CN107319564A - Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared - Google Patents
Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared Download PDFInfo
- Publication number
- CN107319564A CN107319564A CN201710542494.XA CN201710542494A CN107319564A CN 107319564 A CN107319564 A CN 107319564A CN 201710542494 A CN201710542494 A CN 201710542494A CN 107319564 A CN107319564 A CN 107319564A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- colon
- mouse
- colitis
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 64
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 64
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 63
- 206010009900 Colitis ulcerative Diseases 0.000 title claims abstract description 16
- 201000006704 Ulcerative Colitis Diseases 0.000 title claims abstract description 16
- 239000003814 drug Substances 0.000 title claims abstract description 11
- 235000013305 food Nutrition 0.000 title claims abstract description 11
- 230000002265 prevention Effects 0.000 title claims abstract description 9
- 230000036541 health Effects 0.000 abstract description 2
- 210000001072 colon Anatomy 0.000 description 63
- 241000699666 Mus <mouse, genus> Species 0.000 description 54
- 108020004999 messenger RNA Proteins 0.000 description 33
- 206010009887 colitis Diseases 0.000 description 32
- 238000003757 reverse transcription PCR Methods 0.000 description 20
- 230000003321 amplification Effects 0.000 description 19
- 238000003199 nucleic acid amplification method Methods 0.000 description 19
- 210000001519 tissue Anatomy 0.000 description 18
- 241000699670 Mus sp. Species 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 108090001007 Interleukin-8 Proteins 0.000 description 12
- 102000004890 Interleukin-8 Human genes 0.000 description 12
- 102100020880 Kit ligand Human genes 0.000 description 11
- 101710177504 Kit ligand Proteins 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 10
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 241000193830 Bacillus <bacterium> Species 0.000 description 9
- 108010002350 Interleukin-2 Proteins 0.000 description 9
- 102000016971 Proto-Oncogene Proteins c-kit Human genes 0.000 description 9
- 108010014608 Proto-Oncogene Proteins c-kit Proteins 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 230000000692 anti-sense effect Effects 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 235000013618 yogurt Nutrition 0.000 description 7
- 102000002791 Interleukin-8B Receptors Human genes 0.000 description 6
- 108010018951 Interleukin-8B Receptors Proteins 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 206010061218 Inflammation Diseases 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 230000004054 inflammatory process Effects 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 102000016938 Catalase Human genes 0.000 description 4
- 108010053835 Catalase Proteins 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 210000004495 interstitial cells of cajal Anatomy 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000012764 semi-quantitative analysis Methods 0.000 description 4
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000003304 gavage Methods 0.000 description 3
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 3
- 208000035861 hematochezia Diseases 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000003449 preventive effect Effects 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000011725 BALB/c mouse Methods 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 101000998969 Homo sapiens Inositol-3-phosphate synthase 1 Proteins 0.000 description 2
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 2
- 102100036881 Inositol-3-phosphate synthase 1 Human genes 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- VLQGDKKHHCKIOJ-UHFFFAOYSA-N NNOS Chemical compound NNOS VLQGDKKHHCKIOJ-UHFFFAOYSA-N 0.000 description 2
- 240000005373 Panax quinquefolius Species 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010876 biochemical test Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000000112 colonic effect Effects 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000009266 disease activity Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- SJHPCNCNNSSLPL-CSKARUKUSA-N (4e)-4-(ethoxymethylidene)-2-phenyl-1,3-oxazol-5-one Chemical compound O1C(=O)C(=C/OCC)\N=C1C1=CC=CC=C1 SJHPCNCNNSSLPL-CSKARUKUSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- -1 GelRed nucleic acid Chemical class 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 208000019430 Motor disease Diseases 0.000 description 1
- 102000003896 Myeloperoxidases Human genes 0.000 description 1
- 108090000235 Myeloperoxidases Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 241000594182 Sarcophaga sigma Species 0.000 description 1
- 210000000447 Th1 cell Anatomy 0.000 description 1
- 210000004241 Th2 cell Anatomy 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000035605 chemotaxis Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 210000003038 endothelium Anatomy 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 230000002962 histologic effect Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009654 indole test Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 210000004493 neutrocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000003617 peroxidasic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000013094 purity test Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 230000019086 sulfide ion homeostasis Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
It is CCTCC NO the invention discloses deposit number:Applications of the M2016749 Lactobacillus plantarum YS3 (Lactobacillus plantarum YS3) in the food or medicine of prevention ulcerative colitis is prepared, not only expand Lactobacillus plantarum YS3 application, its application value is improved, and new hope is brought to the treatment or health care of ulcerative colitis.
Description
Technical field
The invention belongs to technical field of microbe application, it is related to a kind of Lactobacillus plantarum answering in food or medicine is prepared
With.
