CN107354470A - A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria and cleaning - Google Patents

Abstract

The invention discloses a kind of magnetic silica to fix the anti-oxidant compound powder cleaning agent of degradation bacteria, is made up of following raw materials according：Sodium hydroxide, sodium carbonate, metasilicate pentahydrate sodium, silicon silicic acid organic carboxyl acid polymeric modification thing, neopelex, polyepoxy sodium succinate, coconut oil diethanol amine, isomery undecyl alcohol APEO, hexamethylene, poly- MTES, silane coupler kh550, nanometer magnetofluid, picoline, Sodium hydroxide q. s, 0.9% sodium chloride are appropriate, appropriate absolute ethyl alcohol, appropriate deionized water；Powder cleaning agent prepared by the present invention is not phosphorous, environmentally friendly, and cleaning performance is better than conventional pre-treatment oil removing process, and service life is more lasting, nontoxic to environment, uses manpower and material resources sparingly, and use range is wide, and caused waste water is more disposable.

Description

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria and clear Wash technique

Technical field

The present invention relates to metal conditioner technical field, more particularly to a kind of magnetic silica to fix degradation bacteria and resist Aoxidize compound powder cleaning agent and cleaning.

Background technology

Metal is carrying out before processing, it is necessary to washes the dirts such as antirust oil, the cutting fluid on surface, traditional metal surface is clear Washing lotion is formulated based on alkali, phosphate builder, nonyl phenol or octyl group phenols surfactant, operating temperature during cleaning Generally higher than 80 DEG C, the process energy consumption is big, and the surfactant containing phenols used has a bio-toxicity, and difficult degradation is residual in water body The time is stayed to grow, if phosphate therein is not handled thoroughly during liquid waste processing, P elements therein can largely carry out natural water, So as to cause the eutrophication problem of water body.

Zhang Xiaoming exists《The research of the without phosphorus degreasing powder of steel and iron parts》In one text by contrast test test it is different without phosphorus and The performance of sodium triphosphate, consider the performance such as wash-aid effect and the binding force to calcium ions and magnesium ions, it is organic to have selected silicon silicic acid Carboxylic acid polymeric modification thing is as builder, and to substitute phosphate builder, this builder is not phosphorous, fundamentally solves The processing of phosphorus and emission problem in waste liquid, while employ a kind of table outstanding as Material synthesis, environmental-protecting performance using plant oleyl alcohol Face activating agent-modification alcohol ethoxylate surfactant EH, as the key component of cleaning, this surfactant is relative For widely used primary alcohol ethoxylate and NPE, wetability and detergency are stronger, compound with the moon Ionic surface active agent and other nonionic surfactants, the efficiency of cleaning is improved, cleaning temperature is reduced to 60 DEG C, it is bright Aobvious reduces operating power consumption；And by the inspection in actual production, formula is optimized for actual electroplating enterprise In, there is the advantages of fast cleaning rate, efficiency high, there is preferable cleansing power to different greasy dirts, it is not necessary in disposing waste liquid Phosphate, simplify treatment process, reduce the usage amount of degreasing powder, reduce cost, reduce the COD of waste liquid, reduce The characteristic of intractability.Although the advantages of method provided in this article has environmental protection, cost is low and cleaning rate is fast etc., There is the alkali of higher concentration in degreasing powder, be not suitable for the pre-treatment of alloy, limit application field, and in use Substantial amounts of oily waste liquor is inevitably produced, greasy dirt can not be decomposed although the greasy dirt of steel surface can be cleaned, so being Solve the above problems, also need to make its further modification it to be provided simultaneously with oil removing and decompose greasy dirt function, and expand use Scope, to meet the cleaning of various section bars.

The content of the invention

The defects of the object of the invention is exactly to make up prior art, there is provided a kind of magnetic silica is fixed degradation bacteria and resisted Aoxidize compound powder cleaning agent and cleaning.

