CN107303538B - A kind of biology molecule separating equipment and separation method - Google Patents
A kind of biology molecule separating equipment and separation method Download PDFInfo
- Publication number
- CN107303538B CN107303538B CN201710376343.1A CN201710376343A CN107303538B CN 107303538 B CN107303538 B CN 107303538B CN 201710376343 A CN201710376343 A CN 201710376343A CN 107303538 B CN107303538 B CN 107303538B
- Authority
- CN
- China
- Prior art keywords
- injection apparatus
- separation
- electric field
- sample
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C7/00—Separating solids from solids by electrostatic effect
- B03C7/003—Pretreatment of the solids prior to electrostatic separation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C7/00—Separating solids from solids by electrostatic effect
- B03C7/02—Separators
- B03C7/12—Separators with material falling free
Abstract
The invention discloses a kind of biology molecule separating equipment and separation methods, biology molecule separating equipment of the present invention is the injection apparatus for including at least one receiving sample, injection apparatus is oppositely arranged with electrode, and electrode surface is arc or inclined-plane, and injection apparatus and electrode connect to power supply.Bio-molecular separation method of the present invention is the following steps are included: be sample to be separated in a, injection apparatus, different biological molecules, to outlet end motion, realize biomolecule pre-separation under the action of electric field；B, different biological molecules are sprayed with friction speed from injection apparatus, into electric field, further separation in the electric field；C, for different biological molecules by colliding after electric field with electrode surface, biomolecule does parabolic motion to side with different initial velocity, realizes sample separation.The present invention can efficiently, precisely realize bio-molecular separation, obtain purity is high, the single sample of molecular composition, meet scientific research needs, can be in field of biomedicine application.
Description
Technical field
The present invention relates to separator and separation method, in particular to a kind of biology molecule separating equipment and separation method.
Background technique
The development of life science need to be to the research of biomolecule, however the research material generally obtained is all mixed biology
Molecule obtains congener biomolecule, congener biomolecule difference point even if separating to mixed biomolecule
The separation of son amount is very cumbersome and separating effect is poor, is unable to satisfy particular demands.In the prior art to mixed biologic molecule point
Mainly there are capillary electrophoresis technique (CE) and Multidimensional HPLC technology from method.It with capillary is point that CE technology, which is a kind of,
From channel, using high-voltage dc as the liquid phase separation analytical technology of driving force, capillary electrophoresis technique is limited to treating capacity, more
For detecting, sample can not be separated and collected, need to be used in conjunction with mass spectrograph, but still can not get rid of such technology itself
Defect, such as poor reproducibility, sensitivity are low.Multidimensional HPLC, i.e., using two or more chromatographic columns to complicated sample
Component in product is separated, and will pass through all or part of component selection separated for the first time using switching valve between column and column
Be transferred in one or more another different types of chromatographic column and be further separated, use the instrument of Multidimensional HPLC method
Because its internal pressure is big, service life is relatively short, and both the above method use cost is high, and analysis time is long.
Summary of the invention
Goal of the invention: it is an object of the present invention to provide a kind of device for bio-molecular separation, which can be efficient
Separation, purifying and collection biomolecule.It is a further object of the present invention to provide the bio-molecular separation methods based on the device.
Technical solution: a kind of biology molecule separating equipment of the present invention accommodates the injection dress of sample including at least one
It sets, injection apparatus is oppositely arranged with electrode, and electrode surface is arc or inclined-plane, and injection apparatus and electrode are connected with power supply.Electrode
When surface is arc, radian is controllable, to realize different reflection angles, reaches different separation purposes.Injection apparatus is diameter
For 0.01-5 microns of tubular structure, outlet diameter is 5-500 nanometers.For improve separative efficiency, settable multiple injection apparatus,
Device is arranged in parallel or Circle-Array column arranges.
Electrolyte solution is filled in injection apparatus, when electrolyte solution powers on for separator, as conducting medium.
A kind of separation method based on above-mentioned separator of the present invention, comprising the following steps: be in a, injection apparatus
Sample to be separated, separator power on, and the different biological molecules in sample to be separated are under the action of electric field to outlet end
Biomolecule pre-separation is realized in movement；B, different biological molecules are sprayed with friction speed from injection apparatus, into electric field, in electricity
It is further separated in；C, different biological molecules are after electric field with electrode surface by colliding, biomolecule with it is different just
Speed does parabolic motion to side, and different biological molecules drop point is different, realizes sample separation.
