CN107362172A - The preparation method and applications of the environmentally friendly aquatic bacterium inorganic agent of fish - Google Patents
The preparation method and applications of the environmentally friendly aquatic bacterium inorganic agent of fish Download PDFInfo
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- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
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Abstract
The aquatic bacterium inorganic agent of environmentally friendly fish that the present invention relates to the use of the preparation method of the aquatic bacterium inorganic agent of environmentally friendly fish comprising the sulphur water except malicious sulphur and prepared by the above method.
Description
Technical field
The present invention relates to the use of comprising except malicious sulphur sulphur water the aquatic bacterium inorganic agent of environmentally friendly fish preparation method and
It is applied.
Background technology
It can move freely and disease will not occur for the more fish of activity in the space of spaciousness, but in largely cultivation fish
In the plant of class, because economically the reason for cultivate in narrow space the fish of a large amount of individuals, so as to because of high-density breeding
Fish is contacted with each other and is collided, thus cause to produce scar on the skin of fish, and because of the oversupply of feed, cause to support
The water quality deterioration of field is grown, so that the health variation of the fish of plant, and the resistance of pathogenic microbes is died down, make
Pathogen easily breeds.
In addition, cause most of pathogenic microbes of fish disease all there is infectiousness, once occur once, then can be fast
Speed spread simultaneously infect other individual, therefore, in plant add eel seedling when by carry pathogen individual imported into it is foster
Grow in field, or to the eel of sequela screen when, disease's spread can be arrived during other tanks are transferred to
Overall feeding fish.
In addition, if the feed that supply is not appropriate for the fish of cultivation, or supply cause quality because of improper storage
The feed of reduction, then the health status of fish body can be deteriorated, the resistance of pathogen can be died down, so as to cause to a small amount of pathogen
Intrusion can not resist and disease occurs, also, due to it is managerial carelessness or in screening and course of conveying because skin hinder
Trace and when invading pathogenic microbes, also result in the health variation of fish body, and cause disease.
This aquatic bacterium sick (mildew) for causing fish disease seems small at parasitic initial stage because of parasitic site and unobvious
Hickie shape, but when mycelia breeds, then can become cilium shape, therefore with the naked eye can easily distinguish.
The breeding water temperature of aquatic bacterium bred at 13~20 DEG C it is vigorous, stop type water cultivate in the case of, typically 2~5
Occur between the moon and 11~December, especially concentrate and occur in 3~April of spring, the symptom shown is then as follows：Parasitic site is head
Portion, lip, trunk, afterbody etc., it is fixed to be not limited, and in having on the part of scar for fish body, then can not be limited and sent out by position
The inflammation of the raw bacterium of unboiled water.
For eel and fresh-water fishes, because of the Saprolegnia parasitica (Saprolegnia as algal fungi class (phycomycetes)
Parasitica body surface) is parasitic and causes the superinfection of the aquatic bacterium caused by single infection factor.
For Saprolegnia parasitica (Saprolegnia parasitica) bacterium, especially convey in live fish, screen, adopt ovum, adopt
Scar position caused by the operations such as essence and on the parasitic site of argulus, Gyrodactylus (Gyrodactylus), anchor worm etc., because
Caused by inflammation part, nutritive disease caused by the pathogens such as Aeromonas hydrophila (Aeromonas hydrophila)
Inflammation part, deteriorate etc. in the body surface abnormal position that is formed of single infection factor because of health, the aquatic Secondary ground of bacterium it is raw simultaneously
Parasitism, mainly invade in epithelial tissue, mycelia is expanded propagation and arrive whole body, finally make fish dead.
As described above, in order to control aquatic bacterium disease, by spraying malachite green (Malachite Green, C23H25N2Cl)
To prevent that the aquatic bacterium of fish is sick, the malachite green is also known as peacock green, and benzaldehyde is green, and victoria green B, China are green,
It is bright dark green basic-dyeable fibre, is one kind of triphenylmethane dye.
