CN107208314B - 一种单体型分型测序文库的构建方法、分型方法和试剂 - Google Patents
一种单体型分型测序文库的构建方法、分型方法和试剂 Download PDFInfo
- Publication number
- CN107208314B CN107208314B CN201580068011.6A CN201580068011A CN107208314B CN 107208314 B CN107208314 B CN 107208314B CN 201580068011 A CN201580068011 A CN 201580068011A CN 107208314 B CN107208314 B CN 107208314B
- Authority
- CN
- China
- Prior art keywords
- sites
- enzyme
- sequencing library
- linker
- snp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000012163 sequencing technique Methods 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 20
- 238000010276 construction Methods 0.000 title claims abstract description 19
- 102000054766 genetic haplotypes Human genes 0.000 title claims description 38
- 239000012634 fragment Substances 0.000 claims abstract description 63
- 102000053602 DNA Human genes 0.000 claims abstract description 49
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 35
- 230000003321 amplification Effects 0.000 claims abstract description 27
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 27
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 24
- 230000007017 scission Effects 0.000 claims abstract description 24
- 238000007403 mPCR Methods 0.000 claims abstract description 18
- 108020004638 Circular DNA Proteins 0.000 claims abstract description 17
- 210000000349 chromosome Anatomy 0.000 claims abstract description 17
- 108020004414 DNA Proteins 0.000 claims description 34
- 238000006243 chemical reaction Methods 0.000 claims description 27
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 238000012408 PCR amplification Methods 0.000 claims description 20
- 238000013519 translation Methods 0.000 claims description 14
- 238000007363 ring formation reaction Methods 0.000 claims description 6
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 108060002716 Exonuclease Proteins 0.000 claims description 3
- 102000003960 Ligases Human genes 0.000 claims description 3
- 108090000364 Ligases Proteins 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 claims description 3
- 102000013165 exonuclease Human genes 0.000 claims description 3
- 230000029087 digestion Effects 0.000 claims description 2
- 238000001976 enzyme digestion Methods 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 47
- 239000000243 solution Substances 0.000 description 16
- 235000019441 ethanol Nutrition 0.000 description 15
- 239000011324 bead Substances 0.000 description 13
- 239000000047 product Substances 0.000 description 11
- 239000008280 blood Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 239000011541 reaction mixture Substances 0.000 description 10
- 238000001179 sorption measurement Methods 0.000 description 9
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 6
- 238000003287 bathing Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 101000690100 Homo sapiens U1 small nuclear ribonucleoprotein 70 kDa Proteins 0.000 description 4
- 101100029173 Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) SNP2 gene Proteins 0.000 description 4
- 101100094821 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SMX2 gene Proteins 0.000 description 4
- 102100024121 U1 small nuclear ribonucleoprotein 70 kDa Human genes 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 239000012224 working solution Substances 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 239000002096 quantum dot Substances 0.000 description 3
- 101710095468 Cyclase Proteins 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- 238000012165 high-throughput sequencing Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 125000000449 nitro group Chemical class [O-][N+](*)=O 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000143060 Americamysis bahia Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 101100236128 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LSM2 gene Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 208000005980 beta thalassemia Diseases 0.000 description 1
- 238000009582 blood typing Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (13)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2015/070143 WO2016109928A1 (zh) | 2015-01-06 | 2015-01-06 | 一种单体型分型测序文库的构建方法、分型方法和试剂 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107208314A CN107208314A (zh) | 2017-09-26 |
CN107208314B true CN107208314B (zh) | 2020-06-16 |
Family
ID=56355392
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201580068011.6A Active CN107208314B (zh) | 2015-01-06 | 2015-01-06 | 一种单体型分型测序文库的构建方法、分型方法和试剂 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN107208314B (zh) |
WO (1) | WO2016109928A1 (zh) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108985009B (zh) * | 2018-08-29 | 2022-06-07 | 北京希望组生物科技有限公司 | 一种获得基因单体型序列的方法及其应用 |
CN109576799B (zh) * | 2018-11-30 | 2022-04-26 | 深圳安吉康尔医学检验实验室 | Fh测序文库的构建方法和引物组及试剂盒 |
