CN107325319A - A kind of preparation method and application of porous Kynoar composite Nano Ag films - Google Patents
A kind of preparation method and application of porous Kynoar composite Nano Ag films Download PDFInfo
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Abstract
The invention discloses a kind of preparation method and application of porous Kynoar composite Nano Ag films, the preparation method includes：1）Suspension is prepared, by silver nitrate dispersing and dissolving in N, N dimethylformamides, Kynoar is added, carries out magnetic agitation；2）Casting solution is prepared, suspension is placed in shaking table, isothermal reaction；Reacted suspension is placed in standing and defoaming in vacuum drying oven；3）Film forming, cured film is obtained by casting solution by immersion precipitation phase inversion process；4）Cleaning；5）Dry, the cured film after cleaning is placed in vacuum drying oven and dried, porous Kynoar composite Nano Ag films are obtained.Obtained porous Kynoar composite Nano Ag films are used for manufacture of intraocular skin, with good anti-microbial property, and without obvious bio-toxicity, preparation method technique is simple, takes less, cost is low.
Description
Technical field
The present invention relates to biomaterial application field, and in particular to a kind of porous Kynoar composite Nano Ag films
Preparation method and application.
Background technology
Skin is the most important natural cover for defense of human body, can prevent invasion and the body of pathogenic microorganisms with internal organs in protective
Liquid loss etc..The skin wound that the reasons such as wound, burn, diabetic ulcer are caused, is highly prone to pathogenic micro- in agglutination
Biological invasion and attack and cause trauma surface infestation, suppuration, cause the even disunion of surface of a wound delayed union.Therefore, it is a kind of preferably artificial
Skin should possess good anti-microbial property, on the one hand can the sustainable protection surface of a wound do not attacked by extraneous pathogenic microorganisms, separately
On the one hand can also kill be colonized in the surface of a wound bacterium prevent infection further send out, final wound healing, recover skin
The complete function of skin.
The conventional antibiotic preparation of clinic is antibiotic, but causes bacterial resistance to be asked due to the abuse of antibiotic in recent years
Topic constantly aggravation, and thus expedited the emergence of a variety of multi-drug resistant bacterias, such as methicillin-resistant staphylococcus aureus, Bao Man not levers
Bacterium, pseudomonas aeruginosa etc., seriously threaten human health.So, the antibiotic preparation that substitute antibiotics are capable of in searching is very necessary.
Research confirms that Nano Silver is difficult the influence of acceptor internal and external factor, antibiotics resistance problem can be avoided well.Nano Silver is main
By destroying bacterial membrane, DNA and disturbing the activity of bacterial respiratory chain enzyme to kill bacterium.Its particle diameter is small, specific surface area
Greatly, to gram-negative bacteria and gram positive bacteria, it is respectively provided with good bactericidal action；Meanwhile, Nano Silver also has anti-inflammatory analgesic, promoted
Enter the effect of wound healing.
Kynoar has excellent mechanical performance, chemical stability and heat endurance.Simultaneously it is nontoxic, harmless, without cause
Abnormal effect, with good biocompatibility.Kynoar is cheap, and easily batch machining production.Therefore, it is one
Plant the timbering material of excellent structure artificial skin.
It is presently used for preparing the chemical reduction method complex operation of Nano Silver, takes time and effort.In addition, how Nano Silver to be had
Effect ground is combined with Kynoar also faces certain challenge to prepare antibacterial film.
The content of the invention
It is an object of the invention to provide a kind of preparation method and application of porous Kynoar composite Nano Ag films, its
Operation is simple, and obtained film thickness is controllable, and good biocompatibility, antibacterial effect is good.
