CN107312515B - A kind of multi-element biologic compound oil displacement agent system and its injection technology - Google Patents
A kind of multi-element biologic compound oil displacement agent system and its injection technology Download PDFInfo
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- CN107312515B CN107312515B CN201710405104.4A CN201710405104A CN107312515B CN 107312515 B CN107312515 B CN 107312515B CN 201710405104 A CN201710405104 A CN 201710405104A CN 107312515 B CN107312515 B CN 107312515B
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- 238000006073 displacement reaction Methods 0.000 title claims abstract description 48
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 41
- 150000001875 compounds Chemical class 0.000 title claims abstract description 37
- 238000002347 injection Methods 0.000 title abstract description 23
- 239000007924 injection Substances 0.000 title abstract description 23
- 238000005516 engineering process Methods 0.000 title abstract description 16
- 230000015556 catabolic process Effects 0.000 claims abstract description 43
- 238000006731 degradation reaction Methods 0.000 claims abstract description 43
- 239000003209 petroleum derivative Substances 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 18
- 230000008569 process Effects 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims description 125
- 241000894006 Bacteria Species 0.000 claims description 106
- 238000000855 fermentation Methods 0.000 claims description 89
- 230000004151 fermentation Effects 0.000 claims description 89
- 239000002054 inoculum Substances 0.000 claims description 31
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 28
- 238000002156 mixing Methods 0.000 claims description 21
- 231100000219 mutagenic Toxicity 0.000 claims description 20
- 230000003505 mutagenic effect Effects 0.000 claims description 20
- 235000015097 nutrients Nutrition 0.000 claims description 20
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 18
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 15
- 230000004913 activation Effects 0.000 claims description 15
- 229920001222 biopolymer Polymers 0.000 claims description 15
- 238000009423 ventilation Methods 0.000 claims description 15
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 14
- 238000010899 nucleation Methods 0.000 claims description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 13
- 239000001110 calcium chloride Substances 0.000 claims description 13
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 13
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 13
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 13
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 13
- 238000011081 inoculation Methods 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 10
- DCAYPVUWAIABOU-UHFFFAOYSA-N hexadecane Chemical compound CCCCCCCCCCCCCCCC DCAYPVUWAIABOU-UHFFFAOYSA-N 0.000 claims description 10
- 230000006698 induction Effects 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 10
- 230000035772 mutation Effects 0.000 claims description 10
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 10
- 239000002356 single layer Substances 0.000 claims description 10
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 239000004094 surface-active agent Substances 0.000 claims description 10
- 239000006188 syrup Substances 0.000 claims description 10
- 235000020357 syrup Nutrition 0.000 claims description 10
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 9
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 9
- 235000019270 ammonium chloride Nutrition 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 9
- 229940057995 liquid paraffin Drugs 0.000 claims description 9
- 239000011565 manganese chloride Substances 0.000 claims description 9
- 235000002867 manganese chloride Nutrition 0.000 claims description 9
- 229940099607 manganese chloride Drugs 0.000 claims description 9
- 238000003359 percent control normalization Methods 0.000 claims description 9
- 238000011534 incubation Methods 0.000 claims description 8
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 239000004021 humic acid Substances 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 6
- 235000011164 potassium chloride Nutrition 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 241000589636 Xanthomonas campestris Species 0.000 claims description 5
- 150000004945 aromatic hydrocarbons Chemical class 0.000 claims description 5
- 229960003237 betaine Drugs 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 150000001720 carbohydrates Chemical class 0.000 claims description 5
- 150000001924 cycloalkanes Chemical class 0.000 claims description 5
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 5
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 235000010344 sodium nitrate Nutrition 0.000 claims description 5
- 239000004317 sodium nitrate Substances 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 4
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 4
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 4
- 229920001285 xanthan gum Polymers 0.000 claims description 4
- 239000000230 xanthan gum Substances 0.000 claims description 4
- 229940082509 xanthan gum Drugs 0.000 claims description 4
- 235000010493 xanthan gum Nutrition 0.000 claims description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 238000011084 recovery Methods 0.000 abstract description 19
- 238000000605 extraction Methods 0.000 abstract description 5