Background technology
Yak yogurt is a kind of spontaneous fermentation dairy products in Qinghai-Tibet Tibetan area, contains abundant nutritional ingredient, tool
There are anti-oxidant, reduction cholesterol, regulation immunity.Due to special spontaneous fermentation condition, including fermentation raw material breast, hair
Ferment temperature, fermentation time, fermentation vessel, particularly special fermentative microorganism composition, cause yak yogurt to have flavour
And quality.
Human ulcerative colitis is a kind of IBD of 2 type helper T lymphocyte (Th2) mediation, its cause of disease and hair
Interpretation of the cause, onset and process of an illness system is unclear, and therapeutic advance is slow and lacks specificity.The colitis of azolactone induction is a kind of Th2 of IL-4 mediations
Type inflammation, its histologic characteristics and inflammation are distributed similar human ulcerative colitis, the mouse Colon induced by Ci , azolactones
Scorching model can study and assess curative effect of medication or functional food physiological activity as Ulcerative Colitis
Beneficial instrument.
The content of the invention
It is an object of the invention to the mouse colitis model of Tong Guo azolactones induction, investigation is separated from yak yogurt
The Lactobacillus plantarum YS3 arrived is to the preventive effect of ulcerative colitis, to research and develop the food that can prevent ulcerative colitis
Or medicine.
Through research, the present invention provides following technical scheme:
Deposit number is CCTCC NO:M2016749 Lactobacillus plantarum YS3 (Lactobacillus plantarum
YS3) the application in the food or medicine of prevention ulcerative colitis is prepared.
Lactobacillus plantarum YS3 (Lactobacillus plantarum YS3) is the yak from Yushu district, Qinghai Tibetan autonomous prefecture
It is isolated in ox yogurt, it is preserved in China typical culture collection center (abbreviation CCTCC, address on December 14th, 2016:
No. 299 Wuhan Universitys of Wuhan City, Hubei Province Wuchang District Bayi Road), deposit number is CCTCC NO:M2016749.
After Shi azolactones induction BALB/c mouse colitis of the present invention, by determining, serum is related in colon to be referred to
Mark detection Lactobacillus plantarum YS3 colitis preventive effect.Experimental result shows that Lactobacillus plantarum YS3 can significantly reduce colon
The disease activity index (Disease activity index, DAI) of scorching mouse, suppresses the colon lengths contracting that colitis is caused
It is short, improve the ratio of colon weight/colon lengths;Colitis mice colon myeloperoxidase can be significantly reduced
(MPO), NO, MDA content and raising GSH contents;IL-2 levels and reduction IL- in colitis mice serum can also be significantly improved
10 levels.RT-PCR experimental results show that Lactobacillus plantarum YS3 can significantly raise neural in colitis mice colon
Type NOS (nNOS), endothelium in type NOS (eNOS), c-Kit, SCF mRNA expression, downward induce type NOS (iNOS), IL-8,
CXCR2 mRNA are expressed.As can be seen here, the mouse colitis that Lactobacillus plantarum YS3 is induced azolactone has good pre- preventive effect
Really, available for the food or medicine for preparing prevention ulcerative colitis.
The beneficial effects of the present invention are:Prevention ulcerative colitis is being prepared the invention provides Lactobacillus plantarum YS3
Food or medicine in application, not only expand Lactobacillus plantarum YS3 application, improve its application value, and
Treatment or health care to ulcerative colitis bring new hope.
Brief description of the drawings
Fig. 1 is the agarose gel electrophoresis figure of Lactobacillus plantarum YS3 genomic DNA, and wherein M is λ DNA/Hind III
Marker, 1 is Lactobacillus plantarum YS3 genomic DNA.
Fig. 2 is the PCR amplifications of Lactobacillus plantarum YS3 16S rDNA genetic fragments, and wherein M is DNA Marker,
C is negative control, and 1 is the pcr amplification product of Lactobacillus plantarum YS3 16S rDNA genetic fragments.
Fig. 3 is Lactobacillus plantarum YS3 Dui azolactone inducing colitis mouse Colon tissue nNOS, eNOS and iNOS mRNA
The influence of expression.
Fig. 4 is what Lactobacillus plantarum YS3 Dui azolactone inducing colitis mouse Colon tissue c-Kit and SCF mRNA were expressed
Influence.
Fig. 5 is what Lactobacillus plantarum YS3 Dui azolactone inducing colitis mouse Colon tissues IL-8 and CXCR2mRNA were expressed
Influence.
Fig. 3 is into Fig. 5, and LB represents lactobacillus bulgaricus, and LP-YS3 represents Lactobacillus plantarum YS3.
