CN107164304A - A kind of method of convenient cell recovery - Google Patents
A kind of method of convenient cell recovery Download PDFInfo
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- CN107164304A CN107164304A CN201710527043.9A CN201710527043A CN107164304A CN 107164304 A CN107164304 A CN 107164304A CN 201710527043 A CN201710527043 A CN 201710527043A CN 107164304 A CN107164304 A CN 107164304A
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Abstract
The invention provides a kind of method of convenient cell recovery, comprise the following steps：The cell cryopreservation tube frozen is taken out from liquid nitrogen or 80 DEG C of refrigerators, rock to the cell liquid containing cells frozen storing liquid and melt completely in 37 DEG C in 1min after taking-up, cell liquid is even added in the Tissue Culture Dish for filling 7 9ml fresh cultures, rock clockwise under Tissue Culture Dish 35, in 37 DEG C, 5%CO2Cultivated in incubator.The method of convenient cell recovery of the present invention, its method rationally, using convenient, can be rapidly used for biological and healthy correlative study.
Description
Technical field
The invention belongs to biological technology application, in particular it relates to a kind of method of convenient cell recovery.
Background technology
Cell culture is a biological important technology with healthy Related Research Domain.With the rapid hair of life science
Exhibition, current cell culture turns into the important base of the disciplinary studies such as cell biology, molecular biology, science of heredity and immunology
Plinth.In order to further investigated cell vegetative activity rule, have the pathology of related disorders and the pharmacology of medicine（Toxicity）Mechanism, exploitation tool
There are different targetedly cell culture processes significant.
Cell recovery is the one kind for cultivating cell, is wide variety of key technology in life and regenerative medicine field,
Generally require to recover to the cell frozen in research.Especially as the rise of cell therapy personalization technology, amplification in vitro
The cell of special efficacy is into preclinical study and the emphasis development project of clinical treatment.With amplification in vitro special efficacy
Cell is included by multi-functional candidate stem cell, T cell, NK cells, DC cells and CIK cell etc., by this kind of cells in vitro
Fed back to again in lactation organism after amplification, be conducive to improving the hematopoietic device function of body：Such as lift red blood cell, be immunized carefully
The maintenance of quantity and function, anti-tumor capacity and the immune defense ability of born of the same parents etc..
The content of the invention
Goal of the invention：It is thin for a variety of people sources and inhuman source the invention provides a kind of method of convenient cell recovery
The recovery of born of the same parents.
Technical scheme：The invention provides a kind of method of convenient cell recovery, comprise the following steps：By the cell frozen
Cryopreservation tube takes out from liquid nitrogen or -80 DEG C of refrigerators, is rocked after taking-up in 1min in 37 DEG C to containing the thin of cells frozen storing liquid
Cytosol melts completely, cell liquid is even added in the Tissue Culture Dish for filling 7-9ml fresh cultures, rocks thin clockwise
Under born of the same parents' culture dish 3-5, in 37 DEG C, 5%CO2Cultivated in incubator.The method of convenient cell recovery of the present invention, its method
Rationally, using convenient, biological and healthy correlative study can be rapidly used for.
Further, the method for above-mentioned convenient cell recovery, the basal medium of the culture medium including 70-90% with
And 10-30% hyclone.Nutrient media components is rationally, nutritious, and more nutrients can be provided for cell culture（Bag
Include growth factor etc.）, high cell growth speed, cell state is good.
Further, the method for above-mentioned convenient cell recovery, the cells frozen storing liquid is by weight part, including following
Component：5-15 parts of dimethyl sulfoxide (DMSO), 2-5 parts of HES, 5-10 parts of chitosan, 2-5 parts of glutathione, vitamin C 1-4
Part, 0.3-4 parts of dextran, 0.5-2 parts of propane diols, N- (benzyl) -2- (4- pyrimdinyl-aminos) -4- thiazole carboxamides 0.1-1
80-90 parts of part and basal medium.Frozen stock solution component is rationally, nutritious, and wherein dextran is permeability protection liquid, and N-
(benzyl) -2- (4- pyrimdinyl-aminos) -4- thiazole carboxamides then suppress cellular activity and gene expression, ascorbic anti-oxidant
Property it is good, can also substantially reduce the damage to cell while the nutrition of rest cell is ensured, improve the survival of freeze-stored cell
Rate, and also keep in terms of propagation good state.
