CN107155557A - Improve the implantation methods of non-irrigated lotus root yield - Google Patents

Improve the implantation methods of non-irrigated lotus root yield Download PDF

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CN107155557A
CN107155557A CN201710299016.0A CN201710299016A CN107155557A CN 107155557 A CN107155557 A CN 107155557A CN 201710299016 A CN201710299016 A CN 201710299016A CN 107155557 A CN107155557 A CN 107155557A
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parts
lotus root
water
irrigated lotus
root
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零胜
陆坛锋
蓝华云
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Six Directions Zhi Jia Processing Of Farm Products Factory Of Masan County Of Guangxi
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Six Directions Zhi Jia Processing Of Farm Products Factory Of Masan County Of Guangxi
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of implantation methods for improving non-irrigated lotus root yield, including:Seed selection breeding, the non-irrigated lotus root underground stem tuber of selection is soaked using dandelion extract solution, then carries out tissue cultures and obtains non-irrigated lotus root tissue-cultured seedling;Soil is selected;Site preparation is colonized;Field management, is earthed up twice after field planting to seedling strain root;Water and fertilizer management, liquid manure is applied when earthing up twice in non-irrigated lotus root root, and liquid manure includes following raw material:Urea, carbon ammonium, potassium chloride, ammonium phosphate, potassium nitrate, calcium nitrate, magnesium sulfate, zinc chloride, sodium molybdate, ammonium molybdate, boric acid, salicylic acid, bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata, wine, arabite, ribitol, dulcitol, glycerine, fatty acid methyl ester ethoxylate sulfonate and water;And the prevention and control of plant diseases, pest control.The implantation methods of the present invention can effectively improve non-irrigated lotus root yield, with higher actual application value.

Description

Improve the implantation methods of non-irrigated lotus root yield
Technical field
The present invention relates to non-irrigated lotus root implantation methods field, specifically a kind of implantation methods for improving non-irrigated lotus root yield.
Background technology
Non-irrigated lotus root, scientific name queensland arrowrood belongs to Cannaceae, originates in America subtropical zone, herbaceos perennial, suitable for height Warm rain-belt growth, can also be planted in colder high and cold mountain area, be a kind of amylum crops slightly given birth to.Non-irrigated lotus root vitality pole By force, adaptability is wider, and China various regions have general without using agricultural chemicals in plantation, cultivation, are really green without any pollution Color health food.Non-irrigated lotus root is in addition to ornamental plant, feed is made, because its stem root is rich in amylopectin, fresh taro yield it is general 20%~ 25%, dry plate yield is the quality raw materials for producing alcohol fuel, processing and eating and medical starch, utilizes its stem up to more than 60% The starch that root is processed has extensive use in terms of food, medicine.Recently as the quickening of new countryside construction process, part The non-irrigated lotus root of small towns peasant household scale plantation, in addition to beautifying the environment, uses stem root processing and eating starch, cauline leaf is used for herding development and raised after harvesting Material, economic benefit and social benefit are considerable.The development of non-irrigated lotus root industry, to transforming agricultural production, uses Modern management shape Formula is pushed forward the industrialized operation of agriculture, and promotes increasing peasant income to have very great meaning, the plantation of specification and the management of science, is to improve The key of non-irrigated lotus root yield.And the non-irrigated lotus root yield planted according to traditional implantation methods is all not high enough, the reason for non-irrigated lotus root yield is not high It is varied, it is typically all because utilization rate of fertilizer is low and causes, in this case, it is necessary to rational plantation side Method.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least will be described later excellent Point.
It is a still further object of the present invention to provide a kind of implantation methods for improving non-irrigated lotus root yield.It passes through seed selection breeding, soil Ground selection, site preparation field planting, field management, water and fertilizer management and the prevention and control of plant diseases, pest control, effectively improve non-irrigated lotus root yield, with higher Actual application value.
In order to realize that there is provided a kind of plantation side for improving non-irrigated lotus root yield according to object of the present invention and further advantage Method, comprises the following steps:
S1, seed selection breeding:Selection is loose, root is more, complete sturdy, no disease and pests harm the non-irrigated lotus root underground block of fresh full, seed bud Stem, cuts the small stem block with bud from the stem tuber of underground;Small stem block at a temperature of 30~40 DEG C is soaked 2 using dandelion extract solution ~3h, then grows to 4~6cm in being cultivated in sand bed to the bud of small stem block by small stem block;Bud is cut into 2~4mm segment, Then sterilized with volume fraction for 95% ethanol after 10~12s, with aseptic water washing 2~3 times, then with 0.1% mercuric chloride water general Segment sterilizes 6~8s, with aseptic water washing 4~5 times, the explant sterilized;Explant is inoculated on culture medium, in 25 Darkroom is carried out in~35 DEG C of culturing room to cultivate 5~7 days, then differentiation culture, Multiplying culture and culture of rootage are carried out to it, is formed Tissue-cultured seedling, treats that tissue-cultured seedling grows into 7~9cm, obtains non-irrigated lotus root tissue-cultured seedling;