Embodiment
In order that the object, technical solutions and advantages of the present invention are clearer, below in conjunction with accompanying drawing to the excellent of the present invention
Embodiment is selected to be described in detail.
The separation and identification of embodiment 1, Lactobacillus plantarum YS3
First, lactic acid bacteria isolates and purifies
Gather 10 parts of the spontaneous fermentation yak yogurt sample of Yushu district, Qinghai Tibetan autonomous prefecture herdsman family, every part of sample nothing
After bacterium glass rod is fully stirred evenly, draw 50mL and be put into sterile centrifugation tube, be placed in food sampling box and take back laboratory, 4 DEG C of refrigerators
Preserve.
Every part of yak yogurt sample takes 1mL, and 10 times of gradient dilutions are made to 10 with sterile saline-7.Take 10-5、10-6、
10-7Each 1mL dilutions of three dilution factors respectively at equipped with 15mL MRS culture mediums (containing mass ratio be 5% CaCO3) plate
In, mix, put 30 DEG C of 72 ± 3h of insulating box culture.Each three, dilution factor work is parallel.After bacterium colony is formed, its form is observed special
Levy, the different bacterium colonies that picking has molten calcium circle are inoculated in degreasing milk medium respectively, put 30 DEG C of 24~48h of culture, treat that bacterial strain is given birth to
After length is good, streak inoculation puts 30 DEG C of culture 48h in MRS agar mediums;Above step is repeated at least three times, until obtaining
Pure bacterium colony.
Pure bacterial strain is rule on MRS solid mediums, 30 DEG C of culture 48h are put, with 10 times of amplification sem observation bacterium colony sizes,
The appearance features such as shape, carry out Gram's staining, and the typical bacterial strain of picking form carries out catalase test.Gram is contaminated
Color is positive, catalase test is negative meningitidis strains and bacillus strain, are fixed tentatively as lactic acid bacteria.
2nd, the identification of Bacillus acidi lactici
By the lactic acid bacillus mycopremna that preliminary screening goes out carry out respectively growth temperature experiment (10 DEG C, 15 DEG C, 45 DEG C, 60 DEG C,
30min), pH value gradient test, mobility test, catalase test, oxidase test, hydrogen sulfide production test, gelatin liquefaction are real
Test, nitrate reduction test, indole test, the experiment of glucose aerogenesis, benzidine test, litmus milk tests, arginine production ammonia
Experiment, casein decomposition run and various sugar alcohol fermentation tests.
As a result isolated from 10 parts of yak yogurt samples and be purified into 20 plants of Bacillus acidi lacticis, be Gram-positive,
Catalase test is negative.By morphological observation and physiological and biochemical test, this 20 plants of bacterial strains are initially identified as 7 kinds of lactic acid
Bacillus, number consecutively is Lb1~Lb7, wherein, numbering is Lb6 Bacillus acidi lactici is initially identified as Lactobacillus plantarum
(Lactobacillus plantarum) (table 1).
The numbering of table 1 is LbThe morphological feature and physiological and biochemical test result of 6 Bacillus acidi lactici
Note:+, it is positive;-, it is negative;+ w, weakly positive.
3rd, the identification of Lactobacillus plantarum
It is L to take numberingbSupernatant is abandoned in the bacteria suspension of 6 Bacillus acidi lactici Liquid Culture, centrifugation, thalline is collected, according to bacterium base
Because a group DNA extraction kit (Beijing Suo Laibao scientific & technical corporation) methods described extracts genomic DNA, Ago-Gel electricity is carried out
Swim (gel mass concentration ratio 0.8%, voltage 80V, electrophoresis 80min), after GelRed nucleic acid staining dyes, in gel imaging
(Fig. 1) is observed in system.The genomic DNA of extraction is diluted 200 times, concentration purity testing is carried out with ultraviolet specrophotometer.
Using primer 2 7F:5 '-AGAGTTTGATCCTGGCTCAG-3 ' (SEQ ID No.1) and 1492R: 5’-
GGCTACCTTGTTACGACTT-3 ' (SEQ ID No.2) enters performing PCR amplification (Fig. 2) to 16S rDNA genetic fragments.Amplification production
Thing is sequenced by Sangon Biotech (Shanghai) Co., Ltd. and Beijing Liuhe Huada Genomics Technology Co., Ltd, and will
Sequencing result (SEQ ID No.3) is compared with correlated series in Genbank.As a result show, numbering is Lb6 lactic acid bar
The similitude of the 16S rDNA gene fragment orders of bacterium and Lactobacillus plantarum strain CIP 103151 reaches
To 99%.
