CN107232210A - Application of the thiadiazole compound on paddy bacterial rice shoot damping-off control medicament is prepared - Google Patents
Application of the thiadiazole compound on paddy bacterial rice shoot damping-off control medicament is prepared Download PDFInfo
- Publication number
- CN107232210A CN107232210A CN201710317272.8A CN201710317272A CN107232210A CN 107232210 A CN107232210 A CN 107232210A CN 201710317272 A CN201710317272 A CN 201710317272A CN 107232210 A CN107232210 A CN 107232210A
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- CN
- China
- Prior art keywords
- thiadiazoles
- sulfydryl
- rice
- plantarii
- amino
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Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 139
- 235000009566 rice Nutrition 0.000 title claims abstract description 139
- -1 thiadiazole compound Chemical class 0.000 title claims abstract description 45
- 241000918585 Pythium aphanidermatum Species 0.000 title claims abstract description 29
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 17
- 239000003814 drug Substances 0.000 title claims abstract description 8
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 138
- 241000134107 Burkholderia plantarii Species 0.000 claims abstract description 86
- 239000000575 pesticide Substances 0.000 claims abstract description 25
- BIGYLAKFCGVRAN-UHFFFAOYSA-N 1,3,4-thiadiazolidine-2,5-dithione Chemical class S=C1NNC(=S)S1 BIGYLAKFCGVRAN-UHFFFAOYSA-N 0.000 claims description 42
- LVJOFJVCBFMGPW-UHFFFAOYSA-N SC1=C(C(=O)O)C=C(C=C1C(=O)O)C Chemical class SC1=C(C(=O)O)C=C(C=C1C(=O)O)C LVJOFJVCBFMGPW-UHFFFAOYSA-N 0.000 claims description 38
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 claims description 31
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 28
- 239000002689 soil Substances 0.000 claims description 19
- 239000003337 fertilizer Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 9
- 239000004480 active ingredient Substances 0.000 claims description 7
- 230000034303 cell budding Effects 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 150000004869 1,3,4-thiadiazoles Chemical class 0.000 claims 1
- 239000003053 toxin Substances 0.000 abstract description 23
- 231100000765 toxin Toxicity 0.000 abstract description 23
- MDYOLVRUBBJPFM-UHFFFAOYSA-N tropolone Chemical compound OC1=CC=CC=CC1=O MDYOLVRUBBJPFM-UHFFFAOYSA-N 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 66
- 238000012545 processing Methods 0.000 description 41
- GDGIVSREGUOIJZ-UHFFFAOYSA-N 5-amino-3h-1,3,4-thiadiazole-2-thione Chemical class NC1=NN=C(S)S1 GDGIVSREGUOIJZ-UHFFFAOYSA-N 0.000 description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 31
- 230000003449 preventive effect Effects 0.000 description 27
- 238000011081 inoculation Methods 0.000 description 24
- 241000196324 Embryophyta Species 0.000 description 19
- 201000010099 disease Diseases 0.000 description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 230000012010 growth Effects 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
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- 238000007619 statistical method Methods 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 150000004867 thiadiazoles Chemical class 0.000 description 6
- AIDLAEPHWROGFI-UHFFFAOYSA-N 2-methylbenzene-1,3-dicarboxylic acid Chemical class CC1=C(C(O)=O)C=CC=C1C(O)=O AIDLAEPHWROGFI-UHFFFAOYSA-N 0.000 description 5
- 239000005794 Hymexazol Substances 0.000 description 5
- 239000000654 additive Substances 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 description 5
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- 229930192474 thiophene Natural products 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
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- 208000035143 Bacterial infection Diseases 0.000 description 2
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- WTKZEGDFNFYCGP-UHFFFAOYSA-N Pyrazole Chemical compound C=1C=NNC=1 WTKZEGDFNFYCGP-UHFFFAOYSA-N 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
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- 150000003851 azoles Chemical class 0.000 description 2
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- 229910052742 iron Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- FTQWRYSLUYAIRQ-UHFFFAOYSA-N n-[(octadecanoylamino)methyl]octadecanamide Chemical class CCCCCCCCCCCCCCCCCC(=O)NCNC(=O)CCCCCCCCCCCCCCCCC FTQWRYSLUYAIRQ-UHFFFAOYSA-N 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
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- 238000004659 sterilization and disinfection Methods 0.000 description 2
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- 239000000304 virulence factor Substances 0.000 description 2
- 230000007923 virulence factor Effects 0.000 description 2
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- FPVUWZFFEGYCGB-UHFFFAOYSA-N 5-methyl-3h-1,3,4-thiadiazole-2-thione Chemical class CC1=NN=C(S)S1 FPVUWZFFEGYCGB-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical class N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 241001453380 Burkholderia Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002148 Gellan gum Polymers 0.000 description 1
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 1
- 208000025309 Hair disease Diseases 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical compound C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
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- 239000003905 agrochemical Substances 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 229940099112 cornstarch Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000034653 disorder of pilosebaceous unit Diseases 0.000 description 1
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- 229910052564 epsomite Inorganic materials 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229940084434 fungoid Drugs 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000000216 gellan gum Substances 0.000 description 1
- 235000010492 gellan gum Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- ZLTPDFXIESTBQG-UHFFFAOYSA-N isothiazole Chemical compound C=1C=NSC=1 ZLTPDFXIESTBQG-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- HCWCAKKEBCNQJP-UHFFFAOYSA-N magnesium orthosilicate Chemical compound [Mg+2].[Mg+2].[O-][Si]([O-])([O-])[O-] HCWCAKKEBCNQJP-UHFFFAOYSA-N 0.000 description 1
- 239000000391 magnesium silicate Substances 0.000 description 1
- 229910052919 magnesium silicate Inorganic materials 0.000 description 1
- 235000019792 magnesium silicate Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- PXQLVRUNWNTZOS-UHFFFAOYSA-N sulfanyl Chemical class [SH] PXQLVRUNWNTZOS-UHFFFAOYSA-N 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 150000003557 thiazoles Chemical class 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Soil Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of the thiadiazole compound on paddy bacterial rice shoot damping-off control medicament is prepared, the preparation that the present invention is prepared by principle active component of thiadiazole compound, the level that B.plantarii produces toxin tropolone can effectively be suppressed, and then prevent and treat paddy bacterial rice shoot damping-off.Simple and easy to apply using pesticide control of the present invention preventing and treating paddy bacterial rice shoot damping-off, cost is low, and prevention effect is good.
