CN107183038A - Plant extracts containing matrine and its applied in pest control - Google Patents
Plant extracts containing matrine and its applied in pest control Download PDFInfo
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- CN107183038A CN107183038A CN201710482815.1A CN201710482815A CN107183038A CN 107183038 A CN107183038 A CN 107183038A CN 201710482815 A CN201710482815 A CN 201710482815A CN 107183038 A CN107183038 A CN 107183038A
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- oil
- plant extracts
- benzoate
- emamectin
- nitenpyram
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention relates to technical field of pesticide combination, specifically disclose a kind of plant extracts containing matrine and its applied in pest control.Raw material containing matrine and other Chinese medicines are crushed in input extractor, then using 20~80% ethanol solutions as Extraction solvent, by 1:3~1:20 solid-liquid ratio, which is put into, soaks 4~8h in tank, Extracting temperature is 40~80 DEG C, and reflux extracting time is 2~12h, and extraction time is 1~6 time, and collection filtrate is concentrated under reduced pressure into the 35~45% of traditional Chinese medicinal material raw materials quality at 50~80 DEG C and produces the plant extracts.Experimental research find that, in the compounding of the plant extracts and chemical active ingredient, plant extracts is significantly increased its active effect to above-mentioned chemical active ingredient, it is wherein the most notable with Nitenpyram, emamectin benzoate or its salt interworking synergistic effect, with the co-toxicity coefficient of emamectin-benzoate up to 120%~1020%, with the co-toxicity coefficient of Nitenpyram up to 120%~372%.
Description
Technical field
The present invention relates to technical field of pesticide combination, specifically, be related to a kind of plant extracts containing matrine and
It is applied in pest control.
Background technology
Pesticide control is the important means of plant protection service, its advantage be it is simple, conveniently, instant effect, as long as medication
Accurately, method is proper with regard to that can obtain remarkable result, to some multiple, fulminant disease pest and weeds, can control or be compressed to rapidly
Under economic indicator, it is ensured that plant growth, increasing both production and income is obtained.
Use the shortcoming of agricultural chemicals：Pesticide dosage is increasing, and number of times is more and more, or even in the warp such as vegetables, fruit tree, tealeaves
High poison, banned pesticides are applied on Ji crop, cause agricultural chemicals to waste, cost increases, natural enemy is reduced, pest resistance strengthens, pollution ring
Border and agricultural product, cause people, animal to be poisoned and dead.
On the premise of crops, ornamental plant, Chinese herbal medicine production of crude drugs is ensured, agricultural chemicals isoreactivity composition input ring is reduced
In border, protect aquatic animal, soil, food, water source etc. not to come to harm or pollute, how to solve this problem extremely urgent.
Central rural work meeting in 2015, central No. 1 file and National Agricultural working conference spirit, close around " steady
Grain increases income tune structure, and upgrading synergy turns mode " main line of work, carry forward vigorously Reducing amount of chemical fertilizer applied and put forward effect, pesticide reduction pest controlling, actively
Efficient output, product safety, resources conservation, the road of environment-friendly modern agricultural development are explored, the Ministry of Agriculture formulates《To 2020
Year fertilizer application amount zero growth rate action scheme》With《To the year two thousand twenty Pesticide use amount zero growth rate action scheme》, in response to agricultural
The policy in portion is called, and applicant is in the case of normal agricultural production is ensured, to improving chemical insecticide, bactericide, nematode killing agent
Effect, reach reduce Environmental Pesticide input amount largely grope, find some plant extracts or plants essential oil
To the synergistic effect of chemical pesticide significantly, it can substantially reduce in chemical noxious composition input environment, protection animal, human and environment
Safety.
CN200510007412.9 discloses a kind of Pesticidal combination containing emamectin benzoate and matrine, but
In the composition of the matrine and emamectin benzoate, matrine is relatively low to the synergistic effect of first ammonia AVM, described to match somebody with somebody
The solvent and emulsifying agent that side is used are harmful to auxiliary agent and drafted in the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC in agricultural chemicals is related to 9 kinds of disablings and 75 kinds of limits
《Insecticides adjuvant, which is limited, uses list》In, it is impossible to meet the demand in present market.
The content of the invention
In order to solve problems of the prior art, carried it is an object of the invention to provide a kind of plant containing matrine
Take thing and its applied in pest control.