Therefore, numbering is Lb6 Bacillus acidi lactici is accredited as Lactobacillus plantarum (Lactobacillus plantarum), will
It is named as YS3, and is preserved in China typical culture collection center (abbreviation CCTCC, address on December 14th, 2016:Lake
No. 299 Wuhan Universitys of Bayi Road of Bei Sheng wuchang, wuhan area), deposit number is CCTCC NO:M2016749.
The prevention effect of embodiment 2, Lactobacillus plantarum YS3 Dui azolactone inducing mouse colitis
Main material, instrument and the experimental animal that the present embodiment is used are as follows:Lactobacillus plantarum YS3 (preserving numbers:CCTCC
NO:M2016749), lactobacillus bulgaricus (Chinese industrial Microbiological Culture Collection administrative center);Azolactone (U.S. Sigma
Company);MPO, NO, GSH, MDA, SOD determine kit (Bioengineering Research Institute is built up in Nanjing);IL-2, IL-10 serum are thin
Intracellular cytokine determines kit (BioLegend companies of the U.S.);Trizol reagents, OligodT18, RNase, dNTP, MLV are reversed
Record enzyme (Invitrogen companies of the U.S.);ROX reference Dye and SYBR Premix Ex Taq II (Japanese TAKARA
Company);RT-PCR primer (Beijing Tiangeng biochemical technology Co., Ltd).Biomate3S ultraviolet-visibles spectrophotometer,
The multi-functional ELIASA (Thermo Fisher Scientific companies of the U.S.) of A200PCR instrument, LUX;Tancon2500PCR
Gel imager (Shanghai Tian Neng Science and Technology Ltd.s);(the limited public affairs of instrument are contained to ICEN-24R high speed freezing centrifuges by Hangzhou Austria
Department).SPF grades of BALB/c (body weight 25-30g) mouse of male 7 week old purchased from Medical University Of Chongqing's animal experimental center, (permit by animal
Card number:SCXK (Chongqing) 2012-0001).
50 BALB/c mouses are randomly divided into 5 groups, are normal group, model control group respectively, at lactobacillus bulgaricus
Reason group, Lactobacillus plantarum YS3 low concentrations treatment group and high concentration treatment group, every group 10.Normal group and model control group are normal
Freely take in diet and drink water 2 weeks;During this is 2 weeks except it is normal freely take in diet and drinks water in addition to, at lactobacillus bulgaricus
The 1.0 × 10 of the daily every gavage 2mL of reason group mouse9At CFU/mL lactobacillus bulgaricus, Lactobacillus plantarum YS3 low concentrations
The 1 × 10 of reason group and the daily every difference gavage 2mL of high concentration treatment group mouse8CFU/mL Lactobacillus plantarum YS3 and 1 ×
109CFU/mL Lactobacillus plantarum YS3.The belly of the 1st day all mouse carries out shaving, normal group with 2 × 2cm areas after 2 weeks
Mouse web portion smears 0.2mL ethanol;It is 3% azolactone solution (ethanol that other each group mouse web portions, which smear 0.2mL mass ratioes,
For solvent).Implement fasting to mouse, but allow mouse freely to take in drinking-water within 5th day.Mouse is implemented after fasting 24h to anaesthetize
(0.1mL/10g chloraldurate), then carries out bowel lavage:With silicone tube it is blunt nosed from mouse anus insert enteron aisle depth 3.5cm, normally
The ethanol solution that group mouse injection 0.15mL volume ratios are 50%, other each group mouse injection 0.15mL mass ratioes are 1% Evil
Conduit is extracted after oxazolone solution (ethanol solution that volume ratio is 50% is solvent), 20s, while mouse is inverted into 30s.Bowel lavage 7
The neck that breaks after it puts to death whole mouse, collects mice plasma and colon is standby.
1st, influences of the Lactobacillus plantarum YS3 to colitis symptoms
(the 20th day), 3 days (the 22nd day), 6 days (the 25th day) the DAI standards of grading calculating as described in table 2 respectively 1 day after bowel lavage
DAI values, DAI=(Body weight loss fraction+stool fraction+fraction of having blood in stool)/3.Colitis can cause body weight loss simultaneously
There is diarrhoea and the symptom such as bleeding, can be as measurement colitis using body weight, stool and the DAI having blood in stool as score standard
The standard of degree.
The DAI standards of grading of table 2
The DAI value result of calculations of each group mouse are shown in Table 3, and DAI value of the normal group mouse in whole experimental period is remained
0.00 ± 0.00, DAI values increase and keep highest in each group always always after model control group mouse azolactone bowel lavage, protect
Plus the DAI values of Leah lactobacillus and Lactobacillus plantarum YS3 gavage Hou azolactone inducing colitis mouse are aobvious compared with model control group
Write and decline (p<0.05), wherein Lactobacillus plantarum YS3 high concentrations treatment group is reduced by up to.