The preparation method of porous Kynoar composite Nano Ag films of the present invention, comprises the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 10-60min under conditions of 1000-1500rpm for 40-80 DEG C and mixing speed, obtains suspension, the suspension
The concentration of silver nitrate is 0.1 ~ 1wt% in liquid, and the concentration of Kynoar is 5 ~ 20wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, temperature be 25-60 DEG C and rotating speed be 50-
12-72h, then 1-6h of standing and defoaming under conditions of temperature is 30-50 DEG C are reacted under conditions of 150rpm, casting solution is obtained；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 100-1000 μm, then will
10-60min of standing in deionized water is placed in for the mould of 100-1000 μm of casting solution containing thickness, cured film is obtained；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, is done under conditions of temperature is 30-50 DEG C
Dry 8-16h, obtains porous Kynoar composite Nano Ag films, the aperture of the film is 0.5-2.5 μm.
Further, the step 1）The concentration of silver nitrate is 0.425wt% in middle suspension, and the concentration of Kynoar is
10wt%。
Further, the step 1）In under conditions of temperature is 60 DEG C and mixing speed 1200rpm magnetic agitation
30min。
Further, the step 2）In be 37 DEG C in temperature and rotating speed is isothermal reaction 24h under conditions of 100rpm, in temperature
Spend for standing and defoaming 1h under conditions of 40 DEG C.
Further, the step 3）The material of middle mould is glass, polytetrafluoroethylene (PTFE) or polyester.
Further, the step 3）The middle thickness for controlling casting solution is 500 μm, then will contain the casting film that thickness is 500 μm
The mould of liquid, which is placed in deionized water, stands 30min, obtains cured film.
Further, the step 5）In temperature be 40 DEG C under conditions of dry 12h.
A kind of porous Kynoar composite Nano Ag films are in the artificial skin for preparing the defect of skin for treating people
Or the purposes in surgical dressing.
Further, the defect of skin is skin full-thickness defects or the skin infection surface of a wound.
Following performance test is carried out to the Kynoar composite Nano Ag films of the present invention.
1. scanning electron microscopic observation.
Referring to Fig. 1, obtained Kynoar composite nano silver is observed under different multiples using SEM
The surface texture of film.Using the softwares of Image-Pro Plus 6.0, Kynoar composite nano silver film surface is calculated
The aperture of hole is 0.5-1.5 μm.
2. transmission electron microscope observing.
Referring to Fig. 2, using Nano Silver in Kynoar composite Nano Ag films made from transmission electron microscope observation
Form and size.Observation understands that Nano Silver soilless sticking phenomenon in Kynoar is uniformly dispersed；Pass through Image J softwares
Measure a diameter of 1-5nm of nano-Ag particles.
The beneficial effects of the invention are as follows：
1st, silver nitrate and Kynoar are disperseed molten by the present invention using DMF as lytic agent and reducing agent
Solution is in DMF, and low whipping speed is magnetic agitation under conditions of 1000-1500rpm, can be in poly- inclined fluorine
Ethene situ generates Nano Silver, a diameter of 1-5nm of nano-Ag particles；By violent magnetic agitation, without adding pore-foaming agent
Carry out ultrasonic disperse, you can realize that Nano Silver is scattered in Kynoar, soilless sticking phenomenon by the preparation of Nano Silver and is received
A compound step for meter Yin Yu Kynoar is completed.
2nd, the present invention prepares porous Kynoar composite Nano Ag films by immersion precipitation phase inversion process, passes through N, N-
The interaction of dimethylformamide and deionized water, instantaneously carry out Phase separation, finally give characteristic containing space
Bag cavity-like structure, it is 0.5-1.5 μm of porous Kynoar composite Nano Ag films to prepare aperture, it is excessive if aperture if
The toughness of film can be reduced, and is easily attacked by bacterium, the gas permeability of film can be reduced if aperture is too small, it is influenceed in people
Application in work skin or in surgical dressing.
3rd, produced in preparation process of the invention without waste gas, waste liquid, it is environmentally friendly, and operation is simple, cost is low.
4th, by the polymer film prepared by immersion precipitation phase inversion process generally have various structures, the selection of polymer,
The concentration of polymer, the selection of solvent/non-solvent system, the structure and performance of the group pair of films of the composition of casting solution and coagulation bath
Influence is larger, and the present invention combines specific raw material proportioning and preparation technology, prepares aperture porous poly- inclined for 0.5-1.5 μm
PVF composite Nano Ag films, its thickness is controllable, and good biocompatibility, antibacterial effect is good, can be used in clinical drug-resistant bacterium sense
Contaminate the treatment of the surface of a wound.