Wherein, the dandelion extract solution is made up of following parts by weight component:Fresh 30~40 parts of dandelion, fresh copper 300~500 parts of 10~15 parts of algae, 8~10 parts of fresh sea lettuce, 20~25 parts of ethyl acetate and sterilized water;By above-mentioned parts by weight Several fresh dandelion, fresh Sargassum horneri, the mixing of fresh sea lettuce are mashed, and then add the ethyl acetate of above-mentioned parts by weight and sterile Water, in 30~35min of ultrasound under 60~80KHz frequencies, ultrasonic temperature controls at 25~30 DEG C, is then filtered to remove filter residue, takes Filtrate centrifugal treating, then take supernatant liquor, that is, the dandelion extract solution is made;
S2, soil selection:Select illumination sufficient, day and night temperature is big, irrigation and drainage are convenient, soil layer is deep, loose fertile, it is neutral or Meta-acid and the sand soil rich in organic matter and humus;
S3, site preparation field planting:First 30~40 days of plantation, by ploughing soil 1 time, applies general calcium, in the sun according to 20kg/ mus Turned over again after shining 5 days, general calcium is applied according to 30kg/ mus, after shining 10 days in the sun, carried out third time and turn over, mu is applied organic Fertile 2800kg carries out site preparation, thin rake of deep ploughing, and soil is broken to turn up soil carefully, plays furrow by north-south and waits to plant;In temperature is stable 15 in the daytime after the beginning of spring At~18 DEG C, carry out digging the non-irrigated lotus root tissue-cultured seedling of cave field planting away from 60~70cm with 70~80cm of line-spacing, row in the furrow ditch held successfully;
S4, field management:Seedling is looked into the progress of non-irrigated lotus root tissue-cultured seedling and filled a vacancy within 10~15 days after field planting;After field planting 20~30 days to seedling Strain root carries out first time ridging;Second is carried out to seedling strain root and is earthed up within 30~40 days after earthing up for the first time;Non-irrigated lotus root Post flowering Flower shears is gone immediately;
S5, water and fertilizer management:200~250g of liquid manure is applied at away from non-irrigated 3~5cm of lotus root root when earthing up for the first time, second of training When native 550~600g of liquid manure is applied at away from non-irrigated 8~10cm of lotus root root;The liquid manure includes the raw material of following parts by weight:Urea 15~25 parts, 10~15 parts of carbon ammonium, 10~12 parts of potassium chloride, 8~10 parts of ammonium phosphate, 1~3 part of potassium nitrate, 1~3 part of calcium nitrate, 0.2~0.4 part of magnesium sulfate, 0.1~0.3 part of zinc chloride, 0.2~0.4 part of sodium molybdate, 0.1~0.3 part of ammonium molybdate, boric acid 0.1~ 0.3 part, 0.1~0.3 part of salicylic acid, bitter 2~3 parts of lettuce, 2~3 parts of the root bark of shaggy-fruited dittany, 1~2 part of spinach spinach grass, 1~2 part of rheum officinale, Jin Ying 1~2 part of son, 0.5~0.8 part of ginkgo leaf, 0.8~1.0 part of selfheal, 0.5~0.6 part of Sculellaria barbata, 40~50 parts of wine, Arab 4~6 parts of sugar alcohol, 2~4 parts of ribitol, 1~3 part of dulcitol, 1~3 part of glycerine, fatty acid methyl ester ethoxylate sulfonate 0.01~0.03 part and appropriate amount of water;
Wherein, the preparation method of the liquid manure comprises the following steps:
A. according to above-mentioned parts by weight, arabite, ribitol, dulcitol, glycerine are dissolved in the water of 100 parts by weight In sugar alcohol solution, then by magnesium sulfate, zinc chloride, sodium molybdate, ammonium molybdate, boric acid and fatty acid methyl ester ethoxylate sulphur Hydrochlorate adds sugar alcohol solution, and 5~6h is stirred at 90~100 DEG C, obtains sugar alcohol chelating Liquid Fertilizer;
B. according to above-mentioned parts by weight, by bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata and wine are firstly placed in enamel pot, be then placed on it is another bigger be filled with water in pot, pot reclaimed water temperature control system that is filled with water is 40~50 DEG C, then cover after enamel tank cover, 30~36h of water bath with thermostatic control, filter and remove residue obtains Chinese medicine liquid, 50~60 are added in Chinese medicine liquid The water of parts by weight, obtains preventing disease and pest decoction;
C. according to above-mentioned parts by weight, urea, carbon ammonium, potassium chloride, ammonium phosphate are added to the water of 400~500 parts by weight In stir, obtain composite fertilizer's feed liquid;
D. sugar alcohol made from step a is chelated into Liquid Fertilizer, preventing disease and pest decoction made from step b with being answered made from step c Hefei feed liquid mixing, after being sufficiently stirred for, that is, is made the liquid manure;
S6, the prevention and control of plant diseases, pest control:, there is bud rot, stem rot with 75% carbendazol wettable powder 800 in non-irrigated lotus root growth period ~1000 times of liquid are sprayed;There is virosis to be sprayed with 50% moroxydine wettable powder, 800~1000 times of liquid;There is rust to use Clever 500~800 times of liquid of wettable powder of 15% powder rust are sprayed;There is budworm to be sprayed with 50% dimethoate emulsifiable concentrate, 800~900 times of liquid Kill;There is any of several broadleaf plants insect parasite which hurts rice plant to be sprayed with DDVP, 2000~2200 times of liquid of Amitraz compounded cream preparation.
Preferably, the depth that three times are turned in S3 is 30~40cm.
Preferably, in S3 organic fertilizer be dung polluted water and crush after bean dregs, tartary buckwheat shell, chalina, cottonseed cake, orange peel, Water hyacinth and water are according to mass ratio 15:1.4:0.8:1.7:1.2:2.6:1.3:Become thoroughly decomposed and form after 5 mixing.
Preferably, a water was poured after being emerged in S4 every 5~6 days, soil moisture is kept between 60%~70%.
Preferably, the biphosphate for being also 3% in seedling mass fraction of strain foliage-spray when being earthed up for the first time in S4 The mixed solution of potassium and 2% ammonium hydrogen carbonate.
Preferably, bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata are equal in S5 For Fresh Plants.
Preferably, DDVP, Amitraz compounded cream preparation include the raw material and auxiliary material of following parts by weight in S6:Enemy 25~30 parts of dichlorvos active compound, 10~12 parts of Amitraz active compound, 8~10 parts of emulsifying agent OPEO, solvent acetone 90 Part.
