CN107347796B - A kind of breeding method of blackhead not holding any government official post mutton sheep new lines - Google Patents
A kind of breeding method of blackhead not holding any government official post mutton sheep new lines Download PDFInfo
- Publication number
- CN107347796B CN107347796B CN201710618155.5A CN201710618155A CN107347796B CN 107347796 B CN107347796 B CN 107347796B CN 201710618155 A CN201710618155 A CN 201710618155A CN 107347796 B CN107347796 B CN 107347796B
- Authority
- CN
- China
- Prior art keywords
- breeding
- goat
- generation
- gene
- color
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 82
- 241001494479 Pecora Species 0.000 title claims abstract description 52
- 206010000496 acne Diseases 0.000 title claims abstract description 22
- 241000283707 Capra Species 0.000 claims abstract description 107
- 230000001488 breeding effect Effects 0.000 claims abstract description 64
- 230000037308 hair color Effects 0.000 claims abstract description 44
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 39
- 235000013372 meat Nutrition 0.000 claims abstract description 25
- 238000012360 testing method Methods 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000005516 engineering process Methods 0.000 claims abstract description 16
- 238000006116 polymerization reaction Methods 0.000 claims abstract description 13
- 230000002068 genetic effect Effects 0.000 claims description 17
- 239000008280 blood Substances 0.000 claims description 14
- 210000004369 blood Anatomy 0.000 claims description 14
- 230000012010 growth Effects 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- 244000144992 flock Species 0.000 claims description 11
- 210000002268 wool Anatomy 0.000 claims description 11
- 238000011161 development Methods 0.000 claims description 10
- 108700028369 Alleles Proteins 0.000 claims description 9
- 238000009396 hybridization Methods 0.000 claims description 9
- 230000009897 systematic effect Effects 0.000 claims description 7
- 101150015730 Prlr gene Proteins 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 210000004209 hair Anatomy 0.000 claims description 6
- 238000011835 investigation Methods 0.000 claims description 6
- 101150032511 SKIP gene Proteins 0.000 claims description 5
- 101150015860 MC1R gene Proteins 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 206010020112 Hirsutism Diseases 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000012252 genetic analysis Methods 0.000 claims description 3
- 230000008303 genetic mechanism Effects 0.000 claims description 3
- 230000008774 maternal effect Effects 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- 208000035240 Disease Resistance Diseases 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims 1
- 230000001850 reproductive effect Effects 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 239000003147 molecular marker Substances 0.000 abstract description 4
- 230000006641 stabilisation Effects 0.000 abstract description 3
- 238000011105 stabilization Methods 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 abstract description 2
- 230000018109 developmental process Effects 0.000 description 8
- 235000014113 dietary fatty acids Nutrition 0.000 description 8
- 229930195729 fatty acid Natural products 0.000 description 8
- 239000000194 fatty acid Substances 0.000 description 8
- 150000004665 fatty acids Chemical class 0.000 description 8
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 6
- 101000825204 Homo sapiens A-kinase anchor protein SPHKAP Proteins 0.000 description 5
- 101001053320 Homo sapiens Inositol polyphosphate 5-phosphatase K Proteins 0.000 description 5
- 101000836279 Homo sapiens SNW domain-containing protein 1 Proteins 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 210000000038 chest Anatomy 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 5
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 102100022252 A-kinase anchor protein SPHKAP Human genes 0.000 description 4
- 235000019687 Lamb Nutrition 0.000 description 4
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 4
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 4
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 4
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 description 3
- 235000021355 Stearic acid Nutrition 0.000 description 3
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 235000013922 glutamic acid Nutrition 0.000 description 3
- 239000004220 glutamic acid Substances 0.000 description 3
- 210000000003 hoof Anatomy 0.000 description 3
- 235000020778 linoleic acid Nutrition 0.000 description 3
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 3
- 238000005204 segregation Methods 0.000 description 3
- 239000008117 stearic acid Substances 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- 101001123448 Homo sapiens Prolactin receptor Proteins 0.000 description 2
- 238000007476 Maximum Likelihood Methods 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- 102100029000 Prolactin receptor Human genes 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 210000003128 head Anatomy 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 229960004488 linolenic acid Drugs 0.000 description 2
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 210000002445 nipple Anatomy 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- SECPZKHBENQXJG-FPLPWBNLSA-N palmitoleic acid Chemical compound CCCCCC\C=C/CCCCCCCC(O)=O SECPZKHBENQXJG-FPLPWBNLSA-N 0.000 description 2
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 description 1
- 241000272496 Galliformes Species 0.000 description 1
- 206010071602 Genetic polymorphism Diseases 0.000 description 1
- 101001134060 Homo sapiens Melanocyte-stimulating hormone receptor Proteins 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 102100034216 Melanocyte-stimulating hormone receptor Human genes 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 235000021360 Myristic acid Nutrition 0.000 description 1
- 241001416149 Ovis ammon Species 0.000 description 1
- 235000021319 Palmitoleic acid Nutrition 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 101150036080 at gene Proteins 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 208000021024 autosomal recessive inheritance Diseases 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- SECPZKHBENQXJG-UHFFFAOYSA-N cis-palmitoleic acid Natural products CCCCCCC=CCCCCCCCC(O)=O SECPZKHBENQXJG-UHFFFAOYSA-N 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 125000005313 fatty acid group Chemical group 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000005772 leucine Nutrition 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 235000018977 lysine Nutrition 0.000 description 1
- 230000008176 mammary development Effects 0.000 description 1
- 239000004579 marble Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
If this invention provides a kind of breeding method of blackhead not holding any government official post mutton sheep new lines, it is characterized by: using boer goat as male parent, Macheng black goat is female parent, using BLUP method, molecular marker assisted selection MAS technology and combine conventional breeding methods, fixed by traversed by, numerous means are expanded in embryo transfer, finally select that breeding potential is high, Meat Performance is good and the meat goat new lines of high-temp resisting high-humidity resisting.Breeding method of the invention is female parent with Macheng black goat, boer goat is male parent, using BLUP method, multiple gene polymerization assistant breeding technology and combine conventional breeding methods, cultivate the meat goat new lines for taking into account hair color, high reproductive rate, Meat Performance and energy high-temp resisting high-humidity resisting, there is blackhead not holding any government official post's feature in stabilization, Macheng black goat is significantly better than in terms of growing with meat production, reproductive performance, resistance to crude feed, it is disease-resistant in terms of be better than boer goat, the production performance for having reached modern naked eyed test is horizontal.