Further, the method for above-mentioned convenient cell recovery, the basal medium is by weight part, including following
Component：80-90 parts of glucose, 10-20 parts of sodium acid carbonate, 8-18 parts of Sodium Pyruvate, 3-12 parts of sericin, potassium chloride 30-
50 parts, 5-12 parts of anhydrous magnesium sulfate, 60-80 parts of sodium chloride, 8-16 parts of AMSP, 0.2-0.6 parts of folic acid, flesh
0.5-1.5 parts of alcohol, 0.2-0.8 parts of niacinamide, 20-40 parts of anhydrous calcium chloride, 0.1-0.5 parts of ferric nitrate, 5-10 parts of succinic acid,
9-18 parts of sodium succinate, 0.2-0.8 parts of D-VB5 calcium, 0.5-1 parts of choline tartrate, 0.1-0.3 parts of riboflavin, thiamine hydrochloride
0.1-0.5 parts, 0.1-0.6 parts of pyridoxine hydrochloride, 0.1-0.5 parts of NIPA, 0.3-3 parts of leukotrienes, monoethanolamine
0.5-0.8 parts, 0.2-0.9 parts of aromatic esters, phenol red sodium 0.8-1.5 parts and 100 parts of deionized water.
Further, the method for above-mentioned convenient cell recovery, the basal medium also includes 3-8 parts of amino acid, institute
State amino acid by weight part, it is composed of the following components：5-10 parts of L- R-genes, 3-8 parts of L- hydrochloric acid cystines, L-
3-6 parts of serine, 1-5 parts of glycine, 4-6 parts of L- histidine monohydrochlorides, 8-20 parts of ILE, 7-18 parts of L-Leu, L-
10-20 parts of lysine hydrochloride, 1-6 parts of METHIONINE, 2-8 parts of L-phenylalanine, 5-15 parts of L-threonine, L-Trp 1-3
8-12 parts of part, 5-9 parts of TYR and Valine.
Further, the method for above-mentioned convenient cell recovery, the basal medium also includes 2-5 parts of confactors,
The confactor is by weight part, composed of the following components：It is 2-10 parts of recombination human basic fibroblast growth factor, white
1-4 parts of 3-8 parts of interleukin 2,3-6 parts of IL-4,1-5 parts of interleukin-11 4,3-6 parts of IFN-γ and neuroleukin.
Further, the method for above-mentioned convenient cell recovery, the basal medium by weight part, in addition to
0.006 part of penicillin and 0.01 part of streptomysin.The cell of recovery can be effectively prevented to be contaminated.
Further, the method for above-mentioned convenient cell recovery, the penicillin is selected from 10000U/ml, the streptomysin
Selected from 10000 μ g/ml.Penicillin and streptomysin activity are good, and effect is good.
Further, the method for above-mentioned convenient cell recovery, the Tissue Culture Dish is 10cm Tissue Culture Dish.
Beneficial effect：The method of convenient cell recovery of the present invention, its method rationally, can be notable using convenient
The damage frozen to cell is reduced, the cell survival rate high state after recovery is good, cell boundaries are clear, high cell growth speed,
Biological and healthy correlative study can be rapidly used for.
Embodiment
Below will be by several specific embodiments, the present invention is furture elucidated, these embodiments simply to illustrate that problem,
It is not a kind of limitation.
Embodiment 1
A kind of method of convenient cell recovery, comprises the following steps：By the cell cryopreservation tube frozen from liquid nitrogen or -80 DEG C of refrigerators
It is middle to take out, rock to the cell liquid containing cells frozen storing liquid and melt completely in 37 DEG C in 1min after taking-up, cell liquid is uniform
It is added in the 10cm Tissue Culture Dish for filling 7ml fresh cultures, rocks clockwise under Tissue Culture Dish 3, in 37 DEG C, 5%
CO2Cultivated in incubator.