The present invention at least includes following beneficial effect:
Firstth, the small stem block of the non-irrigated lotus root with bud is soaked into 2~3h using dandelion extract solution at a temperature of 30~40 DEG C, then Germination, segment, sterilization obtain explant;Explant is subjected to darkroom culture, then carries out differentiation culture, Multiplying culture and training of taking root Support, form tissue-cultured seedling;Dandelion extract solution is total to by fresh dandelion, fresh Sargassum horneri, fresh sea lettuce, ethyl acetate and sterilized water With being made, in dandelion, Sargassum horneri and sea lettuce containing leukotrienes, carvol, furfural, benzaldehyde, citric acid, first fragrant aldehyde, vanillic aldehyde, Cinnaldehydrum, isovanillin, limonene, the brown DME in ocean, to poly- umbrella lemon florigen, branches and leaves element, eugenol, aromatic alcohol, vitamin B12 Etc. a series of materials, cell basal layer cell vigor, anti-oxidant, suppression free radical activity, desalination melanin pigmentation, tool can be strengthened Have functions that to promote cytothesis, increase cell water conservation degree, activation epidermal cell;The small stem of non-irrigated lotus root is soaked using dandelion extract solution Block can prevent explant from occurring brown stain, can promote its merisis, improve the bioactivity of explant;
Secondth, site preparation field planting during in three times to soil carry out plough deeply cultivated Exposure to Sunlight, plough deeply plough can thicken active soil stratum, non-irrigated lotus root Root system is typically distributed across in 10~20cm arable layer, therefore tilth should can thus make active soil stratum thick in 30~40cm Degree be maintained at 10~20cm, break plough sole, be conducive to retain water and nutrients, again will not soil excessively hang void, influence water retention property, also Soil air capacity can be increased, the soil water, gas contradiction are solved well, be conducive to the growth of non-irrigated lotus root seedling strain root system;
3rd, by arabite, ribitol, dulcitol, glycerine it is soluble in water sugar alcohol solution, then by sulfuric acid Magnesium, zinc chloride, sodium molybdate, ammonium molybdate, boric acid and fatty acid methyl ester ethoxylate sulfonate add sugar alcohol solution, 90~ 100 DEG C of 5~6h of stirring, obtain sugar alcohol chelating Liquid Fertilizer;Sugar alcohol is polyol, is photosynthetic head product, due to The trophic function of itself and good metal complexing capability, stable complex can be combined to form with a variety of nutriments, is only One can carry the material that mineral nutrient is transported in plantling strain bast, and its molecular weight is low, it is easy to absorbed by plant, Enter easily to degrade in plant body and discharge nutrient;The sugar alcohol of gained chelates Liquid Fertilizer as the raw material of liquid manure and puts on non-irrigated lotus root Root, is different from spraying on seedling strain blade face for conventional art, non-irrigated lotus root can be made more easily to be absorbed, fertilizer is effectively increased The utilization rate of material, so as to improve the yield of non-irrigated lotus root;
4th, by preventing that bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata are made Pest and disease damage decoction has preferable insect prevention bactericidal effect, for the sickle-like bacteria in soil in planting pit, Pseudomonas solanacearum, anthrax The harmful levels of pathogens such as bacillus carry out sterilization sterilizing, effectively suppress the growth and breeding of harmful levels of pathogens, prevent disease;
5th, DDVP, Amitraz compounded cream preparation have the effect that is mutually improved, and can prevent to delay for any of several broadleaf plants insect parasite which hurts rice plant It develops immunity to drugs.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Embodiment
The present invention is elaborated with reference to embodiment, to make those of ordinary skill in the art refer to after this specification It can implement according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or many The presence or addition of individual other elements or its combination.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute Reagent and material are stated, unless otherwise specified, is commercially obtained.
<Embodiment 1>
A kind of implantation methods for improving non-irrigated lotus root yield, comprise the following steps:
S1, seed selection breeding:Selection is loose, root is more, complete sturdy, no disease and pests harm the non-irrigated lotus root underground block of fresh full, seed bud Stem, cuts the small stem block with bud from the stem tuber of underground;Small stem block is soaked into 2h using dandelion extract solution at a temperature of 30 DEG C, so Small stem block is grown into 4cm in being cultivated in sand bed to the bud of small stem block afterwards;Bud is cut into 2mm segment, volume integral is then used After number is 95% ethanol sterilizing 10s, segment is sterilized 6s with aseptic water washing 2 times, then with 0.1% mercuric chloride water, sterilized water is used Rinse 4 times, the explant sterilized;Explant is inoculated on culture medium, darkroom culture 5 is carried out in 25 DEG C of culturing room My god, then differentiation culture, Multiplying culture and culture of rootage are carried out to it, tissue-cultured seedling is formed, treats that tissue-cultured seedling grows into 7cm, obtains drought Lotus root tissue-cultured seedling;