Description
Technical field
The present invention relates to a kind of breeding methods of black goat strain, belong to animal breeding technology field.
Background technique
Macheng black goat is one of excellent local Black Hills sheep variety of China, has that coat is completely black, precocious, reproductive capacity is strong, meat
The features such as matter is good.It is mainly distributed on the Dabie Mountains Region of Hubei, Henan, Anhui boundary, is more concentrated with Macheng, hubei Province area.
It is authorized by Hubei Province's livestock and poultry species validation board within 2002, is incorporated into " Hubei Province's domestic animals and fowls kind will " (2004 editions), at
For the excellent black goat local varieties in Hubei Province.In January, 2010 is selected in " national Genetic Resources of Domestic Animal kind catalogue ", becomes national
Excellent Black Hills sheep variety.Macheng black goat is without systematic breeding, and the local varieties of self-assembling formation are wanted away from meat goat kind
It asks, still there is larger gap, due to lacking systematic breeding, extensive feeding, microcommunity close relative breeding for a long time, cause black goat raw
Length is slow, it is long to deliver the period for sale, the variation of group's production performance is big, seriously constrains the development of black goat industry, is badly in need of new development
Direction.Boer goat is specialized meat goat kind generally acknowledged in the world, and boer goat incidence is brown (red) color, in volume
There is a white hair-videotape to nose, body body coat is entirely white, and it introduces boer goat before this and Macheng black goat carried out crossbreeding and improvement, though
The speed of growth so is improved, but is still had several drawbacks, first is that hybrid sheep adaptability is poor；Second is that color inheritance is unstable,
There is hair color segregation phenomenon with Macheng black goat filial generation, the more difficult stabilization of hair color, existing breeding method is also undesirable.Cultivate meat
Sheep new varieties (are) for pushing China's mutton sheep production system to build, to meet the needs of producers and consumers, produce to optimization
Structure, develops that safe, high-quality, efficient, section consumption, environmentally friendly Modern Animal Husbandry etc. plays a significant role and far reaching significance.
Chinese patent Authorization Notice No. CN 104017808 discloses a kind of genetic marker relevant to goat growth trait
And application, but for how to cultivate mutton sheep new varieties (being), have no specific selection.
Chinese Patent Application No. is 2012102829867, discloses the breeding method of Pu'an black goat strain, the cultivation
Method carries out two-way cross using construction of Guizhou black goat basis ewe and Nubia ram, selects that outstanding to exert black first-filial generation excellent
Elegant ewe makees maternal, selects outstanding construction of Guizhou black goat ram to make male parent and implements backcrossing, select the outstanding ram of backcross progeny with
Outstanding ewe carries out traversed by and fixes, and cultivates the Pu'an black goat strain relatively stable at gene.After it does not solve goat hybridization
, there is hair color segregation phenomenon, the more difficult stable technical problem of hair color in generation.
Summary of the invention
The purpose of the present invention is in order to overcome the deficiencies of the prior art using boer goat as male parent, Macheng black goat is female parent,
Using BLUP method, molecular marker assisted selection MAS technology and conventional breeding methods are combined, are fixed by traversed by, embryo transfer is expanded
Numerous means, finally select that breeding potential is high, Meat Performance is good and the meat goat new lines of high-temp resisting high-humidity resisting, blackhead not holding any government official post.
The technical scheme is that
A kind of breeding method of blackhead not holding any government official post mutton sheep new lines, comprising the following steps:
(1) boer goat and Macheng black goat are identified and is assessed using conventional selection combination BLUP method, selected excellent
Elegant boer goat ram and Macheng black goat male and female sheep, sets up breeding group respectively；Ram selection growth and development is fast, trunk produces
Meat performance height, the boer goat of the strong feature of disease resistance and Macheng black goat ram；Macheng black goat ewe selects prlificacy high, female
The goat that property is strong, milking capacity is good；
It (2) is female parent with Macheng black goat, boer goat is male parent, and hybridization obtains F1In generation, establishes F1Basis female flock of sheep；
(3) F1It is returned for basis female flock of sheep and Macheng black goat ram, obtains F2In generation, establishes F2Underlying group；
(4) F2Homogeneity apolegamy, which is carried out, for the sheep that underlying group reaches breeding requirement generates F3Generation；
The F not required up to breeding2Godmother flock of sheep are returned with Macheng black goat ram again, generate F3Generation；
F3Basic flock of sheep, ram contain 12 blood lineages to the zero of generation composition new lines from generation to generation, and ewe selects and remain 200, with ram
Family genetic connection sets up zero generation underlying group；
(5) the swarm robotic system breeding stage: use swarm robotic system breeding technique and multiple gene polymerization assistant breeding technology, 1.5 years
For a generation, it is carried out continuously the breeding of 4 generations.
