CN107231947A - The culture medium and method of a kind of energy large-scale planting pixie stool - Google Patents
The culture medium and method of a kind of energy large-scale planting pixie stool Download PDFInfo
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Abstract
The invention discloses a kind of culture medium of energy large-scale planting pixie stool and method, the culture medium, including mother culture media, original seed, Cultivar culture medium and pixie stool planting material；Wherein mother culture media includes water, dry Cortex Cunninghamiae Lanceolatae, dry robur wood chip, wheat bran, potato, glucose, manganese sulfate, agar；Original seed, Cultivar culture medium include weed tree sawdust, little particle corncob, wheat bran, Chinese fir wood chip, land plaster, manganese sulfate；Pixie stool planting material includes weed tree sawdust, robur leaf, little particle corncob, wheat bran, Chinese fir wood chip, land plaster, fertile soil, manganese sulfate.Wild pixie stool is carried out tissue separation by the present invention, cultivate parent species, parent species are accessed into original seed again, original seed, which is linked into Cultivar culture medium, is trained cultigen, cultigen is seeded into pixie stool compost again and produces pixie stool, technique is easy, pollution rate is low, strain is pure, simple to operate, and cultivation period is short, and biological efficiency reaches 70%.
Description
Technical field
The present invention relates to fungus growing technique, the culture medium and method of specifically a kind of energy large-scale planting pixie stool.
Background technology
Pixie stool is one of big famous edible mycorrhizal fungi in the world six, pixie stool (CantharelluscibariusFr),
Alias:Yellow silk bacterium, egg yolk, apricot bacterium, chrysanthemum bacterium, volt mushroom etc..Classification position is fungus circle, Basidiomycotina, Cantharellus
Cantharellus.Pixie stool fructification meat apricot, yolk yellow or loquat yellow, high 4 ~ 12cm；Cap curls inward at initial stage is in
Convex-type, rear expansion holds high to form middle recessed, flower-shape of such as leading a cow, 3 ~ 10cm of diameter, surface is smooth, slightly glues, side due to edge
Edge is thick and blunt, in wavy, involute and often have valve to split, and lamella is narrow, rib ridge shape, arranges sparse, slightly curved song, mutually bifurcated or friendship
Mistake, life is prolonged with stem；Stem is short and thick, the thick 5 ~ 8mm of long 2 ~ 6cm, tapered narrow downwards, smooth, real in meat, bacterial context yellow, thickness 2
~ 3mm, there is strong almond, micro- sweet tea；Summer and autumn is more common in, the pin broad-leaf forest being often born on well-drained gentle slope is on the ground, such as loose
Below the lower rotten pine tag of tree or under Quercus acutissima from the grass, all living creatures or closely grow thickly.
Pixie stool is with famous rich in vitamin A, according to data, protein content 21.4% in pixie stool, crude fat 4.3%,
Carbohydrate 65.7%, cellulose 11.2%, containing 18 kinds of amino acid, wherein 8 kinds of amino acid contents needed by human are very high, is
Preferable nutritional health food.According to modern medical analysis, effect of pixie stool tool flight against senium of human body and treatment are a variety of because lacking dimension
Raw element disease caused by A.
Up to the present, pixie stool production is still in artificial domesticating cultivation experimental stage, though pixie stool artificial cultivation is accidental
Report, but due to also immature in technical elements, without large-scale production.The mycelium obtained is separated from fructification, it is also difficult to
With classical its true and false of tieback method validation, pixie stool commodity can only come from wild resource, thus the market demand of pixie stool and
Supply is extremely uneven.The present invention can not only alleviate pixie stool supply shortage problem, and its wide industrialization plantation prospect will
Considerable economic benefit can be brought to peasant, therefore, significant is studied to pixie stool artificial cultivation.
Domestic patent database also discloses that some pixie stool cultivation techniques, for example：
For example：【Title】A kind of wild pixie stool artificial domesticating cultivation method,【Application number】CN201510783950.0,【Application
Day】2015-11-16, discloses a kind of wild pixie stool artificial domesticating cultivation method, and its method is：
（1）Isolation and Culture：Wild pixie stool fructification is gathered, 1000~1500ml of filtrate is taken after cleaning, plus sucrose 15~
20g, 15~20g of yeast extract, 1.5~2.0g of potassium dihydrogen phosphate, 0.5~1.0g of magnesium sulfate, 20~25g of agar, phosphoric acid buffer
Liquid adjusts pH to be 6~8, incubated in 20 DEG C after bevel inoculation, until mycelia covers with inclined-plane；
（2）Primary spawn：Parent species are inoculated into Tube propagation and are based on lucifuge culture at 25~28 DEG C, mycelia is covered with bottom of bottle i.e.
