CN107373024A - Animal feed additive and its preparation method and application - Google Patents
Animal feed additive and its preparation method and application Download PDFInfo
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- CN107373024A CN107373024A CN201710471634.9A CN201710471634A CN107373024A CN 107373024 A CN107373024 A CN 107373024A CN 201710471634 A CN201710471634 A CN 201710471634A CN 107373024 A CN107373024 A CN 107373024A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The present invention relates to cultivation field, more particularly to animal feed additive and its preparation method and application, animal feed additive is mixed by following components, Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, bacillus amyloliquefaciens, bacillus subtilis, lactobacillus acidophilus, pichia farinose, Pichia membranaefaciens, moral gram yeast, Brussels moral kirschner yeast.The present invention can improve animal survival rate, accelerate animal growth speed, improve meat quality, do not interfere with human health.
Description
Technical field
The present invention relates to cultivation field, more particularly to animal feed additive and its preparation method and application.
Background technology
Feed addictive refers to a small amount of or micro substance added during Feed Manufacturing processing, use, in feed
Dosage is seldom but effect is notable.Feed addictive is the raw material that modern feed industry necessarily uses, to strengthening basal feed nutrition
Value, breeding performonce fo animals are improved, ensure animal health, saving feed cost, improvement livestock products quality etc. has obvious
Effect.
Prevailing with scientific cultivation, cultivation of the people for animal has more deep awareness and understanding, how to carry
The survival rate of high animal, the generation of disease is reduced, accelerate its growth rate and improve meat quality, be that numerous cultivation dealers are urgent
Inquisitive problem.
To prevent and treat Animal diseases, animal health is often ensured using excessive antibiotic as feed addictive, but
The use of excessive antibiotic, on the one hand, cause the quality decline of animal meat product and its product, second aspect, antibiotic resistance
Enhancing, cause the appearance of a large amount of drug-fast bacterias, strain, the third aspect, cause human health also by anti-by the transmission of food chain
Having a strong impact on for raw Plain Questions topic, great infringement is brought to public health and food security, with people pursue " high-quality,
Low medicine is residual, green non-pollution " food consumption trend disagree.
To accelerate animal growth speed, hormone is often used to have an impact to animal, further influence as feed addictive
The health of people.
The content of the invention
Animal survival rate can be improved, accelerates animal growth speed, improve meat to solve the above problems, the present invention provides one kind
Matter quality, the animal feed additive for not interfering with human health and its preparation method and application.
The technical solution adopted in the present invention is：Animal feed additive, mixed by following components, lactic acid sheet ball
Bacterium, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, bacillus amyloliquefaciens, bacillus subtilis, lactobacillus acidophilus,
Pichia farinose, Pichia membranaefaciens, moral gram yeast, Brussels moral kirschner yeast.
To being further improved to for above-mentioned technical proposal, the concentration of the Pediococcus acidilactici is 7.0x 107 cfu/gm-
9.0x 107Cfu/gm, the concentration of the Pediococcus pentosaceus is 6.0x 107cfu/gm-8.0x 107Cfu/gm, the goldbeater's skin are bright
The concentration of beading bacterium is 2.0x 106cfu/gm-4.0x 106Cfu/gm, the concentration of the Lactobacillus plantarum is 3.0x 107cfu/
gm-5.0x 107Cfu/gm, the concentration of the bacillus amyloliquefaciens is 4.0x 106cfu/gm-6.0x 106Cfu/gm, institute
The concentration for stating bacillus subtilis is 3.0x 107cfu/gm-5.0 x 107Cfu/gm, the concentration of the lactobacillus acidophilus are
3.0x 106cfu/gm-5.0x 106Cfu/gm, the concentration of the pichia farinose is 5.0x 107cfu/gm-7.0x
107Cfu/gm, the concentration of the Pichia membranaefaciens is 8.5x 106cfu/gm-9.5x 106Cfu/gm, the moral gram yeast
Concentration is 7.0x 106cfu/gm-9.0x 106Cfu/gm, the concentration of Brussels moral kirschner yeast is 3.0x 107cfu/
gm-5.0x 107cfu/gm。
The preparation method of animal feed additive, comprises the following steps, and a, prepares fluid nutrient medium and strain, b, by bacterium
Kind be seeded in fluid nutrient medium and carry out liquid fermentation, obtain zymotic fluid, c, take zymotic fluid in step b, into zymotic fluid plus
Enter dry material, carry out solid fermentation, obtain animal feed additive, d, animal feed additive is subjected to packaging storage.
