CN107299007A - A kind of biological fruit-vegetable cleaning agent and preparation method - Google Patents
A kind of biological fruit-vegetable cleaning agent and preparation method Download PDFInfo
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- CN107299007A CN107299007A CN201610234760.8A CN201610234760A CN107299007A CN 107299007 A CN107299007 A CN 107299007A CN 201610234760 A CN201610234760 A CN 201610234760A CN 107299007 A CN107299007 A CN 107299007A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/40—Products in which the composition is not well defined
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/02—Inorganic compounds
- C11D7/04—Water-soluble compounds
- C11D7/10—Salts
- C11D7/12—Carbonates bicarbonates
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/265—Carboxylic acids or salts thereof
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/268—Carbohydrates or derivatives thereof
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Abstract
The present invention relates to a kind of biological fruit-vegetable cleaning agent and preparation method.The biological fruit-vegetable cleaning agent agent is comprised the following raw materials by weight percent：Protease 1% ~ 5%, phytase 1% ~ 5%, lipase 1% ~ 5%, laccase 1% ~ 5%, catalase 1% ~ 5%, enzymatic protective reagent 5% ~ 10%, chitosan 0.1% ~ 1%, citric acid 0.1% ~ 1%, calcium propionate 1% ~ 5%, sodium acid carbonate 5% ~ 10% and deionized water 50% ~ 80%.Preparation method is：Citric acid, sodium acid carbonate, calcium propionate are dissolved in deionized water successively; stir; formed after weakly alkaline solution and add chitosan continuation stirring; after add enzymatic protective reagent; make the solution one buffer system of formation, sequentially add protease, phytase, lipase, laccase, catalase, be slowly stirred uniform; plate-frame filtering, its clear liquid is finished product.Above-mentioned biological fruit-vegetable cleaning agent carries out processing fruits and vegetables using the cooperative effect between enzyme preparation and enzymatic protective reagent, can make the degradeds such as residues of pesticides, wax, germ in fruits and vegetables or kill, so as to ensure the edible safety of fruit and vegetable food.
Description
Technical field
The present invention relates to a kind of biological fruit-vegetable cleaning agent, and in particular to a kind of green safety cleaning agent of biological removal residues of pesticides, belongs to daily chemical product technical field.
Background technology
Today's society, excessive pursuit of the people to economic benefit causes the lack of standardization using increasingly severe of agricultural chemicals, although a large amount of uses of agricultural chemicals improve the yield of gourd, fruit and vegetable and reduce the generation of pest and disease damage, but residues of pesticides have seriously endangered the health of the mankind, vegetables and anti-season fruit most growth periods are short, pest and disease damage is than more serious, spraying pesticide diseases prevention is important step in fruits and vegetables production, agricultural chemicals is used for multiple times in planting process, it is short plus plucking time after dispenser, cause vegetable melon and fruit residues of pesticides severe overweight.
As the improvement of people's living standards, people are food-safe and environmental protection consciousness is gradually strengthened, the Pesticide Residue of surface of agricultural products is increasingly subject to the common concern of the public.Therefore, the residues of pesticides of surface of agricultural products, microorganism and dirt can effectively be removed by developing, and not cause secondary pollution, and free from environmental pollution, and fast and easily strong detergent receives much concern for cleaning.
The content of the invention
Based on this, it is necessary to provide biological fruit-vegetable cleaning agent and preparation method that a kind of high-performance bio removes residues of pesticides and microorganism.