The DAI values of each group mouse of table 3
Note:Alphabetical different expression group differences are significantly (P < 0.05).
Mouse Colon tissue is taken, the weight and length of colon is determined, the ratio of colon weight/colon lengths is calculated.Colon
The ratio of length and colon weight/colon lengths also weighs the standard of colitis degree.Compared with normal mouse, colon
The colon lengths of scorching mouse are shorter, colon weight/colon lengths ratios are lower.
The colon lengths of each group mouse and the ratio of colon weight/colon lengths are shown in Table 4, the colon of model control group mouse
The ratio of length and colon weight/colon lengths is minimum, normal group mouse highest, and Lactobacillus plantarum YS3 high concentrations are handled
The colon lengths of mouse and the ratio of colon weight/colon lengths are organized closest to normal group.
The colon lengths of each group mouse of table 4 and the ratio of colon weight/colon lengths
Note:Alphabetical different expression group differences are significantly (P < 0.05).
The mouse colitis symptom of low azolactone induction can drop in above-mentioned experimental result explanation, Lactobacillus plantarum YS3, and with
The increase effect for concentration becomes apparent, and its effect is better than the lactobacillus bulgaricus with concentration.
2nd, influences of the Lactobacillus plantarum YS3 to mouse Colon tissue MPO, NO, GSH and MDA content
The physiological saline of 9 times of quality is added in clean mouse Colon tissue, it is using Ultrasonic Pulverization that colon is even
Slurry, is then measured by kit specification to MPO, NO, GSH and MDA content in mouse Colon tissue.MPO is in colon
Content improve and mean that stream enters colon in neutrophil leucocyte, be the table that neutrophil accumulation is reduced in Inflamed tissue
It is existing.INOS generates the damage that NO aggravates colon during colitis, while increasing in colon with NO content
Plus MPO activity also strengthens therewith, inflammation aggravation.Colitis may further result in the big volume productions of ROS (active oxygen) and RNS (active nitrogen)
It is raw, histocyte is occurred toxic reaction, colon is inflamed damage.Be inflamed reaction after ROS a large amount of generations
The content of GSH in body oxidation/anti-oxidant balance, reduction colon will be destroyed, substantial amounts of peroxidatic reaction of lipid aggravates fat
Matter peroxidating end-product MDA generation.
As shown in Table 5, MPO, NO, MDA content highest in model control group mouse Colon tissue, GSH contents are minimum;Just
Often the colon of group mouse shows opposite trend, and MPO, NO, MDA content are minimum, GSH content highests;Relative to model
Control group, lactobacillus bulgaricus and Lactobacillus plantarum YS3 can reduce MPO in colitis mice colon, NO,
MDA contents and raising GSH contents, but Lactobacillus plantarum YS3 effect is stronger.
MPO, NO, GSH and MDA content of each group mouse Colon tissue of table 5
Note:Alphabetical different expression group differences are significantly (P < 0.05).
3rd, influences of the Lactobacillus plantarum YS3 to mice serum cell factor IL-2 and IL-10 level
Mice serum is taken, IL-2 and IL-10 cell factors in mice serum are determined by kit specification using ELISA method
Content.The colitis of azolactone induction is a kind of cell-mediated inflammation of Th2.The mechanism that inflammation is produced is between Th2/Th1
Immunological network unbalance cause colitis.IL-10 is the cell factor that a kind of Th2 cells are produced, and IL-2 is that Th1 cells are produced
Cell factor, all directly related with colitis, too low IL-2 levels and too high IL-10 levels can mean that colitis degree
Deepen.
As shown in Table 6, normal group mice serum cell factor IL-2 levels are significantly higher than other group of mouse (p<0.05), and
IL-10 levels are substantially less than other group of mouse (p<0.05);Compared with other each groups, Lactobacillus plantarum YS3 high concentration treatment groups
The closest normal group of mice serum cell factor IL-2 and IL-10 level.
Each group mice serum cell factor IL-2 and the IL-10 level of table 6
Note:Alphabetical different expression group differences are significantly (P < 0.05).
4th, the influence that Lactobacillus plantarum YS3 is expressed mouse Colon tissue correlation mRNA
The colon of mouse is taken, colon's total serum IgE is extracted with RNAzol after crushing, is diluted to 1 μ g/ μ L;Take 2 μ L dilute
Total RNAs extraction liquid after releasing, sequentially adds 1 μ L oligodT18, RNase, dNTP, MLV enzyme and 10 μ L 5 × buffer,
37 DEG C of 120min, 99 DEG C of 4min, 4 DEG C of 3min synthesis cDNA;Then use primer described in table 7, RT-PCR amplification nNOS, eNOS,
INOS, c-Kit, SCF, IL-8 and CXCR2 mRNA expression, internal reference is used as using housekeeping gene GAPDH;Finally with containing mass ratio
Agar electrophoresis for 1% ethidium bromide checks pcr amplification product, and carries out semi-quantitative analysis with Image1.44 softwares.
