CN107356582A - Laser metabolic evaluation instrument and its detection method - Google Patents
Laser metabolic evaluation instrument and its detection method Download PDFInfo
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- CN107356582A CN107356582A CN201710682083.0A CN201710682083A CN107356582A CN 107356582 A CN107356582 A CN 107356582A CN 201710682083 A CN201710682083 A CN 201710682083A CN 107356582 A CN107356582 A CN 107356582A
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- 238000011156 evaluation Methods 0.000 title claims abstract description 47
- 230000002503 metabolic effect Effects 0.000 title claims abstract description 47
- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 46
- 210000002700 urine Anatomy 0.000 claims abstract description 45
- 210000004369 blood Anatomy 0.000 claims abstract description 31
- 239000008280 blood Substances 0.000 claims abstract description 31
- 208000015924 Lithiasis Diseases 0.000 claims abstract description 27
- 239000000203 mixture Substances 0.000 claims abstract description 25
- 230000003287 optical effect Effects 0.000 claims abstract description 13
- 238000005070 sampling Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 15
- 238000001228 spectrum Methods 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 5
- 239000012744 reinforcing agent Substances 0.000 claims description 4
- 238000005259 measurement Methods 0.000 abstract description 25
- 239000004575 stone Substances 0.000 abstract description 12
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 abstract description 8
- 229960003067 cystine Drugs 0.000 abstract description 8
- 230000002265 prevention Effects 0.000 abstract description 5
- 230000002485 urinary effect Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 31
- 238000003745 diagnosis Methods 0.000 description 13
- 201000010099 disease Diseases 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 150000002500 ions Chemical class 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 235000006408 oxalic acid Nutrition 0.000 description 10
- 239000003814 drug Substances 0.000 description 7
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 5
- 150000001768 cations Chemical class 0.000 description 4
- VAAUVRVFOQPIGI-SPQHTLEESA-N ceftriaxone Chemical compound S([C@@H]1[C@@H](C(N1C=1C(O)=O)=O)NC(=O)\C(=N/OC)C=2N=C(N)SC=2)CC=1CSC1=NC(=O)C(=O)NN1C VAAUVRVFOQPIGI-SPQHTLEESA-N 0.000 description 4
- 229960004755 ceftriaxone Drugs 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 229940075420 xanthine Drugs 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000003466 anti-cipated effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- QXDMQSPYEZFLGF-UHFFFAOYSA-L calcium oxalate Chemical compound [Ca+2].[O-]C(=O)C([O-])=O QXDMQSPYEZFLGF-UHFFFAOYSA-L 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 230000001524 infective effect Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- -1 Amine glycan Chemical class 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 102100030386 Granzyme A Human genes 0.000 description 1
- 101001009599 Homo sapiens Granzyme A Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000001237 Raman spectrum Methods 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000009535 clinical urine test Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 208000014001 urinary system disease Diseases 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
Abstract
Laser metabolic evaluation instrument, including Raman spectrometer, Raman spectrometer are detected in blood of human body and urine to lithiasis because of related composition, and Raman spectrometer includes laser head, Raman optical filtering, microscope and detection part, in addition to automatic sampling apparatus.A kind of detection method of laser metabolic evaluation instrument, comprises the following steps：(1) calculus Related Component is determined；(2) the Raman spectrogram of calculus Related Component is obtained；(3) tested blood of human body and urine specimen are detected by Raman spectrometer, obtained by the calculus Related Component content in blood of human body and urine specimen.Beneficial effects of the present invention are：1st, the measurement range of stone in urinary system automation metabolic evaluation has been expanded；2nd, detection efficiency and precision are improved；3rd, the measurement to the index such as macromolecular calculus correlation factor, cystine, P elements in urine is by being very unlikely to become possibility, and these indexs for make clear lithiasis because and to improve prevention scheme particularly important.
Description
Technical field
The present invention relates to a kind of medicine equipment, more particularly, to a kind of instrument and its inspection that metabolic evaluation is carried out using laser
Survey method.
