CN107340171A - A kind of separate type system for pretreating sample - Google Patents
A kind of separate type system for pretreating sample Download PDFInfo
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- CN107340171A CN107340171A CN201710689588.XA CN201710689588A CN107340171A CN 107340171 A CN107340171 A CN 107340171A CN 201710689588 A CN201710689588 A CN 201710689588A CN 107340171 A CN107340171 A CN 107340171A
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- 239000000523 sample Substances 0.000 claims description 232
- 230000007246 mechanism Effects 0.000 claims description 81
- 239000007788 liquid Substances 0.000 claims description 71
- 238000006243 chemical reaction Methods 0.000 claims description 50
- 238000004140 cleaning Methods 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 17
- 239000012530 fluid Substances 0.000 claims description 16
- 238000005119 centrifugation Methods 0.000 claims description 15
- 239000006166 lysate Substances 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 9
- 238000011534 incubation Methods 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000008280 blood Substances 0.000 abstract description 22
- 210000004369 blood Anatomy 0.000 abstract description 22
- 238000002360 preparation method Methods 0.000 abstract description 11
- 238000004458 analytical method Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 19
- 230000008569 process Effects 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 9
- 108010006464 Hemolysin Proteins Proteins 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 239000003228 hemolysin Substances 0.000 description 8
- 239000002699 waste material Substances 0.000 description 8
- 230000008450 motivation Effects 0.000 description 7
- 230000035939 shock Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 238000005063 solubilization Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000004895 subcellular structure Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention discloses a kind of separate type system for pretreating sample that can perform sample preparation before machine analysis in a manner of full automation on flow cytometer, the shortcomings that system can be good at overcoming artificial treatment to bring, the cell preparation work of blood sample before machine analysis on flow cytometer is completed, artificial and can be largely saved and improve blood sample treatment effect.
Description
Technical field
The invention belongs to cytologic technology field, and in particular to a kind of separate type system for pretreating sample.
Background technology
Flow cytometry (Flow Cytometry, FCM) is that one kind can quickly be surveyed to cell or subcellular structure
The new analytical technology and sorting technology of amount.It is characterized in：1. measuring speed is fast, can most be measured soon at 1 second in kind tens thousand of thin
Born of the same parents；2. measuring multiple parameters can be carried out, the measuring multiple parameters about Wuli-Shili-Renli system approach can be done to same cell, and have
Obvious statistical significance；3. being a comprehensive high-tech approach, it combines laser technology, computer technology, fluid
Mechanics, cytochemistry, image technique etc. are from multi-field knowledge and achievement；It is accurate point again 4. being both cell analysis technology
Selecting technology.It is that cell sample prepares quality to influence one of key factor of flow cytometry success or failure.It is thin to be presently used for streaming
The cell sample of born of the same parents' instrument detection, it is most of at home to examine section office to rely primarily on manual preparation.
But had the following disadvantages using cell sample is prepared by hand：
1st, the sample added by hand is relatively more, wastes sample resource.
2nd, the antibody added by hand is more or less, and antibody is expensive heavy, waste or deficiency be present, influences machine on final gained sample
Testing result.
3rd, manual preparation process easily malfunctions.
4th, manual preparation time length, efficiency are low.
5th, prepare by hand and rely on operating experience, nobody is available for domestic many section office, and there are flow cytometer in some section office, but not
Sample can be prepared, to lean on service provider to provide sample preparation service.
6th, manual operations can not realize great amount of samples preparation work.
7th, the cell concentration uniformity of the cell sample prepared by hand is poor.
The content of the invention
It is an object of the invention to overcome above-mentioned the deficiencies in the prior art, there is provided one kind can divide machine on flow cytometer
The separate type system for pretreating sample of sample preparation is performed before analysis in a manner of full automation.
The purpose of the present invention is achieved through the following technical solutions：
A kind of separate type system for pretreating sample, including：
Sample disk module, including sample disk and the first rotary drive mechanism for driving the sample disc spins, it is described
Sample disk is circumferentially equidistantly arranged with multiple sample tube seats；
Antibody disk module, including antibody disk and the second rotary drive mechanism for driving the antibody disc spins, it is described
Antibody disk is circumferentially equidistantly arranged with multiple antibody tube seats；
First liquid relief arm module, including the first probe, the first mechanical arm for fixing first probe, for driving
The first drive mechanism and the second driving machine for driving the first mechanical arm to move up and down of the first mechanical arm rotation
Structure, first probe are used to draw and fill antibody and sample；
Second liquid relief arm module, including the second probe, the second mechanical arm for fixing second probe, for driving
The 3rd drive mechanism and the 4th driving machine for driving the second mechanical arm to move up and down of the second mechanical arm rotation
Structure, second probe are used to fill lysate and buffer solution；
Disk module, including centrifugal pan and the 3rd rotary drive mechanism for driving the centrifugation disc spins are centrifuged, it is described
Centrifugal pan is circumferentially equidistantly arranged with multiple reaction pipe holders, and at least one reaction is provided with each reaction pipe holder
Tube seat, the centrifugal pan be used for realize the sample, the antibody, the lysate and the buffer solution incubation, mix and
Centrifugation, the 3rd rotary drive mechanism are used to drive the centrifugal pan realization to rotate clockwise or counterclockwise and be accurately positioned；
3rd liquid relief arm module, including the 3rd probe, the three-mechanical arm for fixing the 3rd probe, for driving
The 5th drive mechanism and the 6th driving machine for driving the three-mechanical arm to move up and down of the three-mechanical arm rotation
Structure, the 3rd probe are used to aspirate the supernatant after centrifugation.
