CN107244742B - Method for microbial degradation of pollutants in industrial sewage - Google Patents
Method for microbial degradation of pollutants in industrial sewage Download PDFInfo
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- CN107244742B CN107244742B CN201710326579.4A CN201710326579A CN107244742B CN 107244742 B CN107244742 B CN 107244742B CN 201710326579 A CN201710326579 A CN 201710326579A CN 107244742 B CN107244742 B CN 107244742B
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Abstract
The application discloses a method for microbial degradation of pollutants in industrial sewage, which comprises the following steps: (1) taking industrial sewage of a chemical plant, and testing the initial ammonia nitrogen concentration of the industrial sewage; (2) adding the compound microbial agent into industrial sewage, adding a certain amount of carbon source, and then culturing microorganisms; (3) adding diatomite into industrial sewage of a chemical plant, stirring, and performing suction filtration to obtain a supernatant; (4) adding the compound microbial agent and the carbon source into the supernatant again, and culturing; (5) and centrifuging the supernatant to obtain supernatant, namely the repaired industrial sewage. The method takes the screened high-efficiency heterotrophic nitrification-aerobic denitrification strains, namely the composite microbial inoculum of Raoultella lanuginosa and pseudomonas as a bacterial source, is applied to the purification treatment of high-pollution industrial sewage after inoculation and propagation, and mainly reduces the concentration of overproof ammonia nitrogen. The composite microbial inoculum for treating industrial sewage has the advantages of simple and convenient operation, high efficiency, good treatment effect and the like.
Description
Technical Field
The application relates to a method for microbial degradation of pollutants in industrial sewage, belonging to the technical field of environmental microorganisms.
Background
At present, industrial wastewater generated by a plurality of chemical plants in China is rich in substances such as nitrogen, phosphorus, inorganic salt and the like. If the chemical plant secretly discharges untreated industrial wastewater in violation of laws and regulations, the result of eutrophication can be caused after the industrial wastewater enters a public water source. The conventional treatment method in the prior art is to add chemicals into industrial wastewater for neutralization, then discharge the wastewater after reaching the discharge standard.
The microbial remediation technology is a new technology for remedying the water body pollution which is rapidly developed recently. The remediation technology mainly utilizes the absorption, transformation or degradation of the microorganisms to the pollutants in the water body, thereby achieving the purpose of slowing down or finally eliminating the pollution. The heterotopic microorganism remediation process for treating the chemical plant sewage mainly comprises the steps of adding aerobic autotrophic microorganisms to convert organic nitrogen in the industrial sewage into ammonia nitrogen, carrying out nitrification by nitrifying bacteria and denitrification by denitrifying bacteria, and the like to finally degrade amino nitrogen and nitro nitrogen in a water body, releasing nitrogen to escape from the water body, thereby completing the biogeochemical circulation process of nitrogen in the water body and reducing the concentration of the nitrogen in the water body. The novel microorganism repairing process has low operation cost and small investment; the microorganism finally converts the pollutants into nitrogen, and the influence on the surrounding environment is small; the position of the processing object is not changed, and the operation management is simple and convenient; and the treatment effect is good, and the pollutant removal efficiency is high.
Ectopic microorganism repair technology also has disadvantages, such as the electron acceptor is provided by aeration, and the operation cost is increased; when the amount of added nutrient substances is excessive, secondary pollution can be caused; and the microorganism is sensitive to the change of the environmental temperature and the pH value, and the like.
Accordingly, this application is specifically made to overcome the above-mentioned disadvantages of the prior art.
Disclosure of Invention
In view of the above disadvantages, the present application aims to provide a method for microbial degradation of contaminants in industrial wastewater.
In order to achieve the purpose, the following technical scheme is adopted in the application:
a method for the microbial degradation of contaminants in industrial wastewater, the method comprising the steps of:
(1) taking industrial sewage of a chemical plant, and testing the initial ammonia nitrogen concentration of the industrial sewage;
(2) adding the compound microbial agent into the industrial sewage of the chemical plant obtained in the step (1), adding a carbon source, and then culturing;
(3) adding diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, and performing suction filtration to obtain a supernatant;
(4) adding the compound microbial agent and the carbon source into the supernatant obtained in the step (3) again, and culturing;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain the supernatant, namely the repaired industrial sewage.
Preferably, the compound microbial agent is obtained by screening strains in activated sludge to obtain different high-efficiency heterotrophic nitrification-aerobic denitrification strains, inoculating, expanding, culturing and freezing.
Preferably, the compound microbial agent comprises Raoultella and pseudomonas.
Preferably, the adding amount of the compound microbial agent in the step (2) is 0.5-2% of the volume of the treated industrial sewage.