To being further improved to for above-mentioned technical proposal, in step a, fluid nutrient medium component is included for dregs of beans, oil, shallow lake
Powder, whole milk powder, NaCl, ZnSO4, CuSO4, MgSO4, MnSO4, FeSO4, CaSO4, industrial water；Strain is lactic acid sheet ball
Bacterium, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, bacillus amyloliquefaciens, bacillus subtilis, lactobacillus acidophilus,
Pichia farinose, Pichia membranaefaciens, moral gram yeast, the mixture of Brussels moral kirschner yeast.
To being further improved to for above-mentioned technical proposal, in step b, when preparing fluid nutrient medium, strain is with Liquid Culture
Based raw material adds together, and temperature is 24 DEG C -40 DEG C in seeded process, to be stirred in seeded process, make strain and Liquid Culture
Base is well mixed.
To being further improved to for above-mentioned technical proposal, in step b, during liquid fermentation, fermentation temperature is 41 ± 1 DEG C, control
System fermentation ventilation ratio is 0.3v/v.m -1.3v/v.m, and speed of agitator 150rpm--170rpm, fermentation tank inner canister pressure is zero, fermentation
Incandescent lamp is installed to carry out illumination to fermentation tank inside in tank top.
To being further improved to for above-mentioned technical proposal, in step b, during liquid fermentation, fermentation process mid-early stage throughput
More than later stage throughput, pH is less than 5.0 in fermentation tank, treats that bacterium amount reaches 5 × 10 in fermentation tank8~9After fermentation is completed, liquid hair
The ferment cycle is 42h-48h.
To being further improved to for above-mentioned technical proposal, in step c, the dry material of addition is zymotic fluid, rice bran, the chaff dregs of rice,
Dregs of beans, salt, calcium carbonate, magnesium carbonate.
To being further improved to for above-mentioned technical proposal, in step c, during solid fermentation, yeasting temperature is 40 DEG C -44
DEG C, in solid fermentation process, stirring is carried out to fermentation reaction heap every 48h, control fermentation reaction heap central temperature is less than 55 DEG C.
The application of animal feed additive, animal feed additive is mixed in animal feed and forms mixture, then is used
Mixture letting animals feed, dosage are that feed per ton adds 500g animal feed additives.
Beneficial effects of the present invention are：
1st, on the one hand, the present invention uses 7 kinds of probiotics and 4 primary yeasts as additive, will not without hormone and antibiotic
Secondary pollution is produced, does not interfere with human health；Second aspect, the present invention in microorganism energy decomposing bacteria element and enzyme, can kill
Pathogen in dead animal body, to improve animal survival rate；The third aspect, the present invention in microorganism can be with the nutrition in feed
Material is raw material, is broken down into the material more conducively absorbed, improves the absorbability of animal, accelerates animal growth speed, carries
High meat quality, meet the food consumption trend of " high-quality, low medicine are residual, green non-pollution " of people's pursuit；Fourth aspect, this
Microorganism in the 11 of invention use, is 7 kinds of probiotics and 4 primary yeasts, this 11 kinds of microorganism groups, will not into a syntaxial system
Mutually suppress other side growth, can be produced in the presence of 7 kinds of probiotics and 4 primary yeasts are common on the contrary different products such as lactic acid,
Acetic acid, pediocin, nisin, killer toxin etc., a kind of metabolite of microorganism can be used as enzyme, trigger other micro-
The metabolic response of biology, so as to promote whole antimicrobial composition additive to produce more to have nutriment decomposition and right
Pathogen has the metabolite of bactericidal action, further improves animal survival rate, accelerates animal growth speed, improves meat product
Matter.5th aspect, because this 11 kinds of bacterium have the function of decomposing organic matter, can reduce the smell of animal metabolism product, be advantageous to
Environmental protection.