Can；Described Tube propagation base：60~100g of weed tree sawdust, beans stalk (crushing) 15~30g, 1~10g of gypsum, 10~20g of wheat bran,
2~8g of calcium superphosphate, sugarcane ward off 5~10g, 5~10g of urea, 0.2~1.0g of vitamin B, 10~15g peptones, and constant volume exists
1000ml, pH5~6,0.1~0.15, at 120~130 DEG C with 20~30min of autoclaving, described parent species inoculation
Measure as the 5-10% of Tube propagation base (siccative) weight；
（3）Cultigen culture：Parent species are cultivated into original seed be inoculated in planting material and be placed in 25~35 DEG C, 100~120r/min's
Under the conditions of 7~10d of shaking table culture, sowed；The planting material is Bag Material culture medium:Corn flour 40-60g, sucrose 20-40g,
K2PO44-8g、MgSO42-5g, agar 2-10g, add water to 1000mL, pH7.2；Described parent species inoculum concentration is Bag Material culture medium
The 10-15% of (siccative) weight；
（4）Stone is sowed：The rectangular mushroom beds of long 80cm × wide 60cm are laid, thickness 6cm planting materials compacting is first spread, removes and broadcast one
Layer cultigen, repaves material 4cm thickness sowing compactings, every square metre of application rate is the 5-10% of cultivation siccative weight, is after planting gently covered
Thin film, treats fruiting；The planting material is：Cotton seed hull 48-60g, wheat bran 18-30g, wood chip 30-50g, precipitated calcium carbonate 2-
In 5g, lime 2-10g, urea 1-5g, thiophanate 0.5-2.0g, phenol solution 15-20ml, sucrose 1-10g, water 1000ml, pH
Property；Described planting material is prepared：The raw material of above parts by weight is well mixed, is fitted into 25cm × 25cm bags, is compacted, sealing
Sack, detects bag temperature at any time, and when material temperature is up to 65 DEG C of holding 24h, opening carries out being cooled to 45 DEG C, carries out stone；
（5）Management of producing mushroom is conventional cultivation method.
Again for example：【Title】A kind of preparation method of liquid strain of chanterelle,【Application number】201210095414.8 【Shen
Please day】2012-04-01, discloses a kind of preparation method of liquid strain of chanterelle, and its method is：
（1）It is prepared by culture medium：150~250g of pine needle, 150~250g of potato are cut into small pieces, plus 1000ml water boil 20~
40min, filters off and 0.05~0.15g of calcium chloride, 0.45~0.55g of ammonium chloride, magnesium sulfate is added in pine needle and potato residue, filtrate
0.25~0.35g, 0.8~1.2g of potassium dihydrogen phosphate, 0.008~0.012g of iron chloride, 0.45~0.55g of glucose, vitamin
B10.045~0.055mg, 4~6g of peptone, 15~25g of 8~12g of beef extract and urea, are settled to 1000ml, 0.1~
0.15th, 25~35min of autoclaving is used at 118~125 DEG C, 25~35 DEG C of incubator cultures, the training of asepsis growth are placed in
Support base and retain standby；
（2）Inoculation：Pixie stool cap and the chicken fat hyphostroma of stem joint is taken to be inoculated with into step（1）Prepared culture medium
In；
（3）Spawn incubation：Culture medium after inoculation 7~10d of shaking table culture under conditions of 26~30 DEG C, 100~120r/min,
Produce liquid strain of chanterelle.
It is preferred that, the culture medium is prepared as：Pine needle 200g, potato 200g are cut into small pieces, plus 1000ml water boils
30min, filters off and calcium chloride 0.1g, ammonium chloride 0.5g, magnesium sulfate 0.3g, biphosphate is added in pine needle and potato residue, filtrate
Potassium 1g, iron chloride 0.01g, glucose 0.5g, vitamin B10.05mg, peptone 5g, beef extract 10g and urea 20g, constant volume
To 1000ml, autoclaving 30min is used at 0.103MPa, 121 DEG C, 30 DEG C of incubator culture 24h, asepsis growth are placed in
Culture medium retain it is standby.
Further, in the preparation method of foregoing liquid strain of chanterelle, when prepared by culture medium, it is being settled to 1000ml
Before, 8~12g of wheat is added, 10g wheats are preferably added to.During shaking table culture, mycelia apposition growth, can around wheat
To shorten incubation time.
It is preferred that, Spawn incubation is described in preceding method：Culture medium after inoculation is under conditions of 28 DEG C, 110r/min
7~10d of shaking table culture, produces liquid strain of chanterelle.
Compared with prior art, chicken fat hyphostroma is inoculated in shaking table culture, gained liquid spawn in the culture medium of uniqueness
The middle mycelial dry weight of pixie stool reaches 183.8mg/200ml, compares commonsense method（145.2mg/200ml）The heavy 38.6mg/ of processing
200ml, the inventive method technique is easy, pollute less, strain is pure, simple to operate, cultivation cycle is short（Cultivation cycle only has 7~10
My god）, gained liquid spawn is inoculated in cultivating bag, then pseudo-wild cultivating produces pixie stool, it is mycelial after inoculation to sprout
Rate is 80.3%, compares commonsense method（56.5%）It is high by 23.8%, it is possible to achieve scale, industrialization production, it can in addition contain in culture
Wheat is added in base, during shaking table culture, mycelia apposition growth is around wheat, so as to further effectively shorten culture
Time.