Description
Technical field
The present invention relates to pesticide science field, more particularly to thiadiazole compound stands withered preparing paddy bacterial rice shoot
Application on sick pesticide control.
Background technology
Plant burkholderia (Burkholderia plantarii) causes the bacillary damping-off of rice seedling
One of important pathogen, its infectivity, reproductive capacity and adaptability are very strong, seriously threaten Rice Production.In nature,
B.plantarii is often distributed in seedbed soil, water in field, vegetable seeds and plant and animal residues, into rice seedling bed after in suitable condition
Lower completion First aggression, and Infection cycie again is entered by seed and soil-borne.The rice shoot infected shows as stunting in early stage,
Chlorosis and root growth and development obstacle, late blight are dead.
Pathological research finds that B.plantarii secretes small point that one kind is referred to as tropolone (tropolone)
Sub- virulence factor, it forms insoluble chelate with reference to iron ion, induces plant shoots root system too little iron, causes growth of seedling
Slow and final withered death (such as Fig. 1).The disease was once popular and multiple in southern US and south east asia in the Japanese whole nation
Outburst, catastrophic loss is brought to Rice Production.Last decade, has diffused to southern US, Southeast Asia, South Korea and China.
The disease was found report in 2014 in China first, had been sent out in Chinese multiple paddy rice main producing regions at present
It is raw, and with diffusion tendency.Because the seedling season of rice in China main producing region is generally hot and humid weather conditions, this is
B.plantarii growth, breeding and diffusion creates favourable condition.Especially the aggravation of Global Greenhouse Effect in recent years and
The popularization of greenhouse seedling-cultivation technique, also aggravates eruption and prevalence of the disease in China.
At present, the agricultural chemicals of in the market preventing and treating bacterial disease mainly has Inorganic Copper class, organic copper class and thiazoles, for example：
Application publication number discloses a kind of slow release sterilization (worm) group for CN106508917A application for a patent for invention document
Compound and its application, said composition are that allyl isothiazole and hymexazol are constituted, or allyl isothiazole, hymexazol and bisamide class are killed
Worm agent is constituted；Count by weight, the weight ratio between allyl isothiazole and activearm hymexazol is 1:50 to 50:1；Allyl is different
The ratio of weight and number of thiazole and hymexazol weight sum and bisamide insecticides is 20:1 to 200:1.Alkene in invention combination
Third isothiazole and hymexazol mixture, expand control range of the medicament to crop disease.Such as the combination is applied in the seedling cultivation of rice phase
Thing, can prevent and treat simultaneously rice seedling blight (paddy rice fungoid damping-off caused by Rhizoctonia and Fusarium), bacterial wilt and
Rice blast.
But, effective pesticide control specifically designed for the paddy bacterial disease is there is no so far.Therefore, it is necessary to thin to this
Bacterium property rice shoot damping-off makes further research, to obtain special effectively pesticide control.
The content of the invention
The present invention is intended to provide the bacillary damping-off of rice seedling caused by thiadiazole compound preventing and treating B.plantarii
New application, and in particular to application of the thiadiazole compound on paddy bacterial rice shoot damping-off control medicament is prepared.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
The invention provides application of the thiadiazole compound on paddy bacterial rice shoot damping-off control medicament is prepared.
Preferably, the thiadiazole compound is 2- amino -5- sulfydryls -1,3,4- thiadiazoles, 2,5- dimercapto -1,
3,4- thiadiazoles or 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles.
The structural formula of above-claimed cpd, is shown below：
2- amino -5- sulfydryl -1,3,4- thiadiazoles
2,5- dimercapto -1,3,4- thiadiazoles
2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles
Wherein, the thiadiazole compound being related in the present invention is including the use of the natural extraction containing thiadiazole compound
Thing, commercial synthesis thing, the extract of the accessory substance of industrial treatment, but be not limited thereto.Except above-mentioned three kinds of thiadiazole
Compound, can also use its derivative, and the derivative refers to the salt, ester, acyl received in thiadiazole compound pesticidal preparations
The derivative of ammonia, glycocide, but be not limited thereto.
Further, the paddy bacterial rice shoot damping-off is caused by burkholderia B.plantarii.
The invention provides a kind of pesticide control, its active ingredient is thiadiazole compound.Above-mentioned pesticide control, both may be used
To be single dose, i.e., pure thiadiazole compound solution (2- amino -5- sulfydryls -1,3,4- thiadiazoles or 2,5- dimercapto -1,3,
4- thiadiazoles or 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles) or compounding, i.e.,：Include two of which or two or more
Combination.
Preferably, the thiadiazole compound is 2- amino -5- sulfydryls -1,3,4- thiadiazoles, 2,5- dimercapto -1,
At least one of 3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles.
Preferably, the concentration of the thiadiazole compound is 0.005~5mM.
Further preferably, the active ingredient be 2- amino -5- sulfydryls -1,3,4- thiadiazoles and 2,5- dimercapto -1,
3,4- thiadiazoles, its concentration is respectively 0.005~5mM and 0.005~5mM；Or, the active ingredient is 2- amino -5- mercaptos
Base -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles, its concentration be respectively 0.005~5mM and 0.005~
5mM。
Present invention also offers a kind of pesticide control, it is calculated in mass percent, including following components：
Thiadiazole compound 0.006~0.142%；
Dimethyl sulfoxide (DMSO) 0.010%；
Ultra-pure water 99.848~99.9839%.
