CN107353398B - A kind of water solubility unsaturated aliphatic polyester and its preparation method and application - Google Patents
A kind of water solubility unsaturated aliphatic polyester and its preparation method and application Download PDFInfo
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- CN107353398B CN107353398B CN201710667509.5A CN201710667509A CN107353398B CN 107353398 B CN107353398 B CN 107353398B CN 201710667509 A CN201710667509 A CN 201710667509A CN 107353398 B CN107353398 B CN 107353398B
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Abstract
The present invention relates to water-soluble unsaturated aliphatic polyester, which is the linearly aligned polyester of structural unit shown in chemical formula (I), number-average molecular weight (abbreviation Mn) it is 2000~5000, monodispersity index (abbreviation PDI) is 1.54~1.89；The water-soluble unsaturated aliphatic polyester is that 2,3- epoxypropyltrimethylchloride chloride and maleic anhydride are carried out ring opening copolymer to obtain.The hydrogel of unsaturated aliphatic polyester preparation of the present invention has preferable elasticity modulus and swelling behavior, hydrophily and anti-microbial property.
Description
Technical field
The present invention relates to organic high molecular compounds, and in particular to unsaturated aliphatic polyester, the unsaturated fat adoption
Ester is suitable for medical material.
Background technique
Its main chain of aliphatic polyester is formed by connecting by aliphatic structure unit by the ester bond of facile hydrolysis, and main chain is submissive,
It is easy under the action of microorganism, is degraded by the catalysis of enzyme.Therefore, aliphatic polyester is because it is with good life
Object compatibility and biodegradability are a kind of important bio-medical materials, are widely used in biomedicine field, such as drug
Sustained release, operation suture thread, bone immobilizing material, tissue engineering material etc..Common aliphatic polyester has polyglycolide (PGA), gathers
Lactide (PLA), glycolide-lactide copolymer (PLGA), polycaprolactone (PCL) etc..The synthesis side of aliphatic polyester at present
There are two types of methods.First is to be prepared using bifunctional monomer by condensation, such as be condensed and gathered with binary acid and dihydric alcohol
Conjunction obtains.Second is to use its ester compounds or lactone compound for monomer, carries out ring opening copolymer and obtains.But aliphatic poly
Ester is there are several biggish defects, and first, the hydrophily of aliphatic polyester is poor, bioactivity is low, affinity to cell and viscous
Attached power is weak.The main chain of second aliphatic polyester lacks reactivity site, limits the application of aliphatic polyester.
Unsaturated aliphatic polyester refers to the aliphatic polyester containing unsaturated double-bond, can be with the monomer containing unsaturated double-bond
Or prepolymer is solidified.Currently, the preparation method of unsaturated aliphatic polyester mainly uses condensation methods.Condensation methods be exactly by
Unsaturated dibasic aliphatic acid or dibasic aliphatic acid anhydrides are obtained with unsaturated dihydric alcohol polycondensation.The insatiable hunger of this method preparation
Contain unsaturated double-bond with aliphatic polyester main chain, can with containing unsaturated double-bond monomer or prepolymer be crosslinked it is solid
Change.Yang Qun etc. has synthesized insatiable hunger using fumaric acid, diglycol and 1,4-butanediol as raw material, using melt-polycondensation
With aliphatic polyester and copolyesters, find the unsaturated aliphatic polyester have good biodegradability (using chemical industry,
2013, the 3rd phase of volume 42,490).But the hydrophily of above-mentioned unsaturated aliphatic polyester is poor, anti-microbial property is low, Bu Nengman
Foot prepares the requirement of tissue engineering bracket.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of water-soluble unsaturated aliphatic polyester, the unsaturated aliphatics
Contain unsaturated double-bond on polyester backbone, contain quaternary ammonium salt on side chain, there is good hydrophily and antibacterial effect.
Technical proposal that the invention solves the above-mentioned problems is:
A kind of water solubility unsaturated aliphatic polyester, which is that structural unit shown in chemical formula (I) is linearly aligned
Polyester, number-average molecular weight (abbreviation Mn) it is 2000~5000, monodispersity index (abbreviation PDI) is 1.54~1.89,
The water-soluble unsaturated aliphatic polyester is by 2,3- epoxypropyltrimethylchloride chloride and maleic acid
Acid anhydride carries out ring opening copolymer and obtains.
Above-mentioned water solubility unsaturated aliphatic polyester specific the preparation method is as follows:
Maleic anhydride and 2 is measured by equimolar, it is molten that suitable mixing is added in 3- epoxypropyltrimethylchloride chloride
Liquid, under nitrogen protection, 80~110 DEG C of ring opening copolymers react 3~6h, remove solvent and obtain the water-soluble unsaturated fat
Adoption ester；The mixed solution is made of ethyl acetate and toluene, and wherein the volume ratio of Yi Suan Yi Zhi ︰ toluene is 1 ︰ 4.
