CN107164433A - A kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose - Google Patents
A kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose Download PDFInfo
- Publication number
- CN107164433A CN107164433A CN201710583320.8A CN201710583320A CN107164433A CN 107164433 A CN107164433 A CN 107164433A CN 201710583320 A CN201710583320 A CN 201710583320A CN 107164433 A CN107164433 A CN 107164433A
- Authority
- CN
- China
- Prior art keywords
- lignocellulosic
- potassium permanganate
- residue
- alkalinity potassium
- pretreatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose, this method is comprised the following steps that：Lignocellulosic material is crushed, dried, controls ultrasonic power, alkalinity potassium permanganate concentration, solid-to-liquid ratio, pretreatment time and temperature in suitable scope respectively, filtering is washed to neutrality, and pretreated lignocellulosic residue is obtained after drying；Digested into residue obtained above in citrate buffer solution system using commercial cellulose enzyme and zytase, obtain fermentable reduction sugar juice.Preprocess method in the present invention improves the retention rate of the clearance of lignin, cellulose and hemi-cellulose components using the collaboration energy of ultrasonic wave, and effect substantially, reduces the consumption of alkalinity potassium permanganate；The lignocellulosic residue enzymolysis efficiency and Reducing sugar that the present invention is pre-processed are greatly enhanced.Meanwhile, the inventive method has environment-friendly, simple to operate, low cost and other advantages.
Description
Technical field
The invention belongs to lignocellulose biomass application technology as the second resource field, and in particular to one kind is auxiliary using ultrasonic wave
The method for helping alkalinity potassium permanganate preprocessing lignocellulose.
Background technology
Lignocellulosic is the renewable resource of reserves maximum in nature, can pass through chemistry and bioconversion generates energy
Source substitute materials or chemical products, under fossil energy material increasingly depleted and the dual-pressure of environmental protection, lignocellulosic
Effective trans-utilization turn into and realize the important subject of sustainable development, this problem has been included in country, long-term section
Learn development plan.At present, the microorganism conversion of lignocellulosic is most commonly used that acidic hydrolysis.The main difficulty of acidic hydrolysis
Point is producing for preconditioning technique and low-cost high-efficiency enzyme.Lignocellulosic is by groups such as cellulose, hemicellulose and lignin
Into complicated, cellulose and hemicellulose are wrapped up layer by layer by lignin, and lignin plays screen during hydrocellulose
Barrier is acted on.Therefore, the effect of pretreatment is to reduce the degree of polymerization and crystallinity of cellulose, it is impossible to be degraded to the wood of fermentable sugars
The separation of quality component is sloughed, and increases the reaction surface area of cellulose.
In recent years, it is more to the research report in terms of the pretreatment of lignocellulosic material, including using traditional acid, alkali
The processing method such as pretreatment and gas explosion method, the quick-fried method of ammonia, ionic liquid and bioanalysis in recent years newly developed.Although to a certain extent
Improving the enzymatic hydrolyzation of lignocellulosic, but still there is complex procedures, energy consumption and equipment investment height causes pretreatment cost high
And pretreatment waste liquid bring environmental pollution a series of problems, such as.Alkaline reagent and the preprocess method quilt of strong oxidizer combination
Report can effectively remove the lignin in biomass, while the hydrogen bond and the crystalline substance of cellulose that destroy between hemicellulose, cellulose
Body structure, so that the structure of stubbornly resisting destroyed in biomass, but research papers are less.
Potassium permanganate is a kind of strong oxidizer, is widely used in Water purification, both can by the oxidation operation in water,
Organic substance in water can be adsorbed etc., play deodorization, demanganization and except iron etc. is acted on.Our early-stage Study is used first
Alkalinity potassium permanganate preprocessing lignocellulose, as a result shows, this method can effectively destroy three components of lignocellulosic it
Between the connection of chemical combination key, remove most of lignin in corncob, improve the enzymolysis yield of follow-up lignocellulosic.Using alkalescence
Permanganate pretreatment lignocellulosic, compared with the oxidants such as ozone, chlorine dioxide and chlorine, the security of potassium permanganate is more
Height, cost higher compared with alkaline hydrogen peroxide stability is lower, therefore, with feasible industrial applications prospect.