Background technology
Stone in urinary system is a kind of complicated, multi-pathogenesis disease, is the coefficient result of E＆H factor.It is high
Recurrence rate is the maximum feature of the disease.Most calcium oxalate calculus formers can find the cause of disease by metabolic evaluation, its result
To the further treatment and prevention important in inhibiting of patient.Domestic and international expert has reached common understanding, to stone in urinary system patient
Metabolic evaluation should be carried out.But it is cumbersome because traditional metabolic evaluation technical efficiency is low, so in practice, both at home and abroad
In addition to a few studies mechanism, metabolic evaluation seldom clinically is carried out to lithiasis patient.
In the research of early stage, we devise a metabolic evaluation device based on ion chromatograph, can automate
Measure in urine it is main into the concentration of the stone factor and calculus inhibiting factor (oxalic acid, uric acid, citric acid, calcium ion and magnesium from
Son), and give preliminary diagnosis.But stone in urinary system disease is a kind of multi-pathogenesis disease after all, to its complete etiological diagnosis
Need to measure more urines even physiochemical indice, the measurement results of above-mentioned five kinds of ions can not cover all lithiasises because,
Preliminary diagnosis can only be carried out to the cause of disease of most common calcinm oxalate calculus (to run into the case of complexity, still need to combine other indexs
Measurement).And automaticity highest ion chromatograph or Biochemical Analyzer can not be completed to own in urine, blood at present
The related organic molecule of the calculus even measure of macromolecular.Calculus Related Component (such as T-M osteopontins, Portugal in the urine of part
Amine glycan etc.) measuring method only rest on laboratory applications, because its measurement efficiency is low, and can not be applied to clinic.And urinate
The measure of P elements in liquid, there is also difficulty at present.Measured with the chromatography of ions, because molecular conformation of the P elements in water by
Change occurs in the influence of pH value, and ion chromatograph, during measurement, from sample introduction to elution process, liquid pH value is always
Can change, thus can not accurate measurement phosphorus from urine element concentration.And the Biochemical Analyzer of colorimetric method measurement is used, though
Automation so is realized, but measurement accuracy is limited, and clinical effectiveness is only for reference.Cystine in urine, xanthine material inspection
Survey, also only reside within laboratory stage at present, can not large area be applied to clinic.Moreover, receiving the limitation of technology, pass
The lithiasis metabolic evaluation method of system can be only used to be metabolized reason caused by calculus, for heredity calculus such as cystine stone with
And Drug calculus, as ceftriaxone calculus needs to do special inspection, even combines patient medical history and infers and can just find the cause of disease.
Can not be that clinic efficiently, easily provides accurately and comprehensive laboratory foundation.
The assay method of existing oxalic acid, citric acid --- the chromatography of ions there is also it is intrinsic the defects of.It is, in principle, that
The chromatography of ions is the technology before a kind of 20 years, detects electric signal caused by liquid intermediate ion clearing house by detector, realizes
The measure of substances in liquids concentration.Due to the defects of electrical signal detection is intrinsic in liquid, although chromatography of ions precision in theory
Ppm ranks can be reached, but also only can be used for the measurement of organic molecule such as oxalic acid in urine reluctantly, and practical operation
The defects of jitter, measuring result error is big in journey be present, sometimes measurement error can reach 30% in real process
More than.Especially for the measure of cation, because cation peak value is small, area is also relatively small under peak value, many times positive
Quasi-molecular ions is in Fusion Strain, it is necessary to be separated by graphics process with other peaks, undoubtedly increases cation measurement
Error.And colorimetric method is used, and first, because error is larger, it can not effectively measure organic molecule；Second, cation measurement knot
Although fruit can meet clinical demand, the larger risk for even resulting in mistaken diagnosis of error is still suffered from.
The content of the invention
The invention aims to solve the defects of present in metabolic evaluation in the prior art, there is provided one kind utilizes laser
The instrument for carrying out metabolic evaluation solves the above problems.
To achieve these goals, technical scheme is as follows：
A kind of laser metabolic evaluation instrument, including Raman spectrometer, it is characterised in that the Raman spectrometer detects human body blood
To lithiasis because of related composition in liquid and urine, and the Raman spectrometer includes the optical wavelength and light of adjustable incident light
Laser head, Raman optical filtering, microscope and the detection part of intensity, in addition to for auto injection and add surface reinforcing agent
Automatic sampling apparatus.
Above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that the detection part is one of following：Photoelectricity is examined
Survey device, wide spectrum spectrometer and CCD-detector.
Above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that also counted including induced with laser spectrometer and center control
Calculation machine, the induced with laser spectrometer are detected in calculus to lithiasis because of related composition.