Preferably, in above-mentioned pesticide control, the concentration of thiadiazole compound is 0.005~5mM；Thiadiazole chemical combination
Thing is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4-
At least one of thiadiazoles.
Further preferably, the thiadiazole compound is 2- amino -5- sulfydryls -1,3,4- thiadiazoles and the mercapto of 2,5- bis-
Base -1,3,4- thiadiazoles, its concentration is respectively 0.005-5mM and 0.005-5mM；Or be 2- amino -5- sulfydryls -1,3,4-
Thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles, its concentration is respectively 0.005-5mM and 0.005-5mM；Or be 2,
5- dimercaptos -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles, its concentration be respectively 0.005-5mM and
0.005-5mM。
In addition, thiadiazole compound can be any suitable form, such as solid, semi-solid, liquid or aerosol shape
Prepared by formula, acceptable carriers include, for example, water, glucose, lactose, Arabic gum, gelatin, mannitol, starch, three
Magnesium silicate, talcum, cornstarch, keratin, colloidal silica, farina, sandy soil, nano titanium oxide, and be adapted to
Other carriers used in the preparation for preparing solid, semisolid, liquid or aerosol form；Combinations thereof can contain in addition
Stabilizer, thickener, and/or colouring agent and spices, but be not limited thereto.
Show through isolated experiment, thiadiazole compound of the present invention produces toxin to B.plantarii
Tropolone level, which has, significantly inhibits effect.When thiadiazole compound concentration be 0.005mM when, toxin relative to
Control group shows reduction trend；When concentration for the treatment of rises to 0.05mM, toxin produces level and reduces 30%；When concentration for the treatment of liter
During to 5mM, toxin produces level and reduces 90%.In experiment made on the living, it has been found that thiadiazole compound can effectively suppress
The illness for the rice seedlings that B.plantarii infects, after thiadiazole compound is handled with different insecticide-applying way, reaches ideal
Prevention effect, wherein combination dispenser also act as synergistic effect.Meanwhile, thiadiazole compound can also be to the rice seedlings do not caught an illness
Growth-promoting effect can also be played.Therefore, thiadiazole compound can be for preparing paddy bacterial rice shoot damping-off control
Medicament.
Present invention also offers a kind of method for preventing and treating paddy bacterial rice shoot damping-off, rice paddy seed is soaked in described
In pesticide control；Or, pesticide control drop is imposed on into rice seedling bed；Or, the pesticide control is mixed into sandy soil and spread fertilizer over the fields in rice seedling bed；
Or to spraying the pesticide control in budding rice seedling.
Specifically, excuted a law for drop, prior to seeding, by the pesticide control, uniformly drop is applied to seedbed rice seedling bed water layer, keeps
Water layer more than 7 days.For mixing sandy soil method, before sowing, the pesticide control and sandy soil are mixed thoroughly, rice seedling bed is uniformly spread fertilizer over the fields, water is kept
Layer more than 7 days.
Compared with prior art, the invention has the advantages that：
(1) it is bacillary vertical withered the invention provides rice seedling caused by thiadiazole compound preventing and treating B.plantarii
The new application of disease.
(2) pesticide control and the method for preventing and treating paddy bacterial rice shoot damping-off that the present invention is provided can effectively suppress
B. plantarii produces toxin tropolone level, and then prevents and treats paddy bacterial rice shoot damping-off.
Brief description of the drawings
Virulence factor and the pathogenesis to rice seedling that Fig. 1 is B.plantarii.
Fig. 2 is the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of various concentrations in embodiment 1 to B.plantarii toxin point
The influence secreted.
Fig. 3 is various concentrations 2,5- dimercapto -1,3,4- thiadiazoles in embodiment 1 to B.plantarii toxin secretions
Influence.
Fig. 4 is various concentrations 2- sulfydryl -5- methyl isophthalic acids in embodiment 1, and 3,4- thiadiazoles is to B.plantarii toxin secretions
Influence.
Fig. 5 adds 2- amino -5- sulfydryl -1,3,4- thiadiazoles to bacillary damping-off for MS culture mediums in embodiment 2
Typical preventive effect diagram；
Wherein,
A is to plant the water in without 2- amino -5- sulfydryl -1,3,4- thiadiazoles culture mediums after being inoculated with B.plantarii
Rice seedling；B is young to plant the paddy rice in the -5- sulfydryl -1,3,4- thiadiazoles culture mediums of amino containing 2- after inoculation B.plantarii
Seedling；C is young not to be inoculated with B.plantarii and planting the paddy rice in the -5- sulfydryl -1,3,4- thiadiazoles culture mediums of amino containing 2-
Seedling；D is is not inoculated with B.plantarii and plants the paddy rice in without 2- amino -5- sulfydryl -1,3,4- thiadiazoles culture mediums
Seedling.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.It should be understood that following examples are merely to illustrate this
Invention is not for limitation the scope of the present invention.
The form annotation being related in the following example is as follows：(embodiment 2-7 and form annotation described in thiadiazole
Polymer solution or 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiadiazoles
Solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution (0.005mM-5mM) are referred both to through water and 1% 2
Mixed solution after methyl sulfoxide dissolving.)
Table 1-3 is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles, 2- in embodiment 2
Sulfydryl -5- methyl isophthalic acids, preventive effect of the 3,4- thiadiazoles in matrix additive process
Wherein, A is the rice seedlings without any processing；B is to be planted after being inoculated with B.plantarii in without thiadiazole
Rice seedling in compound culture medium；C, D, E are to be planted after being inoculated with B.plantarii in containing thiadiazole compound
Rice seedling in (0.005mM, 0.05mM, 5mM) culture medium；F is the paddy rice using only 0.05mM thiadiazole compounds
Seedling.
