CN107267488A - A kind of efficient bromelain preparation method - Google Patents
A kind of efficient bromelain preparation method Download PDFInfo
- Publication number
- CN107267488A CN107267488A CN201710715701.7A CN201710715701A CN107267488A CN 107267488 A CN107267488 A CN 107267488A CN 201710715701 A CN201710715701 A CN 201710715701A CN 107267488 A CN107267488 A CN 107267488A
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- pineapple
- bromelain
- juice
- microfiltration
- efficient
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22033—Fruit bromelain (3.4.22.33), i.e. juice bromelain
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a kind of efficient bromelain preparation method, comprise the following steps：Step 1: cleaning treatment；Step 2: crushing；Step 3: squeezing the juice；Step 4: two-stage centrifugal；Step 5: microfiltration of ceramic membrane；Step 6: being concentrated by ultrafiltration；Step 7: drying.The present invention improves the purity of Clarification of Pineapple Juice, reduces micro-filtration pressure by carrying out two-stage centrifugal processing to the pineapple juice squeezed.Handled by using microfiltration of ceramic membrane, not only realize the high retention under the physical state to viable bacteria body, while isolating clarity very high enzyme downstream clear liquid, reduce the produce load of downstream concentration technique.By using ultrafiltration concentration, partial pigment and foreign protein are eliminated, product quality and stability is improved, carried out at normal temperatures while being concentrated by ultrafiltration, enzyme activity is not lost, and improves the yield of bromelain.
Description
Technical field
The production method of present invention design phytoenzyme, more particularly to a kind of efficient bromelain preparation method.
Background technology
Bromelain is that one kind is come from pineapple sarcocarp, stem, leaf at extraction, by refined, purification, concentration, freeze-drying
A kind of natural plant protein enzyme obtained afterwards, with the very strong ability for decomposing fibrin and blood clot, while can also promote
Drug absorption and the growth for suppressing tumour cell.
At present, the main production process of bromelain has physical extracting method and chemical manufacturing process, with membrane technology
Development, concentration UF membrane extracts bromelain and has been obtained for extensive use, but because pineapple juice contains in separation process
Impurity is more, and required supercharging pump pressure is larger, and is easily damaged concentration film, reduces the service life of concentration film.
The content of the invention
, can be with high efficiency extraction bromelain it is an object of the invention to provide a kind of efficient bromelain preparation method
The method of enzyme, it is ensured that the quality and enzymatic activity of the bromelain of extraction.
A kind of production method of bromelain of technical solution of the present invention, comprises the following steps：
Step 1: cleaning treatment, by peeling pineapple, places stand-by after cleaning up；
Step 2: crushing, the pineapple of wash clean is put into pulverizer and is stirred with 200r/min speed, obtains powdered
Pineapple；
Step 3: squeezing the juice, putting the pineapple after crushing into juice extractor is made pineapple juice suspension；
Step 4: two-stage centrifugal, puts pineapple juice suspension into centrifuge and carries out first-stage centrifugal processing, stand after a period of time again
Secondary progress secondary centrifuging processing, goes the removal of impurity, the pineapple juice clarified；
Step 5: microfiltration of ceramic membrane, is passed through ceramic membrane separation device 0.2mpa by Clarification of Pineapple Juice and carries out microfiltration processing, obtain
The enzyme downstream stillness of night；
Step 6: be concentrated by ultrafiltration, take enzyme downstream clear liquid by feed be pumped into milipore filter concentrator under 0.3mpa pressure it is dense
Contracting is handled, and is removed partial pigment and foreign protein, is obtained bromelain concentrate；
Step 7: drying, concentrate is subjected to vacuum drying treatment, bromelain is produced.
It is preferred that, in the step 2, the mixing speed of pulverizer is 200r/min.
It is preferred that, in the step 4, centrifuge speed is 220 r/min, each centrifugation time 30 minutes.
It is preferred that, in the step 6, milipore filter selection makrolon, molecular cut off is 25000.
It is preferred that, the pressure in the step 5 and step 6 is provided by booster pump.
It is preferred that, microfiltration of ceramic membrane equipment is connected with concentrator in step 6 by pipeline in the step 5.
The technology of the present invention beneficial effect：
A kind of efficient bromelain preparation method of the present invention, by carrying out two-stage centrifugal processing to the pineapple juice squeezed,
The purity of Clarification of Pineapple Juice is improved, micro-filtration pressure is reduced.Handle, not only realized to viable bacteria by using microfiltration of ceramic membrane
High retention under the physical state of body, while isolating clarity very high enzyme downstream clear liquid, reduces downstream concentration technique
Produce load.By using ultrafiltration concentration, partial pigment and foreign protein are eliminated, product quality and stability is improved, simultaneously
Ultrafiltration concentration is carried out at normal temperatures, and enzyme activity is not lost, and improves the yield of bromelain.
Brief description of the drawings
Fig. 1 is a kind of process chart of efficient bromelain preparation method of the present invention.
Embodiment
For ease of skilled artisan understands that technical solution of the present invention, in conjunction with Figure of description to the technology of the present invention side
Case is described further.
As shown in figure 1, the efficient bromelain preparation method of the present invention, comprises the following steps：Pineapple raw material it is clear
Wash processing, crush, squeeze the juice, two-stage centrifugal, microfiltration of ceramic membrane, ultrafiltration concentration, drying, finished product.