Wherein, the dandelion extract solution is made up of following parts by weight component:Fresh 30 parts of dandelion, fresh Sargassum horneri 10 Part, 8 parts of fresh sea lettuce, 20 parts of ethyl acetate and 300 parts of sterilized water;By the fresh dandelion of above-mentioned parts by weight, fresh copper Algae, the mixing of fresh sea lettuce are mashed, and then add the ethyl acetate and sterilized water of above-mentioned parts by weight, in ultrasonic under 60KHz frequencies 30min, ultrasonic temperature is controlled at 25 DEG C, is then filtered to remove filter residue, is taken filtrate centrifugal treating, then takes supernatant liquor, that is, is made The dandelion extract solution;
S2, soil selection:Select illumination sufficient, day and night temperature is big, irrigation and drainage are convenient, soil layer is deep, loose fertile, it is neutral or Meta-acid and the sand soil rich in organic matter and humus;
S3, site preparation field planting:First 30 days of plantation, by ploughing soil 1 time, applies general calcium according to 20kg/ mus, 5 is shone in the sun Turned over again after it, general calcium is applied according to 30kg/ mus, after shining 10 days in the sun, carried out third time and turn over, the depth turned over for three times Degree is 30cm, and mu organic fertilizer 2800kg carries out site preparation, thin rake of deep ploughing, and soil is broken to turn up soil carefully, plays furrow by north-south and waits to plant;In the beginning of spring Temperature is stable at 15 DEG C in the daytime afterwards, carries out digging the non-irrigated lotus root group of cave field planting away from 60cm with line-spacing 70cm, row in the furrow ditch held successfully Train seedling;The organic fertilizer is bean dregs, tartary buckwheat shell, chalina, cottonseed cake, orange peel, water hyacinth and the water after dung polluted water and crushing According to mass ratio 15:1.4:0.8:1.7:1.2:2.6:1.3:Become thoroughly decomposed and form after 5 mixing;
S4, field management:A water was poured after emerging every 5 days, soil moisture is kept 60%;After field planting 10 days to non-irrigated lotus root Tissue-cultured seedling progress is looked into seedling and filled a vacancy;First time ridging was carried out to seedling strain root in 20 days after field planting, while in seedling strain foliage-spray once Mass fraction is 3% potassium dihydrogen phosphate and the mixed solution of 2% ammonium hydrogen carbonate;For the first time earth up after 30 days to seedling strain root Second is carried out to earth up;Non-irrigated lotus root Post flowering immediately removes flower shears;
S5, water and fertilizer management:Apply liquid manure 200g at away from non-irrigated lotus root root 3cm when earthing up for the first time, second when earthing up in away from Liquid manure 550g is applied at non-irrigated lotus root root 8cm;The liquid manure includes the raw material of following parts by weight:15 parts of urea, 10 parts of carbon ammonium, chlorination 10 parts of potassium, 8 parts of ammonium phosphate, 1 part of potassium nitrate, 1 part of calcium nitrate, 0.2 part of magnesium sulfate, 0.1 part of zinc chloride, 0.2 part of sodium molybdate, molybdic acid 0.1 part of ammonium, 0.1 part of boric acid, 0.1 part of salicylic acid, bitter 2 parts of lettuce, 2 parts of the root bark of shaggy-fruited dittany, 1 part of spinach spinach grass, 1 part of rheum officinale, the fruit of Cherokee rose 1 Part, 0.5 part of ginkgo leaf, 0.8 part of selfheal, 0.5 part of Sculellaria barbata, 40 parts of wine, 4 parts of arabite, 2 parts of ribitol, dulcitol 1 Part, 1 part of glycerine, 0.01 part of fatty acid methyl ester ethoxylate sulfonate and appropriate amount of water;
Wherein, bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata are fresh plant Strain;The preparation method of the liquid manure comprises the following steps:
A. according to above-mentioned parts by weight, arabite, ribitol, dulcitol, glycerine are dissolved in the water of 100 parts by weight In sugar alcohol solution, then by magnesium sulfate, zinc chloride, sodium molybdate, ammonium molybdate, boric acid and fatty acid methyl ester ethoxylate sulphur Hydrochlorate adds sugar alcohol solution, and 5h is stirred at 90 DEG C, obtains sugar alcohol chelating Liquid Fertilizer;
B. according to above-mentioned parts by weight, by bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata and wine are firstly placed in enamel pot, be then placed on it is another bigger be filled with water in pot, pot reclaimed water temperature control system that is filled with water is at 40 DEG C, so After bonnet enameling cover, water bath with thermostatic control 30h, filter and remove residue obtains Chinese medicine liquid, and the water of 50 parts by weight is added in Chinese medicine liquid, Obtain preventing disease and pest decoction;
C. according to above-mentioned parts by weight, urea, carbon ammonium, potassium chloride, ammonium phosphate is added and stirred in the water of 400 parts by weight Uniformly, composite fertilizer's feed liquid is obtained;
D. sugar alcohol made from step a is chelated into Liquid Fertilizer, preventing disease and pest decoction made from step b with being answered made from step c Hefei feed liquid mixing, after being sufficiently stirred for, that is, is made the liquid manure;
S6, the prevention and control of plant diseases, pest control:, there is bud rot, stem rot with 75% carbendazol wettable powder 800 in non-irrigated lotus root growth period Times liquid is sprayed;There is virosis to be sprayed with 50% moroxydine wettable powder, 800 times of liquid;There is rust wettable with the rust spirit of 15% powder Property 500 times of liquid of pulvis are sprayed;There is budworm to be sprayed with 50% dimethoate emulsifiable concentrate, 800 times of liquid;There is any of several broadleaf plants insect parasite which hurts rice plant DDVP, it is double 2000 times of liquid of carbonamidine compounded cream preparation are sprayed, and DDVP, Amitraz compounded cream preparation include following parts by weight Raw material and auxiliary material:25 parts of DDVP active compound, 10 parts of Amitraz active compound, 8 parts of emulsifying agent OPEO, solvent acetone 90 Part.