Table 4-6 is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles, 2- in embodiment 3
Sulfydryl -5- methyl isophthalic acids, preventive effect of the 3,4- thiadiazoles in seed treatment；
Wherein, A is the rice seedlings without any processing；B is molten with 0.01% dimethyl sulfoxide (DMSO) after inoculation B.plantarii
Liquid carries out the rice seedling of seed treatment；C, D, E are to use thiadiazole compound solution after being inoculated with B.plantarii
(0.005mM, 0.05mM, 5mM) carries out the rice seedling of seed treatment；F is the water using only 0.05mM thiadiazole compounds
Rice seedling.
Table 7-9 is 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles, 2- in embodiment 4
Sulfydryl -5- methyl isophthalic acids, preventive effect of the 3,4- thiadiazoles in spraying method；
Wherein, A is the rice seedlings without any processing；B is molten with 0.01% dimethyl sulfoxide (DMSO) after inoculation B.plantarii
Liquid spray the rice seedling of processing；C, D, E are to use thiadiazole compound solution after being inoculated with B.plantarii
(0.005mM, 0.05mM, 5mM) spray the rice seedling of processing；F is the water using only 0.05mM thiadiazole compounds
Rice seedling.
Table 10-12 be embodiment 5 in 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles,
2- sulfydryl -5- methyl isophthalic acids, preventive effect of the 3,4- thiadiazoles in being excuted a law to water droplet
Wherein, A is the rice seedlings without any processing；B is plants in using 0.01% dimethyl after inoculation B.plantarii
Sulfoxide solution water droplet is applied the rice seedlings in processing rice seedling bed；C, D, E for after inoculation B.plantarii with planting in thiadiazole
Compound solution (0.005mM, 0.05mM, 5mM) applies the rice seedlings in processing rice seedling bed to water droplet；F is to plant in using only 0.05mM
Thiadiazole compound applies the rice seedlings in processing rice seedling bed to water droplet.
Table 13-15 be embodiment 6 in 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles,
2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles mixes the preventive effect in husky broadcast application
Wherein, A is the rice seedlings without any processing；B is plants in using 0.01% dimethyl after inoculation B.plantarii
Sulfoxide solution mixes the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed；C, D, E is plant in using thiadiazole after inoculation B.plantarii
Polymer solution (0.005mM, 0.05mM, 5mM) carries out mixing the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed；F for plant in using only
0.05mM thiadiazole compounds mix the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed.
Table 16-18 be embodiment 7 in 2- amino -5- sulfydryl -1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles,
2- sulfydryl -5- methyl isophthalic acids, preventive effect of the 3,4- thiadiazoles compounded combination liquid in husky broadcast application is mixed
Wherein, A is the rice seedlings without any processing；B is plants in using 0.01% dimethyl after inoculation B.plantarii
Sulfoxide solution mixes the rice seedlings that sandy soil spread fertilizer over the fields processing rice seedling bed；C, D, E is inoculation B.plantarii and plant in combination solution
(0.005mM, 0.05mM, 5mM) treats the rice seedlings in rice seedling bed；F is plants in mixing sandy soil using only combination solution (0.05mM)
Spread fertilizer over the fields the rice seedlings in processing rice seedling bed.
Inhibitory action of the thiadiazole compound of embodiment 1 to B.plantarii toxin secretions
1 B.plantarii culture
Plant burkholderia B.plantarii strain isolations are from Zhuji, zhejiang rice field, and daily culture is in 25 DEG C of constant temperature
In incubator, the culture medium of selection is nutrient broth agar culture medium.
2 B.plantarii inoculation
With the vertical picking B.plantarii of transfer needle single bacterium colony, it is placed in 50ml nutrient broth mediums and carries out
Preculture, after cultivating 24 hours, draws abandoning supernatant after 10ml bacterium solutions, 5000rpm centrifugations 5min, adds equivalent clear water clear
Wash, after concussion is mixed, then with 5000rpm centrifugation 5min, equivalent clear water is added again and mixing is shaken, the bacterium after mixing is drawn
The μ L of liquid 100 add into 60ml MA fluid nutrient mediums culture, and the formula rate of MA culture mediums is inorganic salt solution (NH4H2PO4
0.0.01%, KCl 0.02%, MgSO4·7H2O 0.02%) 200X, glucose 10g, water 1L.
The influence for the toxin tropolone that 3 2- amino -5- sulfydryl -1,3,4- thiadiazoles are secreted to B.plantarii
2- amino -5- sulfydryl -1,3,4- thiadiazoles is added in inoculation B.plantarii MA culture mediums respectively
0.005mM、0.05 mM、0.5mM、5mM；And the control that leaves some space, each experiment group is repeated 3 times, after the completion of will contain
The culture medium of B.plantarii and 2- amino -5- sulfydryl -1,3,4- thiadiazoles is placed on 25 DEG C of isothermal vibration cultures.Cultivate rank
Section, every 12h draw 10ml bacterium solutions into 50ml centrifuge tubes, 5000rpm centrifugation 5min after, by supernatant be transferred to it is new from
In heart pipe, its pH is adjusted to 3,10ml acetonitriles are then added, add 1gNaCl, after fully shaking is mixed, solution is divided into two
Layer, Aspirate supernatant 2ml is crossed after 0.22 μm of organic film, then is settled in sample injection bottle, and laggard GC-MS/MS analysis detections.
As shown in Fig. 2 when 2- amino -5- sulfydryls -1,3, the concentration of 4- thiadiazoles is 0.005mM-5mM, B.plantarii
Toxin tropolone level is produced, in gradient depression effect.When the concentration of 2- amino -5- sulfydryl -1,3,4- thiadiazoles is
During 0.05mM, toxin produces level and reduces 30%；When 2- amino -5- sulfydryls -1,3, when the concentration of 4- thiadiazoles is 5mM, toxin
The inhibiting rate of generation is up to 88%.(Fig. 2).This shows that 2- amino -5- sulfydryls -1,3,4- thiadiazoles can effectively suppress
The generation of B.plantarii toxin.
