CN107338192B

CN107338192B - Biological cell culture real-time radio frequency acquisition system

Info

Publication number: CN107338192B
Application number: CN201710586261.XA
Authority: CN
Inventors: 李娅; 殷海荣; 徐进; 桂文超
Original assignee: University of Electronic Science and Technology of China
Current assignee: University of Electronic Science and Technology of China
Priority date: 2017-07-18
Filing date: 2017-07-18
Publication date: 2023-09-08
Anticipated expiration: 2037-07-18
Also published as: CN107338192A

Abstract

The invention discloses a real-time radio frequency acquisition system for biological cell culture, and belongs to the field of combination of biological cell culture and a microwave circuit. The system comprises a medium substrate, input end connectors and output end connectors which are arranged at two ends of the medium substrate, a metal grounding plate which covers the back surface of the medium substrate, a one-to-many power distribution feed network which is arranged at the front surface of the medium substrate, a plurality of microstrip lines which are arranged in parallel and a microchannel. The micro-channel is arranged in the middle of the microstrip line, separates the microstrip line into two parts and forms a microstrip capacitor; the microwave source feeds the microstrip line input end through the input end connector and the one-to-many power division feed network, the microstrip line output end outputs microwave signals through the output end connector, and the real-time growth condition of cells can be obtained through analysis and calculation of the microwave signals. The invention can collect data while culturing, saves time and labor, and has simple operation and high monitoring efficiency. It also has the advantage of miniaturization.

Description

Biological cell culture real-time radio frequency acquisition system

Technical Field

The invention belongs to the field of combination of biological cell culture and a microwave circuit, and particularly relates to a biological cell culture real-time radio frequency acquisition system.

Background

Cell culture is a rapidly developing experimental technique in modern biological science, which makes an important contribution to the study and application of cytology, genetics, virology and immunology. Conventional cell culture uses cell culture flasks, plates, etc. to culture cells. With the development of biotechnology, large-scale cell culture is urgently required. In the in vitro culture process of cells, conventional examination and observation are needed to know the growth state, quantity change, cell morphology and the like of the cells in time. Cell growth monitoring is an important component of biological cell culture technology.

Currently, there are a variety of techniques for analyzing cells. Conventional cell analysis techniques rely on optical monitoring systems. Conventional optical techniques require labeling of cells prior to observation with a microscope, and although more accurate images of cells can be obtained, the labeling and observation process requires a significant amount of time and expensive equipment, and labeling of cells can be detrimental to cells.

Compared with the common monitoring method for biological cell culture, the real-time radio frequency acquisition system for biological cell culture has the characteristics of no direct contact, real-time monitoring, convenient operation, innocuity, no need of marking cells and the like. The micro-channel is adopted to culture biological cells, so that the method has the advantages of miniaturization, saving of a large amount of reagents and cells and the like.

Disclosure of Invention

Aiming at the problems existing in the prior art, the invention provides a real-time radio frequency acquisition system for biological cell culture, which solves the technical problems that the monitoring efficiency is reduced due to higher monitoring cost and time consumption of the biological cell culture in the prior art, the cells need to be marked, and certain damage is possibly generated to the cell culture after the marking, and the like.

In order to achieve the above purpose, the invention adopts the following technical scheme:

the real-time radio frequency acquisition system for biological cell culture comprises a medium substrate, an input end connector, an output end connector, a metal grounding plate, a one-to-many power distribution feed network, a plurality of microstrip lines and a microchannel, wherein the input end connector and the output end connector are arranged at two ends of the medium substrate, the metal grounding plate is arranged on the back surface of the medium substrate, and the one-to-many power distribution feed network is arranged on the front surface of the medium substrate.

The micro-channel is arranged in the middle of the microstrip line, separates the microstrip line into two parts and forms a microstrip capacitor; the microwave source feeds the microstrip line input end through the input end connector and the one-to-many power division feed network, the microstrip line output end outputs microwave signals through the output end connector, and the real-time growth condition of cells can be obtained through analysis and calculation of the microwave signals.

Further, the microstrip line output end is communicated with the output end connector through the three-port microwave component.

Further, the number of the microstrip lines is 1 to 100.

In the process of cell culture, a microwave source is utilized to feed a one-to-many power divider feed network through an input end connector, the one-to-many power divider is connected with the same end of a plurality of microstrip lines which are arranged in parallel, and all the microstrip lines are divided into two parts by a micro-channel for cell culture. During the course of biological cell culture, the number of cells is almost zero at the beginning, and as time increases, the cells continuously transform the nutrient substances in the culture medium, and a plurality of new cells are generated by division. Thus, in this process, many substances are initially present in the form of individual macromolecules and finally in the form of cells. This difference in presence results in a change in the electrical parameters of the material throughout the microchannel, i.e., in both its dielectric constant and its electrical conductivity. When the electrical parameters of the materials in the micro-channel change, the capacitance of the microstrip capacitor also changes, and the coupling energy and the phase of the microstrip capacitor also change, and the changes are output through the output end connector. For these changes, algorithms can be applied to reverse infer the growth status of the cells.

