CN107228885B - 一种色素纳米囊泡仿生气体传感器的制备方法 - Google Patents
一种色素纳米囊泡仿生气体传感器的制备方法 Download PDFInfo
- Publication number
- CN107228885B CN107228885B CN201710511776.3A CN201710511776A CN107228885B CN 107228885 B CN107228885 B CN 107228885B CN 201710511776 A CN201710511776 A CN 201710511776A CN 107228885 B CN107228885 B CN 107228885B
- Authority
- CN
- China
- Prior art keywords
- pigment
- vesicle
- sensor
- nano
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000049 pigment Substances 0.000 title claims abstract description 61
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002502 liposome Substances 0.000 claims abstract description 7
- 239000002041 carbon nanotube Substances 0.000 claims abstract description 6
- 229910021393 carbon nanotube Inorganic materials 0.000 claims abstract description 6
- 238000009826 distribution Methods 0.000 claims abstract description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000010931 gold Substances 0.000 claims abstract description 5
- 229910052737 gold Inorganic materials 0.000 claims abstract description 5
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 5
- 150000003904 phospholipids Chemical class 0.000 claims abstract description 5
- YIYFFLYGSHJWFF-UHFFFAOYSA-N [Zn].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 Chemical compound [Zn].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 YIYFFLYGSHJWFF-UHFFFAOYSA-N 0.000 claims description 6
- 150000004032 porphyrins Chemical class 0.000 claims description 5
- 238000003786 synthesis reaction Methods 0.000 claims description 4
- 238000002371 ultraviolet--visible spectrum Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 3
- 230000003592 biomimetic effect Effects 0.000 claims 3
- NVJHHSJKESILSZ-UHFFFAOYSA-N [Co].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 Chemical compound [Co].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 NVJHHSJKESILSZ-UHFFFAOYSA-N 0.000 claims 1
- NUSORQHHEXCNQC-UHFFFAOYSA-N [Cu].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 Chemical compound [Cu].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 NUSORQHHEXCNQC-UHFFFAOYSA-N 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 102000012547 Olfactory receptors Human genes 0.000 abstract description 2
- 108050002069 Olfactory receptors Proteins 0.000 abstract description 2
- 229910021432 inorganic complex Inorganic materials 0.000 abstract description 2
- 210000003370 receptor cell Anatomy 0.000 abstract description 2
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 10
- KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229950003776 protoporphyrin Drugs 0.000 description 3