<Embodiment 2>
A kind of implantation methods for improving non-irrigated lotus root yield, comprise the following steps:
S1, seed selection breeding:Selection is loose, root is more, complete sturdy, no disease and pests harm the non-irrigated lotus root underground block of fresh full, seed bud Stem, cuts the small stem block with bud from the stem tuber of underground;Small stem block is soaked into 3h using dandelion extract solution at a temperature of 40 DEG C, so Small stem block is grown into 6cm in being cultivated in sand bed to the bud of small stem block afterwards;Bud is cut into 4mm segment, volume integral is then used After number is 95% ethanol sterilizing 12s, segment is sterilized 8s with aseptic water washing 3 times, then with 0.1% mercuric chloride water, sterilized water is used Rinse 5 times, the explant sterilized;Explant is inoculated on culture medium, darkroom culture 7 is carried out in 35 DEG C of culturing room My god, then differentiation culture, Multiplying culture and culture of rootage are carried out to it, tissue-cultured seedling is formed, treats that tissue-cultured seedling grows into 9cm, obtains drought Lotus root tissue-cultured seedling;
Wherein, the dandelion extract solution is made up of following parts by weight component:Fresh 40 parts of dandelion, fresh Sargassum horneri 15 Part, 10 parts of fresh sea lettuce, 25 parts of ethyl acetate and 500 parts of sterilized water;By the fresh dandelion of above-mentioned parts by weight, fresh copper Algae, the mixing of fresh sea lettuce are mashed, and then add the ethyl acetate and sterilized water of above-mentioned parts by weight, in ultrasonic under 80KHz frequencies 35min, ultrasonic temperature is controlled at 30 DEG C, is then filtered to remove filter residue, is taken filtrate centrifugal treating, then takes supernatant liquor, that is, is made The dandelion extract solution;
S2, soil selection:Select illumination sufficient, day and night temperature is big, irrigation and drainage are convenient, soil layer is deep, loose fertile, it is neutral or Meta-acid and the sand soil rich in organic matter and humus;
S3, site preparation field planting:First 40 days of plantation, by ploughing soil 1 time, applies general calcium according to 20kg/ mus, 5 is shone in the sun Turned over again after it, general calcium is applied according to 30kg/ mus, after shining 10 days in the sun, carried out third time and turn over, the depth turned over for three times Degree is 40cm, and mu organic fertilizer 2800kg carries out site preparation, thin rake of deep ploughing, and soil is broken to turn up soil carefully, plays furrow by north-south and waits to plant;In the beginning of spring Temperature is stable at 18 DEG C in the daytime afterwards, carries out digging the non-irrigated lotus root group of cave field planting away from 70cm with line-spacing 80cm, row in the furrow ditch held successfully Train seedling;The organic fertilizer is bean dregs, tartary buckwheat shell, chalina, cottonseed cake, orange peel, water hyacinth and the water after dung polluted water and crushing According to mass ratio 15:1.4:0.8:1.7:1.2:2.6:1.3:Become thoroughly decomposed and form after 5 mixing;
S4, field management:A water was poured after emerging every 6 days, soil moisture is kept 70%;After field planting 15 days to non-irrigated lotus root Tissue-cultured seedling progress is looked into seedling and filled a vacancy;First time ridging was carried out to seedling strain root in 30 days after field planting, while in seedling strain foliage-spray once Mass fraction is 3% potassium dihydrogen phosphate and the mixed solution of 2% ammonium hydrogen carbonate;For the first time earth up after 40 days to seedling strain root Second is carried out to earth up;Non-irrigated lotus root Post flowering immediately removes flower shears;
S5, water and fertilizer management:Apply liquid manure 250g at away from non-irrigated lotus root root 5cm when earthing up for the first time, second when earthing up in away from Liquid manure 600g is applied at non-irrigated lotus root root 10cm;The liquid manure includes the raw material of following parts by weight:25 parts of urea, 15 parts of carbon ammonium, chlorine Change 12 parts of potassium, 10 parts of ammonium phosphate, 3 parts of potassium nitrate, 3 parts of calcium nitrate, 0.4 part of magnesium sulfate, 0.3 part of zinc chloride, 0.4 part of sodium molybdate, 0.3 part of ammonium molybdate, 0.3 part of boric acid, 0.3 part of salicylic acid, bitter 3 parts of lettuce, 3 parts of the root bark of shaggy-fruited dittany, 2 parts of spinach spinach grass, 2 parts of rheum officinale, Jin Ying 2 parts of son, 0.8 part of ginkgo leaf, 1.0 parts of selfheal, 0.6 part of Sculellaria barbata, 50 parts of wine, 6 parts of arabite, 4 parts of ribitol, winged euonymus 3 parts of alcohol, 3 parts of glycerine, 0.03 part of fatty acid methyl ester ethoxylate sulfonate and appropriate amount of water;
Wherein, bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata are fresh plant Strain;The preparation method of the liquid manure comprises the following steps:
A. according to above-mentioned parts by weight, arabite, ribitol, dulcitol, glycerine are dissolved in the water of 100 parts by weight In sugar alcohol solution, then by magnesium sulfate, zinc chloride, sodium molybdate, ammonium molybdate, boric acid and fatty acid methyl ester ethoxylate sulphur Hydrochlorate adds sugar alcohol solution, and 6h is stirred at 100 DEG C, obtains sugar alcohol chelating Liquid Fertilizer;
B. according to above-mentioned parts by weight, by bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata and wine are firstly placed in enamel pot, be then placed on it is another bigger be filled with water in pot, pot reclaimed water temperature control system that is filled with water is at 50 DEG C, so After bonnet enameling cover, water bath with thermostatic control 36h, filter and remove residue obtains Chinese medicine liquid, and the water of 60 parts by weight is added in Chinese medicine liquid, Obtain preventing disease and pest decoction;
C. according to above-mentioned parts by weight, urea, carbon ammonium, potassium chloride, ammonium phosphate is added and stirred in the water of 500 parts by weight Uniformly, composite fertilizer's feed liquid is obtained;
D. sugar alcohol made from step a is chelated into Liquid Fertilizer, preventing disease and pest decoction made from step b with being answered made from step c Hefei feed liquid mixing, after being sufficiently stirred for, that is, is made the liquid manure;
S6, the prevention and control of plant diseases, pest control:, there is 75% carbendazol wettable powder of bud rot, stem rot in non-irrigated lotus root growth period 1000 times of liquid are sprayed;There is virosis to be sprayed with 50% moroxydine wettable powder, 1000 times of liquid;There is rust to be become rusty with 15% powder Clever 800 times of liquid of wettable powder are sprayed;There is budworm to be sprayed with 50% dimethoate emulsifiable concentrate, 900 times of liquid;There is any of several broadleaf plants insect parasite which hurts rice plant enemy Dichlorvos, 2200 times of liquid of Amitraz compounded cream preparation are sprayed, and DDVP, Amitraz compounded cream preparation include following weight The raw material and auxiliary material of number:It is 30 parts of DDVP active compound, 12 parts of Amitraz active compound, 10 parts of emulsifying agent OPEO, molten 90 parts of agent acetone.