The present invention is achieved by the following technical solutions：

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, and system is prepared by the raw material of following parts by weight Into：Sodium hydroxide 13-15, sodium carbonate 22-25, metasilicate pentahydrate sodium 4-5, silicon silicic acid organic carboxyl acid polymeric modification thing 13-15, ten Dialkyl benzene sulfonic acids sodium 2.8-3.3, polyepoxy sodium succinate 3.6-4, coconut oil diethanol amine 1.2-1.5, isomery undecyl alcohol gather Oxygen vinethene 1.5-1.8, hexamethylene 32-36, poly- MTES 4.8-5.2, silane coupler kh5501.2-1.5, Nanometer magnetofluid 1.6-1.9, picoline 2.8-3.3, Sodium hydroxide q. s, 0.9% sodium chloride are appropriate, appropriate absolute ethyl alcohol, go Ion appropriate amount of water.

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, by following specific steps system Prepare into：

（1）Pedotheque will be gathered from the varying environment of oil pollution as bacterium source, and be added separately to be made in deionized water dense The soil liquid for 0.25g/ml is spent, is staticly settled after stirring 30min, 0.2g/ml is added after supernatant is mixed evenly The leaching liquor of uncontaminated rich soil, control temperature is at 30 DEG C, pH value 7.5, by colony inoculation to crude oil minimal medium Upper carry out enrichment culture, the degradation rate of oil is determined, choose the high bacterium of degradation rate as purpose strain；

（2）By step（1）Obtained purpose bacteria culture fluid carries out tamed strain, is exposed using crude oil minimal medium Gas domestication culture, it is 35 DEG C to control temperature, pH value 8.5, and the domestication of mixed liquor aeration determines residua content after 1 day, then quantitative 7# machine oil, Phos and inorganic nitrogen are supplemented, it is met carbon：Nitrogen：Phosphorus is 100:5:1, wherein carbon content is quality in domestication liquid The 1% of fraction, the bacterium colony that oil degradation speed is fast, colonial morphology is consistent with thalli morphology is retained as purebred；

（3）Nanometer magnetofluid is added in deionized water ultrasonic disperse into 1.6wt% dispersion liquids, then adds hexamethylene, poly- thereto MTES, silane coupler kh550 and picoline, the mixed liquor is emulsified with ultrasonic cell disruptor The finely dispersed liquid of system is obtained after 45min, regulation system pH value is 10-11, is centrifuged after being stored at room temperature 24h, solid Precipitation is washed for several times with absolute ethyl alcohol and deionized water, is subsequently placed at 80 DEG C and is dried in vacuo, it is standby to be cooled to room temperature；

（4）By step（2）The strain that aeration domestication obtains is inoculated into beef-protein medium, in 30 DEG C, 150r/min 3h is cultivated on constant temperature oscillator, each bacterial strain is in exponential phase, strain suspension is made, is inoculated into 5% inoculum concentration In 100ml beef-protein mediums, 1wt% steps are then added（3）The product of preparation, in 30 DEG C of rotating speeds be 160rpm Reaction 15-18h, is centrifuged off supernatant, lower floor is washed for several times with 0.9% sodium chloride solution, gained immobilized thallus in shaking table Natural drying at room temperature is standby；

（5）By sodium hydroxide, sodium carbonate, metasilicate pentahydrate sodium and step（4）The product of preparation is stirred 3-5min, adds Remaining remaining product stirring 5-10min, packaging can obtain compound powder cleaning agent.

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, the crude oil inorganic salts training Support base formula be：7# machine oil 0.2-0.3%, sodium chloride 0.5-0.6%, ammonium sulfate 0.1-0.15%, magnesium sulfate 0.025-0.03%, Sodium nitrate 0.2-0.25%, potassium dihydrogen phosphate 0.5-0.6%, dipotassium hydrogen phosphate 1.0-1.5%, agar 1.6-2%；The beef extract egg The formula of peptone culture medium is in vain：Beef extract 3-4g/L, peptone 10-11g/L, sodium chloride 5-5.5g/L, agar 15-16g/L, plus For deionized water to 1L, regulation pH is 7-7.2,121 DEG C of sterilizing 20min.