- 238000011017 operating method Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 56
- 239000003921 oil Substances 0.000 description 47
- 238000002474 experimental method Methods 0.000 description 7
- 239000003814 drug Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 4
- 239000011206 ternary composite Substances 0.000 description 4
- 239000004215 Carbon black (E152) Substances 0.000 description 3
- 238000007664 blowing Methods 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- 239000012263 liquid product Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 239000010779 crude oil Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2208/00—Aspects relating to compositions of drilling or well treatment fluids
- C09K2208/12—Swell inhibition, i.e. using additives to drilling or well treatment fluids for inhibiting clay or shale swelling or disintegrating
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Mining & Mineral Resources (AREA)
- Geology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- Geochemistry & Mineralogy (AREA)
- Environmental & Geological Engineering (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Of the invention a kind of multi-element biologic compound oil displacement agent system and its injection technology, it is related to oil reservoir oil displacement agent and process for producing same and application method, its oil displacement system is made of petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent, its injection technology operating procedure are as follows: by block well injection station, using injection well and extraction well as defined area, biological compound oil displacement agent 0.2pv is injected by with injection allocation flow velocity and with fluence requirement, then note petroleum hydrocarbon degradation microbial inoculum 0.1pv is taken over, take over the biological compound oil displacement agent 0.2pv of injection, continue to inject petroleum hydrocarbon degradation microbial inoculum 0.1pv, finally inject biological compound oil displacement agent 0.2pv, finally use water drive instead i.e..The present invention after water drive hypotonic heavy crude reservoir, it is at low cost, pollution-free, do not injure oil reservoir;Atmospheric pressure is produced using this system and injection technology, after 76 hours up to 1.0MP or more, system is diluted to concentration 5~10%, and interfacial tension reaches 5.2 × 10‑3~8.2 × 10‑3MN/m, to viscosity in the viscous crude of 10000 mpas, viscosity break ratio reaches 85.7%, and physical analogy improves recovery ratio and averagely reaches 18.0% or more.
Description
Technical field
The present invention relates to a kind of production method of oil reservoir oil displacement agent and application method more particularly to a kind of multi-element biologic are compound
Oil displacement agent system and its injection technology.
Background technique
Petroleum Industry is one of pillar industry of Chinese national economy, has great shadow to the development of Chinese national economy
It rings.Oilfield exploitation experienced primary oil recovery, secondary oil recovery and tertiary oil recovery, tertiary oil recovery include heating power, chemistry, the miscible displacement of reservoir,
Four class technology of microbe oil production, wherein Microbial Enhanced Oil Recovery is at low cost, does not pollute stratum and environment, is one very potential
Raising harvest efficiency technology.Just currently, chemical ternary composite driving technology is both at home and abroad at one of tertiary phase exploitation
Recovery efficiency technique is efficiently improved, but there are high concentration alkali corrosion and scaling, demulsification difficulty, dosage of surfactant are big, at high cost, poly-
The problems such as closing object difficult to degrade and its waste water treatmentntrol difficult.Life is carried out in irrational extraction of resources for chemical ternary composite driving to oil reservoir
Polymer, alkali and chemical table in chemical ternary composite displacement system are replaced in the research of object multiple elements design oil displacement system with biological product
Face activating agent forms degradable, pollution-free and efficient biological oil displacement system, forms efficient green in the following oil field development and adopt
Oil tech is of great significance.
Summary of the invention
The present invention provides a kind of multi-element biologic compound oil displacement agent system and its injection for above-mentioned the deficiencies in the prior art
Technique.
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes, the petroleum hydrocarbon degradation microbial inoculum is mixed by 1~5 part of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 15~20 portions of nutrient solutions
It closes and is uniformly made;
The biological compound oil displacement agent is made of by weight following ingredients: biopolymer bacterium fermenation raw liquid 5~
10 parts, biological 10~20 parts, 1~5 part of glycine betaine of surfactant of lipopeptid, control pH8~9 by 60~80 parts of water;
The nutrient solution is 0.5~5 part of mixed liquor, the phosphoric acid hydrogen two by corn syrup and sucrose by 3:1 quality proportioning
0.1~0.5 part of ammonium, 0.1~0.5 part of sodium nitrate, 0.02~0.05 part of humic acid sylvite, 60~80 parts of water;
It is above mass fraction.