The operation principle of animal feed additive of the present invention is：
Pediococcus acidilactici, latin name：Pediococcus acidilactici, it is Pediococcus, Pediococcus acidilactici kind.One
Aspect, there is production acid, adjust gastrointestinal bacterial flora, maintain the function of intestinal microecology balance, increase gut epithelium endolymph cell
Quantity and goblet cell quantity, the generation of animal intestines and stomach tract disease is prevented, improve animal survival rate.Second aspect, lactic acid sheet ball
The bacteriocin of bacterium can suppress the growth of the harmful bacterias such as enteropathogenic E. Coli, salmonella, staphylococcus, streptococcus, further
Improve animal survival rate.The third aspect, the lysate of cell membrane can induce the release of various immune regulators to carry out stimulating animal
Immune system and play its immunologic function, improve animal to the immunocompetence of pathogen, further improve animal survival rate.4th
Aspect, it is sensitive to protease, promote animal to digest and assimilate protein, improve animal meat.
Pediococcus pentosaceus, latin name：Pediococcus pentosaceus, are Pediococcus, Pediococcus pentosaceus kind, penta
Bacteriocin caused by sugar-tablet coccus (Pediococcus pentosaceous) can suppress gram-positive pathogen, such as gold
Staphylococcus aureus, prevent pathogen from being damaged to animal, improve animal survival rate.
Leuconostoc mesenteroides, latin name：Leuconostoc mesenteroides, it is the Leuconostoc in lactic acid bacteria
Important strain.Leuconostoc mesenteroides energy fermenting carbohydrate produces a variety of acid and alcohol, has high acid ability, oxidation resistance and short of money
The abilities such as anti-pathogenic bacteria, gastrointestinal health of animals is maintained, improve animal survival rate.
Lactobacillus plantarum, latin name：Lactobacillus plantarum, it is one kind of lactic acid bacteria, in reproductive process
The energy distinctive lactobacillin of output, lactobacillin is a kind of material of the protein component with antibacterial action, has suppression
Bacterium acts on, and can improve animal survival rate.
Bacillus amyloliquefaciens, latin name：Bacillus amyloliquefaciens, bacillus subtilis Pseudomonas, a side
Face, a variety of antibacterial substances, including polypeptide, lipopeptid class and Antagonistic protein class etc. can be produced, be advantageous to improve animal survival rate.Its
In, Antagonistic protein can act on the cell membrane of disease fungus, make the membrane permeability of disease fungus increase, so as to suppress its growth,
The pathogen in animal body is killed, further improves animal survival rate.Second aspect, can reduce the animal endo enteritis factor and
Anti-inflammatory factors, Immune Function In Animals are improved, animal intestinal tract health, pathogen is substantially reduced, so as to reduce inflammatory reaction, have
Beneficial to animal health, animal survival rate is further improved.
Bacillus subtilis, latin name：Bacillus subtilis, on the one hand, bacillus subtilis thalli growth process
In caused subtilin, polymyxins, nystatin, gramicidins isoreactivity material, these active materials to pathogenic bacteria or
The conditioned pathogen of autogenous infection has obvious inhibitory action, improves the immunocompetence of animal, kills pathogen, improves animal
Survival rate；Second aspect, bacillus subtilis are the probiotics of animal, and itself has very strong enzymatic productivity, disappears rich in a variety of
Change enzyme, and there is complete excretory system, produced digestive ferment can be secreted into extracellular.Bacillus subtilis is added in feed
Bacterium can destroy plant cell wall construction, decomposition of cellulose enzyme, and discharge more thick protein, participate in animal alimentary canal
" enzyme pond ", promote animal to digest and assimilate nutrients, accelerate animal growth speed, improve meat quality.
Lactobacillus acidophilus, latin name：Lactobacillus acidophilus, there is antagonism to pathogenic microorganisms.
Lactobacillus acidophilus can secrete antibiotin class material (acidolin (acidolin), bacillus acidophilus plain (acidophilin),
Lactein (1aetocidon)), to antagonism caused by pathogenic entero becteria, intestinal health is kept, it is anti-not adding completely
In the case of raw element, the diarrhoea of pig can be preferably controlled, improves animal survival rate.
