CN107182785A - Strawberry tissue culture method - Google Patents
Strawberry tissue culture method Download PDFInfo
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- CN107182785A CN107182785A CN201710456571.XA CN201710456571A CN107182785A CN 107182785 A CN107182785 A CN 107182785A CN 201710456571 A CN201710456571 A CN 201710456571A CN 107182785 A CN107182785 A CN 107182785A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention relates to Strawberry tissue culture method, comprise the following steps：Strawberry explant is taken, successively with alcohol, bromogeramine, mercury chloride and peracetic acid disinfectant；Adventitious bud induction culture is carried out to strawberry explant using the first culture medium；Shoot proliferation culture, the plant taken root are carried out to strawberry explant using the second culture medium；Plant hardening domestication to taking root, is then soaked with carbendazol wettable powder, then moves into culture in the 3rd culture medium, obtains strawberry.The Strawberry tissue culture method of the present invention, its survival rate to explant processing is up to 75%, improves 40 percentage points than routine disinfection processing formulation, its fruiting rate is up to more than 95%, and well developed root system, and seedling endures that leaf is green, and transplanted seedling quality is good.The obtained strawberry of Strawberry tissue culture method of the present invention is healthy and strong, disease resistance is strong, yield is high, and obtained strawberry is fragrant and sweet strong, in good taste, and sour-sweet moderate, quality is splendid, resistance to accumulating, with high promotional value.
Description
Technical field
The present invention relates to a kind of Strawberry tissue culture method.
Background technology
Strawberry be called the foreign certain kind of berries, the certain kind of berries etc., the category rose family, Fragaria, herbaceos perennial.Strawberry fruit is delicious red tender, fruit
Meat succulence, containing special strong fruit aroma, rich in vitamin C, vitamin A, carrotene etc., there is higher nutritive value
And medical value.
But, Strawberry Virus has a strong impact on the yield of strawberry, generally results in underproduction 30%-50%, strawberry is infected at present
Virosis, mainly have four kinds, i.e. Strawberry mottle virus (strawberry mottle virus), strawberry crinkle virus
(strawberry crinkle virus), strawberry be light and malicious (the strawberry mild-yellow edge of yellow edge
Virus), strawberry veinbanding virus (strawberry vein-band virus).It is using tissue cultures and virus detection techniques
The effective way of virosis is prevented and treated, the main skill of the new stock breeding promotion rate of strawberry and Virus-free seedcane production is also to speed up
Art means.But, the virus-free strawberry seedling stolon reproduction technique code and production technology regulation of complete set are not yet set up at present.
The content of the invention
It is an object of the present invention to propose a kind of Strawberry tissue culture method.
The Strawberry tissue culture method of the present invention, comprises the following steps：S101：Strawberry explant is taken, at 45 DEG C~55 DEG C
At a temperature of be heat-treated 3min~8min, then successively with mass concentration for 70%~80% alcohol disinfecting 25s~35s, use matter
Bromogeramine sterilization 10min~30min that amount concentration is 0.1%~1%, with mass concentration be 0.01%~1% mercury chloride and
Mass concentration is 0.1%~1% peracetic acid disinfectant 2min~10min；S102：Using the first culture medium to the step
Strawberry explant treated S101 carries out adventitious bud induction culture 28 days~32 days at a temperature of 15 DEG C~30 DEG C；S103：Adopt
Shoot proliferation training is carried out at a temperature of 20 DEG C~30 DEG C to the step S102 strawberry explants treated with the second culture medium
Support 28 days~32 days, the plant taken root；S104：The plant hardening taken root is tamed 1 day~3 days, quality is then used
Concentration soaks 25min~35min for 40%~60% carbendazol wettable powder, then moves into the 3rd culture medium, at 20 DEG C
Cultivated 50 days~60 days at a temperature of~30 DEG C, obtain strawberry.
The Strawberry tissue culture method of the present invention, its survival rate handled explant is up to 75%, than routine disinfection place
Manage formulation and improve 40 percentage points, its fruiting rate is up to more than 95%, and well developed root system, seedling endures that leaf is green, and transplanted seedling quality is good.This
Strawberry that the Strawberry tissue culture method of invention is obtained is healthy and strong, disease resistance is strong, yield is high, and obtained strawberry is fragrant and sweet strong,
In good taste, sour-sweet moderate, quality is splendid, resistance to accumulating, with high promotional value.