In a first aspect, the invention provides a kind of plant extracts containing matrine, its preparation method is：Hardship will be contained
Join alkali raw material 1 and other Chinese medicines constitute raw material 2 crush, input extractor in, then using 20~80% ethanol solutions as
Extraction solvent, by 1:3~1:4~8h is soaked in 20 solid-liquid ratio input tank, Extracting temperature is 40~80 DEG C, reflux extracting time
For 2~12h, extraction time is 1~6 time, collect filtrate be concentrated under reduced pressure at 50~80 DEG C traditional Chinese medicinal material raw materials quality 35~
45% produces.
Wherein, the raw material 1 be selected from kuh-seng, Sophora alopecuroide, root of subprostrate sophora, Sophora viciifolia, sandliving sophora seed, sophora subprostrata, leafy sophora tonkinensis Gapnep,
One or more in the root of Chinese scholar tree, stem, leaf, fruit；The raw material 2 be selected from eucalyptus, the tuber of stemona, Cynanchun komarovii, China tree, arbor-vitae,
In the Chinese herbal medicines such as macleaya cordata, black false hellebore, giant knotweed, frutus cnidii, Chinese cassia tree, grape pip, Chinese ilex, lemongrass, Guan Zhong, calamus, the root of langdu, wild marjoram
It is one or more.
The amount ratio of the raw material 1 and raw material 2 is 5~8:2~5.
The outward appearance of the plant extracts is dark brown thick liquid, and pH value is 3~7, and density is 1.0-1.1g/mL, bitter
It is 0.1%~50% to join alkali content.The discharging of the material after mother liquor is extracted to dry, after the bitter substance in raw material is removed, has been
Local sheep, the feed of ox；Alcohol recycling after concentration, non-environmental-pollution thing is produced, environmentally friendly.
The quality control of plant extracts of the present invention, using matrine content as quality control standard, detection method of content is
Gas chromatography, content reaches that certain index is as qualified.
Wherein matrine content uses liquid chromatogram external standard method, and UV-detector, mobile phase are methanol+water (0.2% phosphoric acid
Hydrogen dipotassium)=80+20 (v/v), C18Kromasil posts, UV-detector is in 220nm measure matrine contents.
In the embodiment of the present invention, the plant extracts is by Sophora alopecuroide and cloves, the tuber of stemona, Cynanchum Komarovii
Grass, Chinese cassia tree, the root of langdu, neem fruit, neem fruit, wild marjoram mixing, are prepared through above-mentioned preparation method.Wherein, the quality of Sophora alopecuroide
Account for 50-80%, cloves, the tuber of stemona, Cynanchun komarovii, Chinese cassia tree, the gross mass of the root of langdu and neem fruit account for 20-50%, cloves, the tuber of stemona,
Cynanchun komarovii, Chinese cassia tree, the root of langdu, neem fruit and the consumption from each other of wild marjoram are suitable.
The present invention still further provides application of the plant extracts in chemical active ingredient drug effect is improved, described
Chemical active ingredient is selected from the acyls such as chrysanthemum esters, the health such as organic phosphates, the effective cypermethrins such as nicotinoids, the phoxim such as Acetamiprid be wide
The microbiology class such as the neires toxins such as the carbamates such as amine, carbosulfan, Cupric sulfate, emamectin benzoate, go out
Insect growth regulator, IGR class, pyrethrum, rotenone, Osthole, the nicotine such as the benzoyl ureas such as young urea, suppression food hydrazine etc.
Natural plants source pesticide.
Experimental research find that, in the compounding of the plant extracts and chemical active ingredient, plant extracts is to upper
Chemical active ingredient is stated to be significantly increased its active effect, wherein with Nitenpyram, emamectin benzoate or its salt interworking
Synergistic effect is the most notable, and the co-toxicity coefficient with emamectin-benzoate is up to 120%~1020%, with Nitenpyram
Co-toxicity coefficient up to 120%~372%.
In order to reduce consumption of the chemical active ingredient in pest control, the present invention to plant extracts and chemism into
The proportioning divided further is probed into.
Research is found, when the weight ratio of the plant extracts and emamectin-benzoate is 50:1~1:50
When, with significant interworking synergistic effect；When weight ratio is 20:1~1:When 10, co-toxicity coefficient is significantly improved；When weight ratio is
10:When 1, co-toxicity coefficient is up to 1020%.