In the above method, the reaction temperature is preferably 100 DEG C；Reaction time is preferably 4h.
Unsaturated aliphatic polyester of the present invention contains unsaturated double-bond on main chain, can with acrylic monomers into
Row solidification, is used to prepare the anti-bacterial hydrogel of good hydrophilic property.
A kind of anti-bacterial hydrogel, the hydrogel is by unsaturated aliphatic polyester, hydrophily unsaturated monomer, soft unsaturation
Monomer and methyl ethyl ketone peroxide composition, wherein
The content of the hydrophily unsaturated monomer is the 40~60% of unsaturated aliphatic polyester weight；
The content of the soft unsaturated monomer is the 40~60% of unsaturated aliphatic polyester weight；
The content of the methyl ethyl ketone peroxide is the 0.5~1% of unsaturated aliphatic polyester weight；
The hydrophily unsaturated monomer is hydroxypropyl acrylate, hydroxy propyl methacrylate, hydroxyethyl methacrylate second
Ester, hydroxy-ethyl acrylate and acrylic acid-N, one of TMSDMA N dimethylamine base ethyl ester or two or more；
The soft unsaturated monomer is in butyl acrylate, acrylic acid-2-ethyl caproite and Isooctyl acrylate monomer
More than one or two kinds of.
In anti-bacterial hydrogel of the present invention, the content of hydrophily unsaturated monomer is preferably unsaturated aliphatic polyester
The 50% of weight.
In anti-bacterial hydrogel of the present invention, the content of soft unsaturated monomer is preferably unsaturated aliphatic polyester weight
The 50% of amount.
Anti-bacterial hydrogel of the present invention heats 5~10min at 60~80 DEG C can be solidified into required tissue
Engineering rack.
Unsaturated aliphatic polyester of the present invention and hydrophily unsaturated monomer, under methyl ethyl ketone peroxide effect,
It is that double bond reacts and can be formed on unsaturated double-bond and hydrophily unsaturated monomer on unsaturated aliphatic polyester main chain
Hydrogel.The hydrogel has the advantages that preferable elasticity modulus and swelling behavior.Meanwhile unsaturated aliphatic of the present invention
Polyester side chains contain quaternary ammonium salt, therefore have good hydrophily and anti-microbial property with its hydrogel obtained, can be used for preparing
The tissue engineering bracket of antibacterial.
Specific embodiment
Preparation method and its effect of the invention are described in further detail with specific embodiment below.
Embodiment 1
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol,
15.1g), it is added to 100mL ethyl acetate: in toluene=1:1 volume ratio mixed solution, under nitrogen protection, 110 DEG C of open loops
Copolyreaction 6h removes solvent and obtains unsaturated aliphatic polyester.
The weight average molecular weight and number-average molecular weight of unsaturated aliphatic polyester use Jasco Gulliver system (PU-
980, CO-965, RI-930, and UV-1570) gel permeation chromatograph is measured.It is equipped with Aquapak A-440 column
(Shodex column s K804, K805, and J806) uses THF as eluant, eluent, and polystyrene is as standard correction, and 30
It is measured at DEG C.Testing result are as follows: Mw=9467, Mn=5009, PDI=1.89.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g, hydroxypropyl acrylate 1g, hydroxyethyl methacrylate 1g prepared by step 1, propylene
Acid butyl ester 1g, Isooctyl acrylate monomer 1g, methyl ethyl ketone peroxide 0.025g.
(b) preparation of hydrophilic antibiotic hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) hydrophilic antibiotic hydrogel performance
For the performance for verifying hydrophilic antibiotic hydrogel of the present invention, above-mentioned hydrophilic antibiotic hydrogel is perfused first
Into the mold of tissue engineering bracket, the mold is then heated into 5min at 80 DEG C, the group weaver of antibacterial is obtained after demoulding
Engineering support.
1. swelling behavior
The antibacterial bracket sample of preparation, which is placed in drying in 60 DEG C of baking oven, for 24 hours, dehydrates it sufficiently, and it is dry to measure it
The weight of antibacterial bracket, is then soaked in deionized water at room temperature, reaches swelling equilibrium to it, wipes antibacterial with filter paper
The extra moisture of rack surface measures the quality of antibacterial bracket again, finally calculates swelling ratio.
It is detected according to the above method, the swelling ratio of prepared antibacterial bracket is 287%.
2. elasticity modulus
By on the antibacterial bracket sample of preparation by 2202 universal testing machine of Zwick/Roell (German Zwick company)
Detect its elasticity modulus.
