CN107347646A - A kind of purple perilla special culture media - Google Patents
A kind of purple perilla special culture media Download PDFInfo
- Publication number
- CN107347646A CN107347646A CN201710665923.2A CN201710665923A CN107347646A CN 107347646 A CN107347646 A CN 107347646A CN 201710665923 A CN201710665923 A CN 201710665923A CN 107347646 A CN107347646 A CN 107347646A
- Authority
- CN
- China
- Prior art keywords
- purple perilla
- methyl
- culture media
- growth
- triacontanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a kind of purple perilla special culture media, including following components：1/4 1/2MS culture mediums, 0.2 0.4mg/L methyl α-naphthyl acetates, 0.7 1mg/L brassinosteroids, 0.8 1.2mg/L triacontanols, 0.02 0.05mg/L daminozides, 3 5g/L white granulated sugars, 8 10g/L agar.The present invention with the addition of brassinosteroid in traditional plant culture for purple perilla, triacontanol and daminozide, both triacontanol and methyl α-naphthyl acetate cooperate with the growth that can effectively facilitate purple perilla root growth and cauline leaf, and a small amount of daminozide can cooperate with the effect of each component, adjust the nutrient distribution in purple perilla incubation, so that purple perilla root growth is more solid, it is easy to transplant survival, in addition, brassinosteroid, triacontanol and daminozide three have different degrees of disease resistance, the growth of germ can be suppressed, improve the survival rate of purple perilla, significantly reduce purple perilla melting brown rate.
Description
Technical field
The present invention relates to plant tissue culture technique, and in particular to a kind of purple perilla special culture media.
Background technology
The red Soviet Union of purple perilla alias, red Soviet Union, black Soviet Union, purple Soviet Union, wrinkle purple perilla etc..Purple perilla is the band of Labiatae purple perilla platymiscium purple perilla
Leaf spray.It is used as medicine in fact with stem, leaf and son.It is i.e. pharmaceutically acceptable that purple perilla belongs to comprehensive crossover commodity, is clinical common medicine, and can is eaten.
Purple perilla sprout has special fragranced, is China's the South and the North wide geographic area shoot vegetable in very great demand, and sell to Chinese Fragrant
The feature breed in the area such as port, TaiWan, China.Purple perilla sprout is rich in abundant vitamin C, potassium, iron etc., also containing abundant insatiable hunger
And aliphatic acid, body immunity can be improved, has and relaxes the anaphylactoid effects such as allergic dermatitis, pollinosis, infected by influenza
There is certain inhibitory action.
Existing purple perilla culture medium generally uses MS culture mediums, and adds the common plant growth substance such as indolebutyric acid, but
It is that rooting efficiency and rooting rate are still undesirable, it would therefore be desirable to which it is good to study a kind of rooting efficiency, can effectively ensure that rooting rate
Purple perilla special culture media.
The content of the invention
Promote purple perilla root growth it is an object of the invention to provide one kind, improve purple perilla transplanting survival rate, while can
Suppress pathogen growth, reduce the purple perilla special culture media of melting brown rate.
The technical solution for realizing the object of the invention is：
A kind of purple perilla special culture media, including following components：1/4-1/2MS culture mediums, 0.2-0.4mg/L methyl α-naphthyl acetates,
0.7-1mg/L brassinosteroids, 0.8-1.2mg/L triacontanols, 0.02-0.05mg/L daminozides, 3-5g/L white granulated sugars, 8-
10g/L agar, the pH value of culture medium is 6-7.
In further embodiment, culture medium preferably includes following components：1/2MS culture mediums, 0.4mg/L methyl α-naphthyl acetates,
0.8mg/L brassinosteroids, 0.8mg/L triacontanols, 0.02mg/L daminozides, 4g/L white granulated sugars, 10g/L agar.
Compared with prior art, its remarkable advantage is the present invention：
The present invention in traditional plant culture for purple perilla with the addition of can promote purple perilla grow brassinosteroid,
Both triacontanol and daminozide, triacontanol and methyl α-naphthyl acetate act synergistically, and can effectively facilitate purple perilla root growth, rape element
Lactone can promote the growth of cauline leaf, and a small amount of daminozide can cooperate with the effect of each component, adjust in purple perilla incubation
Nutrient distribution so that purple perilla root growth is more solid, is easy to transplant survival, in addition, brassinosteroid, triacontanol and butyryl
Hydrazine three has different degrees of disease resistance, can suppress the growth of germ, carries out effectively sterilization regulation, improves surviving for purple perilla
Rate, and then prevent germ from encroaching on and significantly reduce purple perilla melting brown rate.
Embodiment
The present invention is described in further detail with reference to specific embodiment.
Embodiment 1
A kind of purple perilla special culture media, including following components：1/4MS culture mediums, 0.2mg/L methyl α-naphthyl acetates, 0.7mg/L oil
Rape lactone, 1.2mg/L triacontanols, 0.05mg/L daminozides, 3g/L white granulated sugars, 8g/L agar, the pH value of culture medium is 6-
7。
Embodiment 2
A kind of purple perilla special culture media, including following components：1/2MS culture mediums, 0.3mg/L methyl α-naphthyl acetates, 1mg/L rapes
Plain lactone, 1mg/L triacontanols, 0.04mg/L daminozides, 5g/L white granulated sugars, 9g/L agar, the pH value of culture medium is 6-7.