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, comprises the following steps：

（1）Compound powder cleaning agent is added to the cleaning fluid for being configured to that concentration is 55-65g/L in deionized water, is added to cleaning In groove, adjustment system pH is 11-12.5；

（2）Workpiece is placed in rinse bath, soaks 5-10min in 55-60 DEG C of underflow stream.

It is an advantage of the invention that：Metal is carrying out before processing, it is necessary to washes the dirts such as antirust oil, the cutting fluid on surface The processing of latter step can be carried out, although traditional phosphorus system cleaning agent with good cleaning performance, to the eutrophy of water body Change seriously substitute by without phosphorus powder cleaning agent gradually, but without phosphorus cleaning agent is alkaline too weak, it is difficult to wash grease, dirty Dirt, alkalescence then has very strong corrosivity very much to metal base by force, if excessive use surfactant containing phenols, although cleaning Effect is good, but has bio-toxicity, difficult degradation, and the residence time is grown in water body, and can not decompose greasy dirt, liquid waste processing difficulty Greatly, cost is high, for these problems present invention using strain sample is gathered from the environment of oil pollution, is carried out as bacterium source rich Collect domestication culture and filters out the high bacterial strain of degraded oil rate, there is magnetic material good biocompatibility, source extensively, easily to make The characteristics such as standby, hypotoxicity, have a wide range of applications, and herein using hexamethylene as oil phase, deionized water is aqueous phase, and magnetic fluid is profit The emulsion of interface stability particle, poly- MTES synthesize magnetic silica tiny balloon as silicon source, tool There is the advantages of excellent damping and amortization, heat endurance, chemical stability and high absorption property, be fixed degraded by suction-operated Bacterium, and carry out compounding with the non-phosphorus builder that filters out, surfactant etc. and compound powder cleaning agent is made, can not only The greasy dirt on cleaning workpiece surface, there is decomposition, catalytic action well to oily waste water, reduce COD value, reduce the place of waste water Manage difficulty and processing cost, and the degradation bacteria fixed of magnetic carrier can magnetic recovery utilize, reduce cost, prepared by the present invention Powder cleaning agent it is not phosphorous, environmentally friendly, cleaning performance is more held better than conventional pre-treatment oil removing process, service life Long, it is nontoxic to environment, use manpower and material resources sparingly, use range is wide, and caused waste water is more disposable.

Embodiment

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, by following parts by weight（Kilogram）'s Raw material is made：Sodium hydroxide 13, sodium carbonate 22, metasilicate pentahydrate sodium 4, silicon silicic acid organic carboxyl acid polymeric modification thing 13, ten Dialkyl benzene sulfonic acids sodium 2.8, polyepoxy sodium succinate 3.6, coconut oil diethanol amine 1.2, isomery undecyl alcohol APEO 1.5th, hexamethylene 32, poly- MTES 4.8, silane coupler kh5501.2, nanometer magnetofluid 1.6, picoline 2.8th, Sodium hydroxide q. s, 0.9% sodium chloride is appropriate, appropriate absolute ethyl alcohol, appropriate deionized water.

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, by following specific steps system Prepare into：

（1）Pedotheque will be gathered from the varying environment of oil pollution as bacterium source, and be added separately to be made in deionized water dense The soil liquid for 0.25g/ml is spent, is staticly settled after stirring 30min, 0.2g/ml is added after supernatant is mixed evenly The leaching liquor of uncontaminated rich soil, control temperature is at 30 DEG C, pH value 7.5, by colony inoculation to crude oil minimal medium Upper carry out enrichment culture, the degradation rate of oil is determined, choose the high bacterium of degradation rate as purpose strain；