A kind of biological fruit-vegetable cleaning agent and preparation method, it is characterised in that comprise the following raw materials by weight percent：
Protease
1%~5%；
Phytase
1%~5%；
Lipase 1% ~ 5%；
Laccase enzyme 1% ~ 5%；
Catalase
1%~5%；
Enzymatic protective reagent
5%~10%；
Chitosan
0.1%~1%；
Citric acid
0.1%~1%；
Calcium propionate
1%~5%；
Sodium acid carbonate
5% ~ 10% and
Deionized water
50%~80%；
In one of the embodiments, a kind of biological fruit-vegetable cleaning agent is comprised the following raw materials by weight percent：
Protease
2%~3%；
Phytase
3%~4%；
Lipase
2%~4%；
Laccase enzyme 1% ~ 3%；
Catalase
3%~5%；
Enzymatic protective reagent
6%~8%；
Chitosan
0.3%~0.5%；
Citric acid
0.2%~0.8%；
Calcium propionate 3% ~ 5%；
Sodium acid carbonate
5% ~ 8% and
Deionized water
55%~80%。
In one of the embodiments, a kind of biological fruit-vegetable cleaning agent is comprised the following raw materials by weight percent：
Protease
5%；
Phytase
1%；
Lipase
3%；
Laccase enzyme 2%；
Catalase
4%；
Enzymatic protective reagent 10%；
Chitosan
0.5%；
Citric acid
0.5%；
Calcium propionate
1%；
Sodium acid carbonate
8% and
Deionized water
65%。
A kind of biological fruit-vegetable cleaning agent preparation method, it is characterised in that comprise the following steps：
The biological fruit-vegetable cleaning agent comprised the following raw materials by weight percent：
Protease
1%~5%；
Phytase 1% ~ 5%；
Lipase
1%~5%；
Laccase enzyme 1% ~ 5%；
Catalase
1%~5%；
Enzymatic protective reagent
5%~10%；
Chitosan
0.1%~1%；
Citric acid
0.1%~1%；
Calcium propionate
1%~5%；
Sodium acid carbonate
5% ~ 10% and
Deionized water 50% ~ 80%；
Citric acid, sodium acid carbonate, calcium propionate are dissolved in deionized water successively; stir; speed of agitator is 30 ~ 50rmp; form weakly alkaline solution; its pH value is maintained at 7.2 ~ 7.8; chitosan is added afterwards to stir, enzymatic protective reagent is added afterwards, makes the solution one buffer system of formation; sequentially add protease, phytase, lipase, laccase, catalase; it is slowly stirred uniformly, mixing time is 20 ~ 40min, rear plate-frame filtering; a diameter of 0.1 ~ the 0.2mm of sheet frame filter membrane; solution after filtering is detected, filling, as finished product.
The beneficial effects of the invention are as follows：
1st, the supplementary material that the present invention is used is the product of green safety, meets food additives GB2760-2011 national standards, used main component is biological agent, pollution-free, nontoxic, and secondary pollution is not resulted in.
2nd, significantly, the clearance of the residues of pesticides shown fruits and vegetables, especially organophosphorus insecticide and fruit wax is up to more than 96% for product cleaning performance of the invention.Chitosan, calcium propionate in the present invention are food additives, can effectively kill pollution of the pathogen to food.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, the embodiment below to the present invention is described in detail.Many details are elaborated in the following description to fully understand the present invention.But the invention can be embodied in many other ways as described herein, those skilled in the art can make similar improvements without departing from the spirit of the invention, therefore the present invention is not limited to the specific embodiments disclosed below.
The biological fruit-vegetable cleaning agent of the embodiment provided according to the chemical composition to melon and fruit agricultural chemicals and residue analysis, the present invention, is comprised the following raw materials by weight percent：
Protease
1%~5%；
Phytase
1%~5%；
Lipase
1%~5%；
Laccase enzyme 1% ~ 5%；
Catalase
1%~5%；
Enzymatic protective reagent
5%~10%；
Chitosan 0.1% ~ 1%；
Citric acid
0.1%~1%；
Calcium propionate
1%~5%；
Sodium acid carbonate
5% ~ 10% and
Deionized water
50%~80%。
By above-mentioned raw materials mixture, by multi-factor and multi-level orthogonal test, the proportioning being more highly preferred to for determining various enzymes is：
Protease
2%~3%；
Phytase
3%~4%；
Lipase
2%~4%；
Laccase enzyme 1% ~ 3%；
Catalase
3%~5%；
Enzymatic protective reagent
6%~8%；
Chitosan
0.3%~0.5%；
Citric acid
0.2%~0.8%；
Calcium propionate
3%~5%；
Sodium acid carbonate
5% ~ 8% and
Deionized water
55%~80%。
Citric acid, sodium acid carbonate, calcium propionate are dissolved in deionized water successively; stir; speed of agitator is 30 ~ 50rmp; form weakly alkaline solution; its pH value is maintained at 7.5 ~ 7.8; chitosan is added afterwards to stir, enzymatic protective reagent is added afterwards, makes the solution one buffer system of formation; sequentially add protease, phytase, lipase, laccase, catalase; it is slowly stirred uniformly, mixing time is 20 ~ 40min, rear plate-frame filtering; a diameter of 0.1 ~ the 0.2mm of sheet frame filter membrane; solution after filtering is detected, filling, as finished product.