But for malachite green, in food and medicine Surveillance Authority of the U.S. (FDA in 1991；Food and Drug
Administration malachite green) is defined as carcinogen (carcinogen), and forbids being used for aquatic products, also,
Europe in 2001 has carried out the retention analysis of malachite green to Scotland cultivation salmon for the first time and has confirmed to remain, in addition,
Confirm the residual of malachite green during cultivation salmon is produced in Europe with Britain in Chile in June, 2002, and with food security
The problem of prohibit the use of malachite green.
As noted previously, as the malachite green for suppressing fish-pathogenic bacteria can not be used, therefore for fish disease can be suppressed
The needs of the aquatic bacterium inorganic agent of disease are in rising trend.
On the other hand, the present inventor is prepared for utilizing the aquatic bacterium processing of the novel environment friendly fish with the sulphur water except malicious sulphur
Agent, and be intended to its being applied to fish to suppress the aquatic bacterium for causing fish saprolegniasis.
[prior art literature]
[patent document]
(patent document 1) patent application 10-2013-0075618
(patent document 2) patent application 10-2012-0020394
(patent document 3) patent application 10-2008-0022450
The content of the invention
Technical problems to be solved
As described above in order to solve the problems, such as, the present inventor is prepared for utilizing the Novel ring with the sulphur water except malicious sulphur
The aquatic bacterium inorganic agent of fish is protected, so as to complete the present invention, wherein the aquatic bacterium inorganic agent of the fish is to make by the following method
It is standby：Make the mixed culture medium fermentation comprising green tea and privet's leaf, be then inoculated with effective microbe and cultivate, add toxicity
Cosan is last to carry out one time fermentation decomposition, crushes one time fermentation analyte with wet type colloid mill, carries out secondary fermentation point again
Solution, then it will include except malicious sulphur powder, sodium hydroxide, phosphorous acid (H3PO3) and rock salt the first mixture and include sodium metasilicate
The mixed solution of solution and Lactobacillus parafarraginis nutrient solutions is mixed and implements liquid phase, so that
Malicious sulphur is removed to prepare the initial stage that can suppress as fish saprolegniasis is caused by the use of the processing processing that toxicity is removed from sulphur
Reason it is aquatic bacterium, utilize the aquatic bacterium inorganic agent of novel environment friendly fish with the sulphur water except malicious sulphur.
Technical scheme
In order to solve the above problems, the present invention is provided using at the aquatic bacterium of environmentally friendly fish comprising the sulphur water except malicious sulphur
Manage the preparation method of agent.
In addition, the present invention provides through prepared by the above method to contain the environmentally friendly fish water comprising the sulphur water except malicious sulphur
Raw bacterium inorganic agent.
Invention effect
What it is by method preparation of the invention is ring containing the aquatic bacterium inorganic agent of the fish comprising the sulphur water except malicious sulphur
Material is protected, the advantage is that, is able to ensure that compared with the dipping method of the antimycotic medicine of commerciality using caused by therapeutic agent
The fresh food security of aquatic products, and can effectively suppress to cause the aquatic bacterium of fish saprolegniasis and improve cultured fishes
Productivity, therefore can effectively be utilized in aquaculture industry field.
Brief description of the drawings
Fig. 1 is the preparation method of the aquatic bacterium inorganic agent of environmentally friendly fish of the utilization comprising the sulphur water except malicious sulphur of the present invention
Artwork.
Fig. 2 is the photo for showing to separate water mo(u)ld and the process identified from loach surface.
Fig. 3 is the loach infected by water mo(u)ld to be handled with the aquatic bacterium inorganic agent of fish of the present invention to confirm to treat
The photo of effect.
Embodiment
In order to realize the purpose of the present invention, the present invention provides aquatic using the environmentally friendly fish comprising the sulphur water except malicious sulphur
The preparation method of bacterium inorganic agent.