<Embodiment 3>
A kind of implantation methods for improving non-irrigated lotus root yield, comprise the following steps:
S1, seed selection breeding:Selection is loose, root is more, complete sturdy, no disease and pests harm the non-irrigated lotus root underground block of fresh full, seed bud Stem, cuts the small stem block with bud from the stem tuber of underground;Small stem block at a temperature of 35 DEG C is soaked into 2.5h using dandelion extract solution, Then small stem block is grown into 5cm in being cultivated in sand bed to the bud of small stem block;Bud is cut into 3mm segment, volume is then used After fraction is 95% ethanol sterilizing 11s, segment is sterilized 7s with aseptic water washing 2 times, then with 0.1% mercuric chloride water, with sterile Water is rinsed 4 times, the explant sterilized;Explant is inoculated on culture medium, darkroom training is carried out in 30 DEG C of culturing room Support 6 days, then differentiation culture, Multiplying culture and culture of rootage are carried out to it, form tissue-cultured seedling, treat that tissue-cultured seedling grows into 8cm, obtain Non-irrigated lotus root tissue-cultured seedling;
Wherein, the dandelion extract solution is made up of following parts by weight component:Fresh 35 parts of dandelion, fresh Sargassum horneri 12 Part, 9 parts of fresh sea lettuce, 22 parts of ethyl acetate and 400 parts of sterilized water;By the fresh dandelion of above-mentioned parts by weight, fresh copper Algae, the mixing of fresh sea lettuce are mashed, and then add the ethyl acetate and sterilized water of above-mentioned parts by weight, in ultrasonic under 70KHz frequencies 32min, ultrasonic temperature is controlled at 28 DEG C, is then filtered to remove filter residue, is taken filtrate centrifugal treating, then takes supernatant liquor, that is, is made The dandelion extract solution;
S2, soil selection:Select illumination sufficient, day and night temperature is big, irrigation and drainage are convenient, soil layer is deep, loose fertile, it is neutral or Meta-acid and the sand soil rich in organic matter and humus;
S3, site preparation field planting:First 35 days of plantation, by ploughing soil 1 time, applies general calcium according to 20kg/ mus, 5 is shone in the sun Turned over again after it, general calcium is applied according to 30kg/ mus, after shining 10 days in the sun, carried out third time and turn over, the depth turned over for three times Degree is 35cm, and mu organic fertilizer 2800kg carries out site preparation, thin rake of deep ploughing, and soil is broken to turn up soil carefully, plays furrow by north-south and waits to plant;In the beginning of spring Temperature is stable at 16 DEG C in the daytime afterwards, carries out digging the non-irrigated lotus root group of cave field planting away from 65cm with line-spacing 75cm, row in the furrow ditch held successfully Train seedling;The organic fertilizer is bean dregs, tartary buckwheat shell, chalina, cottonseed cake, orange peel, water hyacinth and the water after dung polluted water and crushing According to mass ratio 15:1.4:0.8:1.7:1.2:2.6:1.3:Become thoroughly decomposed and form after 5 mixing;
S4, field management:A water was poured after emerging every 5 days, soil moisture is kept 65%;After field planting 12 days to non-irrigated lotus root Tissue-cultured seedling progress is looked into seedling and filled a vacancy;First time ridging was carried out to seedling strain root in 25 days after field planting, while in seedling strain foliage-spray once Mass fraction is 3% potassium dihydrogen phosphate and the mixed solution of 2% ammonium hydrogen carbonate;For the first time earth up after 35 days to seedling strain root Second is carried out to earth up;Non-irrigated lotus root Post flowering immediately removes flower shears;
S5, water and fertilizer management:Apply liquid manure 220g at away from non-irrigated lotus root root 4cm when earthing up for the first time, second when earthing up in away from Liquid manure 580g is applied at non-irrigated lotus root root 9cm;The liquid manure includes the raw material of following parts by weight:20 parts of urea, 12 parts of carbon ammonium, chlorination 11 parts of potassium, 9 parts of ammonium phosphate, 2 parts of potassium nitrate, 2 parts of calcium nitrate, 0.3 part of magnesium sulfate, 0.2 part of zinc chloride, 0.3 part of sodium molybdate, molybdic acid 0.2 part of ammonium, 0.2 part of boric acid, 0.2 part of salicylic acid, bitter 2.5 parts of lettuce, 2.5 parts of the root bark of shaggy-fruited dittany, 1.5 parts of spinach spinach grass, 1.5 parts of rheum officinale, 1.5 parts of the fruit of Cherokee rose, 0.6 part of ginkgo leaf, 0.9 part of selfheal, 0.5 part of Sculellaria barbata, 45 parts of wine, 5 parts of arabite, ribitol 3 Part, 2 parts of dulcitol, 2 parts of glycerine, 0.02 part of fatty acid methyl ester ethoxylate sulfonate and appropriate amount of water;
Wherein, bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata are fresh plant Strain;The preparation method of the liquid manure comprises the following steps:
A. according to above-mentioned parts by weight, arabite, ribitol, dulcitol, glycerine are dissolved in the water of 100 parts by weight In sugar alcohol solution, then by magnesium sulfate, zinc chloride, sodium molybdate, ammonium molybdate, boric acid and fatty acid methyl ester ethoxylate sulphur Hydrochlorate adds sugar alcohol solution, and 5.5h is stirred at 95 DEG C, obtains sugar alcohol chelating Liquid Fertilizer;
B. according to above-mentioned parts by weight, by bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata and wine are firstly placed in enamel pot, be then placed on it is another bigger be filled with water in pot, pot reclaimed water temperature control system that is filled with water is at 45 DEG C, so After bonnet enameling cover, water bath with thermostatic control 33h, filter and remove residue obtains Chinese medicine liquid, and the water of 55 parts by weight is added in Chinese medicine liquid, Obtain preventing disease and pest decoction;
C. according to above-mentioned parts by weight, urea, carbon ammonium, potassium chloride, ammonium phosphate is added and stirred in the water of 450 parts by weight Uniformly, composite fertilizer's feed liquid is obtained;
D. sugar alcohol made from step a is chelated into Liquid Fertilizer, preventing disease and pest decoction made from step b with being answered made from step c Hefei feed liquid mixing, after being sufficiently stirred for, that is, is made the liquid manure;
S6, the prevention and control of plant diseases, pest control:, there is bud rot, stem rot with 75% carbendazol wettable powder 900 in non-irrigated lotus root growth period Times liquid is sprayed;There is virosis to be sprayed with 50% moroxydine wettable powder, 900 times of liquid;There is rust wettable with the rust spirit of 15% powder Property 600 times of liquid of pulvis are sprayed;There is budworm to be sprayed with 50% dimethoate emulsifiable concentrate, 850 times of liquid;There is any of several broadleaf plants insect parasite which hurts rice plant DDVP, it is double 2100 times of liquid of carbonamidine compounded cream preparation are sprayed, and DDVP, Amitraz compounded cream preparation include following parts by weight Raw material and auxiliary material:28 parts of DDVP active compound, 11 parts of Amitraz active compound, 9 parts of emulsifying agent OPEO, solvent acetone 90 Part.
