CN107324506A - A kind of preparation method for handling breeding wastewater biological agent - Google Patents
A kind of preparation method for handling breeding wastewater biological agent Download PDFInfo
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- CN107324506A CN107324506A CN201710510695.1A CN201710510695A CN107324506A CN 107324506 A CN107324506 A CN 107324506A CN 201710510695 A CN201710510695 A CN 201710510695A CN 107324506 A CN107324506 A CN 107324506A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The present invention relates to a kind of preparation method for handling breeding wastewater biological agent, belong to technical field of waste water processing.Present invention luffa is used as template, load calcium hydroxy phosphate, final high temperature calcining removes luffa template, so as to obtain with high porosity, the calcium hydroxy phosphate carrier of high power capacity, solve the problem of conventional carriers capacity is low, and calcium hydroxy phosphate has good biocompatibility, the problem of solving the poor compatibility of conventional carriers and microorganism again, sticky rice syrup is sufficiently submerged in carrier inside again, sticky rice syrup is set to be transformed into sticky spawn under thermodynamic activity, last and bacterium bacteria suspension mixed fermentation, function bacterium is set to be fixed on carrier, immobilization biological preparation is made, glutinous rice flour generation thick gelatinous mass can for function bacterium provide survive needed for nutrient, so as to extend effective acting time and the service life of immobilization biological preparation, have broad application prospects.
Description
Technical field
The present invention relates to a kind of preparation method for handling breeding wastewater biological agent, belong to technical field of waste water processing.
Background technology
Substantial amounts of artificial bait throwing in, Metabolite Accumulation cause water quality deterioration, are that current batch production cyclic culture pattern is present
Subject matter.Ammonia nitrogen, nitrite nitrogen that residual feed and excreta and organic debris etc. discharge after being acted on through microorganisms in water etc. are harmful to
Material, easily causes cultivated animals poisoning, or even dead, and the direct discharge of breeding wastewater is easily caused the eutrophy of water proximate
Change, therefore the existence form and concentration of control water body nitrogen are the key links of cultivating wastewater purification.
In aquatic ecosystem, the ammonification that is participated in by microorganism, nitrification and denitrification act on system Nitrogen Cycling, defeated
Played an important role in going out.Because free bacterial growth rate is relatively low, sensitive to low temperature environment, system nitrification, denitrifying effect
Rate is typically relatively low.The erosion resistibility of function bacterium can be effectively improved by immobilized microorganism technology and journey is stood to low temperature
Degree etc., so as to effectively improve removal effect of the function bacterium to harmful nitrogen such as breeding water body ammonia nitrogen, nitrite nitrogen.But it is individually solid
Surely microbe treatment system is changed due to the poor compatibility between function bacterium and carrier, the capacity of carrier is low to cause antijamming capability weak,
The biomass and activity decrease of the poor reactive volt-ampere hour functional microorganism of environmental nutrient, effective acting time are short.
Therefore, the biological agent of a kind of high power capacity, high-affinity and long action time is invented to cultivating wastewater purification skill
Art field has positive meaning.
The content of the invention
Present invention mainly solves technical problem, at present with treatment based on immobilized microorganisms breeding wastewater when, due to work(
Poor compatibility between energy bacterium and carrier, the capacity of carrier is low to cause antijamming capability weak, the poor reactive volt-ampere hour functional microorganism of environmental nutrient
Biomass and activity decrease, there is provided a kind of preparation for handling breeding wastewater biological agent for effective acting time short defect
Method.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
（1）Take Fresh Lemon to squeeze the juice, obtain lemon juice, then take luffa, after being rinsed with water, luffa and lemon juice are mixed simultaneously
Sonic oscillation is impregnated, and dipping is filtered after terminating, isolated dipping luffa；
（2）Above-mentioned dipping luffa is added in calcium chloride solution, shaking table vibration dipping obtains mixed liquor, adjusted with ammoniacal liquor
PH of mixed obtains precursor liquid to 9.0~9.3；
（3）Phosphorus pentoxide and absolute ethyl alcohol are mixed into reaction, titrating solution is obtained, stirs precursor liquid with agitator, stirring
Mix in titrating solution instillation precursor liquid under state, after completion of dropping, reduce speed of agitator, continue stirring reaction；
（4）It is still aging after above-mentioned reaction terminates, filter cake is filtrated to get, then filter cake is calcined, calcining terminates rear furnace cooling
To room temperature, discharging produces self-control fixation support, standby；
（5）Ground after glutinous rice and water are mixed with stone mill, collect the milky slurries that grinding is obtained, carried above-mentioned from preparing curable
Body is added in milky slurries, and pretreatment fluid is obtained after being uniformly mixed, and pretreatment fluid is concentrated into pretreatment fluid substance
Long-pending 1/5, obtains concentrate；
（6）Above-mentioned concentrate is put into steam box, steamed, gelatinous mixture is taken out after steaming end, crushes, obtains biological system
Agent presoma；
（7）It is fitted into after above-mentioned biological agent presoma and bacterium bacteria suspension are mixed in fermentation tank, sealing and fermenting, after fermentation ends
Tunning is taken out, is separated by filtration and obtains the filter cake that ferments, will be crushed after fermentation filter cake vacuum freeze-drying, you can obtain processing cultivation useless
Aquatic organism preparation.
