CN107232083B - Automatic record variable speed rotation diving platform experimental apparatus - Google Patents
Automatic record variable speed rotation diving platform experimental apparatus Download PDFInfo
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- CN107232083B CN107232083B CN201710428928.3A CN201710428928A CN107232083B CN 107232083 B CN107232083 B CN 107232083B CN 201710428928 A CN201710428928 A CN 201710428928A CN 107232083 B CN107232083 B CN 107232083B
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- 230000009189 diving Effects 0.000 title claims abstract description 36
- 238000012806 monitoring device Methods 0.000 claims abstract description 11
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 43
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 22
- 241000699670 Mus sp. Species 0.000 claims description 18
- 208000002173 dizziness Diseases 0.000 claims description 11
- 229910052742 iron Inorganic materials 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims 1
- 238000002474 experimental method Methods 0.000 description 23
- 230000002929 anti-fatigue Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000003814 drug Substances 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 229940079593 drug Drugs 0.000 description 4
- 230000002045 lasting effect Effects 0.000 description 4
- 230000001362 anti-vertigo Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 206010016275 Fear Diseases 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 239000000935 antidepressant agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- GJMUCSXZXBCQRZ-UHFFFAOYSA-N geraniin Natural products Oc1cc(cc(O)c1O)C(=O)OC2OC3COC(=O)c4cc(O)c(O)c(O)c4c5cc(C(=O)C67OC3C(O6)C2OC(=O)c8cc(O)c(O)c9OC%10(O)C(C(=CC(=O)C%10(O)O)C7=O)c89)c(O)c(O)c5O GJMUCSXZXBCQRZ-UHFFFAOYSA-N 0.000 description 1
- 229930194078 geranin Natural products 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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Abstract
An automatic recording variable-speed rotating diving platform experimental device is characterized in that the bottom end of a main rotating shaft is positioned in a funnel, the bottom end of the main rotating shaft is connected with a driving device, and the driving device is connected with a controller; a spherical crown diving platform for a mouse to grasp is fixed at the top end of the main rotating shaft; the main rotating shaft is also provided with a speed measuring device for detecting the rotating speed of the main rotating shaft; the speed measuring device comprises a speed measuring rotary table coaxially and fixedly arranged on the main rotating shaft, a speed measuring magnetic block is fixed on the speed measuring rotary table, and the speed measuring rotary table further comprises a Hall sensor fixedly arranged on one side of the main rotating shaft, the Hall sensor and the speed measuring magnetic block are vertically opposite and matched with each other, and the distance between the Hall sensor and the main rotating shaft is equal to the distance between the speed measuring magnetic block and the main rotating shaft; the Hall sensor is connected with the controller. A monitoring device for detecting whether the mouse falls off or not is arranged at the small opening of the funnel; the monitoring device comprises a plurality of pairs of infrared correlation sensors arranged on the inner wall of the funnel, and each pair of infrared correlation sensors is connected with the controller; and a timer is arranged in the controller.
Description
Technical Field
The invention relates to an automatic recording variable-speed rotating diving platform experimental device.
Background
The experiment mouse does unsupported passive rotation movement on a rotating platform, and the persistence of the passive rotation of the mouse is observed at different rotating speeds, which can be considered as the experiment belonging to the anti-fatigue or anti-vertigo experiment of the mouse (see Geranin, Yuanyong, Yinhuazhong and the like. the development of a data memory animal fatigue tester [ J ]. China comparison medical journal, 2006, 16 (8): 489-.
Disclosure of Invention
In order to solve the defects in the background art, the invention provides an automatic recording variable-speed rotating diving platform experimental device.