Further, above-mentioned swarm robotic system breeding technique are as follows:
By strain development requirement, based on ram family genetic connection, 28 rams of 12 blood lineages are chosen, 567
Basic ewe amounts to 595 excellent individuals and forms zero generation underlying group；
In the 1st generation of group's Systematic Breeding, it is used to cultivate new varieties using multiple gene polymerization assisted Selection technology, it is selected
Select the male and female sheep individual for carrying polyembryony gene (FecB) heterozygous (B+) genotype；
It selects the interior individual being mutated there are a G/A of goat SKIP gene intron 2 and selects PRLR gene
The site 10611G/A, 11064C/T is as preponderant genotype；
Selection influences the MC1R gene of goat hair color, in the wean of each 3 monthly age of generation male and female sheep, respectively by related gene
Preponderant genotype as assisted Selection foundation, select and remain kind of sheep in conjunction with selection index and the compound breeding value of ewe litter size and draft
Apolegamy plan, this assisted Selection technology are applied successively to 2,3,4 each generations of new varieties group Systematic Breeding.
Further, above-mentioned swarm robotic system breeding technique and multiple gene polymerization assistant breeding technology include color inheritance choosing
It educates；
Color inheritance breeding is on the basis of hybridization synthesis and fixed traversed by, and hair color selection is latched numerous in 0 generation strain
Early period is educated based on test cross and descendant's investigation, based on stage is identified with pedigree；The frequency that pale brown miscellaneous hair occurs between generation is by 1 generation
The 20.32% of generation is down to the 1.56% of 4 generations, and genotype is substantially homozygous, and blackhead not holding any government official post's Coat Color as varietal characteristic is steady
Surely hereditary；
In color inheritance, white be to brown and black it is dominant, Macheng black goat and boer goat hair color mainly by with
Gene control on lower four locus:
1. wild type A:a determines that single black, aa are the black coat of non-wild type, the main basic hair color for controlling whole body；
2. the gene for controlling the hair color of incidence has tri- allele of T, t+ and t-, T expression white is for t+ and t-
Dominant, t+ expresses brown, expresses black for t-() it is dominant；
3. hickie seat S: having tri- allele of St, Sd and s, the main patch color for controlling whole body, St, Sd are complete to s
Complete dominant, gene s determines no hickie；
4. dilution gfactor seat D: there was only a pair of alleles on this seat, dominant D determines dark color, it is such as yellow, brown, black,
Recessive d desalinates hair color, this four genes are all on autosome, each other there is no chain, according to preliminary observation to different
Difference in cross combination, nest, stock ram effect are analyzed with ewe, propose its genetic mechanism it is assumed that and investigating pedigree note
Record carries out genetic analysis and comptibility test, determines blackhead not holding any government official post's coat color gene homozygote.
Further, the formula of the selection index of virgin wool color type individual are as follows:
P, q is the original hair color type determined by theoretical assumption and by the ratio for looking into virgin wool color type respectively；K, r generation respectively
The theoretical value of table family member number, family Central Plains hairiness color type number and virgin wool color type number of individuals；
According to the family member's number actually observed under various k values, number of elements should be had and have number of elements in fact by carrying out novel individual
Test of goodness of fit makees breeding stock to determine or negate theoretical assumption with test cross or the polynary test cross selection sub- ram of dominant homogeneous.
New lines blackhead not holding any government official post containing 12.5% boer goat blood lineage and 87.5% Macheng black goat blood lineage or contains 25%
Boer goat blood lineage and 75% Macheng black goat blood lineage, birth weight average weight ram is 2.5 kilograms or more, ewe is 2.3 kilograms
More than, 6 monthly age rams are 25 kilograms or more, ewe is 20 kilograms or more, and one full year of life ram is 45 kilograms or more, ewe is 36 kilograms
More than；65 kilograms of ram of adult or more, 50 kilograms of ewe of adult or more；Primiparity ewe lambing percentage 180%, through producing ewe lambing percentage
250%, 48% or more one full year of life dressing percentage, 150 grams of stage of fattening average daily gain；
Appearance traits: incidence coat black, body body coat white；Constitution is solid, and figure is big, and structure is well-balanced, and back waist is flat
Directly, rear quarters is plentiful, and four limbs are higher and sturdy, and whole body is at barrel-like structure.
Meat: pH1、pH24, Color Score and marble grain scoring 5.5 or more；Amino acid is abundant in mutton, and fatty acid contains
Amount has reached the World Health Organization and FAO's proposed standard；Stearic acid content is low, and smell of mutton is light；And cholesterol level
It is low.Muscular tissue structure, shear force value etc., meat quality are superior to local naked eyed test.
In the present invention breeding method of blackhead not holding any government official post mutton sheep new lines be using modern traditional breeding techniques, optimum linear without
Prediction BLUP and multiple gene polymerization assistant breeding technology combine partially.
