CN107311313A - A kind of composite bacteria fermentation improver of water quality used for aquiculture - Google Patents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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Abstract
The present invention relates to a kind of improver of water quality, and in particular to a kind of composite bacteria fermentation improver of water quality used for aquiculture, the modifying agent includes following raw material：Zymotic fluid, inorganic salts, wheat bran, Cottonseed Meal, brewer's wort and trace element, inorganic salts are the inorganic salts containing potassium, magnesium, ammonium, sodium and calcium, trace element is the inorganic salts containing manganese, aluminium, copper, boron, molybdenum and zinc, liquid medium starting material is added in fermentation tank and sterilized, when sterilizing finishes after fermentation tank temperature and is down to 30 DEG C, add fermented by mixed bacterium and produce zymotic fluid, trace element and inorganic salts are mixed to material A of being sieved to obtain after dry, pulverize；Wheat bran and Cottonseed Meal are added in brewer's wort after drying and crushing sieving, material B is mixed to obtain；A, B fermentation liquid are mixed and produce improver of water quality, improver of water quality of the invention can regulating water quality, merged a variety of floras, improved substrate, decomposed the organic matter such as residual bait, excrement in pond, have no toxic side effect, it is pollution-free, with preferable application value.
Description
Technical field
The invention belongs to aquaculture water quality improving technology field, and in particular to a kind of composite bacteria fermentation used for aquiculture
Improver of water quality.
Background technology
The rise of China's culture fishery, eats fish hardly possible to solving China resident, alleviates China's marine fishery resources increasingly deficient
Weary and protection marine fishery resources has all played great function, and solves Employment problem, generates huge economy
Benefit and social benefit.With culture fishery fast development, breeding ecological environment is destroyed.Quality problem is restriction aquatic products
Cultivate the key factor of sustainable development.The excreta of the feed, fertilizer and the cultivated animals that are put into Cultivated water is largely accumulated
It is tired, water quality deterioration can be made, cause dissolved oxygen to decline, the harmful substances such as ammonia nitrogen, nitrite are produced；Harmful bacteria grows, and cultivation is dynamic
Thing disease takes place frequently.Meanwhile, harmful substance also results in the growth of cultivated animals by extreme influence, makes its edible nutritive value
Have a greatly reduced quality.So, usual seedling pond must just fall pond for 7-10 days, but periodically fall pond and can bring another periodic problem, pond
In the water quality newly changed it is generally barrenr, there is larger difference with changing the environment before water, what this had resulted in cultivated animals stress
Reaction.Stress likewise, the water quality of cultivating pool is larger because the change of environment can also be produced to pond life.Therefore, in time
Regulate and control water quality, the problem of increase cultivation success rate turns into culture fishery sustainable development.
The content of the invention
In order to solve the above technical problems, the invention provides a kind of composite bacteria fermentation improver of water quality used for aquiculture,
It can change water quality barren state, strength activation and nutrition water environment rapidly, improve Tiny ecosystem regulating effect.
To achieve these goals, the present invention is achieved by the following technical programs：
A kind of composite bacteria fermentation improver of water quality used for aquiculture, includes the raw material of following parts by weight：Zymotic fluid 20~
33 parts, 1~5 part of inorganic salts, 5~10 parts of wheat bran, 6~15 parts of Cottonseed Meal, 3~7 parts of brewer's wort, trace element 0.5~2 part；
The zymotic fluid is made up of phaffiafhodozyma, white-rot fungi, soft rotten fungi, lactic acid bacteria, Rhodopseudomonas palustris
Mixed bacteria liquid medium within ferment it is obtained；
It is preferred that, the composite bacteria fermentation improver of water quality used for aquiculture includes the raw material of following parts by weight：Fermentation
26 parts of liquid, 3 parts of inorganic salts, 6 parts of wheat bran, 10 parts of Cottonseed Meal, 5 parts of brewer's wort, 0.9 part of trace element；
It is preferred that, the preparation method of the zymotic fluid is：
Fluid nutrient medium is added in fermentation tank, 115 DEG C of sterilizing 20min, subject to sterilization to finish, fermentation jar temperature is down to 30
DEG C when, by fluid nutrient medium:Mixed bacteria=1000:1 mass ratio adds mixed bacteria, ferment 50 at 25~33 DEG C~
72h, it is 5 × 10 that strain content is made after the completion of fermentation12Cfu/ml zymotic fluid；
The mass ratio of the mixed bacteria is：Phaffiafhodozyma:White-rot fungi:Soft rotten fungi:Lactic acid bacteria:Marsh is red false single
Born of the same parents bacterium=7:3:2:5:1
The fluid nutrient medium, includes the raw material of following percentage by weight：Glucose 10~15%, yeast extract 5~10%,
Peptone 5~10%, salt 8~12%, water 50~65%；
It is preferred that, the inorganic salts are the one or more in the inorganic salts containing potassium, magnesium, ammonium, sodium and calcium；
It is preferred that, the trace element is containing the one or more in manganese, aluminium, copper, boron, molybdenum and zinc inorganic salts；
The preparation method of above-mentioned improver of water quality, step is as follows：
(1) trace element and inorganic salts are weighed by weight and are well mixed, 5~13h is dried at 30~40 DEG C, are crushed
After sieve to obtain material A；
(2) wheat bran and Cottonseed Meal are dried into 10~20h under normal temperature condition, are added to after pulverizing and sieving in brewer's wort, mixed
Conjunction uniform stirring is uniform to obtain material B；
(3) zymotic fluid of material A, B and formula ratio is well mixed, produces above-mentioned improver of water quality.