The content of the invention
The present invention establishes a kind of alkaline permanganate pretreatment lignocellulosic of collaboration energy auxiliary of utilization ultrasonic wave
Process, it is intended to the retention rate of the clearance of lignin, cellulose and hemi-cellulose components is improved, while it is high to reduce alkalescence
The consumption of potassium manganate, the final enzymolysis efficiency for improving lignocellulosic, reduction pretreatment cost.Specifically include following steps：
(1) it is lignocellulosic material is (any in maize straw, corncob, wheat stalk, rice straw and bagasse
A kind of or its combination) crush, 40~80 mesh sieves are crossed after 90 DEG C of drying.
(2) raw material of a certain amount of size-reduced drying is taken, adding alkaline permanganate solution makes solid-to-liquid ratio control 1: 10~1
: 30, it is preferred with 1: 10；Make the concentration of alkalinity potassium permanganate control to be 0.5~4% (w/v), be preferred with 2%；Pretreatment temperature control
System is preferred at 30~70 DEG C with 60 DEG C；Pretreatment time is controlled in 0.5~8h, is preferred with 4h.
(3) after lignocellulosic residue filtering after pretreatment, with distillation water washing until filtrate pH reaches neutrality, 90
DEG C dry to constant weight.
(4) residue obtained through above-mentioned pretreatment is added during 0.05mol/L pH are 4.5~5.0 citrate buffer solutions,
It is 2~10% (w/v) to control its concentration, is preferred with 8%.
(5) the plain enzyme of commercial fibers and zytase from trichoderma are added into above-mentioned system, enzyme is controlled respectively
Useful load is 20~50FPU/g substrates (being preferred with 30FPU/g substrates) and 5000~12000U/g substrates (with 8000U/g substrates
It is preferred), 45~55 DEG C, 48~96h of reaction is digested under conditions of 100~200rpm of shaking speed, with 50 DEG C, shaking speed
150rpm, enzymolysis 72h is preferred.
(6) reduced sugar and glucose, xylose concentration in hydrolyzate are determined with DNS methods and HPLC methods respectively.
The present invention has the advantage that：The present invention is first using the method for ultrasonic assistant alkalinity potassium permanganate to wood fibre
Plain raw material is pre-processed, under the synergy of ultrasonic energy, can strengthen alkaline permanganate solution to wood fibre
The infiltration of element and swelling action, raw material microstructure analysis shows, after being pre-processed through this method, and lignocellulosic material surface is fluffy
Porous, structure becomes loose, and mechanical strength weakens, the valence link that alkalinity potassium permanganate can be destroyed between hemicellulose and lignin
Connection, oxidation ehter bond and ester bond removes lignin in dissolving corncob, this structure be conducive to cellulase and zytase with
The contact of corncob, beneficial to the enzymolysis in later stage.Ultrasonic assistant can improve the clearance of lignin, reduce potassium permanganate alkalescence molten
The consumption of liquid, after being pre-processed through the inventive method, delignification rate is up to more than 50%, and cellulose and hemicellulose retention rate divide
Not up to 94% and more than 85%.The enzymatic hydrolyzation of lignocellulosic is greatly improved.The inventive method mild condition, simply
It is easy to operate, safety non-pollution, with feasible industrial applications prospect.
Embodiment
Below by embodiment, the present invention will be further described, and following embodiments are illustrative, be not it is limited,
Protection scope of the present invention can not be limited with following embodiments.