Again for example：【Title】A kind of compound method of pixie stool compost【Application number】201110161835.1 【Application
Day】2011-06-08, discloses a kind of compound method of pixie stool compost, and its compound method is：Dried poultrymanure 35%-
55%th, duck excrement 25%-45%, wheat bran skin 10%-16%, plant protein powder 1.5%-2%, limewash 0.5%-1%, wheat straw are done
It is powder 0.5%-1%, gypsum 0.5%-1%, rice straw powder 1.5%-2%, rice bran 0.1%-0.3%, yeast 0.1%-0.3%, many
Azoles 0.1%-0.3%, calcium powder 0.5%-0.8% composition are imitated, described percentage is weight percentage.
But there is variety of raw material needed for pixie stool bacterium culture medium and plant formulation in above pixie stool cultivation technique
Many, strain complex manufacturing, equipment investment is big, and planting material carbon-nitrogen ratio and Trace Elements are not suitable for chicken fat bacteria growing and height
Production, pixie stool cultivation technique is without quantitative target and specific implementation method, it is also difficult to use its true and false of the tieback method validation of classics, because
This is highly desirable to a kind of simple and practical pixie stool cultivation technique of research and development, and preferably solving pixie stool artificial cultivation does not have scale
The problem of changing cultivation.
The content of the invention
It is an object of the invention to provide a kind of culture medium of energy large-scale planting pixie stool and method, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of culture medium of energy large-scale planting pixie stool, including mother culture media, original seed, Cultivar culture medium and pixie stool are planted
Training material；Wherein mother culture media：Following raw material is added in per 2000ml water：Dry Cortex Cunninghamiae Lanceolatae 45-55g, dry robur wood chip 45-55g,
Wheat bran 15-25g, potato 180-220g, glucose 15-25g, manganese sulfate 0.05-0.15g, agar 15-25g；
Original seed, Cultivar culture medium, include according to the raw material of parts by weight：55-65 parts of weed tree sawdust, 10-20 parts of little particle corncob,
10-20 parts of wheat bran, 5-10 parts of Chinese fir wood chip, 1-3 parts of land plaster, 0.02-0.1 parts of manganese sulfate；
Pixie stool planting material, includes according to the raw material of parts by weight：45-55 parts of weed tree sawdust, 5-15 parts of robur leaf, little particle corncob
10-20 parts, 10-20 parts of wheat bran, 5-10 parts of Chinese fir wood chip, 1-3 parts of land plaster, 3-7 parts of fertile soil, 0.02-0.1 parts of manganese sulfate.
It is used as further scheme of the invention：Mother culture media：Following raw material is added in per 2000ml water：Dry Cortex Cunninghamiae Lanceolatae
50g, dry robur wood chip 50g, wheat bran 20g, potato 200g, glucose 20g, manganese sulfate 0.1g, agar 20g.
It is used as further scheme of the invention：Original seed, Cultivar culture medium, include according to the raw material of parts by weight：Weed tree sawdust
60 parts, 15 parts of little particle corncob, 15 parts of wheat bran, 8 parts of Chinese fir wood chip, 2 parts of land plaster, 0.05 part of manganese sulfate.
It is used as further scheme of the invention：Pixie stool planting material, includes according to the raw material of parts by weight：50 parts of weed tree sawdust,
10 parts of robur leaf, 15 parts of little particle corncob, 15 parts of wheat bran, 8 parts of Chinese fir wood chip, 2 parts of land plaster, 5 parts of fertile soil, manganese sulfate
0.05 part.
It is used as further scheme of the invention：The manufacturing process of mother culture media is as follows：Weigh dry Cortex Cunninghamiae Lanceolatae, dry robur wood
Bits, wheat bran, potato, glucose, manganese sulfate, agar, water, first by dry Cortex Cunninghamiae Lanceolatae, dry robur wood chip, wheat bran gauze wrapped,
Insert in reactor, add water, keep 10min after water is boiled, be then placed in potato, boil after 5min, by dry Cortex Cunninghamiae Lanceolatae, dry oak
Trees bits, wheat bran, potato are pulled out, and agar is added into the water of reactor, and manganese sulfate, glucose are put into after agar melts,
PH value is adjusted between 6-7, filtering produces mother culture media.
It is used as further scheme of the invention：Original seed, Cultivar culture medium manufacturing process it is as follows：Weigh weed tree sawdust, it is small
Stirred after granule corn core, wheat bran, Chinese fir wood chip, land plaster, manganese sulfate, mixing, according still further to material-water ratio 1:1.2 ratio
The continuation that adds water is stirred, and adjusts pH value to be produced between 6-7.