The RT-PCR primer sequence of table 7
(1) influence that Lactobacillus plantarum YS3 is expressed mouse Colon tissue nNOS, eNOS and iNOS mRNA
NOS points are nNOS, eNOS and iNOS, and research confirms that the NO that eNOS is produced has crucial work to the damage of control colon
With the excessive NO that iNOS is produced then accelerates colitis to damage, and nNOS, which is lowered, also makes iNOS expression strengthen a large amount of release NO simultaneously.
From Fig. 3 and table 8, nNOS, eNOS mRNA expression highests of normal group mouse Colon tissue, and iNOS mRNA
Expression it is minimum;NNOS, eNOS mRNA of model control group mouse express minimum, iNOS mRNA expression highest;Relative to
Model control group, lactobacillus bulgaricus and Lactobacillus plantarum YS3 can be raised significantly in colitis mice colon
NNOS, eNOS mRNA expression and the expression (p for lowering iNOS mRNA<0.05), but Lactobacillus plantarum YS3 effect it is stronger.
Semi-quantitative analysis (the relative model control group table of table 8 mouse Colon tissue nNOS, eNOS and iNOS mRNA expression
Up to multiple)
Note:Alphabetical different expression group differences are significantly (P < 0.05).
(2) influence that Lactobacillus plantarum YS3 is expressed mouse Colon tissue c-Kit and SCF mRNA
Ulcerative colitis not only shows to suffer from diarrhoea and have blood in stool, while also there is colonic motor disorder.Research has shown that ICC
(Cajal interstitial cells) is relevant with colonic activity, directly participates in colitis process.When there is IBD, SCF is to maintaining
ICC quantity and function have direct effect, and SCF is c-Kit natural ligand, ICC increasing after SCF/Kit signal pathways sustain damage
Growing and breaking up can decline, so as to aggravate colitis.
From Fig. 4 and table 9, relative to model control group, lactobacillus bulgaricus and Lactobacillus plantarum YS3 can show
Write the expression (p of c-Kit and SCF mRNA in up-regulation colitis mice colon<0.05), but Lactobacillus plantarum YS3 effect
It is stronger.
Semi-quantitative analysis (the relative model control group expression times of table 9 mouse Colon tissue c-Kit and SCF mRNA expression
Number)
Note:Alphabetical different expression group differences are significantly (P < 0.05).
(3) influence that Lactobacillus plantarum YS3 is expressed mouse Colon tissue IL-8 and CXCR2mRNA
IL-8 has inflammatory activity and chemotaxis, and CXCR is IL-8 acceptors, and the morbidity of IL-8 and CXCR2 with colon cancer has
Close, document also reports the high expression that IL-8 and CXCR2 occurs under colon cancer state.
From Fig. 5 and table 10, relative to model control group, lactobacillus bulgaricus and Lactobacillus plantarum YS3 can
Significantly lower the expression (p of IL-8 and CXCR2mRNA in colitis mice colon<, but Lactobacillus plantarum YS3 0.05)
Effect is stronger.
Semi-quantitative analysis (the relative model control group expression times of table 10 mouse Colon tissue IL-8 and CXCR2mRNA expression
Number)
Note:Alphabetical different expression group differences are significantly (P < 0.05).
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although pass through ginseng
According to the preferred embodiments of the present invention, invention has been described, it should be appreciated by those of ordinary skill in the art that can
So that various changes are made to it in the form and details, the present invention limited without departing from appended claims
Spirit and scope.