As a further improvement of the present invention, the petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, from
The heart removes confluent monolayer cells, washs cell, is resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25
DEG C overnight incubation, dilutes coated plate, and selection can utilize hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
For single carbon source for growth, be target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 18~24 hours, activation bacterium solution is made;
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2~3 parts of ammonium chloride, potassium dihydrogen phosphate
1.5~2 parts, 0.5~1 part of epsom salt, 0.1~0.5 part of manganese chloride, 0.2~0.5 part of calcium chloride, liquid amount control 60
~80%, it is above mass fraction;
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24~30 hours, obtains seed bacterium solution;
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control cultivates 24~36 7.0~8.0,25 ± 2 DEG C of temperature
After hour, primary fermentation liquid is made;
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control cultivate 30 7.0~8.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
After~48 hours, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2~3 parts of ammonium chloride, di(2-ethylhexyl)phosphate
1.5~2 parts of hydrogen potassium, 0.5~1 part of epsom salt, 0.1~0.5 part of manganese chloride, 0.2~0.5 part of calcium chloride, liquid amount control
60~80%;
It is above mass fraction, mass percent and mass ratio.
As a further improvement of the present invention, the biopolymer bacterium fermenation raw liquid is obtained by the following method
:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min,
Confluent monolayer cells under centrifuging and taking wash cell, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml,
25 DEG C of overnight incubations dilute coated plate, and selection can be that carbon source generates Huang using carbohydrate at a temperature of 20~50 DEG C after screening domestication
The strain of virgin rubber is target strain;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, activation bacterium solution is made;
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, obtains seed bacterium solution;
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 7.0~8.0, temperature
After 25 ± 2 DEG C, culture 8~13 hours, one grade fermemtation bacterium solution is made;
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control 0.5~0.6vvm of ventilation quantity, 100 revs/min of speed of agitator, pH value control 7.0~8.0,
After 25 ± 2 DEG C of temperature, culture 8~13 hours, second order fermentation bacterium solution is obtained;
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, 0.7~0.8vvm of ventilation quantity, process pH value control are stirred 7.0~8.5,25 ± 2 DEG C of temperature
80 revs/min of speed, culture 7~10 hours after, will fermentation liquid import same volume fermentor in press same process parameter, respectively after
Biopolymer bacterium fermenation raw liquid is made after fermentation 15~20 hours in continuous fermenting and producing;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
10~20 parts, 5~7 parts of yeast extract, 0.2~0.3 part of peptone, 1.5~2 parts of potassium dihydrogen phosphate, epsom salt 0.3~0.5
At 25 ± 2 DEG C of 20~50%, cultivation temperature, pH value 7~8, mixing speed is controlled for part, the control of 0.1~0.2 part of calcium chloride, liquid amount
It is above mass fraction in 80~150rpm;
It is above mass fraction, mass percent and mass ratio.
A kind of injection technology of multi-element biologic compound oil displacement agent system, specific steps are as follows: being injected by block well
It stands, using injection well and extraction well as defined area, injects biological compound oil displacement agent 0.2pv by injection allocation flow velocity and with fluence requirement,
Then note petroleum hydrocarbon degradation microbial inoculum 0.1pv is taken over, the biological compound oil displacement agent 0.2pv of injection is taken over, continues to inject petroleum hydrocarbon degradation
Microbial inoculum 0.1pv finally injects biological compound oil displacement agent 0.2pv, and finally using water drive instead is to complete a full set of injection technology.
Wherein, the preparation method of the biological lipopeptid surfactant in biological compound oil displacement agent be public technology, by
A kind of patent of invention " industrialized process for preparing of the lipopeptide biosurfactant " institute of Patent No. 201110343413.6 is public
It opens.
A kind of multi-element biologic compound oil displacement agent system of the invention, has the advantage that
1) its pH is neutral or alkalescent, after water drive hypotonic heavy crude reservoir, have it is at low cost, pollution-free,
Not the characteristics of not injuring oil reservoir, sustainable oil recovery;
2) it is with endogenous and inoculating microbe oil recovery technique performance, while having both chemical ternary composite driving mechanism,
While driving crude oil, using gas effect, washing oil effect and swept volume effect is produced, promote the extraction of crude oil;
3) petroleum hydrocarbon degradation bacterium is tamed by screening, generates biological polyoses polymer, yield is up to 2.5~3.5%;
4) biological compound oil displacement agent not only can be used as the nutriment of petroleum hydrocarbon degradation bacterium, but also has and increase swept volume,
Liquid mobility is reduced to be compared to use;
5) humic acid sylvite had both been used as microbial nutrition agent, and the effect for promoting viscosity of crude to reduce, which again has system, to be prevented
Clay swell effect;
6) this system and injection technology are utilized, atmospheric pressure is produced after 76 hours up to 1.0MP or more, system is diluted to concentration 5
~10%, interfacial tension reaches 5.2 × 10-3~8.2 × 10-3MN/m, to viscosity in the viscous crude of 10000 mpas, viscosity break ratio reaches
To 85.7%, physical analogy improves recovery ratio and averagely reaches 18.0% or more.
