CN107158447B - Antibacterial zein dressing with controllable orientation and preparation method thereof - Google Patents
Antibacterial zein dressing with controllable orientation and preparation method thereof Download PDFInfo
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- CN107158447B CN107158447B CN201710546579.5A CN201710546579A CN107158447B CN 107158447 B CN107158447 B CN 107158447B CN 201710546579 A CN201710546579 A CN 201710546579A CN 107158447 B CN107158447 B CN 107158447B
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- 229920002494 Zein Polymers 0.000 title claims abstract description 116
- 239000005019 zein Substances 0.000 title claims abstract description 116
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 80
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
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- 238000000034 method Methods 0.000 claims abstract description 23
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- 239000000243 solution Substances 0.000 claims description 58
- 239000000284 extract Substances 0.000 claims description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 10
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 claims description 8
- 230000000845 anti-microbial effect Effects 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 6
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- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- IKIIZLYTISPENI-ZFORQUDYSA-N baicalin Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 IKIIZLYTISPENI-ZFORQUDYSA-N 0.000 claims description 2
- 229960003321 baicalin Drugs 0.000 claims description 2
- AQHDANHUMGXSJZ-UHFFFAOYSA-N baicalin Natural products OC1C(O)C(C(O)CO)OC1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC=CC=1)O2 AQHDANHUMGXSJZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims description 2
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- 239000010642 eucalyptus oil Substances 0.000 description 3
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- 206010059866 Drug resistance Diseases 0.000 description 2
- 239000000956 alloy Substances 0.000 description 2
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
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- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920005594 polymer fiber Polymers 0.000 description 1
- 108060006613 prolamin Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
- A61L2300/214—Amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/22—Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Artificial Filaments (AREA)
- Nonwoven Fabrics (AREA)
- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
Abstract
The invention belongs to the technical field of medical materials, and discloses an antibacterial zein dressing with controllable orientation and a preparation method thereof. The preparation method comprises the following steps: respectively preparing a fiber dressing layer by taking a zein solution containing an antibacterial component as a receiver by using a grid template with a geometric figure which is regularly arranged through an electrostatic spinning method, and receiving by a roller receiver with a surface with a stripe and bulge structure which is regularly arranged to obtain a fiber diversion layer; laminating and compounding the fiber dressing layer and the fiber guide layer to obtain a zein fiber membrane functional layer; and then compounding the zein fiber membrane functional layer with the spunlaced fabric supporting layer and the polypropylene film barrier layer to obtain the antibacterial zein dressing with controllable orientation. The fiber membrane functional layer of the dressing has a microporous structure and a diversion structure, has good permeability or air permeability, and can realize directional diversion and avoid rewet, thereby keeping the wound dry.
Description
Technical Field
The invention belongs to the technical field of medical materials, and particularly relates to an antibacterial zein dressing with controllable orientation and a preparation method thereof.
Background
Zein is a natural polymer, is the most main storage protein of corn, is easy to obtain, and is economical and environment-friendly. Zein has good biocompatibility, biodegradability and the like, and is an environment-friendly, green and safe biological material.
Zein molecules not only have a large number of hydrophobic amino acids, but also contain more sulfur-containing amino acids, and have strong hydrophobicity. In a polar environment, hydrophilic groups in zein molecules are exposed, and hydrophobic groups are embedded, so that a stable micelle structure can be formed. The prolamin can be dissolved in a certain solvent, so that a solution can be prepared, a film or fibrous material can be prepared, and insoluble crosslinked zein film or fiber can be obtained after further crosslinking by adopting aldehydes. Zein is easy to combine with other compounds to form a composite high polymer, and a carrier conveying system with different characteristics can be constructed according to different requirements. Therefore, zein can be a good raw material for preparing the controllably oriented liquid conducting nanofiber.
The plant extract antibacterial agent is generated by the evolution of plants in order to adapt to the environment, and certain plant extracts have certain control or inhibition or elimination effects on certain diseases or certain bacteria, viruses and the like. For example: the extract of the compound plant has good inhibiting effect on aeromonas hydrophila, and the extraction and separation methods are known, and the operation is simple and easy. Nowadays, more and more studies are being made on extraction of antimicrobial agents from plants, and therefore, there will be a larger operating space at the time of selection and use.