Multiple gene polymerization assistant breeding technology includes: Selection utilization controls mutton sheep litter sizeFecBGene；Control growth
SKIP and the PRLR gene of speed, control goat hair color MC1R gene, and selective advantage genotype male and female sheep individual establishes breeding base
Plinth group (zero from generation to generation), from a generation to four it is each from generation to generation persistently use multiple gene polymerization assistant breeding technology from generation to generation, and combine the public affairs,
The hair color of ewe, litter size, daily gain carry out breeding.
Optimal linear unbiased prediction (best linear unbiased prediction, BLUP) is world today's range
Interior main breeding stock Genetic Evaluation method makes full use of the information of various aspects on the basis of eliminating various system environments errors,
A kind of accurate estimation of optimization is carried out to the breeding value of individual.
Multiple selection index method refer to all characters to be improved by its genetic force and economic effect be merged into one it is total
Then index selects this index as an individual event, the high person of index reserves seed for planting, and low person eliminates.
Multiple gene polymerization supplemental polymeric breeding is exactly to utilize this method of gene pyramiding, passes through conventional hybridization, baclccrossing techniques
Beneficial gene is aggregated to the same genome, contains of multiple target genes by Marker-assisted selection in segregating generation
Body therefrom filters out the single plant with excellent target character again, realizes the polymerization of beneficial gene.
The utility model has the advantages that
Breeding method of the invention is female parent with Macheng black goat, and boer goat is male parent, poly- using BLUP method, polygenes
It closes assistant breeding technology and combines conventional breeding methods, cultivate and take into account hair color, high reproductive rate, Meat Performance and energy high temperature resistant height
There is blackhead not holding any government official post's feature in wet meat goat new lines, stabilization, are significantly better than Macheng Black Hills in terms of growing with meat production
Sheep, reproductive performance, resistance to crude feed, it is disease-resistant in terms of be better than boer goat, reached the production performance of modern naked eyed test
It is horizontal.
Detailed description of the invention
Attached drawing is the flow chart of the embodiment of the present invention 1.
Indicate Macheng black goat；Indicate boer goat；Indicate black goat containing Macheng 75%, wave
That goat 25%；Black goat containing Macheng 87.5%, boer goat 12.5%；
The scene 1-: test cross and descendant's investigation carry out hair color selection；
Experimental determination:FecB- influence litter size；SKIP, PRLR-influence speed of growth；MC1R-influence hair color；
2- molecular marker assisted selection: selective advantage genotype individualsFecB,SKIP,PRLR,MC1R；
The following character of 3- field observation:
Hair color: blackhead not holding any government official post；
Weight: birth weight, weaning weight, one full year of life weight, adult weight (formulating selection index)；
Litter size: (formulating more relatives' information selection indexes)；
Meat: pH1, yellowish pink, percentage of water loss etc..
Specific embodiment
The invention will be further described below.
Embodiment one
Male parent selection: physique is big, the speed of growth is fast, it is straight, preceding to carry on the back waist first according to the production performance criterion for breeding of formulation
The outstanding ram that chest is open, male is full, appearance traits are good is just selected as breeding ram, then estimates stud ram breeding using BLUP method
Value and molecular marker assisted selection (MAS) finally identify achievement Comprehensive Assessment according to pedigree and descendant.Selection criteria: bohr mountain
Sheep is in dark-brown, rufous flower piece in head, neck, shoulder hair color, and back, abdomen, side portion hair are white；Forehead to lip end have one it is white
Color hair-videotape, nose are in claws of a hawk sample.Three-dimensional-structure is well-balanced, and physique is big, chest breadth is deep, shoulder breadth is thick, across back and straight, and rib cage opens good
Good, entire body body circle is thick and compact；Ram has angle, and grow up ram weight 70-85 kg, and one full year of life ram dressing percentage reaches 50%-
52%, 40% or more neat percentage.
Female parent selection: first according to the production performance criterion for breeding of formulation, reproductive performance is good, maternal instinct is strong, appearance traits are good
Outstanding ewe be just selected as ewe exposed to ram, recycle composite index law that ewe is instructed to choose seeds, finally identified according to itself and descendant
Performance rating grade.Selection criteria: Macheng black goat constitution is solid, structure is well-balanced.Whole body coat black, hair is short personal, there is light
Pool, neck are elongated, delicate and pretty.Neck shoulder is well combined, and shirtfront is flourishing, and rear quarters physically well develops, and back waist is straight, and four limbs rectify sturdy, hoof
Matter is solid, and breast is flourishing, effective nipple two, and there are two additional teats for some sheep, and tail is short to be upwarped.Adult ewe weight be respectively
40 kg.One full year of life sheep dressing percentage reaches 44%-46%, and 38% or more neat percentage is produced ewe and is averaged lambing percentage not less than 230%.
500 outstanding ewes are selected from the black goat of Macheng as female parent, and 30 outstanding rams are selected from boer goat
As male parent, the two is hybridized, and ram is all eliminated in given birth to F1 generation, is selected and is retained outstanding ewe 400, composition hybridization one
Godmother flock of sheep；
It is hair color, birth weight, weaning weight, six-month-old weight, week that the factor mainly considered when outstanding ewe is selected from F1 generation
Year weight, adult weight, physique is larger, and posture is delicate and pretty, birth weight 2.5kg or more, weaning weight 15kg or more, six-month-old weight 25kg with
On；
With F1 generation, ewe group is female parent, is returned using Macheng black goat as male parent, obtains F2 generation, wherein there is ram 25
Only, ewe 350.