The preparation method of the aquatic bacterium inorganic agent of the fish of the present invention can specifically include：
Make the step S100 to be fermented comprising green tea and privet's leaf as the mixed culture medium of carbon source；
The molasses dilution of high-temperature sterilization is added in the mixed culture medium of fermentation, effective microbe is then inoculated with and cultivates
Step S110；
Toxicity sulphur powder is added in effective microbe nutrient solution, then carries out the step S120 of one time fermentation decomposition；
The step S130 of one time fermentation analyte is crushed with wet type colloid mill；
Separation of solid and liquid is carried out to the one time fermentation analyte after broken, and effectively micro- life is added in the sediment isolated
Thing nutrient solution, then carry out the step S140 of secondary fermentation decomposition；
It is secondary except malicious sulphur fermentation analyte carries out separation of solid and liquid to what is obtained in the secondary fermentation decomposition step, and
The solid isolated is dried to prepare except malicious sulphur powder, mixing is described except malicious sulphur powder, sodium hydroxide, phosphorous
Acid (H3PO3) and rock salt the first mixture and mixing sodium silicate solution and Lactobacillus parafarraginis solution
Mixed solution mixed and implement the step S150 of liquid phase.
, can in the step S100 for making mixed culture medium ferment in the preparation method of an embodiment of the present invention
To make at 23~35 DEG C with 6.5~7.5:2.5~3.5 weight is than comprising the green tea as carbon source and privet's leaf
Mixed culture medium ferments 43~48 days, but is not limited to this.
In the preparation method of an embodiment of the present invention, in the inoculation effective microbe and the step S110 of culture
In, the molasses dilution of high-temperature sterilization can be added in the mixed culture medium of step S100 fermentation, then inoculation is effectively micro-
Biology is simultaneously cultivated 92~140 hours at 23~35 DEG C, but is not limited to this.
In the preparation method of an embodiment of the present invention, in the step S120 of the progress one time fermentation, Ke Yi
Toxicity sulphur powder is added in step S110 effective microbe nutrient solution, one time fermentation decomposition is then carried out at 25~35 DEG C
22~26 hours, but it is not limited to this.
In the preparation method of an embodiment of the present invention, the first mixture except malicious sulphur is included at described pair with mixing
Solution is mixed and implemented in the step S150 of liquid phase, and first mixture can remove malicious sulphur comprising 220~270kg
Sulphur powder, 230~260kg sodium hydroxide, 9~11kg phosphorous acid (H3PO3) and 12~17kg rock salt, and can be by institute
The first mixture is stated with mixing 18~21L sodium silicate solution and 790~830L Lactobacillus
The mixed solution of parafarraginis solution is mixed and implements liquid phase, but is not limited to this.
The present invention also provides aquatic containing the environmentally friendly fish comprising the sulphur water except malicious sulphur by above method preparation
Bacterium inorganic agent.
The aquatic bacterium inorganic agent of the fish of the present invention contains comprising the sulphur water except malicious sulphur as active ingredient, so that
The activity grown with the obstruction aquatic bacterium of such as water mo(u)ld, and following processing method can be used, i.e. raised in fish
Educate with effective dose is added in water, so that carrying out balneation with the fish subject because of the aquatic microbial disease of fish to carry out
Processing.The processing effective dose of the aquatic bacterium inorganic agent can be by those skilled in the art according to fish subject disease degree
Suitably adjusted.
Below, by embodiment, the present invention is described in detail.But following embodiments are only used for illustrating the present invention,
Present disclosure is not limited to following embodiments.
The preparation of the aquatic bacterium inorganic agent of the fish of preparation example 1.
[the step S100 for making mixed culture medium ferment]
Make to ferment as the mixed culture medium of carbon source comprising green tea and privet's leaf in the present invention.
Make at 23~35 DEG C with 6.5~7.5:2.5~3.5 weight is than including the green tea as carbon source and Japanese female
The mixed culture medium of loyal leaf ferments 43~48 days.
In addition, the mixed culture medium can be with 6.5~7.5:2.5~3.5 weight ratio includes green tea and day
The mixed culture medium that the pH for adding mineral intermixture in the carbon source mixture of this glossy privet leaf to prepare is 4.8~5.2, wherein, with
The parts by weight meter of mineral intermixture 100, be mixed with the mineral intermixture ionization of 1.5~2.5 parts by weight mineral, 8~
The fruit juice of 12 parts by weight, the natural vinegar of 2.7~3.3 parts by weight, the salt of 4.5~5.5 parts by weight, 2.7~3.3 parts by weight
The molasses of sweet ingredient and 74~84 parts by weight, but it is not limited to this.
