2) one time fermentation：In American Ginseng after puffing, 60u/g (in terms of American Ginseng gross mass) cellulase is added, then
It is heated to 50 DEG C and keeps constant temperature, when 150r/min stirring decomposing and fermentings 5 is small.After fermentation, 16 mesh of filter centrifugal filters,
Obtain American Ginseng enzymolysis liquid.American Ginseng enzymolysis liquid is heated to 90 DEG C, inactivates 30min, it is spare.
(3) secondary fermentation：Using flour as carbon source, casein prepares culture medium for nitrogen source, by 1:100 connect aspergillus oryzae bacterium solution
Kind is in the culture medium, when shaking table culture 6 is small at 35 DEG C, you can obtains aspergillus oryzae bacterium solution.Using following mass percent
Raw material prepare fermentation medium：American Ginseng enzymolysis liquid 20%, wheat degreasing plumule 50%, tryptone 30%.Then by hair
10% inoculation aspergillus oryzae bacterium solution of ferment culture volume, is placed in fermentation tank, at 40 DEG C, is stirred under vacuum lower fermentation and decomposes, stirring
Speed is 150r/min, and ferment 2h, obtains enzyme liquid.
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration to 1/10-1/100, obtain American Ginseng
Extractive from fermentative.
(5) it is dry：American Ginseng extractive from fermentative is prepared into powdery product, easy to preserve by freeze-drying.
Embodiment 4
(1) Feedstock treating：Using ginseng as raw material, crush, it is spare after 120 DEG C of expanding treatments.
(2) one time fermentation：In ginseng after puffing, 55u/g (in terms of ginseng gross mass) cellulase is added, is then heated
To 45 DEG C and constant temperature is kept, when 150r/min stirring decomposing and fermentings 5 are small.After fermentation, 16 mesh of filter centrifugal filters, and obtains
Obtain ginseng enzymolysis liquid.Ginseng enzymolysis liquid is heated to 90 DEG C, inactivates 30min, it is spare.
(3) secondary fermentation：Using flour as carbon source, casein prepares culture medium for nitrogen source, by 1:100 connect aspergillus oryzae bacterium solution
Kind is in the culture medium, when shaking table culture 8 is small at 35 DEG C, you can obtains aspergillus oryzae bacterium solution.Using following mass percent
Raw material prepare fermentation medium：Ginseng enzymolysis liquid 20%, wholewheat flour 60%, tryptone 20%.Then fermented and cultured is pressed
10% inoculation aspergillus oryzae bacterium solution of matrix product, is placed in fermentation tank, at 35 DEG C, is stirred under vacuum lower fermentation and decomposes, mixing speed is
150r/min, ferment 1h, obtains enzyme liquid.
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration to 1/10-1/100, obtain ginseng hair
Ferment extract.
(5) it is dry：Fermented ginseng extract is prepared into powdery product, easy to preserve by freeze-drying.
Embodiment 5
(1) Feedstock treating：Using ginseng as raw material, crush, it is spare after 120 DEG C of expanding treatments.
(2) one time fermentation：In ginseng after puffing, 55u/g (in terms of ginseng gross mass) cellulase is added, is then heated
To 45 DEG C and constant temperature is kept, when 150r/min stirring decomposing and fermentings 5 are small.After fermentation, 16 mesh of filter centrifugal filters, and obtains
Obtain ginseng enzymolysis liquid.Ginseng enzymolysis liquid is heated to 90 DEG C, inactivates 30min, it is spare.
(3) secondary fermentation：Using flour as carbon source, casein prepares culture medium for nitrogen source, by 1:100 connect aspergillus oryzae bacterium solution
Kind is in the culture medium, when shaking table culture 8 is small at 35 DEG C, you can obtains aspergillus oryzae bacterium solution.Using following mass percent
Raw material prepare fermentation medium：Ginseng enzymolysis liquid 30%, wheat embryo 40%, tryptone 30%.Then fermented and cultured is pressed
10% inoculation aspergillus oryzae bacterium solution of matrix product, is placed in fermentation tank, at 35 DEG C, is stirred under vacuum lower fermentation and decomposes, mixing speed is
150r/min, ferment 2.5h, obtains enzyme liquid.
