CN107253871A - A kind of rape increases oil foliar fertilizer and its application - Google Patents
A kind of rape increases oil foliar fertilizer and its application Download PDFInfo
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- CN107253871A CN107253871A CN201710352529.3A CN201710352529A CN107253871A CN 107253871 A CN107253871 A CN 107253871A CN 201710352529 A CN201710352529 A CN 201710352529A CN 107253871 A CN107253871 A CN 107253871A
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- agent
- rape
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- oil
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Dentistry (AREA)
- Soil Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Increase oil foliar fertilizer and its application in rape seed oil content is improved the invention discloses a kind of rape, belong to technical field of fertilizers.The foliar fertilizer include the A agent that initial bloom stage applies and start at the rape whole florescence after the B agent applied for the 10th~20 day, the active ingredient of the A agent is paclobutrazol, abscisic acid and boric acid, and concentration is respectively 0.1~1.0g/L, 0.01~1mg/L, 10~30g/L；The active ingredient of the B agent is paclobutrazol and abscisic acid, and concentration is respectively 0.05~0.5g/L, 0.5~10mg/L.The present invention is directed to the different characteristics of rape seed growth and development process, and respectively in the initial bloom stage foliar fertilizer different with whole Spraying in the flowering stage active principle, paclobutrazol, abscisic acid hinder the decomposition of seed development stage grease, improve seed oil content；Borax composition improves unit area grain yield, and then increases total oil production of unit area.
Description
Technical field
The present invention relates to technical field of fertilizers, and in particular to a kind of rape increases oil foliar fertilizer and its improving rape seed
Application in oil content.
Background technology
Cruciferae Brassica genus oil crops (being commonly called as rape) are one of main winter crops in China Yangtze river basin, in China
Long-term about 1.2 hundred million mu of cultivated area；Rapeseed oil occupies domestic edible vegetable oil total amount more than 55%, is the big edible oil of China first
Source.Although China and the country (area) such as Canada, Europe, India, Australia are several main rape producing regions greatly in the world,
But it is annual close in 30,000,000 tons of vegetable oil aggregate consumption in China, only 1/3 or so is self-supporting (domestic), 2/3
Left and right dependence on import (data source：Chinese edible oil industry research report http in 2014://www.doc88.com/p-
9009456345166.html).The import for reducing edible vegetable oil is the great demand for ensureing national grain and oil supply security.China
Rape-seed production distinct issues are that unit area oil production (oil-producing efficiency) is not high, and wherein one of limiting factor is exactly domestic vegetable seed
Oil content it is relatively low, most of winter rape is main to carry variety seeds oil content 41% or so, than Germany, the kind of canola kind
Sub- oil content (absolute value) is low 3-5 percentage points, and this directly contributes domestic rapeseed, and competitiveness is not on oil product processing market
Such as the situation of import vegetable seed.Therefore, the oil content for greatly improving domestic rapeseed is lifting vegetable seed quality, changes China's vegetable oil
The breach of the present situation of dependence on import.
Grease, protein and starch (polysaccharide) are the main reserve substances of vegetable seeds.Grease is Cruciferae Btassica oil
Expect the target product of crop (being commonly called as rape) seed, it is always one of main breeding objective of rape to improve seed oil content.Plant
The synthesis of grease is divided into three phases：First, acetyl coenzyme A (acetyl-CoA) synthesizes 16-18 carbon fatty acids in plastid；So
Afterwards, aliphatic acid is transported to endoplasmic reticulum, and extension and the desaturation of carbochain are carried out there；Finally, aliphatic acid is processed with glycerine
Into triglyceride (triacylglycerol, abbreviation TAG) (Ohrogge and Browse, 1995；Shamsi et al.,
2010), aliphatic acid is main in vegetable seeds exists in the form of TAG.
