CN107287126A - A kind of isolation and purification method of yellow meat rod bacterium strain - Google Patents
A kind of isolation and purification method of yellow meat rod bacterium strain Download PDFInfo
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- CN107287126A CN107287126A CN201710550010.6A CN201710550010A CN107287126A CN 107287126 A CN107287126 A CN 107287126A CN 201710550010 A CN201710550010 A CN 201710550010A CN 107287126 A CN107287126 A CN 107287126A
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- rod bacterium
- meat rod
- yellow meat
- culture medium
- bacterium strain
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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Abstract
The present invention relates to a kind of isolation and purification method of yellow meat rod bacterium strain, including：(1) gather；(2) strain separating；(3) purify；(4) ordinary culture medium is adapted to.The present invention is simple to operate, further can develop and develop potential meat rod bacterium new resource food, has started the development of the potential new resource food meat rod bacterium of China and has developed the beginning.
Description
Technical field
The invention belongs to edible and medical fungi field, more particularly to a kind of isolation and purification method of yellow meat rod bacterium strain.
Background technology
China is one of bio-diversity most abundant country, and China is estimated to be 100,000 kinds of edible and medical fungis, has been realized
Only 1000 kinds or so, and only 100 kinds or so be utilized, it can be seen that most of wild edible and medical fungis are undiscovered, recognize
Know and utilize.Therefore it is one of important process of China edible and medical fungi worker to find more preferable edible and medical fungi.
Meat rod bacterium (Podostroma sp.) is a kind of with 16 sons special macro fungi species ascosporous, current generation
Meat rod Pseudomonas on the books only has 20 records on boundary, it can be seen that meat rod bacterium treasures degree.Meat rod bacterium China also not by
Vast majority of people's awareness and understanding, or even concept of most people for being engaged in fungus research also without this species, and China at present
Only several kinds are described and record, and do not have also isolate and purify the report that strain and cultivation etc. are utilized so far.According to Primary Study, meat
Rod bacterium has special edible and medical value, therefore how research isolates and purifies meat rod bacterium strain, corresponding so as to carry out
Developmental research, be exploitation edible and medical fungi new varieties, the important channel of developing new drug thing.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of isolation and purification method of yellow meat rod bacterium strain, this method
It is simple to operate, potential meat rod bacterium new resource food further can be developed and developed, the potential new resource food meat rod of China has been started
The development of bacterium and the exploitation beginning.
A kind of isolation and purification method of yellow meat rod bacterium strain of the present invention, including：
(1) collection is taken from the yellow meat rod bacterium in field liver moss clump, and surface residues is handled, pending strain
Separation；
(2) 16 sporangiums are taken in the middle part of yellow meat rod bacterium, be inoculated in the special culture medium prepared and sterilized in advance,
Culture 10-12 days is carried out under 20-23 DEG C of half-light, yellow meat rod bacterium strain is obtained；Wherein, the composition of special culture medium is：Potato
200g, glucose 10g, lactose 10g, peptone 10g, yeast extract 10g, potassium chloride 0.05g, sodium sulphate 0.15g, dipotassium hydrogen phosphate
0.5g, calcium chloride 0.025g, ammonium di-hydrogen phosphate 0.25g, 2% liquor ferri trichloridi 1.2ml, agar 25g, vitamin B1100mg、
Brewer's wort 15g, distilled water 1000ml；
(3) yellow meat rod bacterium strain transfer is cultivated into the culture medium with antibiotic under 23-25 DEG C of half-light
10-12 days, obtain the yellow meat rod bacterium strain by antibiotic-screening；Wherein, the composition of the culture medium with antibiotic is：Soil
Beans 200g, peptone 10g, yeast extract 10g, glucose 20g, agar 20g, sterilizing be separately added into 1% streptomysin, 1% penicillin,
1% each 1ml of gentamicin antibiotic；
(4) the yellow meat rod bacterium strain transfer for obtaining step (3) is carried out into PDA culture medium under 20-23 DEG C of half-light
Culture 10-12 days, you can.
The processing that carried out to surface residues in the step (1) includes clearing up, sterilizes, dries.
The preparation steps of special culture medium in the step (2) are：Potato filtering to boil water, is added after agar melts once
Peptone, the yeast extract melted is added, other raw materials are sequentially added, 1000ml, packing examination are settled to until completely dissolved
Sterilized after pipe standby.
Beneficial effect
The present invention is simple to operate, further can develop and develop potential meat rod bacterium new resource food, start China potential
The development of new resource food meat rod bacterium and the exploitation beginning.
Brief description of the drawings
Fig. 1 is wild yellow meat rod bacterium photo；
Fig. 2 is yellow meat rod bacterium strain photo.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
1) the wild yellow meat rod bacterium being grown in liver moss clump is collected：Yellow meat rod bacterium of the collection from field is taken, and to table
Face residue such as is cleared up, sterilized, being dried at the processing, pending strain separating.
2) special culture medium is prepared：Specific culture based component is potato 200g, glucose 10g, lactose 10g, peptone
10g, yeast extract 10g, potassium chloride 0.05g, sodium sulphate 0.15g, dipotassium hydrogen phosphate 0.5g, calcium chloride 0.025g, ammonium di-hydrogen phosphate
0.25g, 2% liquor ferri trichloridi 1.2ml, agar 25g, vitamin B1100mg, brewer's wort 15g, distilled water 1000ml.Prepare
Step is that potato filtering to boil water adds after agar melts and sequentially adds the peptone melted, yeast extract, sequentially adds it
Its raw material, waits and 1000ml is settled to after being completely dissolved, and is sterilized after packing test tube standby.