Described bacterium bacteria suspension is one or more mixing in nitrobacteria, denitrifying bacteria and amonifying bacteria
Thing, is matched in any proportion during mixing.
Step（3）Described in phosphorus pentoxide and absolute ethyl alcohol mass ratio be 1:8, stir precursor liquid with agitator
Rotating speed is 200~300r/min, and the speed of agitator after reduction is 50~60r/min, and the stirring reaction time is 30~40min.
Step（4）Described in digestion time be 12~14h, calcining heat be 300~400 DEG C, calcination time be 2~
4h。
Step（5）Described in glutinous rice and water mass ratio be 1:5, make the quality of fixation support and milky slurries by oneself
Than for 1:5.
Step（6）Described in steaming temperature be 100~105 DEG C, steaming time be 3~5min, grinding time be 20~
30min。
Step（7）Described in bacterium bacteria suspension concentration be 105Cfu/mL, biological agent presoma and bacterium bacteria suspension
Mass ratio is 1:2, sealing and fermenting temperature is 35~45 DEG C, and the heat-preservation fermentation time is 3~5 days.
The beneficial effects of the invention are as follows：
（1）The present invention, as template, is impregnated luffa with lemon juice first, makes luffa surface with the luffa of porous high power capacity
Adhere to organic carboxyl acid, using the sequestering of organic carboxyl acid, the calcium ion chelating in calcium chloride solution be fixed on luffa surface,
Then by phosphorus pentoxide and alcohol reaction generation phosphatide, under ammoniacal liquor effect, phosphatide is gradually hydrolyzed, on phospholipid hydrolysis product
The calcium ion of hydroxyl and template surface is slowly combined, and is produced nucleus, is increased gradually grow up over time, finally give surface and carry
The luffa of calcium hydroxy phosphate, final high temperature calcining removes luffa template, so as to obtain the hydroxyl with high porosity, high power capacity
Base calcium phosphate carrier, solves the problem of conventional carriers capacity is low, and calcium hydroxy phosphate has good biocompatibility, solves again
The problem of conventional carriers of having determined and the poor compatibility of microorganism；
（2）The present invention by glutinous rice by grinding to form slurry, then by sticky rice syrup and makes carrier hybrid infusion by oneself, is sufficiently submerged in sticky rice syrup
Carrier inside, then sticky rice syrup is transformed into sticky spawn under thermodynamic activity, last and bacterium bacteria suspension mixing hair
Ferment, makes function bacterium be fixed on carrier, wherein function bacterium can be firmly fixed in sticky spawn, plays sustained release and makees
With, and the thick gelatinous mass generated by glutinous rice flour can also provide nutrient needed for surviving for function bacterium, so as to extend solid
Surely effective acting time and the service life of biological agent are changed, in the biomass and activity of the poor reactive volt-ampere hour functional microorganism of environmental nutrient
Higher level can be still kept, is had broad application prospects.