Test cross and descendant's investigation are carried out, the sub- ram of blackhead not holding any government official post dominant homogeneous is selected to make kind of a sheep.It is selected and remain from F2 generation outstanding
The factor that ewe mainly considers is hair color, birth weight, weaning weight, six-month-old weight, and be with specific reference to standard: physique is larger, posture
Delicate and pretty, figure is like boer goat, birth weight 2.3kg or more, weaning weight 15kg or more, six-month-old weight 24kg or more；
With F2 for traversed by, F3 generation is obtained, F3 is for the Macheng black goat blood lineage containing 75%, 25% boer goat blood lineage；Or with
F2 godmother flock of sheep are female parent, are returned using Macheng black goat as male parent, obtain F3 generation, F2 is for the Macheng black goat containing 87.5%
Blood lineage, 12.5% boer goat blood lineage；Zero generation of new lines flock of sheep are formed, wherein have ram 20, ewe 300；
It is identical as the standard of outstanding ewe is selected and remain from F2 generation from the selection criteria for selecting and remain outstanding ewe in F3 generation, mainly examine
The factor of worry is hair color, birth weight, weaning weight, six-month-old weight, is with specific reference to standard: blackhead not holding any government official post, physique is larger, and posture is clear
Show, birth weight 2.3kg or more, weaning weight 15kg or more, six-month-old weight 24kg or more, only on shape and appearance, F3 godmother sheep
Closer to the physical characteristic of boer goat；
Selecting and remain the factor that outstanding ram mainly considers from F3 generation is appearance traits, birth weight, weaning weight, six-month-old weight,
Be with specific reference to standard: blackhead not holding any government official post, physique is larger, short and small, and short by capillary, glossy, figure is close with Boer goat, posture
Plentiful, testicualr development is good, and 2.5 kg or more of birth weight, 18 kg or more of weaning weight, six-month-old weigh 30 kg or more；Carry out test cross
It is investigated with descendant, the sub- ram of blackhead not holding any government official post dominant homogeneous is selected to make kind of a sheep.
The swarm robotic system breeding stage: by strain development requirement, based on ram family genetic connection, 12 blood lineages are chosen
28 rams, 567 basic ewes amount to 595 excellent individuals and form zero generation underlying groups.The 1st of group's Systematic Breeding the
A generation begins to using multiple gene polymerization assisted Selection technology for cultivating new varieties, selected carrying polyembryony gene
(FecB) the male and female sheep individual of heterozygous (B+) genotype；Selection influences SKIP and the PRLR gene of the speed of growth, selects goat
There are the positions 10611G/A, 11064C/T of the G/A individual being mutated and selection PRLR gene in SKIP gene intron 2
Point is used as preponderant genotype；Selection influences the MC1R gene of goat hair color, in the wean of each 3 monthly age of generation male and female sheep, respectively will
The preponderant genotype of related gene selects and remain kind as assisted Selection foundation, in conjunction with selection index and the compound breeding value of ewe litter size
Sheep and draft apolegamy plan, this assisted Selection technology be applied successively to new varieties group Systematic Breeding 2,3,4 it is each from generation to generation.
Each generation selects and remain standard: blackhead not holding any government official post, physique are larger, short and small, and short by capillary, glossy, ram posture is rich
Full, testicualr development is good, and ewe posture is delicate and pretty, and mammary development is good, how spherical in shape, ram birth weight 2.5kg or more, weaning weight
18kg or more, six-month-old weight 30kg or more, one full year of life 40kg or more, ewe birth weight 2.3kg or more, weaning weight 15kg or more, six
Monthly age 24kg or more, one full year of life 35kg or more.
BLUP method: under the pedigree data for ensuring that phenotypic information is true and reliable, collecting correctly complete situation, mixing is established
Model, estimated breeding value.It first has to filter out the fixed effect factor for influencing character, then designs possibility according to the actual situation
Mixed linear model, establish Mixed model mixed, then using mtdfremal software to the variance components of different models and
Its ratio is calculated, and is compared research again according to the size of variance proportion, so that suitable trait predictive model is filtered out,
Model calculates mutton sheep breeding value according to this again, finally filters out outstanding kind of sheep according to breeding value size.
Multiple selection index method: estimate various phenotypes (birth weight, weaning weight, 6 monthly age weights, the 12 monthly ages weight, adult of character
Weight, eye muscle area, litter size) parameter and genetic parameter；Determine economic weight；Estimate selection intensity；It formulates selection index, estimate
Meter selection effect；Calculate the index value of individual.
Breeding result
(1) appearance traits
Physique is larger, and constitution is solid, and body is long high slightly larger than body, is rectangle.With high-temp resisting high-humidity resisting, ecological environment is adapted to
Property it is good, neck is plentiful, moderate-length, and waist is straight for back, chest depth, and four limbs are rectified, and incidence is black, and body body portion is white, part
Sheep has a small amount of blackspot or the miscellaneous hair of black, and back waist is straight, and four limbs are healthy and strong, body body is plentiful, and structure is well-balanced, and joint is solid, and pastern is strong, hoof
Rectify, hard hooves.
(2) weight
Early growth and development and body weight increase situation are to reflect the important indicator of sheep viability and adaptability.It can from table 1
To find out, the birth weight of 4 generation male and female sheep is respectively 2.51 kilograms and 2.31 kilograms, than Macheng black goat birth weight 1.83 thousand
Gram and 1.75 kilograms greatly improve.