Specific embodiment
Embodiment 1
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes,
The petroleum hydrocarbon degradation microbial inoculum is by 1 part of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 15 portions of nutrient solutions, and mixing is equal
It is even to be made;
The biological compound oil displacement agent is made of by weight following ingredients: 5 parts of biopolymer bacterium fermenation raw liquid,
10 parts of biological lipopeptid surfactant, 1 part of glycine betaine, control pH8, system viscosity 47mpas by 60~80 parts of water;
The nutrient solution is 0.5 part of mixed liquor, the diammonium hydrogen phosphate by corn syrup and sucrose by 3:1 quality proportioning
0.1 part, 0.1 part of sodium nitrate, 0.02 part of humic acid sylvite, 60 parts of water;
It is above mass fraction.
Wherein, petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 600 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, centrifuging and taking
Lower confluent monolayer cells wash cell, in LB(Luria-Bertani) be resuspended in culture medium, its cell concentration is 1.0 × 108~2.0 ×
108A/ml, 25 DEG C of overnight incubations, dilute coated plate, selection can under the conditions of 10 DEG C~25 DEG C of temperature, using hexadecane,
Benzene, phenanthrene and hexamethylene are single carbon source for growth, are target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 18 hours, activation bacterium solution is made;
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2 parts of ammonium chloride, potassium dihydrogen phosphate 1.5
Part, the control of 0.5 part of epsom salt, 0.1 part of manganese chloride, 0.2 part of calcium chloride, liquid amount are mass fraction 60% above;
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24 hours, obtains seed bacterium solution;
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is after 7.0,25 ± 2 DEG C of temperature, culture 24 hours, system
Obtain primary fermentation liquid;
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control are cultivated 30 hours 7.0,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
Afterwards, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made, wherein surfactant hydrocarbon degradation bacteria bacterium number is 2 × 108A/ml or more;
It is above mass fraction, mass percent and mass ratio;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.5 part of liquid paraffin, 2 parts of ammonium chloride, potassium dihydrogen phosphate
1.5 parts, 0.5 part of epsom salt, 0.1 part of manganese chloride, 0.2 part of calcium chloride, liquid amount control in 40 DEG C of 80%, cultivation temperature, no
Blowing air, mixing speed control are mass fraction in 50pm above.
The biopolymer bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 600 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, is centrifuged
Remove confluent monolayer cells, cell washed, in LB(Luria-Bertani) be resuspended in culture medium, its cell concentration is 1.0 × 108~2.0
×108A/ml, 25 DEG C of overnight incubations dilute coated plate, and selection can be carbon using carbohydrate at a temperature of 20 DEG C after screening domestication
The strain that source generates xanthan gum is target strain;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10 hours, activation bacterium solution is made;
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10 hours, obtains seed bacterium solution;
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 7.0, temperature 25 ± 2
DEG C, after culture 8 hours, one grade fermemtation bacterium solution is made;
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 100 revs/min of speed of agitator, pH value control is 7.0, temperature 25 ± 2
DEG C, after culture 8 hours, obtain second order fermentation bacterium solution;
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, ventilation quantity 0.7vvm, process pH value control 7.0,25 ± 2 DEG C of temperature, 80 turns of mixing speed/
Point, after culture 7 hours, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermenting and producing respectively, sent out
After ferment 15 hours, biopolymer bacterium fermenation raw liquid is made, wherein microbe composite polysaccharide accounts in microbial fermentation liquid product
0.8%~1.1%;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
10 parts, 5 parts of yeast extract, 0.2 part of peptone, 1.5 parts of potassium dihydrogen phosphate, 0.3 part of epsom salt, 0.1 part of calcium chloride, liquid amount
At 25 ± 2 DEG C of 20%, cultivation temperature, pH value 7, mixing speed is controlled in 80rpm for control;
It is above mass fraction, mass percent and mass ratio.
Embodiment 2
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes,
The petroleum hydrocarbon degradation microbial inoculum is by 5 parts of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 20 portions of nutrient solutions, and mixing is equal
It is even to be made;
The biological compound oil displacement agent is made of by weight following ingredients: biopolymer bacterium fermenation raw liquid 10
Part, biological 20 parts, 5 parts of glycine betaine of surfactant of lipopeptid, control pH9, system viscosity 78mpas by 60~80 parts of water;
The nutrient solution is 5 parts of mixed liquor, the diammonium hydrogen phosphate 0.5 by corn syrup and sucrose by 3:1 quality proportioning
Part, 0.5 part of sodium nitrate, 0.05 part of humic acid sylvite, 80 parts of water;
It is above mass fraction.