[inoculation effective microbe and the step S110 cultivated]
The sugar of high-temperature sterilization is added in the mixed culture medium to be fermented in described the step of making mixed culture medium ferment
Sweet dilution, then it is inoculated with effective microbe and cultivates.
As set forth above, it is possible to add the molasses dilution of high-temperature sterilization in the mixed culture medium of fermentation, then it is inoculated with
Effect microorganism simultaneously cultivates 92~140 hours to prepare at 23~35 DEG C, but is not limited to this.
Preferably, effective microbe can be the mixed microorganism being made up of lactobacillus, yeast and bacterium, it is highly preferred that
Can be by Lactobacillus parafarraginis, Lactobacillus paracasei (Lactobacillus paracasei),
Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus buchneri), Lactobacillus
Harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus (Lactobacillus rhamnosus), cowpox
Lactobacillus (Lactobacillus vaccinostercus), Luo Wang acetobacters (Acetobacter lovaniensis), peroxide
Change acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris (Pichia fermentans), ethanol Candida
(Candida ethanolica), Saccharomycopsis schoenii, Thiobacillus thioxidans (Thiobacillus
Thiooxidans) and 14 kinds of effective microbes of thuringiensis (Bacillus thuringiensis) composition it is mixed
Compound, but it is not limited to this.
[the step S120 for carrying out one time fermentation decomposition]
Toxicity sulphur powder is added in the inoculation effective microbe and effective microbe nutrient solution the step of cultivate,
Then one time fermentation is carried out at 25~35 DEG C to decompose 22~26 hours.
[the step S130 that one time fermentation analyte is crushed with wet type colloid mill]
In the step of progress one time fermentation decomposition the one time fermentation analyte is crushed with wet type colloid mill.
[the step S140 for carrying out secondary fermentation decomposition]
The one time fermentation analyte crushed in described the step of crushing one time fermentation analyte with wet type colloid mill is carried out
Separation of solid and liquid (solid-liquid separation), and the inoculation effective microbe is added in the sediment isolated
And the effective microbe nutrient solution in the step of cultivating, then carry out secondary fermentation decomposition.
[to the step S150 mixed comprising the first mixture except malicious sulphur with mixed solution]
It is secondary except malicious sulphur fermentation analyte carries out separation of solid and liquid to what is obtained in the secondary fermentation decomposition step, and
The solid isolated is dried to prepare except malicious sulphur powder, mixing is described except malicious sulphur powder, sodium hydroxide, phosphorous
Acid (H3PO3) and rock salt the first mixture and mixing sodium silicate solution and Lactobacillus parafarraginis solution
Mixed solution mixed and implement liquid phase.
First mixture as described above removes malicious sulphur powder, 230~260kg hydroxide comprising 220~270kg
Phosphorous acid (the H of sodium, 9~11kg3PO3) and 12~17kg rock salt.
The mixed solution mixed in the first mixture as described above include 18~21L sodium silicate solution and 790~
830L Lactobacillus parafarraginis solution.
It is highly preferred that first mixture can mix 250kg except malicious sulphur powder, 250kg sodium hydroxide,
10kg phosphorous acid (H3PO3) and 15kg rock salt, and stirred 66~78 hours at 22~28 DEG C and implement liquid phase to make
It is standby.
Phosphorous acid (H in first mixture3PO3) it is different from phosphoric acid (H3PO4), it has uncommon P-H in nature
Structure, therefore can hinder and to form the oomycetes of the zoospores such as epidemic disease bacterium, pythium (Pythium) and downy mildew
(Oomycetes) phosphate metabolism effect, so as to directly kill pathogen or suppress growth and breeding, and to domestic animal fish and
The toxicity of animalcule is very low, hardly with worry environmental pollution.
In addition, for the mixed solution mixed in the first mixture, it is highly preferred that being mixed in first mixture
Conjunction is mixed to 20L sodium silicate solution and 810L Lactobacillus parafarraginis solution and implements liquid phase
The mixed solution of change.
The Lactobacillus parafarraginis bacterial strains are to make use of the state-run agricultural sciences for being isolated from plant
Institute's agricultural genetic resources center preserving number：KACC91710P bacterial strains.