Further, the separate type system for pretreating sample also includes cleaning module, and the cleaning module includes cleaning
Pump, syringe pump, magnetic valve, fluid path pipeline, service sink and cleaning fluid bucket, first probe, second probe and described
Three probes are connected with the syringe pump respectively, and the syringe pump is connected by the magnetic valve with the cleaning pump, the cleaning
Pump intake is connected with the cleaning fluid bucket, and the cleaning pump discharge is connected by the fluid path pipeline with the service sink.
Further, the service sink includes the first service sink, the second service sink and the 3rd service sink, first cleaning
Pond is arranged on below first probe, and second service sink is arranged on below second probe, the 3rd service sink
It is arranged on below the 3rd probe.
Further, the centrifugal pan makes the sample by the vibrations of itself and the liquid impulse force of the filling lysate
Originally, the antibody and the lysate mix.
Further, the 3rd rotary drive mechanism side is provided with for reducing the slow of the centrifugal pan vibration amplitude
Rush bearing.
Further, the first mechanical arm is driven with first drive mechanism and described second successively by first rotating shaft
Motivation structure connects, in the distance between the first rotating shaft and the sample disk center and the first rotating shaft and the antibody disk
The distance between heart is equal.
Further, the first liquid level sensor is provided with the first mechanical arm, first liquid level sensor is used for
The liquid level of antibody in antibody pipe described in automatic detection, the second liquid level sensor is provided with the three-mechanical arm, it is described
Second liquid level sensor is used for the liquid level of supernatant in reaction tube described in automatic detection.
Further, the outside of the antibody disk is provided with the first temperature control cavity, and first temperature control cavity is used to make described resist
The temperature of body disc maintains 2~8 DEG C；The outside of the centrifugal pan is provided with the second temperature control cavity, and second temperature control cavity is used to make
The reaction temperature of the centrifugal pan maintains 37 DEG C.
Further, sample tube is placed with the correspondence position of the sample tube seat, the sample tube is described for containing
Sample；Antibody pipe is placed with the correspondence position of the antibody tube seat, the antibody pipe is used to contain the antibody；The reaction
Reaction tube is placed with the correspondence position of tube seat, the sample, the antibody and the lysate are loaded with the reaction tube
The reaction solution of formation.
Further, the separate type system for pretreating sample also includes operational control module, the operational control module
For control the sample disk module, the antibody disk module, the first liquid relief arm module, the second liquid relief arm module,
The action of the 3rd liquid relief arm module and the centrifugation disk module.
Optionally, the separate type system for pretreating sample also includes scan sample module, and the scan sample module is set
It is placed on the outside of the sample tube, and is connected with the operational control module, can be put into by scanning on the outside of the sample tube two
Code is tieed up, the operational control module is fed back to, the experiment flow of the sample tube is controlled by the operational control module.
The present invention has the advantages that：
The invention provides a kind of separate type system for pretreating sample, the system can be good at overcoming artificial treatment to bring
The shortcomings that, the cell preparation work of blood sample before machine analysis on flow cytometer is completed, artificial and can be largely saved and carry
High blood sample treatment effect.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art and advantage, below will be to implementing
The required accompanying drawing used is briefly described in example or description of the prior art, it should be apparent that, drawings in the following description are only
Only it is some embodiments of the present invention, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the dimensional structure diagram of separate type system for pretreating sample provided by the invention；
Fig. 2 is the internal structure schematic diagram of separate type system for pretreating sample provided by the invention；
Fig. 3 is the front view of separate type system for pretreating sample provided by the invention；
Fig. 4 is the top view of separate type system for pretreating sample provided by the invention；
Fig. 5 is the dimensional structure diagram of sample disk module provided by the invention；
Fig. 6 is the dimensional structure diagram of antibody disk module provided by the invention；
Fig. 7 is the dimensional structure diagram of centrifugation disk module provided by the invention；
In figure：1- sample disks, the rotary drive mechanisms of 2- first, 3- sample tube seats, 4- antibody disks, the rotary driving machines of 5- second
Structure, 6- antibody tube seats, the probes of 7- first, 8- first mechanical arms, the drive mechanisms of 9- first, the drive mechanisms of 10- second, 11- second
Probe, 12- second mechanical arms, the drive mechanisms of 13- the 3rd, 14- centrifugal pans, the rotary drive mechanisms of 15- the 3rd, 16- reaction tube branch
Frame, 17- reaction tube seats, the probes of 18- the 3rd, 19- three-mechanical arms, the drive mechanisms of 20- the 5th, the drive mechanisms of 21- the 6th, 22-
First service sink, the service sinks of 23- second, the service sinks of 24- the 3rd, 25- cushioning support-saddles, 26- first rotating shafts, the rotating shafts of 27- second,
The rotating shafts of 28- the 3rd, the temperature control cavities of 29- first, the temperature control cavities of 30- second, 31- touch-screens, 32- instrument hosts.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art obtained on the premise of creative work is not made it is all its
His embodiment, belongs to the scope of protection of the invention.