The plant extract antibacterial agent is not easy to generate drug resistance, can not induce bacteria and viruses and the like to evolve into a state that the bacteria and viruses are more difficult to resist and eliminate, and solves the problem that the drug resistance of the antibacterial agent is synthesized by people puzzled at present. In addition, compared with the artificially synthesized antibacterial agent, the plant extract antibacterial agent is more natural and environment-friendly, and accords with the pursuit of people in the current age for natural health, environment protection and sustainability.
The electrostatic spinning technology (electrospinning technology) is a novel technology for preparing one-dimensional nano materials. As early as 1934, formhals applied a patent on an experimental set-up for preparing polymer fibers using a high voltage electrostatic field. Later, the development of the technology has been slow due to problems such as low production efficiency. Until the 90 s of the 20 th century, the electrospinning technology was not rapidly developed due to the rise of nanoscience and the push of the task group of the university of acle (Universityof Akron) Darrell h.reneker, the gregoriy c.rutliege, et al, of the millboard company (Massachusetts Institute ofTechnology). The electrostatic spinning manufacturing equipment has simple structure and low spinning cost, can prepare continuous organic, inorganic, organic/inorganic composite, hollow or solid nano-fiber and the like, and is widely paid attention to by researchers. Over the last decade researchers have developed coaxial electrospinning techniques for preparing hollow fibers/nanotubes by improving needle structures or by improving receiving devices, a series of patterned nanofiber structures with different stacking means, such as: ANFs aligned axially along the fiber, nanofibers aligned axially perpendicular to the radial direction, etc. Highly oriented and regularly aligned nanofibers have received great attention from researchers due to their special mechanical, optical and electrical properties.
The basic principle of the electrostatic spinning for realizing the shaping design of the template is to utilize charge concentration and the tip discharge effect of a carrier, wherein the template structure design and the material have direct influence on the obtaining effect of the specific morphology. By changing the shape, material properties and motion state of the receiving templates, various aggregation forms of nanofiber felt materials can be obtained.
Disclosure of Invention
Based on the above prior art, a primary object of the present invention is to provide a method for preparing an antibacterial zein dressing with controllable orientation.
Another object of the present invention is to provide an antibacterial zein dressing with controlled orientation prepared by the above method.
The invention aims at realizing the following technical scheme:
a method for preparing an antibacterial zein dressing with controllable orientation, comprising the following preparation steps:
(1) Dissolving zein in a solvent to obtain a uniform zein solution, adding antibacterial active ingredients, stirring and mixing uniformly, and standing for deaeration to obtain a zein solution containing the antibacterial ingredients;
(2) Respectively preparing a fiber dressing layer by taking a zein solution containing an antibacterial component as a receiver by using a grid template with a geometric figure which is regularly arranged through an electrostatic spinning method, and receiving by a roller receiver with a surface with a stripe and bulge structure which is regularly arranged to obtain a fiber diversion layer; laminating and compounding the fiber dressing layer and the fiber guide layer to obtain a zein fiber membrane functional layer;
(3) The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polyethylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained.
Preferably, the solvent in the step (1) is ethanol or glacial acetic acid aqueous solution with the mass concentration of 40-98%.
Preferably, the mass concentration of zein in the zein solution in the step (1) is 5% -40%.
Preferably, the antimicrobial active includes, but is not limited to, antimicrobial active based on microbial or plant extracts; including but not limited to natural antibacterial extracts such as polylysine, paeonol, tea polyphenols, eucalyptus oil, rose essential oil, aloe extract, honeysuckle extract, baicalin, ginkgo antibacterial protein, lemon grass essential oil, chufa peel extract, etc.
Preferably, the antibacterial active ingredient is added in an amount of 0.01% -5% of the zein mass.
Preferably, the grid templates with the regular geometric figures are hexagonal, rectangular, diamond-shaped, square or round grid templates, the grid size is 0.1-3 mm, and the density is 2-40 pieces/cm 2 The thickness is 0.1-2.0 mm.
Preferably, the roller receiver with the surface provided with the regular stripe and convex structures is a cylindrical roller with the diameter of 8-50 cm and the length of 10-90 cm; the stripe width d of the stripe and convex structure on the surface of the roller is 0.3-1 cm, the density is 1-5 pieces/cm (namely, the interval between the adjacent stripe and convex structures is 0.2-1 cm), the height h of the protrusion is 0.2-1 cm, and the angle alpha of the protrusion is 100-150 degrees. The structure of which is schematically shown in figure 1.
