CN107271623A - Beer potableness evaluation method based on human body satiety - Google Patents

Beer potableness evaluation method based on human body satiety Download PDF

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Publication number
CN107271623A
CN107271623A CN201710623895.8A CN201710623895A CN107271623A CN 107271623 A CN107271623 A CN 107271623A CN 201710623895 A CN201710623895 A CN 201710623895A CN 107271623 A CN107271623 A CN 107271623A
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mouse
beer
satiety
human body
gastric emptying
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Chinese (zh)
Inventor
董建军
余俊红
胡淑敏
尹花
黄淑霞
刘佳
张翠
黄树丽
杨梅
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Tsingtao Brewery Co Ltd
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Tsingtao Brewery Co Ltd
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Priority to CN201710623895.8A priority Critical patent/CN107271623A/en
Publication of CN107271623A publication Critical patent/CN107271623A/en
Priority to CN201810825643.8A priority patent/CN108872501B/en
Priority to CN201810826381.7A priority patent/CN108776202B/en
Priority to CN201810825613.7A priority patent/CN108776201B/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02Food
    • G01N33/14Beverages
    • G01N33/146Beverages containing alcohol

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  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Alcoholic Beverages (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides a kind of beer potableness evaluation method based on human body satiety, belong to beer field.The evaluation method can accurately, efficiently, quickly evaluate human body satiety, required instrument and equipment is simple, and testing cost is relatively low, and data stability is preferable.The evaluation method comprises the following steps:Beer, test and calculating mouse gastric emptying rate are gavaged after mouse is weighed, the average gastric emptying rate of mouse is calculated, human body satiety is evaluated according to the average gastric emptying rate of mouse, according to human body satiety evaluating beer potableness;Evaluation criterion is:As the average gastric emptying rate > 70% of mouse, human body satiety is evaluated as no satiety, and beer potableness is strong;When the average gastric emptying rate of mouse for 60% 70% when, human body satiety is evaluated as slightly satiety, and beer potableness is medium;As the average gastric emptying rate < 60% of mouse, human body satiety is evaluated as satiety substantially, and beer potableness is weak.