1 Different Month male and female sheep changes of weight of table
(3) carcass trait
From table 2 it can be seen that four Slaughter weights for being bred as new lines from generation to generation and carcass weight are compared with the black goat of Macheng, it is poor
It is different to reach extremely significant horizontal (P < 0.01)；Four dressing percentages for being bred as new lines from generation to generation and neat percentage are compared with the black goat of Macheng, difference
Up to the level of signifiance (P < 0.05), the results show that the main carcass trait of breeding offspring is superior to Macheng black goat.
Carcass trait (n=12) before and after 2 breeding of table
(4) Meat Quality
As can be seen from Table 3, except the pH of Macheng black goat24It is significantly higher than four to be bred as from generation to generation outside new lines (P < 0.05), respectively
Other main Meat Quality indexs are without significant difference (P > 0.05) between group.
Meat Quality (n=12) before and after 3 breeding of table
Amino acid composition and content.As can be seen from Table 4, total amino acid content, essential amino acid and Fresh ear field contain
Amount size order is four generations to be bred as new lines > Macheng black goat, although 3 indicator differences are not significant between each group, new product
It is that the trend that goat meat makes moderate progress is obvious；In addition, each group Glutamic Acid, aspartic acid, leucine and lysine content
Higher, wherein content of glutamic acid highest, glutamic acid have the special work for forming meat delicate flavour and buffering the disagreeable tastes such as salty, sour
With therefore, from amino acid composition, new lines not only have higher protein " biological value ", and strongly fragrant for the perfume of meat flavour
Provide material base.
The composition and content (n=12, %) of amino acid before and after 4 breeding of table
Fatty acid composition.Fatty acid determination in muscle the results show that main fatty acid myristic acid (C14:0) in muscle,
Palmitinic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), linolenic acid
(C18:3) accumulative composition accounts for total fatty acids 94.02%-94.35%.2 groups of mutton sheep fatty acid compositions, which are compared, following features: 3 groups
The highest fatty acid of content is all oleic acid (C18:1), palmitinic acid (C16:0) and stearic acid (C18:0), and linoleic acid (C18:2) is
Most important polyunsaturated fatty acid (PUFA), followed by arachidonic acid (C20:4) and linolenic acid (C18:3).Primary fat
In acid composition, four are bred as the composition and Macheng black goat group significant difference (P < 0.05) of new lines linoleic acid (C18:2) from generation to generation,
Other fatty acid group Chengdu are not significant (P > 0.05).
Analysis of Fatty Acids Composition (n=12, %) before and after 5 breeding of table
(5) research of color inheritance
On the basis of hybridization synthesis and fixed traversed by, hair color is strictly eliminated, hair color selection is latched in 0 generation strain breeds
Early period based on test cross and descendant's investigation, based on stage is identified with pedigree, solves the difficulty that hair color Phenotypic Selection is made slow progress
Topic, improves hair color efficiency of selection.The frequency that pale brown miscellaneous hair occurs between generation was down to for 4 generations by the 20.32% of 1 generation
1.56%, genotype is substantially homozygous, and the blackhead not holding any government official post Coat Color as varietal characteristic is steadily hereditary.
Strictly superseded hair color, hair color selection are latched breeding early period based on test cross and descendant's investigation in strain, stage with
Based on pedigree identification.The heredity of boer goat color of head and neck belongs to autosomal recessive inheritance.In color inheritance, white
Be to brown and black it is dominant, according to analysis Macheng black goat and boer goat hair color mainly by the base on following four locus
Because of control: 1. wild type A:a determines that single black, aa are the black coat of non-wild type, the main basic hair color for controlling whole body；②
The gene for controlling the hair color of incidence has tri- allele of T, t+ and t-, and T expression white, is dominant, t+ table for t+ and t-
Up to brown, black is expressed for t-() it is dominant；3. hickie seat S: having tri- allele of St, Sd and s, mainly control whole body
Patch color, to s complete dominance, gene s determines no hickie by St, Sd；4. dilution gfactor seat D: on this seat only
A pair of alleles, dominant D determine dark color, such as yellow, brown, black, and recessive d desalinates hair color, this four genes are all dyed often
On body, each other there is no chain, according to difference in preliminary observation to Different Cross Combinations, nest, stock ram effect, with match ewe into
Row analysis, propose its genetic mechanism it is assumed that and investigate pedigree record, carry out genetic analysis and comptibility test, determine blackhead
Not holding any government official post's coat color gene homozygote.
It in analysis, determines when having occurred by the brood lamb for looking into virgin wool color type, different each of virgin wool color type number
The probability and number of members of kind family are the formula of the theoretical value of virgin wool color type individual in the family of definite value are as follows:
P, q is the original hair color type determined by theoretical assumption and by the ratio for looking into virgin wool color type respectively；K, r, difference
Represent the theoretical value of family member's number, family Central Plains hairiness color type number and virgin wool color type number of individuals.
According to the family member's number actually observed under various k values, number of elements should be had and have number of elements in fact by carrying out novel individual
Test of goodness of fit selects (single seat position or more seats) dominant homogeneous to determine or negate theoretical assumption with test cross or polynary test cross
Sub- ram makees breeding stock, is effective fixing means.
