CN107144658B - A kind of Novel chromatographic column being vented - Google Patents
A kind of Novel chromatographic column being vented Download PDFInfo
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- CN107144658B CN107144658B CN201710511081.5A CN201710511081A CN107144658B CN 107144658 B CN107144658 B CN 107144658B CN 201710511081 A CN201710511081 A CN 201710511081A CN 107144658 B CN107144658 B CN 107144658B
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- column casing
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- column
- sealing ring
- upper flange
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/606—Construction of the column body with fluid access or exit ports
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The present invention provides a kind of Novel chromatographic column that can be vented, it is made of piston support component, piston, column casing upper flange, column casing, column casing lower flange, end-cap assembly, exhaust pipe, sealing ring A, sealing ring B and air discharge duct, it is set on piston or column casing upper flange there are two gas vent, gas vent is connected with exhaust pipe.The exhaust structure of chromatographic column can easily discharge air in column casing in the present invention, can not only improve the effective height of column casing, reduce chromatographic column total height, less have liquid and overflow and pollute piston.
Description
Technical field
The present invention relates to the Novel chromatographic columns that one kind can be vented, and belong to and prepare chromatographic field.
Background technique
Preparative scale chromatography column is the important technical that required target substance is isolated from complex fluid system, separation
When, it carries the mobile phase of sample and is moved in column bed by pressure-driven by the particle media of stationary phase, according to passing through medium
Mobility difference, the time that sample flow goes out chromatographic column is not also identical, is achieved in the separation of sample.The column of particle media
Bed forms under gravity, can be rinsed to be formed or be formed by the piston compression of chromatographic column with mobile phase, but residual in column bed
Air can generate air pocket between sample and the stationary phase of particle media, to directly affect separating effect.So making
Before chromatography post separation sample, it is necessary to remove remaining air from column bed gap, while to guarantee the close of entire chromatographic column
Feng Xing prevents extraneous gas from entering in chromatographic column.
Currently, being used to prepare type chromatographic column exhaust structure mainly has 2 kinds: the first structure will be vented as shown in Fig. 1
Slot is located at the middle position of column casing, and when piston reaches the position of air discharge duct, sealing ring A is in sealing state, at sealing ring B
In non-tight state, after being pumped into particle media into column casing, air is discharged outside column by sealing ring B by gas vent.The knot
Structure air discharge duct is placed among column casing, and column casing effective height reduces, and when loading the column bed of same height, chromatographic column overall height is wanted
It is higher by very much, virtually increases production cost, and not easy to operate.As shown in Fig. 2, the top of column casing is equipped with second of structure
Step, piston extend to the position of step, and sealing ring A and sealing ring B be all in non-tight state at this time, are pumped into column
After granular media, air is discharged outside column by sealing ring B and sealing ring A, since sealing ring A and sealing ring B locate
When non-tight state, exhaust, gas is easy to clip liquid to be discharged together, and spills over the upper surface of piston.Chromatography in the present invention
The exhaust structure of column can easily discharge air in column casing, can not only improve the effective height of column casing, and it is total to reduce chromatographic column
Highly, liquid is less had to overflow and pollute piston.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of Novel chromatographic column that can be vented, specific technical solution is as follows:
A kind of Novel chromatographic column being vented, by piston support component, piston, column casing upper flange, column casing, column casing lower flange, end cap group
Part, exhaust pipe, sealing ring A, sealing ring B and air discharge duct composition；Column casing top is connect with column casing upper flange, is set between the two
There is an air discharge duct, column casing lower part is connect with column casing lower flange, and column casing lower flange bottom is equipped with end-cap assembly, and piston is mounted on work
Support component lower part is filled in, is moved up and down along column casing axis, sealing ring A and sealing ring B, column casing inner wall, column are housed on the outside of piston
Cylinder upper flange forms a confined space between piston and sealing ring A, set that there are two exhausts on piston or column casing upper flange
Hole, gas vent are connected with exhaust pipe.
Present invention has the advantage that (1) present invention is to start to be vented after piston enters column casing, piston, column casing at this time
A confined space is formd with end cap, air cannot be introduced into column casing；(2) exhaust position of the present invention is located at column casing inner wall and work
Between plug, facilitates and exclude the air in column casing inner wall, piston and the formed space sealing ring B completely；(3) column casing of the present invention
When interior gas is discharged outside column casing by hose, will not entrained liquids to overflow column outer and pollute piston；(4) at air discharge duct of the invention
Between column casing and column casing upper flange, structure is simple, facilitates processing；(5) column casing upper flange participates in sealing, can reduce the height of column casing
Degree, to reduce the whole height of chromatographic column.