Pichia farinose, latin name：Pichia farinose, Pichia membranaefaciens, latin name：Pichia
Membranaefaciens, moral gram yeast, latin name：Dekkera anomala, Brussels moral kirschner yeast, latin name：
Dekkerabruxellensis.On the one hand, these yeast can effectively decompose various carbohydrate, sugar, starch and various albumen
Matter amino acid, the nutriment needed for propagation is provided for other probiotics.Second aspect, saccharomycete can secrete a variety of enzymes, such as
Cellulase, hemicellulase, zytase, amylase, pectase and ligninase etc., can be complicated effectively in degrading plant feed
Carbohydrate, small-molecule substance is degraded to, is advantageous to the absorption of animal, so as to improve the absorbability of animal, accelerated
Animal growth speed.
2nd, animal feed additive of the invention, the concentration of Pediococcus acidilactici is 7.0x 107cfu/gm-9.0x 107
Cfu/gm, preferably 8.0x 107Cfu/gm, the concentration of Pediococcus pentosaceus is 6.0x 107cfu/gm-8.0x 107Cfu/gm,
Preferably 7.0x 107The concentration of Leuconostoc mesenteroides described in cfu/gm is 2.0x 106cfu/gm-4.0x 106Cfu/gm, preferably
For 3.0x 106Cfu/gm, the concentration of the Lactobacillus plantarum is 3.0x 107cfu/gm-5.0 x 107Cfu/gm, it is preferably
4.0x 107Cfu/gm, the concentration of the bacillus amyloliquefaciens is 4.0x 106 cfu/gm-6.0x 106Cfu/gm, it is preferably
5.0x 106Cfu/gm, the concentration of the bacillus subtilis is 3.0 x 107cfu/gm-5.0x 107Cfu/gm, it is preferably
4.0x 107Cfu/gm, the concentration of the lactobacillus acidophilus is 3.0x 106cfu/gm-5.0x 106Cfu/gm, preferably 4.0x
106Cfu/gm, the concentration of the pichia farinose is 5.0x 107cfu/gm-7.0x 107Cfu/gm, preferably 6.0x
107Cfu/gm, the concentration of the Pichia membranaefaciens is 8.5x 106cfu/gm-9.5x 106Cfu/gm, preferably 9.0x
106Cfu/gm, the concentration of the moral gram yeast is 7.0x 106cfu/gm-9.0x 106Cfu/gm, preferably 8.0x 106cfu/
Gm, the concentration of Brussels moral kirschner yeast is 3.0x 107cfu/gm-5.0x 107Cfu/gm, preferably 4.0x 107
cfu/gm.In the formula of the present invention, Pediococcus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum, bacillus subtilis, powdery are finished red
Yeast, the concentration of Brussels moral kirschner yeast are higher, and bacteriostasis and promotion animal growth effect are more notable.
3rd, the preparation method of animal feed additive, fluid nutrient medium component include for dregs of beans, oil, starch, whole milk powder,
NaCl、ZnSO4, CuSO4, MgSO4, MnSO4, FeSO4, CaSO4, industrial water；Each raw material is natural material or pure reagent, is pacified
Full health, it is pollution-free.
4th, when the preparation method of animal feed additive prepares fluid nutrient medium, strain adds with liquid medium starting material
Enter, and be stirred continuously so that strain can be well mixed with fluid nutrient medium, and temperature is 24 DEG C -40 DEG C in seeded process, prevents from connecing
Kind temperature is too high causes bacteria inactivation rate, or temperature is too low causes microbial metabolism degree low.
5th, the preparation method of animal feed additive, during liquid fermentation, fermentation temperature is 41 ± 1 DEG C, control fermentation ventilation
Than for 0.3v/v.m -1.3v/v.m, speed of agitator 150rpm--170rpm, fermentation tank inner canister pressure is zero, installed at the top of fermentation tank
Incandescent lamp is with to the progress illumination of fermentation tank inside.The control of temperature, it is to prevent temperature is too high from inactivate strain, prevents that temperature is too low
Make strain bio activity relatively low, when temperature is 41 ± 1 DEG C, now the horizontal highest of strain biochemical reaction, strain fermentation react most
Strongly.In fermentation process, it is stirred continuously so that the filtrated air being passed through can be uniformly distributed, and improve the dissolved oxygen amount in fermentation tank.Hair
Illumination is carried out during ferment, the fermenting speed of strain can be improved.