It is used as further scheme of the invention：The manufacturing process of pixie stool planting material is as follows：By weed tree sawdust, robur leaf, small
After granule corn core, wheat bran, Chinese fir wood chip, land plaster, fertile soil, manganese sulfate are weighed according to parts by weight, add water 120 parts, then
It is well mixed, fermented, when material temperature reaches 65 DEG C of holding 24h, then turning, every turning in 3 days once, continuous turning 3 times
Afterwards, open wide stockpile to be cooled, adjust moisture, adjustment pH is 6-7, when greenhouse cooling is produced to less than 30 DEG C in material.
It is used as further scheme of the invention：Mother culture media also includes sterilization steps after completing：By the mother of gained
Culture medium is planted, is put into vertically in high-pressure sterilizing pot, in 1.5kg/cm2Condition of high voltage under sterilizing 30min produce.
It is used as further scheme of the invention：Original seed, Cultivar culture medium also include sterilization steps after completing：By original
Kind, Cultivar culture medium, be placed in high-pressure sterilizing pot or normal-pressure sterilizing pot complete sterilizing, wherein in high-pressure sterilizing pot with
2kg/cm2Condition of high voltage under sterilize 180min, be after temperature reaches 100 DEG C, to keep 10h in normal-pressure sterilizing pot sterilising conditions.
A kind of method of energy large-scale planting pixie stool, comprises the following steps：
（1）Inoculation and culture
A, parent species inoculation and incubation step：Aseptically, by it is clean, without worm, disease-free fresh pixie stool fructification use 75%
Alcohol-pickled 1min, places into and 2min is soaked in sterilized water, then pulls pixie stool fructification out and is put on blotting paper, sucks water
Point, 0.5 × 0.5cm pixie stool fructification mushroom meat is cut between pixie stool fructification mushroom lid and mushroom handle, by pixie stool fructification
In Tube propagation base of the mushroom meat access containing mother culture media, culture produces pixie stool in 6 days in 23-25 DEG C of constant incubator
Parent species；
B, original seed inoculation and incubation step：Take pixie stool parent species to be put on pedigree seed culture medium, be placed on 23~25 DEG C of culturing room
Middle culture 30 days, produces pixie stool original seed；
C, cultigen inoculation and incubation step：Pixie stool original seed is taken to be put into Cultivar culture medium, in 23~25 DEG C of culturing room
Middle culture 30 days, produces pixie stool cultigen；
（2）Chicken fat bacteria cultivation
A, selection of land do furrow and stone is sowed：Select irrigation and drainage it is good, around pollution-free source, loosing soil, not hardened paddy field, drought it is flat
Ground or forest land, make the wide 55-65cm of furrow, the long rectangle ridge-up bed not limited, and the distance stayed between furrow and furrow is 40-50 cm；
Ridge-up bed spreads one layer of pulverized limestone on ridge-up bed surface after carrying out, and then repaves material sowing；When stone is sowed, a thickness 6cm chicken fat is first spread
Bacteria cultivation material, broadcasts sowing one layer of pixie stool cultigen, repaves pixie stool planting material 8cm thickness, broadcasts sowing one layer of pixie stool cultigen, then
A thickness 3cm pixie stool planting materials are spread on pixie stool cultigen, then sprinkle in pixie stool planting material that 2cm is thick, water content
For 35% fertile soil, one layer of leaf or wheat straw heat and moisture preserving are covered after the completion of sowing again, mushroom bed is made；
B, management of producing mushroom：Growth conditions in after planting 30 days is：22-28 DEG C of temperature, the upper humidity 70% of mushroom bed；After planting 31-
The growth conditions of 45 days is：25 DEG C -31 DEG C of temperature, the upper humidity 95% of mushroom bed, intensity of illumination 1000-1100Lx；When pixie stool mycelia
By the growth of 45 days, the pixie stool mycelia in raw material grew and in fertile soil superficial growth, to strengthen management of producing mushroom substantially
Work, 28-31 DEG C of pixie stool sporophore growth preference temperature need to be adjusted, when temperature be less than 20 DEG C or not long more than 38 DEG C
Mushroom, pixie stool fructification is a little needed 5~10 days from showing money or valuables one carries unintentionally to maturation；
C, harvesting：Chicken fat mushroom fructification lid can be harvested when being not yet fully deployed.
Compared with prior art, the beneficial effects of the invention are as follows：
Wild pixie stool is carried out tissue separation by the present invention, cultivates parent species, then parent species are accessed into original seed, and original seed is linked into cultigen
Cultigen is trained in culture medium, then cultigen is seeded into pixie stool compost, pixie stool is produced, had both enriched rare chicken
The kind supply of oily bacterium market, protects the wild resource of the species, with higher economic and social benefit again；And work of the present invention
Skill is easy, pollution rate is low, strain is pure, simple to operate, and cultivation period is short, and biological efficiency reaches 70%, is worth batch production, industry
Change popularization and application.