According to different separation samples, the injection apparatus of suitable diameter is selected, it is accurate to the separation of biomolecule to improve
Degree.Injection apparatus outlet diameter is 5-500 nanometers, can directly filter out the excessive biomolecule of diameter, further increase separation
Accuracy can not be isolated at all particularly with the biomolecule of some small-molecular-weights by common separation method, or
It need to be separated by complicated means, process is tedious, and cost is very high, and the effect is unsatisfactory.The present invention passes through biology
Difference between the carried charge or quality of molecule, certain speed is made it have by injection apparatus, is passing through electric field into one
Step separation, expands intermolecular difference, after colliding with electrode, does parabolic motion, further expansion different biological molecules it
Between difference, to realize the accurate separation of low molecular weight biomolecule.In addition, when electrode surface is arc, it can be according to not
Same separation purpose is designed the size of radian, increases the effect of bio-molecular separation.
Different biological molecules of the present invention include different types of biomolecule, also include the different similar life of molecular weight
Object molecule.
The utility model has the advantages that the disposable purifies and separates of different biological molecules can be realized, without being combined with other equipment, reduce
Disengaging time and step, isolated biomolecule purity is high, precision are high.The diameter of injection apparatus in the present invention is controllable
, the injection apparatus of suitable diameter can be selected according to different biological molecules solution, improve preciseness of separation.
Detailed description of the invention
Fig. 1 is biology molecule separating equipment structural schematic diagram；
Fig. 2 is that Circle-Array column arranges injection apparatus structural schematic diagram.
Specific embodiment
Referring to Fig. 1, biology molecule separating equipment of the invention includes an injection apparatus 1, and injection apparatus 1 is by a diameter
It is formed for 2 microns of tubular structures, tubular structure outlet end diameter is 20 nanometers, is filled with electrolyte solution in tubular structure.
Certainly, the diameter of injection apparatus tubular structure can choose any range in 0.01-5 microns, and outlet diameter can be in 5-500
Any range is chosen in nanometer.Be sample 2 to be separated inside injection apparatus 1, injection apparatus 1 is oppositely arranged with electrode 3, the two it
Between it is at regular intervals.Electrode surface is arc, and injection apparatus 1 is connected with electrode 3 and power supply 4.
Referring to fig. 2, biology molecule separating equipment of the invention, including the injection apparatus arranged by four Circle-Array column,
The same Fig. 1 in part is not drawn into Fig. 2.
Using biology molecule separating equipment shown in FIG. 1, sample 2 to be separated is separated, electrolyte is filled in injection apparatus 1
Solution, electrolyte solution are selected according to different separation samples, connect separator power supply 4, under the action of electric field, wait divide
Start to export end motion to injection apparatus from the different biological molecules in sample 2, sample pre-separation in injection apparatus, then,
Different biological molecules, which are sprayed with friction speed from injection apparatus 1, enters electric field, and further separation, biomolecule pass through in the electric field
It collides after electric field with 3 surface of electrode, different biological molecules start to change the direction of motion, are thrown with different initial velocity to side
The movement of object line, eventually falls into the collector of different location, different biological molecules is separated, and collects.
Claims (3)
1. a kind of biology molecule separating equipment, it is characterised in that: accommodate the injection apparatus (1) of sample, injection including at least one
Device (1) is oppositely arranged with electrode (3), and electrode (3) surface is arc or inclined-plane, and injection apparatus (1) and electrode (3) are separately connected
At the both ends of power supply (4)；The injection apparatus (1) is the tubular structure that diameter is 0.01-5 microns, and the injection apparatus (1) goes out
Mouth diameter is 5-500 nanometers, is filled with electrolyte solution in the injection apparatus (1).
2. biology molecule separating equipment according to claim 1, which is characterized in that the injection apparatus (1) is parallel
Column or the arrangement of Circle-Array column.