Above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that also including fluorescence detector, the fluorescence detector inspection
Survey in calculus to lithiasis because of related composition.
Above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that the Raman spectrometer detects human whole blood sample.
A kind of above-mentioned laser metabolic evaluation instrument, it is characterised in that the Raman spectrometer detection human body at least 24 hours
To lithiasis because of related composition in urine above.
Above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that in the Raman spectrometer detection human body single urine
To lithiasis because of related composition.
A kind of detection method of laser metabolic evaluation instrument, it is characterised in that comprise the following steps：
(1) all calculus Related Components being currently known are determined；
(2) by the optical wavelength and luminous intensity of the laser head for adjusting Raman spectrometer, all calculus being currently known
The Raman spectrogram of Related Component；
(3) tested blood of human body and urine specimen are detected by Raman spectrometer, obtained by blood of human body and urine
Calculus Related Component content in liquid sample.
The detection method of above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that the Raman spectrometer detects human body
Whole blood sample.
A kind of above-mentioned laser metabolic evaluation instrument, it is characterised in that the Raman spectrometer detection human body at least 24 hours
To lithiasis because of related composition in urine above.
Above-mentioned a kind of laser metabolic evaluation instrument, it is characterised in that in the Raman spectrometer detection human body single urine
To lithiasis because of related composition.
Beneficial effects of the present invention are：1st, the measurement range of stone in urinary system automation metabolic evaluation has been expanded；2nd, improve
Detection efficiency and precision；3rd, the measurement to the index such as macromolecular calculus correlation factor, cystine, P elements in urine is by can not
Can become possible to, and these indexs for make clear lithiasis because and to improve prevention scheme particularly important.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation of Raman spectrometer.
Embodiment
The effect of to make to architectural feature of the invention and being reached, has a better understanding and awareness, to preferable
Embodiment and accompanying drawing coordinate detailed description, are described as follows：
Referring to Fig. 1 and Fig. 2, a kind of laser metabolic evaluation instrument, including Raman spectrometer 100, the and of induced with laser spectrometer 200
Central control computer 300, Raman spectrometer 100 are detected in blood of human body and urine to lithiasis because of related composition, laser
Induce spectrometer 200 to detect in calculus to lithiasis because of related composition, in the present invention, fluorescence detector can also be included,
Induced with laser spectrometer 200 can also be substituted using fluorescence detector.Advantage of the Raman spectrum when detecting body fluid sample
It is that photon is not likely to produce splash effect after entering liquid, caused spectrogram background noise is low, specificity is good.Laser-induced optical
Splash effect is also easy to produce after spectrum photon incidence liquid, so being used for the detection of solid sample.
Raman spectrometer 100 includes the optical wavelength of adjustable incident light and laser head 110, the Raman optical filtering of luminous intensity
120th, microscope 130 and detection part 140, detection part are photoelectric detector, wide spectrum spectrometer or CCD-detector, in addition to
For auto injection and the automatic sampling apparatus 400 of addition surface reinforcing agent.And for laser head 110, its optical wavelength adjustment model
Covering 300-1000nm is enclosed, its luminous intensity is realized by adjusting laser power, Power coverage 10W-300W, by adjusting luminous intensity
And optical wavelength so that for the measurement of different indexs, background noise can be preferably minimized, and the spectrogram of tested substance is the most
Substantially, measurement accuracy highest.
In the present invention, Raman spectrometer 100 detect human whole blood sample, and it is conventional in the prior art, detecting
, it is necessary to be centrifuged to blood during each element component content in blood of human body, in centrifugal process, red blood cell rupture is substantial amounts of careless
Acid is released into blood plasma, so as to cause blood concentration of oxalic acid can not Accurate Determining.
And simultaneously, Raman spectrometer 100 detect the human body urine of at least more than 24 hours in lithiasis because it is related into
Point or single urine in lithiasis because of related composition.When carrying out calculus etiological analysis metabolic evaluation, receipts are generally required
Collection patient's twenty-four-hour urine liquid carries out composition detection again after being acidified or being alkalized, and for Infective calculus, single measurement urine
The content of middle urea decomposing enzyme can be made a definite diagnosis, avoid stone composition analysis need calculus sample, urine Bacteria Culture interference compared with
Greatly, easy to cause missed diagnosis and mistaken diagnosis.For Drug calculus, such as ceftriaxone calculus, single urine drug concentration is anticipated with more diagnosis
Justice.