The color inheritance of goat is controlled by multipair gene, and white is in dominant inheritance to black, and wave is presented in the hair color of a miscellaneous generation
The feature of your sheep, hair color segregation phenomenon will occur in traversed by offspring, in Breeding Process, Yao Jiaqiang hair color purity and black depth
Selection.
(6) genetic parameter estimation of reproductive trait
Using multiple traits derivative free restricted maximum likelihood (multiple traits derivative free
Restricted maximum likelihood, MTDFREML) to totally 2137 new lines (kind) goats of 2008-2015
Growth Traits carry out genetic parameter estimation, the results showed that, the genetic force difference that weight, body height, Body steep length, bust and pipe enclose
It is 0.35,0.38,0.32,0.36 and 0.30, it is strong that the Genetic correlation coefficient between this 5 Growth Traits is all higher
It is positively correlated.The genetic force estimated result of ewe reproductive trait: birth weight, weaning weight, litter size, lamb number living, weight of weaning litter and disconnected
The genetic force of milk lamb number is respectively 0.16,0.18,0.11,0.16,0.20,0.24.Birth weight, weaning weight respectively with other
Genetic correlation between each reproductive trait related to phenotype is mostly weak positive correlation or negative correlation, litter size, lamb number living, wean nest
Genetic correlation between weight and wean number of individuals is all to be positively correlated by force.
(7) analysis of genetic polymorphisms of caprine growth related gene SKIP and PRLR
It was found that there are a G/A to be mutated in goat SKIP gene intron 2, PCR-BgII-RFLP digestion point is established
Type method finds that SKIP gene polymorphic and certain growth traits of goat are significant related, such as 3-6 monthly age daily gain, 3 monthly age chests
It encloses, 12 monthly age pipes enclose, 12 monthly age weight, 18 monthly age body height etc.；The site 10611G/A, 11064C/T of PRLR gene and goat are raw
Long character is in significant correlation, 10611G/A and 11064C/T combination gene type GGCC proportion is maximum, is 41.7%, GGTT base
It is unfavorable genotype combination because type individual shows generally on many growth performances；The reproductive ability in the site 10611G/A and goat
Shape is related, and GA genotype has bigger litter weight and birth weight compared with GG genotype offspring, shows GA genotype relative to GG base
Because type has more excellent offspring.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (2)
1. a kind of breeding method of blackhead not holding any government official post mutton sheep new lines, which is characterized in that
The following steps are included:
(1) boer goat and Macheng black goat are identified and is assessed using conventional selection combination BLUP method, selected outstanding
Boer goat ram and Macheng black goat male and female sheep, set up breeding group respectively；Ram selection growth and development is fast, trunk produces meat
It can the high, boer goat of the strong feature of disease resistance and Macheng black goat ram；Macheng black goat ewe selects prlificacy height, maternal instinct
By force, the good goat of milking capacity；
It (2) is female parent with Macheng black goat, boer goat is male parent, and hybridization obtains F1In generation, establishes F1Basis female flock of sheep；
(3) F1It is returned for basis female flock of sheep and Macheng black goat ram, obtains F2In generation, establishes F2Underlying group；
(4) F2Homogeneity apolegamy, which is carried out, for the sheep that underlying group reaches breeding requirement generates F3Generation；
The F not required up to breeding2Godmother flock of sheep are returned with Macheng black goat ram again, generate F3Generation；
F3Basic flock of sheep, ram contain 12 blood lineages to the zero of generation composition new lines from generation to generation, and ewe selects and remain 200, with ram family blood
Edge relationship sets up zero generation underlying group；
(5) the swarm robotic system breeding stage: using swarm robotic system breeding and multiple gene polymerization assistant breeding, and 1.5 years are a generation, even
The continuous breeding for carrying out 4 generations；
The swarm robotic system breeding are as follows:
By strain development requirement, based on ram family genetic connection, 28 rams of 12 blood lineages, 567 bases are chosen
Ewe amounts to 595 excellent individuals and forms zero generation underlying group；
In the 1st generation of swarm robotic system breeding, it is used to cultivate new varieties using multiple gene polymerization assisted Selection technology, it is selected
Carry the male and female sheep individual of polyembryony gene FecB heterozygous B+ genotype；
It selects the interior individual being mutated there are a G/A of goat SKIP gene intron 2 and selects the 10611G/ of PRLR gene
A, the site 11064C/T is as preponderant genotype；
Selection influences the MC1R gene of goat hair color, in the wean of each 3 monthly age of generation male and female sheep, respectively by the excellent of related gene
Gesture genotype selects and remain kind of sheep in conjunction with selection index and the compound breeding value of ewe litter size and drafts apolegamy as assisted Selection foundation
Plan, this assisted Selection technology are applied successively to 2,3,4 each generations of new varieties group Systematic Breeding.