Wherein, the culture medium includes 70% basal medium and 30% hyclone.In addition, the cells frozen storing liquid
By weight part, including following components：5 parts of dimethyl sulfoxide (DMSO), 2 parts of HES, 5 parts of chitosan, 2 parts of glutathione,
1 part of vitamin C, 0.3 part of dextran, 0.5 part of propane diols, N- (benzyl) -2- (4- pyrimdinyl-aminos) -4- thiazole carboxamides
0.1 part and 80 parts of basal medium.
Further, the method for above-mentioned convenient cell recovery, the basal medium is by weight part, including following
Component：80 parts of glucose, 10 parts of sodium acid carbonate, 8 parts of Sodium Pyruvate, 3 parts of sericin, 30 parts of potassium chloride, anhydrous magnesium sulfate
5 parts, 60 parts of sodium chloride, 8 parts of AMSP, 0.2 part of folic acid, 0.5 part of inositol, 0.2 part of niacinamide, anhydrous calcium chloride
20 parts, 0.1 part of ferric nitrate, 5 parts of succinic acid, 9 parts of sodium succinate, 0.2 part of D-VB5 calcium, 0.5 part of choline tartrate, riboflavin
0.1 part, 0.1 part of thiamine hydrochloride, 0.1 part of pyridoxine hydrochloride, 0.1 part of NIPA, 0.3 part of leukotrienes, monoethanolamine
100 parts of 0.5 part, 0.2 part of aromatic esters, 0.8 part of phenol red sodium and deionized water.
In addition, the basal medium also includes 3 parts of amino acid, the amino acid by weight part, by following components group
Into：5 parts of L- R-genes, 3 parts of L- hydrochloric acid cystine, 3 parts of Serine, 1 part of glycine, 4 parts of L- histidine monohydrochlorides,
8 parts of ILE, 7 parts of L-Leu, 10 parts of LYS, 1 part of METHIONINE, 2 parts of L-phenylalanine, L- Soviet Unions ammonia
8 parts of 5 parts of acid, 1 part of L-Trp, 5 parts of TYR and Valine.
Again, the basal medium also includes 2 parts of confactors, the confactor by weight part, by with the following group
It is grouped into：2 parts of recombination human basic fibroblast growth factor, 3 parts of interleukin-22,3 parts of IL-4,41 parts of interleukin-11,
1 part of 3 parts of IFN-γ and neuroleukin.
In addition, the basal medium is by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.
Also, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 2
A kind of method of convenient cell recovery, comprises the following steps：By the cell cryopreservation tube frozen from liquid nitrogen or -80 DEG C of refrigerators
It is middle to take out, rock to the cell liquid containing cells frozen storing liquid and melt completely in 37 DEG C in 1min after taking-up, cell liquid is uniform
It is added in the 10cm Tissue Culture Dish for filling 9ml fresh cultures, rocks clockwise under Tissue Culture Dish 5, in 37 DEG C, 5%
CO2Cultivated in incubator.
Wherein, the culture medium includes 90% basal medium and 10% hyclone.In addition, the cells frozen storing liquid
By weight part, including following components：15 parts of dimethyl sulfoxide (DMSO), 5 parts of HES, 10 parts of chitosan, glutathione 5
Part, 4 parts of vitamin C, 4 parts of dextran, 2 parts of propane diols, N- (benzyl) -2- (4- pyrimdinyl-aminos) -4- thiazole carboxamides 1
90 parts of part and basal medium.
Further, the method for above-mentioned convenient cell recovery, 90 parts of glucose, 20 parts of sodium acid carbonate, Sodium Pyruvate 18
Part, 12 parts of sericin, 50 parts of potassium chloride, 12 parts of anhydrous magnesium sulfate, 80 parts of sodium chloride, 16 parts of AMSP, folic acid
0.6 part, 1.5 parts of inositol, 0.8 part of niacinamide, 40 parts of anhydrous calcium chloride, 0.5 part of ferric nitrate, 10 parts of succinic acid, sodium succinate 18
Part, 0.8 part of D-VB5 calcium, 1 part of choline tartrate, 0.3 part of riboflavin, 0.5 part of thiamine hydrochloride, 0.6 part of pyridoxine hydrochloride, N-
0.5 part of N-isopropylacrylamide, 3 parts of leukotrienes, 0.8 part of monoethanolamine, 0.9 part of aromatic esters, 1.5 parts of phenol red sodium and deionized water
100 parts.