Description

Beer potableness evaluation method based on human body satiety
Technical field
The present invention relates to beer field, more particularly to a kind of beer potableness evaluation method based on human body satiety.
Background technology
Potableness is related to quality and the sale of product, is always the research field that beverage industry is paid close attention to.But due to Human body drinks the high complexity of behavior, and the flow of research in the field is slow.The human body behavior of drinking is a complicated physiology heart Social action is managed, is mainly influenceed by psychology and physiology both sides.For the behavior of drinking of beer, mainly drunk in terms of physiology The shadow that satiety, gastric emptying and the beer component caused afterwards is caused by the biochemical metabolism of blood of human body to brain and body etc. Ring etc..
To improve potableness of the beer in terms of physiology, emphasis is to eliminate the satiety in beer drinking process, improves stomach Rate of evacuation, accelerates the metabolism of beer, so as to improve the potableness of beer.Therefore, to improve beer potableness, beer was drunk Human body satiety is one of very important potableness evaluation index in journey.
The current country there is no the beer potableness evaluation method for human body satiety index in beer drinking process.In state Outside, the beer potableness evaluation based on human body satiety is, by organizing human body to drink experiment, to be seen using ultrasonic wave or gastroscope Examine beer and drink rear satiety degree, so that judge that the potableness of beer is strong and weak, but there is notable defect in the above method.
First, the above method is needed to expend a large amount of human and material resources, and cost is high, and time-consuming, and whole evaluation procedure operation is compared It is complicated;Secondly, the above method is larger by individual and ambient influnence, and data have larger fluctuation, it is easy to cause the deviation of experiment, Cause evaluation result unstable;Again, the above method is required for being tested by human body, larger to body effect, easily Produce side reaction.Therefore, the current country is substantially at blank stage, and external method defect is more, both at home and abroad without fast It is fast, effective, and to the evaluation method of the less beer potableness based on human body satiety of body effect.
The content of the invention
It is an object of the invention to provide a kind of beer potableness evaluation method based on human body satiety, can accurately, Efficiently, quickly evaluate human body satiety, and then the potableness of evaluating beer, required instrument and equipment is simple, testing cost compared with Low, data stability is preferable.
The technical scheme is that:Beer potableness evaluation method based on human body satiety, comprises the following steps:
Beer, test and calculating mouse gastric emptying rate are gavaged after mouse is weighed, the average gastric emptying rate of mouse is calculated, according to The average gastric emptying rate of mouse evaluates human body satiety, according to human body satiety evaluating beer potableness;
Evaluation criterion is:As the average gastric emptying rate > 70% of mouse, human body satiety is evaluated as no satiety, and beer can be drunk Property is strong;When the average gastric emptying rate of mouse is 60%-70%, human body satiety is evaluated as in slightly satiety, beer potableness Deng;As the average gastric emptying rate < 60% of mouse, human body satiety is evaluated as satiety substantially, and beer potableness is weak.
As preferred technical scheme, it is described mouse is weighed after gavage beer, test and calculate mouse gastric emptying rate Step includes:
Insta-Char is prepared, by beer and Insta-Char according to 4:1 ratio is well mixed that beer is suspended Liquid;
Mouse is weighed, beer suspension oral gavage is carried out to mouse according to 0.5-0.6ml/25g dosage, 10-20min is gavaged Afterwards, the gastric emptying rate of mouse is calculated.
As preferred technical scheme, the preparation of the Insta-Char comprises the following steps:
8-10g gum arabic powders are weighed, 80-100ml distilled water is added, boils to transparent;
8-10g active carbon powders are added, boils, obtains Insta-Char.
As optional technical scheme, add that 8-10g activated carbon powders are last to be boiled three times.
Include as preferred technical scheme, the step of the gastric emptying rate for calculating mouse:
Gavage after 10-20min, mouse cervical dislocation is put to death, open abdominal cavity, be rapidly separated stomach and intestine, observation activated carbon Course;
The gastric emptying rate of mouse is calculated, calculation formula is:Gastric emptying rate=pylorus is to distance/pylorus of carbon powder to ileocecus Distance × 100%.
As optional technical scheme, mouse is adult Kunming white mouse, and female is equal with male quantity.
As optional technical scheme, the quantity of adult Kunming white mouse is 20, in advance observation 1 week, is removed clinical There is abnormal person.
Compared with prior art, the beneficial effects of the present invention are:
1st, instrument and equipment is simple needed for evaluation method of the invention, and gastroscope and ultrasonic wave be all in external evaluation method Medical supply is needed, not only instrument cost is higher, and higher is required to experimental enviroment, significantly increase the evaluation of beer potableness Difficulty;
2nd, the testing cost of evaluation method of the invention is relatively low, only needs experimental animal, and existing literature procedure is needed Volunteer is recruited, the time is long, organizes complexity, and cost is higher;
3rd, evaluation method of the invention is easily controlled to experimental animal, environment etc., and the data stability of acquisition preferably, and shows Because human body individual difference is larger in some document reports, and by environment, diet, emotion influence, experiment uniformity is poor;
4th, evaluation method of the invention experiment is time-consuming shorter, simply, efficiently, multiple samples can be analyzed simultaneously;And it is existing Literature procedure, time-consuming longer, the same time can only analyze a sample.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation Example only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model that the present invention is protected Enclose.
The embodiments of the invention provide a kind of beer potableness evaluation method based on human body satiety, including following step Suddenly:
Beer, test and calculating mouse gastric emptying rate are gavaged after mouse is weighed, the average gastric emptying rate of mouse is calculated, according to The average gastric emptying rate of mouse evaluates human body satiety, according to human body satiety evaluating beer potableness;
Evaluation criterion is:As the average gastric emptying rate > 70% of mouse, human body satiety is evaluated as no satiety, and beer can be drunk Property is strong;When the average gastric emptying rate of mouse is 60%-70%, human body satiety is evaluated as in slightly satiety, beer potableness Deng;As the average gastric emptying rate < 60% of mouse, human body satiety is evaluated as satiety substantially, and beer potableness is weak.
Average gastric emptying rate of the above-mentioned evaluation method based on mouse evaluates human body satiety, without large scale equipment tester Bodies, a large amount of volunteers need not be recruited, do not influenceed by human body individual difference, cost is low, can quick, efficient evaluating beer drink During human body satiety, and then determine beer potableness power, can for beer product test significant data branch be provided Hold.It is understood that during actual evaluation, when the average gastric emptying rate of mouse is critical numerical value, test of many times can be passed through Judged according to the multiple measured value of the average gastric emptying rate of mouse, but generally, when the average gastric emptying of mouse Rate is 60%, 62%, 64%, 66%, 68%, when 70%, and human body satiety evaluation is slightly satiety, and beer potableness is commented Valency is medium.