Wherein, protease preferably is neutral proteinase；
It is preferred that enzymatic protective reagent be trehalose；
It is preferred that chitosan carboxymethyl chitosan；
It is preferred that weakly acidic solution pH value be 7.2 ~ 7.8；
The rotating speed being preferably slowly stirred is 30 ~ 50rmp；
The time being preferably slowly stirred is 20 ~ 40min；
It is preferred that plate-frame filtering, a diameter of 0.1 ~ 0.2mm of its filter membrane；
Citric acid, sodium acid carbonate, calcium propionate will be added in product, the pH value of product is tuned into alkalescent, citric acid and sodium acid carbonate can form buffering pair, make the product more stable.
Sodium acid carbonate is electrolyzed to produce bicarbonate ion and sodium ion in water, further electrolysis is hydrogen ion and carbanion to bicarbonate ion, hydrogen positively charged, which is added to the water, can combine electronegative hydrone O 2, become OH- for week-base water, general most agricultural chemicals is all acid, therefore in week-base water and outside acidity, moreover it is possible to accelerate to remove the agricultural chemicals being attached on vegetables and fruits；In addition, Na is positively charged, it is put into inside running water, sodium chloride, that is, salt can be become with electronegative chloropexia in water, which solve the problem of running water chlorine poison.
Calcium propionate is the preservative currently used for food, can effectively, safety, quickly kill mould, saccharomycete and bacterium, calcium propionate can be absorbed by the body by being metabolized in vivo with other fat as, and supplemented with necessary calcium ion.
Chitosan is the product that chitin takes off N- acetyl group, the shell of the shell-fish from ocean, and a significant properties of chitosan is adsorption capacity, can adsorb the heavy metal in fruits and vegetables, and it is efficient chelate medium to have been demonstrated that chitosan.Due to the interaction of negative electrical charge on the positive charge and bacterial cell membrane of chitosan molecule, make intracellular protease and other compositions leakage, so as to reach antibacterial, bactericidal action.Simultaneously combined because chitosan can penetrate into the core of bacterium with DNA, suppress mRNA synthesis, so as to hinder the synthesis of mRNA and protein, reach antibacterial action.Chitosan also has a very important effect to be exactly preserving fruit and vegetable utilizing, and the purpose of preserving fruit and vegetable utilizing is mainly to maintain fruits and vegetables after harvesting until shelf life, can maintain normal quality, taste, nutritional ingredient and outward appearance, improve its commodity value.Coating-film fresh-keeping is carried out with chitosan, its film layer has permeability, water preventing ability, penetration resistance can be increased to various gas molecules, form a kind of micro- controlled atmosphere environment, it is the carbon dioxide content increase in fruits and vegetables tissue, oxygen content reduction, it is suppressed that the respiratory metabolism of fruits and vegetables and moisture loss, slow down fruits and vegetables tissue and structure aging, so that effectively extension fruits and vegetables adopt the rear life-span.Chitosan hardly possible is digested and assimilated by human intestines and stomach, after people takes in them in vivo, they can be with generating complex compound equivalent to lipoid substances such as the triglycerides of sole mass manyfold, aliphatic acid, bile acid and cholesterol, the complex compound is not hydrolyzed by hydrochloric acid in gastric juice, system absorption is not digested, so as to hinder this kind of material of absorption of human body, it is allowed to wear intestines and cross and excrete.