In addition, the basal medium also includes 8 parts of amino acid, the amino acid by weight part, by following components group
Into：10 parts of L- R-genes, 8 parts of L- hydrochloric acid cystine, 6 parts of Serine, 5 parts of glycine, 6 parts of L- histidine monohydrochlorides,
20 parts of ILE, 18 parts of L-Leu, 20 parts of LYS, 6 parts of METHIONINE, 8 parts of L-phenylalanine, L- Soviet Unions
12 parts of 15 parts of propylhomoserin, 3 parts of L-Trp, 9 parts of TYR and Valine.
Again, the basal medium also includes 5 parts of confactors, the confactor by weight part, by with the following group
It is grouped into：10 parts of recombination human basic fibroblast growth factor, 8 parts of interleukin-22,6 parts of IL-4,45 parts of interleukin-11,
4 parts of 6 parts of IFN-γ and neuroleukin.
In addition, the basal medium is by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.
Also, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 3
A kind of method of convenient cell recovery, comprises the following steps：By the cell cryopreservation tube frozen from liquid nitrogen or -80 DEG C of refrigerators
It is middle to take out, rock to the cell liquid containing cells frozen storing liquid and melt completely in 37 DEG C in 1min after taking-up, cell liquid is uniform
It is added in the 10cm Tissue Culture Dish for filling 8ml fresh cultures, rocks clockwise under Tissue Culture Dish 4, in 37 DEG C, 5%
CO2Cultivated in incubator.
Wherein, the culture medium includes 80% basal medium and 20% hyclone.In addition, the cells frozen storing liquid
By weight part, including following components：10 parts of dimethyl sulfoxide (DMSO), 3 parts of HES, 6 parts of chitosan, 3 parts of glutathione,
Catergen part, 3 parts of dextran, 1 part of propane diols, 0.8 part of N- (benzyl) -2- (4- pyrimdinyl-aminos) -4- thiazole carboxamides
With 85 parts of basal medium.
Further, the method for above-mentioned convenient cell recovery, the basal medium is by weight part, including following
Component：86 parts of glucose, 18 parts of sodium acid carbonate, 12 parts of Sodium Pyruvate, 6 parts of sericin, 40 parts of potassium chloride, anhydrous magnesium sulfate 8
Part, 75 parts of sodium chloride, 12 parts of AMSP, 0.4 part of folic acid, 1 part of inositol, 0.5 part of niacinamide, anhydrous calcium chloride 30
Part, 0.3 part of ferric nitrate, 7 parts of succinic acid, 14 parts of sodium succinate, 0.5 part of D-VB5 calcium, 0.7 part of choline tartrate, riboflavin
0.2 part, 0.3 part of thiamine hydrochloride, 0.4 part of pyridoxine hydrochloride, 0.3 part of NIPA, 1.6 parts of leukotrienes, monoethanolamine
100 parts of 0.7 part, 0.5 part of aromatic esters, 1.2 parts of phenol red sodium and deionized water.
In addition, the basal medium also includes 6 parts of amino acid, the amino acid by weight part, by following components group
Into：8 parts of L- R-genes, 6 parts of L- hydrochloric acid cystine, 5 parts of Serine, 3 parts of glycine, 5 parts of L- histidine monohydrochlorides,
12 parts of ILE, 10 parts of L-Leu, 15 parts of LYS, 3 parts of METHIONINE, 4 parts of L-phenylalanine, L- Soviet Unions
9 parts of 9 parts of propylhomoserin, 2 parts of L-Trp, 6 parts of TYR and Valine.
Again, the basal medium also includes 3 parts of confactors, the confactor by weight part, by with the following group
It is grouped into：6 parts of recombination human basic fibroblast growth factor, 5 parts of interleukin-22,4 parts of IL-4,45 parts of interleukin-11,
3 parts of 5 parts of IFN-γ and neuroleukin.
In addition, the basal medium is by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.