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration to 1/10-1/100, obtain ginseng hair
Ferment extract.
(5) it is dry：Fermented ginseng extract is prepared into powdery product, easy to preserve by freeze-drying.
Embodiment 6
(1) Feedstock treating：Using American Ginseng as raw material, crush, it is spare after 120 DEG C of expanding treatments.
(2) one time fermentation：In American Ginseng after puffing, 55u/g (in terms of American Ginseng gross mass) cellulase is added, then
It is heated to 45 DEG C and keeps constant temperature, when 150r/min stirring decomposing and fermentings 5 is small.After fermentation, 16 mesh of filter centrifugal filters,
Obtain American Ginseng enzymolysis liquid.American Ginseng enzymolysis liquid is heated to 90 DEG C, inactivates 30min, it is spare.
(3) secondary fermentation：Using flour as carbon source, casein prepares culture medium for nitrogen source, by 1:100 connect aspergillus oryzae bacterium solution
Kind is in the culture medium, when shaking table culture 8 is small at 35 DEG C, you can obtains aspergillus oryzae bacterium solution.Using following mass percent
Raw material prepare fermentation medium：American Ginseng enzymolysis liquid 10%, wheat degreasing plumule 55%, tryptone 35%.Then by hair
10% inoculation aspergillus oryzae bacterium solution of ferment culture volume, is placed in fermentation tank, at 35 DEG C, is stirred under vacuum lower fermentation and decomposes, stirring
Speed is 150r/min, and ferment 3.5h, obtains enzyme liquid.
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration to 1/10-1/100, obtain American Ginseng
Extractive from fermentative.
(5) it is dry：American Ginseng extractive from fermentative is prepared into powdery product, easy to preserve by freeze-drying.
Experimental example
GABA be by Pidolidone (Glu) or derivatives thereof under glutamic acid decarboxylase enzyme effect, take off what α-carboxyl was formed,
Therefore the reduction of content of glutamic acid may be considered the synthesis of GABA.Sentenced by the slip of content of glutamic acid after measure fermentation
The generation of disconnected GABA.
Content of glutamic acid measures：Content detection is carried out using glutamic acid detection kit, it is public purchased from U.S. eBioscience
Department.
1 principle：Using kit provide special extract extract, then developed the color with color developing agent, after colour developing
It is measured under 570nm.
2. the preparation of sample：It is detected using distilled water dilute sample.
3. detection process：
Wavelength：(Hg)492nm
Cuvette：1.00cm light path
Temperature：20-25℃
Final volume：3.030ml
Compare air (without cuvette in light path), water or blank reading.
Sample solution：0.4-14 μ L Pidolidone/time (sample volume 0.200-2.000ml)
Result of calculation：Δ A=(A2-A1)Sample- (A2-A1)BlankContent of glutamic acid c=0.1122 in every liter of sample ×
If Δ A sample volumes are up to 2.000ml, lowest detection is limited to 0.2mg/L.
Experimental example 1：
Content of glutamic acid (mg/mL) measurement result of the American ginseng extract of embodiment 1 is referring to Fig. 1.Can from Fig. 1
Go out, content of glutamic acid has different degrees of decline under conditions of 4 reaction time, 4 sampling amounts, shows the life of GABA
Into.
Experimental example 2：
Content of glutamic acid (mg/mL) measurement result of the American ginseng extract of embodiment 2 is referring to Fig. 2.Can from Fig. 2
Go out, content of glutamic acid has different degrees of decline wherein 0.4ml ferment amount under conditions of 3 reaction time, 3 sampling amounts
Content of glutamic acid increases after when 3 is small.When showing except sampling amount increase, the slip of content of glutamic acid increases, and GABA generations increase
Add.
Experimental example 3：
The measurement result of the content of glutamic acid slip of the American ginseng extract of embodiment 3 is referring to Fig. 3.Can from Fig. 3
Go out, in reaction different time recklessly, content of glutamic acid is reduced the ferment of different sampling amounts, paddy ammonia when wherein sampling amount is 2mL
The slip of acid content is maximum, illustrates that the growing amount of the GABA under this sampling amount is more.