CN112795620A (zh) * | 2019-11-13 | 2021-05-14 | 深圳华大基因股份有限公司 | 双链核酸环化方法、甲基化测序文库构建方法和试剂盒 |
CN114250279B (zh) * | 2020-09-22 | 2024-04-30 | 上海韦翰斯生物医药科技有限公司 | 一种单倍型的构建方法 |
CN113981048B (zh) * | 2021-08-30 | 2024-04-30 | 司法鉴定科学研究院 | 一种基于二代测序技术检测微单倍型基因座的引物组合物、试剂盒和方法及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102199668A (zh) * | 2011-04-15 | 2011-09-28 | 上海交通大学 | 纳米颗粒分子单倍型分型方法 |
CN102864498A (zh) * | 2012-09-24 | 2013-01-09 | 天津工业生物技术研究所 | 一种长片段末端文库的构建方法 |
CN102925471A (zh) * | 2012-08-10 | 2013-02-13 | 内蒙古科技大学 | 非限制性构建无缝质粒表达载体的方法 |
WO2013071520A1 (zh) * | 2011-11-18 | 2013-05-23 | 深圳华大基因科技有限公司 | 用于病毒检测的方法和*** |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102181519B (zh) * | 2011-02-16 | 2014-01-01 | 上海交通大学 | 利用环化RNase H检测基因多态性SNP位点的方法 |
CN102181443B (zh) * | 2011-03-21 | 2013-10-23 | 中国科学院植物研究所 | 一种多重检测基因组dna多态性的方法及其专用探针 |
CN103146816B (zh) * | 2013-02-05 | 2014-08-20 | 南京大学 | 一种鉴定外来入侵物种互花米草种群的dna分子标记方法 |
-
2015
- 2015-01-06 WO PCT/CN2015/070143 patent/WO2016109928A1/zh active Application Filing
- 2015-01-06 CN CN201580068011.6A patent/CN107208314B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102199668A (zh) * | 2011-04-15 | 2011-09-28 | 上海交通大学 | 纳米颗粒分子单倍型分型方法 |
WO2013071520A1 (zh) * | 2011-11-18 | 2013-05-23 | 深圳华大基因科技有限公司 | 用于病毒检测的方法和*** |
CN102925471A (zh) * | 2012-08-10 | 2013-02-13 | 内蒙古科技大学 | 非限制性构建无缝质粒表达载体的方法 |
CN102864498A (zh) * | 2012-09-24 | 2013-01-09 | 天津工业生物技术研究所 | 一种长片段末端文库的构建方法 |
Also Published As
Publication number | Publication date |
---|---|
CN107208314A (zh) | 2017-09-26 |
WO2016109928A1 (zh) | 2016-07-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK2002017T3 (en) | High-capacity detection of molecular markers based on restriction fragments | |
DK2789696T3 (en) | A method for high-throughput AFLP-based polymorphism | |
CN107208314B (zh) | 一种单体型分型测序文库的构建方法、分型方法和试剂 | |
EP1587946B1 (en) | Haplotype analysis | |
US20160060686A1 (en) | Strategies for high throughput identification and detection of polymorphisms | |
US20190106738A1 (en) | Dna amplification method | |
US10100351B2 (en) | High-throughput sequencing detection method for methylated CpG islands | |
US20140206552A1 (en) | Methods for preimplantation genetic diagnosis by sequencing | |
WO2014101655A1 (zh) | 一种高通量核酸分析方法及其应用 | |
WO2013052557A2 (en) | Methods for preimplantation genetic diagnosis by sequencing | |
CN108060227B (zh) | 一种检测pah基因突变的扩增引物、试剂盒及其检测方法 | |
WO2019085320A1 (en) | Methods and kits for targeted enrichment of target dna with high gc content | |
AU647806B2 (en) | Genomic mapping method by direct haplotyping using intron sequence analysis | |
Kulski et al. | In phase HLA genotyping by next generation sequencing-a comparison between two massively parallel sequencing bench-top systems, the Roche GS Junior and ion torrent PGM | |
CN109355288B (zh) | 一种目标dna富集的方法和应用 | |
Amr et al. | Targeted Hybrid Capture for Inherited Disease Panels | |
KR20090111407A (ko) | 인간 성염색체 짧은 연쇄반복 염기서열의 동시증폭 키트 및방법 | |
Obeidova et al. | Improvement of Diagnostic Yield by an Additional Amplicon Module to Hybridization-Based Next-Generation Sequencing Panels | |
CN114686580A (zh) | 用于核酸样本扩增的组合物、试剂盒、方法及*** | |
CN114686579A (zh) | 用于核酸样本扩增的组合物、试剂盒、方法及*** | |
Ruff | Genotyping by multiplexed sequencing: a robust and adaptable genotyping technology for wheat | |
CN114686562A (zh) | 用于核酸样本扩增的组合物、试剂盒、方法及*** | |
CN114686561A (zh) | 用于核酸样本扩增的组合物、试剂盒、方法及*** | |
Kamberov et al. | Use of in vitro OmniPlex libraries for high-throughput comparative genomics and molecular haplotyping | |
JP2024002889A (ja) | Dnaライブラリーの作製キット及び作製方法、遺伝子多型の検出方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1240288 Country of ref document: HK |
|
CB02 | Change of applicant information |
Address after: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Shenzhen, Guangdong Applicant after: BGI SHENZHEN Address before: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Shenzhen, Guangdong Applicant before: BGI SHENZHEN |
|
CB02 | Change of applicant information | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20180530 Address after: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong. Applicant after: MGI TECH Co.,Ltd. Address before: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Shenzhen, Guangdong Applicant before: BGI SHENZHEN |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee after: Shenzhen Huada Zhizao Technology Co.,Ltd. Address before: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee before: MGI TECH Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210608 Address after: 518000 2 / F, building 11, Beishan Industrial Zone, Yantian street, Yantian District, Shenzhen City, Guangdong Province Patentee after: Shenzhen Huada Zhizao Software Technology Co.,Ltd. Address before: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong. Patentee before: Shenzhen Huada Zhizao Technology Co.,Ltd. |