In the tissue such as root, blade in arabidopsis and rape, the content of aliphatic acid is very low, but in its seed, glycerine three
The content of fat accounts for 34.6-46% or so (O ' Neill et al., 2003) and 35-50% or so respectively.TAG can be sweet in trigalloyl
Free fatty and glycerine are resolved into the presence of oil and fat enzyme, free fatty will pass through the decomposition of beta oxidation approach quickly
(Graham and Eastmond,2002).The cumulative process of vegetable seeds aliphatic acid is actually the mistake of a dynamic equilibrium
Journey, is on the one hand accumulation, on the other hand synchronous to decompose, if accumulation is more than decomposing, Interior Seed grease just gradually increases；Instead
It, if decomposed more than accumulation, seed grease is gradually decreased.
Past, the effort of people's lifting seed oil content is placed on being effectively synthesized above for increase grease mostly, such as, increase
The substrate of synthetic reaction, suppresses to compete the synthesis of the materials such as storage protein, crude fibre, the pigment of substrate with oil synthesis, or
Promote synthesis and transhipment (the Cahoon and Shanklin, 2000 of intermediate product by transforming key gene；Kim et al.,
2013；Thelen and Ohlrogge,2002).For example, in rape seed overexpression arabidopsis HO-ACCase, make third
Diacid list acyl CoA yield increase, can make seed oil content improve 3-5% (Roesler et al., 1997).People's also handle is carried
The effort of high seed oil content is placed on TAG biosynthesis pathway, and yeast long-chain Sn-2 acyltransferases such as are transferred into rape
And arabidopsis, the TAG in rape and arabidopsis seed can be made to obtain a certain degree of improving (Zou et al., 1997).And for example,
Overexpression diacylglycerol acyltransferase (DGAT), can make the relative amount of grease in seed improve 30-40% (Jako
et al.,2001)。
Because the gene that some enzyme is encoded on overexpression route of synthesis is limited for improving seed oil content effect, then,
There is scholar again and controlled by manipulating transcription factor the metabolism of aliphatic acid, such as, overexpression WRI1 can significantly increase seed
Oil content because WRI1 codings AP2/EREB transcription factors albuminoid (Cernac and Benning, 2004), it can regulate and control
Plant internal carbon source turns to oil synthesis (Ruuska et al., 2002 by glucose；Masaki et al.,2005).Have perhaps
Many transcription factors participate in vegetable seeds embryo development procedure and influence the accumulation of seed oil, suitable according to the time of gene expression
Sequence, is LEC2, LEC1, WRI1, FUS3 and ABI3 respectively, therefore it is considered as to realize seed oil content to manipulate these transcription factors
Important means (the Baud et al., 2009 of raising；Kroj et al.,2003；Pan et al.,2010).
Our research indicate that, in the homeostasis process of seed oil and fat accumulation, the decomposition of reduction grease is also to realize
The important channel that oil content is improved, is current still ignored breach.
The content of the invention
Increase oil foliar fertilizer it is an object of the invention to provide a kind of rape, specifically grown period in rape, with
Specific frequency and concentration are applied, and effectively improve the oil content of rape seed, increase unit area oil production.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of rape increases oil foliar fertilizer, including A agent and B agent,
The active ingredient of the A agent is paclobutrazol, abscisic acid and boric acid, and the wherein concentration of paclobutrazol is 0.1~1.0g/L,
The concentration of abscisic acid is 0.01~1mg/L, and the concentration of boric acid is 10~30g/L；
The active ingredient of the B agent is paclobutrazol and abscisic acid, and the wherein concentration of paclobutrazol is 0.05~0.5g/L, is come off
The concentration of acid is 0.5~10mg/L.