<Contrast test>
Non-irrigated lotus root is planted using conventional method, site preparation is turned over before plantation, using common base fertilizer 2800kg, then plants into commercially available Non-irrigated lotus root tissue-cultured seedling, growth period fertilising and the prevention and control of plant diseases, pest control are carried out according still further to conventional method, and the non-irrigated lotus root that the method is planted is as right Ratio 1, is contrasted by the planting survival rates to non-irrigated lotus root, the per mu yield of non-irrigated lotus root and embodiment 1~3, obtains following data:
Each embodiment of table 1 is to the planting survival rates of non-irrigated lotus root, the influence of the per mu yield of non-irrigated lotus root
Comparative example 1 Embodiment 1 Embodiment 2 Embodiment 3
Planting survival rates (%) 86.3 93.2 94.5 95.4
Non-irrigated lotus root per mu yield (%) 2816 3759 3828 3889
As can be seen from Table 1, the non-irrigated lotus root planted by the present invention, survival rate is 93.2%~95.4% after field planting, more than contrast The 86.3% of example 1;The non-irrigated lotus root per mu yield that the present invention is planted is up to 3759~3889kg, higher than the 2816kg of comparative example 1;As can be seen here The implantation methods of the present invention can be significantly increased the yield of non-irrigated lotus root plantation, increase the income of peasant household, improve the non-irrigated lotus root of plantation Economic benefit.
In addition, the non-irrigated lotus root planted of the present invention cooks, rear delicious flavour, mouthfeel be soft, sweet perfume is glutinous, suitable each age group Crowd eats, with good edibility.
Number of devices and treatment scale described herein are the explanations for simplifying the present invention.To the present invention application, Modifications and variations will be readily apparent to persons skilled in the art.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited In specific details and shown here as the embodiment with description.

Claims (7)

1. a kind of implantation methods for improving non-irrigated lotus root yield, it is characterised in that comprise the following steps:
S1, seed selection breeding:Selection is loose, root is more, complete sturdy, no disease and pests harm the non-irrigated lotus root underground stem tuber of fresh full, seed bud, from The small stem block with bud is cut on the stem tuber of underground;Small stem block is soaked into 2~3h using dandelion extract solution at a temperature of 30~40 DEG C, Then small stem block is grown into 4~6cm in being cultivated in sand bed to the bud of small stem block;Bud is cut into 2~4mm segment, then Sterilized with volume fraction for 95% ethanol after 10~12s, with aseptic water washing 2~3 times, then with 0.1% mercuric chloride water by segment Sterilize 6~8s, with aseptic water washing 4~5 times, the explant sterilized;Explant is inoculated on culture medium, in 25~35 DEG C culturing room in carry out darkroom and cultivate 5~7 days, then carry out differentiation culture, Multiplying culture and culture of rootage to it, form tissue culture Seedling, treats that tissue-cultured seedling grows into 7~9cm, obtains non-irrigated lotus root tissue-cultured seedling;
Wherein, the dandelion extract solution is made up of following parts by weight component:Fresh 30~40 parts of dandelion, fresh Sargassum horneri 10 300~500 parts of~15 parts, 8~10 parts of fresh sea lettuce, 20~25 parts of ethyl acetate and sterilized water;By above-mentioned parts by weight Fresh dandelion, fresh Sargassum horneri, the mixing of fresh sea lettuce are mashed, and then add the ethyl acetate and sterilized water of above-mentioned parts by weight, In 30~35min of ultrasound under 60~80KHz frequencies, ultrasonic temperature controls at 25~30 DEG C, is then filtered to remove filter residue, takes filtrate Centrifugal treating, then take supernatant liquor, that is, the dandelion extract solution is made;
S2, soil selection:Select illumination sufficient, day and night temperature is big, irrigation and drainage are convenient, soil layer is deep, loose fertile, neutral or meta-acid And the sand soil rich in organic matter and humus;
S3, site preparation field planting:First 30~40 days of plantation, by ploughing soil 1 time, applies general calcium according to 20kg/ mus, 5 is shone in the sun Turned over again after it, general calcium is applied according to 30kg/ mus, after shining 10 days in the sun, carried out third time and turn over, mu organic fertilizer 2800kg carries out site preparation, thin rake of deep ploughing, and soil is broken to turn up soil carefully, plays furrow by north-south and waits to plant;In after the beginning of spring in the daytime temperature it is stable 15~ At 18 DEG C, carry out digging the non-irrigated lotus root tissue-cultured seedling of cave field planting away from 60~70cm with 70~80cm of line-spacing, row in the furrow ditch held successfully;
S4, field management:Seedling is looked into the progress of non-irrigated lotus root tissue-cultured seedling and filled a vacancy within 10~15 days after field planting;After field planting 20~30 days to Miao Zhugen Portion carries out first time ridging;Second is carried out to seedling strain root and is earthed up within 30~40 days after earthing up for the first time;Non-irrigated lotus root Post flowering is immediately Flower shears is gone;