Embodiment
Take Fresh Lemon to squeeze the juice, obtain lemon juice, then take luffa, be in mass ratio 1 after being rinsed 3~5 times with water:3 will
Sonic oscillation impregnates 30~40min after luffa and lemon juice mixing, and dipping is filtered after terminating, isolated dipping luffa；
It is 1 in mass ratio by dipping luffa:10 are added to mass fraction in 40% calcium chloride solution, shaking table vibration impregnates 1~2h,
Mixed liquor is obtained, is that 20% ammoniacal liquor adjusts pH of mixed to 9.0~9.3 with mass fraction, obtains precursor liquid；It is 1 in mass ratio：8
Phosphorus pentoxide and absolute ethyl alcohol are mixed into 1~2h of reaction, titrating solution is obtained, is turned with agitator with 200~300r/min
Speed stirring precursor liquid, instills titrating solution in precursor liquid under stirring, after completion of dropping, and reduction speed of agitator to 50~
60r/min, continues 30~40min of stirring reaction；After above-mentioned reaction terminates, still aging 12~14h is filtrated to get filter cake,
Filter cake is put into Muffle furnace again, 2~4h is calcined at 300~400 DEG C, calcining cools to room temperature with the furnace after terminating, and discharges, i.e.,
Derived from preparing curable carrier；It is 1 in mass ratio by glutinous rice and water:Ground 3~5 times with stone mill after 5 mixing, collect what grinding was obtained
Milky slurries, are 1 in mass ratio by above-mentioned self-control fixation support:5 are added in milky slurries, after being uniformly mixed
Pretreatment fluid is obtained, pretreatment fluid is put into concentration tank, the 1/5 of pretreatment fluid original volume is concentrated into, obtains concentrate；Will be upper
State concentrate to pour into stainless steel pallet, then stainless steel pallet be put into steam box, 3~5min is steamed at 100~105 DEG C,
Gelatinous mixture is taken out after steaming end, 20~30min of crushing in airslide disintegrating mill is put into, obtains biological agent presoma；
It is 10 by above-mentioned biological agent presoma and concentration5Cfu/mL bacterium bacteria suspension is 1 in mass ratio:Load fermentation after 2 mixing
In tank, sealed fermenter, heat-preservation fermentation 3~5 days, take out tunning at 35~45 DEG C after fermentation ends, are separated by filtration
To fermentation filter cake, it will be crushed after fermentation filter cake vacuum freeze-drying, you can obtain handling breeding wastewater biological agent.Described bacterium bacterium
Suspension is one or more mixtures in nitrobacteria, denitrifying bacteria and amonifying bacteria, is matched somebody with somebody in any proportion during mixing
Than.
Example 1
Take Fresh Lemon to squeeze the juice, obtain lemon juice, then take luffa, be in mass ratio 1 after being rinsed 3 times with water:3 by luffa
With sonic oscillation dipping 30min after lemon juice mixing, dipping is filtered after terminating, isolated dipping luffa；Sponge gourd will be impregnated
Network is 1 in mass ratio:10 are added to mass fraction in 40% calcium chloride solution, shaking table vibration dipping 1h obtains mixed liquor, used
Mass fraction is that 20% ammoniacal liquor adjusts pH of mixed to 9.0, obtains precursor liquid；It is 1 in mass ratio：8 by phosphorus pentoxide and anhydrous
Ethanol mixes reaction 1h, obtains titrating solution, and precursor liquid is stirred with 200r/min rotating speeds with agitator, will under stirring
Titrating solution is instilled in precursor liquid, after completion of dropping, reduction speed of agitator to 50r/min, continues stirring reaction 30min；Treat above-mentioned
After reaction terminates, still aging 12h is filtrated to get filter cake, then filter cake is put into Muffle furnace, and 2h is calcined at 300 DEG C, calcining
Cool to room temperature after end with the furnace, discharge, produce self-control fixation support；It is 1 in mass ratio by glutinous rice and water:Used after 5 mixing
Stone mill is ground 3 times, collects the milky slurries that grinding is obtained, and is 1 in mass ratio by above-mentioned self-control fixation support:5 are added to
In milky slurries, pretreatment fluid is obtained after being uniformly mixed, pretreatment fluid is put into concentration tank, pretreatment fluid is concentrated into
The 1/5 of original volume, obtains concentrate；Above-mentioned concentrate is poured into stainless steel pallet, then stainless steel pallet is put into steam box,
3min is steamed at 100 DEG C, gelatinous mixture is taken out after steaming end, is put into airslide disintegrating mill and crushes 20min, given birth to
Thing preparation presoma；It is 10 by above-mentioned biological agent presoma and concentration5Cfu/mL bacterium bacteria suspension is 1 in mass ratio:2 mix
It is fitted into after conjunction in fermentation tank, sealed fermenter, heat-preservation fermentation 3 days at 35 DEG C, tunning, filtering point is taken out after fermentation ends
From the filter cake that ferments is obtained, it will be crushed after fermentation filter cake vacuum freeze-drying, you can obtain handling breeding wastewater biological agent.Described is thin
Bacterium bacteria suspension is one or more mixtures in nitrobacteria, denitrifying bacteria and amonifying bacteria, and any ratio is pressed during mixing
Example proportioning.
