CN107216998A - The automation equipment of bacterial content in rapid measurement oilfield sewage and product oil - Google Patents
The automation equipment of bacterial content in rapid measurement oilfield sewage and product oil Download PDFInfo
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- CN107216998A CN107216998A CN201610163852.1A CN201610163852A CN107216998A CN 107216998 A CN107216998 A CN 107216998A CN 201610163852 A CN201610163852 A CN 201610163852A CN 107216998 A CN107216998 A CN 107216998A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
The present invention relates to the automation equipment of bacterial content in a kind of rapid measurement oilfield sewage and product oil, the problem of measuring speed is relatively slow, not convenient, result is inaccurate in the prior art is mainly solved.The present invention is by using a kind of rapid automation equipment for measuring bacterial content in oilfield sewage and product oil, including casing, Enrichment of bacteria and nucleic acid molecules extraction equipment, the technical scheme that Enrichment of bacteria and the nucleic acid molecules extraction equipment includes Reagent Tube placement location (1) and Reagent Tube placement location (4), filter membrane (2), adsorption layer (3) reaction tank (5), light source (6), optical filter (7), photoreceptor (8) and at least four valves preferably solves above mentioned problem, available for measuring in fluid sample in bacterial content.
Description
Technical field
The present invention relates to the automation equipment of bacterial content in a kind of rapid measurement oilfield sewage and product oil.
Background technology
The corrosion failure of material as caused by the vital movement of the microorganisms such as bacterium is referred to as microbiologic(al) corrosion (MIC).MIC
It is a kind of phenomenon of generally existing.All existed in oil gas field, oil tank, gas station, oil circuit even automotive oil tank substantial amounts of thin
Bacterium.It is annual because corrosion and caused by economic loss more than 10,000,000,000 dollars.Wherein topmost corrosion-causing bacteria is sulfate
Reducing bacteria (SRB), causes the corrosion loss of oil well more than 77% produced in USA.SRB can under oxygen-free environment, with
Organic matter in environment is carbon source, is hydrogen sulfide by sulfate reduction, from oxidation also using the hydrogen produced in bacterial biof iotalm
Energy is obtained in original reaction, and metal is also oxidized corrosion in the process.Current SRB detection method mainly has three kinds,
Respectively：Cultivate detection technique, microscopic techniques and biochemical Direct Inspection Technology.Culture detection technique is current application
A kind of relatively broad method, its principle is that sample is done into gradient dilution, is cultivated on appropriate culture medium, final root
Quantified according to the positive reaction of extension rate and bacterium.The Main Basiss of the method are the disappearances that American Petroleum Institute API recommends
Dilution method, China's oil professional standard SY/T 0532-2012《Oilfield injection water bacteria analyzing method dilution-to-extinction method》In
There is specified in more detail.Patents have been reported (such as CN102329851A, CN103983636A, CN1769472A,
CN200510010459 etc.), there is also SRB special tests bottle is available for purchase in the market.Cultivate the excellent of detection technique
Point is simple to operate, with low cost, and as a result accuracy is higher.Its major defect is that time-consuming (2-14 days), culture
Strain not exclusively causes quantitative result relatively low.Once there is fungus grown, it is necessary to reform experiment in negative control group, it is difficult to ensure that
Resampling result is consistent with original sample.
Microscopic techniques combine specific stain method and fluorescence method of counting, and fluorophor is optionally tagged to
SRB surface, is then counted under fluorescence microscope with quantitative.But such a method still needs Short-term Culture to improve mushroom
Concentration, cultivation cycle was at two days or so.Although relatively easy, the quantitative cost of its operation is low, the Gao Te of dye molecule is needed
The opposite sex, and the relevant information of bacterial activity can not be provided.