Embodiment 1
A kind of method that use ultrasonic assistant alkalinity potassium permanganate pre-processes maize straw, comprises the following steps：
(1) by corn straw smashing, 60 mesh sieves are crossed after 90 DEG C of drying to constant weight.
(2) corn stalk raw material of the size-reduced drying of 8.0g is taken, 100mL concentration is added molten for 2% (w/v) potassium permanganate
Liquid, sodium hydroxide adjusts pH to be 11, and the triangular flask that will be equipped with the mixed liquor is placed in ultrasonic response device, and setting power is
250W, frequency 40kHz, 4h is handled at 60 DEG C.
(3) pretreated maize straw is filtered, with distillation water washing until filtrate pH reaches neutrality, 90 DEG C of drying are extremely
Constant weight.
(4) (or pretreated maize straw is not former for the maize straw residue for taking after the above-mentioned pretreatments of 2.0g through drying
Material), 25mL 0.05mol/L, pH is added in 4.8 citrate buffer solution, the commodity of Trichoderma source are added into the mixed liquor
Cellulose enzyme and zytase, the useful load of its cellulase is 20FPU/g substrates, and the useful load of zytase is
12000U/g substrates, it is 40mL to control hydrolyzation system cumulative volume.
(5) above-mentioned system is digested under conditions of 50 DEG C, shaking speed 150rpm, is determined respectively also after reaction 72h
Raw sugar, glucose and xylose concentration.
By determining and calculating, cellulose retention rate is 94.28% after pretreatment, and hemicellulose retention rate is 87.56%,
Lignin removing rate is 56.79%.The enzymolysis more untreated maize straw of Reducing sugar improves 106.33%.
Embodiment 2
A kind of method that use ultrasonic assistant alkalinity potassium permanganate pre-processes corncob, comprises the following steps：
(1) corncob is crushed, crosses 80 mesh sieves after 90 DEG C of drying to constant weight.
(2) the corn core raw material of the size-reduced drying of 5.0g is taken, 100mL concentration is added molten for 1.5% (w/v) potassium permanganate
Liquid, sodium hydroxide adjusts pH to be 11.5, and the triangular flask that will be equipped with the mixed liquor is placed in ultrasonic response device, and setting power is
300W, frequency 40kHz, 4h is handled at 65 DEG C.
(3) pretreated corncob is filtered, with distillation water washing until filtrate pH reaches neutrality, 90 DEG C of drying to perseverance
Weight.
(4) the corncob residue (or not pretreated corn core raw material) after the above-mentioned pretreatments of 2.0g through drying is taken, plus
Enter in the citrate buffer solution that 25mL 0.05mol/L, pH are 4.8, the commercial fibers of Trichoderma source are added into the mixed liquor
Plain enzyme and zytase, the useful load of its cellulase is 30FPU/g substrates, and the useful load of zytase is 16000U/g bottoms
Thing, it is 40mL to control hydrolyzation system cumulative volume.
(5) above-mentioned system is digested under conditions of 50 DEG C, shaking speed 180rpm, is determined respectively also after reaction 72h
Raw sugar, glucose and xylose concentration.
By determining and calculating, cellulose retention rate is 94.26% after pretreatment, and hemicellulose retention rate is 85.83%,
Lignin removing rate is 58.17%.The enzymolysis more untreated corncob of Reducing sugar improves 92.21%.
Claims (6)
1. a kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose, it is characterised in that including following step
Suddenly：
(1) lignocellulosic material is crushed, dried, respectively control ultrasonic power, alkalinity potassium permanganate concentration, solid-to-liquid ratio,
Pretreatment time and temperature are filtered in suitable scope, and washing obtains pretreated lignocellulosic residue after drying；
(2) carried out into residue obtained above in citrate buffer solution system using commercial cellulose enzyme and zytase
Enzymolysis, determines concentration of reduced sugar in hydrolyzate.
2. according to the method described in claim 1, it is characterised in that described lignocellulosic is maize straw, corncob, small
Any one of Wheat Straw, rice straw and bagasse or its combination.