Example 2
Take Fresh Lemon to squeeze the juice, obtain lemon juice, then take luffa, be in mass ratio 1 after being rinsed 4 times with water:3 by luffa
With sonic oscillation dipping 35min after lemon juice mixing, dipping is filtered after terminating, isolated dipping luffa；Sponge gourd will be impregnated
Network is 1 in mass ratio:10 are added to mass fraction in 40% calcium chloride solution, shaking table vibration dipping 1h obtains mixed liquor, used
Mass fraction is that 20% ammoniacal liquor adjusts pH of mixed to 9.2, obtains precursor liquid；It is 1 in mass ratio：8 by phosphorus pentoxide and anhydrous
Ethanol mixes reaction 1h, obtains titrating solution, and precursor liquid is stirred with 250r/min rotating speeds with agitator, will under stirring
Titrating solution is instilled in precursor liquid, after completion of dropping, reduction speed of agitator to 55r/min, continues stirring reaction 35min；Treat above-mentioned
After reaction terminates, still aging 13h is filtrated to get filter cake, then filter cake is put into Muffle furnace, and 3h is calcined at 350 DEG C, calcining
Cool to room temperature after end with the furnace, discharge, produce self-control fixation support；It is 1 in mass ratio by glutinous rice and water:Used after 5 mixing
Stone mill is ground 4 times, collects the milky slurries that grinding is obtained, and is 1 in mass ratio by above-mentioned self-control fixation support:5 are added to
In milky slurries, pretreatment fluid is obtained after being uniformly mixed, pretreatment fluid is put into concentration tank, pretreatment fluid is concentrated into
The 1/5 of original volume, obtains concentrate；Above-mentioned concentrate is poured into stainless steel pallet, then stainless steel pallet is put into steam box,
4min is steamed at 103 DEG C, gelatinous mixture is taken out after steaming end, is put into airslide disintegrating mill and crushes 25min, given birth to
Thing preparation presoma；It is 10 by above-mentioned biological agent presoma and concentration5Cfu/mL bacterium bacteria suspension is 1 in mass ratio:2 mix
It is fitted into after conjunction in fermentation tank, sealed fermenter, heat-preservation fermentation 4 days at 40 DEG C, tunning, filtering point is taken out after fermentation ends
From the filter cake that ferments is obtained, it will be crushed after fermentation filter cake vacuum freeze-drying, you can obtain handling breeding wastewater biological agent.Described is thin
Bacterium bacteria suspension is one or more mixtures in nitrobacteria, denitrifying bacteria and amonifying bacteria, and any ratio is pressed during mixing
Example proportioning.