Wherein, petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, from
The heart removes confluent monolayer cells, washs cell, is resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25
DEG C overnight incubation, dilutes coated plate, and selection can utilize hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
For single carbon source for growth, be target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24 hours, activation bacterium solution is made;
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 3 parts of ammonium chloride, potassium dihydrogen phosphate 2
Part, the control of 1 part of epsom salt, 0.5 part of manganese chloride, 0.5 part of calcium chloride, liquid amount are mass fraction 80% above;
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 30 hours, obtains seed bacterium solution;
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is cultivated 24~36 hours 8.0,25 ± 2 DEG C of temperature
Afterwards, primary fermentation liquid is made;
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control are cultivated 48 hours 8.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
Afterwards, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made, wherein surfactant hydrocarbon degradation bacteria bacterium number is 2 × 108A/ml or more;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 1 part of liquid paraffin, 3 parts of ammonium chloride, 2 parts of potassium dihydrogen phosphate,
1 part of epsom salt, 0.5 part of manganese chloride, 0.5 part of calcium chloride, liquid amount control 45 DEG C of 80%, cultivation temperature, not blowing air,
Mixing speed is controlled in 80rpm;
It is above mass fraction, mass percent and mass ratio.
The biopolymer bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 750 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, is centrifuged
Confluent monolayer cells are removed, cell is washed, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C
Overnight incubation dilutes coated plate, and selection can be at 50 °C the bacterium that carbon source generates xanthan gum using carbohydrate after screening domestication
Kind is target strain;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 115 hours, activation bacterium solution is made;
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 15 hours, obtains seed bacterium solution;
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 8.0, temperature 25 ± 2
DEG C, after culture 13 hours, one grade fermemtation bacterium solution is made;
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control ventilation quantity 0.6vvm, and 100 revs/min of speed of agitator, pH value control is 8.0, temperature 25 ± 2
DEG C, after culture 13 hours, obtain second order fermentation bacterium solution;
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, ventilation quantity 0.8vvm, process pH value control 8.5,25 ± 2 DEG C of temperature, 80 turns of mixing speed/
Point, after culture 10 hour, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermenting and producing respectively,
After fermentation 20 hours, biopolymer bacterium fermenation raw liquid is made, wherein microbe composite polysaccharide is in microbial fermentation liquid product
Account for 0.8%~1.1%;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
20 parts, 7 parts of yeast extract, 0.3 part of peptone, 2 parts of potassium dihydrogen phosphate, 0.5 part of epsom salt, 0.2 part of calcium chloride, liquid amount control
At 25 ± 2 DEG C of 50%, cultivation temperature, pH value 8, mixing speed is controlled in 150rpm system;
It is above mass fraction, mass percent and mass ratio.
Embodiment 3
A kind of multi-element biologic compound oil displacement agent system of the invention is by petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent
It constitutes,
The petroleum hydrocarbon degradation microbial inoculum is by 3 parts of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 18 portions of nutrient solutions, and mixing is equal
It is even to be made;
The biological compound oil displacement agent is made of by weight following ingredients: 8 parts of biopolymer bacterium fermenation raw liquid,
15 parts of biological lipopeptid surfactant, 3 parts of glycine betaine, control pH8.5, system viscosity 65mpas by 60~80 parts of water;
The nutrient solution is 3 parts of mixed liquor, the diammonium hydrogen phosphate 0.3 by corn syrup and sucrose by 3:1 quality proportioning
Part, 0.3 part of sodium nitrate, 0.05 part of humic acid sylvite, 70 parts of water;
It is above mass fraction.