3) strain separating：16 sporangiums are taken in the middle part of from yellow meat rod bacterium, is inoculated in tweezers gripping and is prepared and sterilize in advance
Special culture medium in, culture 10 days or so is carried out under 23-25 DEG C of half-light, it is preliminary to obtain yellow meat rod bacterium strain.
4) purify：The yellow meat rod bacterium strain transfer tentatively obtained, to the culture medium with antibiotic, (composition is：Potato
200g, peptone 10g, yeast extract 10g, glucose 20g, agar 20g, sterilizing be separately added into 1% streptomysin, 1% penicillin,
1% each 1ml of gentamicin antibiotic) middle progress screening purifying, culture 10 days or so is carried out under 23 DEG C of half-lights, pure Huang is obtained
Color meat rod bacterium strain.
5) ordinary culture medium is adapted to：The yellow meat rod bacterium strain transfer by antibiotic-screening to common PDA culture medium
In, culture 10 days or so is carried out under 23-25 DEG C of half-light, the yellow meat rod bacterium strain grown on ordinary culture medium is obtained.
Claims (3)
1. a kind of isolation and purification method of yellow meat rod bacterium strain, including：
(1) collection is taken from the yellow meat rod bacterium in field liver moss clump, and surface residues is handled, pending strain point
From；(2) 16 sporangiums are taken in the middle part of yellow meat rod bacterium, be inoculated in the special culture medium prepared and sterilized in advance, at 20-23 DEG C
Culture 10-12 days is carried out under half-light, yellow meat rod bacterium strain is obtained；Wherein, the composition of special culture medium is：Potato 200g, Portugal
Grape sugar 10g, lactose 10g, peptone 10g, yeast extract 10g, potassium chloride 0.05g, sodium sulphate 0.15g, dipotassium hydrogen phosphate 0.5g, chlorine
Change calcium 0.025g, ammonium di-hydrogen phosphate 0.25g, 2% liquor ferri trichloridi 1.2ml, agar 25g, vitamin B1100mg, brewer's wort
15g, distilled water 1000ml；
(3) by yellow meat rod bacterium strain transfer into the culture medium with antibiotic, culture 10- is carried out under 23-25 DEG C of half-light
12 days, obtain the yellow meat rod bacterium strain by antibiotic-screening；Wherein, the composition of the culture medium with antibiotic is：Potato
200g, peptone 10g, yeast extract 10g, glucose 20g, agar 20g, sterilizing be separately added into 1% streptomysin, 1% penicillin,
1% each 1ml of gentamicin antibiotic；
(4) the yellow meat rod bacterium strain transfer for obtaining step (3) is cultivated into PDA culture medium under 20-23 DEG C of half-light
10-12 days, you can.
2. a kind of isolation and purification method of yellow meat rod bacterium strain according to claim 1, it is characterised in that：The step
(1) processing that carried out to surface residues in includes clearing up, sterilizes, dries.
3. a kind of isolation and purification method of yellow meat rod bacterium strain according to claim 1, it is characterised in that：The step
(2) preparation steps of the special culture medium in are：Potato filtering to boil water, adds after agar melts and once adds the albumen melted
Peptone, yeast extract, sequentially add other raw materials, are settled to 1000ml until completely dissolved, are sterilized after packing test tube standby.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710550010.6A CN107287126A (en) | 2017-07-07 | 2017-07-07 | A kind of isolation and purification method of yellow meat rod bacterium strain |
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| CN201710550010.6A CN107287126A (en) | 2017-07-07 | 2017-07-07 | A kind of isolation and purification method of yellow meat rod bacterium strain |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117999A (en) * | 2017-11-10 | 2018-06-05 | 上海市农业科学院 | A kind of preparation method of magic mushroom spot pleat mushroom strains |
| CN109055230A (en) * | 2018-07-05 | 2018-12-21 | 上海市农业科学院 | A kind of preparation method of rare medicinal CNNS's Marasmius wild-type strain |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104640875A (en) * | 2012-09-18 | 2015-05-20 | 维也纳技术大学 | Modified fungal cell |
| CN106834134A (en) * | 2016-12-26 | 2017-06-13 | 上海市农业科学院 | The isolation and purification method of concomitance bacterium Xylaria sp. fungus in a kind of black ear |
-
2017
- 2017-07-07 CN CN201710550010.6A patent/CN107287126A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104640875A (en) * | 2012-09-18 | 2015-05-20 | 维也纳技术大学 | Modified fungal cell |
| CN106834134A (en) * | 2016-12-26 | 2017-06-13 | 上海市农业科学院 | The isolation and purification method of concomitance bacterium Xylaria sp. fungus in a kind of black ear |
Non-Patent Citations (2)
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| SUNG YEOL LIM ET AL.: "Entomopathogenicity of Simplicillium lanosoniveum Isolated in Korea", 《MYCOBIOLOGY》 * |
| 戴水莲 等: "PDA中加入抗菌素的抗菌作用试验", 《食用菌》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117999A (en) * | 2017-11-10 | 2018-06-05 | 上海市农业科学院 | A kind of preparation method of magic mushroom spot pleat mushroom strains |
| CN108117999B (en) * | 2017-11-10 | 2021-06-22 | 上海市农业科学院 | Method for obtaining phomopsis mushroom varix mushroom strain |
| CN109055230A (en) * | 2018-07-05 | 2018-12-21 | 上海市农业科学院 | A kind of preparation method of rare medicinal CNNS's Marasmius wild-type strain |
| CN109055230B (en) * | 2018-07-05 | 2021-07-13 | 上海市农业科学院 | Method for obtaining wild strain of rare medicinal Marasmius androsaceus |
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Application publication date: 20171024 |