Example 3
Take Fresh Lemon to squeeze the juice, obtain lemon juice, then take luffa, be in mass ratio 1 after being rinsed 5 times with water:3 by luffa
With sonic oscillation dipping 40min after lemon juice mixing, dipping is filtered after terminating, isolated dipping luffa；Sponge gourd will be impregnated
Network is 1 in mass ratio:10 are added to mass fraction in 40% calcium chloride solution, shaking table vibration dipping 2h obtains mixed liquor, used
Mass fraction is that 20% ammoniacal liquor adjusts pH of mixed to 9.3, obtains precursor liquid；It is 1 in mass ratio：8 by phosphorus pentoxide and anhydrous
Ethanol mixes reaction 2h, obtains titrating solution, and precursor liquid is stirred with 300r/min rotating speeds with agitator, will under stirring
Titrating solution is instilled in precursor liquid, after completion of dropping, reduction speed of agitator to 60r/min, continues stirring reaction 40min；Treat above-mentioned
After reaction terminates, still aging 14h is filtrated to get filter cake, then filter cake is put into Muffle furnace, and 4h is calcined at 400 DEG C, calcining
Cool to room temperature after end with the furnace, discharge, produce self-control fixation support；It is 1 in mass ratio by glutinous rice and water:Used after 5 mixing
Stone mill is ground 5 times, collects the milky slurries that grinding is obtained, and is 1 in mass ratio by above-mentioned self-control fixation support:5 are added to
In milky slurries, pretreatment fluid is obtained after being uniformly mixed, pretreatment fluid is put into concentration tank, pretreatment fluid is concentrated into
The 1/5 of original volume, obtains concentrate；Above-mentioned concentrate is poured into stainless steel pallet, then stainless steel pallet is put into steam box,
5min is steamed at 105 DEG C, gelatinous mixture is taken out after steaming end, is put into airslide disintegrating mill and crushes 30min, given birth to
Thing preparation presoma；It is 10 by above-mentioned biological agent presoma and concentration5Cfu/mL bacterium bacteria suspension is 1 in mass ratio:2 mix
It is fitted into after conjunction in fermentation tank, sealed fermenter, heat-preservation fermentation 5 days at 45 DEG C, tunning, filtering point is taken out after fermentation ends
From the filter cake that ferments is obtained, it will be crushed after fermentation filter cake vacuum freeze-drying, you can obtain handling breeding wastewater biological agent.Described is thin
Bacterium bacteria suspension is one or more mixtures in nitrobacteria, denitrifying bacteria and amonifying bacteria, and any ratio is pressed during mixing
Example proportioning.
Comparative example
Fixation is made in the one or more mixtures fixed with unloaded haydite in nitrobacteria, denitrifying bacteria and amonifying bacteria
Change microbial inoculum as a comparison case.
Processing breeding wastewater biological agent produced by the present invention and commercially available SHH-102 ammonia nitrogen removal microbial inoculums are respectively used to
Handle breeding wastewater,（Breeding wastewater COD concentration is 30.5mg/L, and total nitrogen concentration is 14.3mg/L, and nitrite nitrogen concentration is
0.023mg/L, nitrate is 6.8mg/L, and ammonia nitrogen concentration is 0.87mg/L）The waste water after processing is handled after 10 days
Effect detection, testing result such as table 1：
Table 1
| Detection project | Example 1 | Example 2 | Example 3 | Comparative example |
| Effective acting time（My god） | 25 | 27 | 30 | 10 |
| Turbidity（NTU） | 1.8 | 1.6 | 1.4 | 5.0 |
| Total nitrogen（mg/L） | 3.0 | 3.1 | 2.8 | 10.2 |
| Nitrite nitrogen（mg/L） | 0.013 | 0.011 | 0.010 | 0.016 |
| Nitrate nitrogen（mg/L） | 1.11 | 1.12 | 1.10 | 2.30 |
| Ammonia nitrogen（mg/L） | 0.35 | 0.32 | 0.34 | 0.45 |
| COD（mg/L） | 24.5 | 23.8 | 23.7 | 28.0 |
| Effective bacterial number（lgMPN/mL） | 5.5 | 5.4 | 5.2 | 3.5 |
The data in table 1, the present invention is significantly improved compared to comparative example to the treatment effect of breeding wastewater, effective acting time
It is extended, the biomass and activity of microorganism can be maintained for a long time, be had broad application prospects.
Claims (7)
1. a kind of preparation method for handling breeding wastewater biological agent, it is characterised in that specifically preparation process is：
（1）Take Fresh Lemon to squeeze the juice, obtain lemon juice, then take luffa, after being rinsed with water, luffa and lemon juice are mixed simultaneously
Sonic oscillation is impregnated, and dipping is filtered after terminating, isolated dipping luffa；
（2）Above-mentioned dipping luffa is added in calcium chloride solution, shaking table vibration dipping obtains mixed liquor, adjusted with ammoniacal liquor
PH of mixed obtains precursor liquid to 9.0~9.3；
（3）Phosphorus pentoxide and absolute ethyl alcohol are mixed into reaction, titrating solution is obtained, stirs precursor liquid with agitator, stirring
Mix in titrating solution instillation precursor liquid under state, after completion of dropping, reduce speed of agitator, continue stirring reaction；
（4）It is still aging after above-mentioned reaction terminates, filter cake is filtrated to get, then filter cake is calcined, calcining terminates rear furnace cooling
To room temperature, discharging produces self-control fixation support, standby；
（5）Ground after glutinous rice and water are mixed with stone mill, collect the milky slurries that grinding is obtained, carried above-mentioned from preparing curable
Body is added in milky slurries, and pretreatment fluid is obtained after being uniformly mixed, and pretreatment fluid is concentrated into pretreatment fluid substance
Long-pending 1/5, obtains concentrate；
（6）Above-mentioned concentrate is put into steam box, steamed, gelatinous mixture is taken out after steaming end, crushes, obtains biological system
Agent presoma；
（7）It is fitted into after above-mentioned biological agent presoma and bacterium bacteria suspension are mixed in fermentation tank, sealing and fermenting, after fermentation ends
Tunning is taken out, is separated by filtration and obtains the filter cake that ferments, will be crushed after fermentation filter cake vacuum freeze-drying, you can obtain processing cultivation useless
Aquatic organism preparation.
