CN1072020A - Multipurpose blood diluent - Google Patents
Multipurpose blood diluent Download PDFInfo
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- CN1072020A CN1072020A CN 91109885 CN91109885A CN1072020A CN 1072020 A CN1072020 A CN 1072020A CN 91109885 CN91109885 CN 91109885 CN 91109885 A CN91109885 A CN 91109885A CN 1072020 A CN1072020 A CN 1072020A
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- blood
- haemocyte
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Abstract
The present invention provides a kind of multipurpose blood diluent that is used for blood count and stereometry.This thinning agent is that chemical salt is formed stable electrolytic solution, oozes with respect to the solution in the haemocyte etc., and the pH value is compared with haemocyte, also is balance in essence.This thinning agent uses the citrate of potassium and sodium as electrolyte, pH value buffering agent, blood anticoagulant, makes antiseptic with the broad-spectrum antibiotic gentamicin.Be used for corresponding particle counting determining instrument, can carry out the mensuration of the various parameters of haemocyte, and good anti-freezing and fungistatic effect are arranged.
Description
The present invention relates to a kind of blood cell particle counting and volumetric multipurpose blood diluent of being used for.
It is the general medical science diagnosis that the patient is carried out the blood sample analysis chemical examination, determines one of main means of illness.Need add certain blood thinners when carrying out blood sample analysis, it must comprise suitable electrolyte ingredient, and must be the osmotic pressure balance with respect to haemocyte solution.For suppressing microbial growth, also want adding preservative agent, and the introducing of antiseptic may cause the variation of blood cell volume, for balance or compensation antiseptic to the influence that blood cell volume changes, also need add activator.Because of the pH value of blood generally about 7.4, be balance so also require the pH value of used blood thinners and the pH value of haemocyte solution.European patent 81107792.4 and 8311316.0 disclosed multipurpose blood diluents, its common trait are that the chloride with sodium and potassium is a dielectric, and hydrogen and potassium dihydrogen, na phosphates are the pH buffering agent, disodium salt disodium (EDTANa
2) control bivalent positive ion, difference be the latter be with the 2-phenoxetol as antiseptic, make surperficial activator with polyoxyethylene-polyoxypropylene ethylene glycol.The former makes antiseptic with phenylethanol, and lithium chloride is made the erythrocyte volume stabilizing agent.The prior art weak point except formula components than the complexity, all unresolved anti-freezing problem, the actual blood thinners that uses are at room temperature stored the agglutination phenomenon that can occur haemocyte behind the certain hour, cause sample-out count higher, influence the accuracy of blood count and mensuration, even can't use.Except, the former makes the erythrocyte volume stabilizing agent because of introducing lithium chloride, and introduce more inorganic salts, easily the metallic element to measuring system produces corrosion product, the inorganic salts crystallization, cause the obstruction of conduit and counting hole, the latter also produces the aeration enumeration of thrombocytes because of the cleaning function of 2-phenylethanol.At present domestic commercially available be PC601 type blood platelet, the matching used thinning agent of red-cell count instrument that Japanese ERMAIN produces, its prescription is not seen open, and price is expensive, the anti-freezing weak effect, erosion resistance is low, uses the later stage even can't use, and causes waste.
The object of the present invention is to provide that a kind of formula components is simple, anti-freezing is effective, the multipurpose blood diluent that erosion resistance is strong, result of use is good.
The present invention is achieved in that a kind ofly oozes with respect to haemocyte solution etc., the multipurpose blood diluent of pH value and haemocyte balance comprises sodium chloride 6.30～8.55g/l, sodium citrate 1.39～4.17g/l, potassium citrate 0.41～1.23g/l, gentamicin 4000～10000u/l(1u=1 μ g), said components distilled water diluting.