The swarm robotic system breeding and multiple gene polymerization assistant breeding include color inheritance breeding；
The color inheritance breeding is on the basis of hybridization synthesis and fixed traversed by, and hair color selection is latched numerous in 0 generation strain
Early period is educated based on test cross and descendant's investigation, based on stage is identified with pedigree；The frequency that pale brown miscellaneous hair occurs between generation is by 1 generation
The 20.32% of generation is down to the 1.56% of 4 generations, and genotype is substantially homozygous, and blackhead not holding any government official post's Coat Color as varietal characteristic is steady
Surely hereditary；
In color inheritance, white be to brown and black it is dominant, Macheng black goat and boer goat hair color are mainly by following four
Gene control on a locus:
1. wild type A:a determines that single black, aa are the black coat of non-wild type, the main basic hair color for controlling whole body；
2. the gene for controlling the hair color of incidence has tri- allele of T, t+ and t-, T expression white, is aobvious for t+ and t-
Property, t+ expresses brown, is dominant for t- expression black；
3. hickie seat S: having tri- allele of St, Sd and s, the main patch color for controlling whole body, St, Sd are completely aobvious to s
Property, gene s determines no hickie；
4. dilution gfactor seat D: there was only a pair of alleles on this seat, dominant D determines dark, such as yellow, brown, black, recessive d
Desalinate hair color, this four genes are all on autosome, each other there is no chain, according to preliminary observation to different hybridization groups
Close, difference in nest, stock ram effect, with match ewe and analyzed, propose its genetic mechanism it is assumed that and investigate pedigree record, into
Row genetic analysis and comptibility test determine blackhead not holding any government official post's coat color gene homozygote.
2. breeding method as described in claim 1, which is characterized in that
The formula of the selection index of virgin wool color type individual are as follows:
P, q is the original hair color type determined by theoretical assumption and by the ratio for looking into virgin wool color type respectively；K, r respectively represents house
The theoretical value of set member's number, family Central Plains hairiness color type number and virgin wool color type number of individuals；
According to the family member's number actually observed under various k values, number of elements should be had and have being suitble to for number of elements in fact by carrying out novel individual
Property measurement, with determine or negative theoretical assumption, with test cross or polynary test cross selection the sub- ram of dominant homogeneous make breeding stock.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710618155.5A CN107347796B (en) | 2017-07-26 | 2017-07-26 | A kind of breeding method of blackhead not holding any government official post mutton sheep new lines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710618155.5A CN107347796B (en) | 2017-07-26 | 2017-07-26 | A kind of breeding method of blackhead not holding any government official post mutton sheep new lines |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107347796A CN107347796A (en) | 2017-11-17 |
| CN107347796B true CN107347796B (en) | 2019-10-01 |
Family
ID=60284946
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710618155.5A Expired - Fee Related CN107347796B (en) | 2017-07-26 | 2017-07-26 | A kind of breeding method of blackhead not holding any government official post mutton sheep new lines |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107347796B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109744195B (en) * | 2018-12-28 | 2020-12-15 | 广州影子科技有限公司 | Accurate matching method using parental genome information in animal breeding |
| CN110214744A (en) * | 2019-05-28 | 2019-09-10 | 临沂市农业科学院 | A kind of Yimeng black goat purification and rejuvenation method |
| CN114794010B (en) * | 2022-03-08 | 2023-04-07 | 安徽黄氏番鸭食品有限公司 | Method for breeding lean meat type Muscovy duck strain based on molecular marker |
| WO2024060111A1 (en) * | 2022-09-22 | 2024-03-28 | 中国农业大学 | Novel method for improving stress resistance of sheep in childhood |
-
2017
- 2017-07-26 CN CN201710618155.5A patent/CN107347796B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN107347796A (en) | 2017-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107347796B (en) | A kind of breeding method of blackhead not holding any government official post mutton sheep new lines | |
| CN100369544C (en) | Method for raising new strain of lean meat type Huai pigs | |
| CN101755707B (en) | Method for breeding new species of lean meat type pigs | |
| CN1871903B (en) | Method for breed porker by using Changbai mountain wild boar as male parent and Songliao black pig as female parent | |
| CN104285884B (en) | A kind of breeding method of Jin Fen white pig new varieties | |
| CN110367197B (en) | Hybridization matching combination research method of meat pigeons | |
| CN107821314A (en) | A kind of method for cultivating high-quality specialized strain using pig is hidden | |
| CN106212380B (en) | A kind of Small-fat-tail sheep selects excellent method of purification | |
| CN106614356B (en) | Method for breeding Sumei black pigs | |
| CN108419756A (en) | A kind of Ba Huai pig breeding methods | |
| Krider et al. | Effectiveness of selecting for rapid and for slow growth rate in Hampshire swine | |
| CN104719255A (en) | Cultivation method for new black pig varieties | |
| CN103734079B (en) | The mating system selective breeding method of Market pigs | |
| CN109511608A (en) | A kind of breeding method of the meat new lines of high mountain Merino | |
| CN108935304A (en) | A kind of cross breeding method of the black pig of Yangze river and Huai river | |
| CN109105338A (en) | A kind of producing method for seed of red plumage white shell egg chicken breed system | |
| CN108184760A (en) | A kind of breeding method of black meat rabbit new lines | |
| KR101757619B1 (en) | Preparing method of synthetic pig by a cross between Duroc and Korean native pig, and synthetic pig using the same | |
| CN107494415B (en) | Method for breeding new Guangdong yellow chickens | |
| Nassambaev et al. | Pure breeding of the Kazakh white-headed cattle by lines as the main method of improving the hereditary qualities | |
| CN109122579B (en) | Method for improving self-identification sexing accuracy of fast and slow feathers of big-bone chicken | |
| CN109042494A (en) | A kind of seed choosing method of black pig new varieties | |
| CN104886003A (en) | Hybridization method for improving porcine intramuscular fat content | |
| Stancheva et al. | Genetic structure of the sheep from the Shumen inbreeding type of north-east Bulgarian merino sheep. | |
| CN108739665A (en) | A kind of selection of Berkshire |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191001 |