Wherein, petroleum hydrocarbon degradation bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, so
Fermentation liquid to be mutagenic is subjected to 700 mcg/ml NTG processing afterwards, is mixed according to 1:1,25 DEG C of heat preservation 30min, centrifuging and taking
Lower confluent monolayer cells wash cell, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C of trainings
It supports overnight, dilutes coated plate, selection can be single using hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
One carbon source for growth is target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 300ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 20 hours, activation bacterium solution is made;
Shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2.5 parts of ammonium chloride, potassium dihydrogen phosphate
1.8 parts, 0.8 part of epsom salt, 0.3 part of manganese chloride, 0.3 part of calcium chloride, liquid amount control 70%, be above mass parts
Number;
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%,
Inoculum concentration 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 20 hours, obtains seed bacterium solution;
C, primary fermentation culture: seed bacterium solution made from b step is imported in (200L) first order seed fermentor, seeding tank
Liquid amount 80%, inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is after 7.5,25 ± 2 DEG C of temperature, culture 30 hours, system
Obtain primary fermentation liquid;
D, primary fermentation liquid made from step c fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
60% liquid amount, inoculum concentration 5%, process pH value control are cultivated 40 hours 7.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed
Afterwards, petroleum hydrocarbon degradation bacterium fermenation raw liquid is made, wherein surfactant hydrocarbon degradation bacteria bacterium number is 2 × 108A/ml or more;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.8 part of liquid paraffin, 2.8 parts of ammonium chloride, potassium dihydrogen phosphate
1.8 parts, 0.8 part of epsom salt, 0.3 part of manganese chloride, 0.4 part of calcium chloride, liquid amount control in 80%, cultivation temperature 40~45
DEG C, blowing air, mixing speed control in 780rpm;
It is above mass fraction, mass percent and mass ratio.
The biopolymer bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation being incubated overnight, fermentation liquid to be mutagenic is made,
Then fermentation liquid to be mutagenic is subjected to 700 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, is centrifuged
Confluent monolayer cells are removed, cell is washed, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C
Overnight incubation dilutes coated plate, and selection can be that carbon source generates xanthan gum using carbohydrate at a temperature of 20~50 DEG C after screening domestication
Strain be target strain;
B, prepared by fermentation liquid:
A, target actication of culture: it is living into the 500ml shaking flask of the fluid nutrient medium containing 100ml that inclined-plane target strain chooses 2 rings
Change culture, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 12 hours, activation bacterium solution is made;
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%,
Inoculum concentration 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 12 hours, obtains seed bacterium solution;
C, one grade fermemtation culture: seed bacterium solution made from b step is imported in (100L) first order seed fermentor, seeding tank
Liquid amount 50%, inoculum concentration 10% control ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is 7.5, temperature 25 ± 2
DEG C, after culture 10 hours, one grade fermemtation bacterium solution is made;
D, one grade fermemtation bacterium solution made from step c second order fermentation culture: is imported into second level (1m3) seeding tank, second level is canned
Liquid measure 50%, inoculum concentration 10% control ventilation quantity 0.55vvm, and 100 revs/min of speed of agitator, pH value control is 7.8, temperature 25 ± 2
DEG C, after culture 10 hours, obtain second order fermentation bacterium solution;
E, second order fermentation bacterium solution made from Step d fermented and cultured: is imported into (10m3) three grade fermemtation tank, three grade fermemtation tank presses
50% liquid amount, inoculum concentration 10%, ventilation quantity 0.75vvm, process pH value control is 7. 5,25 ± 2 DEG C of temperature, mixing speed 80
Rev/min, after culture 8 hours, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermentation life respectively
It produces, after fermentation 18 hours, biopolymer bacterium fermenation raw liquid is made, wherein microbe composite polysaccharide is in microbial fermentation liquid product
In account for 0.8%~1.1%;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup is with sucrose by the mixed liquor of 3:1 quality proportioning
15 parts, 6 parts of yeast extract, 0.25 part of peptone, 1.8 parts of potassium dihydrogen phosphate, 0.4 part of epsom salt, 0.15 part of calcium chloride, dress liquid
At 25 ± 2 DEG C of 30%, cultivation temperature, pH value 7.5, mixing speed is controlled in 100rpm for amount control;
It is above mass fraction, mass percent and mass ratio.
In the application, the one grade fermemtation tank is the tank body that capacity is 100L or 200L;Second order fermentation tank is that capacity is
1m3Tank body;Three grade fermemtation tank is that capacity is 10m3Tank body.
Using petroleum hydrocarbon degradation microbial inoculum obtained by embodiment 1, embodiment 2 or embodiment 3 and biological compound oil displacement agent,
Specific injection technology operating procedure is as follows: by block well injection station, using injection well and extraction well as defined area, by with beam
It speed and requires to inject biological compound oil displacement agent 0.2pv with fluence, then takes over note petroleum hydrocarbon degradation microbial inoculum 0.1pv, take over injection
Biological compound oil displacement agent 0.2pv, continues to inject petroleum hydrocarbon degradation microbial inoculum 0.1pv, finally injects biological compound oil displacement agent 0.2pv,
Finally using water drive instead is to complete a full set of injection technology.