The rape seed stage of development, accumulation and the abatement of grease are a homeostasis processes, and seed maturity process is allusion quotation
The accumulation of type is more than decomposable process, and seed is sprouted, the process of establishing of seedling morphology is that typical decompose is more than cumulative process.This
Invention early-stage Study shows：The active list that grease abatement comes from grease abatement gene reaches, and the expression of grease abatement gene is by one
The regulation and control of serial stream signal (such as gibberellin).Therefore, present invention selection is intervened in the seed growth stage of development, using many
The synthesis that azoles suppresses endogenous gibberellins is imitated, and then suppresses the active list of grease abatement gene in embryo and is reached, the decomposition of grease is hindered,
So as to improve seed oil content；In addition, abscisic acid has antagonism with gibberellin, developmental seed can be enable to obtain dormancy
The expression of gene is cut down in power, same oil-control.
On the other hand, the grain yield of rape is by every plant of pod number, Grain number per pod and grain three factors compositions, borax such as again
Ovule survival rate (improving Grain number per pod) can be promoted, additionally it is possible to reduce fallen flowers rate and colored and unreal rate, it is thus possible to improve
Unit area grain yield.
In view of " oil production=oil content × grain yield ", of the invention from raising seed oil content and two sides of grain yield
Face is started with, and adjusts the component and valid density of foliar fertilizer, reaches the purpose of the increase total oil production of unit area.
A agent and B agent spray that opportunity is very crucial in the present invention, and silique shapes rapidly after initial bloom stage, are oil synthesis with disappearing
Subtract when both compare vigorous, A agent is used before silique shaping, the borax of contained high concentration is conducive to improving result
Rate, the mechanism of action of borax：Protection indoleacetic acid oxidase system is participated in, promotes auxin operating；Borax can cause nectar sugar
Amount increases, thus to insect more attractive, is conducive to the development of flower pesticide and pollen, stimulates the sprouting of pollen, promotes pollen tube
Elongation, and then raising individual plant pod quantity and seeds per pod is played, so as to increase a single plant's output.
The paclobutrazol of higher concentration is the synthesis for suppressing gibberellin in A agent, and compacting grease is decomposed, and promotes oil and fat accumulation,
Other paclobutrazol can moderately reduce the height of plant, resistant to lodging, raising rapeseed plants survival rate.
This stage, using the effect of the abscisic acid of low concentration, mainly causes the antagonism with gibberellin, rather than be
Seed is allowed to obtain dormancy.
The initial bloom stage definition：About 1/4 rape main inflorescence is bloomed.If spraying A agent after initial bloom stage, raising is not just had
Fruiting rate, the effect for reducing colored and unreal increase grain yield.
B agent is applied at the whole florescence, this stage pod has been formed, it is not necessary to which borax is improved in fruiting rate, therefore B agent
Not borax；The concentration of paclobutrazol halves, and the acid concentration that comes off is doubled, primarily to promoting to be stopped developmental embryo
Sleep, dormancy is the stage that oil and fat accumulation was decomposed, promoted in oil-control.
The whole florescence definition：About 3/4 rape flower uses up.If spraying B agent too early, (the optimal spraying time of B agent is whole flower
10-20 days afterwards), just do not have the effect for obtaining dormant trait.
Therefore, present invention also offers a kind of method for improving rape seed oil content, the first initial bloom stage in rape, to
A agent described in rape foliage-spray, the 10-20 days after then starting at the rape whole florescence, to the B described in rape foliage-spray
Agent.
The present invention continues to optimize rape and increases oil foliar fertilizer with when spraying method in practice, preferably, the A agent
The concentration of middle paclobutrazol is 0.3~0.7g/L, and the concentration of abscisic acid is 0.5~1mg/L, and the concentration of boric acid is 15~25g/L.
More preferably, the concentration of paclobutrazol is 0.5g/L in the A agent, the concentration of abscisic acid is 1mg/L, the concentration of boric acid
For 20g/L.
Preferably, the concentration of paclobutrazol is 0.2~0.3g/L in the B agent, the concentration of abscisic acid is 3~7mg/L.
More preferably, the concentration of paclobutrazol is 0.25g/L in the B agent, the concentration of abscisic acid is 5mg/L.
Preferably, the A agent and B agent are sprayed once.
Preferably, the sprayed dose of the A agent and B agent is 30~100 liters every mu.More preferably, every mu sprays 50 liters
Medicament.