Lipase can remove the wax of fruit and vegetable surfaces, natural plants（Including fruit and vegetable）All there is a kind of wax in surface, this wax is a kind of instinct that plant resists external world active defense, and commercially available fruit is in order to fresh-keeping, the work that will hit the person ceride, these natural and artificial waxs are without effective degraded, decomposable asymmetric choice net agriculture the effective elements of the medicine is difficult to be combined with agricultural chemicals, so abolishing the wax of fruit and vegetable surfaces with lipase, lipase is a kind of special ester linkage hydrolyzing enzyme, it may act on the ester bond of triglycerides, triglycerides is degraded to diglyceride, monoglyceride, glycerine and aliphatic acid.
Phytase is catalysis phytic acid and its saline hydrolysis are inositol and phosphoric acid（Salt）Class of enzymes general name, belong to orthophosphoric ester monohydrolase.Most of organophosphor can be changed into Phos by phytase, so as to play a part of organophosphor in degrading pesticide, poisonous organophosphor can be changed into Phos.
Laccase is a kind of polyphenol oxidase, and the suitable substrate molecule of laccase is phenols, and armaticity and fatty amine.And most agricultural chemicals are amine or aromatic compounds, the process of Laccase Catalyzed is the one-electron oxidation of substrate, generates corresponding living radical.Laccase can be catalyzed phenols, arylamine class, carboxylic acids, steroid hormone, biochrome, metallo-organic compound and non-aldehydes matter generation quinones, carbonyls and water, so as to reach the purpose of degrading pesticide.
We have surprisingly found that neutral proteinase and catalase use in conjunction are very good to the action effect of phoxim, and the organophosphor of high poison is changed into low toxicity or innocuous substance under study for action.
Trehalose is the natural protective agent of enzyme, because the essence of enzyme is protein, so protease can slowly decompose other enzyme preparations in aqueous, we have discovered that, when protease and other enzymes are dissolved into the aqueous solution, add a certain amount of trehalose and add citric acid, sodium acid carbonate, calcium propionate, in the cushioning liquid formed after chitosan, it can play a part of being passivated protease, its protease is set not occur degradation reaction with other enzymes in such buffer system, in application process, the such buffer system of destruction, such as when cleaning fruits and vegetables, it is after fruits and vegetables cleaning agent dilutes to add substantial amounts of water, just such buffer system is destroyed, make the activity of protease and can discharge well, as long as there is the degradable substrate of protease in application process, protease would not degrade other enzyme preparations.
It follows that trehalose, citric acid, sodium acid carbonate, calcium propionate, chitosan complement each other in this formula, worked in coordination with enzyme preparation and play respective effect, can combined again and play a part of superposition.
Further illustrated below by way of specific embodiment.
Embodiment 1
The percentage by weight of fruits and vegetables cleaning agent each component is respectively：Comprise the following raw materials by weight percent：
Protease 5%, phytase 1%, lipase 3%, laccase enzyme 2%, catalase 4%, enzymatic protective reagent 10%, chitosan 0.5%, citric acid 0.5%, calcium propionate 1%, sodium acid carbonate 8% and deionized water 65%.
By citric acid 0.5kg, sodium acid carbonate 8kg, calcium propionate 1kg is dissolved in 65kg deionized waters successively, stir, speed of agitator is 30rmp, form weakly alkaline solution, its pH value is maintained at 7.2, chitosan 0.5kg is added afterwards to stir, enzymatic protective reagent 10kg is added afterwards, make the solution one buffer system of formation, sequentially add protease 5kg, phytase 1kg, lipase 3kg, laccase 2kg, catalase 4kg, it is slowly stirred uniform, mixing time is 20min, plate-frame filtering, the a diameter of 0.2mm of sheet frame filter membrane, solution detection after filtering, it is filling, as finished product 100kg.