Brief description of the drawings
Fig. 1 is that the SEM under Kynoar composite Nano Ag films different multiples prepared by the present invention is shone
Piece；
Fig. 2 is the transmission electron microscope photo of Kynoar composite Nano Ag films prepared by the present invention；
Fig. 3 is the cytoactive contrast block diagram of the porous Kynoar composite Nano Ag films prepared；
Fig. 4 is the absorbance contrast column of the anti-microbial property test experience of the porous Kynoar composite Nano Ag films prepared
Figure；
Fig. 5 is the Wound healing rate contrast block diagram of the zoopery of the porous Kynoar composite Nano Ag films prepared；
Fig. 6 is the wound healing time contrast column of the zoopery of the porous Kynoar composite Nano Ag films prepared
Figure.
Embodiment
The present invention is elaborated with reference to specific embodiment.
Embodiment one：A kind of preparation method of porous Kynoar composite Nano Ag films, comprises the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 30min under conditions of 1200rpm for 60 DEG C and mixing speed, obtains silver nitrate in suspension, the suspension
Concentration be 0.425wt%, the concentration of Kynoar is 10wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, and it is 100rpm's with rotating speed to be 37 DEG C in temperature
Under the conditions of react 24h, then in temperature be standing and defoaming 1h under conditions of 40 DEG C, obtain casting solution；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 500 μm, then will contain thickness
The mould spent for 500 μm of casting solution, which is placed in deionized water, stands 30min, obtains cured film；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, is dried under conditions of temperature is 40 DEG C
12h, obtains porous Kynoar composite Nano Ag films, the aperture of the film is 0.5-2.5 μm.
Embodiment two：A kind of preparation method of porous Kynoar composite Nano Ag films, comprises the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 10min under conditions of 1000rpm for 40 DEG C and mixing speed, obtains silver nitrate in suspension, the suspension
Concentration be 0.1wt%, the concentration of Kynoar is 5wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, temperature be 25 DEG C and rotating speed be under 50rpm it is permanent
Temperature reaction 12h, the standing and defoaming 1h under conditions of temperature is 30 DEG C, obtain casting solution；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 100 μm, then will contain thickness
The mould spent for 100 μm of casting solution, which is placed in deionized water, stands 10min, obtains cured film；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, and 8h is dried under conditions of temperature is 30 DEG C,
Porous Kynoar composite Nano Ag films are obtained, the aperture of the film is 0.5-2.5 μm.
Embodiment three：A kind of preparation method of porous Kynoar composite Nano Ag films, comprises the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 60min under conditions of 1500rpm for 80 DEG C and mixing speed, obtains silver nitrate in suspension, the suspension
Concentration be 1wt%, the concentration of Kynoar is 20wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, in the case where temperature is 30 DEG C and rotating speed is 150rpm
Isothermal reaction 72h, then the standing and defoaming 6h under conditions of temperature is 50 DEG C, obtain casting solution；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 1000 μm, then will contain thickness
The mould spent for 1000 μm of casting solution, which is placed in deionized water, stands 60min, obtains cured film；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, is dried under conditions of temperature is 50 DEG C
16h, obtains porous Kynoar composite Nano Ag films, the aperture of the film is 0.5-2.5 μm.
Example IV：A kind of preparation method of porous Kynoar composite Nano Ag films, comprises the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 20min under conditions of 1100rpm for 50 DEG C and mixing speed, obtains silver nitrate in suspension, the suspension
Concentration be 0.2wt%, the concentration of Kynoar is 7wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, temperature be 45 DEG C and rotating speed be under 70rpm it is permanent
Temperature reaction 48h, then the standing and defoaming 2h under conditions of temperature is 35 DEG C, obtain casting solution；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 300 μm, then will contain thickness
The mould spent for 300 μm of casting solution, which is placed in deionized water, stands 40min, obtains cured film；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, is dried under conditions of temperature is 35 DEG C
10h, obtains porous Kynoar composite Nano Ag films, the aperture of the film is 0.5-2.5 μm.