Preferably, the material of the fiber dressing layer receiver comprises plastics, ceramics, metals or alloys; the material of the roller receiver comprises metal or alloy.
Preferably, the electrostatic spinning method comprises needle electrostatic spinning, slit electrostatic spinning, needle-free surface wire electrode electrostatic spinning, needle-free surface roller electrode electrostatic spinning, needle-free surface shuttle electrode electrostatic spinning, needle-free surface spiral wire electrode electrostatic spinning, centrifugal electrostatic spinning or the like.
Preferably, the fiber diameter in the zein fiber membrane functional layer is 50-2000 nm.
An antibacterial zein dressing with controllable orientation is prepared by the method.
The principle of the invention is as follows: the grid template with the geometric figures regularly arranged on the surface is used as a receiver, the arrangement of the figures influences the space arrangement of electrostatic fields, the electric field of the grid cavity part is weakened, and the fiber deposition is less, so that the structure of the fiber membrane is similar to the figure of the template, a large number of micropore structures are distributed, and the air permeability of the dressing is increased; the fiber guide layer is prepared by adopting a roller receiver with a surface with a regular stripe-shaped protruding structure, the rotating roller pulls fibers, so that the fibers form an orientation structure in the circumferential direction or along the polygonal angular direction, the protruding structure on the roller enables the zein fiber membrane to obtain fiber grooves with a complementary structure, the zein fibers are poor in hydrophilicity, liquid flows along the fiber orientation direction after being diffused into the guide layer and is collected in the fiber grooves, the sample has certain temporary liquid storage capacity, meanwhile, the collected liquid flows in the grooves and is diffused outwards, the liquid is prevented from flowing back into wounds again, and the wounds are kept dry.
The preparation method of the invention and the obtained dressing have the following advantages and beneficial effects:
(1) The antibacterial zein dressing with controllable orientation is added with natural antibacterial active ingredients, has good antibacterial effect and no toxic or side effect, and has the antibacterial rate of more than 99 percent on gram-positive bacteria and gram-negative bacteria.
(2) The antibacterial zein dressing with controllable orientation adopts zein with good biocompatibility and biodegradability as a main material, and is environment-friendly, economical and environment-friendly.
(3) The invention adopts electrostatic spinning or centrifugal spinning technology to obtain the composite membrane, the obtained composite membrane has high porosity and large specific surface area, and the fiber structure of the composite membrane has good similarity with extracellular matrix, and the composite membrane can be used as a dressing for skin recovery and regeneration after skin injury or operation.
(4) The microporous structure in the fiber dressing layer obtained by the preparation method has good permeability or air permeability to air and water vapor, but has higher hydrostatic pressure resistance to liquid water permeation, namely the air permeable structure comprises a plurality of openings or channels which can pass through the film from one surface to the other surface, and the size of the holes can enable air and water vapor molecules to pass through the film, so that the porous structure has good resistance to liquid water molecules; and the addition of one or more antibacterial agents enables the antibacterial agent to have good antibacterial activity, so that the antibacterial agent can be released under certain conditions and can be used as a dressing material.
(5) The dressing of the invention gradually enhances the moisture absorption performance from the fiber dressing layer to the spunlaced fabric supporting layer, and forms a good moisture-conducting gradient. Under the action of differential capillary effect, directional water guiding is realized. A dressing with a well-oriented baffle layer is able to achieve a stable balance between penetration and diffusion. When wound exudates or wound pus pass through the fiber membrane functional layer to reach the guide layer, the guide layer has a fluffy structure with a certain thickness and larger longitudinal fiber distribution, so that the exudates or the pus can be quickly captured and longitudinally spread, liquid slowly and effectively enters the guide layer, the liquid is temporarily stored, rewet caused by the fact that the liquid is not absorbed in time is avoided, and dryness of the wound is kept.