Specific advantage is as described below
（1）During the culture medium for making pixie stool, with weed tree sawdust, robur leaf, little particle corncob, wheat bran, Chinese fir wood chip, stone
Cream powder, fertile soil are mixed into major ingredient.Wherein weed tree sawdust, robur leaf, corncob, Chinese fir wood chip, wheat bran are natural plants.The present invention
Weed tree sawdust, robur leaf, corncob, Chinese fir wood chip, wheat bran are used cooperatively, make to produce synergy between them, carbon-nitrogen ratio is fitted
Close chicken fat bacteria growing.
(2) China still has substantial amounts of weed tree sawdust, robur leaf, corncob, Chinese fir wood chip to be wasted as discarded object every year.This
Invention is using weed tree sawdust, robur leaf, corncob, Chinese fir wood chip cultivation pixie stool technology, with weed tree sawdust, robur leaf, corncob, China fir
Trees bits not only take full advantage of the by-product goods and materials enriched the most during agricultural and forestry production as pixie stool Main Cultivation culture medium
Source, also the cultivation matrix for pixie stool provide abundant raw material sources.
(3) present invention also adds manganese sulfate (MnSO in the medium4), manganese element has to pixie stool mycelial growth
Obvious facilitation, is improved in pixie stool fruiting body yield, addition manganese sulfate (MnSO4) after, the biological transformation ratio of pixie stool
Significantly improve, yield is also more stablized.
（4）The present invention uses pixie stool compost cultivation in raw material, and contrast cultivates pixie stool with clinker, saves the energy,
Reduce environmental pollution.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of method of energy large-scale planting pixie stool comprises the following steps：
First, culture medium is made：
（1）Mother culture media：Following raw material is added in per 1000ml water：Dry Cortex Cunninghamiae Lanceolatae 50g, dry robur wood chip 50g, wheat bran 20g,
Potato（Peel, cut into slices）200g, glucose 20g, manganese sulfate 0.1g, agar 20g；
Manufacturing process is as follows：Weigh the dry Cortex Cunninghamiae Lanceolatae of respective amount, dry robur wood chip, wheat bran, potato（Peel, cut into slices）, grape
Sugar, manganese sulfate (MnSO4), agar, water.First by dry Cortex Cunninghamiae Lanceolatae, dry robur wood chip, wheat bran gauze wrapped, stainless-steel pan is put into
In, add water 2000ml, keeps 10min after water is boiled, is then placed in potato, boils after 5min, by dry Cortex Cunninghamiae Lanceolatae, dry robur wood
Bits, wheat bran, potato are pulled out, and stainless-steel pan reclaimed water is poured into measuring cup rapidly and measured, and water is 1100ml, then will amount
Water in cup is poured into stainless-steel pan, places into agar, and after being put into manganese sulfate, glucose after agar thawing, then adjustment is cultivated
The pH value of base is between 6-7, finally with 5 layers of filtered through gauze culture medium, the mother culture media after filtering be dispensed into while hot test tube it
In, culture medium dispensed loading amount is no more than 1/3rd of test tube overall length, and tampon stoppered test tube mouthful is used in packing after finishing.
（2）Original seed, Cultivar culture medium, include according to the raw material of parts by weight：60 parts of weed tree sawdust, little particle corncob 15
Part, 15 parts of wheat bran, 8 parts of Chinese fir wood chip, 2 parts of land plaster, 0.05 part of manganese sulfate；
Manufacturing process is as follows：Weigh weed tree sawdust, little particle corncob, wheat bran, Chinese fir wood chip, the land plaster of respective amount, manganese sulfate,
Then manually or mixer stirs culture medium, the culture medium continuation that added water after stirring is stirred, and amount of water is material
Water compares 1:1.2, the pH value of culture medium is then adjusted, then pH value must bottle or pack between 6-7.
（3）（4）Medium sterilization：
A, mother culture media sterilization steps：By the Tube propagation base containing mother culture media of gained, high pressure is put into vertically
In autoclave, (1.5kg/cm under elevated pressure conditions2) sterilizing 30min, after the completion of sterilizing, when being cooled to test tube non-scald on hand
It is drawn off, test tube is then put into inclined-plane, treats that invisible spectro culture medium in vitro thoroughly solidifies, it is completely stand-by after cooling.
B, original seed, Cultivar culture medium sterilization steps：
Original seed, the Cultivar culture medium of bottle or polybag will be installed, be placed in high-pressure sterilizing pot or normal-pressure sterilizing pot,
(2kg/cm under condition of high voltage2) sterilizing 180min, under normal-pressure sterilizing pot sterilising conditions, temperature is reached after 100 DEG C, keeps 10h
Sterilizing can be completed.