Sodium chloride, potassium citrate, sodium are formed the electrolytic solution of osmotic pressure balance among the present invention, potassium citrate and sodium, the calcium ion of displacement blood aggegation system, K
+, Na
+Ion energy stabilised blood cellular morphology so potassium citrate and sodium had both played anticoagulation, plays the effect of stabilised blood cell volume again.Gentamicin is the antibiotic of wide spectrum, has stronger bactericidal action, except that favorable anti-corrosion effect, does not also influence erythrocyte volume stability.Owing to adopted the inorganic salts composition of trying one's best few, the pH value is in neutrality, and is with the pH value balance of blood of human body, therefore little to the metallic element corrosivity of measuring system in essence, alleviated the generation that corrosion produces, the obstruction of conduit, counting hole has been had a tangible improvement.
Now in conjunction with the embodiments the present invention is done and describe in further detail: the preferential component concentration of selecting of the present invention is sodium chloride (AR) 8.10g/l, sodium citrate (AR) 2.98g/l, potassium citrate (AR) 0.82g/l, gentamicin 6000u/l(u is an international unit, 1u=1 μ g), be diluted to 1000ml with fresh DDW.Preparing multipurpose blood diluent of the present invention does not need special process, and each composition can random order adds, fully dissolving, but need to filter through G4 bacterium funnel, use 0.3 μ and 0.4 μ filter membrane pressure filtration again after seasoning in packing container.During use, the pipeline of measuring system, container, packing container etc. must use fresh DDW to clean up.
Multipurpose blood diluent provided by the present invention can be used for measuring the corresponding instrument of various blood counts, PC601 for example, PC604 etc., automatically handle patient's blood sample continuously, red blood cell count(RBC) (RBC), packed cell volume (HCT), haemoglobin (HGB), platelet count, white blood cell count(WBC) (WBC) can be measured, mean corpuscular volume (MCV) (MCV), mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular hemoglobin (MCH) can also be measured as calculated.
(1) sample-out count experiment:
Blood cell counting instrument itself requires sample-out count to be preferably zero to dilution, Gao Buying＞3.Use the present invention to be used for the PC601 instrument, record sample-out count and instrument required standard value is as shown in the table:
| Show number of times | 1 | 2 | 3 | 4 |
| Standard value | 0.3 | 0.3 | 0.9 | 3.0 |
| Material is surveyed the thinning agent sample-out count | 0.23 | 0.29 | 0.86 | 2.8 |
Annotate: the sample-out count of actual measurement thinning agent is the mean value of 20 countings.As seen from the above table, the sample-out count in the multipurpose blood diluent of the present invention all meets instrument itself to blood thinners required standard value.
(2) use the thinning agent (ERMA) of instrument adapted as shown in the table with the contrast of thinning agent mensuration platelet count of the present invention:
Annotate: X is the mean value of 12 countings, and SD is a standard deviation.The enumeration of thrombocytes result is quite approaching in two kinds of thinning agents.
(3) anti-freezing effect comparison experiment:
Anti-freezing effect of the present invention is better than the blood thinners of instrument adapted.
(4) stability experiment
Blood sample collection is measured immediately and is placed after 24 hours after with two kinds of diluent treatment and measures platelet count, and the result is as follows:
X wherein
1, X
2Be the mean value of 20 countings.
Blood sample collection dilutes in two kinds of reagent respectively, all measure immediately respectively with room temperature under place and to measure after 24 hours, observe that haemocyte all is sunken to the pipe end, no finding of naked eye haemolysis in the two.Again stir evenly laggard promoting circulation of blood platelet number, find that it (is X than instant counting height
2＞X
1), but thinning agent X of the present invention
2/ X
1Than low 0.13 times of ERMA reagent, promptly its stability is better than ERMA reagent.
Blood thinners of the present invention also has tangible fungistatic effect through using proof, and the pair cell form do not have obvious influence, and measurement result has had tangible improvement to the conduit of measuring system, the stopping state of counting hole accurately and reliably.