Detailed description of the invention
Fig. 1 is the first exhaust structure enlarged diagram of the prior art
Fig. 2 is second of exhaust structure enlarged diagram of the prior art
Fig. 3 is the first embodiment exhaust position structural schematic diagram of the invention
Fig. 4 is the enlarged diagram in Fig. 3 at A
Fig. 5 is the first embodiment normal operating conditions schematic diagram of the invention
Fig. 6 is second of embodiment exhaust position structural schematic diagram of the invention
Fig. 7 is the enlarged diagram in Fig. 6 at B
Fig. 8 is second of embodiment normal operating conditions schematic diagram of the invention
Specific code name is as follows in figure: 1. piston support component, 2. piston, 3. column casing upper flange, 4. column casing, 5. column casing laxative remedy
11. gas vent of blue 6. end-cap assembly, 7. exhaust pipe, 8. sealing ring A, 9. sealing ring B, 10. air discharge duct, 12. step.
Specific embodiment
In conjunction with Fig. 3 ~ Fig. 8, present invention is further described in detail.
The first embodiment of the invention is shown in Fig. 3 ~ Fig. 5, by piston support component 1, piston 2, column casing upper flange 3,
Column casing 4, column casing lower flange 5, end-cap assembly 6, exhaust pipe 7, sealing ring A 8, sealing ring B 9 and 10 groups of air discharge duct
At being set on piston 2 there are two gas vent 11, gas vent 11 is connected with exhaust pipe 7.
Piston 2 is moved to exhaust position (such as Fig. 3) by chromatographic column in exhaust, sealing ring A 8 and method on column casing
Blue 3 gluing, sealings, sealing ring B 9 are in the position of air discharge duct (10), form non-tight state, the gas vent on piston 2
11 and exhaust pipe 7 communicate with the outside world.Particle media is pumped in column casing 4 from end-cap assembly 6, and particle media is continuous
Rise, air is rushed to, 10 position of air discharge duct is formed by by 4 top of column casing and 3 lower part of column casing upper flange, and passed through
Air discharge duct 10 is discharged to outside column casing (such as Fig. 4) through exhaust pipe 7, and after exhaust, piston 2 is moved downward, at chromatographic column
In normal operating conditions, sealing ring A 8, sealing ring B 9 and the fitting of 4 inner wall of column casing are prevented at sealing state (such as Fig. 5)
Extraneous gas enters.
Second of embodiment of the invention is shown in Fig. 6 ~ Fig. 8, by piston support component 1, piston 2, column casing upper flange 3,
Column casing 4, column casing lower flange 5, end-cap assembly 6, exhaust pipe 7, sealing ring A 8, sealing ring B 9 and 10 groups of air discharge duct
At being set on column casing upper flange 3 there are two gas vent 11, gas vent 11 is connected with exhaust pipe 7.
Piston 2 is moved to exhaust position (such as Fig. 6) by chromatographic column in exhaust, sealing ring A 8 and method on column casing
Blue 3 gluing, sealings, sealing ring B 9 be in the position of air discharge duct (10), formation non-tight state, in column casing upper flange 3
Gas vent 11 and exhaust pipe 7 communicate with the outside world.Particle media is pumped in column casing 4 from end-cap assembly 6, graininess
Medium constantly rises, and air is rushed to and is formed by air discharge duct 10 by 4 top of column casing and 3 lower part of column casing upper flange
It sets, and is discharged to outside column casing (such as Fig. 7) by air discharge duct 10 through exhaust pipe 7, after exhaust, piston 2 is transported downwards
Dynamic, chromatographic column is in normal operating conditions, and sealing ring A 8, sealing ring B 9 and the fitting of 4 inner wall of column casing are at sealing state
(such as Fig. 8) prevents extraneous gas from entering.