The technical scheme for solving the problems is as follows:
an automatic recording variable-speed rotating diving platform experimental device comprises a funnel for receiving a falling mouse, wherein the funnel is erected in a hanging manner, the upper end and the lower end of the funnel are both provided with openings, the large opening faces upwards, and the small opening faces downwards; a main rotating shaft extending along the central shaft direction of the funnel is arranged in the funnel, the bottom end of the main rotating shaft is positioned in the funnel, the bottom end of the main rotating shaft is connected with a driving device for driving the main rotating shaft to rotate, and the driving device is connected with a controller; the top end of the main rotating shaft extends upwards and is positioned above the funnel, and a spherical crown diving platform for a mouse to grasp is fixed on the top end of the main rotating shaft;
the main rotating shaft is also provided with a speed measuring device for detecting the rotating speed of the main rotating shaft; the speed measuring device comprises a speed measuring rotary table coaxially and fixedly arranged on the main rotating shaft, a speed measuring magnetic block is fixed on the speed measuring rotary table, and the speed measuring rotary table further comprises a Hall sensor fixedly arranged on one side of the main rotating shaft, the Hall sensor and the speed measuring magnetic block are vertically opposite and matched with each other, and the distance between the Hall sensor and the main rotating shaft is equal to the distance between the speed measuring magnetic block and the main rotating shaft; the Hall sensor is connected with the controller, the Hall sensor transmits the collected information of the speed measuring magnetic block to the controller, the controller processes the received information of the speed measuring magnetic block from the Hall sensor into the rotating speed information of the main rotating shaft, and the rotating speed is displayed on a control panel of the controller;
a monitoring device for detecting whether the mouse falls off is arranged at the small opening of the funnel; the monitoring device comprises a plurality of pairs of infrared correlation sensors arranged on the inner wall of the funnel, each pair of infrared correlation sensors are oppositely arranged on the inner wall of the funnel, and each pair of infrared correlation sensors are connected with the controller; and a timer is arranged in the controller.
Furthermore, the funnel is erected on the horizontal working surface through the upright post.
Furthermore, the driving device is a speed reducing motor, and an output shaft of the speed reducing motor is coaxially connected with the bottom end of the main rotating shaft through a coupler.
Further, the reduction motor is fixed through an iron stand, and the iron stand is located at the center of the funnel.
Further, the Hall sensor is fixed on the iron stand through a support and is positioned below the speed measuring magnetic block; the speed measuring magnetic block is fixed on the lower surface of the edge of the speed measuring rotary table.
Furthermore, the top surface of the diving platform is a plane and is circular.
Further, monitoring devices includes that the equidistance sets up 2 pairs of infrared correlation sensors on the funnel inner wall.
The technical conception of the invention is as follows:
the experiment mouse does unsupported passive rotation movement on a rotating platform, and the persistence of the passive rotation of the mouse is observed at different rotating speeds, and the experiment can be considered as belonging to an anti-fatigue or anti-dizziness experiment of the mouse and can be used for research of anti-fatigue drugs, anti-dizziness drugs, antidepressant drugs and exciting drugs and research of related experiments of medicine and aerospace.
When a mouse is on a diving platform with a certain height, the mouse can not jump down temporarily due to terrorism, and can gradually become anxious or dizzy along with the variable speed rotation of the diving platform. The duration of the passive rotation of the mouse on the diving platform at different rotating speeds can be used for evaluating the drug effect and the related medical effect of related developed drugs, the rotating motion effect in aerospace and the like.
Experimentally, it is observed that the mice rotated at a certain height have a commonality: the limbs and the tails are used for searching for the support, and the spherical crown-shaped diving platform is designed in such a way to eliminate the experimental interference factors.
The invention has the following beneficial effects:
the invention is adopted to test the anti-fatigue and anti-vertigo capability of animals, reports are not found at home and abroad at present, similar animal anti-fatigue and anti-vertigo experiments are adopted in the industry at present, and a standing line type, a standing rod type (Geernin, Yogyuan, Yinhuai and the like; the development of a data memory animal fatigue tester [ J ]. China journal of comparative medicine, 2006, 16 (8): 489-490.), a hanging rod type (Heynen, Geernin, Shengzhenhua and the like; the development of a self-recording animal hanging rod durability test device [ J ]. the China journal of comparative medicine, 2014, 24 (7): 77-80.), a turnover type holding rod rotation type and the like are adopted, and the invention is different from the previous devices: the mouse is passively rotated on the spherical crown diving platform without a support station, and the anti-fatigue and anti-dizziness experiment is carried out in the posture. Animals such as mice and the like which are commonly used in experiments have limited anti-fatigue and anti-dizziness capability under the unsupported condition, the time of one passive rotation is not long, and the experimental observation of a single mouse is generally not more than 30 min.
The device automatically records the passive rotation duration of the mouse at different rotating speeds. The detection mode and the design idea have certain innovativeness and advancement. As a brand-new experimental method and concept in the multidisciplinary field, and an automatic detection means is matched, the method has an optimistic prospect of popularization and application in the industry.
Drawings
FIG. 1 is a schematic structural view of the present invention; wherein, a, b and c represent connectors.
FIG. 2 shows the results of a passive spin duration experiment of 36 mice at different spin speeds; wherein the vertical axis represents rotation time (time) in min (minutes), and the horizontal axis represents rotation speed (speed) in revolutions per minute (R/min).
Wherein: 1. the device comprises an iron support, a speed reducing motor 2, a coupler 3, a Hall sensor 4, a speed measuring magnetic block 5, a speed measuring rotary table 6, a main rotating shaft 7, a diving platform 8, a funnel 9, a controller 10 and an infrared correlation sensor 11.