In a preferred embodiment, it is described mouse is weighed after gavage beer, test and the step for calculating mouse gastric emptying rate Suddenly include:Insta-Char is prepared, by beer and Insta-Char according to 4:1 ratio is well mixed that beer is suspended Liquid;Mouse is weighed, beer suspension oral gavage is carried out to mouse according to 0.5-0.6ml/25g dosage, gavaged after 10-20min, is counted Calculate the gastric emptying rate of mouse.In the present embodiment, label activated carbon is used to calculate the gastric emptying rate of mouse for medium, specifically , by process of the test, the ratio of beer and Insta-Char is 4:Effect preferably, neither influences the property of beer, again when 1 Appropriate activated carbon be ensure that as calculating medium, but those skilled in the art can be according to the concentration and activated carbon of beer The concentration of suspension is simply adjusted to above-mentioned mixed proportion.Further, since the given low of mouse is 0.5-0.6ml/ 25g, equivalent to gavaging for 800ml-960ml beer/50Kg human bodies, is identical with human trial, specific mouse stomach agent It can be 0.5ml/25g to measure, 0.52ml/25g, 0.54ml/25g, 0.56ml/25g, 0.58ml/25g, 0.6ml/25g etc..
In a preferred embodiment, Insta-Char is prepared to comprise the following steps:8-10g gum arabic powders are weighed, 80-100ml distilled water is added, is boiled to transparent;8-10g active carbon powders are added, boils, obtains Insta-Char.In this reality Apply in example, gum arabic can wrap up activated carbon, the course of apparent reflection activated carbon in mouse stomach and intestine, on The concentration for stating the Insta-Char of method preparation coordinates the gastric emptying rate for being beneficial to test mouse with beer.
In one preferred embodiment of above-mentioned evaluation method, calculate mouse gastric emptying rate the step of include:Gavage 10- After 20min, mouse cervical dislocation is put to death, abdominal cavity is opened, stomach and intestine are rapidly separated, the course of activated carbon is observed;Calculate small The gastric emptying rate of mouse, calculation formula is:Gastric emptying rate=pylorus to carbon powder distance/pylorus to ileocecus distance × 100%. The present embodiment can intuitively observe the gastric emptying route of beer by the observation of the course to activated carbon, meanwhile, by upper State formula and calculate the gastric emptying rate that obtained mouse gastric emptying rate accurately reflects beer.
In an alternative embodiment, add that 8-10g activated carbon powders are last boils three times.The above-mentioned basic object for boiling operation It is sterilization and eliminates the influence of possible beer gastrointestinal reaction, adds last repeatedly boil of activated carbon powder and ensure that and ensure for trying out The order of accuarcy of the gastrointestinal reaction of product.But it is understood that, number of times is specifically boiled according to can be slightly the need for experiment Adjustment.
In an alternative embodiment, mouse is adult Kunming white mouse, and female is equal with male quantity.The present embodiment In, adult kunming mouse is optimal subjects, is to be kept with human body sex difference is caused in beer drinking process difference Correspondence, takes the female and male of equivalent, it is ensured that the reliability of experiment respectively for the mouse of examination.
In an alternative embodiment, the quantity of adult Kunming white mouse is 20, in advance observation 1 week, and removing clinic has Abnormal person.The quantity of the present embodiment can be with the accuracy of guarantee test, it is to be understood that above-mentioned quantity can increase as needed Plus or reduce the accurate of even double guarantee test, the accuracy of experiment can be improved by removing clinical exception person.
The beer potableness based on human body satiety provided to become apparent from introducing the embodiment of the present invention in detail is commented Valency method, is illustrated below with reference to specific embodiment.
Embodiment 1
Different product mouse gastric emptying rate drinks experiment with human body
Tissue human body drinks experiment, and subjects are 20 people, and 10 male 10 female are drunk into phase according to phase same rate (15ml/min) Often drunk in the beer (A, B, C) of the water of same content (500-2000ml) and 3 different categories, drinking process and finish 500ml (1 Bottle) record whether have satiety and degree afterwards, record produces the amount of drinking during satiety first.Drink during satiety is produced first Consumption is bigger, and the satiety of representative sample is lighter, and everyone is ranked up the satiety degree to 4 samples.Comprehensive 20 people Satiety ranking results, the satiety intensity to 4 samples carries out evaluation sequence, and then determines the power of beer potableness.
Mouse gastric emptying rate analysis is carried out to the beer sample of water and 3 different categories simultaneously:Adult Kunming white mouse 80, male and female half and half, observation 1 week before experiment, clinic has abnormal person's elimination test.
10g gum arabic powders are weighed, 100ml distilled water is added, boils to transparent;10g active carbon powders are added, are boiled Three times, obtain Insta-Char;
By above-mentioned water or beer sample with Insta-Char according to 4:1 ratio is well mixed to obtain aqueous suspension or beer Suspension;
Mouse is weighed, beer is carried out to mouse according to 0.5ml/25g (equivalent to 800ml beer/50Kg human bodies) dosage Wine suspension oral gavage, is gavaged after 20min, and mouse cervical dislocation is put to death, and opens abdominal cavity, is rapidly separated stomach and intestine, observes activated carbon Course;
The gastric emptying rate of mouse is calculated, calculation formula is:Gastric emptying rate=pylorus is to distance/pylorus of carbon powder to ileocecus Distance × 100%.Mouse gastric emptying rate is averaged, it is as a result as shown in table 1 below.
Table 1:Volunteer's satiety of water and three beer samples is evaluated and mouse gastric emptying rate Comparative result
Sample Volunteer evaluates Satiety sorts The average gastric emptying rate of mouse
Water Slightly satiety 2 65%
A Satiety 3 55%
B There is no satiety 1 75%
C Drinking the later stage has satiety (women) 4 Female 50%, hero 60%
As it can be seen from table 1 mouse gastric emptying rate is consistent with the satiety result in human body drinking process.Absolutely prove, Human body satiety that can accurately and efficiently in evaluating beer drinking process using the evaluation method of the present invention, so that it is determined that beer Wine potableness is strong and weak, and scientific basis is provided to improve beer potableness.
Embodiment 2
New product development evaluation test
In new product development, to sample progress human body drinks experiment, concrete operations before process modification and after process modification Method be the same as Example, record human body evaluation and satiety sequence.Simultaneously 2 samples are carried out with the calculating of mouse gastric emptying rate, is surveyed Method for testing be the same as Example 1, it is as a result as shown in table 2 below.
Table 2:Volunteer's satiety before and after product improvement is evaluated and mouse gastric emptying rate Comparative result
Sample Volunteer evaluates Satiety sorts The average gastric emptying rate of mouse
Before product improvement Satiety is serious after two bottles, and potableness declines 2 50%
After product improvement Continuous drink has no satiety after three or four tanks, potableness is strong 1 82%
The average gastric emptying rate of mouse that can be seen that the present invention by the interpretation of result of table 2 is highly consistent with satiety , the average gastric emptying rate of mouse can highly ensure beer satiety evaluation result, when the average gastric emptying rate of mouse is relatively low, Product human body satiety is stronger, and the potableness of product is weaker;When the average gastric emptying rate of mouse is higher, product human body satiety compared with Gently, the potableness of product is stronger, therefore, and evaluation method of the invention can be efficiently used for the potableness evaluation and improvement of product In.

