CN107299064A - One plant of marine actinomycete S 19 3 with broad spectrum antibiotic activity - Google Patents
One plant of marine actinomycete S 19 3 with broad spectrum antibiotic activity Download PDFInfo
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Abstract
The present invention is one plant of marine actinomycete S 19 3 with broad spectrum antibiotic activity, is isolated from the surface deposit of Jiaozhou Bay observation erect-position collection.Actinomyces S 19 3 bacteriostatic activity is detected using Odontothrips loti and disk diffusion method, as a result shows that it is folded micrococcus luteus, Escherichia coli, pseudomonas aeruginosa, Candida albicans to gamboge eight and is respectively provided with good fungistatic effect.Identify that Preliminary Identification marine actinomycete S 19 3 is small pseudomonas bacillus (Micromonospora lupini) by carrying out the polyphase sort such as morphological feature and physiological and biochemical property observation, 16S rDNA sequencings and phylogenetic analysis to marine actinomycete S 19 3.Successful amplification goes out I type polyketide synthase (PKS I) gene from marine actinomycete S 19 3 genome, indicates that it has the potential ability of production polyketide.
Description
Technical field
The present invention relates to the marine actinomycete S-19-3 that one plant has broad spectrum antibiotic activity, it belongs to unwrapping wire in microbiology
Bacterium field.
Background technology
Actinomyces (Actinomycetes) be high (G+C) content of a class (>55%) gram-positive bacterium, because of its bacterium
Fall edge mycelia and often gain the name [1] in unwrapping wire shape.Actinomyces possess what synthesis was enriched as the valuable microbial resources of a class
The ability of the secondary metabolite of bioactivity.At present in 33500 kinds of natural active products having found, more than 40% is putting
[2] are found in line bacterium；Commonly used all kinds of antibiotic about 70% are also to be produced [3] by actinomyces in life.
Marine environment is created due to its special far (high pressure, low temperature, high salt, shortage illumination, poor nutritional etc.)
The unique life style of marine microorganism, metabolic pathway, so as to possess the ability [4] of synthesis novel active compound so that sea
Foreign microorganism becomes one of important sources of bioactive natural product, main force [5] also as new drug development.According to statistics, come
The reactive compound for coming from actinomyces accounts for the 45% of microorganism natural active product, and these structures are novel, vdiverse in function and have
There are discovery and its application value potentiality contained of the marine actinomycete secondary metabolite of special physiological activity, be the micro- life in ocean
The exploitation of the natural products in thing source is laid the foundation, and scientific basis is provided for efficient utilize of Marine Microorganisms.
[1] girth woods, microbiology, Beijing：China Medical Science Press, 2009
[2]Bérdy J.Thoughts and facts about antibiotics:where we are now and
where we are heading.Journal of Antibiotics,2012,65(8):385~395
[3]Bérdy J.Bioactive microbial metabolites.Journal of Antibiotics,
2005,58(1):1~26
[4] Zhao Chengying, Zhu Tonghan, Zhu Weiming, 2010~2013 marine microorganism new natural product, organic chemistry
2013,33 (6)：1195-1234
[5]Hu G P,Yuan J,Sun L,et al.Statistical research on marine natural
products based on data obtained between 1985and 2008.Marine Drugs,2011,9(4):
514~525
The content of the invention
In view of above-mentioned, there is provided the sea that one plant has broad spectrum antibiotic activity using Jiaozhou Bay's Marine Microorganisms by the present invention
Foreign actinomyces, particular technique is as follows：
One plant of marine actinomycete S-19-3 with broad spectrum antibiotic activity, its Latin systematic name：Micromonospora
Sp. depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, depositary institution address：Court of Beijing
The positive institute 3 of area's North Star West Road 1, Institute of Microorganism, Academia Sinica, No. CGMCC：12140, preservation date：2 months 2016
No. 18.
Described marine actinomycete S-19-3, micrococcus luteus (Micrococcus luteus), Escherichia coli are folded to gamboge eight
(Escherichia coli), pseudomonas aeruginosa (Pseudomonas aeruginosa), Candida albicans (Candida
Albicans) there is fungistatic effect, and contain PKS I genes.
The marine actinomycete S-19-3 SEQ ID No. with 16S rDNA:1 sequential structure；PKS I have SEQ ID
No.:2 amino acid sequence.
The marine actinomycete S-19-3 of present invention preparation method, the top layer for taking Jiaozhou Bay to observe erect-position collection sinks
Product thing, is pre-processed with dry heating method, is diluted to pure after various concentrations are cultivated 14-21 days on potassium bichromate screening and culturing medium
Change；Strain fermentation is carried out, its substrate mycelium is in brown color, and aerial hyphae is in Chinese red.