When the weight ratio of the plant extracts and Nitenpyram is 8:1~1:When 8, with significant interworking synergy effect
Really；When weight ratio is 2:1~1:When 2, co-toxicity coefficient is significantly improved；When weight ratio is 1:When 1, co-toxicity coefficient is up to 372%.
Second aspect, based on the foregoing research to plant extracts of the present invention, present invention also offers a kind of Insecticidal combinations
Thing, its active ingredient includes plant extracts of the present invention and emamectin-benzoate or Nitenpyram.
Plant extracts in plant extracts of the present invention and the mutual timing of emamectin-benzoate, Pesticidal combination
Content be 0.1%~50%, the content of emamectin-benzoate is 0.1~70%.
The content of plant extracts is 0.1%- in plant extracts of the present invention and the mutual timing of Nitenpyram, Pesticidal combination
50%, the content of Nitenpyram is 0.1-90%.
The formulation of the Pesticidal combination be missible oil, microemulsion, aqueous emulsion, aqua, super-low capacity spraying finish, pulvis, can
WP, dispersibility granula or granule.
The Pesticidal combination in the preparation mixes plant extracts with chemical active ingredient by a certain percentage, adds molten
Agent or decentralized medium, emulsifying agent, dispersant, water, which are uniformly mixed, is fabricated to corresponding stabilization formulations, is workable make
Agent product.
Solvent used in formulated is one in vegetable oil, plants essential oil, mineral oil, ethanol, glycerine, water in the present invention
Plant or numerous compositions, environmentally safe or degradable solvent.
Wherein：Vegetable oil has soybean oil, peanut oil, sesame oil, palm oil, camellia oil, rapeseed oil, sunflower oil, corn
Oil, olive oil etc..Plants essential oil has grape-kernel oil, cinnamon oil, wintergreen, eucalyptus oil, tea oil, thyme oil, caryophyllus oil, purple
Revive oil, evening primrose oil, raspberry seed oil, pumpkin seed oil, jojoba oil, blackcurrant oil, paniculate swallowwort oil, wheat-germ oil, walnut oil,
Pineapple sarcocarp oil, castor oil, oriental arborvitae seed oil, pomegranate oil, rattletop oil, lichee seed oil, Radish seed oil, Rosa Damascana, safflower oil, frankincense
Oil, perilla herb oil, pot marigold oil, Blumea oil, pelargonium oil, tuber of dwarf lilyturf oil, mace oil, lemon oil, mandarin oil, origanum oil, the stem of noble dendrobium
Oil, grape-fruit seed oil, persic oil, fructus cannabis oil, the fruit of a cubeb litsea tree have, Fructus Aurantii oil, lonicera oil, citronella oil, oil of ginger, parsley oil, sweet basil
Oil, cypress oil san-mou oil, peppermint oil, bletilla striata oil, nutgrass galingale oil, corydalis tuber oil, oil of chamomile, gardenia flower oil, lavender oil, sea-buckthorn
Oil, Java Cananga Oil, rosemary oil, cedar wood oil, jasmine oil, magnolia vine fruit oil, bay-oil, turmeric oil, lily oil, cattail pollen oil, oreodaphene,
Catnip oil, Zanthoxylum essential oil, carrot seed oil, Michelia oil, rue oil, bitter orange oil, styrax oil, ziran oil, tsaoko oil, hops
Oil, sophora flower oil, Citrus aurantium L. var. amara Engl. Oil, all sorts of flowers snakeweed oil, bark of official magnolia oil, Herba Eupatorii Fortunei oil, acacia oil, Eucommia Oil, Sunset Abelmoschus Root oil, Fructus Corni meat
Oil, centella oil, cananga oil, field pennycress oil, turpentine oil, phellandrene oil, linseed oil, aloe, chilli oil, You Jiali
Oil, cassia oil, pinke needle oil, crab oil, orange oil, fenugreek oil, borage oil, camphorated oil, oil ben, selinum oil, safranine
Caul-fat, cajeput oil, passion fruit oil, oil of Herba Artemisiae Scopariae, aniseed oil, rose hip oil, citronella oil, fringed pink oil, bergamot oil, cottonrose hibiscus leaf
Oil, orange oil, corydlis bungeana oil, Artemisia oil, Herba Eupatorii Fortunei oil, sage oil, chinaroot greenbrier oil, coriander oil etc..Mineral sources solvent has mineral oil
And diesel oil.