The influence for the toxin tropolone that 4 2,5- dimercapto -1,3,4- thiadiazoles are secreted to B.plantarii
Respectively 2,5- dimercapto-1,3,4-thiadiazoles are added in inoculation B.plantarii MA culture mediums, 0.005mM,
0.05 mM, 0.5mM, 5mM, and the control that leaves some space, each experiment group are repeated 3 times, after the completion of will contain B.plantarii
25 DEG C of isothermal vibration cultures are placed on the culture medium of 2,5- dimercapto -1,3,4- thiadiazoles.In culture to 96h, 10ml bacterium are drawn
Liquid, GC-MS/MS analysis detection toxin situations.
As shown in figure 3, when the concentration of 2,5- dimercapto-1,3,4-thiadiazoles is 0.005mM-5mM, B.plantarii
Toxin is produced and is suppressed, and certain dose-dependent effect is presented, and maximal percentage inhibition is up to 86%.This shows, 2,5- bis- mercaptos
Base -1,3,4- thiadiazoles can effectively suppress the generation of B.plantarii toxin.
5 2- sulfydryl -5- methyl isophthalic acids, the influence for the toxin tropolone that 3,4- thiadiazoles is secreted to B.plantarii
Respectively inoculation B.plantarii MA culture mediums in add 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles,
0.005mM, 0.05 mM, 0.5mM, 5mM, and the control that leaves some space, each experiment group are repeated 3 times, after the completion of will contain
B.plantarii and 2- sulfydryl -5- methyl isophthalic acids, the culture medium of 3,4- thiadiazoles is placed on 25 DEG C of isothermal vibration cultures.In culture extremely
96h, draws 10ml bacterium solutions, GC-MS/MS analysis detection toxin situations.
As shown in figure 4, when 2- sulfydryl -5- methyl isophthalic acids, when the concentration of 3,4- thiadiazoles is 0.005mM-5mM,
B.plantarii toxin is produced and is suppressed, and certain dose-dependent effect is presented, and maximal percentage inhibition is up to 79%.This table
Bright, 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles can effectively suppress the generation of B.plantarii toxin.
The growth substrate additive process of embodiment 2
1 paddy rice vernalization
Sufficient amount of rice paddy seed is chosen, is carried out using 2% sodium hypochlorite after surface sterilization half an hour, after fully cleaning
It is placed in sterilizing glass culture dish, adds a small amount of sterilizing clear water (not have the surface of the seed to be defined), 25 are placed on afterwards
Cultivated in DEG C constant incubator, every other day need to change the clear water that once sterilizes, after 2-3 days, the phenomenon that shows money or valuables one carries unintentionally occurs in seed, now
Vernalization is completed.
2 inoculation B.plantarii
With the vertical picking B.plantarii of transfer needle single bacterium colony, it is placed in 50ml nutrient broth mediums and carries out
Preculture, after cultivating 24 hours, draws abandoning supernatant after 10ml bacterium solutions, 5000rpm centrifugations 5min, adds equivalent clear water clear
Wash, after concussion is mixed, then with 5000rpm centrifugation 5min, equivalent clear water is added again and mixing is shaken.After the completion of vernalization
Rice paddy seed is divided into two batches, is separately added into and prepares B.plantarii bacteria suspensions as inoculation group or isometric sterilizing clear water
As not inoculation group (not have the surface of the seed to be defined), cultivate 24 hours.
Efficiency test of the 3 2- amino -5- sulfydryl -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
The MS plant cultures of certain volume are prepared, 2- amino -5- sulfydryls -1,3,4- thiadiazoles are added respectively
(0.005mM, 0.05mM, 5mM) and without 2- amino -5- sulfydryls -1,3,4- thiadiazoles is dispensed into blake bottle, every bottle
20ml, the gellan gum of every bottle of addition 1.5% becomes solid medium.Then B.plantarii will be inoculated with and be not inoculated with
B.plantarii rice paddy seed, plantation is to containing and not containing 2- amino -5- sulfydryls -1,3 respectively, and the MS of 4- thiadiazoles is trained
Support base, 12 every bottle.After each experiment group is provided with, and blake bottle is put into 25 DEG C of constant incubators, daily illumination
12h dark 12h is cultivated rice seedlings, its upgrowth situation of continuous observation, and determines at the 3rd day the length of rice seedlings root and leaf
Degree, carries out statistical analysis.
As a result find, compared with the rice seedlings for not being inoculated with B.plantarii, the rice seedlings table after inoculation B.plantarii
Reveal obvious illness (yellow and stunt), overground part and root growth are significantly inhibited (Fig. 5 A)；Inoculation
B.plantarii rice seedlings are after addition 2- amino -5- sulfydryls -1,3,4- thiadiazoles, and ongoing disease is relieved (Fig. 5 B), and
The rice growth situation for not being inoculated with B.plantarii is close；Meanwhile, contrast is not inoculated with B.plantarii rice seedlings, adds 2-
Amino -5- sulfydryl -1,3,4- thiadiazoles can also play slight facilitation (Fig. 5 C and Fig. 5 D) to paddy growth.In addition, water
The length statistical analysis of the root and leaf of rice seedling shows (table 1) that 2- amino -5- sulfydryls -1,3,4- thiadiazoles can effectively control
The bacillary damping-off of rice seedling caused by B.plantarii.
Preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table 1. in matrix additive process
| Processing | Stem length (cm) | Root length (cm) |
| A | 4.5 | 1.8 |
| B | 0.8 | 0.2 |
| C | 3.5** | 0.8** |
| D | 4.2** | 1.5** |
| E | 4.8** | 1.9** |
| F | 5.1 | 2 |
**P<0.005
As shown in Table 1, compared with the paddy growth situation without any processing, be only inoculated with the paddy rice after B.plantarii
Growth is substantially suppressed, and using only 2- amino -5- sulfydryls -1,3, the paddy growth of 4- thiadiazoles processing has but been obtained slightly
Promotion (P ﹤ 0.05).In addition, being inoculated with B.plantarii and using 0.005mM-5mM 2- amino -5- sulfydryls -1,3,4- thiophenes
The growth inhibition of the rice seedlings of diazole processing has obtained obvious alleviation (P ﹤ 0.005).By comparing different experiments group paddy rice
The root of seedling and the length (table 1) of leaf, we have also obtained conclusion similar to the above, this explanation 2- amino -5- sulfydryls -1,3,4-
Thiadiazoles can effectively prevent and treat B.plantarii and infect caused rice shoot damping-off after paddy rice.
Efficiency test of the 4 2,5- dimercapto -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
Operating procedure is according to " preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
Experiment " is carried out, and test compound only is changed into 2,5- dimercapto-1,3,4-thiadiazoles.
Preventive effect of the 2,5- dimercapto -1,3,4- thiadiazoles of table 2. in matrix additive process
| Processing | Stem length (cm) | Root length (cm) |
| A | 4.3 | 1.6 |
| B | 0.5 | 0.2 |
| C | 3.7** | 1.0** |
| D | 3.9** | 1.4** |
| E | 4.4** | 1.7** |
| F | 4.5 | 1.5 |
**P<0.005
As shown in Table 2, B.plantarii rice seedlings, 2,5- dimercaptos -1,3 through 0.005mM-5mM, 4- thiophenes are inoculated with
Diazole processing, disease symptom obtains significantly alleviating (P ﹤ 0.005).Illustrate that 2,5- dimercapto -1,3,4- thiadiazoles can effectively be prevented
Control B. plantarii and infect caused rice shoot damping-off after paddy rice.
5 2- sulfydryl -5- methyl isophthalic acids, efficiency test of the 3,4- thiadiazoles to infection B.plantarii rice seedlings
Operating procedure is according to " preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles to infection B.plantarii rice seedlings
Experiment " is carried out, and test compound only is changed into 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles.
2- sulfydryl -5- the methyl isophthalic acids of table 3., preventive effect of the 3,4- thiadiazoles in matrix additive process
| Processing | Root length (cm) | Stem length (cm) |
| A | 4.8 | 1.8 |
| B | 0.2 | 0.3 |
| C | 3.2** | 0.7** |
| D | 4.0** | 1.1** |
| E | 4.2** | 1.5** |
| F | 4.3 | 1.8 |
**P<0.005
As shown in Table 3, B.plantarii rice seedlings, the 2- sulfydryl -5- methyl isophthalic acids through 0.005mM-5mM, 3,4- are inoculated with
Thiadiazoles processing, disease symptom obtains significantly alleviating (P ﹤ 0.005).Illustrate 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles can have
Effect preventing and treating B.plantarii infects caused rice shoot damping-off after paddy rice.
The seed treatment of embodiment 3
Difference 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiophenes two
Azoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution (0.005mM-5mM), 0.01% dimethyl
Sulfoxide solution carries out vernalization, and the rice paddy seed after the completion of vernalization is divided into four batches.Every batch is separately added into and prepares B.plantarii
Bacteria suspension, as not inoculation group (not have the surface of the seed to be defined), cultivates 24 small as inoculation group or isometric sterilizing clear water
When.Plant respectively in MS plant cultures, 12 every bottle.And blake bottle is put into 25 DEG C of constant incubators, daily illumination 12h
Dark 12h is cultivated rice seedlings, its upgrowth situation of continuous observation, and determines at the 3rd day the length of rice seedlings root and leaf,
Carry out statistical analysis.
Preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table 4. in seed treatment
| Processing | Stem length (cm) | Root length (cm) |
| A | 4.8 | 2.1 |
| B | 1 | 0.3 |
| C | 2.8** | 0.8** |
| D | 3.5** | 1.2** |
| E | 4.1** | 1.4** |
| F | 4.9 | 1.8 |
**P<0.005
Preventive effect of the 2,5- dimercapto -1,3,4- thiadiazoles of table 5. in seed treatment
**P<0.005
2- sulfydryl -5- the methyl isophthalic acids of table 6., preventive effect of the 3,4- thiadiazoles in seed treatment
| Processing | Stem length (cm) | Root length (cm) |
| A | 4.3 | 1.8 |
| B | 0.8 | 0.3 |
| C | 2.5** | 0.5** |
| D | 2.9** | 0.7** |
| E | 3.5** | 1.2** |
| F | 4.7 | 2 |
**P<0.005
As a result as shown in Table 4-6, compare, be only inoculated with after B.plantarii with the paddy growth situation without any processing
Paddy growth be substantially suppressed, and use 2- amino -5- sulfydryls -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5-
Dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution (0.005mM-
5mM) paddy growth of seed soaking has but obtained slight promotion (P ﹤ 0.05).In addition, after inoculation B.plantarii, hair
Disease symptoms are significantly suppressed (P ﹤ 0.005).This explanation is using 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, 2,5- bis-
Sulfydryl -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution carry out seed soaking to rice paddy seed,
B. plantarii can effectively be prevented and treated and infect caused rice shoot damping-off after paddy rice.
The spraying method of embodiment 4
Paddy rice vernalization and inoculation B.plantarii be the same as Examples 2, are planted in MS plant cultures, 12 every bottle.And will training
Foster bottle is put into 25 DEG C of constant incubators, and daily illumination 12h dark 12h is cultivated rice seedlings, after 1 day, to rice seedling
Spray 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiadiazoles solution
(0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution (0.005mM-5mM), 0.01% dimethyl sulfoxide (DMSO) are molten
Liquid, and the length of rice seedlings root and leaf was determined at the 3rd day, carry out statistical analysis.
Preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table 7. in spraying method method
**P<0.005
Preventive effect of the 2,5- dimercapto -1,3,4- thiadiazoles of table 8. in spraying method
| Processing | Stem length (cm) | Root length (cm) |
| A | 4.8 | 1.9 |
| B | 0.4 | 0.2 |
| C | 4.0** | 1.2** |
| D | 4.3** | 1.6** |
| E | 4.9** | 2.1** |
| F | 4.8 | 2.2 |
**P<0.005
2- sulfydryl -5- the methyl isophthalic acids of table 9., preventive effect of the 3,4- thiadiazoles in spraying method
| Processing | Stem length (cm) | Root length (cm) |
| A | 5.1 | 2.2 |
| B | 0.2 | 0.0 |
| C | 4.2** | 1.4** |
| D | 4.5** | 1.7** |
| E | 5.0** | 2.4** |
| F | 4.9 | 2.0 |
**P<0.005
As a result as shown in table 7-9,2- amino -5- sulfydryls -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- are used
Dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution (0.005mM-
After 5mM) spraying, the disease symptom of rice seedling is significantly suppressed (P ﹤ 0.005).This explanation use 2- amino -5- sulfydryls -
1,3,4- thiadiazoles solution, 2,5- dimercapto -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution pair
Rice seedling carries out spraying processing, can effectively prevent and treat B.plantarii and infect caused rice shoot damping-off after paddy rice.
Embodiment 5 is excuted a law to water droplet
By 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,5- dimercapto -1,3,4- thiadiazoles
Solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution (0.005mM-5mM), according to every mu of drop dispenser
5 liters of drops of liquid are applied, and control group only drips the aqueous solution for applying 0.01% isometric dimethyl sulfoxide (DMSO), keep water layer one week.Paddy rice is urged
Bud and inoculation B.plantarii be the same as Examples 2.Sow, and determined at the 20th day in the rice seedling bed of above-mentioned dispenser and non-dispenser
The length of rice seedlings root and leaf, carries out statistical analysis.
Preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table 10. in being excuted a law to water droplet
**P<0.005
Preventive effect of the 2,5- dimercapto -1,3,4- thiadiazoles of table 11. in being excuted a law to water droplet
| Processing | Stem length (cm) | Root length (cm) |
| A | 18.1 | 8.5 |
| B | 4.0 | 1.5 |
| C | 13.8** | 5** |
| D | 16.5** | 6.3** |
| E | 18.4** | 8.8** |
| F | 18.3 | 8.6 |
**P<0.005
2- sulfydryl -5- the methyl isophthalic acids of table 12., preventive effect of the 3,4- thiadiazoles in being excuted a law to water droplet
| Processing | Stem length (cm) | Root length (cm) |
| A | 18.9 | 9.2 |
| B | 4.3 | 1.6 |
| C | 10.4** | 3.7** |
| D | 12.5** | 4.4** |
| E | 15.0** | 5.1** |
| F | 18.2 | 8.4 |
**P<0.005
As a result as shown in table 10-12, using 2- amino -5- sulfydryls -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,
5- dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution
After (0.005mM-5mM) is applied to water droplet, the disease symptom of rice seedling is significantly suppressed (P ﹤ 0.005).This explanation is used
2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, 2,5- dimercapto -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl isophthalic acids,
3,4- thiadiazoles solution are applied to water droplet behind processing rice seedling bed, can effectively prevent rice shoot damping-off caused by B.plantarii.
Embodiment 6 mixes sandy soil broadcast application
3 kilograms of sandy soil are spread fertilizer over the fields according to every mu, respectively with 2- amino -5- sulfydryls -1,3,4- thiadiazoles solution (0.005mM-
5mM), 2,5- dimercaptos -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution
(0.005mM-5mM) is mixed thoroughly, and control group is mixed thoroughly using 0.01% isometric dimethyl sulphoxide aqueous solution with sandy soil.After spreading fertilizer over the fields,
Keep water layer one week.Paddy rice vernalization and inoculation B.plantarii be the same as Examples 2.In the rice seedling bed of above-mentioned dispenser and non-dispenser
Sow, and the length of rice seedlings root and leaf was determined at the 20th day, carry out statistical analysis.
Preventive effect of the 2- amino -5- sulfydryl -1,3,4- thiadiazoles of table 13. in native broadcast application is mixed
| Processing | Stem length (cm) | Root length (cm) |
| A | 21.4 | 8.7 |
| B | 3.2 | 1.3 |
| C | 11.6** | 5.1** |
| D | 14.5** | 7.4** |
| E | 17.4** | 8.8** |
| F | 19.6 | 9.0 |
**P<0.005
Preventive effect of the 2,5- dimercapto -1,3,4- thiadiazoles of table 14. in native broadcast application is mixed
| Processing | Stem length (cm) | Root length (cm) |
| A | 18.8 | 8.2 |
| B | 2.6 | 1.5 |
| C | 11.0** | 4.8** |
| D | 13.2** | 5.9** |
| E | 16.9** | 8.3** |
| F | 18.2 | 8.4 |
**P<0.005
2- sulfydryl -5- the methyl isophthalic acids of table 15., preventive effect of the 3,4- thiadiazoles in native broadcast application is mixed
| Processing | Stem length (cm) | Root length (cm) |
| A | 20.2 | 9.3 |
| B | 4.4 | 2.8 |
| C | 10.5** | 5.6** |
| D | 15.5** | 6.5** |
| E | 16.4** | 8.2** |
| F | 18.9 | 8.7 |
**P<0.005
As a result as shown in table 13-15, using 2- amino -5- sulfydryls -1,3,4- thiadiazoles solution (0.005mM-5mM), 2,
5- dimercapto -1,3,4- thiadiazoles solution (0.005mM-5mM), 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles solution
(0.005mM-5mM) is mixed after sandy soil spread fertilizer over the fields, and the disease symptom of rice seedling is significantly suppressed (P ﹤ 0.005).This explanation is adopted
With 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, 2,5- dimercapto -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl -
1,3,4- thiadiazoles solution is mixed soil and spread fertilizer over the fields behind processing rice seedling bed, can effectively prevent rice shoot damping-off caused by B.plantarii.