It is described as follows：
Take Jiaozhou Bay to observe the surface deposit of erect-position collection, pre-processed with dry heating method, is diluted to different dense
Degree is purified after being cultivated 14-21 days on potassium bichromate screening and culturing medium.Strain fermentation is carried out, is spread using Odontothrips loti and the scraps of paper
Method detects Activities of Fermentation Broth.Strain morphology feature is observed, physiological and biochemical test is carried out.Strain gene group is extracted, 16S is expanded
Two sections of sequences are compared by rDNA and PKS I genes respectively.
S-19-3 is good antibacterial to being respectively provided with for examination M.luteus, E.coli, P.aeruginosa and C.albicans
Effect.By morphologic observation, growing on optimal ISP4 culture mediums, S-19-3 substrate myceliums are in brown color, aerial hyphae is in
Chinese red.By physiological and biochemical test, it is found that S-19-3 can not liquefy gelatin, it is impossible to solidification and peptonized milk, shallow lake can be hydrolyzed
Powder, does not produce hydrogen sulfide；In carbon source experiment, glucose and arabinose can only be utilized.Pass through the identification to 16S rDNA, knot
Features described above is closed, it is micromonospora to identify actinomyces S-19-3.Amplification to its PKS I gene discloses its production polyketide
Potentiality.
It is an advantage of the invention that：
Marine actinomycete S-19-3 has broad spectrum antibiotic activity, to for examination gram-positive bacteria M.luteus, gram-negative
Property bacterium E.coli and P.aeruginosa, fungi C.albicans are respectively provided with good fungistatic effect.From marine actinomycete S-19-
The PKS I genes amplified in 3 disclose the potentiality that it produces polyketide.
Marine actinomycete S-19-3 with broad spectrum antibiotic activity, is isolated from the top layer of Jiaozhou Bay observation erect-position collection
Deposit；Preprocess method is dry heating method, and separation inhibitor is potassium bichromate；Its substrate mycelium is in brown color, and aerial hyphae is in
Chinese red.Identify that the bacterium is micromonospora with reference to bio-chemical characteristics and 16S rDNA.
Marine actinomycete S-19-3 has broad spectrum antibiotic activity, and micrococcus luteus, gram are folded to gram-positive bacteria gamboge eight
Negative bacterium E. coli and pseudomonas aeruginosa, fungi Candida albicans are respectively provided with good fungistatic effect.
Marine actinomycete S-19-3, amplifies I type polyketide synthase genes from its genome, discloses it and produces polyketide
The potentiality of thing.
Brief description of the drawings
Fig. 1：S-19-3 cultivates basal growth schematic diagram in ISP4
The form of bacterial strain S-19-3 28 DEG C of cultures two weeks on ISP4 culture mediums is as shown in Figure 1.Born when cultivating 6 days in base
Mycelia, in shallow crocus, hard difficulty is provoked；Deep Chinese red spore is born when cultivating 10 days, hereafter bacterial strain surface is that spore is surrounded,
In black.
Fig. 2：The phyletic evolution development tree of bacterial strain S-19-3 and related strain based on 16S rDNA
On phyletic evolution development tree, the new bacterium Micromonospora that bacterial strain S-19-3 is separated with Spain scholar
Recently, its sequence homology is up to 98% to lupini str.Lupac 08 (DSM 44870) affiliation.On evolution tree node
Numeral is 53, defines numeral 66 less than mutation or subspecies, it is probably the novel species in micromonospora to show the bacterium.
Embodiment
1. the collection and pretreatment of sample
Sample collection information：The surface deposit gathered in 9 different observation erect-positions of Jiaozhou Bay is big by Chinese Sea
Foreignize theory and provided with key lab of the engineering technology Ministry of Education in the sea of learning.By the Sample storage collected in 4 DEG C of Low-temperature Ices
In case, preserved for a long time with aseptic plastic bag.In use, 10mL bottom sediment samples are laid in sterile petri dish, 80 DEG C or
120 DEG C of dry heat treatment 1-2h, are finely ground to do separation after powder, dilution stand-by.
2. the separation of marine actinomycete
Flat board gradient dilution method is mainly taken in the separation of marine actinomycete, comprises the following steps that：
(1) the sample 10g of dry heat treatment is weighed, is added in 90mL antiseptic sea waters, it is quiet after 150r/min concussions 20min
Put 30min and take supernatant, 10 are diluted to step by step-2、10-3、10-4, respectively take 100 microlitres of selective flat boards of above-mentioned of coating, Mei Geti
Degree sets 3 repetitions；
(2), to suppress the growth of bacterium and fungi, it is put into 28 DEG C of culture containing 0.0075% potassium bichromate in culture medium
Culture 14d~21d is inverted in case；
(3) bacterium colony of picking different shape is to being cultivated on actinomyces screening and culturing medium flat board, then by the bacterium of separation
Fall the line purifying repeatedly on flat board, until obtaining the single culture of colonial morphology, be named as S-19-3.