Embodiment 3
A kind of purple perilla special culture media, including following components：1/2MS culture mediums, 0.4mg/L methyl α-naphthyl acetates, 0.8mg/L oil
Rape lactone, 0.8mg/L triacontanols, 0.02mg/L daminozides, 4g/L white granulated sugars, 10g/L agar, the pH value of culture medium are
6-7。
Three groups of control groups are prepared to test as a comparison：
Control group 1
A kind of purple perilla special culture media, including following components：1/2MS culture mediums, 0.4mg/L methyl α-naphthyl acetates, 0.8mg/L tri-
Ten alkanols, 0.02mg/L daminozides, 4g/L white granulated sugars, 10g/L agar, the pH value of culture medium is 6-7.
Control group 2
A kind of purple perilla special culture media, including following components：1/2MS culture mediums, 0.4mg/L methyl α-naphthyl acetates, 0.8mg/L oil
Rape lactone, 0.02mg/L daminozides, 4g/L white granulated sugars, 10g/L agar, the pH values of culture medium are 6-7.
Control group 3
A kind of purple perilla special culture media, including following components：1/2MS culture mediums, 0.4mg/L methyl α-naphthyl acetates, 0.8mg/L oil
Rape lactone, 0.8mg/L triacontanols, 4g/L white granulated sugars, 10g/L agar, the pH values of culture medium are 6-7.
Control group 4
A kind of purple perilla special culture media, including following components：1/2MS culture mediums, 0.4mg/L methyl α-naphthyl acetates, 4g/L white sands
Sugar, 10g/L agar, the pH value of culture medium is 6-7.
The purple perilla nutrient media components of table 1 and its content
The stem with bud purple perilla of clip 3-4cm length goes to above-described embodiment and the culture medium of control group is in intensity of illumination
2000lux, temperature are cultivated under conditions of being 25 ± 1 DEG C, and the data obtained see the table below：
Claims (2)
1. a kind of purple perilla special culture media, it is characterised in that including following components：1/4-1/2MS culture mediums, 0.2-0.4mg/L
Methyl α-naphthyl acetate, 0.7-1mg/L brassinosteroids, 0.8-1.2mg/L triacontanols, 0.02-0.05mg/L daminozides, 3-5g/L white sands
Sugar, 8-10g/L agar, the pH value of culture medium is 6-7.
2. a kind of purple perilla special culture media according to claim 1, it is characterised in that including following components：1/2MS is cultivated
Base, 0.4mg/L methyl α-naphthyl acetates, 0.8mg/L brassinosteroids, 0.8mg/L triacontanols, 0.02mg/L daminozides, 4g/L white granulated sugars,
10g/L agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710665923.2A CN107347646A (en) | 2017-08-04 | 2017-08-04 | A kind of purple perilla special culture media |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710665923.2A CN107347646A (en) | 2017-08-04 | 2017-08-04 | A kind of purple perilla special culture media |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107347646A true CN107347646A (en) | 2017-11-17 |
Family
ID=60288281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710665923.2A Pending CN107347646A (en) | 2017-08-04 | 2017-08-04 | A kind of purple perilla special culture media |
Country Status (1)
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| CN (1) | CN107347646A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109156351A (en) * | 2018-09-29 | 2019-01-08 | 江苏农林职业技术学院 | A kind of culture medium improving tissue culture plant inductivity and preparation method and application |
| CN112273227A (en) * | 2020-10-15 | 2021-01-29 | 永州职业技术学院 | Culture medium for improving survival rate of explants and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953337A (en) * | 2010-09-21 | 2011-01-26 | 中国科学院植物研究所 | Plant growth regulator composition and application thereof |
| CN104429859A (en) * | 2014-12-13 | 2015-03-25 | 天津宜耀科技有限公司 | Soilless cultivation method of Perilla |
| CN104886136A (en) * | 2015-05-27 | 2015-09-09 | 广西苷亮健生物科技有限公司 | Mixed rooting agent suitable for various plants |
-
2017
- 2017-08-04 CN CN201710665923.2A patent/CN107347646A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953337A (en) * | 2010-09-21 | 2011-01-26 | 中国科学院植物研究所 | Plant growth regulator composition and application thereof |
| CN104429859A (en) * | 2014-12-13 | 2015-03-25 | 天津宜耀科技有限公司 | Soilless cultivation method of Perilla |
| CN104886136A (en) * | 2015-05-27 | 2015-09-09 | 广西苷亮健生物科技有限公司 | Mixed rooting agent suitable for various plants |
Non-Patent Citations (5)
| Title |
|---|
| 余素芹: "《生物技术概要》", 31 December 1999, 贵州科技出版社 * |
| 唐湘如: "《作物栽培学》", 31 August 2014, 广东高等教育出版社 * |
| 纪明山等: "《新编农药使用技术》", 31 August 2010, 辽宁大学出版社 * |
| 谭文澄等: "生长素、细胞***素和三十烷醇对米兰空中压条生根的影响", 《广西植物》 * |
| 黄美娟等: "紫苏的组织培养及快繁技术研究", 《北方园艺》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109156351A (en) * | 2018-09-29 | 2019-01-08 | 江苏农林职业技术学院 | A kind of culture medium improving tissue culture plant inductivity and preparation method and application |
| CN112273227A (en) * | 2020-10-15 | 2021-01-29 | 永州职业技术学院 | Culture medium for improving survival rate of explants and preparation method and application thereof |
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Application publication date: 20171117 |
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