3. according to the method described in claim 1, it is characterised in that after lignocellulosic material is crushed in step (1), cross 40~
80 mesh sieves, 90 DEG C of dryings to constant weight.
4. according to the method described in claim 1, it is characterised in that ultrasonic power used is 150-800W in step (1), is surpassed
Frequency of sound wave is 20-50kHz；The concentration of alkalinity potassium permanganate is 0.5~4% (w/v)；Lignocellulosic material and SODIUM PERCARBONATE
The solid-to-liquid ratio of solution mixing is controlled 1: 10~1: 30；Pretreatment temperature is controlled at 30~70 DEG C；Pretreatment time is controlled 0.5
~8h；Lignocellulosic residue after pretreatment is filtered, with distillation water washing until filtrate pH reaches neutrality, 90 DEG C of dryings
To constant weight.
5. according to the method described in claim 1, it is characterised in that citrate buffer solution described in step (2) is 0.05mol/
L, pH are 4.5~5.0；Cellulase and zytase are from trichoderma commercialization enzyme preparation.
6. according to the method described in claim 1, it is characterised in that in step (2) concentration of lignocellulosic residue be 2~
10% (w/v)；The useful load of lignocellulosic residue enzymatic hydrolysis system cellulase is 10~30FPU/g substrates, zytase
Useful load be 8000~20000U/g substrates；Enzymolysis is anti-under conditions of 45~55 DEG C of temperature, 100~200rpm of shaking speed
Answer 48~96h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710583320.8A CN107164433A (en) | 2017-07-12 | 2017-07-12 | A kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710583320.8A CN107164433A (en) | 2017-07-12 | 2017-07-12 | A kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107164433A true CN107164433A (en) | 2017-09-15 |
Family
ID=59817896
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710583320.8A Pending CN107164433A (en) | 2017-07-12 | 2017-07-12 | A kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107164433A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628844A (en) * | 2019-09-23 | 2019-12-31 | 福建生物工程职业技术学院 | Graded separation method for cord grass |
| CN110791540A (en) * | 2019-11-08 | 2020-02-14 | 孟保奎 | Method for quickly pretreating biomass by microwave alkaline baking |
| CN112111541A (en) * | 2019-06-20 | 2020-12-22 | 南京理工大学 | Alkali method for pretreating densified lignocellulose and biotransformation method |
| CN112844324A (en) * | 2019-11-26 | 2021-05-28 | 南京理工大学 | Lignin/manganese oxide composite adsorption material and preparation method and application thereof |
| CN114086411A (en) * | 2021-11-25 | 2022-02-25 | 集美大学 | Honey pomelo young fruit cellulose and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821398A (en) * | 2007-08-27 | 2010-09-01 | 埃欧金能源公司 | Method for the production of fermentation product from pretreated lignocellulosic feedstock |
| CN102261007A (en) * | 2010-05-26 | 2011-11-30 | 漳州伯能生物能源有限公司 | Method for grading and separating all components of forestry cellulose biomass and preparing fuel alcohol and xylo-oligosaccharide by utilizing separated components |
| CN102839198A (en) * | 2012-09-13 | 2012-12-26 | 东南大学 | Method for enhancing alkaline hydrogen peroxide to pretreat lignocellulose by ultrasonic wave |
-
2017
- 2017-07-12 CN CN201710583320.8A patent/CN107164433A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821398A (en) * | 2007-08-27 | 2010-09-01 | 埃欧金能源公司 | Method for the production of fermentation product from pretreated lignocellulosic feedstock |
| CN102261007A (en) * | 2010-05-26 | 2011-11-30 | 漳州伯能生物能源有限公司 | Method for grading and separating all components of forestry cellulose biomass and preparing fuel alcohol and xylo-oligosaccharide by utilizing separated components |