Detailed Description
Referring to the attached drawings, the experiment device for automatically recording the variable-speed rotating diving platform comprises a funnel 9 for receiving a falling mouse, wherein the funnel 9 is erected in a suspended mode, the upper end and the lower end of the funnel 9 are both provided with openings, the large opening faces upwards, and the small opening faces downwards; a main rotating shaft 7 extending along the central axis direction of the funnel 9 is arranged in the funnel 9, the bottom end of the main rotating shaft 7 is positioned in the funnel 9, the bottom end of the main rotating shaft 7 is connected with a driving device for driving the main rotating shaft 7 to rotate, and the driving device is connected with a controller 10 (connected through a connecting head a); the top end of the main rotating shaft 7 extends upwards and is positioned above the funnel 9, and a spherical crown diving platform 8 for the mouse to grasp is fixed on the top end of the main rotating shaft 7;
the main rotating shaft 7 is also provided with a speed measuring device for detecting the rotating speed of the main rotating shaft 7; the speed measuring device comprises a speed measuring rotary table 6 coaxially and fixedly arranged on a main rotating shaft 7, a speed measuring magnetic block 5 is fixed on the speed measuring rotary table 6, and the speed measuring rotary table further comprises a Hall sensor 4 fixedly arranged on one side of the main rotating shaft 7, wherein the Hall sensor 4 is vertically opposite to and matched with the speed measuring magnetic block 5, and the distance between the Hall sensor 4 and the main rotating shaft 7 is equal to the distance between the speed measuring magnetic block 5 and the main rotating shaft 7; the Hall sensor 4 is connected with the controller 10 (connected through the connecting head b), the Hall sensor 4 transmits the collected information of the speed measuring magnetic block to the controller 10, the controller 10 processes the received information of the speed measuring magnetic block from the Hall sensor 4 into the rotating speed information of the main rotating shaft 7, and the rotating speed is displayed on a control panel of the controller 10 in real time;
a monitoring device for detecting whether the mouse falls off is arranged at a small opening of the funnel 9; monitoring devices is including setting up a plurality of pairs of infrared correlation sensor 11 on the inner wall of funnel 9, and every pair of infrared correlation sensor 11 sets up relatively on the inner wall of funnel 9, and every pair of infrared correlation sensor all links to each other (is connected through connecting head c) with controller 10, and has the timer in the controller 10. After the controller 10 drives the driving means to start rotating, a timer is started at an appropriate time as required to start timing, and the timer can also be reset as required, and after the infrared correlation sensor 11 transmits the detected mouse drop information to the controller 10, the controller 10 drives the timer to stop timing, records and saves the timing information, and displays it on the control panel.
The funnel 9 is erected on a horizontal working surface through a stand column.
The driving device is a speed reducing motor 2, and an output shaft of the speed reducing motor 2 is coaxially connected with the bottom end of the main rotating shaft 7 through a coupler 3.
The speed reduction motor 2 is fixed by an iron stand 1, and the iron stand 1 is located at the center of the funnel 9.
The Hall sensor 4 is fixed on the iron stand 1 through a support, and the Hall sensor 4 is positioned below the speed measuring magnetic block 5; the speed measuring magnetic block 5 is fixed on the lower surface of the edge of the speed measuring rotary disc 6.
The top surface of the diving platform 8 is a plane and is circular.
The monitoring device comprises 2 pairs of infrared correlation sensors 11 which are arranged on the inner wall of the funnel 9 at equal intervals.
1.1 design of the rotating Unit
The speed reduction motor 2 is fixed in the upper end of iron stand platform 1, connect the main pivot 7 that the diameter is 4mm length for 300mm through shaft coupling 3, the top of main pivot 7 is connected the spherical crown shape diving tower 8 that the diameter is 80mm, the diving tower mesa is circular, the diameter is 55mm, the experimental mouse that just is enough about 25g stands steadily, diving tower plane height is 550mm, the experimental mouse fears basically and jumps down at this height, the purpose of 80mm spherical crown shape diving tower design makes the tail of mouse unable to collude main pivot 7, so, can make the mouse be in no support state completely, only lean on the appearance of standing and endure fatigue and dizzy.
The rotating speed of the diving platform 8 driven by the speed reducing motor 2 is controlled by a rotating speed adjusting knob on a control panel of the controller 10.