Embodiment 2
The percentage by weight of fruits and vegetables cleaning agent each component is respectively：Comprise the following raw materials by weight percent：
Protease 1%, phytase 5%, lipase 5%, laccase enzyme 1%, catalase 1%, enzymatic protective reagent 5%, chitosan 0.1%, citric acid 0.1%, calcium propionate 5%, sodium acid carbonate 5% and deionized water 71%.
By citric acid 0.1kg, sodium acid carbonate 5kg, calcium propionate 5kg is dissolved in 71kg deionized waters successively, stir, speed of agitator is 40rmp, form weakly alkaline solution, its pH value is maintained at 7.5, chitosan 0.1kg is added afterwards to stir, enzymatic protective reagent 5kg is added afterwards, make the solution one buffer system of formation, sequentially add protease 1kg, phytase 5kg, lipase 5kg, laccase 1kg, catalase 1kg, it is slowly stirred uniform, mixing time is 30min, plate-frame filtering, the a diameter of 0.3mm of sheet frame filter membrane, solution detection after filtering, it is filling, as finished product 100kg.
Embodiment 3
The percentage by weight of fruits and vegetables cleaning agent each component is respectively：Comprise the following raw materials by weight percent：
Protease 3 %, phytase 3%, lipase 1%, laccase enzyme 5%, catalase 5%, enzymatic protective reagent 8%, chitosan 1%, citric acid 1%, calcium propionate 3%, sodium acid carbonate 8% and deionized water 62%.
By citric acid 1kg, sodium acid carbonate 8kg, calcium propionate 3kg is dissolved in 62kg deionized waters successively, stir, speed of agitator is 50rmp, form weakly alkaline solution, its pH value is maintained at 7.8, chitosan 1kg is added afterwards to stir, enzymatic protective reagent 8kg is added afterwards, make the solution one buffer system of formation, sequentially add protease 3 kg, phytase 3kg, lipase 1kg, laccase 5kg, catalase 5kg, it is slowly stirred uniform, mixing time is 40min, plate-frame filtering afterwards, the a diameter of 0.3mm of sheet frame filter membrane, solution detection after filtering, it is filling, as finished product 100kg.
Embodiment 4
The percentage by weight of fruits and vegetables cleaning agent each component is respectively：Comprise the following raw materials by weight percent：
Protease 3 %, phytase 4%, lipase 4%, laccase enzyme 3%, catalase 5%, enzymatic protective reagent 8%, chitosan 0.5%, citric acid 0.8%, calcium propionate 5%, sodium acid carbonate 8% and deionized water 58.7%.
By citric acid 0.8kg, sodium acid carbonate 8kg, calcium propionate 5kg is dissolved in 58.7kg deionized waters successively, stir, speed of agitator is 30rmp, form weakly alkaline solution, its pH value is maintained at 7.6, chitosan 0.5kg is added afterwards to stir, enzymatic protective reagent 8kg is added afterwards, make the solution one buffer system of formation, sequentially add protease 3 kg, phytase 4kg, lipase 4kg, laccase 3kg, catalase 5kg, it is slowly stirred uniform, mixing time is 30min, plate-frame filtering, the a diameter of 0.2mm of sheet frame filter membrane, solution detection after filtering, it is filling, as finished product 100kg.
The test method and application effect of the present invention is as follows：
1st, test method：
Fresh cowpea is soaked in parathion-methyl, cypermethrin and the arprocarb blended aqueous solution（Each pesticide concentration is 200ppm）, take out after 20min, hang on room temperature shady place and dry；The vegetable sample prepared is divided into 3 groups, do not wash, wash, 0.1% fruits and vegetables cleaning fluid（Embodiment 1 ~ 4）Washing is respectively 1 group, every group 2 points, every part of 100g；It is parallel that 3 repetitions have been done in above pre-treatment.Follow-up test scheme according to《The measure remained in vegetables and fruit organophosphor, organochlorine, pyrethroid and carbamate chemicals for agriculture more》NY/T 761-2008 standards are carried out.