Biochemical Direct Inspection Technology contains a variety of concrete modes【Close sulfate in the sparkling oilfield sewages of refined Xia Yehaichunfan
Progress chemistry and the bioengineering 2,012 29 17 of reducing bacteria detection technique】, inside SRB or surface can be detected
Certain albumen with enzyme-linked immunosorbent assay ELISA method (as determined 5'-AMP sulfate reduction enzyme APS【Such as
GB2354072B、CN103983636A、EP272916A1】), the specific sequence of nucleic acid molecules of SRB can be detected
(such as polymerase chain reaction PCR method【CN105112543A、CN1769472A、CN200510010459】, it is glimmering
Light in situ hybridization FISH methods【Wang Ming justice beam dogface Zheng Ya duckweeds Zhao by Wei in blue or green the sulfate reduction dientification of bacteria and inspection
The progress JOURNAL OF MICROBIOLOGY 2,005 25 81 of survey method】), SRB metabolite (such as current potential can also be detected
Method or indicator method determine H2S)【Research-sulphion choosing of Li Wan justice Zhao from light sulfate reducing bacteria bacterium amount detection method
Select electrode method chemical sensor 1,991 11 64】.These detection method accuracys are high, specificity is high, detection cycle is short (several
Hour or so), but complex operation, typically can only be real by trained professional's progress in special biochemical test room
Test.APS detection methods therein are relatively easy, and the kit of the existing commercialization of foreign countries is sold, it is possible to achieve rapid field is surveyed
Measure, but the shortcoming of this method has：1) while all bacterial species with APS of detection, as a result possible higher；2) tie
Fruit is inaccurate by index quantification of color change；3) bacteria lysis may not thoroughly cause to detect Lower result.
In summary, a kind of quick, convenient, accurate on-site measurement method and equipment are lacked at present.This patent is described
The specificity of bacterium, automation, portable quantitative testing device in a kind of sample based on nucleic acid molecules amplifying technique.
The content of the invention
The technical problems to be solved by the invention are the problem of measuring speed are relatively slow in the prior art, not convenient, result is inaccurate,
A kind of automation equipment of bacterial content in new rapid measurement oilfield sewage and product oil is provided.The device has quickly, just
Prompt, accurate advantage.
To solve the above problems, the technical solution adopted by the present invention is as follows：It is thin in a kind of rapid measurement oilfield sewage and product oil
The automation equipment of bacterial content, including casing, Enrichment of bacteria and nucleic acid molecules extraction equipment, the Enrichment of bacteria and nucleic acid point
Sub- extraction equipment includes Reagent Tube placement location (1) and Reagent Tube placement location (4), filter membrane (2), adsorption layer (3)
Reaction tank (5), light source (6), optical filter (7), photoreceptor (8) and at least four valves, the Reagent Tube are put
Seated position (1) bottom is connected with filter membrane (2) superjacent air space, and filter membrane (2) underlying space is divided into two-way, all the way with valve a
Arrival end is connected, and another road is connected with valve b arrival ends, and the valve a ports of export are connected with waste liquid pool, and the valve b ports of export lead to
Piping is connected with adsorption layer (3) entrance side, and the pipeline of adsorption layer (3) outlet side is divided into two-way, enters all the way with valve c
Mouth end is connected, and the valve c ports of export are connected with waste liquid pool, and another road is connected after valve d with reaction tank (5)；Reagent
Pipe placement location (4) bottom is connected with by the pipeline between pipeline and valve b and adsorption layer entrance side；Light source (6) is
Reaction tank (5) provides dye molecule excitation energy, and the fluorescence of generation is passed through after optical filter (7) by photoreceptor (8) reception；
The top, the bottom of Reagent Tube placement location (4) of filter membrane (2) superjacent air space are equipped with metal spine, when Reagent Tube is placed
The aluminium foil of reagent bottom of the tube can be punctured when on Reagent Tube placement location (1) or Reagent Tube placement location (4).
In above-mentioned technical proposal, it is preferable that when Reagent Tube is placed on Reagent Tube placement location (1), on Reagent Tube top
Piston is placed, the reagent in Reagent Tube is pressed downward in the space above filter membrane (2).
In above-mentioned technical proposal, it is preferable that the Enrichment of bacteria and accounting molecule catch integration of equipments in the casing.
In above-mentioned technical proposal, it is preferable that valve a~valve d is programme controlled magnetic valve, or using micro-fluidic micro-
Valve technology, by the switch of air pump control piper.
In above-mentioned technical proposal, it is preferable that the top of reaction tank (5) is opening or translucent material, internal preset reaction is female
Reagent ball after liquid or freeze-drying, nucleic acid eluents flow into reaction tank (5) and mixed afterwards with preset solution or reagent ball.
In above-mentioned technical proposal, it is preferable that the signal that photoreceptor (8) goes out is sent to signal processing unit and analyzed.
In above-mentioned technical proposal, it is preferable that Reagent Tube placement location (1) or (4) are placed in higher position, are easy under liquid
It flow to lower position.
In above-mentioned technical proposal, it is preferable that, it is necessary to set quick add on reaction tank (5) periphery when using PCR method
Hot-cold but module to realize the temperature cycles of denaturation-annealing-extension；When using isothermal amplification method, around reaction tank
Only need to set heating module.
In above-mentioned technical proposal, it is preferable that the replacing of Reagent Tube is completed by automatic control system.
In the present invention, Enrichment of bacteria is to flow through filter membrane by sample to realize.After sample is added, sample bottom of the tube Aluminium Foil Package
Dress is broken by lower metal spine, and liquid flows into the top of filter membrane 2, now up applies pressure, promotes liquid through filter membrane,
Solid matter more than filter sizes is left on filter membrane, liquid outflow.Now valve a is opened, and valve b is closed, waste liquid
Flow out to waste liquid pool.When sample is the aqueous phase substances such as oil field waste, using hydrophilic filter membrane, filter membrane size should be micro- 0.5
Rice is following, to ensure that objective microbe can be retained on filter membrane.When sample is the oil phase substances such as product oil, using oleophylic
Property filter membrane.After sample all flows through filter membrane, procedure auto-control sample cell substitutes to be preset at the ethanol solution in casing,
Above-mentioned pressurization flow is repeated to rinse filter membrane.Continue to substitute and be rinsed for deionized water or suitable pH buffer solution.Work as punching
Wash after end, add bacterial lysate, wait and start pressurization after certain time, now valve a is closed, valve b is opened,
Bacterial lysate enters nucleic acid extraction unit.DNA adsorption columns (in adsorption layer 3) are prefixed in nucleic acid extraction unit, on
The bacterial lysate flowed into is swum when flowing through adsorption layer 3, DNA therein is attracted on adsorption layer 3, liquid phase flows into useless
Liquid pool.Now valve c is opened, and valve d is closed.It is preset cleaning solution in test tube on Reagent Tube placement location 4, equally
Make it flow through adsorption layer 3 to take away impurity by pressure.The test tube on Reagent Tube placement location 4 is changed with repeated washing flow.
At the end of washing, it is the suitable buffer solutions of a small amount of pH to change solution in the test tube on Reagent Tube placement location 4.Now valve
Door c is closed, and valve d is opened.Buffer solution elutes DNA when flowing through adsorption layer 3, rinses to reaction tank 5.Instead
Each composition that nucleic acid iodine needs are prefixed in pond 5 is answered, can be the mother liquor prepared, or long-term guarantor
Deposit purpose and place the reagent after freeze-drying, by the buffer solution poured.Amplification and detection unit are by reaction tank 5, light source
(halogen lamp or LED) 6, optical filter 7, photoreceptor (CCD or PMT) 8 are constituted.When nucleic acid iodine is in reaction tank
When occurring in 5, the dye molecule in the light provocative reaction pond 5 that light source 6 is sent, the fluorescence of generation is received by photoreceptor 8.
Thus the concentration of dye molecule and the concentration positive correlation of nucleic acid in solution molecule, can judge the initial concentration of nucleic acid molecules.
The present apparatus contains Enrichment of bacteria, nucleic acid molecules and extracted and amplification three units of reading, is a kind of integrated portable
Self-reacting device, can realize in-site measurement.Enrichment of bacteria unit realizes point of bacterium by the way of sample flows through filter membrane
From and enrichment, required time greatly shortens compared with cultivation.Nucleic acid molecules are extracted and amplification is read on microfluidic platform
Realize.Extract part and use silica adsorption column technology adsorption of DNA molecule, reagent point is promoted with pressure by programme-control
Adsorption column is not flowed through, pure nucleic acid molecules are finally given.In amplification sensing element, PCR side can be selectively used
Method or ring mediated isothermal amplification (LAMP) method.Compared with conventional PCR method, the specificity of LAMP method is higher, right
The tolerance of impurity is higher in reaction medium, and reaction efficiency is higher, and detection time is shorter (can foreshorten to 20 minutes), and
- annealing-temperature cycles extended need not be denatured, the requirement to temperature control module is reduced.All operations are removed by programme-control
Sample-adding is outer without manually operated, improves the collimation and convenience of test.
Brief description of the drawings
Fig. 1 is the schematic appearance of device of the present invention；
Fig. 2 is each modular structure schematic diagram inside device of the present invention；
In Fig. 1, Fig. 2,1 is Reagent Tube placement location；2 be filter membrane；3 be adsorption layer；4 be that Reagent Tube places position
Put；5 be reaction tank；6 be light source；7 be optical filter；8 be receptor；A~d is valve.
Below by embodiment, the invention will be further elaborated, but is not limited only to the present embodiment.
Embodiment
【Embodiment 1】
The automation equipment of bacterial content in a kind of rapid measurement oilfield sewage and product oil, as shown in Figure 1 and Figure 2.Including
Casing, Enrichment of bacteria and nucleic acid molecules extraction equipment, Enrichment of bacteria and the nucleic acid molecules extraction equipment are placed including Reagent Tube
Position (1) and Reagent Tube placement location (4), filter membrane (2), adsorption layer (3) reaction tank (5), light source (6),
Optical filter (7), photoreceptor (8) and at least four valves, Reagent Tube placement location (1) bottom and filter membrane (2)
Superjacent air space is connected, and filter membrane (2) underlying space is divided into two-way, is connected all the way with valve a arrival ends, another road and valve b
Arrival end is connected, and the valve a ports of export are connected with waste liquid pool, and the valve b ports of export pass through pipeline and adsorption layer (3) entrance side
Be connected, the pipeline of adsorption layer (3) outlet side is divided into two-way, is connected all the way with valve c arrival ends, the valve c ports of export and
Waste liquid pool is connected, and another road is connected after valve d with reaction tank (5)；Reagent Tube placement location (4) bottom is with passing through
Pipeline between pipeline and valve b and adsorption layer entrance side is connected；Light source (6) is that reaction tank (5) offer dye molecule swashs
Energy is sent out, the fluorescence of generation is passed through after optical filter (7) by photoreceptor (8) reception；The top of filter membrane (2) superjacent air space,
The bottom of Reagent Tube placement location (4) is equipped with metal spine, when Reagent Tube is placed on Reagent Tube placement location (1) or examination
The aluminium foil of reagent bottom of the tube can be punctured when on agent pipe placement location (4).
Device of the present invention is integrated in 0.5m*0.4m*0.4m metal shell, and user only needs to opening as needed
Panel loads about 1mL fluid samples.
In the present invention, Enrichment of bacteria is to flow through filter membrane by sample to realize.After sample is added, sample bottom of the tube Aluminium Foil Package
Dress is broken by lower metal spine, and liquid flows into the top of filter membrane 2, now up applies pressure, promotes liquid through filter membrane,
Solid matter more than filter sizes is left on filter membrane, liquid outflow.Now valve a is opened, and valve b is closed, waste liquid
Flow out to waste liquid pool.When sample is the aqueous phase substances such as oil field waste, using hydrophilic filter membrane, filter membrane size should be micro- 0.5
Rice is following, to ensure that objective microbe can be retained on filter membrane.When sample is the oil phase substances such as product oil, using oleophylic
Property filter membrane.After sample all flows through filter membrane, procedure auto-control sample cell substitutes to be preset at the ethanol solution in casing,
Above-mentioned pressurization flow is repeated to rinse filter membrane.Continue to substitute and be rinsed for deionized water or suitable pH buffer solution.Work as punching
Wash after end, add bacterial lysate, wait and start pressurization after certain time, now valve a is closed, valve b is opened,
Bacterial lysate enters nucleic acid extraction unit.DNA adsorption columns (in adsorption layer 3) are prefixed in nucleic acid extraction unit, on
The bacterial lysate flowed into is swum when flowing through adsorption layer 3, DNA therein is attracted on adsorption layer 3, liquid phase flows into useless
Liquid pool.Now valve c is opened, and valve d is closed.It is preset cleaning solution in test tube on Reagent Tube placement location 4, equally
Make it flow through adsorption layer 3 to take away impurity by pressure.The test tube on Reagent Tube placement location 4 is changed with repeated washing flow.
At the end of washing, it is the suitable buffer solutions of a small amount of pH to change solution in the test tube on Reagent Tube placement location 4.Now valve
Door c is closed, and valve d is opened.Buffer solution elutes DNA when flowing through adsorption layer 3, rinses to reaction tank 5.Instead
Each composition that nucleic acid iodine needs are prefixed in pond 5 is answered, can be the mother liquor prepared, or long-term guarantor
Deposit purpose and place the reagent after freeze-drying, by the buffer solution poured.Amplification and detection unit are by reaction tank 5, light source
(halogen lamp or LED) 6, optical filter 7, photoreceptor (CCD or PMT) 8 are constituted.When nucleic acid iodine is in reaction tank
When occurring in 5, the dye molecule in the light provocative reaction pond 5 that light source 6 is sent, the fluorescence of generation is received by photoreceptor 8.
Thus the concentration of dye molecule and the concentration positive correlation of nucleic acid in solution molecule, can judge the initial concentration of nucleic acid molecules.
In order that advantages of the present invention, technical scheme are clearer, clear and definite, with reference to step of the specific embodiment to the present invention
Suddenly elaborate.
Liquid in a device flow through pressure-driven realization, wherein Reagent Tube placement location 1, Reagent Tube placement location 4
Higher position is placed in, is easy to liquid downstream to lower position, top rubber piston produces thrust during moving down, made
Liquid flow filter membrane and adsorption column.The bottom position of Reagent Tube placement location 1 and Reagent Tube placement location 4 is aluminium foil material,
The metal needle point that can be fixed when entering specified location by lower section is punctured, and makes the liquid outflow of the inside.Reagent Tube placement location 1
The preset solution in position is alcohol flushing liquid, wash buffer liquid and cell pyrolysis liquid (lysozyme, CTAB or SDS)；
The preset solution in 4 positions is cleaning solution (twice) and buffer solution eluent.
The selection of filter membrane 2：Part where filter membrane of different nature, filter membrane 2 being selected according to the difference of sample is detachable
Formula, positioned at the front end of device, can be dismantled by user.Filter membrane can select nylon, Kynoar, cellulose, poly- third
Alkene, PTFE, ceramics etc., according to the different filter membrane of the hydrophilic and hydrophobicity of sample and the impurity that may contain selection.Inhale
Nucleic acid absorption post in attached layer 3 uses earth silicon material, can specific adsorption DNA and RNA molecule, pass through regulation
The pH value of solution controls the adsorption desorption of nucleic acid molecules.
Valve a~valve d can be programme controlled magnetic valve, it would however also be possible to employ micro-fluidic micro-valve technology, by air pump control
The switch of pipeline.
It is the reagent ball after opening or translucent material, internal preset reaction mother liquor or freeze-drying above the position of reaction tank 5,
Nucleic acid eluents are mixed after flowing into reaction tank 5 with preset solution or reagent ball.Now heating module is started working, nucleic acid amplification
Reaction starts.With the progress of reaction, in solution dyestuff with formed nucleic acid molecules combined (SYBR Green detections) or
The dyestuff that being generated in solution can send in the dye molecule (Qaqman detections) or solution of fluorescence is combined with byproduct of reaction
Luminous (LAMP calcein detections), fluorescence is sent to signal transacting by light source activation, receptor detection, the signal of detection
Unit is analyzed (this part is not shown in the diagram).
SRB quantitative detection in oil field waste
Because sample is the aqueous solution, the cellulose membrane using aperture at 0.2 micron.User needs to change filter membrane part first,
Oil field waste 1mL is collected with specific sample pipe, cover is opened and puts it into device, relevant parameter is set on panel, is opened
Dynamic testing process.Whole flow process continues 30~120 minutes.
For SRB strains, kit is amplified using SRB specific nucleic acids, PCR primer therein and probe design can be with
With reference to pertinent literature or designed, designed.For LAMP reactions, there is not pertinent literature report also at present, in the market does not have yet
Finished product kit further directed to SRB 16S rDNA regions, it is necessary to carry out design of primers and exploitation.
Detection can obtain the real-time change trend of fluorescence after terminating, and can be extrapolated according to the dynamics data of reaction in sample
SRB concentration, computational methods are default in a program.As a result it may be displayed on panel, export into computer, pass through postal
Part or short message form are sent, or are directly uploaded in cloud for statistics.
It should be noted that those skilled in the art can also make such or such easy change under the teaching of this specification
Change mode, such as equivalent way, or substantially mode of texturing, all should be within protection scope of the present invention.
Claims (9)
1. the automation equipment of bacterial content in a kind of rapid measurement oilfield sewage and product oil, including casing, Enrichment of bacteria and core
Acid molecule extraction equipment, Enrichment of bacteria and the nucleic acid molecules extraction equipment includes Reagent Tube placement location (1) and Reagent Tube
It is placement location (4), filter membrane (2), adsorption layer (3) reaction tank (5), light source (6), optical filter (7), photosensitive
Device (8) and at least four valves, Reagent Tube placement location (1) bottom are connected with filter membrane (2) superjacent air space,
Filter membrane (2) underlying space is divided into two-way, is connected all the way with valve a arrival ends, and another road is connected with valve b arrival ends,
The valve a ports of export are connected with waste liquid pool, and the valve b ports of export are connected by pipeline with adsorption layer (3) entrance side, adsorption layer
(3) pipeline of outlet side is divided into two-way, is connected all the way with valve c arrival ends, and the valve c ports of export are connected with waste liquid pool,
Another road is connected after valve d with reaction tank (5)；Reagent Tube placement location (4) bottom is with passing through pipeline and valve b
Pipeline between adsorption layer entrance side is connected；Light source (6) is that reaction tank (5) provides dye molecule excitation energy, is produced
Fluorescence pass through optical filter (7) after by photoreceptor (8) receive；The top of filter membrane (2) superjacent air space, Reagent Tube are placed
The bottom of position (4) is equipped with metal spine, when Reagent Tube is placed on Reagent Tube placement location (1) or Reagent Tube placement position
The aluminium foil of reagent bottom of the tube can be punctured by putting when on (4).
2. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
In when Reagent Tube is placed on Reagent Tube placement location (1), piston is placed on Reagent Tube top, by the examination in Reagent Tube
Agent is pressed downward in the space above filter membrane (2).
3. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
Integration of equipments is caught in the casing in the Enrichment of bacteria and accounting molecule.
4. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
In valve a~valve d be programme controlled magnetic valve, or use micro-fluidic micro-valve technology, by opening for air pump control piper
Close.
5. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
Reagent ball after the top of reaction tank (5) is opening or translucent material, internal preset reaction mother liquor or freeze-drying,
Nucleic acid eluents flow into reaction tank (5) and mixed afterwards with preset solution or reagent ball.
6. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
The signal gone out in photoreceptor (8) is sent to signal processing unit and analyzed.
7. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
Higher position is placed in Reagent Tube placement location (1) or Reagent Tube placement location (4), is easy to liquid downstream to lower position.
8. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
In when using PCR method, it is necessary to quick heating-cooling module is set on reaction tank (5) periphery with realize denaturation-annealing-
The temperature cycles of extension；When using isothermal amplification method, only need to set heating module around reaction tank.
9. measuring the automation equipment of bacterial content in oilfield sewage and product oil rapidly according to claim 1, its feature exists
Completed in the replacing of Reagent Tube by automatic control system.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610163852.1A CN107216998B (en) | 2016-03-22 | 2016-03-22 | Automatic device for rapidly measuring bacterial content in oil field sewage and finished oil |
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|---|---|---|---|
| CN201610163852.1A CN107216998B (en) | 2016-03-22 | 2016-03-22 | Automatic device for rapidly measuring bacterial content in oil field sewage and finished oil |
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| CN107216998A true CN107216998A (en) | 2017-09-29 |
| CN107216998B CN107216998B (en) | 2020-05-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111569959A (en) * | 2020-04-30 | 2020-08-25 | 上海邦先医疗科技有限公司 | Micro-fluidic chip for quantitatively detecting bacteria in biological sample and use method |
| CN113176118A (en) * | 2021-04-29 | 2021-07-27 | 江苏大学 | Portable gas fax bacterium real-time acquisition and detection device and method |
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| CN103983636A (en) * | 2014-05-20 | 2014-08-13 | 中国石油化工股份有限公司 | Rapid detecting method of sulphate reducing bacteria and kit thereof |
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| CN113176118A (en) * | 2021-04-29 | 2021-07-27 | 江苏大学 | Portable gas fax bacterium real-time acquisition and detection device and method |
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