Drawings
FIG. 1 is a schematic view of the structure of the stripe-convex structure on the surface of the drum of the present invention;
FIG. 2 is a schematic diagram of the laminate structure of a dressing according to an embodiment of the present invention;
FIG. 3 is a schematic view showing the structure of the fiber dressing layer and the fiber guiding layer in the dressing according to example 1 of the present invention;
FIG. 4 is a schematic view showing the structure of the fiber dressing layer and the fiber guiding layer in the dressing according to example 2 of the present invention;
FIG. 5 is a schematic view showing the structure of a fiber dressing layer and a fiber guiding layer in the dressing according to example 3 of the present invention;
the numbering in the figures is as follows: a 1-polypropylene film barrier layer, a 2-spunlaced fabric supporting layer, a 3-fiber diversion layer and a 4-fiber dressing layer.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but embodiments of the present invention are not limited thereto.
Example 1
Taking analytically pure zein powder particles, selecting an ethanol solution with the mass concentration of 40% as a solvent, and stirring for 30min at normal temperature to obtain a zein solution with the mass concentration of 5% uniformly; after the zein solution is cooled to room temperature, a certain amount of aloe antibacterial extract is weighed and added into the zein solution, and the mixture is stirred for 1h at normal temperature to obtain zein-plant antibacterial extract solution with the aloe extract mass percent of 0.1%, and the mixture is left to stand and defoam for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a free surface electrode electrostatic spinning method. Electrospinning the zein-plant antibacterial extract solution under the voltage of 65kV at the rotating speed of a wire electrode of 10r/min, receiving at a position about 10cm away from a needle, and selecting a linear metal convex roller receiver with the diameter of 50cm, the length of 90cm and the rotating speed of 1200r/min, wherein the surface of the linear metal convex roller receiver is provided with the width of 1cm, the density of 5 pieces/cm, the height of 1cm and the convex angle of 120 DEG to receive the available fiber guide layer; the surface is selected to have the size of 0.5mm and the density of 40 pieces/cm 2 A circular grid pattern receiver of 0.1mm thickness receives the available fibrous dressing layer, the resulting fibrous dressing layer and fibrous deflector layer being schematically illustrated in fig. 3. And (3) laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The obtained zeinThe fiber diameter of the fiber membrane functional layer is 900-1200 nm. The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polypropylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
Antibacterial rate test of the antibacterial zein dressing obtained in this example:
agar was solidified by taking (10.+ -. 0.1) ml of agar in each sterile petri dish. Taking the agar with the required quantity, and heating in water bath to (45+/-1) DEG C. The concentration of the bacterial liquid is (1-5 multiplied by 10) 8 cfu/ml) was inoculated on 150ml agar and the vessel was vigorously shaken to disperse the bacteria evenly. Pour (5.+ -. 0.1) ml onto the dish and allow the agar to solidify. The test was performed using nutrient agar dishes with an inoculation time of one hour. Operating according to EN ISO 20645:2004. The antibacterial zein dressing with a certain area is paved in the middle of the culture by using a sterilizing forceps, so that the fiber dressing layer is well contacted with bacteria, and living bacteria are counted after the culture is carried out for 18 hours to 24 hours at the temperature of (37+/-1). The number of viable bacteria was determined according to EN ISO 20645:2004, yielding an antibacterial zein dressing of this example with an antibacterial rate of 99.67%.
Example 2
Taking analytically pure zein powder particles, selecting an ethanol solution with the mass concentration of 65% as a solvent, and stirring for 30min at normal temperature to obtain a zein solution with the mass concentration of 18% uniformly; after the zein solution is cooled to room temperature, a certain amount of paeonol is weighed and added into the zein solution, and stirring is carried out for 1h at normal temperature, so as to obtain zein-plant antibacterial extract solution with the mass percent of paeonol of 0.4%, and standing and defoaming are carried out for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a needle electrostatic spinning method. Electrospinning zein-plant antibacterial extract solution at a flow rate of 0.4 ml/h/hole under 9kV, and receiving at a position about 10cm from needle, wherein the zein-plant antibacterial extract solution has diameter of 9cm, length of 60cm, and rotation speed of 1300r/min, and the surface hasA roller receiver with a width of 0.5cm, a density of 5 pieces/cm, a height of 0.2cm and a projection angle of 120 DEG is used for receiving the obtainable fiber guide layer; the surface is selected to have the size of 1mm and the density of 30 pieces/cm 2 A square grid pattern receiver with a thickness of 0.5mm receives the available fibrous dressing layer, and a schematic of the structure of the resulting fibrous dressing layer and fibrous deflector layer is shown in fig. 4. And (3) laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The fiber diameter of the functional layer of the zein fiber membrane is about 800-1500 nm. The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polypropylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
The antibacterial zein dressing obtained in the embodiment has an antibacterial rate of 99.95% after test.
