CN107137443A - A kind of composition for relieving fatigue, preparation method and applications - Google Patents
A kind of composition for relieving fatigue, preparation method and applications Download PDFInfo
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- CN107137443A CN107137443A CN201710281445.5A CN201710281445A CN107137443A CN 107137443 A CN107137443 A CN 107137443A CN 201710281445 A CN201710281445 A CN 201710281445A CN 107137443 A CN107137443 A CN 107137443A
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- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
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Abstract
The present invention provides a kind of composition for relieving fatigue, the composition is made up of maca microcapsule powder, American ginseng extract and pharmaceutically acceptable auxiliary material, wherein described maca microcapsule powder is by low-temperature airflow crushing broken wall, inclusion, solid disperses, microencapsulation is made, and the American ginseng extract is made using stem and leaves of American ginseng and stem tuber as raw material is extracted.In the present invention, low-temperature airflow broken wall crushing technology ensures that maca volatile oil exposes and is retained in ultrafine powder to greatest extent, is conducive to the absorption and utilization of active ingredient.Compared with maca raw material, maca superfine powder, common maca preparation, the 24 months acceleration for stabilization rates and absorptivity of maca volatile oil are significantly improved, and effective time substantially shortens.Present invention incorporates maca " tonifying the kidney and support yang " and the action character of American Ginseng " boosting qi and nourishing yin ", meet " treating YIN within YANG; treating YANG within YIN ", " coordination of YIN and YANG ", the theory of traditional Chinese medical science of " equilibrium between yin and yang ", the people for being adapted to the deficiency of Yin, the deficiency of yang or yin-yang deficiency constitution eats, and plays fatigue-relieving, improves the effect of physical efficiency.
Description
Technical field
The present invention relates to health care technology field, more particularly to a kind of fatigue-relieving prepared by maca and American Ginseng
Composition, the preparation method and application of said composition.
Background technology
Fatigue is a kind of normal physiological phenomenon, is a kind of protection mechanism for people.When we feel fatigue, explanation
Body sends the signal that rest to us.No matter if ignored it, body will suffer damage, and finally fall sick from overwork.
The life of daily working from 9am to 5pm, the physical exertion based on muscle activity, or the mental work based on spirit and thinking activities
It is dynamic, by the regular hour and reach that certain degree can all cause the decline of mobility, show as tired or DOMS
Or general weakness, i.e., usual described fatigue.Today's society, with the progress and expanding economy of science and technology, the life pressure of people
Power is increasing, and fatiguability crowd is also more and more, and fatigue is the main forms of human-body sub-health, is Recent study
One of focus, alleviating physical fatigue turns into people's increasing need.
With the economic development and improvement of people's living standards, the consumption idea of people, concept of health there occurs larger
Change, the consumption demand to health food is increasingly improved, in addition, living-pattern preservation, multi-level social life needs,
Wide development space is provided for Health-care Foods Industry.Accordingly, it would be desirable to a kind of effective health food for relieving fatigue is developed, it is full
The sufficient people demand growing to health.
Maca volatile oil is the important component that maca product plays effect, and maca product volatile oil in the market contains
Amount is few, and stability and bioavilability be not high, and anti-fatigue effect effective time is long, does not reach the effect of timely fatigue-relieving.
In American Ginseng product, saponin(e and polysaccharide isoreactivity composition are extracted using stem tuber, and recovery rate is only 10%, total saponin content 1~
Between 5%.Other positions of American ginseng medicine can not make full use of, and cause the waste of resource.Therefore, special process and ratio are passed through
Example prepares maca American ginseng has great economy and social effect to significantly improve antifatigue effect.
The content of the invention
It is therefore an object of the present invention to provide a kind of more effective composition for relieving fatigue, the composition is micro- by maca
Capsule powder, American ginseng extract and optional pharmaceutically acceptable auxiliary material composition.
Preferably, the maca microcapsule powder in composition of the present invention is by the crushing of low-temperature airflow broken wall, inclusion, solid
Scattered, encapsulation process is made, and American ginseng extract is using obtained by stem and leaves of American ginseng and stem tuber are raw material extraction.