6th, the preparation method of animal feed additive, during liquid fermentation, because early stage is mainly based on aerobic fermentation, the later stage
Mainly based on anaerobic fermentation, therefore earlier fermentation throughput is more than later stage throughput, can improve overall fermenting speed；Due to 11
In kind microorganism, have a liking for based on acids microorganism, therefore it is less than 5.0 to control pH in fermentation tank, maintains the acidity in fermentation tank
Environment, it is more beneficial for microbial fermentation；Treat that bacterium amount reaches 5 × 10 in fermentation tank8~9After fermentation is completed, and the liquid fermentation cycle is
42h-48h。
7th, the preparation method of animal feed additive, during solid fermentation, yeasting temperature is 40 DEG C -44 DEG C, solid hair
During ferment, stirring is carried out to fermentation reaction heap every 48h, control fermentation reaction heap central temperature is less than 55 DEG C.
8th, using the application of animal feed additive of the invention, coordinate animal feed to carry out letting animals feed, animal can be improved
To the absorption rate of feed, various pathogens are played with bacteriostasis, improves Immune Function In Animals, maintains animal gastrointestinal tract to be good for
Health, be advantageous to improve animal survival rate, accelerate animal growth speed, improve meat quality, and due to without each parahormone and
Antibiotic, human health is not interfered with；When dosage is that feed per ton adds 500g animal feed additives, now using effect
Well, use cost is low, and overall economic efficiency is best.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1：The preparation of animal feed additive
A, fluid nutrient medium and strain are prepared.
The composition of fluid nutrient medium is as shown in table 1.
Wherein, strain is Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, solution starch gemma bar
Bacterium, bacillus subtilis, lactobacillus acidophilus, pichia farinose, Pichia membranaefaciens, moral gram yeast, Brussels moral kirschner
The mixture of yeast.Wherein, Pediococcus acidilactici is to be purchased from China General Microbiological culture presevation administrative center (CGMCC) numbering
1.2696 Pediococcus acidilactici, the Pediococcus pentosaceus that it is 1.10999 purchased from CGMCC numbering that Pediococcus pentosaceus, which is, the bright string of goldbeater's skin
The Leuconostoc mesenteroides that it is 1.10327 purchased from CGMCC numbering that pearl bacterium, which is, Lactobacillus plantarum are to be purchased from CGMCC numbering
1.557 Lactobacillus plantarum, the bacillus amyloliquefaciens that it is 1.7463 purchased from CGMCC numbering that bacillus amyloliquefaciens, which are, are withered
The bacillus subtilis that it is 1.2162 purchased from CGMCC numbering that careless bacillus, which is, lactobacillus acidophilus are the numbering purchased from CGMCC
For 1.1854 lactobacillus acidophilus, pichia farinose that it is 2.707 purchased from CGMCC numbering that pichia farinose, which is, film mould
For Pichia pastoris to be 2.4061 Pichia membranaefaciens purchased from CGMCC numbering, moral gram yeast is to be received purchased from Unite States Standard biology product
The preserving number at Tibetan center (ATCC) is ATCC 10559 moral gram yeast, and Brussels moral kirschner yeast is the preservation purchased from ATCC
Number be ATCC 64276 Brussels moral kirschner yeast.
It is in the preparation process of fluid nutrient medium：
1.1 crush raw material；
Raw material after 1.2 pairs of crushing carries out deinsectization processing；
1.2.1 aluminum phosphide tablet：Meet GB5452 standards.
It is 1.2.2 stifling：Feedstock transportation enters in closed disinfection cabinet, by 3-6g/m3Dosage throw aluminum phosphide tablet is scattered
It is placed on disinfection cabinet everywhere, closed stifling 3-5 days.Disinfection cabinet is opened after stifling, divulges information and dissipates poison at least 24h.
1.3 press composition and weight configuration fluid nutrient medium in table 1.
B, strain is seeded in fluid nutrient medium and carries out liquid fermentation, obtain zymotic fluid.
Fermentation process includes：
2.1 slack tanks prepare
Prepare material-compound tank, fermentation tank, it is cleaned and sterilization is handled.