As described above, the mixed solution is mixed in the first mixture, so as to prepare the sulphur water included except malicious sulphur.
The sulphur water removes malicious sulphur to remove toxicity from sulphur, and it may be used as suppressing at the aquatic bacterium of aquatic bacterium
Agent is managed, wherein the aquatic bacterium is the reason at initial stage for causing fish saprolegniasis.
The Lactobacillus parafarraginis solution is effective microbe, can be by lactobacillus, yeast
And the mixed microorganism of bacterium composition, it is highly preferred that can be by Lactobacillus parafarraginis, secondary cheese breast
Bacillus (Lactobacillus paracasei), Lactobacillus tolerans, Bu Shi lactobacillus (Lactobacillus
Buchneri), Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus
(Lactobacillus rhamnosus), Lactobacillus vaccinostercus (Lactobacillus vaccinostercus), Luo Wang acetobacters
(Acetobacter lovaniensis), peroxidating acetobacter (Acetobacter peroxydans), fermentation Pichia pastoris
(Pichia fermentans), ethanol Candida (Candida ethanolica), Saccharomycopsis
Schoenii, Thiobacillus thioxidans (Thiobacillus thiooxidans) and thuringiensis (Bacillus
Thuringiensis) the mixture of 14 kinds of effective microbes of composition, but it is not limited to this.
The utilization prepared by method as described above is malicious with removing except the aquatic bacterium inorganic agent of environmentally friendly fish of malicious sulphur
Property implement liquid phase except malicious sulphur, be in order to suppress to turn into cause fish saprolegniasis initial stage reason aquatic bacterium.
Active analysis of the embodiment 1. for suppression water mo(u)ld
The separation identification of 1-1. water mo(u)lds
For the utilization to the present invention except the activity of the suppression water mo(u)ld of the aquatic bacterium inorganic agent of environmentally friendly fish of malicious sulphur is entered
Row evaluation, separate water mo(u)ld from the epidermis of loach and identified.
As shown in Fig. 2 experimentation is：Water mo(u)ld is separated from the epidermis of the loach with saprolegniasis and carries out pure culture,
Then 18S rDNA analyses are implemented, it is thus identified that separate water mo(u)ld (Saprolegnia parasitica (the parasitic water of identification
It is mould)).
Active evaluations of the 1-2. for the suppression water mo(u)ld for removing malicious sulphur water using the aquatic bacterium inorganic agent of fish
In the fluid nutrient medium for removing malicious sulphur water, hydrogen peroxide and formalin processing of the invention with various concentrations
The result that is confirmed of effect to suppressing water mo(u)ld, it is thus identified that the aquatic bacterium inorganic agent of fish of the invention except malicious sulphur water and
Hydrogen peroxide shows the activity for suppressing water mo(u)ld more than 30ppm concentration, and formalin shows suppression in more than 10ppm
The activity of water mo(u)ld.It was furthermore observed that compared with the present invention is except malicious sulphur water, hydrogen peroxide and formalin are 50ppm's
Suppress active higher (table 1) of water mo(u)ld under concentration.
[table 1]
Active evaluation for suppressing water mo(u)ld (Saprolegnia parasitica (Saprolegnia parasitica))
(-, unrestraint activity；+, the degree of inhibitory activity)
Analysis of the embodiment 2. for the minimal inhibitory concentration of fish-pathogenic bacteria
Various fish pathogens bacterium is handled with the malicious sulphur water that removes of the aquatic bacterium inorganic agent of environmentally friendly fish of the present invention, and
Analyze minimal inhibitory concentration (MIC, minimum inhibitory concentration).
Experiment condition is that vibrio (Vibrio sp.), Y.rucker are inoculated with solid medium
(Yersinia ruckeri) and Aeromonas hydrophila (Aeromonas hydrophila) (being above Gram-negative bacteria) and
Accessory mamma streptococcus (Streptococcus parauberis) (gram-positive bacteria) simultaneously carries out preculture, then with difference
The of the invention of concentration is trained using the liquid handled except malicious sulphur water of the aquatic bacterium inorganic agent of environmentally friendly fish except malicious sulphur
Support in base and be inoculated with the bacterium, confirm the concentration of bacteria growing inhibiting development after 24 hours,.