It is detected according to the above method, the elasticity modulus of prepared antibacterial bracket is 1586Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.22mm, golden yellow
Staphylococcic bacteriostatic diameter is 13.45mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 2
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
It is added to 100mL ethyl acetate: in toluene=1:4 volume ratio mixed solution, under nitrogen protection, 80 DEG C of ring opening copolymer reactions
3h removes solvent and obtains unsaturated aliphatic polyester.
Gained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=3077, Mn
=1998, PDI=1.54.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g prepared by step 1, hydroxy propyl methacrylate 0.5g, hydroxyethyl methacrylate
0.5g, hydroxy-ethyl acrylate 0.8g, acrylic acid-N, TMSDMA N dimethylamine base ethyl ester 1.2g, butyl acrylate 1g, acrylic acid-2-ethyl
Own ester 2g, methyl ethyl ketone peroxide 0.025g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into transparent tissue first
In the mold of engineering rack, the mold is then heated into 8min at 75 DEG C, antibacterial tissue engineering bracket is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 326%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 1688Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.34mm, golden yellow
Staphylococcic bacteriostatic diameter is 13.86mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 3
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
It is added to 100mL ethyl acetate: in toluene=1:1 volume ratio mixed solution, under nitrogen protection, 90 DEG C of ring opening copolymer reactions
5h removes solvent and obtains unsaturated aliphatic polyester.
Obtained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=
3579,Mn=2156, PDI=1.66.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g prepared by step 1, hydroxyethyl methacrylate 2g, butyl acrylate 1g acrylic acid are different
Monooctyl ester 1g, methyl ethyl ketone peroxide 0.04g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into organizational project branch first
In the mold of frame, the mold is then heated into 10min at 60 DEG C, the tissue engineering bracket of antibacterial is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 382%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 2057Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.64mm, golden yellow
Staphylococcic bacteriostatic diameter is 12.96mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 4
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
Be added to 100mL ethyl acetate: in toluene=1:4 volume ratio mixed solution, under nitrogen protection, 100 DEG C of ring opening copolymers are anti-
4h is answered, solvent is removed and obtains unsaturated aliphatic polyester.
Obtained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=
5738,Mn=3336, PDI=1.723.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g, acrylic acid-N, TMSDMA N dimethylamine base ethyl ester 1.5g prepared by step 1, five polyethylene glycol
Methyl ether acetate 1.0g, acrylic acid-2-ethyl caproite 2.4g, methyl ethyl ketone peroxide 0.03g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into organizational project branch first
In the mold of frame, the mold is then heated into 6min at 75 DEG C, the tissue engineering bracket of antibacterial is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 305%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 3150Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 7.96mm, golden yellow
Staphylococcic bacteriostatic diameter is 12.87mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 5
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
Be added to 100mL ethyl acetate: in toluene=1:4 volume ratio mixed solution, under nitrogen protection, 100 DEG C of ring opening copolymers are anti-
6h is answered, solvent is removed and obtains unsaturated aliphatic polyester.
Obtained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=
7555,Mn=4367, PDI=1.73.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g prepared by step 1, hydroxy propyl methacrylate 2.2g, acrylic acid-2-ethyl caproite
2.8g, methyl ethyl ketone peroxide 0.05g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into organizational project branch first
In the mold of frame, the mold is then heated into 9min at 65 DEG C, the tissue engineering bracket of antibacterial is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 291%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 2888Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.61mm, golden yellow
Staphylococcic bacteriostatic diameter is 14.47mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 6
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
It is added to 100mL ethyl acetate: in toluene=1:4 volume ratio mixed solution, under nitrogen protection, 80 DEG C of ring opening copolymer reactions
4h removes solvent and obtains unsaturated aliphatic polyester.
Obtained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=
3552,Mn=2234, PDI=1.59.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g prepared by step 1, hydroxyethyl methacrylate 0.2g, hydroxy propyl methacrylate
0.8g, hydroxy-ethyl acrylate 1g, acrylic acid-N, TMSDMA N dimethylamine base ethyl ester 0.6g, acrylic acid-2-ethyl caproite 2.3g, peroxidating
Methyl ethyl ketone 0.035g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into organizational project branch first
In the mold of frame, the mold is then heated into 6.5min at 75 DEG C, the tissue engineering bracket of antibacterial is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 116%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 986Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.36mm, golden yellow
Staphylococcic bacteriostatic diameter is 13.55mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 7
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
Be added to 100mL ethyl acetate: in toluene=1:4 volume ratio mixed solution, under nitrogen protection, 100 DEG C of ring opening copolymers are anti-
5h is answered, solvent is removed and obtains unsaturated aliphatic polyester.