（2）By step（1）Obtained purpose bacteria culture fluid carries out tamed strain, is exposed using crude oil minimal medium Gas domestication culture, it is 35 DEG C to control temperature, pH value 8.5, and the domestication of mixed liquor aeration determines residua content after 1 day, then quantitative 7# machine oil, Phos and inorganic nitrogen are supplemented, it is met carbon：Nitrogen：Phosphorus is 100:5:1, wherein carbon content is quality in domestication liquid The 1% of fraction, the bacterium colony that oil degradation speed is fast, colonial morphology is consistent with thalli morphology is retained as purebred；

（3）Nanometer magnetofluid is added in deionized water ultrasonic disperse into 1.6wt% dispersion liquids, then adds hexamethylene, poly- thereto MTES, silane coupler kh550 and picoline, the mixed liquor is emulsified with ultrasonic cell disruptor The finely dispersed liquid of system is obtained after 45min, regulation system pH value is 10, is centrifuged after being stored at room temperature 24h, solid precipitation Washed for several times with absolute ethyl alcohol and deionized water, be subsequently placed at 80 DEG C and be dried in vacuo, it is standby to be cooled to room temperature；

（4）By step（2）The strain that aeration domestication obtains is inoculated into beef-protein medium, in 30 DEG C, 150r/min 3h is cultivated on constant temperature oscillator, each bacterial strain is in exponential phase, strain suspension is made, is inoculated into 5% inoculum concentration In 100ml beef-protein mediums, 1wt% steps are then added（3）The product of preparation, in 30 DEG C of rotating speeds be 160rpm Reaction 15h, is centrifuged off supernatant, lower floor is washed for several times with 0.9% sodium chloride solution, gained immobilized thallus room in shaking table Temperature spontaneously dries standby；

（5）By sodium hydroxide, sodium carbonate, metasilicate pentahydrate sodium and step（4）The product of preparation is stirred 3min, adds it Remaining remaining product stirring 5min, packaging can obtain compound powder cleaning agent.

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, the crude oil inorganic salts training Support base formula be：7# machine oil 0.2%, sodium chloride 0.5%, ammonium sulfate 0.1%, magnesium sulfate 0.025%, sodium nitrate 0.2%, di(2-ethylhexyl)phosphate Hydrogen potassium 0.5%, dipotassium hydrogen phosphate 1.0%, agar 1.6%；The formula of the beef-protein medium is：Beef extract 3g/L, egg To 1L, regulation pH is 7,121 DEG C of sterilizing 20min for white peptone 10g/L, sodium chloride 5g/L, agar 15g/L plus deionized water.

A kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, comprises the following steps：

（1）Compound powder cleaning agent is added to the cleaning fluid for being configured to that concentration is 55g/L in deionized water, is added to rinse bath In, adjustment system pH is 11；

（2）Workpiece is placed in rinse bath, soaks 5min in 55 DEG C of underflow streams.

The without phosphorus powder cleaning agent prepared to embodiment is configured to cleaning fluid, and 45# steel and LY12 aluminium alloys are impregnated respectively 5min is cleaned, takes out observation：Surface decoction clarification, illustrates that the powder cleaning agent has Rapid Cleaning and decomposes greasy dirt without oil bloom The effect of.

Claims (4)

1. a kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, it is characterised in that by following weight The raw material of part is made：Sodium hydroxide 13-15, sodium carbonate 22-25, metasilicate pentahydrate sodium 4-5, the polymerization of silicon silicic acid organic carboxyl acid Modifier 13-15, neopelex 2.8-3.3, polyepoxy sodium succinate 3.6-4, coconut oil diethanol amine 1.2- 1.5th, isomery undecyl alcohol APEO 1.5-1.8, hexamethylene 32-36, poly- MTES 4.8-5.2, silane are even Connection agent kh5501.2-1.5, nanometer magnetofluid 1.6-1.9, picoline 2.8-3.3, Sodium hydroxide q. s, 0.9% sodium chloride are fitted Amount, appropriate absolute ethyl alcohol, appropriate deionized water.