As a result it has been confirmed that in the case of Gram-negative bacteria, with all concentration ranges for removing malicious sulphur water process
All do not show the activity of bacteria growing inhibiting, only in the case of gram-positive bacteria accessory mamma is streptococcic, 25ppm with
On processing section inhibit the growth (table 2) of bacterium.
[table 2]
For fish-pathogenic bacteria except the evaluation of the minimal inhibitory concentration (MIC) of malicious sulphur water
(+realize is grown ,-suppress to grow)
Embodiment 3. utilizes the oxicity analysis of water flea
Using water flea to the aquatic bacterium inorganic agent of environmentally friendly fish of the invention except the toxicity of malicious sulphur water is confirmed.It is real
Test is according to South Korea's No. 2015-8 (2015-04-30) " test method on chemical substance of state-run environmental science institute bulletin
The Section 2 water flea acute toxicity test (OECD TG 202) in the chapter 3 ecology influence experiment field of regulation ", in South Korea's chemistry
Test for fusion institute implements.
Briefly, experiment is implemented in the following manner：Using the water fleas of 3 week old, (Daphnia magna are (large-scale
Flea)) female individual production, it is raw after less than the immature individual of 24 hours, and OECD TG 202M4 culture mediums are used as feeding and used
Water, average temperature are set to 20.1 DEG C (20.0~20.3 DEG C), and average pH is set to 8.25 (7.68~9.45), average dissolution oxygen (DO)
7.91mg/l (7.63~8.17mg/l) is set to, and with the light (08 of 16 hours in 1L glass beaker:00~24:And 8 00)
Dark (the 24 of hour:00~8:00) optical condition of condition maintains the testing liquid stop type water of 48 hours to implement.Except malicious sulphur water
Concentration for the treatment of be with 100mg/l nominal concentration (nominal concentration) implement the limit test.As toxicity
The positive controls of experiment, then potassium bichromate (Potassium dichromate) is used.
Non mobility and the result analyzed of taking action extremely to water flea, it is known that without generation water during experiment
The swimming of flea hinders, and general poisoning symptom and specific symptoms (table 3 and table 4) are not observed completely.
[table 3]
Analysis for the accumulative non mobility of water flea
[table 4]
The abnormal action of water flea
In addition, using water flea as object to the EC except malicious sulphur water50(medium effective concentration, half maximal
Effective concentration) result that is confirmed, it is thus identified that under the conditions of 48 hours, in the dense of more than 100ppm
50% or so in the lower water flea of degree shows that swimming hinders (table 5).
[table 5]
Except the EC of malicious sulphur water50The analysis of value
The analysis of the effect of fish of loach of the embodiment 4. for being infected by saprolegniasis aquatic bacterium inorganic agent
It is aquatic in the fish of the invention of 100ppm concentration in order to confirm the effect of the aquatic bacterium inorganic agent of fish of the present invention
After the removing of bacterium inorganic agent carries out dipping 24 hours in malicious sulphur water to the loach that is infected by saprolegniasis, confirmed mud after 5 days
The state of loach.
As a result it has been confirmed that implementing 24 with the loach for removing malicious sulphur water process of the aquatic bacterium inorganic agent of fish of the present invention
The infection of water mo(u)ld is treated (Fig. 3) behind 5 days after the dipping of hour.
Claims (6)
1. utilize the preparation method of the aquatic bacterium inorganic agent of the environmentally friendly fish comprising the sulphur water except malicious sulphur, it is characterised in that bag
Include：
Make the step S100 to be fermented comprising green tea and privet's leaf as the mixed culture medium of carbon source；
The molasses dilution of high-temperature sterilization is added in the mixed culture medium of fermentation, is then inoculated with effective microbe and the step cultivated
Rapid S110；
Toxicity sulphur powder is added in effective microbe nutrient solution, then carries out the step S120 of one time fermentation decomposition；
The step S130 of one time fermentation analyte is crushed with wet type colloid mill；
Separation of solid and liquid is carried out to the one time fermentation analyte after broken, and effective microbe training is added in the sediment isolated
Nutrient solution, then carry out the step S140 of secondary fermentation decomposition；
It is secondary except malicious sulphur fermentation analyte carries out separation of solid and liquid to what is obtained in the secondary fermentation decomposition step, and to dividing
The solid separated out is dried to prepare except malicious sulphur powder, will mixing it is described except malicious sulphur powder, sodium hydroxide, phosphorous acid and
First mixture of rock salt and mixing sodium silicate solution and the mixed solution of Lactobacillus parafarraginis solution
Mixed and implement the step S150 of liquid phase.