（5）Inoculation and culture
A, parent species inoculation and incubation step：By the Tube propagation base containing mother culture media of complete cooled and solidified, it is placed on super
In net workbench or inoculating hood, aseptically, by it is clean, without worm, disease-free fresh 75% alcohol of pixie stool fructification
1min is soaked, places into and 2min is soaked in sterilized water, then pixie stool fructification is pulled out and is put on blotting paper, moisture is sucked,
0.5 × 0.5cm pixie stool fructification mushroom meat is finally cut between pixie stool fructification mushroom lid and mushroom handle with scalpel, examination is taken out
Pipe tampon, is accessed 0.5 × 0.5cm pixie stool fructification mushroom meat in the Tube propagation base containing mother culture media with transfer needle
Between, tampon stoppered test tube mouthful is used in inoculation after finishing, be then placed in constant incubator, is cultivated 6 days under 23-25 DEG C of temperature conditionss
.
B, original seed inoculation and incubation step：Sterilized pedigree seed culture medium is put into aseptic inoculation box, original seed training is opened
Base tampon or bottle cap are supported, with transfer needle on alcolhol burner flame, a fritter is chosen from test tube stock rapidly and carries culture medium
Pixie stool mycelium, is put into bottle or on the pedigree seed culture medium of polybag, then covers tampon bottle cap or bottle cap.After inoculation
Original seed is placed in culturing room and cultivated, and the temperature of culturing room is maintained between 23~25 DEG C, is cultivated 30 days, is treated that mycelia covers with full bottle
Afterwards, you can for connecing cultigen.
C, cultigen inoculation and incubation step：Sterilized Cultivar culture medium is put into aseptic inoculation box, cotton is opened
Plug or bottle cap, are burnt with inoculation spoon on alcolhol burner flame, inoculation spoon after burning it is rapid chosen from original seed one piece 2.5 ×
2cm pixie stool mycelium original seed, is put into rapidly in cultigen bottle or on plastics bag culture medium, then covers tampon or bottle
Lid.Cultigen after inoculation is placed in culturing room and cultivated, and the temperature of culturing room is maintained between 23~25 DEG C, is cultivated 30 days, is treated
Mycelia is covered with after full bottle or full bag, you can for cultivating.
2nd, pixie stool cultural method：
A, pixie stool planting material are prepared：50 parts of weed tree sawdust, 10 parts of robur leaf, 15 parts of little particle corncob, 15 parts of wheat bran, Chinese fir wood
8 parts of bits, 2 parts of land plaster, 5 parts of fertile soil, 0.05 part of manganese sulfate, described pixie stool planting material are prepared：By above parts by weight
Raw material add water 120 parts, be then well mixed, pile wide 2 meters, high 1.5 meters of heap is fermented, when material temperature reaches 65 DEG C of guarantors
24h is held, then turning, every turning in 3 days once, continuous turning 3 times, you can open wide stockpile and cooled, adjust moisture, adjust
PH is 6-7, when greenhouse cooling, to less than 30 DEG C, carries out stone sowing in material.
B, selection of land do furrow and stone is sowed：Select irrigation and drainage good, around pollution-free source, loosing soil is not hardened, and traffic is just
The paddy field of profit, non-irrigated level land or forest land, make the wide 60cm of furrow, and the long rectangle ridge-up bed not limited stays pavement between furrow and furrow
40-50 cm.Furrow spread one layer of pulverized limestone in furrow face after carrying out, then repave material sowing, when stone is sowed, first spread a thickness 6cm
Pixie stool planting material, broadcasts sowing one layer of pixie stool cultigen, repaves material 8cm thickness, one layer of pixie stool cultigen is broadcasted sowing, then in cultivation
One thickness 3cm pixie stool planting materials of paving above kind, then it is 35% fertile soil to sprinkle the thick water content of 2cm in pixie stool planting material, often
Square metre application rate is 1 kilogram, and gently one layer of leaf or wheat straw heat and moisture preserving are covered on mushroom bed again after the completion of sowing.
C, management of producing mushroom：In after planting 30 days, 22-28 DEG C of temperature, the upper humidity 70% of mushroom bed, after planting the 31-45 days, temperature
25 DEG C -31 DEG C of degree, the upper humidity 95% of mushroom bed, intensity of illumination 1000-1100Lx.When pixie stool mycelia passes through the growth of 45 days or so,
Mycelia in raw material grows and in fertile soil superficial growth, to strengthen the work of management of producing mushroom substantially.Mainly moisture, temperature
Management, fertile soil surface can not be too dry, and chicken fat mushroom grows directly from seeds long 28-31 DEG C of optimal temperature, when temperature is less than 20 DEG C or exceedes
38 DEG C not long mushroom, fructification is a little needed 5~10 days from showing money or valuables one carries unintentionally to maturation.
D, harvesting：Chicken fat mushroom fructification lid can be harvested when being not yet fully deployed.With thumb, forefinger and middle finger during harvesting
Stem base portion is gripped, another hand pressure matrix, gently reverses and up pulls up, be careful not to loosen the young mushroom in edge, made with exerting oneself
Cheng Yougu is dead.Base portion cave is left on mushroom bed to be filled up with soil, remove the residual mushroom stayed on mushroom bed, pressed after harvesting after harvesting
Mushroom management method of breaking forth is managed, and cultivates mushroom next time, and whole growth period can receive 3 damp mushrooms, alternate 10~13 days per damp mushroom.