Obtained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=
7031,Mn=4064, PDI=1.73.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g prepared by step 1, hydroxy propyl methacrylate 2.5g, butyl acrylate 1.2g, third
Olefin(e) acid -2- ethylhexyl 0.6g, Isooctyl acrylate monomer 1.0g, methyl ethyl ketone peroxide 0.044g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into organizational project branch first
In the mold of frame, the mold is then heated into 6.2min at 76 DEG C, the tissue engineering bracket of antibacterial is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 257%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 1998Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.62mm, golden yellow
Staphylococcic bacteriostatic diameter is 13.13mm.
The above results show that antibacterial bracket has good antibacterial effect.
Embodiment 8
(1) preparation of unsaturated aliphatic polyester
Take maleic anhydride (9.8g, 100mmol) and 2,3- epoxypropyltrimethylchloride chloride (100mmol, 15.1g)
Be added to 100mL ethyl acetate: in toluene=1:1 volume ratio mixed solution, under nitrogen protection, 100 DEG C of ring opening copolymers are anti-
3h is answered, solvent is removed and obtains unsaturated aliphatic polyester.
Obtained unsaturated aliphatic polyester is detected by 1 the method for embodiment, testing result are as follows: Mw=
6418,Mn=3962, PDI=1.62.
(2) preparation and its performance of anti-bacterial hydrogel
(a) formula of anti-bacterial hydrogel
Unsaturated aliphatic polyester 5g, hydroxy propyl methacrylate 2.5g, Isooctyl acrylate monomer 2.5g prepared by step 1,
Methyl ethyl ketone peroxide 0.032g.
(b) preparation of anti-bacterial hydrogel
Above-mentioned steps (a) raw material is uniformly mixed to get the hydrophilic antibiotic hydrogel.
(c) anti-bacterial hydrogel performance
For the performance for verifying anti-bacterial hydrogel of the present invention, above-mentioned anti-bacterial hydrogel is filled into organizational project branch first
In the mold of frame, the mold is then heated into 7min at 70 DEG C, the tissue engineering bracket of antibacterial is obtained after demoulding.
1. swelling behavior
It is detected by 1 the method for embodiment, the swelling ratio of prepared antibacterial bracket is 232%.
2. elasticity modulus
It is detected by 1 the method for embodiment, the elasticity modulus of prepared antibacterial bracket is 2063Pa.
3. antibacterial experiment
According to QB/T2591-2003 standard, gram-positive bacteria-staphylococcus aureus is carried out to antibacterial bracket and leather is blue
The test of family name's negative bacterium-Escherichia coli bacteriostasis property.The bacteriostatic diameter of antibacterial Escherichia coli as the result is shown is 8.76mm, golden yellow
Staphylococcic bacteriostatic diameter is 13.55mm.
The above results show that antibacterial bracket has good antibacterial effect.
Claims (4)
1. a kind of water solubility unsaturated aliphatic polyester, which is that structural unit shown in chemical formula (I) is linearly aligned poly-
Ester, number-average molecular weight are 2000~5000, and monodispersity index is 1.54~1.89,
The water-soluble unsaturated aliphatic polyester be by 2,3- epoxypropyltrimethylchloride chloride and maleic anhydride into
Row ring opening copolymer obtains.
2. water solubility unsaturated aliphatic polyester described in claim 1, which is characterized in that the water solubility unsaturated fat adoption
Ester is made by following methods:
Maleic anhydride and 2 are taken by equimolar ratio, suitable mixed solution is added in 3- epoxypropyltrimethylchloride chloride,
Under nitrogen protection, 80~110 DEG C of ring opening copolymers react 3~6h, remove solvent and obtain the water-soluble unsaturated fat adoption
Ester；The mixed solution is made of ethyl acetate and toluene, and wherein the volume ratio of Yi Suan Yi Zhi ︰ toluene is 1 ︰ 4.
3. a kind of anti-bacterial hydrogel, the hydrogel is by water-soluble unsaturated aliphatic polyester of any of claims 1 or 2, hydrophilic
Property unsaturated monomer, soft unsaturated monomer and methyl ethyl ketone peroxide composition, wherein
The content of the hydrophily unsaturated monomer is the 40~60% of water-soluble unsaturated aliphatic polyester weight；
The content of the soft unsaturated monomer is the 40~60% of water-soluble unsaturated aliphatic polyester weight；
The content of the methyl ethyl ketone peroxide is the 0.5~1% of unsaturated aliphatic polyester weight；
The hydrophily unsaturated monomer is hydroxypropyl acrylate, hydroxy propyl methacrylate, hydroxyethyl methacrylate, third
Olefin(e) acid hydroxyl ethyl ester and acrylic acid-N, one of TMSDMA N dimethylamine base ethyl ester or two or more；
The soft unsaturated monomer is butyl acrylate or Isooctyl acrylate monomer.
4. application of the anti-bacterial hydrogel as claimed in claim 3 in the tissue engineering bracket of preparation antibacterial.
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