2.2 points of filter steriliseds
Sterilize total steam pressure and be maintained at 0.3 ± 0.1Mpa, filter sterilization pressure is 0.1Mpa-0.15Mpa, and the time is
30 minutes, add during sterilization raise steam slow, prevent metal filter stick from puncturing, sterilization terminates, and is passed through filtrated air drying.
2.3 charging
First add a small amount of water in material-compound tank, then by the raw material type and weight in table 1, each raw material is added to material-compound tank
In, required volume is added water, is stirred, squeezes into fermentation tank.Zymotic fluid liquid amount 60% or so.
2.4 inoculation
Strain is Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, bacillus amyloliquefaciens, withered
Careless bacillus, lactobacillus acidophilus, pichia farinose, Pichia membranaefaciens, moral gram yeast, Brussels moral kirschner yeast
Mixture.
Mixture strain adds together in fill process, and temperature is 35 DEG C when strain adds, and is stirred in inoculation.
2.5 fermented and cultured
It is 41 ± 1 DEG C to control fermentation temperature, and it is 0.3-1.3v/v.m control the ventilation ratio that ferments, speed of agitator during fermentation
150rpm--170rpm, fermentation tank inner canister pressure is zero.Installation 100W incandescent lamps, are opened during the fermentation at the top of fermentation tank, are protected
Demonstrate,prove certain illumination.Fermentation process mid-early stage throughput is big, with the continuous rising of foam, gradually reduces throughput, controls pH
Below 5.0, treat that bacterium amount reaches 5 × 10 in fermentation tank8~9After fermentation is completed, and obtains zymotic fluid.Fermentation period is controlled in 42-48
Hour.(microscopy has a small amount of saccharomycete --- investigate normative reference.)
C, the zymotic fluid in step b is taken, dry material is added into zymotic fluid, solid fermentation is carried out, obtains animal feed and add
Add agent.
The 3.1 dry material raw material compositions added into zymotic fluid and each material content are as shown in table 2, and are stirred
It is even, obtain wet feed.
3.2 solid fermentation
By wet feed by evenly mixing, loading fermentation tank, piling height is no more than 90cm, and with plastic sheeting by its table
Face is completely covered；Adjustment culture indoor environment temperature starts solid fermentation at 40-44 DEG C or so.
3.3 stirring
Solid fermentation 5-7 days (decrement bacterium) or 14-15 days (deodorization bacterium), carried out stirring every 48 hours in the process,
Control heap body core temperature to be no more than 55 DEG C, until going out finished product, ensure that material is uniform, and sample, detect sample.
D, animal feed additive is subjected to packaging storage.
In preparation process, technology controlling and process project and detection, as shown in table 3.
Embodiment 2：Animal feed additive
According to the preparation method in embodiment 1, animal feed additive is prepared.
Animal feed additive, wherein, the concentration of Pediococcus acidilactici is 8.0x 107Cfu/gm, the Pediococcus pentosaceus
Concentration is 7.0x 107Cfu/gm, the concentration of the Leuconostoc mesenteroides is 3.0x 106Cfu/gm, the Lactobacillus plantarum it is dense
Spend for 4.0x 107Cfu/gm, the concentration of the bacillus amyloliquefaciens is 5.0x 106Cfu/gm, the bacillus subtilis
Concentration be 4.0x 107Cfu/gm, the concentration of the lactobacillus acidophilus is 4.0x 106Cfu/gm, the pichia farinose
Concentration be 6.0x 107Cfu/gm, the concentration of the Pichia membranaefaciens is 9.0x 106Cfu/gm, the moral gram yeast it is dense
Spend for 8.0x 106Cfu/gm, the concentration of Brussels moral kirschner yeast is 4.0x 107cfu/gm。
Embodiment 3：The application of animal feed additive, animal feed additive are used to raise chickens
Experiment condition：The good broiler chicken of 2000 upgrowth situations is taken, is randomly divided into 4 groups, i.e. A groups, B groups, C groups and D groups,
The broiler fodder of purchase Shandong six directions Group Co., Ltd carries out separating raising.