2nd, result of the test such as table 1：
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but therefore can not be interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of biological fruit-vegetable cleaning agent, it is characterised in that comprise the following raw materials by weight percent：
Protease
1%~5%；
Phytase
1%~5%；
Lipase
1%~5%；
Laccase enzyme
1%~5%；
Catalase
1%~5%；
Enzymatic protective reagent
5%~10%；
Chitosan
0.1%~1%；
Citric acid
0.1%~1%；
Calcium propionate
1%~5%；
Sodium acid carbonate
5% ~ 10% and
Deionized water
50%~80%。
2. a kind of biological fruit-vegetable cleaning agent according to claim 1, it is characterised in that comprise the following raw materials by weight percent：
Protease
2%~3%；
Phytase
3%~4%；
Lipase
2%~4%；
Laccase enzyme
1%~3%；
Catalase
3%~5%；
Enzymatic protective reagent
6%~8%；
Chitosan
0.3%~0.5%；
Citric acid
0.2%~0.8%；
Calcium propionate
3%~5%；
Sodium acid carbonate
5% ~ 8% and
Deionized water
55%~80%。
3. a kind of biological fruit-vegetable cleaning agent according to claim 1, it is characterised in that comprise the following raw materials by weight percent：
Protease
5%；
Phytase
1%；
Lipase
3%；
Laccase enzyme
2%；
Catalase
4%；
Enzymatic protective reagent
10%；
Chitosan
0.5%；
Citric acid
0.5%；
Calcium propionate
1%；
Sodium acid carbonate
8% and
Deionized water
65%。
4. a kind of preparation method of biological fruit-vegetable cleaning agent, it is characterised in that comprise the following steps：
（1）A kind of biological fruit-vegetable cleaning agent comprised the following raw materials by weight percent：
Protease
1%~5%；
Phytase
1%~5%；
Lipase
1%~5%；
Laccase enzyme
1%~5%；
Catalase
1%~5%；
Enzymatic protective reagent
5%~10%；
Chitosan
0.1%~1%；
Citric acid
0.1%~1%；
Calcium propionate
1%~5%；
Sodium acid carbonate
5% ~ 10% and
Deionized water
50%~80%；
（2）Citric acid, sodium acid carbonate, calcium propionate are dissolved in deionized water successively, stirred, weakly alkaline solution is formed; add chitosan to stir, enzymatic protective reagent is added afterwards, make the solution one buffer system of formation; sequentially add protease, phytase, lipase; laccase, catalase is slowly stirred uniform; plate-frame filtering; its clear liquid is detected, filling, as finished product.
5. a kind of biological fruit-vegetable cleaning agent according to claim 1, it is characterised in that the protease is neutral proteinase.
6. a kind of biological fruit-vegetable cleaning agent according to claim 1, it is characterised in that the enzymatic protective reagent is trehalose.
7. a kind of biological fruit-vegetable cleaning agent according to claim 1, it is characterised in that the chitosan is carboxymethyl chitosan.
8. the preparation method of a kind of biological fruit-vegetable cleaning agent according to claim 4, it is characterised in that the pH value of described weakly alkaline solution is 7.2 ~ 7.8.
9. the preparation method of a kind of biological fruit-vegetable cleaning agent according to claim 4, it is characterised in that the described rotating speed being slowly stirred is 30 ~ 50rmp.
