CN107201557A - 具有非线性力学性能的静电纺丝组织工程血管的接收装置 - Google Patents

具有非线性力学性能的静电纺丝组织工程血管的接收装置 Download PDF

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CN107201557A
CN107201557A CN201710309611.8A CN201710309611A CN107201557A CN 107201557 A CN107201557 A CN 107201557A CN 201710309611 A CN201710309611 A CN 201710309611A CN 107201557 A CN107201557 A CN 107201557A
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李倩
王小峰
徐轶洋
牛宗武
高艳红
李旭燕
蒋晶
蒋林
侯建华
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Zhengzhou University
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    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
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Abstract

本发明提出一种具有非线性力学性能的静电纺丝组织工程血管的接收装置，解决了现有技术生长的组织工程血管与真实血管性能匹配度不高的问题。所述接收装置包括推进器，推进器与喷丝口相连，喷丝口处设有静电高压施加装置，喷丝口处设有收集装置，收集装置包括旋转轴和在水平面内往复运动的移动轴。现有技术中，利用静电纺丝技术制备的组织工程血管应力应变曲线与真实血管的需求还有差距，主要是前者没有低应力大应变的区域。本发明通过改善接收装置使收集到的纤维发生一定程度的弯曲，进而使所制备的血管在变形初期可以依靠纤维弯曲部分的伸直来实现区域I，在较小的应力情况下血管能够出现较大的扩张，以适应瞬间的血流增大等情况。

Description

具有非线性力学性能的静电纺丝组织工程血管的接收装置
技术领域
本发明涉及组织工程血管制备领域，具体涉及一种静电纺丝技术制备工程血管的装置。
背景技术
心血管疾病是人类健康的头号杀手，组织工程血管是实现病患部位血管替代或再生的理想选择。在众多组织工程血管制备工艺中静电纺丝技术因操作简便、制品孔隙率高、拥有适宜细胞生长的纳米结构等特点而受到学者们的青睐，其制备原理如图1所示。聚合物溶液由推进器2输送到喷丝口3，喷丝口3施加的静电高压使具有一定粘性的聚合物溶液在电场斥力的作用下开始劈裂成纤维(直径为几百纳米)，并向零电势处(收集装置4)运动。随着电纺的进行，纤维最终在收集装置处堆砌成管状制品，使其脱离收集装置后即成组织工程血管，如图2所示。
这类收集装置多为可转动的单金属管，此种接收方式所制备的组织工程血管与真实血管在力学特性上有一定的差别，即仅有一段线性区域。而真实血管拉伸应力应变曲线有两段弹性区域，如图3所示。其中区域I为低模量区，区域II为高模量区，低模量区主要是为了适应血流量突然增加(如情绪波动、剧烈运动等)所需求的血管适度扩张，高模量区则可以限制血管无限制的扩张。利用现有的收集装置所制备的组织工程血管仅有一个弹性区域，不能很好地与真实血管的性能相匹配。
发明内容
本发明提出一种具有非线性力学性能的静电纺丝组织工程血管的接收装置，解决了现有技术生长的组织工程血管与真实血管性能匹配度不高的问题。
本发明的技术方案是以下述方式实现的：一种具有非线性力学性能的静电纺丝组织工程血管的接收装置，包括推进器，推进器与喷丝口相连，喷丝口处设有静电高压施加装置，喷丝口处设有收集装置，收集装置包括旋转轴和在水平面内往复运动的移动轴。
非线性力学性能的静电纺丝组织工程血管的接收方式是按照下述方式进行的：推进器将聚合物溶液输送到喷丝口处，静电高压施加装置在喷丝口处施加静电高压，聚合物溶液在电场斥力的作用下劈裂成纤维，并向收集装置处运动，纤维搭接在旋转轴和移动轴上，移动轴往复运动时，纤维经历拉伸及应力松弛，产生弯曲纤维，弯曲纤维再由旋转轴缠绕堆砌成管。
优选的，配置10％浓度的聚乳酸纺丝液，推进器将聚乳酸纺丝液输送至静电高压施加装置，旋转轴和移动轴之间距离为3cm，移动轴运动的周期为2秒、振幅为1.5cm，旋转轴的角速度为2rad/s。
现有技术中，利用静电纺丝技术制备的组织工程血管应力应变曲线与真实血管的需求还有差距，主要是前者没有低应力大应变的区域。本发明通过改善接收装置使收集到的纤维发生一定程度的弯曲，进而使所制备的血管在变形初期可以依靠纤维弯曲部分的伸直来实现区域I，在较小的应力情况下血管能够出现较大的扩张，以适应瞬间的血流增大等情况。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1为静电纺丝组织工程血管制备原理图。
图2为现有技术的静电纺丝组织工程血管。
图3为现有技术中组织工程血管与真实血管力学性能的对比图。
图4为本发明接收装置的原理图。
图5为纤维弯曲原理图。
图6为弯曲的纤维的扫描电镜图。
图7为改良后血管的应力应变曲线图。
图8为实施例中所得产品的微观形貌图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有付出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
如图4所示，一种具有非线性力学性能的静电纺丝组织工程血管的接收装置，包括推进器2，推进器2与喷丝口3相连，喷丝口3处设有静电高压施加装置1，喷丝口3处设有收集装置4，收集装置4包括旋转轴4-1和在水平面内往复运动的移动轴4-2(移动轴4-2可以在水平面内往复移动，但是不能旋转)。一般静电高压施加装置1施加的静电高压为4-40千伏，在实际生产中静电高压不超过100千伏。
所述接收装置是按照下述方式运行的：推进器2将聚合物溶液输送到喷丝口3处，静电高压施加装置1在喷丝口3处施加静电高压，聚合物溶液在电场斥力的作用下劈裂成纤维，并向收集装置4处运动，纤维5搭接在旋转轴4-1和移动轴4-2上，移动轴4-2往复运动时，纤维经历拉伸及应力松弛，产生弯曲纤维，弯曲纤维再由旋转轴4-1缠绕堆砌成管。
本发明由两根接地的金属轴(旋转轴4-1和移动轴4-2)组成组成，旋转轴4-1位置固定并且可以旋转，移动轴4-2可以在水平面内做往复运动，往复运动的频率和振幅可调。
接收纤维时，旋转轴4-1和移动轴4-2起到平行板电极的作用，纤维会垂直于旋转轴4-1和移动轴4-2的平行方向搭接于其上。当移动轴4-2完成一次往复运动后，纤维在经历拉伸及应力松弛过程后会出现由应力释放所致的微弯曲，原理如图5所示。这种微弯曲的纤维与真实血管中胶原蛋白的结构类似，可实现区域I的功效。由该装置收集的弯曲纤维在旋转轴4-1缠绕堆砌成管后，将具备真实血管的非线性力学特性。
用材料拉伸性能测试仪进行血管抗张强度测试及扫描电镜观测，扫描电镜观测可以确定纤维发生了弯曲，拉伸测试结果表明改良后组织工程血管出现了非线性力学区域。如图6所示，改良后组织工程血管出现了非线性力学区域，如图7所示，该特点与真实血管的力学特性高度吻合。
具体实施例：配置10％浓度聚乳酸(PLA)纺丝液利用本例装置进行接收，旋转轴和移动轴之间距离为3cm，移动轴在水平面内运动的周期为2秒、振幅为1.5cm，旋转轴的角速度为2rad/s。最终所制备的血管的宏观形貌如图8所示，微观形貌及力学性能分别如图6和图7所示。
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