Beneficial effects of the present invention are：
1st, the present invention has merged a variety of floras, wherein, phaffiafhodozyma can produce the carotenoid such as astaxanthin, in water
The improver of water quality of astaxanthin-containing can make salmon, the skin of trout, muscle as wild fish in bright-coloured red in production aquaculture
Color, can increase the resistance against diseases of fish, improve the fertility of fish, and then produce higher economic benefit.Meanwhile, saccharomycete is preferably
Aerobic microorganism, with very strong fermentability, during the fermentation, multiple beneficial bacterium breeds rapidly, produces a large amount of beneficial generations
Thank to thing, secret out of various active catabolic enzyme, these enzymes have very strong catalytic decomposition ability.Weeds, leaf etc., which can be decomposed, to be had
Machine thing formation humus；The mineral matters such as shale can be decomposed again, produce the active material that can be absorbed and used by plants；It can also degrade
The chemical compositions such as chemical fertilizer, agricultural chemicals.
2nd, the laccase that white-rot fungi, soft rotten fungi fermentation are produced is the polyphenol oxidase of cupric, with good stability,
Can be degraded the lignin such as rotten wood and the various phenols of oxidation and aminated compounds, and then reduce the plain material of water intoxication.
3rd, Rhodopseudomonas palustris can be carried out photosynthetic using light as the energy using the organic matter in environment, hydrogen sulfide, ammonia etc.
Effect, so that fixed nitrogen, dehydrogenation, carbon sequestration, nutrition purify water, while having good breeding environment adaptability.
To sum up, composite fermentation organic matter improver of water quality used for aquiculture of the invention can regulating water quality, merged many
Flora is planted, improves substrate, the organic matter such as residual bait, excrement in pond is decomposed, regulates and controls the micro-ecological environment of breeding environment, improve aquatic
The nutritive value and survival rate of animal, and have no toxic side effect, it is pollution-free, with preferable application value.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with specific embodiment pair
The present invention is described in detail.It should be pointed out that not making creative work premise to those of ordinary skill in the art
Lower obtained every other embodiment, belongs to the scope of protection of the invention.
Embodiment 1：
A kind of composite bacteria fermentation improver of water quality used for aquiculture, includes the raw material of following parts by weight：25 parts of zymotic fluid,
K2HPO41 part, MgSO41.5 parts, (NH)2SO40.5 part, 5 parts of wheat bran, 8 parts of Cottonseed Meal, 3 parts of brewer's wort, MnSO40.2 part,
AlCl30.3 part, CuCl20.1 part, H3BO40.4 part；
The preparation method of above-mentioned zymotic fluid comprises the following steps：
(1) preparation of fluid nutrient medium：Each component raw material is weighed by following percentage composition：Glucose 12%, yeast extract
8%th, peptone 6%, salt 9%, water 55%, fully mix and produce fluid nutrient medium；
(2) ferment：Aforesaid liquid culture medium is added in fermentation tank, 115 DEG C of sterilizing 20min, subject to sterilization to finish, fermentation
When tank temperature is down to 30 DEG C, by fluid nutrient medium:Mixed bacteria=1000:1 mass ratio adds mixed bacteria, is issued at 25 DEG C
Ferment 58h, it is 5 × 10 that strain content is made after the completion of fermentation12Cfu/ml zymotic fluid；
The mixed bacteria includes phaffiafhodozyma, white-rot fungi, soft rotten fungi, lactic acid bacteria, Rhodopseudomonas palustris；
The mass ratio of the mixed bacteria is：Phaffiafhodozyma:White-rot fungi:Soft rotten fungi:Lactic acid bacteria:Marsh is red false single
Born of the same parents bacterium=7:3:2:5:1；
The preparation method of above-mentioned improver of water quality, step is as follows：
(1) K is weighed by weight2HPO4、MgSO4、(NH)2SO4、MnSO4、AlCl3、CuCl2、H3BO4, done at 35 DEG C
Dry 8h, crosses 100 mesh sieves and obtains material A after crushing；
(2) wheat bran and Cottonseed Meal are dried into 17h under normal temperature condition, were added to after crushing 100 mesh sieves in brewer's wort, mixed
Conjunction stirs to obtain material B；
(3) zymotic fluid of material A, B and formula ratio is well mixed, produces above-mentioned improver of water quality.