S5, water and fertilizer management:200~250g of liquid manure is applied at away from non-irrigated 3~5cm of lotus root root when earthing up for the first time, during second of ridging 550~600g of liquid manure is applied at away from non-irrigated 8~10cm of lotus root root;The liquid manure includes the raw material of following parts by weight:Urea 15~ 25 parts, 10~15 parts of carbon ammonium, 10~12 parts of potassium chloride, 8~10 parts of ammonium phosphate, 1~3 part of potassium nitrate, 1~3 part of calcium nitrate, sulfuric acid 0.2~0.4 part of magnesium, 0.1~0.3 part of zinc chloride, 0.2~0.4 part of sodium molybdate, 0.1~0.3 part of ammonium molybdate, boric acid 0.1~0.3 Part, 0.1~0.3 part of salicylic acid, bitter 2~3 parts of lettuce, 2~3 parts of the root bark of shaggy-fruited dittany, 1~2 part of spinach spinach grass, 1~2 part of rheum officinale, the fruit of Cherokee rose 1 ~2 parts, 0.5~0.8 part of ginkgo leaf, 0.8~1.0 part of selfheal, 0.5~0.6 part of Sculellaria barbata, 40~50 parts of wine, arabinose 4~6 parts of alcohol, 2~4 parts of ribitol, 1~3 part of dulcitol, 1~3 part of glycerine, fatty acid methyl ester ethoxylate sulfonate 0.01~0.03 part and appropriate amount of water;
Wherein, the preparation method of the liquid manure comprises the following steps:
A. according to above-mentioned parts by weight, arabite, ribitol, dulcitol, glycerine is dissolved in the water of 100 parts by weight and obtained Sugar alcohol solution, then by magnesium sulfate, zinc chloride, sodium molybdate, ammonium molybdate, boric acid and fatty acid methyl ester ethoxylate sulfonate Sugar alcohol solution is added, 5~6h is stirred at 90~100 DEG C, sugar alcohol chelating Liquid Fertilizer is obtained;
B. according to above-mentioned parts by weight, by bitter lettuce, the root bark of shaggy-fruited dittany, spinach spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, half Lotus and wine are firstly placed in enamel pot, be then placed on it is another bigger be filled with water in pot, pot reclaimed water temperature control system that is filled with water is at 40~50 DEG C, so After bonnet enameling cover, 30~36h of water bath with thermostatic control, filter and remove residue obtains Chinese medicine liquid, and 50~60 weight are added in Chinese medicine liquid The water of number, obtains preventing disease and pest decoction;
C. according to above-mentioned parts by weight, urea, carbon ammonium, potassium chloride, ammonium phosphate is added and stirred in the water of 400~500 parts by weight Mix uniform, obtain composite fertilizer's feed liquid;
D. sugar alcohol made from step a is chelated into Liquid Fertilizer, composite fertilizer made from preventing disease and pest decoction made from step b and step c Feed liquid is mixed, after being sufficiently stirred for, that is, the liquid manure is made;
S6, the prevention and control of plant diseases, pest control:Non-irrigated lotus root growth period, occur bud rot, stem rot with 75% carbendazol wettable powder 800~ 1000 times of liquid are sprayed;There is virosis to be sprayed with 50% moroxydine wettable powder, 800~1000 times of liquid;There is rust and use 15% Clever 500~800 times of liquid of wettable powder of powder rust are sprayed;There is budworm to be sprayed with 50% dimethoate emulsifiable concentrate, 800~900 times of liquid; There is any of several broadleaf plants insect parasite which hurts rice plant to be sprayed with DDVP, 2000~2200 times of liquid of Amitraz compounded cream preparation.
2. the implantation methods as claimed in claim 1 for improving non-irrigated lotus root yield, it is characterised in that the depth that three times are turned in S3 is equal For 30~40cm.
3. the as claimed in claim 1 implantation methods for improving non-irrigated lotus root yield, it is characterised in that in S3 organic fertilizer be dung polluted water and Bean dregs, tartary buckwheat shell, chalina, cottonseed cake, orange peel, water hyacinth and water after crushing are according to mass ratio 15:1.4:0.8:1.7: 1.2:2.6:1.3:Become thoroughly decomposed and form after 5 mixing.
4. the implantation methods as claimed in claim 1 for improving non-irrigated lotus root yield, it is characterised in that every 5~6 days after being emerged in S4 A water is poured, soil moisture is kept between 60%~70%.
5. the as claimed in claim 1 implantation methods for improving non-irrigated lotus root yield, it is characterised in that when being earthed up for the first time in S4 also in Seedling mass fraction of strain foliage-spray is 3% potassium dihydrogen phosphate and the mixed solution of 2% ammonium hydrogen carbonate.
6. the implantation methods as claimed in claim 1 for improving non-irrigated lotus root yield, it is characterised in that bitter lettuce, the root bark of shaggy-fruited dittany, spinach in S5 Spinach grass, rheum officinale, the fruit of Cherokee rose, ginkgo leaf, selfheal, Sculellaria barbata are Fresh Plants.
7. the implantation methods as claimed in claim 1 for improving non-irrigated lotus root yield, it is characterised in that DDVP, Amitraz are multiple in S6 Include the raw material and auxiliary material of following parts by weight with cream preparation:25~30 parts of DDVP active compound, 10~12 parts of Amitraz active compound, 8~10 parts of emulsifying agent OPEO, 90 parts of solvent acetone.
CN201710299016.0A 2017-04-27 2017-04-27 Improve the implantation methods of non-irrigated lotus root yield Pending CN107155557A (en)

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