Emulsifying agent or dispersant used are castor oil polyoxyethylene ether class, fatty alcohol polyethenoxy ether class, polyoxyethylene mountain
Pears alcohol acid anhydride oleic acid esters, calcium dodecyl benzene sulfonate/sodium, lauryl sodium sulfate, dodecyl sodium sulfonate calcium/sodium, sulfomethylated lignin
Sour sodium, polycarboxylate etc., these emulsifying agents are degradable, are not exist《Insecticides adjuvant, which is limited, uses list》In, it is environmentally safe.
Wherein decentralized medium is diatomite, kaolin, cornstarch, cyclodextrin, white carbon, talcum powder etc., is derived from
Natural environment.
Further, the present invention provides following optional formula：
(1) emulsifiable concentrate formulation：
(2) water emulsion formula：
(3) micro-emulsion formulation：
(4) aqua formula：
(5) ultra-low volume oil formula：
(6) wettable powder agent prescription：
(7) aqua formula：
The present invention relates to raw material or reagent be ordinary commercial products, the operation being related to is unless otherwise specified
This area routine operation.
On the basis of common sense in the field is met, above-mentioned each optimum condition can be mutually combined, obtain specific embodiment party
Formula.
The beneficial effects of the present invention are：
The present invention provides a Plant Extracts, and utilizes the work which raises chemical active ingredient in pest control
Property, while prevention effect is ensured, the significant input amount for reducing chemical insecticide, reduction agricultural chemicals is to soil, water body, beneficial
The method of biohazard.
The present invention substitutes hazardous solvent using vegetable oil, plants essential oil, mineral oil, reduces the side that hazardous solvent puts into environment
Method.
The invention provides a kind of Pesticidal products for improving chemical active ingredient activity, reduce environmental contaminants input amount
Preparation method and use technology.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area
Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material, reagent used etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1
Emulsifiable concentrate formulation：
Above-mentioned raw materials are well mixed stabilization formulations are made.
Embodiment 2
Water emulsion formula：
Above-mentioned raw materials are well mixed stabilization formulations are made.
Embodiment 3
Micro-emulsion formulation：
Above-mentioned raw materials are well mixed stabilization formulations are made.
Embodiment 4
Aqua is formulated：
Above-mentioned raw materials are well mixed stabilization formulations are made.
Embodiment 5
Ultra-low volume oil formula：
Above-mentioned raw materials are well mixed stabilization formulations are made.
Embodiment 6
Wettable powder agent prescription：
Above-mentioned raw materials are well mixed stabilization formulations are made.
Embodiment 7
Aqua is formulated：
Above-mentioned raw materials are well mixed stabilization formulations are made.
The indoor biometricses of experimental example 1 (one)
This experimental example be used for study different quality than plant extracts (matrine 5%) (A) and emamectin benzoate
Benzoate (B) compounds the co-toxicity to diamondback moth.
First, test medicine and test worm
74.6% Methylaminoemamectin benzoate, purchased from Hebei Weiyuan biochemistry Co limited company；
The plant extracts (5% matrine) prepared according to the inventive method；
3 age diamondback moths, field collection, indoor feeding.
2nd, assay method
Chinese cabbage is cut into 5cm*5cm small pieces, in the culture dish for being put into preparation 12.5cm, filter paper is lined with ware.By dish leaf
Soak, take out immediately in the decoction diluted, dry, then add in culture dish, 10 larvas are connected on blade face.
Three repetitions, if clear water is compareed.1d, 3d, 7d check death state, then statistical disposition after processing.
3rd, virulence evaluation method
Virulence, which is judged, uses the abundant methods of Sun Yun, calculates co-toxicity coefficient method (co-toxicity coefficient, CTC).
4th, test result
The plant extracts of table 1 (matrine 5%) and emamectin-benzoate different ratio are to diamondback moth
The virulence result of (Plutella xylostella) 3 instar larvaes (48h)
As can be seen from the table, the compounding of plant extracts (matrine 5%) and emamectin-benzoate increases
Effect is notable, spends coefficient together up to 1020.81.
The indoor biometricses of experimental example 2 (two)
This experimental example be used to studying different quality than plant extracts (matrine 5%) (A) and Nitenpyram (B) compound
To the co-toxicity of brown paddy plant hopper.