Claims (7)

1. the beer potableness evaluation method based on human body satiety, it is characterised in that comprise the following steps:
Beer, test and calculating mouse gastric emptying rate are gavaged after mouse is weighed, the average gastric emptying rate of mouse is calculated, according to mouse Average gastric emptying rate evaluates human body satiety, according to human body satiety evaluating beer potableness;
Evaluation criterion is:As the average gastric emptying rate > 70% of mouse, human body satiety is evaluated as no satiety, beer potableness By force;When the average gastric emptying rate of mouse is 60%-70%, human body satiety is evaluated as slightly satiety, and beer potableness is medium; As the average gastric emptying rate < 60% of mouse, human body satiety is evaluated as satiety substantially, and beer potableness is weak.
2. the beer potableness evaluation method according to claim 1 based on human body satiety, it is characterised in that described to incite somebody to action Mouse gavages beer, test and calculating mouse gastric emptying rate after weighing the step of includes:
Insta-Char is prepared, by beer and Insta-Char according to 4:1 ratio is well mixed to obtain beer suspension;
Mouse is weighed, beer suspension oral gavage is carried out to mouse according to 0.5-0.6ml/25g dosage, gavaged after 10-20min, Calculate the gastric emptying rate of mouse.
3. the beer potableness evaluation method according to claim 2 based on human body satiety, it is characterised in that the system Standby Insta-Char comprises the following steps:
8-10g gum arabic powders are weighed, 80-100ml distilled water is added, boils to transparent;
8-10g active carbon powders are added, boils, obtains Insta-Char.
4. the beer potableness evaluation method according to claim 3 based on human body satiety, it is characterised in that add 8- 10g activated carbon powders are last to be boiled three times.
5. the beer potableness evaluation method according to claim 2 based on human body satiety, it is characterised in that calculate small The step of gastric emptying rate of mouse, includes:
Gavage after 10-20min, mouse cervical dislocation is put to death, open abdominal cavity, be rapidly separated stomach and intestine, observe the traveling of activated carbon Route;
The gastric emptying rate of mouse is calculated, calculation formula is:Gastric emptying rate=pylorus to distance/pylorus of carbon powder to ileocecus away from From × 100%.
6. the beer potableness evaluation method according to claim 1 based on human body satiety, it is characterised in that mouse is Adult Kunming white mouse, female is equal with male quantity.
7. the beer potableness evaluation method according to claim 6 based on human body satiety, it is characterised in that adult elder brother The quantity of bright system small white mouse is 20, in advance observation 1 week, and removing clinic has abnormal person.
CN201710623895.8A 2017-07-27 2017-07-27 Beer potableness evaluation method based on human body satiety Pending CN107271623A (en)