In sum, compared with the prior art multipurpose blood diluent provided by the present invention has the following advantages and effect:
(1) selects few composition of trying one's best in the prescription, be used for corresponding count measurement instrument, can record the various parameters of blood cell;
(2) adopt the citrate of potassium and sodium to have the effect of good anti-freezing and stabilised blood cell volume;
(3) gentamicin is made antiseptic and not only can be suppressed growth of microorganism effectively, and blood cell shape is not had obvious influence, and measurement result accurately and reliably;
(4) use few inorganic salts of trying one's best, alleviated counting measuring system plugging, plug-hole phenomenon significantly.
Claims (2)
1, a kind of is etc. to ooze with respect to haemocyte solution, and the multipurpose blood diluent of the sodium chloride-containing of pH value and haemocyte balance is characterized in that this thinning agent comprises:
Sodium chloride 6.30～8.55g/l,
Sodium citrate 1.39～4.17g/l,
Potassium citrate 0.41～1.23g/l,
Gentamicin 4000～10000u/l,
The said components distilled water diluting.
2, according to the said a kind of multipurpose blood diluent of claim 1, it is characterized in that:
Sodium chloride 8.10g/l,
Sodium citrate 2.98g/l,
Sodium citrate 0.82g/l,
Gentamicin 6000u/l,
The said components distilled water diluting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91109885 CN1072020A (en) | 1991-10-28 | 1991-10-28 | Multipurpose blood diluent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91109885 CN1072020A (en) | 1991-10-28 | 1991-10-28 | Multipurpose blood diluent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1072020A true CN1072020A (en) | 1993-05-12 |
Family
ID=4910033
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91109885 Pending CN1072020A (en) | 1991-10-28 | 1991-10-28 | Multipurpose blood diluent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1072020A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100376669C (en) * | 2005-02-25 | 2008-03-26 | 深圳迈瑞生物医疗电子股份有限公司 | Treatment method for biological erythrocyte volume long time stabilization |
| WO2010118706A2 (en) | 2009-04-17 | 2010-10-21 | Centro De Neurociencias De Cuba | Method for obtaining novel derivatives of naphthalene for the in vivo diagnosis of alzheimer's disease |
| CN101685050B (en) * | 2008-09-26 | 2012-07-04 | 希森美康株式会社 | Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same |
| CN103323308A (en) * | 2013-05-23 | 2013-09-25 | 北京博晖创新光电技术股份有限公司 | Reagent for processing trace whole blood and application thereof |
| CN106168554A (en) * | 2016-02-29 | 2016-11-30 | 嘉善加斯戴克医疗器械有限公司 | A kind of reagent for hemocyte analyzers |
-
1991
- 1991-10-28 CN CN 91109885 patent/CN1072020A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100376669C (en) * | 2005-02-25 | 2008-03-26 | 深圳迈瑞生物医疗电子股份有限公司 | Treatment method for biological erythrocyte volume long time stabilization |
| CN101685050B (en) * | 2008-09-26 | 2012-07-04 | 希森美康株式会社 | Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same |
| WO2010118706A2 (en) | 2009-04-17 | 2010-10-21 | Centro De Neurociencias De Cuba | Method for obtaining novel derivatives of naphthalene for the in vivo diagnosis of alzheimer's disease |
| EP2860169A2 (en) | 2009-04-17 | 2015-04-15 | Centro De Neurociencias De Cuba | Method for obtaining novel derivatives of naphtalene for the in vivo diagnosis of Alzheimer's disease |
| CN103323308A (en) * | 2013-05-23 | 2013-09-25 | 北京博晖创新光电技术股份有限公司 | Reagent for processing trace whole blood and application thereof |
| CN103323308B (en) * | 2013-05-23 | 2016-01-06 | 北京博晖创新光电技术股份有限公司 | A kind of reagent and application thereof processing micro whole blood |
| CN106168554A (en) * | 2016-02-29 | 2016-11-30 | 嘉善加斯戴克医疗器械有限公司 | A kind of reagent for hemocyte analyzers |
| CN106168554B (en) * | 2016-02-29 | 2019-10-18 | 迈克生物股份有限公司 | A kind of reagent for hemocyte analyzers |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
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