Specifically：Pineapple raw material is removed the peel, put into after being cleaned up in rinse bath, with pulverizer with 200r/min stirring
Speed carries out pulverization process to pineapple.Pineapple after crushing, which is transported to juice extractor by conveyer belt and squeeze the juice, to be obtained pineapple and suspends
Liquid.Pineapple suspension is put into the clarification pineapple centrifuged for the first time after centrifuge is centrifuged 30 minutes with 220 r/min rotating speed
Juice, is placed again into centrifuge and carries out secondary centrifuging processing with same speed and time, obtain after Clarification of Pineapple Juice is stood into 5 minutes
To final Clarification of Pineapple Juice.Clarification of Pineapple Juice is passed through microfiltration of ceramic membrane equipment, starts booster pump and micro- mistake is carried out with 0.2mpa
Filter is handled, and removes and clarity very high enzyme downstream clear liquid is obtained after partial pigment and foreign protein.Gained enzyme downstream clear liquid directly leads to
Cross Pipeline transport and carry out concentrating and separating to film device is concentrated by ultrafiltration, obtain bromelain concentrate.Bromelain is concentrated
Liquid is put into vacuum drying chamber dried 10 hours with 60 DEG C of temperature after obtain solid-state bromelain.
Invention is exemplarily described above in conjunction with accompanying drawing for technical solution of the present invention, it is clear that the present invention is implemented
It is not subject to the restrictions described above, as long as the various unsubstantialities for employing inventive concept and technical scheme of the present invention progress change
Enter, or it is not improved the design of invention and technical scheme are directly applied into other occasions, in protection scope of the present invention
Within.
Claims (3)
1. a kind of efficient bromelain preparation method, it is characterised in that comprise the following steps：
Step 1: cleaning treatment, by peeling pineapple, places stand-by after cleaning up；
Step 2: crushing, the pineapple of wash clean is put into pulverizer and is stirred with 200r/min speed, obtains powdered
Pineapple；The mixing speed of pulverizer is 200r/min；
Step 3: squeezing the juice, putting the pineapple after crushing into juice extractor is made pineapple juice suspension；
Step 4: two-stage centrifugal, puts pineapple juice suspension into centrifuge and carries out first-stage centrifugal processing, stand after a period of time again
Secondary progress secondary centrifuging processing, goes the removal of impurity, the pineapple juice clarified；Centrifuge speed is 220 r/min, when centrifuging every time
Between 30 minutes；
Step 5: microfiltration of ceramic membrane, is passed through ceramic membrane separation device 0.2mpa by Clarification of Pineapple Juice and carries out microfiltration processing, obtain
The enzyme downstream stillness of night；
Step 6: be concentrated by ultrafiltration, take enzyme downstream clear liquid by feed be pumped into milipore filter concentrator under 0.3mpa pressure it is dense
Contracting is handled, and is removed partial pigment and foreign protein, is obtained bromelain concentrate；Milipore filter selects makrolon, retains molecule
Measure as 25000；
Step 7: drying, concentrate is subjected to vacuum drying treatment, bromelain is produced.
2. a kind of efficient bromelain preparation method according to claim 1, it is characterised in that the step 5 and
Pressure in step 6 is provided by booster pump.
3. a kind of efficient bromelain preparation method according to claim 1, it is characterised in that in the step 5
Microfiltration of ceramic membrane equipment is connected with concentrator in step 6 by pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710715701.7A CN107267488A (en) | 2017-08-20 | 2017-08-20 | A kind of efficient bromelain preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710715701.7A CN107267488A (en) | 2017-08-20 | 2017-08-20 | A kind of efficient bromelain preparation method |
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| Publication Number | Publication Date |
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| CN107267488A true CN107267488A (en) | 2017-10-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710715701.7A Pending CN107267488A (en) | 2017-08-20 | 2017-08-20 | A kind of efficient bromelain preparation method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1908165A (en) * | 2006-08-03 | 2007-02-07 | 谢丹青 | Novel extraction method of bromelain |
| CN101319208A (en) * | 2008-04-08 | 2008-12-10 | 徐闻县美仑生物制品有限公司 | Method for preparation of bromelin |
| CN104611316A (en) * | 2015-02-13 | 2015-05-13 | 广西壮族自治区分析测试研究中心 | Method for extracting bromelin |
| CN106282144A (en) * | 2016-09-23 | 2017-01-04 | 合肥信达膜科技有限公司 | A kind of bromelain production method |
-
2017
- 2017-08-20 CN CN201710715701.7A patent/CN107267488A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1908165A (en) * | 2006-08-03 | 2007-02-07 | 谢丹青 | Novel extraction method of bromelain |
| CN101319208A (en) * | 2008-04-08 | 2008-12-10 | 徐闻县美仑生物制品有限公司 | Method for preparation of bromelin |
| CN104611316A (en) * | 2015-02-13 | 2015-05-13 | 广西壮族自治区分析测试研究中心 | Method for extracting bromelin |
| CN106282144A (en) * | 2016-09-23 | 2017-01-04 | 合肥信达膜科技有限公司 | A kind of bromelain production method |
Non-Patent Citations (3)
| Title |
|---|
| 唐梅 等: "响应面法优化超滤提取菠萝蛋白酶工艺条件", 《食品研究与开发》 * |
| 谢希贤 等: "采用膜分离技术高效分离提取L-亮氨酸", 《食品与发酵工业》 * |
| 韩志萍 等: "超滤分离纯化菠萝蛋白酶的研究", 《食品工业科技》 * |
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