Also, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 4
A kind of method of convenient cell recovery, comprises the following steps：By the cell cryopreservation tube frozen from liquid nitrogen or -80 DEG C of refrigerators
It is middle to take out, rock to the cell liquid containing cells frozen storing liquid and melt completely in 37 DEG C in 1min after taking-up, cell liquid is uniform
It is added in the 10cm Tissue Culture Dish for filling 9ml fresh cultures, rocks clockwise under Tissue Culture Dish 5, in 37 DEG C, 5%
CO2Cultivated in incubator.
Wherein, the culture medium includes 70 basal medium and 30% hyclone.In addition, the cells frozen storing liquid
By weight part, including following components：15 parts of dimethyl sulfoxide (DMSO), 2 parts of HES, 6 parts of chitosan, 3 parts of glutathione,
1 part of vitamin C, 4 parts of dextran, 1 part of propane diols, 0.8 part of N- (benzyl) -2- (4- pyrimdinyl-aminos) -4- thiazole carboxamides
With 90 parts of basal medium.
Further, the method for above-mentioned convenient cell recovery, the basal medium is by weight part, including following
Component：82 parts of glucose, 25 parts of sodium acid carbonate, 12 parts of Sodium Pyruvate, 6 parts of sericin, 45 parts of potassium chloride, anhydrous magnesium sulfate
6 parts, 72 parts of sodium chloride, 14 parts of AMSP, 0.3 part of folic acid, 1 part of inositol, 0.3 part of niacinamide, anhydrous calcium chloride 35
Part, 0.3 part of ferric nitrate, 6 parts of succinic acid, 12 parts of sodium succinate, 0.6 part of D-VB5 calcium, 0.7 part of choline tartrate, riboflavin
0.2 part, 0.3 part of thiamine hydrochloride, 0.4 part of pyridoxine hydrochloride, 0.4 part of NIPA, 2 parts of leukotrienes, monoethanolamine
100 parts of 0.6 part, 0.3 part of aromatic esters, 1.3 parts of phenol red sodium and deionized water.
In addition, the basal medium also includes 7 parts of amino acid, the amino acid by weight part, by following components group
Into：5 parts of L- R-genes, 8 parts of L- hydrochloric acid cystine, 6 parts of Serine, 3 parts of glycine, 5 parts of L- histidine monohydrochlorides,
12 parts of ILE, 18 parts of L-Leu, 10 parts of LYS, 5 parts of METHIONINE, 4 parts of L-phenylalanine, L- Soviet Unions
8 parts of 10 parts of propylhomoserin, 3 parts of L-Trp, 5 parts of TYR and Valine.
Again, the basal medium also includes 3 parts of confactors, the confactor by weight part, by with the following group
It is grouped into：2 parts of recombination human basic fibroblast growth factor, 8 parts of interleukin-22,3 parts of IL-4,44 parts of interleukin-11,
2 parts of 5 parts of IFN-γ and neuroleukin.
In addition, the basal medium is by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.
Also, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Described above is only several embodiments of invention, it is noted that for those skilled in the art
For, on the premise of inventive principle is not departed from, some improvement can also be made, these improvement also should be regarded as the protection of the present invention
Scope.
Claims (9)
1. a kind of method of convenient cell recovery, it is characterised in that：Comprise the following steps：By the cell cryopreservation tube frozen from liquid nitrogen
Or taken out in -80 DEG C of refrigerators, rock to the cell liquid containing cells frozen storing liquid and melt completely in 37 DEG C in 1min after taking-up,
Cell liquid is even added in the Tissue Culture Dish for filling 7-9ml fresh cultures, Tissue Culture Dish 3-5 is rocked clockwise
Under, in 37 DEG C, 5%CO2Cultivated in incubator.
2. the method for convenient cell recovery according to claim 1, it is characterised in that：The culture medium includes 70-90%'s
The hyclone of basal medium and 10-30%.
3. the method for convenient cell recovery according to claim 1, it is characterised in that：The cells frozen storing liquid is with weight group
Divide meter, including following components：5-15 parts of dimethyl sulfoxide (DMSO), 2-5 parts of HES, 5-10 parts of chitosan, glutathione 2-5
Part, 1-4 parts of vitamin C, 0.3-4 parts of dextran, 0.5-2 parts of propane diols, N- (benzyl) -2- (4- pyrimdinyl-aminos) -4- thiophenes
80-90 parts of 0.1-1 parts of azoles formamide and basal medium.