2. a kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria according to claim 1, it is special Sign is, is made up of following specific steps：

（1）Pedotheque will be gathered from the varying environment of oil pollution as bacterium source, and be added separately to be made in deionized water dense The soil liquid for 0.25g/ml is spent, is staticly settled after stirring 30min, 0.2g/ml is added after supernatant is mixed evenly The leaching liquor of uncontaminated rich soil, control temperature is at 30 DEG C, pH value 7.5, by colony inoculation to crude oil minimal medium Upper carry out enrichment culture, the degradation rate of oil is determined, choose the high bacterium of degradation rate as purpose strain；

（2）By step（1）Obtained purpose bacteria culture fluid carries out tamed strain, is exposed using crude oil minimal medium Gas domestication culture, it is 35 DEG C to control temperature, pH value 8.5, and the domestication of mixed liquor aeration determines residua content after 1 day, then quantitative 7# machine oil, Phos and inorganic nitrogen are supplemented, it is met carbon：Nitrogen：Phosphorus is 100:5:1, wherein carbon content is quality in domestication liquid The 1% of fraction, the bacterium colony that oil degradation speed is fast, colonial morphology is consistent with thalli morphology is retained as purebred；

（3）Nanometer magnetofluid is added in deionized water ultrasonic disperse into 1.6wt% dispersion liquids, then adds hexamethylene, poly- thereto MTES, silane coupler kh550 and picoline, the mixed liquor is emulsified with ultrasonic cell disruptor The finely dispersed liquid of system is obtained after 45min, regulation system pH value is 10-11, is centrifuged after being stored at room temperature 24h, solid Precipitation is washed for several times with absolute ethyl alcohol and deionized water, is subsequently placed at 80 DEG C and is dried in vacuo, it is standby to be cooled to room temperature；

（4）By step（2）The strain that aeration domestication obtains is inoculated into beef-protein medium, in 30 DEG C, 150r/min 3h is cultivated on constant temperature oscillator, each bacterial strain is in exponential phase, strain suspension is made, is inoculated into 5% inoculum concentration In 100ml beef-protein mediums, 1wt% steps are then added（3）The product of preparation, in 30 DEG C of rotating speeds be 160rpm Reaction 15-18h, is centrifuged off supernatant, lower floor is washed for several times with 0.9% sodium chloride solution, gained immobilized thallus in shaking table Natural drying at room temperature is standby；

（5）By sodium hydroxide, sodium carbonate, metasilicate pentahydrate sodium and step（4）The product of preparation is stirred 3-5min, adds Remaining remaining product stirring 5-10min, packaging can obtain compound powder cleaning agent.

3. a kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria according to claim 2, it is special Sign is that the formula of the crude oil minimal medium is：7# machine oil 0.2-0.3%, sodium chloride 0.5-0.6%, ammonium sulfate 0.1- 0.15%th, magnesium sulfate 0.025-0.03%, sodium nitrate 0.2-0.25%, potassium dihydrogen phosphate 0.5-0.6%, dipotassium hydrogen phosphate 1.0- 1.5%th, agar 1.6-2%；The formula of the beef-protein medium is：Beef extract 3-4g/L, peptone 10-11g/L, chlorine Change sodium 5-5.5g/L, agar 15-16g/L plus deionized water to 1L, regulation pH is 7-7.2,121 DEG C of sterilizing 20min.

4. a kind of magnetic silica fixes the anti-oxidant compound powder cleaning agent of degradation bacteria, it is characterised in that including following step Suddenly：

（1）Compound powder cleaning agent is added to the cleaning fluid for being configured to that concentration is 55-65g/L in deionized water, is added to cleaning In groove, adjustment system pH is 11-12.5；

（2）Workpiece is placed in rinse bath, soaks 5-10min in 55-60 DEG C of underflow stream.