In addition, Strawberry tissue culture method according to the above embodiment of the present invention, can also have technology additional as follows
Feature：
Further, first culture medium includes the parts by weight of parts by weight of MS culture mediums 50~60, mass concentration and is
The parts by weight of 1.0mg/L parts by weight of 6- benzyls aminoadenine 1~10, mass concentration for 0.1mg/L the parts by weight of methyl α-naphthyl acetate 0.1~
The parts by weight of 1 parts by weight, the parts by weight of white sugar 1~10 and mass concentration for 3.8g/L the parts by weight of parts by weight of agar 1~10.
Further, second culture medium includes the parts by weight of parts by weight of MS culture mediums 50~60, mass concentration and is
The parts by weight of the parts by weight of 0.01mg/L parts by weight of methyl α-naphthyl acetate 0.1~1, the parts by weight of white sugar 1~10 and mass concentration are 3.8g/L's
The parts by weight of parts by weight of agar 1~10.
Further, it is 1 that the 3rd culture medium, which includes weight ratio,:1 broad-leaved fertile soil and the dregs of a decoction, the dregs of a decoction bag
Include：30 parts~50 parts of ginkgo leaf, 1 part~10 parts of radix scutellariae, 1 part~10 parts of glossy privet seed, 0.1 part~1 part of cordate houttuynia, polygonum capitatum 0.1
Part~1 part and 0.1 part~1 part of sealwort.Wherein, the dregs of a decoction are the remaining discarded objects after traditional Chinese medicine extraction, are applied in group
It is to turn waste into wealth to train true in transplantation of seedlings matrix, energy-saving and environment-friendly.
Further, in the step S104, blade face is sprayed when being cultivated the 14th day~17 days in the 3rd culture medium
The potassium dihydrogen phosphate that mass concentration is 0.01%~1% and the urea that mass concentration is 0.01%~1% are applied, hereafter, weekly two
The urea that the potassium dihydrogen phosphate and mass concentration that secondary foliage-spray mass concentration is 0.01%~1% are 0.01%~1%.
Further, in the step S104, when being cultivated in the 3rd culture medium, every 10 days~15 days to institute
State the plant taken root to be watered, and add in water carbendazim；Wherein, the weight ratio of the water and the carbendazim is
1000：1.
Further, in the step S104, quality is sprayed when being cultivated the 7th day~10 days in the 3rd culture medium
Concentration is the nutrient solution of 50% MS culture mediums.
Further, in the step S101, the strawberry explant is the stolon of strawberry.
It is another object of the present invention to the strawberry for proposing that described Strawberry tissue culture method is obtained.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
Embodiments of the invention are described below in detail, the embodiment is exemplary, it is intended to for explaining the present invention, and
It is not considered as limiting the invention.
Embodiment 1
Embodiment 1 proposes a kind of Strawberry tissue culture method, comprises the following steps：
(1) stolon of strawberry is taken, 8min is heat-treated at a temperature of 45 DEG C, then uses mass concentration to be 70% successively
Alcohol disinfecting 35s, be 0.1% with mass concentration bromogeramine sterilization 30min, with mass concentration be 0.01% mercury chloride and
Mass concentration is 1% peracetic acid disinfectant 2min.
(2) the strawberry explant treated using the first culture medium to the step (1) carries out indefinite at a temperature of 30 DEG C
Bud inducement cultivation 28 days；Wherein, first culture medium includes the 6- that the parts by weight of MS culture mediums 60, mass concentration are 1.0mg/L
The parts by weight of methyl α-naphthyl acetate 1, the parts by weight of white sugar 1 and the mass concentration that the parts by weight of benzyl aminoadenine 1, mass concentration are 0.1mg/L be
The 3.8g/L parts by weight of agar 10.
(3) the strawberry explant treated using the second culture medium to the step (2) carries out subculture at a temperature of 20 DEG C
Multiplying culture 32 days, the plant taken root；Wherein, second culture medium includes the parts by weight of MS culture mediums 50, mass concentration
The parts by weight of agar 10 for being 3.8g/L for the 0.01mg/L parts by weight of methyl α-naphthyl acetate 1, the parts by weight of white sugar 1 and mass concentration.