- 239000012620 biological material Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229920001940 conductive polymer Polymers 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010897 surface acoustic wave method Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
本发明公开了一种色素纳米囊泡仿生气体传感器的制备方法,包括:步骤1:色素研制的步骤:首先通过气味分子与色素反应的结合能、偶极矩、中心金属离子偏离色素分子平面的距离变化情况,以及反应过程中轨道能级差、电荷分布变化信息,从理论上设计特异性和稳定性好的色素;步骤2:色素囊泡研制的步骤:通过色素与磷脂自组装成纳米囊泡脂质体;步骤3:传感器研制的步骤:利用碳纳米管作为介质将色素纳米囊泡固定在金叉指电极上,从而制成传感器。与传统型传感器相比,纳米囊泡仿生程度高;与细胞、蛋白等生物传感器相比,此类传感器为有机‑无机复合体,稳定性高。因此本发明用囊泡来模拟人嗅觉受体细胞研制新型仿生气体传感器。
Description
技术领域
本发明涉及一种气体传感器的发明,特指一种色素纳米囊泡仿生气体传感器的制备方法。
背景技术
目前,气体传感器分为传统型和生物型两类。传统型的气体传感器主要有金属氧化物型半导体传感器、有机导电聚合物传感器、质量传感器(包括石英晶体谐振传感器和声表面波传感器)、场效应管传感器、红外线光电传感器和金属栅MOS气体传感器等。生物型气体传感器主要是将细胞、蛋白、生物肽等固定在纳米导电基底(如石墨烯、碳纳米管、石英晶振片等)上来获得。
传统型气体传感器大都是基于物理吸附等分子间弱作用力,存在灵敏度一般低于人类相应感官的灵敏度、得到气味的整体信息与人类感官之间差距较大等缺点,有些传感器还存在工作温度高、反应时间长、设备体积大、温湿度干扰严重等缺点。而生物型主要存在容易失活、失性,稳定性低等缺点。另外,由于其研究门槛相对较高,生物材料生产费用高且不容易与基底材料结合,导致此类装置大多仍处于实验研究阶段。
囊泡是由两亲性分子自组装形成的一种超分子聚集体,其结构为密闭双分子层包裹形成的球形单腔室或多腔室结构,已在药物载体和靶向施药方面取得成功。但在气体传感器研制方面未见报道。
发明内容
本发明的色素纳米囊泡仿生气体传感器包括色素研制、色素囊泡研制及传感器研制三个部分。实现本发明的技术方案如下:
(1)色素研制。首先通过气味分子与色素反应的结合能、偶极矩、中心金属离子偏离色素分子平面的距离变化情况,以及反应过程中轨道能级差、电荷分布变化等信息,从理论上设计特异性和稳定性好的色素。并通过化学合成等方法制备相关色素,同时通过紫外-可见光谱反应与动力参数计算相结合的方式对色素与气味分子反应进行表征,确定所得的色素具有很好的分子识别性能。
(2)色素囊泡研制。通过色素与磷脂自组装成纳米囊泡脂质体,该纳米囊泡直径为几个纳米到几百个纳米,色素结合在囊泡的表面,具有极高的色素密度(>80000每颗粒)。
(3)传感器研制。利用碳纳米管作为介质将色素纳米囊泡固定在金叉指电极上制成传感器。
本发明的有益效果是:
囊泡特有的双层膜包裹亲水核的结构,与细胞的结构十分类似,使得其在模拟生物细胞方面发挥了较为重要的作用。与传统型传感器相比,纳米囊泡仿生程度高;与细胞、蛋白等生物传感器相比,此类传感器为有机-无机复合体,稳定性高。因此本发明用囊泡来模拟人嗅觉受体细胞研制新型仿生气体传感器。
附图说明
图1:色素纳米囊泡的结构图;
图2:传感器研制示意图;
图3:传感器与三甲胺反应情况示意图;
具体实施方式
下面结合附图和具体实施例对本发明作进一步说明。
首先通过气味分子与色素反应的结合能、偶极矩、中心金属离子偏离色素分子平面的距离变化情况,以及反应过程中轨道能级差、电荷分布变化等信息,从理论上设计特异性和稳定性好的色素。并通过化学合成等方法制备相关色素。最后通过紫外-可见光谱反应与动力参数计算相结合的方式对色素与气味分子反应进行表征,确定所得的色素具有很好的分子识别性能。
其次通过色素与磷脂自组装成纳米囊泡脂质体,该纳米囊泡直径为几个纳米到几百个纳米,色素结合在囊泡的表面,具有极高的色素密度(>80000每颗粒)。
利用碳纳米管作为介质将色素纳米囊泡固定在金叉指电极上制成传感器。
卟啉类色素纳米囊泡传感器实例:
卟啉类色素是一组比较特殊的色素化合物,以原卟啉为模板,以三甲胺为检测对象,本发明的实施实例如下。
通过改变原卟啉中心离子和***碳链,计算其与三甲胺接触时的结合能、偶极矩、中心金属离子偏离色素分子平面的距离变化情况,以及反应过程中轨道能级差、电荷分布变化等信息,锌卟啉的变化最大,并通过化学合成制备锌卟啉,同时通过紫外-可见光谱反应与动力参数计算相结合的方式确定锌卟啉有很好的三甲胺分子识别能力。
然后让锌卟啉,与磷脂自组装成锌卟啉纳米囊泡脂质体如图1所示。经电镜表征,该囊泡直径为100纳米左右。
最后用碳纳米管作为介质将卟啉色素纳米囊泡固定在金叉指电极上制成传感器如图2所示。
研制的传感器与三甲胺反应曲线如图3所示,该传感器具有很好的重复性和稳定性。
上文所列出的一系列的详细说明仅仅是针对本发明的可行性实施方式的具体说明,它们并非用以限制本发明的保护范围,凡未脱离本发明技艺精神所作的等效实施方式或变更均应包含在本发明的保护范围之内。
Claims (4)
1.一种色素纳米囊泡仿生气体传感器的制备方法,其特征在于,包括:
步骤1:色素研制的步骤:首先通过气味分子与色素反应的结合能、偶极矩、中心金属离子偏离色素分子平面的距离变化情况,以及反应过程中轨道能级差、电荷分布变化信息,从理论上设计特异性和稳定性好的色素;
步骤2:色素囊泡研制的步骤:通过色素与磷脂自组装成纳米囊泡脂质体;所述纳米囊泡直径为几个纳米到几百个纳米;所述纳米囊泡的色素结合在囊泡的表面;所述纳米囊泡具有极高的色素密度,其色素密度为>80000每颗粒;
步骤3:传感器研制的步骤:利用碳纳米管作为介质将色素纳米囊泡固定在金叉指电极上,从而制成传感器。
2.根据权利要求1所述的一种色素纳米囊泡仿生气体传感器的制备方法,其特征在于,步骤1还包括:通过化学合成方法制备相关色素,同时通过紫外-可见光谱反应与动力参数计算相结合的方式对色素与气味分子反应进行表征,确定所得的色素具有很好的分子识别性能。
3.根据权利要求1-2任一项 所述的一种色素纳米囊泡仿生气体传感器的制备方法,其特征在于,所述方法应用于卟啉类色素纳米囊泡传感器的制备时,色素包含锌卟啉,铜卟啉,钴卟啉。
4.根据权利要求1-2任一项 所述的一种色素纳米囊泡仿生气体传感器的制备方法,其特征在于,所述方法应用于卟啉类色素纳米囊泡传感器的制备时,囊泡直径为100纳米左右。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710511776.3A CN107228885B (zh) | 2017-06-29 | 2017-06-29 | 一种色素纳米囊泡仿生气体传感器的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710511776.3A CN107228885B (zh) | 2017-06-29 | 2017-06-29 | 一种色素纳米囊泡仿生气体传感器的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107228885A CN107228885A (zh) | 2017-10-03 |
CN107228885B true CN107228885B (zh) | 2020-06-26 |
Family
ID=59935242
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710511776.3A Expired - Fee Related CN107228885B (zh) | 2017-06-29 | 2017-06-29 | 一种色素纳米囊泡仿生气体传感器的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107228885B (zh) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6939721B2 (en) * | 2000-12-18 | 2005-09-06 | Agilent Technologies, Inc. | Fluorescence immunoassays using organo-metallic complexes for energy transfer |
WO2003046536A1 (en) * | 2001-11-26 | 2003-06-05 | Sony International (Europe) Gmbh | The use of 1d semiconductor materials as chemical sensing materials, produced and operated close to room temperature |
CN100392385C (zh) * | 2002-12-03 | 2008-06-04 | 博奥生物有限公司 | 亲和反应的化学放大电化学检测方法及其试剂盒 |
JP4966435B2 (ja) * | 2010-08-05 | 2012-07-04 | パナソニック株式会社 | ガス分子検知素子、ガス分子検知装置及びガス分子検知方法 |
CN203216929U (zh) * | 2013-02-26 | 2013-09-25 | 裴振华 | 基于导电聚合物的牙周细菌检测生物芯片 |
CN103575771B (zh) * | 2013-11-20 | 2015-07-29 | 江苏大学 | 一种气体传感器及其制作方法 |
CN106415255B (zh) * | 2014-03-02 | 2020-08-07 | 麻省理工学院 | 基于金属碳配合物的气体感测器 |
-
2017
- 2017-06-29 CN CN201710511776.3A patent/CN107228885B/zh not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN107228885A (zh) | 2017-10-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Wang et al. | Direct electrochemical DNA biosensor based on reduced graphene oxide and metalloporphyrin nanocomposite | |
Wang et al. | Graphene‐functionalized natural microcapsules: modular building blocks for ultrahigh sensitivity bioelectronic platforms | |
Pandey et al. | Highly sensitive electrochemical immunosensor based on graphene-wrapped copper oxide-cysteine hierarchical structure for detection of pathogenic bacteria | |
Qin et al. | A novel signal amplification strategy of an electrochemical aptasensor for kanamycin, based on thionine functionalized graphene and hierarchical nanoporous PtCu | |
Wang et al. | Electrochemical biosensors based on antibody, nucleic acid and enzyme functionalized graphene for the detection of disease-related biomolecules | |