Preferably, spraying B agent within the 15th day after starting at the rape whole florescence.
The beneficial effect that the present invention possesses：
The present invention is directed to the different characteristics of rape seed growth and development process, effective in initial bloom stage and whole Spraying in the flowering stage respectively
The different foliar fertilizer of component.Paclobutrazol therein, abscisic acid can effectively oil-control abatement gene active list reach, so as to hinder
The decomposition of seed development stage grease so that " synthesis-abatement " this dynamic equilibrium is towards being conducive to the direction of oil and fat accumulation to incline
Tiltedly, so as to improve seed oil content；Borax composition can not only promote the ovule survival rate of every pod, and can reduce fallen flowers rate
With colored and unreal rate, it is thus possible to improve unit area grain yield.Based on oil production=oil content × grain yield, the present invention
Method be conducive to increase unit area total oil production.
Brief description of the drawings
Fig. 1 is to carry out multifactor multilevel random many fraction of the year field experiment knots of district's groups in Hangzhou, Jinhua two places in embodiment 1
Really, * asterisks represent the marked difference in biometric meaning.
Embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
2 years 2 points of precious spraying effect randomized block experiments of increasing oil
Respectively at 2013-2014, multifactor multilevel random district's groups many fraction of the year are carried out in Hangzhou, Zhejiang province, Jinhua two places
Field experiment.
Empirical factor：1st, medicament kind (P) is sprayed, respectively increases oily treasured, gibberellin, control (clear water)；
2nd, genotype (G), respectively Zhejiang oil 50 (ZY), 9 2-13-58 systems (JR)；
3rd, place (L), respectively Hangzhou (HZ), Jinhua (JH) are sprayed.
4th, scheme is sprayed：
Increase the precious formula of oil：The main component of A agent includes (1) multiple-effect azole material；(2) abscisic acid；(3) composition such as borax.
Female 5 liters of agent " increasing oil precious " (A agent), containing 25 grams of paclobutrazol, 50 milligrams of abscisic acid, 1 kilogram of borax.
Female 0.5 liter of agent " increasing oil precious " (B agent), containing 12.5 grams of paclobutrazol, 250 milligrams of abscisic acid.
The opportunity sprayed and dosage：" increasing oil precious " (A agent) (initial bloom stage is defined in rape initial bloom stage：About 1/4 rape main inflorescence
Bloom) 10 times of sprays of dilution, 1 mu of Rice-rape fields are (i.e.：Every mu sprays 50 liters)；" increasing oil precious " (B agent) 10 days (whole florescences after rape is spent eventually
Definition：About 3/4 rape flower uses up) 100 times of sprays of dilution, 1 mu of Rice-rape fields are (i.e.：Every mu sprays 50 liters).
The concentration of Gibberellins solution is 150 μm of ol L-1。
Spray opportunity and the dosage of gibberellin and clear water increase together oil treasured.
As a result compare as shown in figure 1, spraying and increasing oily treasured, gibberellin, the per unit oil production of control, increase oil it is precious and
The increasing oil of gibberellin is respectively 7.37% and 6.01% with subtracting oil level, and the level of signifiance is reached in statistical significance；
In most cases, spraying increasing oil treasured has different degrees of oil increasing effect, the most it is evident that Hangzhoupro in 2014
State pilot, the oily amplitude of increasing that Zhejiang oil 50 reaches 18% is sprayed with oily treasured is increased.
Embodiment 2
Scheme is sprayed 2015 in Huzhou progress large area experiment using the oily treasured of increasing in embodiment 1, as a result such as the institute of table 1
Show.
Table 1 increases oily precious Huzhou large area pilot spraying test
Remarks：Average per unit area yield has been converted into standard moisture 8%.
The precious rape spraying test result of oil was increased according to Huzhou in 2015,12 pieces 1-1.5 mus spray the precious independence of increasing oil altogether
Plot, sprays and increases 6 pieces of precious plot of oil, and average every mu of volume increase seed adds as 8.38% and 7.21% respectively.