(4) the plant hardening taken root is tamed 1 day, then with the carbendazol wettable powder that mass concentration is 60%
25min is soaked, then is moved into the 3rd culture medium, is cultivated 50 days at a temperature of 30 DEG C, obtains strawberry.3rd culture medium
It is 1 including weight ratio:1 broad-leaved fertile soil and the dregs of a decoction, the dregs of a decoction include：50 parts of ginkgo leaf, 1 part of radix scutellariae, 10 parts of glossy privet seed,
0.1 part of 0.1 part of cordate houttuynia, 1 part of polygonum capitatum and sealwort.Wherein, the dregs of a decoction are the remaining discarded objects after traditional Chinese medicine extraction, by it
It is to turn waste into wealth to apply true on tissue-cultured seedling transplanting medium, energy-saving and environment-friendly.The 10th is cultivated in the 3rd culture medium
It when spray mass concentration be 50% MS culture mediums nutrient solution.Blade face is sprayed when being cultivated the 14th day in the 3rd culture medium
The potassium dihydrogen phosphate that mass concentration is 1% and the urea that mass concentration is 0.01% are applied, hereafter, foliage-spray quality twice a week
The urea that the potassium dihydrogen phosphate and mass concentration that concentration is 1% are 0.01%.When being cultivated in the 3rd culture medium, every 15
It waters to the plant taken root, and adds in water carbendazim；Wherein, the weight ratio of the water and the carbendazim
For 1000：1.
Embodiment 2
Embodiment 2 proposes a kind of Strawberry tissue culture method, comprises the following steps：
(1) stolon of strawberry is taken, 3min is heat-treated at a temperature of 55 DEG C, then uses mass concentration to be 80% successively
Alcohol disinfecting 25s, be 1% with mass concentration bromogeramine sterilization 10min, with mass concentration be 1% mercury chloride and quality
Concentration is 0.1% peracetic acid disinfectant 10min.
(2) the strawberry explant treated using the first culture medium to the step (1) carries out indefinite at a temperature of 15 DEG C
Bud inducement cultivation 32 days；Wherein, first culture medium includes the 6- that the parts by weight of MS culture mediums 50, mass concentration are 1.0mg/L
The parts by weight of benzyl aminoadenine 10, mass concentration are dense for the 0.1mg/L parts by weight of methyl α-naphthyl acetate 0.1, the parts by weight of white sugar 10 and quality
Spend the parts by weight of agar 1 for 3.8g/L.
(3) the strawberry explant treated using the second culture medium to the step (2) carries out subculture at a temperature of 30 DEG C
Multiplying culture 28 days, the plant taken root；Wherein, second culture medium includes the parts by weight of MS culture mediums 60, mass concentration
The parts by weight of agar 1 for being 3.8g/L for the 0.01mg/L parts by weight of methyl α-naphthyl acetate 0.1, the parts by weight of white sugar 10 and mass concentration.
(4) the plant hardening taken root is tamed 3 days, then with the carbendazol wettable powder that mass concentration is 40%
35min is soaked, then is moved into the 3rd culture medium, is cultivated 60 days at a temperature of 20 DEG C, obtains strawberry.3rd culture medium
It is 1 including weight ratio:1 broad-leaved fertile soil and the dregs of a decoction, the dregs of a decoction include：30 parts of ginkgo leaf, 10 parts of radix scutellariae, 1 part of glossy privet seed,
1 part of 1 part of cordate houttuynia, 0.1 part of polygonum capitatum and sealwort.Wherein, the dregs of a decoction are the remaining discarded objects after traditional Chinese medicine extraction, are answered
It is energy-saving and environment-friendly with being really to turn waste into wealth on tissue-cultured seedling transplanting medium.When being cultivated the 7th day in the 3rd culture medium
Spray the nutrient solution for the MS culture mediums that mass concentration is 50%.Foliage-spray matter when being cultivated the 17th day in the 3rd culture medium
Measure the potassium dihydrogen phosphate that concentration is 0.01% and the urea that mass concentration is 1%, hereafter, foliage-spray mass concentration twice a week
The urea that potassium dihydrogen phosphate and mass concentration for 0.01% are 1%.It is right every 10 days when being cultivated in the 3rd culture medium
The plant taken root is watered, and adds in water carbendazim；Wherein, the weight ratio of the water and the carbendazim is
1000：1.