Example 3
Taking analytically pure zein powder particles, selecting glacial acetic acid solution with the mass concentration of 90% as a solvent, and stirring for 30min at normal temperature to obtain zein solution with the mass concentration of 19% uniformly; after the zein solution is cooled to room temperature, a certain amount of eucalyptus oil is weighed and added into the zein solution, and the mixture is stirred for 1h at normal temperature to obtain zein-plant antibacterial extract solution with the mass percent of the eucalyptus oil being 0.01%, and the zein-plant antibacterial extract solution is kept stand and defoamed for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a needle-free surface roller electrode electrostatic spinning method. Electrospinning zein-plant antibacterial extract solution at a flow rate of 0.4ml/h under a voltage of 70kV, and receiving at a position about 10cm away from the needle, wherein a roller receiver with a diameter of 40cm, a length of 10cm and a rotating speed of 1500r/min and a linear metal protrusion with a width of 0.3cm, a density of 1cm, a height of 0.5cm and a protrusion angle of 150 DEG is selected for receiving the available fiber guide layer; the surface is selected to have the size of 0.5mm and the density of 30 pieces/cm 2 Thickness of (a)A schematic of the structure of the resulting fibrous dressing layer and fibrous deflector layer is shown in fig. 5 for a 10mm regular hexagonal grid pattern receiver to receive the resulting fibrous dressing layer. And (3) laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The fiber diameter of the functional layer of the zein fiber membrane is 1200-2000 nm. The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polypropylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
The antibacterial zein dressing obtained in the embodiment has an antibacterial rate of 99.69% after test.
Example 4
Taking analytically pure zein powder particles, selecting glacial acetic acid solution with the mass concentration of 98% as a solvent, and stirring for 30min at normal temperature to obtain zein solution with the mass concentration of 20% uniformly; after the zein solution is cooled to room temperature, a certain amount of tea polyphenol is weighed and added into the zein solution, and the mixture is stirred for 1h at normal temperature to obtain zein-plant antibacterial extract solution with the mass percent of the tea polyphenol of 5 percent, and the zein-plant antibacterial extract solution is kept stand and defoamed for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a needle-free surface spiral electrode electrostatic spinning method. Electrospinning zein-plant antibacterial extract solution at a flow rate of 1.2ml/h under a voltage of 60kV, and receiving at a position about 10cm away from the needle, wherein a roller receiver with a diameter of 13cm, a length of 60cm and a rotating speed of 1600r/min and a linear metal protrusion with a width of 1cm, a density of 2 pieces/cm, a height of 1cm and a protrusion angle of 100 DEG on the surface is selected to receive the available fiber guide layer; the surface is selected to have the size of 1mm and the density of 40 pieces/cm 2 And receiving the available fiber dressing layer by a circular grid template receiver with the thickness of 5mm, and laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The diameter of the fiber in the functional layer of the obtained zein fiber membrane is700-1300 nm. The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polypropylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
The antibacterial zein dressing obtained in the embodiment has an antibacterial rate of 99.99% through test.
Example 5
Taking analytically pure zein powder particles, selecting an ethanol solution with the mass concentration of 98% as a solvent, and stirring for 30min at normal temperature to obtain a zein solution with the mass concentration of 40% uniformly; after the zein solution is cooled to room temperature, a certain amount of polylysine is weighed and added into the zein solution, and the mixture is stirred for 1h at normal temperature to obtain zein-plant antibacterial extract solution with the mass percent of polylysine of 3 percent, and the zein-plant antibacterial extract solution is kept stand and defoamed for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a centrifugal electrostatic spinning method. Electrospinning zein-plant antibacterial extract solution at a flow rate of 2.5ml/h under a voltage of 35kV, wherein the rotation speed of a centrifugal electrostatic spinning centrifugal disc is 300r/min, the zein-plant antibacterial extract solution is received at a position about 10cm away from a needle, a roller receiver with a diameter of 8cm, a length of 50cm and a rotating speed of 1500r/min and a linear metal protrusion with a width of 0.8cm, a density of 1cm, a height of 1cm and a protrusion angle of 110 DEG is selected to receive the available fiber guide layer; the surface is selected to have the size of 3mm and the density of 2 pieces/cm 2 And receiving the available fiber dressing layer by a round grid template receiver with the thickness of 20mm, and laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The fiber diameter of the functional layer of the zein fiber membrane is 1000-1500 nm. Compounding a zein fiber membrane functional layer with a spunlaced fabric supporting layer and a polypropylene film barrier layer, wherein the fiber dressing layer, the fiber guide layer, the spunlaced fabric supporting layer and the polypropylene film barrier layer are sequentially arranged from top to bottom to obtain the antibacterial zein with controllable orientationAnd (3) dressing. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
The antibacterial zein dressing obtained in the embodiment has an antibacterial rate of 99.98% after test.