3. a kind of separation method based on separator described in claim 1, which comprises the following steps:
It a, is sample to be separated (2) that separator powers on (4), the difference in sample (2) to be separated in injection apparatus (1)
Biomolecule, to outlet end motion, realizes biomolecule pre-separation under the action of electric field；
B, different biological molecules are sprayed with friction speed from injection apparatus (1), into electric field, further separation in the electric field；
C, different biological molecules are after electric field with electrode (3) surface by colliding, and biomolecule is with different initial velocity to one
Parabolic motion is done in side, and different biological molecules drop point is different, realizes sample separation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710376343.1A CN107303538B (en) | 2017-05-23 | 2017-05-23 | A kind of biology molecule separating equipment and separation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710376343.1A CN107303538B (en) | 2017-05-23 | 2017-05-23 | A kind of biology molecule separating equipment and separation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107303538A CN107303538A (en) | 2017-10-31 |
| CN107303538B true CN107303538B (en) | 2019-05-31 |
Family
ID=60150912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710376343.1A Active CN107303538B (en) | 2017-05-23 | 2017-05-23 | A kind of biology molecule separating equipment and separation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107303538B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109225642B (en) * | 2018-10-16 | 2019-09-03 | 江南大学 | A kind of superfine powder wet electrostatic grading plant |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991000915A1 (en) * | 1989-07-11 | 1991-01-24 | Biotechnology Research & Development Corporation | Aerosol beam microinjector |
| US20020150669A1 (en) * | 1997-06-12 | 2002-10-17 | Regents Of The University Of Minnesota | Electrospraying apparatus and method for coating particles |
| CN1539012A (en) * | 2001-08-03 | 2004-10-20 | ���С�����˹�Ƽ���˾ | Electrodes coated with treating agent and uses thereof |
| CN101462094A (en) * | 2007-12-18 | 2009-06-24 | 杨卫华 | Injection type electrostatic sorting method and apparatus |
| CN103189143A (en) * | 2010-07-08 | 2013-07-03 | 赢创德固赛有限公司 | Electric sorting by means of corona discharge |
| CN103718020A (en) * | 2012-03-30 | 2014-04-09 | 索尼公司 | Microparticle fractionation apparatus, and method for optimizing fluid stream in said apparatus |
| CN104785164A (en) * | 2015-04-15 | 2015-07-22 | 西北大学 | Device for preparing colloidal particles through electrostatic spraying and control method of device |
-
2017
- 2017-05-23 CN CN201710376343.1A patent/CN107303538B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991000915A1 (en) * | 1989-07-11 | 1991-01-24 | Biotechnology Research & Development Corporation | Aerosol beam microinjector |
| US20020150669A1 (en) * | 1997-06-12 | 2002-10-17 | Regents Of The University Of Minnesota | Electrospraying apparatus and method for coating particles |
| CN1539012A (en) * | 2001-08-03 | 2004-10-20 | ���С�����˹�Ƽ���˾ | Electrodes coated with treating agent and uses thereof |
| CN101462094A (en) * | 2007-12-18 | 2009-06-24 | 杨卫华 | Injection type electrostatic sorting method and apparatus |
| CN103189143A (en) * | 2010-07-08 | 2013-07-03 | 赢创德固赛有限公司 | Electric sorting by means of corona discharge |
| CN103718020A (en) * | 2012-03-30 | 2014-04-09 | 索尼公司 | Microparticle fractionation apparatus, and method for optimizing fluid stream in said apparatus |
| CN104785164A (en) * | 2015-04-15 | 2015-07-22 | 西北大学 | Device for preparing colloidal particles through electrostatic spraying and control method of device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107303538A (en) | 2017-10-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7811438B2 (en) | Bio-enrichment device to enhance sample collection and detection | |
| Lin et al. | Sample preconcentration in microfluidic devices | |
| Sharma et al. | Hand-portable liquid chromatographic instrumentation | |
| Swartz et al. | Ultra performance liquid chromatography: Tomorrow’s HPLC technology today | |
| CN109030689B (en) | Capillary chromatographic column pre-assembling preparation method | |
| CN102527454A (en) | Micro-fluid control drop concentration device for sample enrichment | |
| CN102628870B (en) | Micro-nanofluidic chip and method for achieving rapid fluorescent labeling of proteins | |
| CN107303538B (en) | A kind of biology molecule separating equipment and separation method | |
| Morani et al. | Recent electrokinetic strategies for isolation, enrichment and separation of extracellular vesicles | |
| CN109331893A (en) | Micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus | |
| Rezvani et al. | Polyamide/titania hollow nanofibers prepared by core–shell electrospinning as a microextractive phase in a fabricated sandwiched format microfluidic device | |
| WO2021239010A1 (en) | Cell sorting chip, apparatus, and method based on dielectric deterministic displacement | |
| CN102824854A (en) | Electrophoresis apparatus and its application | |
| CN201735407U (en) | Microfluidic control device for continuous high-throughput phosphopeptide enrichment and separation | |
| CN102565255B (en) | PCEC (Pressurized Capillary Electrochromatography) ELSD (Evaporative Light-Scattering Detector) | |
| CN205175977U (en) | Take high -efficient gas chromatograph of antifouling device | |
| CN101813584A (en) | Gas flow type dynamic liquid phase micro-extraction method | |
| CN105879851B (en) | Graphite oxide ene-type in-line purification Solid Phase Extraction integral post and preparation method and purposes | |
| Ma et al. | New analytical spiral tube assembly for separation of proteins by counter-current chromatography | |
| Adam et al. | Nanomaterials for sample pretreatment prior to capillary electrophoretic analysis | |
| CN205484222U (en) | Liquid chromatograph improves device | |
| CN104359995A (en) | Method for separating bio-macromolecules of flow type stationary phase in column by utilizing electromagnetic field | |
| CN104056468B (en) | Without the need to electrofocusing's method of the separation amphiprotic substance of ampholytes | |
| Liu et al. | Separation of aromatic acids by wide‐bore electrophoresis with nanoparticles prepared by electrospray as pseudostationary phase | |
| Ebrahimi et al. | Molecular separation by using active and passive microfluidic chip designs: a comprehensive review |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |