CN107213928B - A kind of micro-fluidic chip and preparation method thereof - Google Patents
A kind of micro-fluidic chip and preparation method thereof Download PDFInfo
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- CN107213928B CN107213928B CN201710401136.7A CN201710401136A CN107213928B CN 107213928 B CN107213928 B CN 107213928B CN 201710401136 A CN201710401136 A CN 201710401136A CN 107213928 B CN107213928 B CN 107213928B
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a kind of micro-fluidic chips, including substrate, sampling part and bottom cover；Substrate is equipped with sample cavity, pretreating zone, test chamber and reaction zone；Sampling part includes injection port, sample accommodating cavity and outlet；Pretreating zone includes pretreating reagent chamber, pretreatment chamber and air cavity；Reaction zone includes reaction reagent chamber and reaction chamber；Elastic cavity wall is equipped on pretreating reagent chamber, air cavity, reaction reagent chamber and reaction chamber；The upper surface of test chamber is equipped with light penetrating panel, and lower surface is equipped with light transmission bottom plate；Light penetrating panel and light transmission bottom plate are corresponding；The micro-fluidic chip, by the rapid quantitative detection of component content to be measured in microfluidic chip technology realization sample, has the characteristics that easy to operate, highly sensitive, result is accurate based on turbidimetry；The preparation method of micro-fluidic chip includes: reagent preparation and assembling chip.
Description
Technical field
The present invention relates to a kind of micro-fluidic chips and preparation method thereof, belong to chip detection technique field.
Background technique
In normal human, there are three types of hemoglobins: HbA, HbF, HbA2, and HbA is mainly contained in adult erythrocyte.?
When separating hemoglobin with chromatography, can elute 3 kinds of components containing sugars i.e.: HbA1a, HbA1b, HbA1c are collectively referred to as HbAle
Albumen.HbA1c is glucosylation hemoglobin, and other two is the saccharification Lactoferrin that other sugar are formed.Wherein the amount of HbAlc is most
Greatly, reflecting generally is indicated with HbAlc when blood glucose level, and HbAlc is formed in conjunction with glucose in the metabolic process by HbA, thus
It can accurately reflect glucose level in blood.
It is irreversible reaction that the combination of blood glucose and hemoglobin, which generates glycosylated hemoglobin, and directly proportional to blood sugar concentration,
And kept for 120 days or so, it is possible to observe the blood sugar concentration before 120 days.Glycosylated hemoglobin test usually can be anti-
Reflect nearly 8~12 weeks glycemic control situations of patient.
The meaning of detection glycosylated hemoglobin is, reflects the index of blood sugar in diabetic patients overall control situation；Sugar
Change hemoglobin normal value is 4-6%, and < 6% control is relatively low, hypoglycemia easily occurs；6-7% control is ideal；7-8% can connect
By；8-9% is that control is bad；> 9% is poor to control, and is the risk factor of generation, the development of chronic complicating diseases, and may occur
The acute complication such as ketoacidosis, thus when its > 8% control that should just reinforce blood glucose.
The prior art has low sensitivity to the diagnostic method of glycosylated hemoglobin, poor repeatability, is disturbed obviously, and
The problem that existing glycosylated hemoglobin detection necessary instrument is expensive, testing process is complicated, detection time is long.
Summary of the invention
For overcome the deficiencies in the prior art, the first purpose of this invention is to provide a kind of micro-fluidic chip, this is micro-
Fluidic chip passes through the fast quantification of component content to be measured in microfluidic chip technology realization sample based on turbidimetry
Detection, has the characteristics that easy to operate, highly sensitive, result is accurate.
Second object of the present invention is to provide a kind of preparation method of above-mentioned micro-fluidic chip.
Realize that the first purpose of this invention can reach by adopting the following technical scheme that: a kind of micro-fluidic chip, packet
Include substrate, sampling part and bottom cover；Bottom cover lid is located at the lower section of substrate；Substrate is equipped with sample cavity, pretreating zone, test chamber and anti-
Answer area；Sampling part is removably embedded in sample cavity；Sampling part includes injection port, sample accommodating cavity and outlet；
Injection port, outlet are connected to sample accommodating cavity respectively；Pretreating zone includes pretreating reagent chamber, pretreatment chamber and air cavity；In advance
Reagent treatment chamber is connected to by the first pretreating zone runner with sample cavity, and sample cavity passes through the second pretreating zone runner and pretreatment
Chamber is corresponding；Sampling part is embedded in sample cavity, and the outlet of the first pretreating zone runner is corresponding with injection port, the second pretreatment
The entrance of area's runner is corresponding with outlet；Pretreatment chamber is connected by runner with test chamber；Air cavity passes through air flue and pre- place
Manage chamber connection；Reaction zone includes reaction reagent chamber and reaction chamber；Test chamber is connected by runner with reaction chamber；Reaction reagent chamber
It is connected to by runner with reaction chamber；Elastic cavity wall is equipped on pretreating reagent chamber, air cavity, reaction reagent chamber and reaction chamber；Inspection
The upper surface for surveying chamber is equipped with light penetrating panel, and lower surface is equipped with light transmission bottom plate；Light penetrating panel and light transmission bottom plate are corresponding；The bottom of bottom cover
Face is equipped with through-hole corresponding with light transmission bottom plate.
Further, pretreating reagent chamber includes the first pretreating reagent chamber and the second pretreating reagent chamber, the first pre- place
Reason reagent chamber is connected to by the first pretreating zone runner with sample cavity；Second pretreating reagent chamber is connected by runner and pretreatment chamber
It is logical.
Further, hemolytic agent is marked in the first pretreating reagent chamber；Latex particle is marked in second pretreating reagent chamber
Suspension.
Further, latex particle suspension is prepared by following steps:
Pretreatment: the polystyrene microsphere and 1- ethyl-(3- bis- of partial size 200-400nm are added in glycine buffer
Dimethylaminopropyl) carbodiimide hydrochloride, reacts 2-3h under conditions of 20-25 DEG C, is then centrifuged for, mixes, be centrifuged repeatedly
It is operated with mixing, abandons supernatant after last time centrifugally operated, the polystyrene microsphere precipitated；
It prepares suspension: dispersing pretreated polystyrene microsphere in the solution containing hemoglobin adsorbent,
With the pH=8 of glycine buffer adjustment solution, it is then protected from light closing under conditions of 20-25 DEG C at least for 24 hours, obtains polyphenyl second
Alkene microballoon suspension.
Further, in polystyrene microsphere turbid liquid, the mass percent of polystyrene microsphere is 3%.
Further, reaction reagent chamber includes the first reaction reagent chamber and the second reaction reagent chamber；First reaction reagent chamber
Pass through runner respectively with the second reaction reagent chamber to be connected to reaction chamber.
Further, the first reaction reagent is marked in the first reaction reagent chamber；It is mono- that first reaction reagent contains the anti-HbA1c of mouse
It is anti-；The second reaction reagent is marked in second reaction reagent chamber；Second reaction reagent contains sheep anti-mouse igg antibody.
Further, waste liquid chamber is equipped between bottom cover and substrate；Test chamber is connected to by runner with waste liquid chamber.In order to examine
When survey, solution to be measured accurately measures absorbance so that incident ray, which can be worn, penetrates solution to be measured full of test chamber, then to be measured
When solution is full of test chamber, extra solution to be measured can be pressed into waste liquid chamber, the bubble in test chamber can also be released.
Realize that second object of the present invention can reach by adopting the following technical scheme that: a kind of miniflow as described above
Control the preparation method of chip, comprising the following steps:
Reagent preparation: pretreating reagent and reaction reagent are prepared respectively；
Assembling chip: injecting pretreating reagent chamber for pretreating reagent, and reaction reagent injects reaction reagent chamber, seals base
Plate, mounting base complete assembling.
Further, in assembling chip step, rubber belt sealing substrate is used.
Formulation Design Principle of the invention is as follows: based on turbidimetry, by sample ingredient to be measured and can
The reagent for reacting and generating suspended matter is reacted so that the suspended matter quantity of solutions turbid, turbidity and formation at
Direct ratio, it is also directly proportional to component content to be measured；The absorbance of its suspension is measured, the content of ingredient to be measured in sample can be found out.
Compared with prior art, the beneficial effects of the present invention are:
1, micro-fluidic chip of the invention is based on turbidimetry, by microfluidic chip technology realize in sample to
The rapid quantitative detection for surveying ingredient can pointedly be arranged the reagent that can generate suspended particulate with ingredient to be measured, detect
Light penetrating panel and light transmission bottom plate are set in chamber, can detect the intensity of incident light and emergent light, calculate the content of ingredient to be measured, have
Easy to operate, bales catch is interfered in addition to blank, has the characteristics that background influence is low, highly sensitive, result is accurate；
2, sample preprocessing, mixing, reaction, separation and detection are integrated in chip by microfluidic chip technology of the invention
On, and all reagent components needed for reaction are integrated on chip；Easy to operate, achievable the whole series detect, and detection efficiency is big
It is big to improve.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of micro-fluidic chip of the present invention；
In figure, 1, substrate；11, sample cavity；12, pretreating zone；121, the first pretreating reagent chamber；1211, the first pre- place
Manage Qu Liudao；1212, the second pretreating zone runner；122, the second pretreating reagent chamber；123, chamber is pre-processed；124, air cavity；
1241, air flue；13, test chamber；131, light penetrating panel；132, light transmission bottom plate；14, reaction zone；141, the first reaction reagent chamber；
142, the second reaction reagent chamber；143, reaction chamber；2, part is sampled；21, injection port；22, sample accommodating cavity；23, outlet；3, bottom
Lid；31, through-hole；4, elastic cavity wall；5, waste liquid chamber.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention:
Embodiment 1:
The preparation of micro-fluidic chip and for measuring HbA1c.
One, chip structure:
Referring to Fig.1, a kind of micro-fluidic chip, including substrate 1, sampling part 2 and bottom cover 3；The lid of bottom cover 3 is located under substrate 1
Side；Substrate 1 is equipped with sample cavity 11, pretreating zone 12, test chamber 13 and reaction zone 14；It is removably embedding to sample part 2
It is located in sample cavity 11；Sampling part 2 includes injection port 21, sample accommodating cavity 22 and outlet 23；Injection port 21, outlet 23 divide
Not be connected to sample accommodating cavity 22；Pretreating zone 12 includes the first pretreating reagent chamber 121, the second pretreating reagent chamber 122, pre-
Processing chamber 123 and air cavity 124；First pretreating reagent chamber 121 is connected to by the first pretreating zone runner 1211 with sample cavity 11,
Sample cavity 11 is corresponding by the second pretreating zone runner 1212 and pretreatment chamber 123, and the second pretreating reagent chamber 122 passes through stream
Road is connected to pretreatment chamber 123；Sampling part 2 is embedded in sample cavity 11, the outlet of the first pretreating zone runner 1211 and sample introduction
Mouth 21 is corresponding, and the entrance of the second pretreating zone runner 1212 is corresponding with outlet 23；It pre-processes chamber 123 and passes through runner and inspection
Chamber 13 is surveyed to be connected；Air cavity 124 is connected to by air flue 1241 with pretreatment chamber 123；Reaction zone 14 includes the first reaction reagent chamber
141, the second reaction reagent chamber 142 and reaction chamber 143；Test chamber 13 is connected by runner with reaction chamber 143；First reaction examination
Agent chamber 141 and the second reaction reagent chamber 142 are connected to by runner with reaction chamber 143 respectively；First pretreating reagent chamber 121,
On two pretreating reagent chambers 122, air cavity 124, the first reaction reagent chamber 141, the second reaction reagent chamber 142 and reaction chamber 143
Equipped with elastic cavity wall 4；The upper surface of test chamber 13 is equipped with light penetrating panel 131, and lower surface is equipped with light transmission bottom plate 132；Light penetrating panel
131 and light transmission bottom plate 132 it is corresponding；The bottom surface of bottom cover 3 is equipped with through-hole 31 corresponding with light transmission bottom plate 132；Bottom cover 3 and substrate
Waste liquid chamber 5 is equipped between 1；Test chamber 13 is connected to by runner with waste liquid chamber 5.
Runner, the first pretreating zone runner and the second pretreating zone runner among the above is the chip stream in routine techniques
Road acts on to be connected to two chambers, is the channel that reagent flow to another chamber from a chamber；Air flue is to supply in routine techniques
The channel that gas passes through, connection air cavity and pretreatment chamber.
Two, reagent is injected:
As detection glycosylated hemoglobin, need in the first pretreating reagent chamber, the second pretreating reagent chamber, the first reaction
Reagent chamber, the second reaction reagent chamber inject corresponding reagent:
Hemolytic agent is marked in first pretreating reagent chamber；Hemolytic agent includes: NaCl, tetradecyltrimethylammonium bromide
(TTAB) surfactant and stabilizer；Hemolytic agent discharges hemoglobin and saccharification blood in haemocyte for being crushed haemocyte
Lactoferrin；
Latex particle suspension is marked in second pretreating reagent chamber；
Latex particle suspension is polystyrene microsphere turbid liquid, is prepared by following steps:
Pretreatment: the polystyrene that partial size 200-400nm is added in the glycine buffer that concentration is 100mmol/L is micro-
The 1- ethyl-(3- dimethylaminopropyl) that the mass percent of ball, polystyrene microsphere is 3% and concentration is 0.5mg/mL
Carbodiimide hydrochloride reacts 2.5h under conditions of 23 DEG C, and 15min is then centrifuged under conditions of revolving speed 10000rmp, is mixed,
It is centrifuged repeatedly and is mixed operation, supernatant is abandoned after being centrifuged 25min operation under conditions of revolving speed 15000rmp for the last time, is sunk
The polystyrene microsphere in shallow lake；
It prepares suspension: dispersing pretreated polystyrene microsphere in the solution containing hemoglobin adsorbent,
The mass percent of polystyrene microsphere is 3% in solution, and glycine buffer adjusts the pH=8 of solution, then at 23 DEG C
Under the conditions of be protected from light closing for 24 hours, obtain polystyrene microsphere turbid liquid.
The first reaction reagent is marked in first reaction reagent chamber；First reaction reagent contains the anti-HbA1c monoclonal antibody of mouse；
First reaction reagent is prepared by following steps: being added in the glycine buffer that concentration is 50mmol/L
The anti-HbA1c monoclonal antibody of mouse configures the anti-HbA1c monoclonal antibody reagent of mouse, and the final concentration of antibody is greater than 0.5mg/mL in reagent；
The second reaction reagent is marked in second reaction reagent chamber；Second reaction reagent contains sheep anti-mouse igg antibody；
Second reaction reagent is prepared by following steps: being added in the glycine buffer that concentration is 50mmol/L
Sheep anti-mouse igg antibody is configured to reagent sheep anti-mouse igg antibody reagent, and the final concentration of antibody is greater than 0.5mg/mL in reagent.
Three, the preparation of chip:
The preparation method of micro-fluidic chip, it is characterised in that the following steps are included:
Reagent preparation: hemolytic agent, polystyrene microsphere turbid liquid, the anti-HbA1c monoclonal antibody reagent of mouse and sheep anti-mouse igg are anti-respectively
Body reagent；
Assembling chip: pretreating reagent is injected into pretreating reagent chamber, reaction reagent injects reaction reagent chamber, uses adhesive tape
Hermetic sealing substrate, mounting base complete assembling.
Four, application method:
1, the principle of chip is as follows: based on Immunity transmission turbidity, by Hb in sample and HbA1c with have it is identical non-
The latex particle of specific adsorption carries out immobilization, is formed after the monoclonal antibody specific of HbA1c is then added: latex granule
The compound of the anti-human HbA1c monoclonal antibody of son-HbA1c- mouse, this compound and sheep anti-mouse igg antibody act on forming agglutination, coagulate
Collection amount is different due to the difference of the immobilised HbA1c amount of latex surface；By measure its absorbance and with HbA1c percentage concentration
Standard curve relatively after, the percentage composition of the total Hb of HbA1c Zhan in sample can be found out.
2, standard curve is made:
Before detecting to sample, using purified water or physiological saline as zero point, the calibration object for preparing various concentration level is molten
Liquid repeats detection process using chip, draws standard curve.
3, include: using step
Sample-adding: taking whole blood, with heparin or EDTA anticoagulation, stands 3h or more or 2000rpm and is centrifuged through 2min, then from red
It accurately takes 10 μ L as sample in cellular layer, sampling part is taken out, be added from the injection port of sampling part into sample accommodating cavity, so
Sampling part is inserted into the sample cavity of substrate afterwards；
Pretreatment: squeeze the first pretreating reagent chamber elastic cavity wall, then hemolytic agent from the first pretreating zone runner into
Sample mouth, and sample accommodating cavity is rinsed, sample and hemolytic agent mix the second pretreating zone of merga pass runner and flow to pretreatment chamber；It squeezes
Polystyrene microsphere turbid liquid is pressed into pretreatment chamber and mixed with sample, in sample by the elastic cavity wall of the second pretreating reagent chamber
Total Hb and HbA1c and polystyrene microsphere generate non-specific adsorption and immobilization, obtain preprocessing solution；
Blank detection: the elastic cavity wall of air cavity is squeezed, preprocessing solution is promoted test chamber by the gas in air cavity, and solution fills
Blank detection is carried out when full test chamber, preprocessing solution passes through test chamber under the influence of gas, flow to reaction chamber with channel；
Reaction: the elastic cavity wall of the first reaction reagent chamber and the second reaction reagent chamber is squeezed, by the anti-HbA1c monoclonal antibody reagent of mouse
Reaction chamber is pressed into sheep anti-mouse igg antibody reagent to be reacted, and the anti-human HbA1c Dan Ke of polystyrene microsphere-HbA1c- mouse is formed
The compound of grand antibody, this compound and sheep anti-mouse igg antibody act on forming agglutination, and agglutination amount is solid because of Surfaces of Polystyrene Microparticles
The difference of the HbA1c amount of phaseization and it is different, obtain solution to be measured:
Detection: after reaction, the cavity wall of extruding reaction chamber, solution to be measured is back in test chamber, solution filling detection to be measured
Chamber injects incident light from light penetrating panel, light transmission bottom plate monitoring emergent light, incident light using 660nm monochromatic light as dominant wavelength,
For 800nm monochromatic light as commplementary wave length, the absorbance value A surveyed out calculates sample compared with the standard curve of HbA1c percentage concentration
HbA1c percentage composition in this.
4, the range of linearity is tested
High low value sample preparation: high level sample (1#): the analyte concentration that sample contains is 16%, micro-fluidic to be higher by
Chip detects the 14% of limit value.Low value sample (2#): the analyte concentration that sample contains is 1.5%.
Intermediate 5 concentration samples preparation: 1# and 2# sample is matched by the mixing of 5:1,4:2,3:3,2:4,1:5 volume ratio respectively
It is set to 3#, 4#, 5#, 6#, 7# linear sample.
Random sequence is by 1-7# sample retest 3 times.
The result shows that range of linearity 2-14%, R >=0.9900, and batch interior repeatability is preferable, in sample HbA1c
Mass percent is standard deviation 0.075% at 4.8%, the coefficient of variation 1.56%, in sample HbA1c% mass percentage
Than at 10.6%, as a result standard deviation 0.11%, the coefficient of variation 1.1% meets clinical demand, can be diabetic
Diagnosis provides reference.
The principle that the present invention uses antigen and antibody specific to combine, antibody (Ab) are reacted with soluble antigen (Ag), are formed
The immune complex of certain structure, becomes the particle being suspended in reaction solution.The compound particles formed in the reaction have
Special optical property can be detected with instrument, improve speed, sensitivity and the ease for operation of detection.
For those skilled in the art, it can make other each according to the above description of the technical scheme and ideas
Kind is corresponding to be changed and deforms, and all these change and deform the protection model that all should belong to the claims in the present invention
Within enclosing.
Claims (9)
1. a kind of micro-fluidic chip, including substrate, sampling part and bottom cover；The bottom cover lid is located at the lower section of substrate；Its feature exists
In the substrate is equipped with sample cavity, pretreating zone, test chamber and reaction zone；The sampling part is removably embedded
In sample cavity；The sampling part includes injection port, sample accommodating cavity and outlet；The injection port, outlet are respectively and sample
The connection of this accommodating cavity；The pretreating zone includes pretreating reagent chamber, pretreatment chamber and air cavity；The pretreating reagent chamber passes through
First pretreating zone runner is connected to sample cavity, and sample cavity is corresponding with pretreatment chamber by the second pretreating zone runner；Sampling
Part is embedded in sample cavity, and the outlet of the first pretreating zone runner is corresponding with injection port, the entrance of the second pretreating zone runner
It is corresponding with outlet；The pretreatment chamber is connected by runner with test chamber；The air cavity passes through air flue and pretreatment chamber
Connection；The reaction zone includes reaction reagent chamber and reaction chamber；The test chamber is connected by runner with reaction chamber；It is described anti-
Reagent chamber is answered to be connected to by runner with reaction chamber；It is equipped on the pretreating reagent chamber, air cavity, reaction reagent chamber and reaction chamber
Elastic cavity wall；The upper surface of the test chamber is equipped with light penetrating panel, and lower surface is equipped with light transmission bottom plate；The light penetrating panel and light transmission
Bottom plate is corresponding；The bottom surface of the bottom cover is equipped with through-hole corresponding with light transmission bottom plate.