Embodiment five：A kind of preparation method of porous Kynoar composite Nano Ag films, comprises the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 40min under conditions of 1400rpm for 70 DEG C and mixing speed, obtains silver nitrate in suspension, the suspension
Concentration be 0.7wt%, the concentration of Kynoar is 15wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, in the case where temperature is 50 DEG C and rotating speed is 120rpm
Isothermal reaction 36h, then the standing and defoaming 4h under conditions of temperature is 45 DEG C, obtain casting solution；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 700 μm, then will contain thickness
The mould spent for 700 μm of casting solution, which is placed in deionized water, stands 50min, obtains cured film；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, is dried under conditions of temperature is 45 DEG C
14h, obtains porous Kynoar composite Nano Ag films, the aperture of the film is 0.5-2.5 μm.
Embodiment six：To examine the cell of the porous Kynoar composite Nano Ag films for artificial skin prepared
Activity, is tested as follows.
1）The sample as made from embodiment one is cut into the diaphragms of 1 × 1cm sizes, is soaked in 75% ethanol solution
30min is sterilized, is then rinsed 3 times with phosphate buffer；
2）By step 1）Diaphragm after processing adds the 1640 culture medium that 1mL contains 10% hyclone, in the bar that temperature is 37 DEG C
24h is extracted under part, leaching liquor is made；
3）Take the logarithm growth period human epidermal cell system HaCaT cells, 96 orifice plate cultures, 24 are seeded to by 3000/hole of density
Former culture medium is removed after hour cell adherent growth；
4）Experiment packet：
Experimental group：To step 3）The HaCaT cells of culture add step 2 by 100 μ L/ holes）Obtained leaching liquor；
Control group：To step 3）The HaCaT cells of culture add 1640 culture mediums containing 10% hyclone of equivalent；Experimental group
With the hole of the equal multiple cropping of control group 4；
Blank group：Without cell, 1640 culture mediums containing 10% hyclone are only added；
5）Culture three days, carries out cell propagation test experience, discards original leaching liquor or culture medium daily, slow with phosphate
Fliud flushing is washed twice to the cell of experimental group and control group respectively, and 100 μ L new culture medium and 10 μ L CCK8 are then added per hole
Reagent, the CCK8 reagents are purchased from the green skies company in Shanghai, after 2h is incubated under conditions of temperature is 37 DEG C, are existed with ELIASA
The absorbance per hole, the cytoactive of experiment with computing group are detected at 450nm wavelength, calculation formula is：（Experimental group absorbance-sky
White group absorbance）/ （Control group absorbance-blank group absorbance）× 100%, block diagram is drawn according to result of calculation.
As a result：Referring to Fig. 3, after inoculation first day, second day and the 3rd day, the cytoactive of experimental group was 95%
More than, the no significant difference compared with the cytoactive of control group points out the porous Kynoar composite Nano Ag films prepared
Without obvious cytotoxicity, security is higher.
Embodiment seven：To examine the porous Kynoar composite Nano Ag films for artificial skin prepared to resistance
The antibacterial activity of bacterium, is tested as follows：
1）Experiment packet：Experimental group：Porous Kynoar composite Nano Ag films, control group made from embodiment one：Concentration
For 1mg/mL streptomysin, concentration is 10 μM of tetracycline, and concentration is 10 μM of cefotaxime, blank group：It is added without any examination
Agent.