Embodiment 7 mixes the decoction combined treatment in sandy soil broadcast application
Implementing procedure be the same as Example 6.Experiment decoction is only changed to combination solution I:2- amino -5- sulfydryl -1,3,4- thiophenes two
(both compound proportions are 1 to the combination solution of azoles and 2,5- dimercapto -1,3,4- thiadiazoles:1, concentration range is 0.005mM-
In 5mM, 0.005mM combination solution be 2- amino -5- sulfydryls -1,3 containing 0.005mM, 4- thiadiazoles and 0.005mM 2,
5- dimercapto-1,3,4-thiadiazoles, similarly hereinafter)；Combination solution II:2- amino -5- sulfydryl -1,3,4- thiadiazoles and 2- sulfydryls -5-
Methyl isophthalic acid, (both compound proportions are 1 to the combination solution of 3,4- thiadiazoles:1, concentration is 0.005mM-5mM)；Combination solution III:
2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, (both compound proportion are the combination solution of 3,4- thiadiazoles
1:1, concentration is 0.005mM-5mM).Remaining Setup Experiments process and condition are same as Example 6.
Preventive effects of the combined treatment I of table 16. in native broadcast application is mixed
| Processing | Stem length (cm) | Root length (cm) |
| A | 20.0 | 8.0 |
| B | 3.3 | 1.0 |
| C | 13.0** | 5.3** |
| D | 15.8** | 6.6** |
| E | 18.6** | 7.9** |
| F | 19.2 | 7.4 |
**P<0.005
Preventive effects of the combined treatment II of table 17. in native broadcast application is mixed
| Processing | Stem length (cm) | Root length (cm) |
| A | 20.6 | 7.8 |
| B | 4.0 | 1.3 |
| C | 12.4** | 5.2** |
| D | 14.3** | 7.0** |
| E | 17.9** | 7.5** |
| F | 18.6 | 8.2 |
**P<0.005
Preventive effects of the combined treatment III of table 18. in native broadcast application is mixed
| Processing | Stem length (cm) | Root length (cm) |
| A | 20.2 | 7.5 |
| B | 4.2 | 1.1 |
| C | 10.8** | 4.9** |
| D | 13.7** | 6.1** |
| E | 16.1** | 6.7** |
| F | 19.5 | 8.1 |
**P<0.005
As a result as shown in table 16-18, mixed respectively after sandy soil spread fertilizer over the fields using combination solution I, II, III, the morbidity of rice seedling
Symptom is significantly suppressed (P ﹤ 0.005).This explanation utilizes 2- amino -5- sulfydryl -1,3,4- thiadiazoles solution, the mercaptos of 2,5- bis-
Both any compoundings in base -1,3,4- thiadiazoles solution, 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles threes, progress is mixed soil and spread
Processing rice seedling bed is applied, can effectively prevent rice shoot damping-off caused by B.plantarii.
Finally, in addition it is also necessary to it is noted that listed above is only specific embodiment of the invention.Obviously, the present invention is not limited
In above example, there can also be many deformations.One of ordinary skill in the art can directly lead from present disclosure
All deformations for going out or associating, are considered as protection scope of the present invention.
Claims (9)
1. application of the thiadiazole compound on paddy bacterial rice shoot damping-off control medicament is prepared.
2. application as claimed in claim 1, it is characterised in that the thiadiazole compound is 2- amino -5- sulfydryls -1,3,
4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles or 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles.
3. application as claimed in claim 1, it is characterised in that the paddy bacterial rice shoot damping-off is by burkholderia
B.plantarii causes.
4. a kind of pesticide control, it is characterised in that active ingredient is thiadiazole compound.
5. pesticide control as claimed in claim 4, it is characterised in that the thiadiazole compound be 2- amino -5- sulfydryls -
At least one of 1,3,4- thiadiazoles, 2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles.
6. pesticide control as claimed in claim 4, it is characterised in that the concentration of the thiadiazole compound is 0.005~
5mM。
7. pesticide control as claimed in claim 5, it is characterised in that the active ingredient is 2- amino -5- sulfydryls -1,3,4-
Thiadiazoles and 2,5- dimercapto-1,3,4-thiadiazole, its concentration is respectively 0.005~5mM and 0.005~5mM；Or, it is described
Active ingredient is 2- amino -5- sulfydryls -1,3,4- thiadiazoles and 2- sulfydryl -5- methyl isophthalic acids, 3,4- thiadiazoles, and its concentration is respectively
0.005~5mM and 0.005~5mM；Or the active ingredient is 2,5- dimercapto -1,3,4- thiadiazoles and 2- sulfydryl -5- first
Base -1,3,4- thiadiazoles, its concentration is respectively 0.005~5mM and 0.005~5mM.
8. pesticide control as claimed in claim 4, it is characterised in that be calculated in mass percent, including following components：
Thiadiazole compound 0.006~0.142%；
Dimethyl sulfoxide (DMSO) 0.010%；
Ultra-pure water 99.848~99.9839%.
9. a kind of method for preventing and treating paddy bacterial rice shoot damping-off, it is characterised in that rice paddy seed is soaked in into right such as will
Ask in 4~8 any described pesticide controls；Or, pesticide control drop is imposed on into rice seedling bed；Or, by the pesticide control
Sandy soil are mixed to spread fertilizer over the fields in rice seedling bed；Or to spraying the pesticide control in budding rice seedling.
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| CN114375716A (en) * | 2022-01-19 | 2022-04-22 | 青海省农林科学院 | Highland barley streak germ liquid infection identification method |
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