Test method：The animal feed additive of the present invention is added into the feed of A groups, B groups, C groups and D groups, mixing is equal
Even, normal to feed each group broiler chicken, the dosage of animal feed additive is in each group feed：
A group -0, is not added, control group
B groups-feed per ton addition 250g animal feed additives
C groups-feed per ton addition 500g animal feed additives
D groups-feed per ton addition 1,000g animal feed additives
Random test：Select 250 chickens at random from each group, weighed at the 1st, 14,21,28,35 and 42 day, and collect same
The accumulative The dead quantity of one period chicken, each group broiler chicken average weight is as shown in table 4, the average day increment of each group broiler chicken is as shown in table 5,
Feed conversion rate is as shown in table 6, adds up The dead quantity as shown in table 7.
Interpretation of result：
1st, visible from the above, relative to A groups, the broiler chicken of B groups, C groups and D groups uses the dynamic of the present invention in raising
After thing feed addictive, increased weight is more notable, and The dead quantity reduces, and feed conversion rate reduces, and illustrates that the present invention is advantageous to carry
High animal growth speed, mortality of animals is reduced, efficiency of feed utilization is improved, reduces feeding cost.
2nd, in C groups, the dosage of animal feed additive is one times in B groups, and feed conversion rate is reduced in 1, D groups, is moved
The dosage of thing feed addictive is four times in B groups, and feed conversion rate but only reduces 1.7, illustrates within the specific limits, feed
Conversion ratio quickly reduces with the increase of animal feed additive, but when exceeding certain limit, with animal feed additive
Continue to increase, the reduction speed of feed conversion rate declines, to reduce overall feeding cost, the use of preferable animal feed additive
Measure and add 500g animal feed additives for feed per ton, now using effect is good, use cost is low, and overall economic efficiency is most
It is good.
Embodiment 4：The application of animal feed additive, animal feed additive are used to raise pigs
Experiment condition：
The good pig of 48 upgrowth situations is taken, is randomly divided into 2 groups, i.e. E groups and F groups, E groups are control group, and F groups are experiment
Group, the pig feed of purchase Shandong six directions Group Co., Ltd carry out separating raising.
Experimental method：
E groups：24 pigs point are in four fences, each 3 boars of fence stable breeding, 3 sows.This control group Shandong
The pig feed of six directions Group Co., Ltd is normally fed
F groups：24 pigs point are in four fences, each 3 boars of fence stable breeding, 3 sows.This experimental group is with per ton
The pig feed of Shandong six directions Group Co., Ltd added with 500g animal feed additives is normally fed.
Experiment statisticses：By the 1st day to 30 days；31st day to 60 days；1st day to 60 days, three periods were counted in each groups
The indexs such as the average daily gain (ADG) of pig, feed conversion rate (FCR), respective thickness of back fat, ill pig quantity, each period
Each indicator-specific statistics result is as shown in the table.
Interpretation of result：
Visible from the above, relative to E groups, the pig of F groups uses the animal feed additive of the present invention in raising
Afterwards, increased weight is more notable, does not occur disease condition, and feed conversion rate reduces, and respective thickness of back fat reduced, in the research of 60 days
Period, using the animal feed additive of the present invention, the average daily gain to pig improves 15.3%, and feed conversion rate reduces
9.2%, respective thickness of back fat reduces 8.9%, illustrates that the present invention is advantageous to improve animal growth speed, reduces animal dead
Rate, efficiency of feed utilization is improved, feeding cost is reduced, improves meat quality.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. animal feed additive, it is characterised in that：Mixed by following components, Pediococcus acidilactici, Pediococcus pentosaceus, goldbeater's skin
Leukonid, Lactobacillus plantarum, bacillus amyloliquefaciens, bacillus subtilis, lactobacillus acidophilus, pichia farinose, film mould
Pichia pastoris, moral gram yeast, Brussels moral kirschner yeast.