Claims (10)
1. a kind of fermentation extraction method for the American ginseng extract that there is auxiliary to improve sleep effect, it is characterized in that, including it is following
Step：
(1) Feedstock treating：It is spare after crushing, expanding treatment using American Ginseng as raw material；
(2) one time fermentation：The American Ginseng after expanding treatment is digested using cellulase, obtains American Ginseng enzymolysis
Liquid, then carries out inactivation treatment, sieving for standby to enzymolysis liquid；
(3) secondary fermentation：Fermentation medium is prepared with enzymolysis liquid, carbon source and nitrogen source, aspergillus oryzae bacterium solution is then inoculated with, in 35-45
Ferment and decompose at DEG C, obtain enzyme liquid；
(4) concentrate：The enzyme liquid of gained is filtered, gained supernatant concentration, obtain American Ginseng extractive from fermentative.
2. the fermentation extraction method of the American ginseng extract according to claim 1 that there is auxiliary to improve sleep effect, its
It is characterized in, in the step (1), the American Ginseng is American ginseng root, Folium Panacis Quinquefolii, American Ginseng stem or American ginseng root palpus.
3. the fermentation extraction method of the American ginseng extract according to claim 1 that there is auxiliary to improve sleep effect, its
It is characterized in, in the step (1), 115-130 DEG C of swelling temperature after American Ginseng crushing.
4. the fermentation extraction method of the American ginseng extract according to claim 1 that there is auxiliary to improve sleep effect, its
It is characterized in, in the step (2), in terms of American Ginseng gross mass, the cellulase addition is 55-65u/g；The enzymolysis
Temperature：When 45-55 DEG C of constant temperature, enzymolysis time are small not less than 5；In the step (2), 80-90 DEG C of inactivation temperature, inactivation time
25-35 minutes.
5. the fermentation extraction method of the American ginseng extract according to claim 1 that there is auxiliary to improve sleep effect, its
It is characterized in, in the step (3), in fermentation medium, the mass fraction of enzymolysis liquid, carbon source and nitrogen source is as follows：Enzymolysis liquid 10-
30%, carbon source 40-60%, nitrogen source 20-40%.
6. the fermentation extraction method of the American ginseng extract according to claim 5 that there is auxiliary to improve sleep effect, its
It is characterized in, the carbon source includes but not limited to more than one or both of wholewheat flour, wheat degreasing plumule and wheat embryo
Combination；The nitrogen source includes but not limited to casein；The casein includes but not limited to tryptone.
7. the fermentation that having auxiliary according to claim 1-6 any one of them improves the American ginseng extract that sleep acts on is extracted
Method, it is characterized in that, in the step (3), the inoculum concentration of aspergillus oryzae nutrient solution is the 8-12% of fermentation medium volume.
8. the fermentation extraction method of the American ginseng extract according to claim 7 that there is auxiliary to improve sleep effect, its
It is characterized in, aspergillus oryzae nutrient solution obtains in the following ways in the step (3)：
Using flour as carbon source, casein prepares culture medium for nitrogen source, aspergillus oryzae bacterium solution is inoculated in the culture medium, in 30-
When shaking table culture 5-8 is small at 40 DEG C, you can obtain aspergillus oryzae nutrient solution.
9. the fermentation extraction method of the American ginseng extract according to claim 1 that there is auxiliary to improve sleep effect, its
It is characterized in, in the step (4), supernatant concentration to 1/10-1/100, up to the extractive from fermentative of American Ginseng.
10. the fermentation extraction method of the American Ginseng according to claim 1 that there is auxiliary to improve sleep effect, its feature
It is to further include step (5) drying, i.e., American Ginseng extractive from fermentative is freeze-dried, obtains powdery product.
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Denomination of invention: A Fermentation Extraction Method of American Ginseng Extract with Assistive Sleep Improvement Effect Effective date of registration: 20231113 Granted publication date: 20180420 Pledgee: Tonghua Branch of China Construction Bank Corp. Pledgor: Dongfanghong American ginseng Pharmaceutical (Tonghua) Co.,Ltd. Registration number: Y2023220000124 |
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