Preferably, the carbon source comprises sodium citrate or glucose; the adding amount of the carbon source is added according to the carbon-nitrogen ratio of 5:1-10: 1.
Preferably, the incubation described in step (2) and step (4) comprises a reaction at 30 ℃ for 24 hours at a speed of 200 rpm.
Preferably, when the compound microbial inoculum is added again in the step (4), the ammonia nitrogen concentration in the industrial sewage is detected firstly, and when the actual ammonia nitrogen concentration in the sewage is reduced to below 10mg/L, the adding amount of the compound microbial inoculum is 0.1 percent of the volume of the treated industrial sewage.
Preferably, the amount of diatomaceous earth added in step (3) is from 15 to 25 g/L.
Preferably, the method specifically comprises:
(1) taking 50mL of industrial sewage of the Shengjie chemical plant, and detecting the initial ammonia nitrogen concentration of the industrial sewage by using a Naeseler reagent spectrophotometry (HJ 535-2009);
(2) screening activated sludge to obtain different mono-denitrification strains, namely Raoultella lanuginosa and pseudomonas, activating and expanding the strains to obtain the microbial agent, then respectively taking 500 mu L of the microbial agent to be inoculated into industrial sewage, then adding 0.1634g of sodium citrate according to the carbon-nitrogen ratio of 8:1, and culturing for 24 hours at the temperature of 30 ℃ and the rotating speed of 200 rpm;
(3) adding 1.023g of diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, carrying out suction filtration to obtain a supernatant, and detecting the ammonia nitrogen content of the supernatant;
(4) adding the compound microbial agent (500 mu L of composite microbial agent of Raoultella and 500 mu L of composite microbial agent of pseudomonas) and sodium citrate according to the carbon-nitrogen ratio of 5:1 into the supernatant obtained in the step (3), and culturing at the temperature of 30 ℃ and the rotating speed of 200rpm for 24 hours;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain a supernatant which is the repaired industrial sewage, and detecting the content of ammonia nitrogen.
Preferably, the method specifically comprises:
(1) taking 50mL of textile mill sewage, and detecting the initial ammonia nitrogen concentration of the textile mill sewage by using a nano reagent spectrophotometry;
(2) after the composite microbial strains of the Raoultella and the pseudomonas are activated and expanded, respectively taking 25 mu L of the composite microbial strains to be inoculated into industrial sewage, then adding 0.0100g of sodium citrate according to the carbon-nitrogen ratio of 5:1, and culturing for 24 hours at the temperature of 30 ℃ and the rotating speed of 200 rpm;
(3) adding 0.614g of diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, carrying out suction filtration to obtain a supernatant, and detecting the ammonia nitrogen content of the supernatant;
(4) adding the compound microbial agent (25 mu L of composite microbial agent of Raoultella and 25 mu L of composite microbial agent of pseudomonas) and sodium citrate according to the carbon-nitrogen ratio of 5:1 into the supernatant obtained in the step (3), and culturing for 24 hours at the rotation speed of 200rpm under the condition of 30 ℃;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain supernatant, namely the repaired textile mill sewage, and then detecting the ammonia nitrogen content.
The beneficial effects that this application can produce include:
(1) the composite microbial agent provided by the application is a high-efficiency heterotrophic nitrification-aerobic denitrification composite microbial agent, so that the composite microbial agent is used for treating overproof ammonia nitrogen in industrial sewage, and has the advantages of high efficiency, good treatment effect and the like.
(2) The method for the microbial degradation of the industrial sewage, provided by the application, is simple in process method, can greatly reduce the interference of the external environment, and has the advantages of high treatment efficiency, good treatment effect and the like.
(3) The method for adding the nutrient substances necessary for the growth of the microorganisms can solve the problem that the microorganisms are not suitable for growth due to high-pollution industrial sewage, and can reduce the adding amount of the microorganisms.
Detailed Description
The present application will be described in detail with reference to examples, but the present application is not limited to these examples.
The raw materials in the examples of the present application were all purchased commercially, unless otherwise specified.