In the description of the invention, it is to be understood that the orientation or position of the instruction such as term " on ", " under ", " interior ", " outer "
It is based on orientation shown in the drawings or position relationship to put relation, is for only for ease of the description present invention and simplifies description, rather than
Indicate or imply that signified device or element there must be specific orientation, with specific azimuth configuration and operation, therefore not
It is understood that as limitation of the present invention.
In the present invention, unless otherwise clearly defined and limited, the term such as term " connection " should be interpreted broadly, example
Such as, can be fixedly connected or be detachably connected, or integrally；Can be mechanical connection or electrical connection；
Can make to be joined directly together, can also be indirectly connected by intermediary, can be two element internals connection or two elements
Interaction relationship.For the ordinary skill in the art, above-mentioned term can be understood at this as the case may be
Concrete meaning in invention.In addition, term " comprising " and " having " and their any deformation, it is intended that covering is non-exclusive
Include, be not necessarily limited to clearly arrange for example, containing the process of series of steps or unit, method, apparatus, product or equipment
Those steps or unit gone out, but may include not list clearly or consolidate for these processes, method, product or equipment
The other steps or unit having.
Embodiment one
A kind of separate type system for pretreating sample is present embodiments provided, the system is applied to the processing of whole blood sample.
As shown in Figures 1 to 4, the separate type system for pretreating sample that the present embodiment provides includes：
Sample disk module, as shown in figure 5, including sample disk 1 and the first rotation driving being arranged on below the sample disk 1
Mechanism 2, first rotary drive mechanism 2 are used to drive the sample disk 1 to rotate.Preferably, the sample disk 1 is disk
Shape, the sample disk 1 are circumferentially equidistantly arranged with 25 sample tube seats 3, and a sample is placed with each sample tube seat 3
This pipe, each it is loaded with blood sample described in 50-150 μ L in the sample tube.
Antibody disk module, as shown in fig. 6, including antibody disk 4 and the second rotation driving being arranged on below the antibody disk 4
Mechanism 5, second rotary drive mechanism 5 are used to drive the antibody disk 4 to rotate.Preferably, the antibody disk 4 is disk
Shape, the antibody disk 4 are circumferentially equidistantly arranged with 25 antibody tube seats 6, and being placed with one in each antibody tube seat 6 resists
Body pipe, each it is loaded with 5-25 μ L antibody in the antibody pipe.Preferably, the outside of the antibody disk 4 is provided with the first temperature control
Chamber 29, first temperature control cavity 29 is used to make the temperature of the antibody disk 4 maintain 2~8 DEG C, so as to extend making for the antibody
Use the life-span.
First liquid relief arm module, including the first probe 7, the first mechanical arm 8 for fixing first probe 7, be used for
Drive the first drive mechanism 9 that the first mechanical arm 8 rotates and second for driving the up and down motion of first mechanical arm 8
Drive mechanism 10.The first mechanical arm 8 is driven with first drive mechanism 9 and described second successively by first rotating shaft 26
Mechanism 10 connects, and the first rotating shaft 26 is located at the right side of the sample disk 1 and the antibody disk 4, and the first rotating shaft 26
The distance between described center of sample disk 1 and the first rotating shaft 26 are equal with the distance between the center of antibody disk 4.It is excellent
Choosing, the first mechanical arm 8 has positional fault warning function, when position occurs in the motion process of first mechanical arm 8
During mistake, alarm signal is sent.First probe 7 is applied not only to draw the antibody and the blood sample, is additionally operable to quantitative
Fill the antibody and the blood sample.First probe 7 has anti-collision, when first probe 7 is by vertical
It can be automatically stopped when the resistance in direction or shock, and send alarm signal.It is worth noting that, the internal diameter of first probe 7
Should not it is too big also should not be too small, if excessive, the precision of antibody described in compressing can be caused to be forbidden；If too small, can cause described in compressing
Less cleaning position after blood sample and stifled pin.In order to avoid the antibody wastes, the first liquid level is provided with the first mechanical arm 8
Sensor (not shown), for the liquid level of the antibody in antibody pipe described in automatic detection, ensure that the antibody is maximum
Degree blots.
Second liquid relief arm module, including the second probe 11, the second mechanical arm 12 for fixing second probe 11, use
In the 3rd drive mechanism 13 for driving the rotation of second mechanical arm 12 and for driving the second mechanical arm 12 to move up and down
The 4th drive mechanism.The second mechanical arm 12 by the second rotating shaft 27 successively with the 3rd drive mechanism 13 and described
Four drive mechanisms connect, and second rotating shaft 27 is located at the right back of first rotating shaft 26.Preferably, the second mechanical arm 12
With positional fault warning function, when positional fault occurs in the motion process of second mechanical arm 12, alarm signal is sent.
Second probe 11 is used to fill hemolysin and PBS solution.Second probe 11 has anti-collision, when described second
Probe 11 by vertical direction resistance or shock when can be automatically stopped, and send alarm signal.