1.2 Hall speed measuring unit design
The diameter is 40 mm's the carousel 6 vertical fixation that tests the speed on main pivot 7, and be located 3 top positions of shaft joint, the magnetic path 5 that tests the speed is fixed at the edge of carousel 6 that tests the speed, hall sensor 4 is fixed in the pole setting of brandreth platform 1, be located the position of 2 below of gear motor, hall sensor 4 corresponds with carousel 6 that tests the speed perpendicularly, see figure 1, so, when main pivot 7 drives carousel 6 that tests the speed and rotates, hall sensor 4 measures real-time rotational speed and shows in real time on the panel of controller 10.
1.3 Signal receiving Unit design
The unit consists of a large-caliber funnel 9 made of a smooth acrylic plate and two pairs of infrared correlation sensors 11 and is used for receiving a mouse falling from a diving platform 8 and determining the passive rotation lasting time of the mouse. Because the mice standing on the spherical crown diving platform 8 and rotating passively jump out or fall out, the throwing distance is not large, and the upper large opening of the funnel is designed to be 500 multiplied by 500mm according to the estimation of experiments2The lower small opening is 90 multiplied by 90mm2The slope angle of the inner wall of the funnel is 45 degrees, and two pairs of infrared correlation sensors 11 are uniformly arranged at the lower opening at intervals of 30 mm. Therefore, when a mouse falls into the funnel from any angle, the mouse can rapidly slide to the lower opening along the smooth inner wall of the funnel and fall out of the funnel, the two infrared sensors 11 capture the falling signal of the mouse and transmit the signal to the controller, and the controller controls the timer to stop timing.
1.4 control Unit design
The controller 10 may supply power to the deceleration motor 2, the infrared correlation sensor 11, and the hall sensor 4, and control the deceleration motor 2, the infrared correlation sensor 11, and the hall sensor 4 to be turned on. The controller 10 can receive the information transmitted by the infrared correlation sensor 11 and the Hall sensor 4, and process and display the information.
And a rotating speed display and a timing display are arranged on the control panel to respectively display the rotating speed and the rotating time of the mouse.
The control panel is also provided with a speed regulating knob for driving the controller 10 to regulate the rotating speed of the speed reducing motor;
the control panel is also provided with a zero clearing button for driving the timer to clear and a timing button for driving the timer to start timing;
the control panel is also provided with a start/stop button for driving the speed reduction motor to start or stop rotating;
the control panel is also provided with a start/stop switch for starting the operation of the drive controller 10 and stopping the operation of the drive controller 10.
2. The experimental steps are as follows:
2.1, moving the funnel 9 to enable the central axis to coincide with the main rotating shaft 7, connecting a power supply (220V, 50Hz) of the controller 10, automatically resetting the timing display on the control panel at the moment, enabling the rotating speed display to return to zero, and waiting for two starting signals to arrive.
2.2, the mice are taken and placed on a diving platform 8, and after stabilization, the experiment is started. Turning on a start/stop switch, and starting the controller to work; press and start/stop the button, the deceleration motor begins the rotation, and the rotational speed regulation knob, rotate with the low-speed of 30 rounds/minute earlier, the jump platform can be hugged closely because of fear to the mouse, slowly accelerate to required experimental speed, press the timing button, several minutes to ten minutes after, experimental mouse is because of fatigue or dizzy to the body force not enough, jump out jump platform 8 or four limbs freely fall after out of control, again along the smooth funnel osculum that is equipped with infrared correlation sensor 11 of funnel 9 inner wall, a timer on the controller panel stops the timing this moment, and the timing display shows current timing value in real time, an experimental process finishes.
2.3, pressing a zero clearing button, and completely clearing the timing data; pressing the start/stop button again to stop the rotation of the diving platform; the start/stop switch is closed and the controller 10 stops operating.
3. Results of the experiment
36 ICR mice, female, with the weight of 20 +/-2 g, were divided into two groups, the first group of 18 mice fed normally, the second group of 18 mice fed no food for 1 day, and only fed water.
The first 18 mice experiments were performed at three rotational speed levels of 50 rpm, 70 rpm and 90 rpm, with 6 mice per rotational speed level. A second group of 18 mice was subsequently tested in the same manner.
The experiment is carried out according to the experimental steps, the experimental device can automatically record according to the passive rotation time of the mouse, the experimental time of one mouse can be determined according to the physical ability and the dizziness degree of the mouse, the lasting time of the passive rotation of the robust mouse can be ten minutes, and the mouse can fall off in less than 5 minutes due to poor physical ability or dizziness resistance.
Fig. 2 shows the results of the passive spin duration experiments in 36 mice at different spin speeds.