Embodiment 2：
A kind of composite bacteria fermentation improver of water quality used for aquiculture, it is characterised in that：Include the raw material of following parts by weight：
20 parts of zymotic fluid, (NH)2SO40.6 part, KH2PO40.2 part, 0.3 part of NaCl, CaCl20.9 part, 10 parts of wheat bran, Cottonseed Meal 15
Part, 6 parts of brewer's wort, AlCl30.3 part, CuCl20.4 part, H3BO40.1 part, NaMoO40.1 part；
The preparation method of above-mentioned zymotic fluid comprises the following steps：
(1) preparation of fluid nutrient medium：Each component raw material is weighed by following percentage composition：Glucose 10%, yeast extract
6%th, peptone 5%, salt 12%, water 65%, fully mix and produce fluid nutrient medium；
(2) ferment：Aforesaid liquid culture medium is added in fermentation tank, 115 DEG C of sterilizing 20min, subject to sterilization to finish, fermentation
When tank temperature is down to 30 DEG C, by fluid nutrient medium:Mixed bacteria=1000:1 mass ratio adds mixed bacteria, is issued at 30 DEG C
Ferment 72h, produces above-mentioned zymotic fluid；
The mixed bacteria includes phaffiafhodozyma, white-rot fungi, soft rotten fungi, lactic acid bacteria, Rhodopseudomonas palustris；
The mass ratio of the mixed bacteria is：Phaffiafhodozyma:White-rot fungi:Soft rotten fungi:Lactic acid bacteria:Marsh is red false single
Born of the same parents bacterium=7:3:2:5:1；
The preparation method of above-mentioned improver of water quality, step is as follows：
(1) weigh by weight (NH)2SO4、KH2PO4、NaCl、CaCl2、AlCl3、CuCl2、H3BO4、NaMoO4, 30
10h is dried at DEG C, 200 mesh sieves are crossed after crushing and obtain material A；
(2) wheat bran and Cottonseed Meal are dried into 17h under normal temperature condition, were added to after crushing 200 mesh sieves in brewer's wort, mixed
Conjunction stirs to obtain material B；
(3) zymotic fluid of material A, B and formula ratio is well mixed, produces above-mentioned improver of water quality.
Embodiment 3：
A kind of composite bacteria fermentation improver of water quality used for aquiculture, it is characterised in that：Include the raw material of following parts by weight：
29 parts of zymotic fluid, (NH)2SO41 part, KH2PO42 parts, 1 part of NaCl, CaCl21 part, 7 parts of wheat bran, 6 parts of Cottonseed Meal, brewer's wort 7
Part, MnSO40.05 part, AlCl30.05 part, CuCl20.1 part, H3BO40.1 part, NaMoO40.1 part；
The preparation method be the same as Example 1 of above-mentioned zymotic fluid；
The preparation method be the same as Example 1 of above-mentioned improver of water quality.
Embodiment 4：
A kind of composite bacteria fermentation improver of water quality used for aquiculture, it is characterised in that：Include the raw material of following parts by weight：
Phaffiafhodozyma, white-rot fungi, soft rotten fungi, lactic acid bacteria, Rhodopseudomonas palustris fermentation well mixed 33 parts of liquid, (NH)2SO4
0.3 part, KH2PO40.1 part, 0.1 part of NaCl, CaCl20.5 part, 8 parts of molasses, 9 parts of wheat bran, 4 parts of yeast extract, 5 parts of peptone,
4 parts of brewer's wort, AlCl30.05 part, CuCl20.03 part, H3BO40.05 part, NaMoO40.05 part, ZnSO40.02 part；
The preparation method be the same as Example 2 of above-mentioned zymotic fluid；
The preparation method be the same as Example 2 of above-mentioned improver of water quality.