He et al. | A multi-walled carbon nanotubes-poly (L-lysine) modified enantioselective immunosensor for ofloxacin by using multi-enzyme-labeled gold nanoflower as signal enhancer | |
Weng et al. | A unique turn-off fluorescent strategy for sensing dopamine based on formed polydopamine (pDA) using graphene quantum dots (GQDs) as fluorescent probe | |
Zhu et al. | Magnetically controlled electrochemical sensing membrane based on multifunctional molecularly imprinted polymers for detection of insulin | |
Jeyapragasam et al. | Electrochemical biosensing of carbofuran based on acetylcholinesterase immobilized onto iron oxide–chitosan nanocomposite | |
Qiu et al. | A label-free amperometric immunosensor based on biocompatible conductive redox chitosan-ferrocene/gold nanoparticles matrix | |
Guo et al. | RGD-peptide functionalized graphene biomimetic live-cell sensor for real-time detection of nitric oxide molecules | |
Han et al. | Highly conducting gold nanoparticles–graphene nanohybrid films for ultrasensitive detection of carcinoembryonic antigen | |
Zhu et al. | Urchinlike MnO2 nanoparticles for the direct electrochemistry of hemoglobin with carbon ionic liquid electrode | |
Chauhan et al. | Label-free piezoelectric immunosensor decorated with gold nanoparticles: Kinetic analysis and biosensing application | |
Elancheziyan et al. | Covalent immobilization and enhanced electrical wiring of hemoglobin using gold nanoparticles encapsulated PAMAM dendrimer for electrochemical sensing of hydrogen peroxide | |
Che et al. | Amperometric immunosensor for the determination of α-1-fetoprotein based on multiwalled carbon nanotube–silver nanoparticle composite | |
Ge et al. | based biosensor relying on flower-like reduced graphene guided enzymatically deposition of polyaniline for Pb2+ detection | |
Guo et al. | The application of thionine–graphene nanocomposite in chiral sensing for tryptophan enantiomers | |
KR20130128220A (ko) | 환원된 그래핀 산화물 층을 포함하는 바이오 센서 | |
Li et al. | A sensitive electrochemical immunosensor for prion detection based on poly-β-cyclodextrin/gold nanoparticles/glassy carbon electrode | |
CN111122679B (zh) | 一种dna生物传感器及其制备方法和应用 | |
Yang et al. | Portable and on-site electrochemical sensor based on surface molecularly imprinted magnetic covalent organic framework for the rapid detection of tetracycline in food | |
CN109164149B (zh) | 一种基于纸芯片结合丝网印刷电极在线检测细胞内过氧化氢的电化学生物传感器 | |
Ding et al. | Triggering interface potential barrier: A controllable tuning mechanism for electrochemical detection | |
Li et al. | An amperometric immunosensor with a DNA polyion complex membrane/gold nanoparticles-backbone for antibody immobilisation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200626 |