Claims (3)

1.一种具有非线性力学性能的静电纺丝组织工程血管的接收装置，包括推进器(2)，推进器(2)与喷丝口(3)相连，喷丝口(3)处设有静电高压施加装置(1)，其特征在于：喷丝口(3)处设有收集装置(4)，收集装置(4)包括旋转轴(4-1)和在水平面内往复运动的移动轴(4-2)。
2.根据权利要求1所述的具有非线性力学性能的静电纺丝组织工程血管的接收装置，其特征在于是按照下述方式进行的：推进器(2)将聚合物溶液输送到喷丝口(3)处，静电高压施加装置(1)在喷丝口(3)处施加静电高压，聚合物溶液在电场斥力的作用下劈裂成纤维，并向收集装置(4)处运动，纤维搭接在旋转轴(4-1)和移动轴(4-2)上，移动轴(4-2)往复运动时，纤维经历拉伸及应力松弛，产生弯曲纤维，弯曲纤维再由旋转轴缠绕堆砌成管。
3.根据权利要求2所述的具有非线性力学性能的静电纺丝组织工程血管的接收装置，其特征在于是按照下述方式进行的：配置10％浓度的聚乳酸纺丝液，推进器(2)将聚乳酸纺丝液输送至静电高压施加装置(1)，旋转轴(4-1)和移动轴(4-2)之间距离为3cm，移动轴(4-2)运动的周期为2秒、振幅为1.5cm，旋转轴(4-1)的角速度为2rad/s。
CN201710309611.8A 2017-05-04 2017-05-04 具有非线性力学性能的静电纺丝组织工程血管的接收装置 Expired - Fee Related CN107201557B (zh)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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