Disk module is centrifuged, as shown in fig. 7, comprises centrifugal pan 14 and the 3rd rotation being arranged on below the centrifugal pan 14 are driven
Motivation structure 15.The centrifugal pan 14 is discoid, and the centrifugal pan 14 is circumferentially equidistantly arranged with 24 reaction pipe holders 16,
Two reaction tube seats 17 are provided with each reaction pipe holder 16, a reaction is placed with each reaction tube seat 17
Pipe, the reaction solution that the blood sample, the antibody and the hemolysin are formed each is loaded with the reaction tube.In this implementation
In example, the volume of reaction solution is 2mL in the reaction tube.The incubation reaction of the reaction solution is completed in the reaction tube.Institute
Centrifugal pan 14 is stated by the vibrations of itself and fills the liquid impulse force of hemolysin and makes the blood sample, the antibody and described molten
Sanguinin mixes, easy to operate, and cost is relatively low.3rd rotary drive mechanism 15 is suitable for driving the centrifugal pan 14 to realize
Hour hands rotate counterclockwise, and in the present embodiment, the rotating speed of the centrifugal pan 14 is 0-2500r/min.3rd rotation is driven
Motivation structure 15 is additionally operable to drive the realization of centrifugal pan 14 to be accurately positioned, so as to which specified reaction tube to be stopped to the position specified,
To coordinate first probe 7 and second probe 11 to complete pipetting.Preferably, the 3rd rotary drive mechanism 15
Side is provided with cushioning support-saddle 25, and when the centrifugal pan 14 centrifuges clockwise or counterclockwise, the cushioning support-saddle 25 can
To reduce the vibration amplitude of the centrifugal pan 14, so as to ensure that the positioning of the centrifugal pan 14 is more accurate.The centrifugal pan 14
Outside be provided with the second temperature control cavity 30, the setting of second temperature control cavity 30 is to ensure the reaction temperature of the centrifugal pan 14
Degree maintains 37 DEG C, makes it closer to the temperature of human body.
3rd liquid relief arm module, including the 3rd probe 18, the three-mechanical arm 19 for fixing the 3rd probe 18, use
In the 5th drive mechanism 20 for driving the rotation of three-mechanical arm 19 and for driving the three-mechanical arm 19 to move up and down
The 6th drive mechanism 21.The three-mechanical arm 19 by the 3rd rotating shaft 28 successively with the 5th drive mechanism 20 and described
6th drive mechanism 21 connects, and the 3rd rotating shaft 28 is located at the right side of second rotating shaft 27.Preferably, the 3rd machinery
Arm 19 has positional fault warning function, when positional fault occurs in the motion process of three-mechanical arm 19, sends alarm
Signal.3rd probe 18 is used to aspirate the supernatant after centrifugation.3rd probe 18 has anti-collision, when described
3rd probe 18 by vertical direction resistance or shock when can be automatically stopped, and send alarm signal.Preferably, the described 3rd
The second liquid level sensor (not shown) is provided with mechanical arm 19, second liquid level sensor is used for described in automatic detection
The liquid level of supernatant in reaction tube, ensure that the 3rd probe 18 possesses delineation pumping function under side when sucting clear liquid,
Avoid the 3rd probe 18 and the supernatant contact too deep, cause the 3rd probe 18 in the bad cleaning of service sink.
Preferably, first drive mechanism 9, second drive mechanism 10, the 3rd drive mechanism 13, described
Four drive mechanisms, the 5th drive mechanism 20 and the 6th drive mechanism 21 are Walk stepper motors, and first rotation is driven
Motivation structure 2, second rotary drive mechanism 5 and the 3rd rotary drive mechanism 15 are servomotor.
Cleaning module, the cleaning module include cleaning pump (not shown), syringe pump (not shown), magnetic valve
(not shown), fluid path pipeline (not shown), service sink, cleaning fluid bucket (not shown) and waste liquid barrel are (in figure not
Show), first probe 7, second probe 11 and the 3rd probe 18 are connected with the syringe pump respectively, can be passed through
The absorption and discharge of the antibody, the blood sample, the hemolysin and the PBS solution are completed in the control of the syringe pump,
The syringe pump is connected by the magnetic valve with the cleaning pump, and the cleaning pump intake is connected with the cleaning fluid bucket, institute
State cleaning pump discharge to be connected with the service sink by the fluid path pipeline, the service sink is connected with waste liquid barrel.
Wherein, the service sink includes the first service sink 22, the second service sink 23 and the 3rd service sink 24, and described first is clear
Wash pool 22 is arranged on the lower section of first probe 7, and second service sink 23 is arranged on the lower section of second probe 11, institute
State the lower section that the 3rd service sink 24 is arranged on the 3rd probe 18.First probe 7 will return described after each
First service sink 22 cleans once, and second probe 11 will return second service sink clear 23 and wash one after each
Secondary, the 3rd probe 18 will return the 3rd service sink 24 and clean once after each, so as to keep away to greatest extent
Exempt from cross pollution, ensure the control of cross pollution rate within 0.05%.
The separate type system for pretreating sample also includes operational control module, and the operational control module is used to control institute
State sample disk module, the antibody disk module, the first liquid relief arm module, the second liquid relief arm module, the 3rd shifting
The action of liquid arm module and the centrifugation disk module.Specifically, the operational control module includes touch-screen 31 and control calculates
Machine, the control computer provide control operation for each module in the separate type system for pretreating sample, whole to control
Sample process process.