Oil displacement experiment is carried out to a variety of rock cores using injection technology of the invention, experimental result is as follows:
1, oil displacement experiment is carried out to artificial core:
It chooses artificial core and carries out simulation raising recovery ratio experiment, carry out a water drive, water drive moisture content reaches after 98%
On the basis of, multi-element biologic combination flooding medicament is injected by technique, system viscosity control is in 50~70mpas, by calculating,
Recovery ratio situation is as shown in table 1:
The experimental results showed that multi-element biologic combination flooding medicament system averagely improves recovery ratio 21.4% in artificial core.
2, oil displacement experiment is carried out to Berea core:
It chooses Berea core and carries out simulation raising recovery ratio experiment, carry out a water drive, water drive moisture content reaches after 98%
On the basis of, multi-element biologic combination flooding medicament is injected by technique, system viscosity control is in 50~70mpas, by calculating,
Recovery ratio situation is as shown in table 2:
The experimental results showed that multi-element biologic combination flooding medicament system averagely improves recovery ratio 20.9% on Berea core.
3, oil displacement experiment is carried out to natural core:
It chooses natural core and carries out simulation raising recovery ratio experiment, carry out a water drive, water drive moisture content reaches after 98%
On the basis of, multi-element biologic combination flooding medicament is injected by technique, system viscosity control is in 50~70mpas, by calculating,
Recovery ratio situation is as shown in table 3:
The experimental results showed that multi-element biologic combination flooding medicament system averagely improves recovery ratio 18.1% on natural core.
Claims (3)
1. a kind of multi-element biologic compound oil displacement agent system is made of petroleum hydrocarbon degradation microbial inoculum and biological compound oil displacement agent:
The petroleum hydrocarbon degradation microbial inoculum is by 1~5 part of petroleum hydrocarbon degradation bacterium fermenation raw liquid and 15~20 portions of nutrient solutions, mixing
Uniformly it is made;
The biological compound oil displacement agent is made by weight of following component: 5~10 parts of biopolymer bacterium fermenation raw liquid,
10~20 parts of biological lipopeptid surfactant, 1~5 part of glycine betaine, 60~80 parts of water;
The nutrient solution is 0.5~5 part of mixed liquor, the diammonium hydrogen phosphate 0.1 by corn syrup and sucrose by 3:1 quality proportioning
~0.5 part, 0.1~0.5 part of sodium nitrate, 0.02~0.05 part of humic acid sylvite, 60~80 parts of water;
It is above mass fraction.
2. a kind of multi-element biologic compound oil displacement agent system as described in claim 1, it is characterised in that the petroleum hydrocarbon degradation
Bacterium fermenation raw liquid the preparation method is as follows:
A, induction mutation of bacterium: bacillus licheniformis CGMCC1.7461 inoculation being incubated overnight, fermentation liquid to be mutagenic is made, and then will
Fermentation liquid to be mutagenic carries out 600~750 mcg/ml NTG processing, is mixed according to 1:1,25 DEG C of heat preservation 30min, centrifuging and taking
Lower confluent monolayer cells wash cell, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C of trainings
It supports overnight, dilutes coated plate, selection can be single using hexadecane, benzene, phenanthrene and hexamethylene under the conditions of 10 DEG C~25 DEG C of temperature
One carbon source for growth is target strain to the strain that aromatic hydrocarbons and cycloalkane have stronger degradation capability;
B, prepared by fermentation liquid:
A, target actication of culture: inclined-plane target strain chooses 2 rings and activates training into the 500ml shaking flask of the fluid nutrient medium containing 300ml
It supports, 25 ± 2 DEG C, 100 revs/min of temperature shake culture 18~24 hours, activation bacterium solution is made;
B, it prepares seed liquor: shaking flask bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 60%, inoculation
Amount 5% 25 ± 2 DEG C, 100 revs/min of temperature shake culture 24~30 hours, obtains seed bacterium solution;
C, primary fermentation culture: seed bacterium solution made from b step being imported in first order seed fermentor, seeding tank liquid amount 80%,
Inoculum concentration 5%, 100 revs/min of speed of agitator, pH value control is after 7.0~8.0,25 ± 2 DEG C of temperature, culture 24~36 hours, system
Obtain primary fermentation liquid;
D, fermented and cultured: primary fermentation liquid made from step c is imported into three grade fermemtation tank, three grade fermemtation tank is pressed 60% liquid amount, connect
Kind amount 5%, process pH value control is after 7.0~8.5,25 ± 2 DEG C of temperature, 80 revs/min of mixing speed, culture 30~48 hours, system
Obtain petroleum hydrocarbon degradation bacterium fermenation raw liquid;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: 0.5~1 part of liquid paraffin, 2~3 parts of ammonium chloride, potassium dihydrogen phosphate
1.5~2 parts, 0.5~1 part of epsom salt, 0.1~0.5 part of manganese chloride, 0.2~0.5 part of calcium chloride, liquid amount control 60
~80%;
It is above mass fraction, mass percent and mass ratio.