Compared with the prior art, the invention has the beneficial effects that:

by introducing the biological cell culture real-time radio frequency acquisition system, the growth of the biological cells can be monitored in real time, and the monitored data can be acquired in real time. In the whole monitoring process, the method does not need to be in direct contact with cells or mark the cells, so the method does not harm the cells, and is simple and convenient to operate and high in monitoring efficiency.

By introducing the microchannel, the culture medium can be fully utilized, the contact between the cells and the culture medium is increased, and the cell culture device and the acquisition system are fused together, so that the method has the advantage of miniaturization. When monitoring biological cell culture, can cultivate the data of gathering simultaneously, labour saving and time saving.

Drawings

FIG. 1 is a schematic diagram showing the overall structural layout of example 1 of the present invention;

fig. 2 is a schematic diagram showing the overall structural layout of example 2 of the present invention.

Detailed Description

The invention will be further described by way of specific examples with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the invention more apparent.

Example 1

As shown in fig. 1, the real-time radio frequency acquisition system for biological cell culture comprises an input end connector 1_1, a one-to-six equal power division feed network 1_2, a microstrip line input end 1_3_1, a microstrip line output end 1_3_2, a microchannel 1_4, a three-port microwave component 1_5, an output end connector 1_6 and a dielectric substrate 1_7.

The metal floor covers the lower surface of the high-frequency dielectric substrate 1_7 and is completely overlapped with the bottom of the high-frequency dielectric substrate, so that good contact between the high-frequency dielectric substrate and the ground is ensured, and the high-frequency performance of the radio-frequency acquisition system is ensured. Six parallel arranged metal microstrip lines are printed on the upper surface of the high-frequency dielectric substrate 1_7, and a micro-channel is arranged in the middle of the microstrip lines to divide the microstrip lines into two parts which are bilaterally symmetrical, so that a microstrip capacitor with a rectangular structure is formed. The input end connector 1_1 is arranged at the leftmost side of the high-frequency medium substrate 1_7, and an inner core of the input end connector is connected with an input end of the one-to-six equal-power-division feed network 1_2 and is used for feeding the microstrip line input end 1_3_1, so that the feeding of each part of microstrip lines of the microstrip line input end 1_3_1 is guaranteed to be in the same direction with the same amplitude. The three-port microwave component 1_5 connected behind the microstrip line output end 1_3_2 is used for converting signals on the line after passing through the microstrip line output end 1_3_2 to reach the output end. The output terminal connector 1_6 is mounted on the rightmost side of the dielectric substrate 1_7, and is used for outputting signals converted by the three-port microwave component 1_5. The microstrip capacitance is used for reflecting the growth of biological cells, and in the process of culturing biological cells, the cells continuously convert the nutrient substances in the micro-channel 1_4 in a division manner along with the increase of culture time, so that the existence modes of substances in the micro-channel 1_4 are different, and the existence modes of the substances in the micro-channel 1_4 are different, so that the electric parameters of the substances in the whole micro-channel 1_4 are changed, namely, the dielectric constant and the electric conductivity of the substances are changed. When the electrical parameters of the substance in the microchannel 1_4 change, the capacitance of the microstrip capacitor changes, and the coupling energy and phase thereof also change. For these changes, algorithms can be applied to reverse infer the growth status of the cells.

Example 2

As shown in fig. 2, the real-time radio frequency acquisition system for biological cell culture comprises an input end connector 2_1, a one-to-three equal power division feed network 2_2, a microstrip line input end 2_3_1, a microstrip line output end 2_3_2, a microchannel 2_4, an output end connector 2_5 and a dielectric substrate 2_6.

The difference from example 1 is that: the microstrip line input terminal 2_3_1 and the microstrip line output terminal 2_3_2 in the present embodiment have a reduced number of microstrip lines, and three-port microwave components are eliminated. When the number of microstrip lines is small, the microwave signal is directly output from the output terminal connector 2_5 without converting the signal through the three-port microwave component, and this is basically the same as in embodiment 1. Except that finally, the present example uses an algorithm different from that of embodiment 1 to reverse the presumption of the growth status of the cells.

Claims

1. The real-time radio frequency acquisition system for biological cell culture comprises a medium substrate, an input end connector, an output end connector, a metal grounding plate, a one-to-many power distribution feed network, a plurality of microstrip lines and a microchannel, wherein the input end connector and the output end connector are arranged at two ends of the medium substrate;

the micro-channel is arranged in the middle of the micro-strip line, separates the micro-strip line into two parts which are symmetrical left and right, and forms a micro-strip capacitor; the microwave source feeds the microstrip line input end through the input end connector and the one-to-many power division feed network, the microstrip line output end outputs microwave signals through the output end connector, and the real-time growth condition of cells can be obtained through analysis and calculation of the microwave signals.

2. The biological cell culture real-time radio frequency acquisition system as claimed in claim 1, wherein: and the output end of the microstrip line is communicated with the output end connector through a three-port microwave component.

3. The biological cell culture real-time radio frequency acquisition system as claimed in claim 1, wherein: the number of the microstrip lines is 1-100.