2. animal feed additive according to claim 1, it is characterised in that：The concentration of the Pediococcus acidilactici is
7.0x107cfu/gm-9.0x107Cfu/gm, the concentration of the Pediococcus pentosaceus is 6.0x107cfu/gm-8.0x107Cfu/gm,
The concentration of the Leuconostoc mesenteroides is 2.0x106cfu/gm-4.0x106Cfu/gm, the concentration of the Lactobacillus plantarum are
3.0x107cfu/gm-5.0x107Cfu/gm, the concentration of the bacillus amyloliquefaciens is 4.0x106cfu/gm-
6.0x106Cfu/gm, the concentration of the bacillus subtilis is 3.0x107cfu/gm-5.0x107Cfu/gm, the acidophilus breast bar
The concentration of bacterium is 3.0x106cfu/gm-5.0x106Cfu/gm, the concentration of the pichia farinose is 5.0x107cfu/gm-
7.0x107Cfu/gm, the concentration of the Pichia membranaefaciens is 8.5x106cfu/gm-9.5x106Cfu/gm, the moral gram yeast
Concentration be 7.0x106cfu/gm-9.0x106Cfu/gm, the concentration of Brussels moral kirschner yeast is 3.0x107cfu/
gm-5.0x107cfu/gm。
3. the preparation method of animal feed additive, it is characterised in that：Comprise the following steps, a, prepare fluid nutrient medium and bacterium
Kind, b, strain is seeded in fluid nutrient medium carries out liquid fermentation, obtain zymotic fluid, c, take zymotic fluid in step b, Xiang Fa
Dry material is added in zymotic fluid, carries out solid fermentation, obtains animal feed additive, d, animal feed additive is subjected to packaging deposited
Storage.
4. the preparation method of animal feed additive according to claim 3, it is characterised in that：In step a, Liquid Culture
Base component is included for dregs of beans, oil, starch, whole milk powder, NaCl, ZnSO4, CuSO4, MgSO4, MnSO4, FeSO4, CaSO4, work
Industry water；Strain is Pediococcus acidilactici, Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactobacillus plantarum, bacillus amyloliquefaciens, withered
Careless bacillus, lactobacillus acidophilus, pichia farinose, Pichia membranaefaciens, moral gram yeast, Brussels moral kirschner yeast
Mixture.
5. the preparation method of animal feed additive according to claim 4, it is characterised in that：In step b, liquid is prepared
During culture medium, strain adds with liquid medium starting material, and temperature is 24 DEG C -40 DEG C in seeded process, in seeded process
Stir, strain is well mixed with fluid nutrient medium.
6. the preparation method of animal feed additive according to claim 5, it is characterised in that：In step b, liquid fermentation
When, fermentation temperature is 41 ± 1 DEG C, and it is 0.3v/v.m -1.3v/v.m, speed of agitator 150rpm-- to control fermentation ventilation ratio
170rpm, fermentation tank inner canister pressure is zero, and installation incandescent lamp is with to the progress illumination of fermentation tank inside at the top of fermentation tank.
7. the preparation method of animal feed additive according to claim 6, it is characterised in that：In step b, liquid fermentation
When, fermentation process mid-early stage throughput is more than later stage throughput, and pH is less than 5.0 in fermentation tank, treats that bacterium amount reaches 5 in fermentation tank
×108~9After fermentation is completed, and the liquid fermentation cycle is 42h-48h.
8. the preparation method of animal feed additive according to claim 7, it is characterised in that：In step c, addition is done
Material is zymotic fluid, rice bran, the chaff dregs of rice, dregs of beans, salt, calcium carbonate, magnesium carbonate.
9. the preparation method of animal feed additive according to claim 8, it is characterised in that：In step c, solid fermentation
When, yeasting temperature is 40 DEG C -44 DEG C, and in solid fermentation process, stirring, control hair are carried out to fermentation reaction heap every 48h
Ferment reactor central temperature is less than 55 DEG C.
10. the application of animal feed additive, it is characterised in that：Animal feed additive is mixed in be formed in animal feed and mixed
Compound, then add 500g animal feed additives with mixture letting animals feed, dosage for animal feed per ton.
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| CN112715750A (en) * | 2020-12-31 | 2021-04-30 | 江西瑞博特生物科技有限公司 | Compound microbial agent and feed additive for livestock and poultry breeding and preparation method thereof |
| CN113261613A (en) * | 2021-06-23 | 2021-08-17 | 贵州理工学院 | Method for preserving wet white spirit vinasse and preventing mildew and feed |
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Application publication date: 20171124 |