Embodiment 5：
A kind of composite bacteria fermentation improver of water quality used for aquiculture, includes the raw material of following parts by weight：Phaffiafhodozyma,
White-rot fungi, soft rotten fungi, lactic acid bacteria, 26 parts of Rhodopseudomonas palustris zymotic fluid, K2HPO40.5 part, MgSO40.5 part,
(NH)2SO40.3 part, KH2PO40.3 part, 0.7 part of NaCl, CaCl20.7 part, 6 parts of wheat bran, 10 parts of Cottonseed Meal, brewer's wort 5
Part, MnSO40.2 part, AlCl30.1 part, CuCl20.3 part, H3BO40.1 part, NaMoO40.1 part, ZnSO40.1 part；
The preparation method be the same as Example 1 of above-mentioned zymotic fluid；
The preparation method be the same as Example 2 of above-mentioned improver of water quality.
Application experiment result：
Test influence of 1 product to fish seedling percent：
Experiment is divided into 5 groups of test groups and 1 group of control group, and every group of aquaculture area is identical, i.e., every mu is put in a suitable place to breed 3000 salmons
Fish, improver of water quality made from the test group introducing embodiment of the present invention 1~5 is raised, and control group introduces what is bought from market
Improver of water quality is raised, and puts the latter every mu improver of water quality for spilling 2.5kg in a suitable place to breed, is fished for 1000 fishes at random after 60 days and is seen
Examine.
It is as shown in the table for the influence experimental result of salmon survival rate：
The influence experimental result of the salmon shoot survival percent of table 1
By experimental data as can be seen that using the survival rate average out to 97.5% of the improver of water quality fry of the present invention,
20.3% is higher by than control group, the survival rate of fry is substantially increased, fry is after raising after a while, test group seedling body
Length is longer than control group, and the skin of test group fry is in bright-coloured red, and the skin of control group fry is in silver gray, is said
Bright improver of water quality of the invention can also promote the growth of content astaxanthin in fry body, with higher nutritive value.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those within the art that：It still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (7)
1. a kind of composite bacteria fermentation improver of water quality used for aquiculture, it is characterised in that：Include the raw material of following parts by weight：Hair
20~33 parts of zymotic fluid, 1~5 part of inorganic salts, 5~10 parts of wheat bran, 6~15 parts of Cottonseed Meal, 3~7 parts of brewer's wort, trace element 0.5
~2 parts；
The zymotic fluid is by mixing that phaffiafhodozyma, white-rot fungi, soft rotten fungi, lactic acid bacteria, Rhodopseudomonas palustris are constituted
Close made from being fermented in strain liquid medium within.
2. composite bacteria fermentation improver of water quality used for aquiculture according to claim 1, it is characterised in that：Including following
The raw material of parts by weight：26 parts of zymotic fluid, 3 parts of inorganic salts, 6 parts of wheat bran, 10 parts of Cottonseed Meal, 5 parts of brewer's wort, 0.9 part of trace element.
3. composite bacteria fermentation improver of water quality used for aquiculture according to claim 1, it is characterised in that：The mixing
The mass ratio of strain is：Phaffiafhodozyma:White-rot fungi:Soft rotten fungi:Lactic acid bacteria:Rhodopseudomonas palustris=7:3:2:5:1.
4. composite bacteria fermentation improver of water quality used for aquiculture according to claim 1, it is characterised in that：The fermentation
The preparation method of liquid is：
Fluid nutrient medium is added in fermentation tank, 115 DEG C of sterilizing 20min, subject to sterilization to finish, fermentation jar temperature is down to 30 DEG C
When, by fluid nutrient medium:Mixed bacteria=1000:1 mass ratio adds mixed bacteria, and ferment 50~72h at 25~33 DEG C,
It is 5 × 10 that strain content is made after the completion of fermentation12Cfu/ml zymotic fluid；
The fluid nutrient medium, includes the raw material of following percentage by weight：Glucose 10~15%, yeast extract 5~10%, albumen
Peptone 5~10%, salt 8~12%, water 50~65%.
5. composite bacteria fermentation improver of water quality used for aquiculture according to claim 1, it is characterised in that：It is described inorganic
Salt is the one or more in the inorganic salts containing potassium, magnesium, ammonium, sodium and calcium.
6. composite bacteria fermentation improver of water quality used for aquiculture according to claim 1, it is characterised in that：It is described micro
Element is the one or more in the inorganic salts containing manganese, aluminium, copper, boron, molybdenum and zinc.
7. the preparation method for the improver of water quality that fermented according to the composite bacteria used for aquiculture described in claim any one of 1-6, its
It is characterised by：Comprise the following steps：
(1) trace element and inorganic salts are weighed by weight and are well mixed, and 5~13h, mistake after crushing are dried at 30~40 DEG C
Sieve to obtain material A；
(2) wheat bran and Cottonseed Meal are dried into 10~20h under normal temperature condition, are added to after pulverizing and sieving in brewer's wort, mixing is stirred
Mix uniform material B；
(3) zymotic fluid of material A, B and formula ratio is well mixed, produces above-mentioned improver of water quality.
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