One sample process process comprises the following steps：Add antibody → heal sample → incubation → mixing → solubilization sanguinin →
Centrifuging → draw up clear liquid, → plus PBS solution → centrifuges → drawn up that clear liquid → plus PBS solution → centrifuges → drawn up clear liquid → plus PBS is molten
Liquid.
Preferably, the sample disk module, the antibody disk module, the first liquid relief arm module, second liquid relief
Arm module, the 3rd liquid relief arm module and the centrifugation disk module are integrated on an instrument host 32, the touch-screen 31
It is arranged in the cabinet of the instrument host 32.
Preferably, rail plate (not shown), the touch-screen 31 are provided with the cabinet of the instrument host 32
It is connected with the rail plate, the touch-screen 31 is extracted out or is pushed into the cabinet of the instrument host 32, it is convenient to carry out
Operation.
Embodiment two
A kind of separate type system for pretreating sample is present embodiments provided, the system is applied to the processing of whole blood sample.
As shown in Figures 1 to 4, the separate type system for pretreating sample that the present embodiment provides includes：
Sample disk module, as shown in figure 5, including sample disk 1 and the first rotation driving being arranged on below the sample disk 1
Mechanism 2, first rotary drive mechanism 2 are used to drive the sample disk 1 to rotate.Preferably, the sample disk 1 is disk
Shape, the sample disk 1 are circumferentially equidistantly arranged with 25 sample tube seats 3, and a sample is placed with each sample tube seat 3
This pipe, each it is loaded with blood sample described in 50-150 μ L in the sample tube.
Antibody disk module, as shown in fig. 6, including antibody disk 4 and the second rotation driving being arranged on below the antibody disk 4
Mechanism 5, second rotary drive mechanism 5 are used to drive the antibody disk 4 to rotate.Preferably, the antibody disk 4 is disk
Shape, the antibody disk 4 are circumferentially equidistantly arranged with 25 antibody tube seats 6, and being placed with one in each antibody tube seat 6 resists
Body pipe, each it is loaded with 5-25 μ L antibody in the antibody pipe.Preferably, the outside of the antibody disk 4 is provided with the first temperature control
Chamber 29, first temperature control cavity 29 is used to make the temperature of the antibody disk 4 maintain 2~8 DEG C, so as to extend making for the antibody
Use the life-span.
First liquid relief arm module, including the first probe 7, the first mechanical arm 8 for fixing first probe 7, be used for
Drive the first drive mechanism 9 that the first mechanical arm 8 rotates and second for driving the up and down motion of first mechanical arm 8
Drive mechanism 10.The first mechanical arm 8 is driven with first drive mechanism 9 and described second successively by first rotating shaft 26
Mechanism 10 connects, and the first rotating shaft 26 is located at the right side of the sample disk 1 and the antibody disk 4, and the first rotating shaft 26
The distance between described center of sample disk 1 and the first rotating shaft 26 are equal with the distance between the center of antibody disk 4.It is excellent
Choosing, the first mechanical arm 8 has positional fault warning function, when position occurs in the motion process of first mechanical arm 8
During mistake, alarm signal is sent.First probe 7 is applied not only to draw the antibody and the blood sample, is additionally operable to quantitative
Fill the antibody and the blood sample.First probe 7 has anti-collision, when first probe 7 is by vertical
It can be automatically stopped when the resistance in direction or shock, and send alarm signal.It is worth noting that, the internal diameter of first probe 7
Should not it is too big also should not be too small, if excessive, the precision of antibody described in compressing can be caused to be forbidden；If too small, can cause described in compressing
Less cleaning position after blood sample and stifled pin.In order to avoid the antibody wastes, the first liquid level is provided with the first mechanical arm 8
Sensor (not shown), for the liquid level of the antibody in antibody pipe described in automatic detection, ensure that the antibody is maximum
Degree blots.
Second liquid relief arm module, including the second probe 11, the second mechanical arm 12 for fixing second probe 11, use
In the 3rd drive mechanism 13 for driving the rotation of second mechanical arm 12 and for driving the second mechanical arm 12 to move up and down
The 4th drive mechanism.The second mechanical arm 12 by the second rotating shaft 27 successively with the 3rd drive mechanism 13 and described
Four drive mechanisms connect, and second rotating shaft 27 is located at the right back of first rotating shaft 26.Preferably, the second mechanical arm 12
With positional fault warning function, when positional fault occurs in the motion process of second mechanical arm 12, alarm signal is sent.
Second probe 11 is used to fill hemolysin and PBS solution.Second probe 11 has anti-collision, when described second
Probe 11 by vertical direction resistance or shock when can be automatically stopped, and send alarm signal.