First, test medicine and test worm
95% Nitenpyram active compound, purchased from Shandong United Pesticide Industry Co., Ltd.；
The plant extracts (5% matrine) prepared according to the inventive method；
Brown paddy plant hopper, the collection of tritonymph field, indoor feeding.
Two assay methods
By the small pieces of paddy rice 5cm length, in the culture dish for being put into preparation 12.5cm, filter paper is lined with ware.By blade in dilution
Soaked in good decoction, take out, dry immediately, then added in culture dish, 10 larvas are connected on blade face.Three weights
It is multiple, if clear water is compareed.1d, 3d, 7d check death state, then statistical disposition after processing.
3rd, virulence evaluation method
Virulence, which is judged, uses the abundant methods of Sun Yun, calculates co-toxicity coefficient method (co-toxicity coefficient, CTC).
4th, test result
The plant extracts of table 2 (matrine 5%) and Nitenpyram different ratio are to brown paddy plant hopper (Nilaparvata
Lugens) the virulence result of 3 age nymphs (48h)
As can be seen from the table, the compound synergic of plant extracts (matrine 5%) and Nitenpyram is notable.Two kinds of materials
Synergy is optimal when concentration is suitable, spends coefficient together and is up to 372.28.
The field control effectiveness test of experimental example 3 (one)
First, test medicine
Test medicine 1：The plant extracts of 0.5% emamectin-benzoate+10% (matrine 5%)
Test medicine 2：The aqueous matrine solution of 0.5% emamectin-benzoate+0.5%
Wherein, 5% kuh-seng alkali liquor, commercially available.
Test medicine 3：1% emamectin-benzoate missible oil, purchased from Shandong Gui He bio tech ltd.
2nd, trial crops and controlling object
1st, trial crops：Wild cabbage.
2nd, controlling object：Three age diamondback moths.
3rd, experimental design
Using experiment great Qu methods of comparison, repetition is not set.
4th, experimental field basic condition
Experiment is carried out in Shandong Guan County Xie Dianxiang Qian She village village cabbage field, and long-term planting vegetable, physical features is flat, irrigation and drainage side
Just, soil property sandy loam, the content of organic matter 2.22%, pH value 7.1, all experimental plot tillage and cultivations are consistent during experiment, and
It is consistent with local agricultural practice (GAP).
Dispenser when 24 days 16 June, dispenser period is diamondback moth early period of origination, and temperature is 28 DEG C, relative humidity during dispenser
70%, cloudy turn to fine, 2~3 grades of same day wind-force, completes investigation within 7 days after spray.
5th, dispenser and preventive effect investigation
1st, dispenser：During 24 days 16 June, using conventional spraying, in the disposable dispenser of diamondback moth low instar larvae phase, Linyi City is used
Strange brave 3WBS -16D type knapsack hand sprayer even sprayings, fluid volume is 30kg/ mus.
2nd, investigation method：Using fixed five point sampling, 4 plants of wild cabbages are investigated per sampling point.Investigated before medicine after insect population, medicine
1st, 3, investigation remaining insect population number during 7 day afternoon 17 or so.Insect population reaches more than 55.
3rd, drug effect computational methods
6th, result and analysis
Plant extracts (matrine) aqua of 3 0.5% emamectin-benzoate of table+10% is to wild cabbage pickles
The preventive effect of moth
As a result show, 5% kuh-seng alkali liquor purchased in market is added in emamectin benzoate or plant of the invention carries
Take after thing, the plant extraction liquid in 5% kuh-seng alkali liquor and the present invention can improve the effect of emamectin-benzoate
Really, it is consistent with patent CN200510007412.9.
In the extract of 0.5% emamectin-benzoate+10% (5% matrine) aqua and 1% methylamino Ah
When dimension rhzomorph benzoate missible oil usage amount is half, (5% is bitter for the extract of 0.5% emamectin-benzoate+10%
Join alkali) aqua effect it is more preferable.When dosage is 1/4, the prevention effect to diamondback moth is suitable, emamectin-benzoate
Input amount there was only only the 25% of comparison medicament, significantly reduce the input amount of chemical pesticide.
Used in the active ingredient of the extract of 0.5% emamectin-benzoate+10% (5% matrine) aqua
When amount is the half of the aqueous matrine solution of 0.5% emamectin-benzoate+0.5%, prevention effect is suitable.Carried with 10%
Take after thing interworking, the synergy of emamectin benzoate is more notable.