3. a kind of multi-element biologic compound oil displacement agent system as described in claim 1, it is characterised in that the biopolymer
Bacterium fermenation raw liquid is obtained by the following method:
A, induction mutation of bacterium: xanthomonas campestris CGMCC1.1781 inoculation is incubated overnight, fermentation liquid to be mutagenic is made, then
Fermentation liquid to be mutagenic is subjected to 600~750 mcg/ml NTG processing, is mixed, 25 DEG C of heat preservation 30min, is centrifuged according to 1:1
Confluent monolayer cells are removed, cell is washed, are resuspended in LB culture medium, its cell concentration is 1.0 × 108~2.0 × 108A/ml, 25 DEG C
Overnight incubation dilutes coated plate, and selection can be that carbon source generates xanthan gum using carbohydrate at a temperature of 20~50 DEG C after screening domestication
Strain be target strain;
B, prepared by fermentation liquid:
A, target actication of culture: inclined-plane target strain chooses 2 rings and activates training into the 500ml shaking flask of the fluid nutrient medium containing 100ml
It supports, 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, activation bacterium solution is made;
B, it prepares seed liquor: activation bacterium solution made from a step being transferred in 5L shaking flask and is cultivated, 5L shaking flask liquid amount 20%, inoculation
Amount 10% 25 ± 2 DEG C, 150 revs/min of temperature shake culture 10~15 hours, obtains seed bacterium solution;
C, one grade fermemtation culture: seed bacterium solution made from b step being imported in first order seed fermentor, seeding tank liquid amount 50%,
Inoculum concentration 10% controls ventilation quantity 0.5vvm, and 130 revs/min of speed of agitator, pH value control is trained 7.0~8.0,25 ± 2 DEG C of temperature
After supporting 8~13 hours, one grade fermemtation bacterium solution is made;
D, second order fermentation culture: one grade fermemtation bacterium solution made from step c is imported into secondary seed tank, the canned liquid measure 50% of second level connects
Kind of amount 10%, controls 0.5~0.6vvm of ventilation quantity, 100 revs/min of speed of agitator, pH value control is 7.0~8.0, temperature 25 ± 2
DEG C, after culture 8~13 hours, obtain second order fermentation bacterium solution;
E, fermented and cultured: importing three grade fermemtation tank for second order fermentation bacterium solution made from Step d, and three grade fermemtation tank presses 50% liquid amount,
Inoculum concentration 10%, 0.7~0.8vvm of ventilation quantity, process pH value control 7.0~8.5,25 ± 2 DEG C of temperature, 80 turns of mixing speed/
Point, after culture 7~10 hours, fermentation liquid is imported and presses same process parameter in same volume fermentor, continues fermentation life respectively
It produces, after fermentation 15~20 hours, biopolymer bacterium fermenation raw liquid is made;
Above-mentioned shaking flask and fermentation tank culture based formulas at different levels are: corn syrup and sucrose by 3:1 quality proportioning mixed liquor 10~
20 parts, 5~7 parts of yeast extract, 0.2~0.3 part of peptone, 1.5~2 parts of potassium dihydrogen phosphate, 0.3~0.5 part of epsom salt, chlorine
Change 0.1~0.2 part of calcium, liquid amount control at 25 ± 2 DEG C of 20~50%, cultivation temperature, pH value 7~8, mixing speed is controlled 80
~150rpm;
It is above mass fraction, mass percent and mass ratio.
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CN109897621A (en) * | 2019-03-25 | 2019-06-18 | 大庆华理生物技术有限公司 | A kind of binary biology oil displacement agent and its application |
CN110452866A (en) * | 2019-08-26 | 2019-11-15 | 北京大学 | A kind of activator that orientation activates core microorganism and its application in microbe oil production |
CN114426834A (en) * | 2020-10-14 | 2022-05-03 | 中国石油化工股份有限公司 | Biological aerosol system and preparation method and application thereof |
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