Example 1: microbial degradation process for industrial sewage of Shengjie chemical plant
A method for ectopic microbial remediation of industrial sewage using a complex microbial inoculant for ectopic microbial remediation of industrial sewage, the method comprising the steps of:
(1) taking 50mL of industrial sewage of the Shengjie chemical plant, and detecting the initial ammonia nitrogen concentration of the industrial sewage by using a Naeseler reagent spectrophotometry (HJ 535-2009);
(2) screening the activated sludge to obtain different mono-denitrification strains, namely Raoultella and pseudomonas, activating and expanding the composite microbial strains, then respectively taking 500 mu L of the activated and expanded composite microbial strains to be inoculated into industrial sewage, then adding 0.1634g of sodium citrate according to the carbon-nitrogen ratio of 8:1, and culturing for 24 hours at the temperature of 30 ℃ and the rotating speed of 200 rpm;
(3) adding 1.023g of diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, carrying out suction filtration to obtain a supernatant, and detecting the ammonia nitrogen content of the supernatant;
(4) adding the compound microbial agent (500 mu L of composite microbial agent of Raoultella and 500 mu L of composite microbial agent of pseudomonas) and sodium citrate according to the carbon-nitrogen ratio of 5:1 into the supernatant obtained in the step (3), and culturing at the temperature of 30 ℃ and the rotating speed of 200rpm for 24 hours;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain a supernatant which is the repaired industrial sewage, and detecting the content of ammonia nitrogen.
The following table shows the detection values of the ammonia nitrogen content at each stage in the repairing process.
| Initial ammonia nitrogen content | 90.51mg/L |
| Ammonia nitrogen content after first inoculation of microorganism | 44.10mg/L |
| Ammonia nitrogen content after second inoculation of microbe | 0.89mg/L |
From the table above, it can be seen that after 2% of microorganisms are inoculated for the first time, the ammonia nitrogen content in the sewage is remarkably reduced, and the removal efficiency of the ammonia nitrogen is close to 50%; after the diatomite is added to remove microorganisms, 2% of microorganisms are inoculated for the second time, the ammonia nitrogen content in the sewage is obviously reduced again, and the ammonia nitrogen content is below 1mg/L, so that the standard of national third-level river water is met.
Example 2: microbial degradation process for sewage of certain textile mill
A method for ectopic microbial remediation of textile mill sewage using a complex microbial inoculant for ectopic microbial remediation of industrial sewage, the method comprising the steps of:
(1) taking 50mL of textile factory sewage, and detecting the initial ammonia nitrogen concentration of the textile factory sewage by using a Nashin reagent spectrophotometry (HJ 535-2009);
(2) after the composite microbial strains of the Raoultella and the pseudomonas are activated and expanded, respectively taking 25 mu L of the composite microbial strains to be inoculated into industrial sewage, then adding 0.0100g of sodium citrate according to the carbon-nitrogen ratio of 5:1, and culturing for 24 hours at the temperature of 30 ℃ and the rotating speed of 200 rpm;
(3) adding 0.614g of diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, carrying out suction filtration to obtain a supernatant, and detecting the ammonia nitrogen content of the supernatant;
(4) adding the compound microbial agent (25 mu L of composite microbial agent of Raoultella and 25 mu L of composite microbial agent of pseudomonas) and sodium citrate according to the carbon-nitrogen ratio of 5:1 into the supernatant obtained in the step (3), and culturing for 24 hours at the rotation speed of 200rpm under the condition of 30 ℃;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain supernatant, namely the repaired textile mill sewage, and then detecting the ammonia nitrogen content.
The following table shows the detection values of the ammonia nitrogen content at each stage in the repairing process.
| Initial ammonia nitrogen content | 5.92mg/L |
| Ammonia nitrogen content after first inoculation of microorganism | 1.67mg/L |
| Ammonia nitrogen content after second inoculation of microbe | 0.48mg/L |
As can be seen from the above table, when the ammonia nitrogen concentration in the sewage is below 10mg/L, the microbial inoculation amount can be reduced to 0.1%. After 0.1% of composite microbial inoculum is inoculated for the first time, the content of ammonia nitrogen in the sewage is reduced, and the removal efficiency of the ammonia nitrogen is close to 72%; after the diatomite is added to remove the microorganisms, the ammonia nitrogen content in the sewage is reduced to below 1mg/L again after 0.1 percent of microorganisms are inoculated for the second time.
Although the present application has been described with reference to a few embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the application as defined by the appended claims.
Claims (7)
1. A method for the microbial degradation of contaminants in industrial wastewater, the method comprising the steps of:
(1) taking industrial sewage of a chemical plant, and testing the initial ammonia nitrogen concentration of the industrial sewage;
(2) adding the compound microbial agent into the industrial sewage of the chemical plant obtained in the step (1), adding a carbon source, and then culturing; the compound microbial agent is obtained by screening strains in the activated sludge to obtain different high-efficiency heterotrophic nitrification-aerobic denitrification strains, inoculating, expanding, culturing and freezing; the compound microbial agent comprises Raoultella and pseudomonas; the adding amount of the compound microbial agent is 0.1-2% of the volume of the treated industrial sewage;
(3) adding diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, and performing suction filtration to obtain a supernatant;
(4) adding the compound microbial agent and the carbon source into the supernatant obtained in the step (3) again, and culturing;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain the supernatant, namely the repaired industrial sewage.