Therefore, plant extracts (5% matrine) of the present invention is better than 5% kuh-seng alkali liquor purchased in market.The present invention
The plant extracts (5% matrine) of offer has significant economic value.The chemical active ingredient put into environment is reduced
75%, significantly protect environment.
The field control effectiveness test of experimental example 4 (two)
First, test medicine
Test medicine 1：10% Nitenpyram aqua, is produced purchased from Zhejiang Sega Science and Technology Co., Ltd..
Test medicine 2：The plant extracts of 10% Nitenpyram+10% (matrine 5%)
2nd, trial crops and controlling object
1st, trial crops：Paddy rice.
2nd, controlling object：Brown paddy plant hopper.
3rd, experimental design
Using experiment great Qu methods of comparison, repetition is not set.
4th, experimental field basic condition
Experiment is carried out in the Chinese Academy of Agricultural Sciences of Beijing paddy rice, and long-term planting vegetable, physical features is flat, and irrigation and drainage are convenient,
Soil property sandy loam, the content of organic matter 2.37%, pH value 6.5, all experimental plot tillage and cultivations are consistent during experiment, and and work as
Ground agricultural practice (GAP) is consistent.
Dispenser when 24 days 16 June, dispenser period is diamondback moth early period of origination, and temperature is 35 DEG C, relative humidity during dispenser
80%, cloudy turn to fine, 2~3 grades of same day wind-force, completes investigation within 7 days after spray.
5th, dispenser and preventive effect investigation
1st, dispenser：During 24 days 16 June, using conventional spraying, in the disposable dispenser of diamondback moth low instar larvae phase, with 3WBS-
16D type knapsack hand sprayer even sprayings, fluid volume is 30kg/ mus.
2nd, investigation method：Using fixed five point sampling, 5 plants of paddy rice are investigated per sampling point.Investigated before medicine after insect population, medicine
1st, 3, investigation remaining insect population number during 7 day afternoon 17 or so.Insect population reaches more than 55.
3rd, drug effect computational methods
6th, result and analysis
Preventive effect of the plant extracts of 4 10% Nitenpyram of table+10% (5% matrine) aqua to Brown Planthopper
As a result show, in the Nitenpyram of identical active ingredient, add plant extracts prevention effect high 20% with
On.During by active component Nitenpyram reduction by 50%, effect is higher than the Nitenpyram aqua of comparison medicament 10%, and active component is entered
Entering the input amount of environment reduces 50%, significantly protects environment.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of plant extracts containing matrine, it is characterised in that its preparation method is：By the raw material 1 containing matrine
The raw material 2 constituted with other Chinese medicines is crushed, in input extractor, then 20~80% ethanol solutions are pressed as Extraction solvent
1:3~1:4~8h is soaked in 20 solid-liquid ratio input tank, Extracting temperature is 40~80 DEG C, and reflux extracting time is 2~12h, is carried
It is 1~6 time to take number of times, and collection filtrate is concentrated under reduced pressure into the 35~45% of traditional Chinese medicinal material raw materials quality at 50~80 DEG C and produced.
2. plant extracts according to claim 1, it is characterised in that the raw material 1 is selected from kuh-seng, Sophora alopecuroide, wide beans
One or more in root, Sophora viciifolia, sandliving sophora seed, sophora subprostrata, leafy sophora tonkinensis Gapnep, the root of Chinese scholar tree, stem, leaf, fruit；The raw material 2
Selected from eucalyptus, the tuber of stemona, Cynanchun komarovii, China tree, arbor-vitae, macleaya cordata, black false hellebore, giant knotweed, frutus cnidii, Chinese cassia tree, grape pip, Chinese ilex,
One or more in the Chinese herbal medicines such as lemongrass, Guan Zhong, calamus, the root of langdu, wild marjoram.
3. plant extracts according to claim 2, it is characterised in that the amount ratio of the raw material 1 and raw material 2 for 5~
8:2~5.
4. application of the plant extracts in chemical active ingredient drug effect is improved described in any one of claims 1 to 3, its feature
It is, the chemical active ingredient is selected from the chrysanthemum esters such as organic phosphates, the effective cypermethrins such as nicotinoids, the phoxims such as Acetamiprid
Neires toxin, the emamectin benzoate such as carbamates, the Cupric sulfate such as amide-type, carbosulfan etc. such as class, health be wide
Insect growth regulator, IGR class, pyrethrum, rotenone, the cnidium monnieris such as the benzoyl ureas such as microbiology class, d ichlorbenzuron, suppression food hydrazine
The natural plants source pesticides such as sub- element, nicotine.