Example 6
Taking analytically pure zein powder particles, selecting glacial acetic acid solution with the mass concentration of 90% as a solvent, and stirring for 30min at normal temperature to obtain zein solution with the mass concentration of 25% uniformly; after the zein solution is cooled to room temperature, a certain amount of aloe antibacterial extract is weighed and added into the zein solution, and the mixture is stirred for 1h at normal temperature to obtain zein-plant antibacterial extract solution with the mass percent of the aloe antibacterial extract of 2 percent, and the zein-plant antibacterial extract solution is kept stand and defoamed for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a needle-free surface shuttle electrode electrostatic spinning method. Electrospinning zein-plant antibacterial extract solution at a flow rate of 3.0ml/h under a voltage of 10kV, and receiving at a position about 10cm away from the needle, wherein a roller receiver with a diameter of 12cm, a diameter of 80cm and a rotating speed of 1400r/min and a linear metal protrusion with a width of 0.3cm, a density of 2 pieces/cm, a height of 0.4cm and a protrusion angle of 125 DEG is selected for receiving the available fiber guide layer; the surface is selected to have the size of 2mm and the density of 20 pieces/cm 2 And receiving the available fiber dressing layer by a diamond grid template receiver with the thickness of 8mm, and laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The fiber diameter of the functional layer of the zein fiber membrane is 1000-1200 nm. The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polypropylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
The antibacterial zein dressing obtained in the embodiment has an antibacterial rate of 99.93% through test.
Example 7
Taking analytically pure jadeThe zein powder particles are prepared by selecting ethanol solution with the mass concentration of 95% as a solvent, and stirring for 30min at normal temperature to obtain zein solution with the mass concentration of 25% uniformly; after the zein solution is cooled to room temperature, a certain amount of aloe and the composite antibacterial extract according to the tree oil (mass ratio of 1:2) are weighed and added into the zein solution, and stirring is carried out for 1h at normal temperature, so as to obtain a zein-plant antibacterial extract solution with the composite antibacterial extract mass percent of 1.0%, and standing and defoaming are carried out for 1h. And preparing the zein-plant extract fiber guide layer and the fiber dressing layer from the prepared mixed solution by adopting a needle electrostatic spinning method. Electrospinning zein-plant antibacterial extract solution at a flow rate of 0.4 ml/h/hole under 13kV voltage, and receiving at a position about 10cm away from the needle, wherein a roller receiver with a diameter of 20cm, a length of 60cm and a rotating speed of 1800r/min and a linear metal protrusion with a width of 1cm, a density of 1cm, a height of 0.5cm and a protrusion angle of 135 DEG on the surface is selected to receive the available fiber guide layer; the surface is selected to have the size of 1mm and the density of 30 pieces/cm 2 And receiving the available fiber dressing layer by a square grid template receiver with the thickness of 0.5mm, and laminating and compositing the fiber dressing layer and the fiber diversion layer to obtain the zein fiber membrane functional layer. The diameter of the fiber in the functional layer of the zein fiber membrane is 50-1000 nm. The zein fiber membrane functional layer is compounded with the water-jet cloth supporting layer and the polypropylene film blocking layer, and the fiber dressing layer, the fiber guide layer, the water-jet cloth supporting layer and the polypropylene film blocking layer are sequentially arranged from top to bottom, so that the antibacterial zein dressing with controllable orientation is obtained. A schematic of the laminate structure of the resulting dressing is shown in fig. 2.