Claims (1)
1. the Novel chromatographic column that one kind can be vented, by piston support component (1), piston (2), column casing upper flange (3), column casing (4),
Column casing lower flange (5), end-cap assembly (6), exhaust pipe (7), sealing ring A(8), sealing ring B(9) and air discharge duct (10) composition；
Column casing (4) top is connect with column casing upper flange (3), is equipped with an air discharge duct (10) between the two, under column casing (4) lower part and column casing
Flange (5) connection, column casing lower flange (5) bottom are equipped with end-cap assembly (6), and piston (2) is mounted under piston support component (1)
Portion, along column casing (4), axis is moved up and down, and sealing ring A(8 is housed on the outside of piston) and sealing ring B(9), column casing (4) inner wall, column
Cylinder upper flange (3), piston (2) and sealing ring A(8) between one confined space of formation, it is characterised in that: piston (2) or column casing
It is set on upper flange (3) there are two gas vent (11), gas vent (11) is connected with exhaust pipe (7)；Chromatographic column is in exhaust, by piston
(2) exhaust position, sealing ring A (8) and column casing upper flange (3) gluing, sealing, sealing ring B(9 are moved to) it is in air discharge duct
(10) position forms non-tight state, and the gas vent (11) and exhaust pipe (7) on piston (2) communicate with the outside world.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710511081.5A CN107144658B (en) | 2017-06-29 | 2017-06-29 | A kind of Novel chromatographic column being vented |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710511081.5A CN107144658B (en) | 2017-06-29 | 2017-06-29 | A kind of Novel chromatographic column being vented |
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| Publication Number | Publication Date |
|---|---|
| CN107144658A CN107144658A (en) | 2017-09-08 |
| CN107144658B true CN107144658B (en) | 2019-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710511081.5A Active CN107144658B (en) | 2017-06-29 | 2017-06-29 | A kind of Novel chromatographic column being vented |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4470910A (en) * | 1982-02-25 | 1984-09-11 | Instruments S. A. | Chromatographic apparatus and process for its use |
| CN2906618Y (en) * | 2006-05-02 | 2007-05-30 | 江苏汉邦科技有限公司 | Dynamic axial compression chromatographic column |
| CN202393748U (en) * | 2011-10-14 | 2012-08-22 | 齐齐哈尔大学 | Internal gas removing device of liquid phase chromatograph |
| CN202478652U (en) * | 2012-03-22 | 2012-10-10 | 利穗科技(苏州)有限公司 | Chromatographic column |
| CN203227330U (en) * | 2013-05-24 | 2013-10-09 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrially preparative chromatographic column |
| CN204745742U (en) * | 2015-06-18 | 2015-11-11 | 江苏汉邦科技有限公司 | No pole developments axial compression post |
| CN206848222U (en) * | 2017-06-29 | 2018-01-05 | 江苏汉邦科技有限公司 | A kind of Novel chromatographic column being vented |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8128816B2 (en) * | 2009-06-19 | 2012-03-06 | Bio-Rad Laboratories, Inc. | Piston movement control for preparative chromatography column |
-
2017
- 2017-06-29 CN CN201710511081.5A patent/CN107144658B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4470910A (en) * | 1982-02-25 | 1984-09-11 | Instruments S. A. | Chromatographic apparatus and process for its use |
| CN2906618Y (en) * | 2006-05-02 | 2007-05-30 | 江苏汉邦科技有限公司 | Dynamic axial compression chromatographic column |
| CN202393748U (en) * | 2011-10-14 | 2012-08-22 | 齐齐哈尔大学 | Internal gas removing device of liquid phase chromatograph |
| CN202478652U (en) * | 2012-03-22 | 2012-10-10 | 利穗科技(苏州)有限公司 | Chromatographic column |
| CN203227330U (en) * | 2013-05-24 | 2013-10-09 | 江苏迪沃特仪器设备科技有限公司 | Guard column of industrially preparative chromatographic column |
| CN204745742U (en) * | 2015-06-18 | 2015-11-11 | 江苏汉邦科技有限公司 | No pole developments axial compression post |
| CN206848222U (en) * | 2017-06-29 | 2018-01-05 | 江苏汉邦科技有限公司 | A kind of Novel chromatographic column being vented |
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| Publication number | Publication date |
|---|---|
| CN107144658A (en) | 2017-09-08 |
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Address after: 223005 no.1-9 Jixian Road, Huaian Economic and Technological Development Zone, Jiangsu Province Patentee after: Jiangsu Hanbang Technology Co.,Ltd. Address before: 223005 no.1-9 Jixian Road, Huaian Economic and Technological Development Zone, Jiangsu Province Patentee before: JIANGSU HANBON SCIENCE & TECHNOLOGY Co.,Ltd. |
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Denomination of invention: A new type of gas chromatographic column Effective date of registration: 20221206 Granted publication date: 20191015 Pledgee: Industrial Bank Co.,Ltd. Huai'an branch Pledgor: Jiangsu Hanbang Technology Co.,Ltd. Registration number: Y2022980025314 |