Embodiment 3
Embodiment 3 proposes a kind of Strawberry tissue culture method, comprises the following steps：
(1) stolon of strawberry is taken, 5min is heat-treated at a temperature of 50 DEG C, then uses mass concentration to be 75% successively
Alcohol disinfecting 30s, be 0.5% with mass concentration bromogeramine sterilization 20min, with mass concentration be 0.1% mercury chloride and
Mass concentration is 0.5% peracetic acid disinfectant 6min.
(2) the strawberry explant treated using the first culture medium to the step (1) carries out indefinite at a temperature of 23 DEG C
Bud inducement cultivation 30 days；Wherein, first culture medium includes the 6- that the parts by weight of MS culture mediums 55, mass concentration are 1.0mg/L
The parts by weight of benzyl aminoadenine 5, mass concentration are the 0.1mg/L parts by weight of methyl α-naphthyl acetate 0.5, the parts by weight of white sugar 5 and mass concentration
For the 3.8g/L parts by weight of agar 5.
(3) the strawberry explant treated using the second culture medium to the step (2) carries out subculture at a temperature of 25 DEG C
Multiplying culture 30 days, the plant taken root；Wherein, second culture medium includes the parts by weight of MS culture mediums 55, mass concentration
The parts by weight of agar 5 for being 3.8g/L for the 0.01mg/L parts by weight of methyl α-naphthyl acetate 0.5, the parts by weight of white sugar 5 and mass concentration.
(4) the plant hardening taken root is tamed 2 days, then with the carbendazol wettable powder that mass concentration is 50%
30min is soaked, then is moved into the 3rd culture medium, is cultivated 55 days at a temperature of 25 DEG C, obtains strawberry.3rd culture medium
It is 1 including weight ratio:1 broad-leaved fertile soil and the dregs of a decoction, the dregs of a decoction include：40 parts of ginkgo leaf, 5 parts of radix scutellariae, 5 parts of glossy privet seed,
0.5 part of 0.5 part of cordate houttuynia, 0.5 part of polygonum capitatum and sealwort.Wherein, the dregs of a decoction are the remaining discarded objects after traditional Chinese medicine extraction, will
It is to turn waste into wealth that it, which is applied true on tissue-cultured seedling transplanting medium, energy-saving and environment-friendly.The 8th is cultivated in the 3rd culture medium
It when spray mass concentration be 50% MS culture mediums nutrient solution.Blade face is sprayed when being cultivated the 15th day in the 3rd culture medium
The potassium dihydrogen phosphate that mass concentration is 0.05% and the urea that mass concentration is 0.05% are applied, hereafter, foliage-spray twice a week
The urea that the potassium dihydrogen phosphate and mass concentration that mass concentration is 0.05% are 0.05%.Cultivated in the 3rd culture medium
When, the plant taken root is watered every 12 days, and add in water carbendazim；Wherein, the water and many bacterium
The weight ratio of spirit is 1000：1.
Comparative example 1
Comparative example 1 proposes a kind of Strawberry tissue culture method, comprises the following steps：
(1) stolon of strawberry is taken, adventitious bud induction culture is carried out at a temperature of 23 DEG C 30 days；Wherein, described
It is dense for the 1.0mg/L parts by weight of 6- benzyls aminoadenine 5, quality that one culture medium includes the parts by weight of MS culture mediums 55, mass concentration
The parts by weight of agar 5 that the parts by weight of methyl α-naphthyl acetate 0.5, the parts by weight of white sugar 5 and the mass concentration that degree is 0.1mg/L are 3.8g/L.
(2) the strawberry explant treated using the second culture medium to the step (1) carries out subculture at a temperature of 25 DEG C
Multiplying culture 30 days, the plant taken root；Wherein, second culture medium includes the parts by weight of MS culture mediums 55, mass concentration
The parts by weight of agar 5 for being 3.8g/L for the 0.01mg/L parts by weight of methyl α-naphthyl acetate 0.5, the parts by weight of white sugar 5 and mass concentration.