<110>The college of education of Chongqing second
<120>Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared
<160>19
<210>1
<211>20
<212>DNA
<213>Artificial sequence
<220>
<223>Primer 2 7F
<400>1
agagtttgat cctggctcag 20
<210>2
<211>19
<212>DNA
<213>Artificial sequence
<220>
<223>Primer 1492R
<400>2
ggctaccttg ttacgactt 19
<210>3
<211>1466
<212>DNA
<213>Lactobacillus plantarum YS3(Lactobacillus plantarum YS3)
<220>
<223>16S rDNA genetic fragments
<400>3
taatacatgc aagtcgaacg aactctggat tgattggtgc ttgcatcatg atttacattt 60
gagggagtgg cgaacgtgca atggcggctt tggtgagtaa cacgtgggaa acctgcccag 120
aagcggggaa taacacctgg aaacagatgc tataccgcat aacaacttgg accgcatggt 180
ccgagnttga aagatggctt cggctatcac ttttggatgg tcccgcggcg tattagctag 240
atggtggggt aacggctcac catggcaatg atacgtagcc gacctgagag ggtaatcggc 300
cacattggga ctgactcacg gccaaactcc tacgggaggc agcagtaggg aatcttccac 360
aatggacgaa gtctgatgga gcaacgccgc gtgagtgaaa agggtttcgg ctcgtaaact 420
ctgttgttaa agaagaacat atctgagagt aactgttcag gtattgacgg tatttaacag 480
aaagccacgg ctaactacgt gccagcagcc gcggtaatac gtaggtggca agcgttgtcc 540
ggatttattg ggcgtaaagc gagcgcaggc ggttttttaa gtctgatgtg aaagccttcg 600
gctcaaccga agaagtgcat cggaaatggg aaacttgagt gcagaagagg acagtggaac 660
tccattgtag cggtgaaatg cgtagatata tggaagaaca ccagtggcga aggcgctgtc 720
tggtctgtaa ctgacgctga ggctcgaaag tatggtagca aacaggatta gataccctgg 780
tatccatacc gtaaacgatg aatgctaagt gttggagggt ttccgccctt cagtgctgca 840
gctaacgcat taagcattcc gcctggggag tacggccgca aggctgaaac tcaaaggaat 900
tacgggggcc cgcacaagcg gtggagcatg tggttaaatt cgaagctacg cgaagaacct 960
taccaggtct tgacatacta tgcaaatcta agagattaga cgttcccttc ggggacatgg 1020
atacaggtgg tgcatggttg tcgtcagctc atgtcgtgag atgttgggtt agtcccgcac 1080
gagcgcaacc cttattatca gttgccagcg ttaagttggg cactctggtg agactgccgg 1140
tgacaaacgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta 1200
cacacgtgct acaatggatg gtacaacagt tgcgaactcg cgagagtaag ctaatctctt 1260
aaagccattc tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta 1320
gtaatccgga tcagcatgcc gcggtgaata cgttcccggg ccttgacaca ccgcccgtca 1380
caccatgaga gtttgtaaca cccaaagtcg gtggggtaac cttttaggaa ccagccgcct 1440
aagggggaca gtagagtaag gtggat 1466
<210>4
<211>23
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification nNOS mRNA expression
<400>4
gaataccagc ctgatccatg gaa 23
<210>5
<211>26
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification nNOS mRNA expression
<400>5
tcctccagga gggtgtccac cgcatg 26
<210>6
<211>20
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification eNOS mRNA expression
<400>6
ggagaggctg catgacattg 20
<210>7
<211>22
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification eNOS mRNA expression
<400>7
ggtagagcca tagtggaatg ac 22
<210>8
<211>18
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification iNOS mRNA expression
<400>8
agagagatcg ggttcaca 18
<210>9
<211>18
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification iNOS mRNA expression
<400>9
cacagaactg agggtaca 18
<210>10
<211>16
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification c-Kit mRNA expression
<400>10
agaccgaacg caactt 16
<210>11
<211>16
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification c-Kit mRNA expression
<400>11
ggtgccatcc acttca 16
<210>12
<211>16
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification SCF mRNA expression
<400>12
aaactggtgg cgaatc 16
<210>13
<211>16
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification SCF mRNA expression
<400>13
cacgggtagc aagaac 16
<210>14
<211>21
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification IL-8 mRNA expression
<400>14
agaagcatgg cccagaaatc a 21
<210>15
<211>20
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification IL-8 mRNA expression
<400>15
ggccttgtag acaccttggt 20
<210>16
<211>18
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification CXCR2 mRNA expression
<400>16
gaacaaaggc aaggctaa 18
<210>17
<211>20
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification CXCR2 mRNA expression
<400>17
aacataacaa catctgggca 20
<210>18
<211>20
<212>DNA
<213>Artificial sequence
<220>
<223>The sense primer of RT-PCR amplification GAPDH mRNA expression
<400>18
cggagtcaac ggatttggtc 20
<210>19
<211>20
<212>DNA
<213>Artificial sequence
<220>
<223>The anti-sense primer of RT-PCR amplification GAPDH mRNA expression
<400>19
agccttctcc atggtcgtga 20
Claims (1)
1. deposit number is CCTCC NO:M2016749 Lactobacillus plantarum YS3 (Lactobacillus plantarum YS3)