For the ease of sample introduction, Raman spectrometer 100 of the invention also includes being used for auto injection and addition surface reinforcing agent
Automatic sampling apparatus 400, automatic sampling apparatus 400 can auto injection, improve the present invention automaticity.
A kind of detection method of laser metabolic evaluation instrument, comprises the following steps：
(1) all calculus Related Components being currently known are determined；
(2) by the optical wavelength and luminous intensity of the laser head for adjusting Raman spectrometer, all calculus being currently known
The Raman spectrogram of Related Component；
(3) tested blood of human body and urine specimen are detected by Raman spectrometer, obtained by blood of human body and urine
Calculus Related Component content in liquid sample.
In the present invention, Raman spectrometer 100 detect human whole blood sample, and it is conventional in the prior art, detecting
, it is necessary to be centrifuged to blood during each element component content in blood of human body, in centrifugal process, red blood cell rupture is substantial amounts of careless
Acid is released into blood plasma, so as to cause blood concentration of oxalic acid can not Accurate Determining.
And simultaneously, Raman spectrometer 100 detect the human body urine of at least more than 24 hours in lithiasis because it is related into
Point or single urine in lithiasis because of related composition.When carrying out calculus etiological analysis metabolic evaluation, receipts are generally required
Collection patient's twenty-four-hour urine liquid carries out composition detection again after being acidified or being alkalized, and for Infective calculus, single measurement urine
The content of middle urea decomposing enzyme can be made a definite diagnosis, avoid stone composition analysis need calculus sample, urine Bacteria Culture interference compared with
Greatly, easy to cause missed diagnosis and mistaken diagnosis.For Drug calculus, such as ceftriaxone calculus, single urine drug concentration is anticipated with more diagnosis
Justice.
For the automation metabolic evaluation instrument for comparing previous generation, new metabolic evaluation instrument is designed based on spectrum, there is following wound
New point：
Older generation's metabolic evaluation instrument realizes sample using ion chromatograph and liquid chromatograph as hardware foundation by Curve guide impeller
The automation that product examine is surveyed, but the detection method based on liquid chromatogram/chromatography of ions is applied in urine, and its testing accuracy is only capable of
Reach ppm ranks, reach the lower limit of measurement accuracy reluctantly, therefore, the less stable measured in practical application, generally require more
Secondary measurement check and correction.
Older generation's metabolic evaluation instrument using calcium oxalate calculus formers as predominantly detect/assess object, for rare heredity
Calculus --- such as cystine stone/xanthic calculus and Drug calculus --- is as ceftriaxone calculus is helpless.
For this part of calculus patient, as calculus is larger, still composition is carried out by surgical operation, taking out calculus
The mode of analysis determines its cause of disease.But when calculus is smaller, clinician has no idea to screen using older generation's metabolic evaluation instrument
Go out its cause of disease, sample can not be also obtained by way of surgical operation.Therefore clinician's can be adopted method is limited, or
Selection observation waits, and sits by and watch calculus and grows up；Or the cause of disease of the method "ball-park" estimate calculus by inquiring medical history and family history,
This can bring the deviation in prevention scheme；Or by the detection methods in other laboratories come the examination cause of disease --- such as detection urine
The excretion of middle cystine or xanthine.But has a problem in that a not every hospital can have the configuration of this detectability,
Because the excretion detection of the material such as cystine or xanthine rests on the realities of scientific research institutions or teaching hospital more at present in urine
Test room, can not large-area applications in clinic --- particularly basic hospital clinical detection.So, patient will be as headless fly
The same migration is between each laboratory, or even the detection method of part index number only stays in laboratory level (such as urine
In GAGs, TF albumen etc., the methods of being only capable of passing through protein spectrum detects, and testing cost is high), clinic can not be applied to, it is right
In the patient of these rare calculus, can find the cause of disease can only Time-Dependent and fortune, patient often takes a lot of time, etc.
The cause of disease is have found, calculus is also grown up.The species coverage rate of raman spectroscopy measurement material is big, can be to rare knot applied to clinic
Stone patient is screened, and the early stage of rare calculus patient is precisely prevented so as to realize.