From the experimental results, the difference of the passive rotation lasting time of two groups of mice is obvious no matter in the high-speed and low-speed states, and the difference of the passive rotation lasting time of the same group of mice in the high-speed and low-speed states is also obvious, so that the applicability of the experimental method and the device in the anti-fatigue and anti-dizziness test of the mice is fully demonstrated. In the experiment, a plurality of mice are resistant to rotation occasionally, the continuous passive rotation time is long, few mice are not suitable for the platform-type rotation environment, and most experiment results are ideal.
When the mouse is placed, the mouse needs to be rotated after standing on the diving platform 8 so as to avoid falling by mistake.
This device need work under the environment that is quiet steady relatively, vibrate and can make the mouse fall too early after receiving the frightening with the environment that excessively makes noise, influence the experiment effect. The instrument can be operated continuously for 48 hours without any interference from unexpected conditions (such as power failure).
The embodiments described in this specification are merely illustrative of implementations of the inventive concept and the scope of the present invention should not be considered limited to the specific forms set forth in the embodiments but includes equivalent technical means as would be recognized by those skilled in the art based on the inventive concept.
Claims (6)
1. The utility model provides an automatic record variable speed rotation diving tower experimental apparatus which characterized in that: the mouse falling device comprises a funnel for receiving falling mice, wherein the funnel is erected in a suspension manner, the upper end and the lower end of the funnel are both provided with openings, the large opening is upward, and the small opening is downward; a main rotating shaft extending along the central axis direction of the funnel is arranged in the funnel, the bottom end of the main rotating shaft is positioned in the funnel, the bottom end of the main rotating shaft is connected with a driving device for driving the main rotating shaft to rotate, and the driving device is connected with a controller; the top end of the main rotating shaft extends upwards and is positioned above the funnel, and a spherical crown diving platform for a mouse to grasp is fixed on the top end of the main rotating shaft; the top surface of the diving platform is a plane, is circular and has the diameter of 55mm, so that the mouse can bear fatigue and dizziness only by standing;
the main rotating shaft is also provided with a speed measuring device for detecting the rotating speed of the main rotating shaft; the speed measuring device comprises a speed measuring rotary table coaxially and fixedly arranged on the main rotating shaft, a speed measuring magnetic block is fixed on the speed measuring rotary table, and the speed measuring rotary table further comprises a Hall sensor fixedly arranged on one side of the main rotating shaft, the Hall sensor and the speed measuring magnetic block are vertically opposite and matched with each other, and the distance between the Hall sensor and the main rotating shaft is equal to the distance between the speed measuring magnetic block and the main rotating shaft; the Hall sensor is connected with the controller, the Hall sensor transmits the collected information of the speed measuring magnetic block to the controller, the controller processes the received information of the speed measuring magnetic block from the Hall sensor into the rotating speed information of the main rotating shaft, and the rotating speed is displayed on a control panel of the controller;
a monitoring device for detecting whether the mouse falls off is arranged at the small opening of the funnel; the monitoring device comprises a plurality of pairs of infrared correlation sensors arranged on the inner wall of the funnel, each pair of infrared correlation sensors are oppositely arranged on the inner wall of the funnel, and each pair of infrared correlation sensors are connected with the controller; and a timer is arranged in the controller.
2. An automatic recording variable speed rotating diving tower experimental device as claimed in claim 1, characterized in that: the funnel is erected on the horizontal working face through the upright post.
3. An automatic recording variable speed rotating diving tower experimental device as claimed in claim 2, characterized in that: the driving device is a speed reducing motor, and an output shaft of the speed reducing motor is coaxially connected with the bottom end of the main rotating shaft through a coupler.
4. An automatic recording variable speed rotating diving tower experimental device as claimed in claim 3, characterized in that: the speed reduction motor is fixed through an iron stand platform, and the iron stand platform is located at the center of the funnel.
5. An automatic recording variable speed rotating diving tower experimental device as claimed in claim 4, characterized in that: the Hall sensor is fixed on the iron stand through a support and is positioned below the speed measuring magnetic block; the speed measuring magnetic block is fixed on the lower surface of the edge of the speed measuring rotary table.
6. An automatic recording variable speed rotating diving tower experimental device as claimed in claim 5, characterized in that: the monitoring device comprises 2 pairs of infrared correlation sensors which are arranged on the inner wall of the funnel at equal intervals.
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| CN205758761U (en) * | 2016-06-15 | 2016-12-07 | 浙江中医药大学 | A kind of rat restraint device in model construction |
| CN106706869B (en) * | 2017-01-22 | 2019-02-15 | 浙江中医药大学 | A kind of multichannel mouse vertical rotary persistence test device |
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