4. the method for the convenient cell recovery according to Claims 2 or 3, it is characterised in that：The basal medium is with weight
Measure component meter, including following components：80-90 parts of glucose, 10-20 parts of sodium acid carbonate, 8-18 parts of Sodium Pyruvate, sericin
3-12 parts, 30-50 parts of potassium chloride, 5-12 parts of anhydrous magnesium sulfate, 60-80 parts of sodium chloride, 8-16 parts of AMSP,
0.2-0.6 parts of folic acid, 0.5-1.5 parts of inositol, 0.2-0.8 parts of niacinamide, 20-40 parts of anhydrous calcium chloride, ferric nitrate 0.1-0.5
Part, 5-10 parts of succinic acid, 9-18 parts of sodium succinate, 0.2-0.8 parts of D-VB5 calcium, 0.5-1 parts of choline tartrate, riboflavin
0.1-0.3 parts, it is 0.1-0.5 parts of thiamine hydrochloride, 0.1-0.6 parts of pyridoxine hydrochloride, 0.1-0.5 parts of NIPA, sub-
Numb 0.3-3 parts of acid, 0.5-0.8 parts of monoethanolamine, 0.2-0.9 parts of aromatic esters, phenol red sodium 0.8-1.5 parts and 100 parts of deionized water.
5. the method for convenient cell recovery according to claim 4, it is characterised in that：The basal medium also includes 3-
8 parts of amino acid, the amino acid is by weight part, composed of the following components：5-10 parts of L- R-genes, L- hydrochloric acid Guangs
3-8 parts of propylhomoserin, 3-6 parts of Serine, 1-5 parts of glycine, 4-6 parts of L- histidine monohydrochlorides, 8-20 parts of ILE, L- are bright
7-18 parts of propylhomoserin, 10-20 parts of LYS, 1-6 parts of METHIONINE, 2-8 parts of L-phenylalanine, L-threonine 5-15
Part, 1-3 parts of L-Trp, 5-9 parts of TYR and 8-12 parts of Valine.
6. the method for convenient cell recovery according to claim 5, it is characterised in that：The basal medium also includes 2-
5 parts of confactors, the confactor is by weight part, composed of the following components：Recombination human basic fibroblast growth
2-10 parts of the factor, 3-8 parts of interleukin-22,3-6 parts of IL-4,1-5 parts of interleukin-11 4,3-6 parts of IFN-γ and neural leucocyte
It is plain 1-4 parts.
7. the method for convenient cell recovery according to claim 6, it is characterised in that：The basal medium is with weight group
Divide meter, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.
8. the method for convenient cell recovery according to claim 7, it is characterised in that：The penicillin is selected from 10000U/
Ml, the streptomysin is selected from 10000 μ g/ml.
9. the method for convenient cell recovery according to claim 1, it is characterised in that：The Tissue Culture Dish is that 10cm is thin
Born of the same parents' culture dish.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1698690A1 (en) * | 2003-12-26 | 2006-09-06 | Makoto Asashima | Basal medium for es cell culturing |
| CN102305747A (en) * | 2011-05-27 | 2012-01-04 | 苏州大学 | Biomarker reagent used for detecting breast cancer state |
-
2017
- 2017-06-30 CN CN201710527043.9A patent/CN107164304A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1698690A1 (en) * | 2003-12-26 | 2006-09-06 | Makoto Asashima | Basal medium for es cell culturing |
| CN102305747A (en) * | 2011-05-27 | 2012-01-04 | 苏州大学 | Biomarker reagent used for detecting breast cancer state |
Non-Patent Citations (5)
| Title |
|---|
| PHYSICAL SCIENCES-ONCOLOGY CENTER NETWORK BIORESOURCE CORE FACIL: "Protocal for thawing,propagation and cryopreservation of NCl-PBCF-HTB26(MDA-MB-231)(ATCC HTB-26TM) breast adenocarcinoma", 《AMERCIAN TYPE CULTURE COLLECTION》 * |
| 储炬: "《现代生物工艺学》", 30 September 2007 * |
| 刘冬: "《食品生物技术》", 30 June 2008 * |
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