Disk module is centrifuged, as shown in fig. 7, comprises centrifugal pan 14 and the 3rd rotation being arranged on below the centrifugal pan 14 are driven
Motivation structure 15.The centrifugal pan 14 is discoid, and the centrifugal pan 14 is circumferentially equidistantly arranged with 24 reaction pipe holders 16,
Two reaction tube seats 17 are provided with each reaction pipe holder 16, a reaction is placed with each reaction tube seat 17
Pipe, the reaction solution that the blood sample, the antibody and the hemolysin are formed each is loaded with the reaction tube.In this implementation
In example, the volume of reaction solution is 2mL in the reaction tube.The incubation reaction of the reaction solution is completed in the reaction tube.Institute
Centrifugal pan 14 is stated by the vibrations of itself and fills the liquid impulse force of hemolysin and makes the blood sample, the antibody and described molten
Sanguinin mixes, easy to operate, and cost is relatively low.3rd rotary drive mechanism 15 is suitable for driving the centrifugal pan 14 to realize
Hour hands rotate counterclockwise, and in the present embodiment, the rotating speed of the centrifugal pan 14 is 0-2500r/min.3rd rotation is driven
Motivation structure 15 is additionally operable to drive the realization of centrifugal pan 14 to be accurately positioned, so as to which specified reaction tube to be stopped to the position specified,
To coordinate first probe 7 and second probe 11 to complete pipetting.Preferably, the 3rd rotary drive mechanism 15
Side is provided with cushioning support-saddle 25, and when the centrifugal pan 14 centrifuges clockwise or counterclockwise, the cushioning support-saddle 25 can
To reduce the vibration amplitude of the centrifugal pan 14, so as to ensure that the positioning of the centrifugal pan 14 is more accurate.The centrifugal pan 14
Outside be provided with the second temperature control cavity 30, the setting of second temperature control cavity 30 is to ensure the reaction temperature of the centrifugal pan 14
Degree maintains 37 DEG C, makes it closer to the temperature of human body.
3rd liquid relief arm module, including the 3rd probe 18, the three-mechanical arm 19 for fixing the 3rd probe 18, use
In the 5th drive mechanism 20 for driving the rotation of three-mechanical arm 19 and for driving the three-mechanical arm 19 to move up and down
The 6th drive mechanism 21.The three-mechanical arm 19 by the 3rd rotating shaft 28 successively with the 5th drive mechanism 20 and described
6th drive mechanism 21 connects, and the 3rd rotating shaft 28 is located at the right side of second rotating shaft 27.Preferably, the 3rd machinery
Arm 19 has positional fault warning function, when positional fault occurs in the motion process of three-mechanical arm 19, sends alarm
Signal.3rd probe 18 is used to aspirate the supernatant after centrifugation.3rd probe 18 has anti-collision, when described
3rd probe 18 by vertical direction resistance or shock when can be automatically stopped, and send alarm signal.Preferably, the described 3rd
The second liquid level sensor (not shown) is provided with mechanical arm 19, second liquid level sensor is used for described in automatic detection
The liquid level of supernatant in reaction tube, ensure that the 3rd probe 18 possesses delineation pumping function under side when sucting clear liquid,
Avoid the 3rd probe 18 and the supernatant contact too deep, cause the 3rd probe 18 in the bad cleaning of service sink.
Preferably, first drive mechanism 9, second drive mechanism 10, the 3rd drive mechanism 13, described
Four drive mechanisms, the 5th drive mechanism 20 and the 6th drive mechanism 21 are Walk stepper motors, and first rotation is driven
Motivation structure 2, second rotary drive mechanism 5 and the 3rd rotary drive mechanism 15 are servomotor.
Cleaning module, the cleaning module include cleaning pump (not shown), syringe pump (not shown), magnetic valve
(not shown), fluid path pipeline (not shown), service sink, cleaning fluid bucket (not shown) and waste liquid barrel are (in figure not
Show), first probe 7, second probe 11 and the 3rd probe 18 are connected with the syringe pump respectively, can be passed through
The absorption and discharge of the antibody, the blood sample, the hemolysin and the PBS solution are completed in the control of the syringe pump,
The syringe pump is connected by the magnetic valve with the cleaning pump, and the cleaning pump intake is connected with the cleaning fluid bucket, institute
State cleaning pump discharge to be connected with the service sink by the fluid path pipeline, the service sink is connected with waste liquid barrel.
Wherein, the service sink includes the first service sink 22, the second service sink 23 and the 3rd service sink 24, and described first is clear
Wash pool 22 is arranged on the lower section of first probe 7, and second service sink 23 is arranged on the lower section of second probe 11, institute
State the lower section that the 3rd service sink 24 is arranged on the 3rd probe 18.First probe 7 will return described after each
First service sink 22 cleans once, and second probe 11 will return second service sink clear 23 and wash one after each
Secondary, the 3rd probe 18 will return the 3rd service sink 24 and clean once after each, so as to keep away to greatest extent
Exempt from cross pollution, ensure the control of cross pollution rate within 0.05%.
The separate type system for pretreating sample also includes operational control module, and the operational control module is used to control institute
State sample disk module, the antibody disk module, the first liquid relief arm module, the second liquid relief arm module, the 3rd shifting
The action of liquid arm module and the centrifugation disk module.Specifically, the operational control module includes touch-screen 31 and control calculates
Machine, the control computer provide control operation for each module in the separate type system for pretreating sample, whole to control
Sample process process.
One sample process process comprises the following steps：Add antibody → heal sample → incubation → mixing → solubilization sanguinin →
Centrifuging → draw up clear liquid, → plus PBS solution → centrifuges → drawn up that clear liquid → plus PBS solution → centrifuges → drawn up clear liquid → plus PBS is molten
Liquid.
Preferably, the sample disk module, the antibody disk module, the first liquid relief arm module, second liquid relief
Arm module, the 3rd liquid relief arm module and the centrifugation disk module are integrated on an instrument host 32, the touch-screen 31
It is arranged in the cabinet of the instrument host 32.