(3) the plant hardening taken root is tamed 2 days, then with the carbendazol wettable powder that mass concentration is 50%
30min is soaked, then is moved into the 3rd culture medium, is cultivated 55 days at a temperature of 25 DEG C, obtains strawberry.3rd culture medium
It is 1 including weight ratio:1 broad-leaved fertile soil and the dregs of a decoction, the dregs of a decoction include：40 parts of ginkgo leaf, 5 parts of radix scutellariae, 5 parts of glossy privet seed,
0.5 part of 0.5 part of cordate houttuynia, 0.5 part of polygonum capitatum and sealwort.Wherein, the dregs of a decoction are the remaining discarded objects after traditional Chinese medicine extraction, will
It is to turn waste into wealth that it, which is applied true on tissue-cultured seedling transplanting medium, energy-saving and environment-friendly.The 8th is cultivated in the 3rd culture medium
It when spray mass concentration be 50% MS culture mediums nutrient solution.Blade face is sprayed when being cultivated the 15th day in the 3rd culture medium
The potassium dihydrogen phosphate that mass concentration is 0.05% and the urea that mass concentration is 0.05% are applied, hereafter, foliage-spray twice a week
The urea that the potassium dihydrogen phosphate and mass concentration that mass concentration is 0.05% are 0.05%.Cultivated in the 3rd culture medium
When, the plant taken root is watered every 12 days, and add in water carbendazim；Wherein, the water and many bacterium
The weight ratio of spirit is 1000：1.
Comparative example 2
Comparative example 2 proposes a kind of Strawberry tissue culture method, comprises the following steps：
(1) stolon of strawberry is taken, 5min is heat-treated at a temperature of 50 DEG C, then uses mass concentration to be 75% successively
Alcohol disinfecting 30s, be 0.5% with mass concentration bromogeramine sterilization 20min, with mass concentration be 0.1% mercury chloride and
Mass concentration is 0.5% peracetic acid disinfectant 6min.
(2) the strawberry explant treated using the first culture medium to the step (1) carries out indefinite at a temperature of 23 DEG C
Bud inducement cultivation 30 days；Wherein, first culture medium includes the 6- that the parts by weight of MS culture mediums 55, mass concentration are 1.0mg/L
The parts by weight of benzyl aminoadenine 5, mass concentration are the 0.1mg/L parts by weight of methyl α-naphthyl acetate 0.5, the parts by weight of white sugar 5 and mass concentration
For the 3.8g/L parts by weight of agar 5.
(3) the strawberry explant treated using the second culture medium to the step (2) carries out subculture at a temperature of 25 DEG C
Multiplying culture 30 days, the plant taken root；Wherein, second culture medium includes the parts by weight of MS culture mediums 55, mass concentration
The parts by weight of agar 5 for being 3.8g/L for the 0.01mg/L parts by weight of methyl α-naphthyl acetate 0.5, the parts by weight of white sugar 5 and mass concentration.
(4) the plant hardening taken root is tamed 2 days, then with the carbendazol wettable powder that mass concentration is 50%
30min is soaked, then is moved into broad-leaved fertile soil, is cultivated 55 days at a temperature of 25 DEG C, obtains strawberry.In broad-leaved fertile soil
The nutrient solution for the MS culture mediums that mass concentration is 50% is sprayed when cultivating the 8th day.Leaf when being cultivated the 15th day in broad-leaved fertile soil
Face sprays the urea that the potassium dihydrogen phosphate that mass concentration is 0.05% and mass concentration are 0.05%, hereafter, twice a week blade face
Spray the potassium dihydrogen phosphate that mass concentration is 0.05% and the urea that mass concentration is 0.05%.Cultivated in broad-leaved fertile soil
When, the plant taken root is watered every 12 days, and add in water carbendazim；Wherein, the water and many bacterium
The weight ratio of spirit is 1000：1.
The stolon of 250 plants of strawberries is taken at random, is divided into 5 groups, is passed through embodiment 1-3 and comparative example 1-2 respectively
Method plantation, and calculate the survival rate and fruiting rate of embodiment 1-3 and comparative example 1-2 strawberry, wherein, fruiting rate is bears
The plant that survives of strawberry accounts for the ratio for surviving plant.It the results are shown in Table 1.
Table 1：The survival rate and fruiting rate of the stolon of strawberry
| Survival rate/% | Fruiting rate/% | |
| Embodiment 1 | 78 | 98 |
| Embodiment 2 | 75 | 96 |
| Embodiment 3 | 82 | 98 |
| Comparative example 1 | 35 | 76 |
| Comparative example 2 | 32 | 80 |
As it can be seen from table 1 the Strawberry tissue culture method of the present invention, its survival rate handled explant is up to
75%, 40 percentage points are improved than routine disinfection processing formulation, its fruiting rate is up to more than 95%, and well developed root system, Miao Tingye
Green, transplanted seedling quality is good.