The antibacterial zein dressing obtained in the embodiment has an antibacterial rate of 99.99% through test.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (6)
1. A method for preparing an antibacterial zein dressing with controllable orientation, which is characterized by comprising the following preparation steps:
(1) Dissolving zein in a solvent to obtain a uniform zein solution, adding an antibacterial active ingredient, stirring and mixing uniformly, and standing for deaeration to obtain a zein solution containing the antibacterial ingredient;
(2) Respectively preparing a fiber dressing layer by taking a zein solution containing an antibacterial component as a receiver by using a grid template with a geometric figure which is regularly arranged through an electrostatic spinning method, and receiving by a roller receiver with a surface with a stripe and bulge structure which is regularly arranged to obtain a fiber diversion layer; laminating and compounding the fiber dressing layer and the fiber guide layer to obtain a zein fiber membrane functional layer;
(3) Compounding a zein fiber membrane functional layer with a spunlaced fabric supporting layer and a polypropylene film barrier layer, wherein a fiber dressing layer, a fiber guide layer, the spunlaced fabric supporting layer and a polyethylene film barrier layer are sequentially arranged from top to bottom to obtain an antibacterial zein dressing with controllable orientation;
the grid templates with the regular geometric patterns are hexagonal, square or round grid templates, the size of the grid is 0.1-3 mm, and the density is 2-40 pieces/cm 2 The thickness is 0.1-2.0 mm;
the roller receiver with the surface provided with the regular stripe and bulge structures is a cylindrical roller with the diameter of 8-15 cm and the length of 15-30 cm; the stripe width of the stripe convex structure on the surface of the roller is 0.3-1 cm, the density is 1-5 pieces/cm, the height of the protrusion is 0.2-1 cm, and the angle of the protrusion is 100-150 degrees;
the solvent in the step (1) is ethanol or glacial acetic acid aqueous solution with the mass concentration of 40-98%;
the addition amount of the antibacterial active ingredient is 0.1-5% of the mass of the zein solution.
2. A method of preparing an antimicrobial zein dressing with controlled orientation according to claim 1, wherein: the mass concentration of zein in the zein solution in the step (1) is 5-40%.
3. A method of preparing an antimicrobial zein dressing with controlled orientation according to claim 1, wherein: the antibacterial active ingredient comprises polylysine, paeonol, tea polyphenols, oleum Eucalypti, oleum Rosae Rugosae, aloe extract, flos Lonicerae extract, baicalin, antibacterial protein of semen Ginkgo, lemon grass essential oil or corm Eleocharitis extract.
4. A method of preparing an antimicrobial zein dressing with controlled orientation according to claim 1, wherein: the electrostatic spinning method comprises single needle electrostatic spinning, slit electrostatic spinning, needle-free surface line electrode electrostatic spinning, needle-free surface roller electrode electrostatic spinning, needle-free surface shuttle electrode electrostatic spinning, needle-free surface spiral line electrode electrostatic spinning or centrifugal electrostatic spinning.
5. A method of preparing an antimicrobial zein dressing with controlled orientation according to claim 1, wherein: the diameter of the fiber in the zein fiber membrane functional layer is 50-2000 nm.
6. An antimicrobial zein dressing having a controlled orientation, characterized by: is prepared by the method of any one of claims 1 to 5.
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| CN110025817A (en) * | 2019-04-19 | 2019-07-19 | 青岛大学 | A kind of preparation and application of zein/plants essential oil composite antibacterial fibre dressing |
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| CN111850837A (en) * | 2020-07-24 | 2020-10-30 | 吉林农业大学 | Zein-based uniaxial electrostatic spinning oriented fiber film and preparation method thereof |
| CN112376124A (en) * | 2020-11-20 | 2021-02-19 | 上海市第六人民医院 | Antibacterial dressing |
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| CN112587710A (en) * | 2021-02-05 | 2021-04-02 | 甘肃省分析测试中心 | Preparation process and application of peach kernel extract core-shell nano-structure film |
| CN113417074A (en) * | 2021-05-24 | 2021-09-21 | 江苏省农业科学院 | Preparation method of antibacterial nanofiber membrane |
| CN114752091A (en) * | 2022-03-25 | 2022-07-15 | 中国科学院兰州化学物理研究所 | Preparation method of zein/honeysuckle extract composite antibacterial preservative film |
| CN115531595B (en) * | 2022-09-28 | 2023-08-15 | 长春工业大学 | Antibacterial healing-promoting electrostatic spinning wound dressing and preparation method thereof |
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