2. a kind of preparation method for handling breeding wastewater biological agent according to claim 1, it is characterised in that：Described
Bacterium bacteria suspension is one or more mixtures in nitrobacteria, denitrifying bacteria and amonifying bacteria, by any during mixing
Proportions.
3. a kind of preparation method for handling breeding wastewater biological agent according to claim 1, it is characterised in that：Step
（3）Described in phosphorus pentoxide and absolute ethyl alcohol mass ratio be 1:8, with agitator stir the rotating speed of precursor liquid for 200~
300r/min, the speed of agitator after reduction is 50~60r/min, and the stirring reaction time is 30~40min.
4. a kind of preparation method for handling breeding wastewater biological agent according to claim 1, it is characterised in that：Step
（4）Described in digestion time be 12~14h, calcining heat be 300~400 DEG C, calcination time be 2~4h.
5. a kind of preparation method for handling breeding wastewater biological agent according to claim 1, it is characterised in that：Step
（5）Described in glutinous rice and water mass ratio be 1:5, the mass ratio for making fixation support and milky slurries by oneself is 1:5.
6. a kind of preparation method for handling breeding wastewater biological agent according to claim 1, it is characterised in that：Step
（6）Described in steaming temperature be 100~105 DEG C, steaming time be 3~5min, grinding time be 20~30min.
7. a kind of preparation method for handling breeding wastewater biological agent according to claim 1, it is characterised in that：Step
（7）Described in bacterium bacteria suspension concentration be 105The mass ratio of cfu/mL, biological agent presoma and bacterium bacteria suspension is 1:2,
Sealing and fermenting temperature is 35~45 DEG C, and the heat-preservation fermentation time is 3~5 days.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109896619A (en) * | 2018-04-04 | 2019-06-18 | 徐雄剑 | A kind of preparation method of the filler for fluid bed biological film method |
| CN110918114A (en) * | 2019-11-25 | 2020-03-27 | 芜湖职业技术学院 | Wine-making waste liquid decoloring agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1369553A (en) * | 2002-03-22 | 2002-09-18 | 陕西杨凌瑞智德生物技术研究所 | Process for preparing solid active lactobacillus preparation |
| CN103214151A (en) * | 2013-04-27 | 2013-07-24 | 安徽蓝田特种龟鳖有限公司 | Method for ecologically and compensatively treating cultivation wastewater in testudinate greenhouse |
| CN105331556A (en) * | 2015-11-12 | 2016-02-17 | 河南兴禾生物科技有限公司 | Composite micro-ecological preparation and preparation method and application thereof |
-
2017
- 2017-06-28 CN CN201710510695.1A patent/CN107324506A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1369553A (en) * | 2002-03-22 | 2002-09-18 | 陕西杨凌瑞智德生物技术研究所 | Process for preparing solid active lactobacillus preparation |
| CN103214151A (en) * | 2013-04-27 | 2013-07-24 | 安徽蓝田特种龟鳖有限公司 | Method for ecologically and compensatively treating cultivation wastewater in testudinate greenhouse |
| CN105331556A (en) * | 2015-11-12 | 2016-02-17 | 河南兴禾生物科技有限公司 | Composite micro-ecological preparation and preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109896619A (en) * | 2018-04-04 | 2019-06-18 | 徐雄剑 | A kind of preparation method of the filler for fluid bed biological film method |
| CN110918114A (en) * | 2019-11-25 | 2020-03-27 | 芜湖职业技术学院 | Wine-making waste liquid decoloring agent and preparation method thereof |
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Application publication date: 20171107 |