Embodiment 2
In the embodiment of the present invention, a kind of method of energy large-scale planting pixie stool, specific preparation process is same as Example 1, no
Be：Mother culture media：Following raw material is added in per 2000ml water：Dry Cortex Cunninghamiae Lanceolatae 45g, dry robur wood chip 45g, wheat bran 15g,
Potato 180g, glucose 15g, manganese sulfate 0.05g, agar 15g.Original seed, Cultivar culture medium, according to the raw material bag of parts by weight
Include：55 parts of weed tree sawdust, 10 parts of little particle corncob, 10 parts of wheat bran, 5 parts of Chinese fir wood chip, 1 part of land plaster, 0.02 part of manganese sulfate.Chicken
Oily bacteria cultivation material, includes according to the raw material of parts by weight：45 parts of weed tree sawdust, 5 parts of robur leaf, 10 parts of little particle corncob, wheat bran 10
Part, 5 parts of Chinese fir wood chip, 1 part of land plaster, 3 parts of fertile soil, 0.02 part of manganese sulfate.
Embodiment 3
In the embodiment of the present invention, a kind of method of energy large-scale planting pixie stool, specific preparation process is same as Example 1, no
Be：Mother culture media：Following raw material is added in per 2000ml water：Dry Cortex Cunninghamiae Lanceolatae 55g, dry robur wood chip 55g, wheat bran 25g,
Potato 220g, glucose 25g, manganese sulfate 0.15g, agar 25g.Original seed, Cultivar culture medium, according to the raw material bag of parts by weight
Include：65 parts of weed tree sawdust, 20 parts of little particle corncob, 20 parts of wheat bran, 10 parts of Chinese fir wood chip, 3 parts of land plaster, 0.1 part of manganese sulfate.Chicken
Oily bacteria cultivation material, includes according to the raw material of parts by weight：55 parts of weed tree sawdust, 15 parts of robur leaf, 20 parts of little particle corncob, wheat bran 20
Part, 10 parts of Chinese fir wood chip, 3 parts of land plaster, 7 parts of fertile soil, 0.1 part of manganese sulfate.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (10)
1. a kind of culture medium of energy large-scale planting pixie stool, including mother culture media, original seed, Cultivar culture medium and pixie stool
Planting material；Characterized in that, wherein mother culture media：Following raw material is added in per 2000ml water：Dry Cortex Cunninghamiae Lanceolatae 45-55g, dry oak
Trees bits 45-55g, wheat bran 15-25g, potato 180-220g, glucose 15-25g, manganese sulfate 0.05-0.15g, agar 15-
25g；
Original seed, Cultivar culture medium, include according to the raw material of parts by weight：55-65 parts of weed tree sawdust, 10-20 parts of little particle corncob,
10-20 parts of wheat bran, 5-10 parts of Chinese fir wood chip, 1-3 parts of land plaster, 0.02-0.1 parts of manganese sulfate；
Pixie stool planting material, includes according to the raw material of parts by weight：45-55 parts of weed tree sawdust, 5-15 parts of robur leaf, little particle corncob
10-20 parts, 10-20 parts of wheat bran, 5-10 parts of Chinese fir wood chip, 1-3 parts of land plaster, 3-7 parts of fertile soil, 0.02-0.1 parts of manganese sulfate.
2. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that mother culture media：Often
Following raw material is added in 2000ml water：Dry Cortex Cunninghamiae Lanceolatae 50g, dry robur wood chip 50g, wheat bran 20g, potato 200g, glucose
20g, manganese sulfate 0.1g, agar 20g.
3. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that original seed, cultigen training
Base is supported, is included according to the raw material of parts by weight：60 parts of weed tree sawdust, 15 parts of little particle corncob, 15 parts of wheat bran, 8 parts of Chinese fir wood chip, stone
2 parts of cream powder, 0.05 part of manganese sulfate.
4. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that pixie stool planting material,
Include according to the raw material of parts by weight：50 parts of weed tree sawdust, 10 parts of robur leaf, 15 parts of little particle corncob, 15 parts of wheat bran, Chinese fir wood chip
8 parts, 2 parts of land plaster, 5 parts of fertile soil, 0.05 part of manganese sulfate.
5. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that the system of mother culture media
Make process as follows：Dry Cortex Cunninghamiae Lanceolatae, dry robur wood chip, wheat bran, potato, glucose, manganese sulfate, agar, water are weighed, first by dry China fir
Bark, dry robur wood chip, wheat bran gauze wrapped, insert in reactor, add water, and keep 10min after water is boiled, are then placed in horse
Bell potato, boils after 5min, and dry Cortex Cunninghamiae Lanceolatae, dry robur wood chip, wheat bran, potato are pulled out, agar is added into the water of reactor,
After being put into manganese sulfate, glucose after agar thawing, adjustment pH value is between 6-7, and filtering produces mother culture media.
6. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that original seed, cultigen training
The manufacturing process for supporting base is as follows：Weed tree sawdust, little particle corncob, wheat bran, Chinese fir wood chip, land plaster, manganese sulfate are weighed, after mixing
Stir, according still further to material-water ratio 1:1.2 ratio, which adds water, to be continued to stir, and adjusts pH value to be produced between 6-7.
7. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that pixie stool planting material
Manufacturing process is as follows：By weed tree sawdust, robur leaf, little particle corncob, wheat bran, Chinese fir wood chip, land plaster, fertile soil, manganese sulfate
After being weighed according to parts by weight, add water 120 parts, be then well mixed, fermented, when material temperature reaches 65 DEG C of holding 24h, then turn over
Heap, every turning in 3 days once, after continuous turning 3 times, opens wide stockpile and is cooled, adjust moisture, adjustment pH is 6-7, when in material
Greenhouse cooling is produced to less than 30 DEG C.
8. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that Mother culture matrix manufacturing
After the completion of also include sterilization steps：It is put into high-pressure sterilizing pot, the mother culture media of gained in 1.5kg/cm vertically2
Condition of high voltage under sterilizing 30min produce.
9. the culture medium of energy large-scale planting pixie stool according to claim 1, it is characterised in that original seed, cultigen training
Also include sterilization steps after the completion of foster matrix manufacturing：By original seed, Cultivar culture medium, high-pressure sterilizing pot or normal-pressure sterilization are placed on
Sterilizing is completed in pot, wherein with 2kg/cm in high-pressure sterilizing pot2Condition of high voltage under sterilize 180min, gone out in normal-pressure sterilizing pot
Bacterium condition is that temperature is reached after 100 DEG C, keeps 10h.
10. a kind of culture medium using as described in claim 1-9 is any carries out the method for large-scale planting pixie stool, its feature
It is, comprises the following steps：
（1）Inoculation and culture
A, parent species inoculation and incubation step：Aseptically, by it is clean, without worm, disease-free fresh pixie stool fructification use 75%
Alcohol-pickled 1min, places into and 2min is soaked in sterilized water, then pulls pixie stool fructification out and is put on blotting paper, sucks water
Point, 0.5 × 0.5cm pixie stool fructification mushroom meat is cut between pixie stool fructification mushroom lid and mushroom handle, by pixie stool fructification
In Tube propagation base of the mushroom meat access containing mother culture media, culture produces pixie stool in 6 days in 23-25 DEG C of constant incubator
Parent species；
B, original seed inoculation and incubation step：Take pixie stool parent species to be put on pedigree seed culture medium, be placed on 23~25 DEG C of culturing room
Middle culture 30 days, produces pixie stool original seed；
C, cultigen inoculation and incubation step：Pixie stool original seed is taken to be put into Cultivar culture medium, in 23~25 DEG C of culturing room
Middle culture 30 days, produces pixie stool cultigen；
（2）Chicken fat bacteria cultivation
A, selection of land do furrow and stone is sowed：Select irrigation and drainage it is good, around pollution-free source, loosing soil, not hardened paddy field, drought it is flat
Ground or forest land, make the wide 55-65cm of furrow, the long rectangle ridge-up bed not limited, and the distance stayed between furrow and furrow is 40-50 cm；
Ridge-up bed spreads one layer of pulverized limestone on ridge-up bed surface after carrying out, and then repaves material sowing；When stone is sowed, a thickness 6cm chicken fat is first spread
Bacteria cultivation material, broadcasts sowing one layer of pixie stool cultigen, repaves pixie stool planting material 8cm thickness, broadcasts sowing one layer of pixie stool cultigen, then
A thickness 3cm pixie stool planting materials are spread on pixie stool cultigen, then sprinkle in pixie stool planting material that 2cm is thick, water content
For 35% fertile soil, one layer of leaf or wheat straw heat and moisture preserving are covered after the completion of sowing again, mushroom bed is made；
B, management of producing mushroom：Growth conditions in after planting 30 days is：22-28 DEG C of temperature, the upper humidity 70% of mushroom bed；After planting 31-
The growth conditions of 45 days is：25 DEG C -31 DEG C of temperature, the upper humidity 95% of mushroom bed, intensity of illumination 1000-1100Lx；When pixie stool mycelia
By the growth of 45 days, the pixie stool mycelia in raw material grew and in fertile soil superficial growth, to strengthen management of producing mushroom substantially
Work, 28-31 DEG C of pixie stool sporophore growth preference temperature need to be adjusted, when temperature be less than 20 DEG C or not long more than 38 DEG C
Mushroom, pixie stool fructification is a little needed 5~10 days from showing money or valuables one carries unintentionally to maturation；
C, harvesting：Chicken fat mushroom fructification lid can be harvested when being not yet fully deployed.
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