Preferably, rail plate (not shown), the touch-screen 31 are provided with the cabinet of the instrument host 32
It is connected with the rail plate, the touch-screen 31 is extracted out or is pushed into the cabinet of the instrument host 32, it is convenient to carry out
Operation.
The separate type system for pretreating sample also includes scan sample module (not shown), the scan sample mould
Block is arranged on the outside of the sample tube, and is connected with the operational control module, can be put into by scanning on the outside of the sample tube
Quick Response Code, feed back to the operational control module, the experiment flow of the sample tube controlled by the operational control module.
Separate type system for pretreating sample provided by the invention, it is complete the shortcomings that can be good at overcoming artificial treatment to bring
The cell preparation work of blood sample before machine is analyzed on into flow cytometer, it can largely save artificial and can and improve at blood sample
Manage effect.
Described above is only the preferred embodiments of the present invention, based on the embodiment in the present invention, ordinary skill people
The every other embodiment that member is obtained under the premise of creative work is not made, it should all belong to the model that the present invention protects
Enclose.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, may be used also
To make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
- A kind of 1. separate type system for pretreating sample, it is characterised in that including：Sample disk module, including sample disk (1) and the first rotary drive mechanism (2) for driving the sample disk (1) to rotate, The sample disk (1) is circumferentially equidistantly arranged with multiple sample tube seats (3)；Antibody disk module, including antibody disk (4) and the second rotary drive mechanism (5) for driving antibody disk (4) rotation, The antibody disk (4) is circumferentially equidistantly arranged with multiple antibody tube seats (6)；First liquid relief arm module, including the first probe (7), the first mechanical arm (8) for fixing first probe (7), use In the first drive mechanism (9) for driving first mechanical arm (8) rotation and for driving the first mechanical arm (8) to transport up and down Dynamic the second drive mechanism (10), first probe (7) are used to draw and fill antibody and sample；Second liquid relief arm module, including the second probe (11), the second mechanical arm (12) for fixing second probe (11), For driving the 3rd drive mechanism (13) of the second mechanical arm (12) rotation and for driving the second mechanical arm (12) The 4th drive mechanism to move up and down, second probe (11) are used to fill lysate and buffer solution；Centrifuge disk module, including centrifugal pan (14) and the 3rd rotary drive mechanism for driving the centrifugal pan (14) to rotate (15), the centrifugal pan (14) is circumferentially equidistantly arranged with multiple reaction pipe holders (16), each reaction pipe holder (16) it is provided with least one reaction tube seat (17) on, the centrifugal pan (14) is used to realizing the sample, antibody, described Incubation, mixing and the centrifugation of lysate and the buffer solution, the 3rd rotary drive mechanism (15) are used to drive the centrifugation Disk (14) is realized and rotates clockwise or counterclockwise and be accurately positioned；3rd liquid relief arm module, including the 3rd probe (18), the three-mechanical arm (19) for fixing the 3rd probe (18), For driving the 5th drive mechanism (20) of three-mechanical arm (19) rotation and for driving the three-mechanical arm (19) The 6th drive mechanism (21) to move up and down, the 3rd probe (18) are used to aspirate the supernatant after centrifugation.
- 2. separate type system for pretreating sample according to claim 1, it is characterised in that the separate type Sample pretreatment System also includes operational control module, the operational control module be used to controlling the sample disk module, the antibody disk module, The first liquid relief arm module, the second liquid relief arm module, the 3rd liquid relief arm module and the centrifugation disk module it is dynamic Make.
- 3. separate type system for pretreating sample according to claim 1, it is characterised in that the separate type Sample pretreatment System also includes cleaning module, and the cleaning module includes cleaning pump, syringe pump, magnetic valve, fluid path pipeline, service sink and cleaning Liquid bucket, first probe (7), second probe (11) and the 3rd probe (18) are connected with the syringe pump respectively, The syringe pump is connected by the magnetic valve with the cleaning pump, and the cleaning pump intake is connected with the cleaning fluid bucket, institute Cleaning pump discharge is stated to be connected with the service sink by the fluid path pipeline.
- 4. separate type system for pretreating sample according to claim 3, it is characterised in that it is clear that the service sink includes first Wash pool (22), the second service sink (23) and the 3rd service sink (24), first service sink (22) are arranged on first probe (7) lower section, second service sink (23) are arranged on below second probe (11), and the 3rd service sink (24) is arranged on Below 3rd probe (18).
- 5. separate type system for pretreating sample according to claim 1, it is characterised in that the centrifugal pan (14) is by certainly The vibrations of body and the liquid impulse force of the filling lysate mix the sample, the antibody and the lysate.
- 6. separate type system for pretreating sample according to claim 1, it is characterised in that the 3rd rotary drive mechanism (15) side is provided with the cushioning support-saddle (25) for reducing the centrifugal pan (14) vibration amplitude.
- 7. separate type system for pretreating sample according to claim 1, it is characterised in that the first mechanical arm (8) is logical Cross first rotating shaft (26) to be connected with first drive mechanism (9) and second drive mechanism (10) successively, described first turn Between the distance between axle (26) and the sample disk (1) center and the first rotating shaft (26) and the antibody disk (4) center Distance it is equal.