5. application according to claim 4, it is characterised in that the chemical active ingredient is emamectin benzoate benzene first
Hydrochlorate, the weight ratio of the plant extracts and emamectin-benzoate is 50:1~1:50, preferably 20:1~
1:10, more preferably 10:1.
6. application according to claim 4, it is characterised in that the chemical active ingredient is Nitenpyram, the plant
The weight ratio of extract and Nitenpyram is 8:1~1:8, preferably 2:1~1:2, more preferably 1:1.
7. a kind of Pesticidal combination, it is characterised in that the plant that its active ingredient includes described in any one of claims 1 to 3 carries
Take thing and emamectin-benzoate or Nitenpyram.
8. Pesticidal combination according to claim 7, it is characterised in that the plant extracts and emamectin benzoate
The weight ratio of benzoate is 50:1~1:50, preferably 20:1~1:10, more preferably 10:1.
9. Pesticidal combination according to claim 7, it is characterised in that the weight of the plant extracts and Nitenpyram
Than for 8:1~1:8, preferably 2:1~1:2, more preferably 1:1.
10. the Pesticidal combination according to any one of claim 7~9, it is characterised in that the formulation of the Pesticidal combination
For missible oil, microemulsion, aqueous emulsion, aqua, super-low capacity spraying finish, pulvis, wettable powder, dispersibility granula or particle
Agent.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107751200A (en) * | 2017-11-29 | 2018-03-06 | 广东中迅农科股份有限公司 | A kind of environmentally friendly insecticide using garland chrysanthemum vegetarian as synergist |
| CN107787757A (en) * | 2017-11-24 | 2018-03-13 | 桂林国农生态农业有限公司 | A kind of high-yield planting method of gingko |
| CN108887284A (en) * | 2018-07-12 | 2018-11-27 | 中国农业科学院棉花研究所 | A kind of Pesticidal combination containing imidaclothiz and matrine and its application |
| CN109997632A (en) * | 2019-03-21 | 2019-07-12 | 广西壮族自治区农业科学院水稻研究所 | The implantation methods of green selenium-rich rice |
| CN110279816A (en) * | 2019-06-27 | 2019-09-27 | 张加勇 | The low stimulation of one kind external insecticide for animals and preparation method thereof |
| CN113349226A (en) * | 2021-05-26 | 2021-09-07 | 仲恺农业工程学院 | Application of rose oil in pest control |
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| CN1650710A (en) * | 2005-02-18 | 2005-08-10 | 李建中 | Insecticide composition containing methylamino Avermectin and matrine |
| CN102302018A (en) * | 2011-07-16 | 2012-01-04 | 海利尔药业集团股份有限公司 | Insecticidal composition including nitenpyram and matrine |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1650710A (en) * | 2005-02-18 | 2005-08-10 | 李建中 | Insecticide composition containing methylamino Avermectin and matrine |
| CN102302018A (en) * | 2011-07-16 | 2012-01-04 | 海利尔药业集团股份有限公司 | Insecticidal composition including nitenpyram and matrine |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107787757A (en) * | 2017-11-24 | 2018-03-13 | 桂林国农生态农业有限公司 | A kind of high-yield planting method of gingko |
| CN107751200A (en) * | 2017-11-29 | 2018-03-06 | 广东中迅农科股份有限公司 | A kind of environmentally friendly insecticide using garland chrysanthemum vegetarian as synergist |
| CN108887284A (en) * | 2018-07-12 | 2018-11-27 | 中国农业科学院棉花研究所 | A kind of Pesticidal combination containing imidaclothiz and matrine and its application |
| CN109997632A (en) * | 2019-03-21 | 2019-07-12 | 广西壮族自治区农业科学院水稻研究所 | The implantation methods of green selenium-rich rice |
| CN110279816A (en) * | 2019-06-27 | 2019-09-27 | 张加勇 | The low stimulation of one kind external insecticide for animals and preparation method thereof |
| CN113349226A (en) * | 2021-05-26 | 2021-09-07 | 仲恺农业工程学院 | Application of rose oil in pest control |
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