Application in the food or medicine of prevention ulcerative colitis is prepared.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710542494.XA CN107319564A (en) | 2017-07-05 | 2017-07-05 | Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710542494.XA CN107319564A (en) | 2017-07-05 | 2017-07-05 | Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107319564A true CN107319564A (en) | 2017-11-07 |
Family
ID=60195707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710542494.XA Pending CN107319564A (en) | 2017-07-05 | 2017-07-05 | Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107319564A (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014193014A1 (en) * | 2013-05-29 | 2014-12-04 | 바이오제닉스코리아 주식회사 | Nano-sized kimchi lactic acid bacteria |
CN105106246A (en) * | 2015-08-20 | 2015-12-02 | 江南大学 | Lactobacillus plantarum ZS2058 and application thereof |
CN105343133A (en) * | 2015-12-08 | 2016-02-24 | 东北农业大学 | Compound probiotics and drug for treating ulcerative colitis and preparation method thereof |
-
2017
- 2017-07-05 CN CN201710542494.XA patent/CN107319564A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014193014A1 (en) * | 2013-05-29 | 2014-12-04 | 바이오제닉스코리아 주식회사 | Nano-sized kimchi lactic acid bacteria |
CN105106246A (en) * | 2015-08-20 | 2015-12-02 | 江南大学 | Lactobacillus plantarum ZS2058 and application thereof |
CN105343133A (en) * | 2015-12-08 | 2016-02-24 | 东北农业大学 | Compound probiotics and drug for treating ulcerative colitis and preparation method thereof |
Non-Patent Citations (2)
Title |
---|
叶胜青等: "植物乳杆菌对2,4,6-三硝基苯磺酸诱导的小鼠结肠炎的治疗作用", 《世界华人消化杂志》 * |
孙鹏等: "植物乳杆菌YS1对恶唑酮诱导BALB/c小鼠结肠炎的预防作用", 《食品工业科技》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111235070B (en) | Breast milk infant source lactobacillus plantarum BF _15 and application thereof | |
CN107028988A (en) | Applications of the Lactobacillus plantarum YS1 in the food or medicine of prevention ulcerative colitis is prepared | |
CN109234189A (en) | One plant of lactobacillus plantarum strain BX62 and its application with oxidation resistance | |
CN109182162B (en) | Lactobacillus plantarum with antioxidant capacity and application thereof | |
CN107099492B (en) | Lactobacillus plantarum YS4 and application thereof in preparation of constipation preventing food | |
CN102031229A (en) | Lactobacillus plantarum with gastrointestinal adsorption capacity and cholesterol removing capacity | |
CN107227278A (en) | A kind of Lactobacillus plantarum A11 and its application | |
CN107338198A (en) | A kind of Lactobacillus plantarum and its application | |
Belhadj et al. | Phenotypic and genotypic characterization of some lactic acid bacteria isolated from bee pollen: a preliminary study | |
CN109662976A (en) | A kind of application of Lactobacillus rhamnosus in the health food and drug of preparation prevention ulcerative colitis | |
CN109481476A (en) | Application of the lactobacillus fermenti CQPC04 in the food or drug that preparation improves ulcerative colitis | |
CN106906165B (en) | Lactobacillus plantarum and application thereof in preparation of food for preventing constipation | |
CN109666614A (en) | A kind of Lactobacillus rhamnosus and its application in the health food and drug of preparation prevention allergic asthma | |
CN113265351A (en) | Lactobacillus W8172 and application thereof | |
CN107223984A (en) | Applications of the Lactobacillus plantarum YS4 in the food or medicine of prevention ulcerative colitis is prepared | |
CN106811436A (en) | One lactobacillus plantarum and its application in correction Social behaviors preparation is prepared | |
Zamanpour et al. | Isolation and some basic characteristics of lactic acid bacteria from beetroot (Beta vulgaris L.)—A preliminary study | |
CN104877940B (en) | One plant of streptococcus thermophilus | |
CN107099491A (en) | Lactobacillus plantarum YS3 and its application in the food of Constipation is prepared | |
CN113897300A (en) | Bifidobacterium animalis capable of improving skin barrier function damage and skin sensitivity | |
CN107177535A (en) | Applications of the Lactobacillus plantarum YS2 in the food or medicine of prevention ulcerative colitis is prepared | |
Delgado et al. | Bifidobacterial diversity determined by culturing and by 16S rDNA sequence analysis in feces and mucosa from ten healthy Spanish adults | |
CN107319564A (en) | Applications of the Lactobacillus plantarum YS3 in the food or medicine of prevention ulcerative colitis is prepared | |
CN112694985B (en) | Lactobacillus fermentum TCI275, and composition and use thereof | |
CN112300962B (en) | Lactobacillus plantarum with specific molecular target and antioxidation function and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171107 |