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CN201710623895.8A CN107271623A (en) 2017-07-27 2017-07-27 Beer potableness evaluation method based on human body satiety
CN201810825643.8A CN108872501B (en) 2017-07-27 2018-07-25 Beer and beer malt potability evaluation method based on human body satiety
CN201810826381.7A CN108776202B (en) 2017-07-27 2018-07-25 Beer and hop potability evaluation method based on human body satiety
CN201810825613.7A CN108776201B (en) 2017-07-27 2018-07-25 Drinkability evaluation method of beer and beer yeast strain based on human satiety

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CN201810825613.7A Active CN108776201B (en) 2017-07-27 2018-07-25 Drinkability evaluation method of beer and beer yeast strain based on human satiety
CN201810825643.8A Active CN108872501B (en) 2017-07-27 2018-07-25 Beer and beer malt potability evaluation method based on human body satiety
CN201810826381.7A Active CN108776202B (en) 2017-07-27 2018-07-25 Beer and hop potability evaluation method based on human body satiety

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CN201810825643.8A Active CN108872501B (en) 2017-07-27 2018-07-25 Beer and beer malt potability evaluation method based on human body satiety
CN201810826381.7A Active CN108776202B (en) 2017-07-27 2018-07-25 Beer and hop potability evaluation method based on human body satiety

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107908924A (en) * 2017-11-19 2018-04-13 中国食品发酵工业研究院 A kind of method for evaluating excitement degree after drinks is drunk
CN108776203A (en) * 2018-07-25 2018-11-09 青岛啤酒股份有限公司 Influence the beer component screening method of human body satiety
CN108893215A (en) * 2018-07-25 2018-11-27 青岛啤酒股份有限公司 Human body drinks the nablab and preparation method thereof of no satiety

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2763337B1 (en) * 1997-05-13 1999-08-20 Sanofi Sa NOVEL TRIAZOLE DERIVATIVES, A PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM
CN101024802B (en) * 2006-02-17 2010-05-12 阴胜元 Pine-juice beer and its brewing method
CN101875899B (en) * 2010-05-07 2013-02-27 青岛啤酒股份有限公司 Alcohol-free beer and preparation method thereof
CN105087204B (en) * 2015-09-21 2017-10-31 青岛啤酒股份有限公司 Evaluate the method that different hops brewage performance in Lager brewings
CN106769366B (en) * 2016-11-22 2019-02-05 青岛啤酒股份有限公司 That evaluates a limited number of hops hardship sense quality judges sample preparation methods

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107908924A (en) * 2017-11-19 2018-04-13 中国食品发酵工业研究院 A kind of method for evaluating excitement degree after drinks is drunk
CN108776203A (en) * 2018-07-25 2018-11-09 青岛啤酒股份有限公司 Influence the beer component screening method of human body satiety
CN108893215A (en) * 2018-07-25 2018-11-27 青岛啤酒股份有限公司 Human body drinks the nablab and preparation method thereof of no satiety

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CN108776201A (en) 2018-11-09
CN108872501B (en) 2021-01-22
CN108872501A (en) 2018-11-23
CN108776201B (en) 2021-01-22
CN108776202B (en) 2021-01-22

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