| CN102839198A (en) * | 2012-09-13 | 2012-12-26 | 东南大学 | Method for enhancing alkaline hydrogen peroxide to pretreat lignocellulose by ultrasonic wave |
Non-Patent Citations (2)
| Title |
|---|
| 刘梅等: ""酸性高锰酸钾溶液处理对秸秆消化率的影响"", 《饲料工业》 * |
| 刘研萍等: ""秸秆厌氧消化中碱与高锰酸钾预处理对比研究"", 《现代化工》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112111541A (en) * | 2019-06-20 | 2020-12-22 | 南京理工大学 | Alkali method for pretreating densified lignocellulose and biotransformation method |
| CN110628844A (en) * | 2019-09-23 | 2019-12-31 | 福建生物工程职业技术学院 | Graded separation method for cord grass |
| CN110791540A (en) * | 2019-11-08 | 2020-02-14 | 孟保奎 | Method for quickly pretreating biomass by microwave alkaline baking |
| CN112844324A (en) * | 2019-11-26 | 2021-05-28 | 南京理工大学 | Lignin/manganese oxide composite adsorption material and preparation method and application thereof |
| CN114086411A (en) * | 2021-11-25 | 2022-02-25 | 集美大学 | Honey pomelo young fruit cellulose and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107164433A (en) | A kind of method of ultrasonic assistant alkalinity potassium permanganate preprocessing lignocellulose | |
| Jeihanipour et al. | Ethanol production from cotton-based waste textiles | |
| CN107164424A (en) | It is a kind of to aoxidize the lignocellulose pretreatment method that delignification improves enzyme hydrolysis rate | |
| US8815561B2 (en) | Metal compounds to eliminate nonproductive enzyme adsorption and enhance enzymatic saccharification of lignocellulose | |
| US8609379B2 (en) | Process for the production of alcohols from biomass | |
| Geng et al. | Ethanol production from horticultural waste treated by a modified organosolv method | |
| CN105385724A (en) | Method for improving conversion efficiency of lignocellulose through combined treatment and method for efficiently preparing ethyl alcohol | |
| CN104830928B (en) | A kind of lignocellulose pretreatment method of microwave radiation technology alkaline sodium sulfite | |
| WO2011061400A1 (en) | Method of processing a carbohydrate raw-material | |
| CN101255479A (en) | Pre-treatment method for highly-effective saccharification of lignocellulose | |
| CN101381754A (en) | Method for producing fermentable sugars by hydrolysis of cellulosic component sulphonation separation couple enzyme | |
| CN108048509A (en) | A kind of preprocess method of non-three plain ingredient of efficient removal lignocellulosic | |
| JP2008043328A (en) | Method for saccharifying wood-based biomass | |
| Wagner et al. | Valorization of brewer's spent grain by different strategies of structural destabilization and enzymatic saccharification | |
| JP4947223B1 (en) | Enzymatic saccharification method for lignocellulose-containing biomass | |
| JP2009213389A (en) | System for producing bioethanol using lignocellulose as raw material | |
| CN106191158B (en) | Clean and safe wood fiber raw material pretreatment method | |
| CN103146781B (en) | A kind of Cotton stalk hydrolysate and preparation method thereof | |
| CN107904271A (en) | A kind of method of microwave reinforced soda lime preprocessing lignocellulose | |
| CN106906265A (en) | A kind of straw pretreatment method produced without waste liquid | |
| Ji et al. | Electrogenerated alkaline hydrogen peroxide pretreatment of waste wheat straw to enhance enzymatic hydrolysis | |
| CN103031339B (en) | Method for producing ethanol by wood fiber raw material | |
| CN104561130A (en) | Microwave-assisted pretreatment method for maize straws | |
| WO2008074197A1 (en) | A method for converting herbaceous plant fibre to fuel alcohol | |
| Liao et al. | Effect of preferential delignification on xylooligosaccharides production from poplar by acetic acid/sodium acetate hydrolysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170915 |