2. The method of claim 1, wherein the carbon source comprises sodium citrate or glucose; the adding amount of the carbon source is added according to the carbon-nitrogen ratio of 5:1-10: 1.
3. The method of claim 1, wherein the incubation in steps (2) and (4) comprises a reaction at 200rpm at 30 ℃ for 24 hours.
4. The method according to claim 1, wherein when the compound microbial inoculum is added again in step (4), the ammonia nitrogen concentration in the industrial sewage is detected, and when the actual ammonia nitrogen concentration in the sewage is reduced to below 10mg/L, the adding amount of the compound microbial inoculum is 0.1% of the volume of the treated industrial sewage.
5. The method according to claim 1, wherein the amount of diatomaceous earth added in step (3) is 15 to 25 g/L.
6. The method according to claim 1, characterized in that it comprises in particular:
(1) taking 50mL of industrial sewage of a certain factory, and detecting the initial ammonia nitrogen concentration of the industrial sewage by using a Nashiner reagent spectrophotometry, namely HJ 535-2009;
(2) screening activated sludge to obtain different mono-denitrification strains, namely Raoultella lanuginosa and pseudomonas, activating and expanding the strains to obtain the microbial agent, then respectively taking 500 mu L of the microbial agent to be inoculated into industrial sewage, then adding 0.1634g of sodium citrate according to the carbon-nitrogen ratio of 8:1, and culturing for 24 hours at the temperature of 30 ℃ and the rotating speed of 200 rpm;
(3) adding 1.023g of diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, carrying out suction filtration to obtain a supernatant, and detecting the ammonia nitrogen content of the supernatant;
(4) adding the compound microbial agent into the supernatant obtained in the step (3) again, adding sodium citrate according to the carbon-nitrogen ratio of 5:1, and culturing at the temperature of 30 ℃ and the rotating speed of 200rpm for 24 hours; the compound microbial agent is as follows: 500 mul of composite bacterial preparation of Raoultella and 500 mul of pseudomonad;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain a supernatant which is the repaired industrial sewage, and detecting the content of ammonia nitrogen.
7. The method according to claim 1, characterized in that it comprises in particular:
(1) taking 50mL of textile mill sewage, and detecting the initial ammonia nitrogen concentration of the textile mill sewage by using a nano reagent spectrophotometry;
(2) after the composite microbial strains of the Raoultella and the pseudomonas are activated and expanded, respectively taking 25 mu L of the composite microbial strains to be inoculated into industrial sewage, then adding 0.0100g of sodium citrate according to the carbon-nitrogen ratio of 5:1, and culturing for 24 hours at the temperature of 30 ℃ and the rotating speed of 200 rpm;
(3) adding 0.614g of diatomite into the industrial sewage of the chemical plant obtained in the step (2), stirring, carrying out suction filtration to obtain a supernatant, and detecting the ammonia nitrogen content of the supernatant;
(4) adding the composite microbial agent into the supernatant obtained in the step (3) again, adding sodium citrate according to the carbon-nitrogen ratio of 5:1, and culturing at 30 ℃ and at the rotating speed of 200rpm for 24 hours again; the compound microbial agent is as follows: 25 mu L of composite microbial inoculum of Raoultella and 25 mu L of pseudomonas;
(5) and (4) centrifuging the supernatant obtained in the step (4) to obtain supernatant, namely the repaired textile mill sewage, and then detecting the ammonia nitrogen content.
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| CN109055252A (en) * | 2017-11-13 | 2018-12-21 | 鼎正新兴生物技术（天津）有限公司 | Heterotrophic nitrification-aerobic denitrification composite microbial preparation and preparation method thereof |
| CN108753762A (en) * | 2018-06-07 | 2018-11-06 | 陈帅 | A method of processing waste water |
| CN111056645A (en) * | 2019-08-07 | 2020-04-24 | 上田环境修复有限公司 | Microbial treatment method applied to river cross section restoration |
| CN110846246A (en) * | 2019-11-06 | 2020-02-28 | 广东省生物资源应用研究所 | Preliminary screening method for bacteria with ammonia nitrogen degradation function |
| CN110835204A (en) * | 2019-11-07 | 2020-02-25 | 上田环境修复有限公司 | Method for degrading ammonia nitrogen in wastewater |
| CN113604407B (en) * | 2021-09-03 | 2024-02-06 | 中冶华天南京工程技术有限公司 | Composite microbial algicidal fungicide and preparation method and application thereof |
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