Blind area --- the measure of blood medium-height grass acid concentration of a diagnosis and measurement be present in traditional metabolic evaluation method.By
So-called blood concentration of oxalic acid measure is in fact inaccurate in text, because traditional measurement method needs to carry out the centrifugation of blood, from
During the heart, red blood cell rupture, substantial amounts of oxalic acid is released into blood plasma, so as to cause blood concentration of oxalic acid can not Accurate Determining.But draw
Graceful spectrum can realize seamless/trace detection, in laser irradiation process even can not damaged cells red blood, so avoid red
Cell rupture discharges oxalic acid, it is achieved thereby that the precise determination of blood concentration of oxalic acid.
Beneficial effects of the present invention are：1st, the measurement range of stone in urinary system automation metabolic evaluation has been expanded；2nd, improve
Detection efficiency and precision；3rd, the measurement to the index such as macromolecular calculus correlation factor, cystine, P elements in urine is by can not
Can become possible to, and these indexs for make clear lithiasis because and to improve prevention scheme particularly important.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
For personnel it should be appreciated that the present invention is not limited to the above embodiments, that described in above-described embodiment and specification is the present invention
Principle, various changes and modifications of the present invention are possible without departing from the spirit and scope of the present invention, these change and
Improvement is both fallen within the range of claimed invention.The protection domain of application claims by appended claims and its
Equivalent defines.
Claims (10)
1. laser metabolic evaluation instrument, including Raman spectrometer, it is characterised in that the Raman spectrometer detection blood of human body and urine
To lithiasis because of related composition in liquid, and the Raman spectrometer includes the optical wavelength and luminous intensity of adjustable incident light
Laser head, Raman optical filtering, microscope and detection part, in addition to for auto injection and add entering automatically for surface reinforcing agent
Sampling device.
2. laser metabolic evaluation instrument according to claim 1, it is characterised in that also including induced with laser spectrometer and center
Control computer, the induced with laser spectrometer are detected in calculus to lithiasis because of related composition.
3. laser metabolic evaluation instrument according to claim 1, it is characterised in that also including fluorescence detector, the fluorescence
To lithiasis because of related composition in detector detection calculus.
4. the laser metabolic evaluation instrument according to Claims 2 or 3, it is characterised in that the Raman spectrometer detects human body
Whole blood sample, the detection part are one of following：Photoelectric detector, wide spectrum spectrometer and CCD-detector.
5. laser metabolic evaluation instrument according to claim 4, it is characterised in that the Raman spectrometer detection human body is at least
To lithiasis because of related composition in the urine of more than 24 hours.
6. laser metabolic evaluation instrument according to claim 4, it is characterised in that the Raman spectrometer detects human body single
To lithiasis because of related composition in urine.
7. the detection method of laser metabolic evaluation instrument, it is characterised in that comprise the following steps：
(1) all calculus Related Components being currently known are determined；
(2) by the optical wavelength and luminous intensity of the laser head for adjusting Raman spectrometer, all calculus being currently known are related
The Raman spectrogram of composition；
(3) tested blood of human body and urine specimen are detected by Raman spectrometer, obtained by blood of human body and urine mark
Calculus Related Component content in this.
8. the detection method of laser metabolic evaluation instrument according to claim 7, it is characterised in that the Raman spectrometer inspection
Survey human whole blood sample.
9. laser metabolic evaluation instrument according to claim 8, it is characterised in that the Raman spectrometer detection human body is at least
To lithiasis because of related composition in the urine of more than 24 hours.
10. laser metabolic evaluation instrument according to claim 9, it is characterised in that the Raman spectrometer detects human body list
To lithiasis because of related composition in secondary urine.
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| US20090066934A1 (en) * | 2005-07-14 | 2009-03-12 | Johnway Gao | Optical devices for biological and chemical detection |
| CN104568900A (en) * | 2014-12-26 | 2015-04-29 | 中国农业大学 | Automatic dripping and coating device for surface enhanced Raman solution for detecting agricultural products |
| CN105954257A (en) * | 2016-05-24 | 2016-09-21 | 厦门市卓厦光电科技有限公司 | Intelligent human body calculus component analyzer and application method thereof |
-
2017
- 2017-08-10 CN CN201710682083.0A patent/CN107356582A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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