- 8. separate type system for pretreating sample according to claim 1, it is characterised in that on the first mechanical arm (8) The first liquid level sensor is provided with, the liquid level that first liquid level sensor is used for antibody in antibody pipe described in automatic detection is high Spend, be provided with the second liquid level sensor on the three-mechanical arm (19), second liquid level sensor is used for automatic detection institute State the liquid level of supernatant in reaction tube.
- 9. separate type system for pretreating sample according to claim 1, it is characterised in that the outside of the antibody disk (4) The first temperature control cavity (29) is provided with, first temperature control cavity (29) is used to make the temperature of the antibody disk (4) maintain 2~8 DEG C； The outside of the centrifugal pan (14) is provided with the second temperature control cavity (30), and second temperature control cavity (30) is used to make the centrifugal pan (14) reaction temperature maintains 37 DEG C.
- 10. separate type system for pretreating sample according to claim 1, it is characterised in that pair of the sample tube seat (3) Answer and sample tube is placed with position, the sample tube is used to contain the sample；Put on the correspondence position of the antibody tube seat (6) Antibody pipe is equipped with, the antibody pipe is used to contain the antibody；Reaction is placed with the correspondence position of the reaction tube seat (17) Manage, the reaction solution that the sample, the antibody and the lysate are formed is loaded with the reaction tube.
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| CN201710689588.XA CN107340171A (en) | 2017-08-14 | 2017-08-14 | A kind of separate type system for pretreating sample |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710689588.XA CN107340171A (en) | 2017-08-14 | 2017-08-14 | A kind of separate type system for pretreating sample |
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| CN201710689588.XA Pending CN107340171A (en) | 2017-08-14 | 2017-08-14 | A kind of separate type system for pretreating sample |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111610282A (en) * | 2020-07-16 | 2020-09-01 | 生态环境部南京环境科学研究所 | Device and method for detecting residual quantity of abamectin B2a in water body |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4234538A (en) * | 1977-10-28 | 1980-11-18 | Coulter Electronics, Inc. | Apparatus for monitoring chemical reactions and employing moving photometer means |
| US4961906A (en) * | 1984-04-12 | 1990-10-09 | Fisher Scientific Company | Liquid handling |
| US5855851A (en) * | 1996-07-19 | 1999-01-05 | Hitachi, Ltd. | Apparatus for trasferring liquid having liquid level sensing function |
| JP2000074927A (en) * | 1998-08-31 | 2000-03-14 | Sysmex Corp | Liquid sucking device |
| US6148666A (en) * | 1997-10-29 | 2000-11-21 | Boehringer Mannheim Gmbh | Method and device for liquid transfer with an analysis apparatus |
| CN1963527A (en) * | 2005-11-10 | 2007-05-16 | 深圳迈瑞生物医疗电子股份有限公司 | Full-automatic biochemical analyzer and analysis method thereof |
| CN104001563A (en) * | 2014-06-09 | 2014-08-27 | 上海优爱宝机器人技术有限公司 | Pipettor |
| CN204034757U (en) * | 2014-08-28 | 2014-12-24 | 武汉中地西能科技有限公司 | A kind of pipette Rapid Cleaning and get liquid servicing unit |
| CN106018045A (en) * | 2016-07-04 | 2016-10-12 | 邱加伟 | Pipetting mechanism for sectioning and staining cells |
-
2017
- 2017-08-14 CN CN201710689588.XA patent/CN107340171A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4234538A (en) * | 1977-10-28 | 1980-11-18 | Coulter Electronics, Inc. | Apparatus for monitoring chemical reactions and employing moving photometer means |
| US4961906A (en) * | 1984-04-12 | 1990-10-09 | Fisher Scientific Company | Liquid handling |
| US5855851A (en) * | 1996-07-19 | 1999-01-05 | Hitachi, Ltd. | Apparatus for trasferring liquid having liquid level sensing function |
| US6148666A (en) * | 1997-10-29 | 2000-11-21 | Boehringer Mannheim Gmbh | Method and device for liquid transfer with an analysis apparatus |
| JP2000074927A (en) * | 1998-08-31 | 2000-03-14 | Sysmex Corp | Liquid sucking device |
| CN1963527A (en) * | 2005-11-10 | 2007-05-16 | 深圳迈瑞生物医疗电子股份有限公司 | Full-automatic biochemical analyzer and analysis method thereof |
| CN104001563A (en) * | 2014-06-09 | 2014-08-27 | 上海优爱宝机器人技术有限公司 | Pipettor |
| CN204034757U (en) * | 2014-08-28 | 2014-12-24 | 武汉中地西能科技有限公司 | A kind of pipette Rapid Cleaning and get liquid servicing unit |
| CN106018045A (en) * | 2016-07-04 | 2016-10-12 | 邱加伟 | Pipetting mechanism for sectioning and staining cells |
Non-Patent Citations (1)
| Title |
|---|
| 吕申 等: "《病理检验技术》", 人民军医出版社, pages: 157 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111610282A (en) * | 2020-07-16 | 2020-09-01 | 生态环境部南京环境科学研究所 | Device and method for detecting residual quantity of abamectin B2a in water body |
| CN111610282B (en) * | 2020-07-16 | 2022-07-12 | 生态环境部南京环境科学研究所 | Device and method for detecting residual quantity of abamectin B2a in water body |
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