3. Determination of Antibacterial Activity
S-19-3 Activities of Fermentation Broth is detected using Oxford cup and disk diffusion method, is comprised the following steps that：
(1) Odontothrips loti
1) 4~6 oxen are directly vertically put on solid medium (as LB, fungi is YPD to bacterium) surface using sterile working
Tianjin cup, gently pressurizes, it is contacted tight with culture medium,
2) S-19-3 zymotic fluids to be detected (centrifuging and taking supernatant) are added in cup, 200 μ L can be typically filled, not make outside it
Overflow.
3) rearmounted 37 DEG C (bacteriums) or 28 DEG C (fungi) are filled it up with and cultivate 2~3d, result is observed, measures inhibition zone with a yardstick straight
Footpath.
(2) disk diffusion method
1) thicker smooth filter paper is taken, a diameter of 6mm filter paper is made of card punch, aseptic filter paper piece is uniformly placed
In biological standardization flat board, per plate 4~8.
2) 10 μ L S-19-3 zymotic fluids (centrifuging and taking supernatant) are taken to be placed on the filter paper in biological standardization flat board, by life
Thing level(l)ing plate is first placed in 1~2h in 4 DEG C of refrigerators.Cultivate and observe measurement antibacterial circle diameter.
Bacteriostatic diameters of the S-19-3 of table 1 to strains tested
4. Morphological observation
After S-19-3 is cultivated 2~3 weeks on glucose-asparagine culture medium under the conditions of 28 DEG C, pass through light microscope
Observe mycelium morphology.With reference to international streptomycete planning (International Streptomyces Projects, ISP)
Standard method, accepted standard culture medium is carried out, in ISP2, ISP3, ISP4, ISP5, nutrient agar, Czapek's agar, potato
The typical unwrapping wire bacterium culture medium of seven kinds of agar is cultivated, colonial morphology, the change of mycelia in observation incubation.
The S-19-3 morphological features of table 2
5. bio-chemical characteristics
Content includes：Gelatin liquefaction, milk solidification are with peptonizing, Starch Hydrolysis, hydrogen sulfide are produced and utilization of carbon source is tested, its
Middle carbon source has glucose, fructose, xylose, rhamnose, mannitol, sucrose, arabinose, gossypose and inositol.
The S-19-3 physiological and biochemical properties of table 3
6. 16S rDNA sequencings and systematic evolution tree are built
Marine actinomycete S-19-3 genomic DNAs are extracted, 16S rDNA is expanded using bacterial universal primers and purifies sequencing,
Sequencing result carries out tetraploid rice by NCBI Blastn programs, using MEGA5.1 softwares, uses Neighbor-
Joining methods set up phylogenetic tree.By sequence analysis, the new bacterium that S-19-3 is separated with Spain scholar is found
Micromonospora lupini str.Lupac 08 (DSM 44870) homology is up to 98%.With reference to its morphological feature and
Physiological and biochemical property, preliminary judgement S-19-3 is micromonospora.
The amplification of 7.PKS I genes
PKS I genes are expanded using degenerate primer, after obtained fragment purification, transformation experiment is carried out and is sequenced,
Sequencing result carries out amino acid sequence homology comparison by NCBI Blastp programs, using MEGA5.1 softwares, uses
Neighbor-Joining methods set up phylogenetic tree.By sequence analysis, find S-19-3 PKS I sequences with
Micromonospora subsp.carbonacea Luedemann and Brodsky 1965 PKS I sequences (NCBI
Reference Sequence:) and polyketide synthase sequences (NCBI Reference WP_052503806.1
Sequence:WP_052503807.1) it is closer to.