2）The sample as made from embodiment one is cut into the diaphragms of 1 × 1cm sizes, is soaked in 75% ethanol solution
30min is sterilized, is then rinsed 3 times with phosphate buffer；
3）Take the logarithm growth period Multi-drug resistant Acinetobacter baumannii, the Multi-drug resistant Acinetobacter baumannii is by Third Military Medical University
Southwestern hospital burn department microbial room is provided, and ELIASA is diluted to LB culture mediums and detects that absorbance is at 600nm wavelength
0.07 bacterium solution, the bacterium solution after dilution is added in 24 orifice plates by 500 μ L/ holes；
4）Step 2 is added into every pore fungi liquid of 24 orifice plates respectively）Obtained diaphragm, concentration for 1mg/mL streptomysin, dense
The cefotaxime that degree is 10 μM of tetracycline and concentration is 10 μM, then it is 50rpm with rotating speed that 24 orifice plates, which are placed in temperature to be 37 DEG C,
Shaking table in react 24h；
5）24 orifice plates are collected, 100 μ L bacterium solutions are drawn per hole into 96 orifice plates, absorbance is detected at 600nm wavelength with ELIASA
It is worth and records, draws block diagram.
As a result：Referring to Fig. 4, experimental group is substantially relatively low compared with the absorbance of blank group and control group, points out it significantly to press down
The growth of Acinetobacter bauamnnii processed, and effect is significantly better than antibiotic, therefore porous Kynoar composite Nano Ag films can
Effectively suppress the growth of drug-fast bacteria, with good antibacterial effect.
Embodiment eight：To examine the porous Kynoar composite Nano Ag films for artificial skin prepared to internal
The suppression of bacterium infection and the influence to wound healing, are tested as follows：
1) preparation of BALB/C mice (being purchased from Third Military Medical University's Experimental Animal Center) full thickness dermal wounds model：It is small
Mouse gives after 1% yellow Jackets intraperitoneal injection (100mg/kg, 0.01ml/g) anesthesia, is fixed on prone position and gives back art
Area's preserved skin.Art area then is sterilized with 75% ethanol cotton balls, the full thickness skin of an a diameter of 6mm is respectively made in back both sides with card punch
Wound Defect, is photographed to record to the surface of a wound immediately, and the surface of a wound area is used as initial surface of a wound area.
2) sample applies bacterium and prepared：Will porous Kynoar composite Nano Ag films and vaseline as made from embodiment one
Gauze is cut into 0.8 × 0.8cm sizes, and tow sides use ultraviolet disinfection 30min, takes 10 μ L to be diluted to 0.5 standard wheat
The Acinetobacter bauamnnii bacterium solution of family name's unit, is coated on standby after sample surface, natural air drying 10min.
3) experimental group：Porous Kynoar composite Nano Ag films are applied into bacterium face flap coverage, and use vacuum suction film
It is fixed；Control group：Petrolatum gauze is applied into bacterium face and is covered in the surface of a wound, and is fixed with vacuum suction film；Blank group：Only inhaled with negative pressure
Draw film and attach the surface of a wound.30 mouse are taken, 10 mouse is randomly assigned and is tested to every group.
4) it is postoperative to take 5 mouse opening surface of a wound to photograph to record respectively from three groups in the 3rd day, the 7th day, observe wound healing
Situation.Mouse after the 7th day using disconnected cervical approach in being put to death.Remaining 15 mouse (every group each remaining 5) removed solid at the 8th day
Fixed vacuum suction film, observes the surface of a wound daily, treats that complete epithelialization is that the surface of a wound heals completely to the surface of a wound, and the record surface of a wound is cured completely
The time required to closing.
5）The 3rd day and the 7th day each group surface of a wound photo are analyzed using the softwares of Image Pro Plus 6.0, measurement is surplus
Remaining surface of a wound area, i.e., the area that edge of wound is surrounded, and Wound healing rate is calculated as follows：
Wound healing rate=（Initial surface of a wound area-residue surface of a wound area）/ initial surface of a wound area × 100%；
6）Using one-way analysis of variance method respectively to needed for the 3rd day and the 7th day three groups of Wound healing rates and surface of a wound healing completely
Time carries out statistical analysis.