The obtained strawberry of Strawberry tissue culture method of the present invention is healthy and strong, disease resistance is strong, yield is high, obtained strawberry
Fragrant and sweet strong, in good taste, sour-sweet moderate, quality is splendid, resistance to accumulating, with high promotional value.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or the spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the not be the same as Example or the feature of example and non-be the same as Example or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (9)
1. a kind of Strawberry tissue culture method, it is characterised in that comprise the following steps：
S101：Strawberry explant is taken, 3min~8min is heat-treated at a temperature of 45 DEG C~55 DEG C, is then with mass concentration successively
70%~80% alcohol disinfecting 25s~35s, with mass concentration for 0.1%~1% bromogeramine sterilize 10min~
30min, with mass concentration be 0.01%~1% mercury chloride and mass concentration be 0.1%~1% peracetic acid disinfectant 2min~
10min；
S102：The step S101 strawberry explants treated are carried out at a temperature of 15 DEG C~30 DEG C using the first culture medium
Adventitious bud induction culture 28 days~32 days；
S103：The step S102 strawberry explants treated are carried out at a temperature of 20 DEG C~30 DEG C using the second culture medium
Shoot proliferation culture 28 days~32 days, the plant taken root；
S104：The plant hardening taken root is tamed 1 day~3 days, then with the carbendazim that mass concentration is 40%~60%
Wettable powder soaks 25min~35min, then moves into the 3rd culture medium, and 50 days~60 are cultivated at a temperature of 20 DEG C~30 DEG C
My god, obtain strawberry.
2. Strawberry tissue culture method according to claim 1, it is characterised in that first culture medium is cultivated including MS
The parts by weight of parts by weight of base 50~60, mass concentration for 1.0mg/L the parts by weight of parts by weight of 6- benzyls aminoadenine 1~10, quality
The parts by weight of the parts by weight of parts by weight of methyl α-naphthyl acetate 0.1 that concentration is 0.1mg/L~1, the parts by weight of white sugar 1~10 and mass concentration are
The parts by weight of 3.8g/L parts by weight of agar 1~10.
3. Strawberry tissue culture method according to claim 1, it is characterised in that second culture medium is cultivated including MS
The parts by weight of parts by weight of base 50~60, mass concentration for 0.01mg/L the parts by weight of parts by weight of methyl α-naphthyl acetate 0.1~1, the parts by weight of white sugar 1
~10 parts by weight and mass concentration for 3.8g/L the parts by weight of parts by weight of agar 1~10.
4. Strawberry tissue culture method according to claim 1, it is characterised in that the 3rd culture medium includes weight ratio
For 1:1 broad-leaved fertile soil and the dregs of a decoction, the dregs of a decoction include：30 parts~50 parts of ginkgo leaf, 1 part~10 parts of radix scutellariae, 1 part of glossy privet seed
0.1 part~1 part of~10 parts, 0.1 part~1 part of cordate houttuynia, 0.1 part~1 part of polygonum capitatum and sealwort.
5. Strawberry tissue culture method according to claim 1, it is characterised in that in the step S104, described
Potassium dihydrogen phosphate and quality of the foliage-spray mass concentration for 0.01%~1% when being cultivated the 14th day~17 days in the 3rd culture medium
Concentration is 0.01%~1% urea, hereafter, and foliage-spray mass concentration is 0.01%~1% biphosphate twice a week
Potassium and the urea that mass concentration is 0.01%~1%.
6. Strawberry tissue culture method according to claim 1, it is characterised in that in the step S104, described
When being cultivated in the 3rd culture medium, the plant taken root is watered every 10 days~15 days, and adds in water carbendazim；
Wherein, the weight ratio of the water and the carbendazim is 1000：1.
7. Strawberry tissue culture method according to claim 1, it is characterised in that in the step S104, described
The nutrient solution for the MS culture mediums that mass concentration is 50% is sprayed when being cultivated the 7th day~10 days in the 3rd culture medium.
8. Strawberry tissue culture method according to claim 1, it is characterised in that in the step S101, the grass
Certain kind of berries explant is the stolon of strawberry.
9. the strawberry that the Strawberry tissue culture method described in claim any one of 1-8 is obtained.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102726179A (en) * | 2011-04-13 | 2012-10-17 | 林光德 | Cultivation method of red-color strawberries |
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