SEQ ID No.:1
16S rDNA
CGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAGCGTT
GCTGATCTGCGATTACTAGCGACTCCGACTTCACGGGGTCGAGTTGCAGACCCCG
ATCCGAACTGAGACCGGCTTTTTGGGATTCGCTCCACCTCACGGTATCGCAGCCC
ATTGTACCGGCCATTGTAGCATGCGTGAAGCCCTGGACATAAGGGGCATGATGAC
TTGACGTCATCCCCACCTTCCTCCGAGTTGACCCCGGCAGTCTTCGATGAGTCCCC
GCCATAACGCGCTGGCAACATCGAACGAGGGTTGCGCTCGTTGCGGGACTTAACC
CAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTGACCGCCCCCG
AAGGACCCCCCATCTCTGAAGGTTTTGCGGCCATGTCAAACCCAGGTAAGGTTCT
TCGCGTTGCATCGAATTAATCCGCATGCTCCGCCGCTTGTGCGGGCCCCCGTCAAT
TCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGCGCTTAATGCGTTA
GCTGCGGCACAGAGAACCGGAGAGGCCCCCCACACCTAGCGCCCAACGTTTACA
GCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGC
GTCAGTATCGGCCCAGAGACCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTGC
GCATTTCACCGCTACACCAGGAATTCCAGTCTCCCCTACCGAACTCTAGCCTGCCC
GTATCGACCGCAGGCTTGGGGTTGAGCCCCAAGTTTTCACGGTCGACGCGACAAG
CCGCCTACGAGCTCTTTACGCCCAATAAATCCGGACAACGCTCGCGCCCTACGTC
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGCGCTTCTTCTGCAGGTACCGTCACT
TGCGCTTCGTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACG
CGGCGTCGCTGCATCAGGCTTCCGCCCATTGTGCAATATTCCCCACTGCTGCCTCC
CGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCC
GGCTACCCGTCGTCGCCTTGGTAGGCCATCACCCCACCAACAAGCTGATAGGCCG
CGAGCCCATCCCAGGCCGAAAAACTTTCCACCCCCAGTCATGCGACCAAAGGTTG
TATCCGGTATTAGCCCCCGTTTCCGAGGGTTATCCCAAAGCCTAGGGCAGGTTGC TCACGTGTTACTCAC
SEQ ID No.:2
PKS I amino acid
Met T I D T L C S S S L V A L H T A V R S I R S V E C D Q A I V A G V
H L AMet S P Q Y F Q L G S R L R S F S P T G A S R A F D A A A D G F V P G E
G V V G V V V K P L A H A V H D E D Q I H G V I P V T A V N H G G R T S G L T
V P S S S A Q Q D V I L A A L R D A G V G P E G I G Met V D T H G T S T N G T
S L N H P N A T H N A H N H R A A P P R G I K I S P I Stop
Claims (3)
1. one plant of marine actinomycete S-19-3 with broad spectrum antibiotic activity, it is characterized in that Latin systematic name：
Micromonospora lupini, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, protects
Hide unit address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, No. CGMCC：12140,
Preservation date：2 months 2016 No. 18.
2. marine actinomycete S-19-3 as claimed in claim 1, it is characterized in that the bacterium to M.luteus, E.coli,
P.aeruginosa or C.albicans have fungistatic effect, and contain PKS I genes.
3. the preparation method of the marine actinomycete S-19-3 described in claim 1 or 2, it is characterized in that taking Jiaozhou Bay observation station
The surface deposit of position collection, is pre-processed with dry heating method, is diluted to various concentrations and is trained on potassium bichromate screening and culturing medium
Purified after supporting 14-21 days；Strain fermentation is carried out, its substrate mycelium is in brown color, and aerial hyphae is in Chinese red.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220198A (en) * | 2018-01-29 | 2018-06-29 | 浙江海洋大学 | It is a kind of that there is the marine actinomycete for inhibiting fungi activity |
| CN109280692A (en) * | 2018-10-31 | 2019-01-29 | 广东药科大学 | A kind of the antifungal activity screening technique and its fermentation process of blattaria endogeny rayungus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302482A (en) * | 2007-11-20 | 2008-11-12 | 大连理工大学 | Marine streptomyces S187 having wide-spectrum antibacterial activity |
| CN101363022A (en) * | 2008-04-18 | 2009-02-11 | 中国科学院上海有机化学研究所 | Biological synthesis gene cluster of tetrokacin A and use thereof |
| CN103114064A (en) * | 2013-03-08 | 2013-05-22 | 浙江省柑桔研究所 | Marine actinomycete with antibacterial activity to multiple plant pathogens |
-
2016
- 2016-09-29 CN CN201610865217.8A patent/CN107299064B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302482A (en) * | 2007-11-20 | 2008-11-12 | 大连理工大学 | Marine streptomyces S187 having wide-spectrum antibacterial activity |
| CN101363022A (en) * | 2008-04-18 | 2009-02-11 | 中国科学院上海有机化学研究所 | Biological synthesis gene cluster of tetrokacin A and use thereof |
| CN103114064A (en) * | 2013-03-08 | 2013-05-22 | 浙江省柑桔研究所 | Marine actinomycete with antibacterial activity to multiple plant pathogens |
Non-Patent Citations (2)
| Title |
|---|
| 张孟等: "海洋抑菌放线菌Y15的筛选、鉴定及其代谢产物理化性质", 《微生物学通报》 * |
| 齐麟: "两株海洋放线菌的筛选鉴定及其次级代谢产物研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220198A (en) * | 2018-01-29 | 2018-06-29 | 浙江海洋大学 | It is a kind of that there is the marine actinomycete for inhibiting fungi activity |
| CN109280692A (en) * | 2018-10-31 | 2019-01-29 | 广东药科大学 | A kind of the antifungal activity screening technique and its fermentation process of blattaria endogeny rayungus |
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