2. the preparation according to claim 1 using the aquatic bacterium inorganic agent of the environmentally friendly fish comprising the sulphur water except malicious sulphur
Method, it is characterised in that include at described Dui except the first mixture of malicious sulphur is mixed with mixed solution and implements liquid phase
In the step S150 of change, first mixture removes malicious sulphur powder, 230~260kg hydroxide comprising 220~270kg
The rock salt of sodium, 9~11kg phosphorous acid and 12~17kg, and first mixture and the sodium metasilicate for mixing 18~21L is molten
The mixed solution of liquid and 790~830L Lactobacillus parafarraginis solution is mixed and implements liquid phase
Change.
3. the preparation according to claim 1 using the aquatic bacterium inorganic agent of the environmentally friendly fish comprising the sulphur water except malicious sulphur
Method, it is characterised in that in the step S100 for making mixed culture medium ferment, make at 23~35 DEG C with 6.5~7.5:
2.5~3.5 weight comprising the mixed culture medium of the green tea as carbon source and privet's leaf than fermenting 43~48 days.
4. the preparation according to claim 1 using the aquatic bacterium inorganic agent of the environmentally friendly fish comprising the sulphur water except malicious sulphur
Method, it is characterised in that in the inoculation effective microbe and the step S110 of culture, in the mixing of step S100 fermentation
The molasses dilution of high-temperature sterilization is added in culture medium, is then inoculated with effective microbe and the culture 92~140 at 23~35 DEG C
Hour.
5. the preparation according to claim 1 using the aquatic bacterium inorganic agent of the environmentally friendly fish comprising the sulphur water except malicious sulphur
Method, it is characterised in that in the step S120 of the progress one time fermentation, in step S110 effective microbe nutrient solution
Toxicity sulphur powder is added, one time fermentation is then carried out at 25~35 DEG C decomposes 22~26 hours.
6. contain the environmentally friendly fish for including the sulphur water except malicious sulphur by prepared by the method for any one of Claims 1 to 5
Aquatic bacterium inorganic agent.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020160057883A KR101654253B1 (en) | 2016-05-12 | 2016-05-12 | Manufacturing method and the usage of eco-friendly aquatic fungicidal agent composed of the water containing detoxified sulfur for use in fish. |
| KR10-2016-0057883 | 2016-05-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107362172A true CN107362172A (en) | 2017-11-21 |
Family
ID=56950371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710221638.1A Pending CN107362172A (en) | 2016-05-12 | 2017-04-06 | The preparation method and applications of the environmentally friendly aquatic bacterium inorganic agent of fish |
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| KR101738547B1 (en) | 2016-11-16 | 2017-05-22 | 이승협 | Composition for spray or spread of fruit bag for fruit tree comprising mixture of detoxificated sulfur powder as effective component and uses thereof |
| KR101926732B1 (en) * | 2018-04-11 | 2019-03-07 | 김일호 | A manufacturing method for eco-friendly composition using by EM(Effective Micro Organism) of metabolites and the eco-friendly composition made using it |
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| KR20080022450A (en) | 2006-09-06 | 2008-03-11 | 삼성전자주식회사 | Phase change material memory device and mehtod for forming the same |
| KR101780440B1 (en) | 2010-08-30 | 2017-09-22 | 삼성전자 주식회사 | Output Controling Method Of List Data based on a Multi Touch And Portable Device supported the same |
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| KR101334157B1 (en) * | 2013-03-12 | 2013-11-29 | 농업회사법인 주식회사 엘바이오텍 | Surfur removing poison produced using effective microorganism and uses thereof |
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