2. micro-fluidic chip as described in claim 1, which is characterized in that the pretreating reagent chamber includes the first pretreatment examination
Agent chamber and the second pretreating reagent chamber, the first pretreating reagent chamber are connected to by the first pretreating zone runner with sample cavity；Second
Pretreating reagent chamber is connected to by runner with pretreatment chamber.
3. micro-fluidic chip as claimed in claim 2, which is characterized in that be marked with haemolysis in the first pretreating reagent chamber
Agent；Latex particle suspension is marked in the second pretreating reagent chamber.
4. micro-fluidic chip as claimed in claim 3, which is characterized in that the latex particle suspension passes through following steps system
It is standby to obtain:
Pretreatment: being added the polystyrene microsphere of partial size 200-400nm in the glycine buffer that concentration is 100 mmol/L,
1- ethyl-(3- dimethylaminopropyl) carbon two that the mass percent of polystyrene microsphere is 3% and concentration is 0.5mg/mL
Inferior amine salt hydrochlorate reacts 2-3h under conditions of 20-25 DEG C, is then centrifuged for, mixes, and is centrifuged repeatedly and mixes operation, last
Supernatant is abandoned after secondary centrifugally operated, the polystyrene microsphere precipitated；
It prepares suspension: dispersing pretreated polystyrene microsphere in the solution containing hemoglobin adsorbent, use is sweet
Propylhomoserin buffer adjusts the pH=8 of solution, and closing is then protected from light under conditions of 20-25 DEG C at least for 24 hours, obtains polystyrene
Microballoon suspension.
5. micro-fluidic chip as described in claim 1, which is characterized in that the reaction reagent chamber includes the first reaction reagent chamber
With the second reaction reagent chamber；The first reaction reagent chamber and the second reaction reagent chamber pass through runner respectively and are connected to reaction chamber.
6. micro-fluidic chip as claimed in claim 5, which is characterized in that be marked with the first reaction in the first reaction reagent chamber
Reagent；First reaction reagent contains the anti-HbA1c monoclonal antibody of mouse；The second reaction reagent is marked in the second reaction reagent chamber；
Second reaction reagent contains sheep anti-mouse igg antibody.
7. micro-fluidic chip as described in claim 1, which is characterized in that be equipped with waste liquid chamber between the bottom cover and substrate；Institute
Test chamber is stated to be connected to by runner with waste liquid chamber.
8. a kind of preparation method of micro-fluidic chip as described in claim 1, it is characterised in that the following steps are included:
Reagent preparation: pretreating reagent and reaction reagent are prepared respectively；
Assembling chip: pretreating reagent is injected into pretreating reagent chamber, reaction reagent injects reaction reagent chamber, hermetic sealing substrate, peace
Bottom plate is filled, assembling is completed.
9. the preparation method of micro-fluidic chip as claimed in claim 8, which is characterized in that in assembling chip step, use glue
Band hermetic sealing substrate.
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| CN108490182B (en) * | 2018-02-06 | 2021-04-30 | 深圳市第二人民医院 | Glycosylated hemoglobin detection device and detection method |
| CN110560184A (en) * | 2018-06-06 | 2019-12-13 | 厦门大学 | Microfluidic chip, microfluidic reaction system and driving method |
| TW202102850A (en) * | 2019-03-29 | 2021-01-16 | 日商積水醫療股份有限公司 | Immunoassay reagent and immunoassay method |
| CN110568200B (en) * | 2019-09-12 | 2022-05-31 | 重庆科技学院 | Combined diagnosis paper-based micro-fluidic chip and detection method |
| CN110658166B (en) * | 2019-09-30 | 2022-03-25 | 重庆科技学院 | Micro-fluidic chip and system thereof, and detection method of heavy metal ions in water body |
| CN110702894A (en) * | 2019-10-10 | 2020-01-17 | 南京欧凯生物科技有限公司 | Detection method for directly measuring content of glycosylated hemoglobin |
| CN115867802A (en) * | 2021-06-08 | 2023-03-28 | 京东方科技集团股份有限公司 | First substrate, microfluidic chip and sample processing method |
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| EP2064346B1 (en) * | 2006-09-06 | 2013-11-06 | Canon U.S. Life Sciences, Inc. | Chip and cartridge design configuration for performing micro-fluidic assays |
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