Claims (9)
1. a kind of rape increases oil foliar fertilizer, it is characterised in that including A agent and B agent,
The active ingredient of the A agent is paclobutrazol, abscisic acid and boric acid, and the wherein concentration of paclobutrazol is 0.1~1.0g/L, is come off
The concentration of acid is 0.01~1mg/L, and the concentration of boric acid is 10~30g/L；
The active ingredient of the B agent is paclobutrazol and abscisic acid, and the wherein concentration of paclobutrazol is 0.05~0.5g/L, abscisic acid
Concentration is 0.5~10mg/L.
2. rape as claimed in claim 1 increases oil foliar fertilizer, it is characterised in that the concentration of paclobutrazol is 0.3 in the A agent
~0.7g/L, the concentration of abscisic acid is 0.5~1mg/L, and the concentration of boric acid is 15~25g/L.
3. rape as claimed in claim 2 increases oil foliar fertilizer, it is characterised in that the concentration of paclobutrazol is in the A agent
0.5g/L, the concentration of abscisic acid is 1mg/L, and the concentration of boric acid is 20g/L.
4. the rape as described in claim any one of 1-3 increases oil foliar fertilizer, it is characterised in that paclobutrazol in the B agent
Concentration is 0.2~0.3g/L, and the concentration of abscisic acid is 3~7mg/L.
5. rape as claimed in claim 4 increases oil foliar fertilizer, it is characterised in that the concentration of paclobutrazol is in the B agent
0.25g/L, the concentration of abscisic acid is 5mg/L.
6. a kind of method for improving rape seed oil content, it is characterised in that first in the initial bloom stage of rape, sprays to rape blade face
The A agent described in claim any one of 1-5 is applied, the 10th~20 day after then starting at the rape whole florescence, to rape blade face
Spray the B agent described in claim any one of 1-5.
7. method as claimed in claim 6, it is characterised in that the A agent and B agent are sprayed once.
8. method as claimed in claim 6, it is characterised in that the sprayed dose of the A agent and B agent is 30~100 liters every mu.
9. method as claimed in claim 6, it is characterised in that spray B agent on the 15th day after starting at the rape whole florescence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710352529.3A CN107253871B (en) | 2017-05-18 | 2017-05-18 | Leaf fertilizer for increasing oil content of rape and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710352529.3A CN107253871B (en) | 2017-05-18 | 2017-05-18 | Leaf fertilizer for increasing oil content of rape and application thereof |
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| Publication Number | Publication Date |
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| CN107253871A true CN107253871A (en) | 2017-10-17 |
| CN107253871B CN107253871B (en) | 2020-09-29 |
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| CN201710352529.3A Active CN107253871B (en) | 2017-05-18 | 2017-05-18 | Leaf fertilizer for increasing oil content of rape and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112806221A (en) * | 2021-01-05 | 2021-05-18 | 湖南农业大学 | Method for regulating and controlling oil content of cabbage type rape |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636397A (en) * | 2013-12-20 | 2014-03-19 | 中国农业科学院油料作物研究所 | Method for promoting high production and early-maturing of rapes |
| CN104355896A (en) * | 2014-11-21 | 2015-02-18 | 广东省林业科学研究院 | Special foliage fertilizer for common camellia oleifera |
-
2017
- 2017-05-18 CN CN201710352529.3A patent/CN107253871B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636397A (en) * | 2013-12-20 | 2014-03-19 | 中国农业科学院油料作物研究所 | Method for promoting high production and early-maturing of rapes |
| CN104355896A (en) * | 2014-11-21 | 2015-02-18 | 广东省林业科学研究院 | Special foliage fertilizer for common camellia oleifera |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112806221A (en) * | 2021-01-05 | 2021-05-18 | 湖南农业大学 | Method for regulating and controlling oil content of cabbage type rape |
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