As a result：Porous Kynoar composite Nano Ag films flap coverage was showed no red and swollen and purulence at the 3rd day and the 7th day
Liquid oozes out, consistent with blank group, and the visible a large amount of festers of the petrolatum gauze group surface of a wound ooze out, and edge of wound is red and swollen.Referring to Fig. 5, when each
Between point Wound healing rate comparative result show that porous Kynoar composite Nano Ag films are obvious compared with petrolatum gauze group
Higher, there was no significant difference with the blank group without bacterium infection, points out porous Kynoar composite Nano Ag films to have
Effect prevents the invasion and attack of resistance Acinetobacter bauamnnii from infect, and the maintenance surface of a wound normally heals microenvironment.Referring to Fig. 6, during wound healing
Between comparative result show：Porous Kynoar composite Nano Ag films group substantially contracts compared with petrolatum gauze group healing time
It is short, point out porous Kynoar composite Nano Ag films to prevent harmful effect of the bacterium infection to wound healing.
Listed above is only the specific embodiment of the present invention.It is clear that the invention is not restricted to which above-described embodiment, can also have
Many operative combinations.It is all that one of ordinary skill in the art directly can export or associate from present disclosure
Situation, is considered to be protection scope of the present invention.
Claims (9)
1. a kind of preparation method of porous Kynoar composite Nano Ag films, it is characterised in that comprise the following steps：
1）Prepare suspension：By silver nitrate dispersing and dissolving in DMF, Kynoar is added, in temperature
It is magnetic agitation 10-60min under conditions of 1000-1500rpm for 40-80 DEG C and mixing speed, obtains suspension, the suspension
The concentration of silver nitrate is 0.1 ~ 1wt% in liquid, and the concentration of Kynoar is 5 ~ 20wt%；
2）Prepare casting solution：By step 1）Obtained suspension is placed in shaking table, temperature be 25-60 DEG C and rotating speed be 50-
12-72h, then 1-6h of standing and defoaming under conditions of temperature is 30-50 DEG C are reacted under conditions of 150rpm, casting solution is obtained；
3）Film forming：By step 2）Obtained casting solution is poured into mould, and the thickness for controlling casting solution is 100-1000 μm, then will
10-60min of standing in deionized water is placed in for the mould of 100-1000 μm of casting solution containing thickness, cured film is obtained；
4）Cleaning：By step 3）Obtained cured film is successively cleaned with ethanol and deionized water；
5）Dry：By step 4）Cured film after cleaning is placed in vacuum drying oven, is done under conditions of temperature is 30-50 DEG C
Dry 8-16h, obtains porous Kynoar composite Nano Ag films, the aperture of the film is 0.5-2.5 μm.
2. the preparation method of porous Kynoar composite Nano Ag films according to claim 1, it is characterised in that：Institute
State step 1）The concentration of silver nitrate is 0.425wt% in middle suspension, and the concentration of Kynoar is 10wt%.
3. the preparation method of porous Kynoar composite Nano Ag films according to claim 1 or 2, its feature exists
In：The step 1）In under conditions of temperature is 60 DEG C and mixing speed 1200rpm magnetic agitation 30min.
4. the preparation method of porous Kynoar composite Nano Ag films according to claim 1 or 2, its feature exists
In：The step 2）In be 37 DEG C in temperature and rotating speed is isothermal reaction 24h under conditions of 100rpm, in the bar that temperature is 40 DEG C
Standing and defoaming 1h under part.
5. the preparation method of porous Kynoar composite Nano Ag films according to claim 1 or 2, its feature exists
In：The step 3）The material of middle mould is glass, polytetrafluoroethylene (PTFE) or polyester.
6. the preparation method of porous Kynoar composite Nano Ag films according to claim 1 or 2, its feature exists
In：The step 3）The middle thickness for controlling casting solution is 500 μm, then the mould containing thickness for 500 μm of casting solution is placed in
30min is stood in deionized water, cured film is obtained.
7. the preparation method of porous Kynoar composite Nano Ag films according to claim 1 or 2, its feature exists
In：The step 5）In temperature be 40 DEG C under conditions of dry 12h.
8. it is prepared by the porous Kynoar composite Nano Ag films as made from the method described in claim 1-7 any bar
For the purposes in the artificial skin or surgical dressing of the defect of skin for the treatment of people.
9. purposes according to claim 8, the defect of skin is skin full-thickness defects or the skin infection surface of a wound.
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