10. the preparation method of a kind of biological fruit-vegetable cleaning agent according to claim 4, it is characterised in that described is slowly stirred uniform mixing time for 20 ~ 40min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300584A (en) * | 2018-01-25 | 2018-07-20 | 东营市浩瀚生化科技有限公司 | It is a kind of to remove vegetables and fruits and the residual cleaning agent and preparation method thereof with heavy metal ion of aquatic products agriculture |
| CN109266447A (en) * | 2018-10-30 | 2019-01-25 | 四川夏商生物科技有限公司 | A kind of biological cleanser and preparation method thereof |
| CN113881516A (en) * | 2020-07-02 | 2022-01-04 | 北京世城双清科技有限公司 | Complex enzyme cleaning agent |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281890A (en) * | 2000-06-01 | 2001-01-31 | 胡银安 | Method for removing residual pesticide from picked fruit and vegetable and its useful cleaning agent |
| CN1414083A (en) * | 2002-09-20 | 2003-04-30 | 汪正红 | Delexifying and cleaning agent for residual pesticide on fruits and vegetables |
| CN101077998A (en) * | 2006-05-25 | 2007-11-28 | 常锋 | Method for preparing cleaning agent for degrading fruit and vegetable residual pesticide |
| CN103168977A (en) * | 2013-02-05 | 2013-06-26 | 叶锦标 | Fruit and vegetable pesticide residue removing agent and preparation method thereof |
| CN104371853A (en) * | 2014-11-24 | 2015-02-25 | 馥稷生物科技发展（上海）有限公司 | Cleaning agent for natural fruit and vegetable on removing pesticide residue and killing bacteria |
| CN104450307A (en) * | 2014-12-09 | 2015-03-25 | 王先军 | Multipurpose surfactant-free cleaning agent for fruits, vegetables and dishes and method for producing multipurpose surfactant-free cleaning agent for fruits, vegetables and dishes |
| CN105385512A (en) * | 2015-12-15 | 2016-03-09 | 山东消博士消毒科技股份有限公司 | Low-foam multienzyme cleaning fluid for medical apparatus cleaning and preparation technology thereof |
-
2016
- 2016-04-15 CN CN201610234760.8A patent/CN107299007B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281890A (en) * | 2000-06-01 | 2001-01-31 | 胡银安 | Method for removing residual pesticide from picked fruit and vegetable and its useful cleaning agent |
| CN1414083A (en) * | 2002-09-20 | 2003-04-30 | 汪正红 | Delexifying and cleaning agent for residual pesticide on fruits and vegetables |
| CN101077998A (en) * | 2006-05-25 | 2007-11-28 | 常锋 | Method for preparing cleaning agent for degrading fruit and vegetable residual pesticide |
| CN103168977A (en) * | 2013-02-05 | 2013-06-26 | 叶锦标 | Fruit and vegetable pesticide residue removing agent and preparation method thereof |
| CN104371853A (en) * | 2014-11-24 | 2015-02-25 | 馥稷生物科技发展（上海）有限公司 | Cleaning agent for natural fruit and vegetable on removing pesticide residue and killing bacteria |
| CN104450307A (en) * | 2014-12-09 | 2015-03-25 | 王先军 | Multipurpose surfactant-free cleaning agent for fruits, vegetables and dishes and method for producing multipurpose surfactant-free cleaning agent for fruits, vegetables and dishes |
| CN105385512A (en) * | 2015-12-15 | 2016-03-09 | 山东消博士消毒科技股份有限公司 | Low-foam multienzyme cleaning fluid for medical apparatus cleaning and preparation technology thereof |
Non-Patent Citations (1)
| Title |
|---|
| 赵秀阁: "几种典型农药化合物及其残留的生物和化学降解的研究", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300584A (en) * | 2018-01-25 | 2018-07-20 | 东营市浩瀚生化科技有限公司 | It is a kind of to remove vegetables and fruits and the residual cleaning agent and preparation method thereof with heavy metal ion of aquatic products agriculture |
| CN109266447A (en) * | 2018-10-30 | 2019-01-25 | 四川夏商生物科技有限公司 | A kind of biological cleanser and preparation method thereof |
| CN113881516A (en) * | 2020-07-02 | 2022-01-04 | 北京世城双清科技有限公司 | Complex enzyme cleaning agent |
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| CN107299007B (en) | 2019-12-20 |
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