In composition of the present invention, the weight of maca microcapsule powder and American ginseng extract than preferably 350: 15~
30, more preferably 350: 18~25, more preferably 350: 20.
In composition of the present invention, the content of volatile ingredient is preferably to press maca microcapsule powder in maca microcapsule powder
Weight meter 0.5~2.0%, more preferably 0.8~1.7%, more preferably 1.0~1.5%.
In composition of the present invention, the content of total saposins is preferably to press American ginseng extract in American ginseng extract
Weight meter 15~25%, more preferably 17~23%, more preferably 18~21%.
Pharmaceutically acceptable auxiliary material can be added in composition of the present invention, capsule, tablet, particle is prepared into
The solid orally ingestibles such as agent, micropill preparation.Preferably, in the solid orally ingestible, maca microcapsule powder and American ginseng extract
Gross mass and auxiliary material quality be 22: 1.It is highly preferred that the auxiliary material is selected from solid dispersible carrier, starch, magnesium stearate
Or the one or more in microcrystalline cellulose.
According to composition of the present invention embodiment, the composition is by by maca microcapsule powder, the West
Conopsea extraction, the mixing of solid dispersible carrier, are prepared through solid dispersing technology；Preferably, the solid dispersible carrier is selected from
One or more in PVP K30, PEG-4000, poloxamer-188, xylitol or sorbierite.
Present invention also offers a kind of preparation method of composition of the present invention, wherein the preparation method is including following
Step：
(1) maca is subjected to low-temperature airflow broken wall crushing, then included obtained maca fine powder successively, solid divides
Dissipate, microencapsulation is handled, and maca microcapsule powder is made；
(2) cauline leaf and stem tuber of American Ginseng are taken, use concentration of volume percent for 70~80% ethanol water hot dipping
Extract, leaching liquor is concentrated, adsorbed with macroporous absorbent resin, then successively using 70~80% ethanol water, water elution,
Eluent is merged, concentrated, is dried, American ginseng extract is obtained；
(3) maca microcapsule powder, American ginseng extract, optional pharmaceutically acceptable auxiliary material are mixed, mixing is produced.
Preferably, the step of the inventive method in (1), the low-temperature airflow broken wall is crushed in N2, at a temperature of -20 DEG C
Carry out, grinding particle size is 800~1200 mesh.
Preferably, the step of the inventive method in (1), the inclusion is：Space net structure inclusion material is weighed, plus
Enter 2~5 times of the water based on inclusion quality of materials, grinding is uniform, then adds maca fine powder, grinds to form after pasty state at 2~10 DEG C
At a temperature of dry, obtain include product.It is highly preferred that the inclusion material is selected from beta-schardinger dextrin, alpha-cyclodextrin, γ-ring paste
One or more in essence or gas phase superfine silica gel powder.
Preferably, the step of the inventive method in (1), the microencapsulation is carried out using phase separation-coacervation；More preferably
One kind in sodium alginate, sodium carboxymethylcellulose, PEG or Arabic gum of the coating material used in ground, microencapsulation or
It is a variety of.
Preferably, the step of the inventive method in (3), its operation is：By maca microcapsule powder, American ginseng extract, consolidate
Body dispersible carrier is mixed, and adds appropriate based on concentration of volume percent 70~75% ethanol water, and strong stirring 4~5 is small
When, it is uniformly dispersed, obtain solid dispersions；Wherein, the quality of solid dispersible carrier is that maca microcapsule powder and American Ginseng are carried
Take the 0.5~5% of thing gross mass.
The step of preparation in accordance with the present invention, wherein methods described also include composition preparation is made.
The present invention also provides composition of the present invention in fatigue-relieving medicine, health food, feature ordinary food, spy
Application in different medical food or special diet food.
Present invention employs " crushing of low-temperature airflow broken wall " technology, it is ensured that maca volatile oil exposes and retained to greatest extent
In ultrafine powder, be conducive to the absorption and application of active ingredient.Maca microcapsule powder, energy are prepared using inclusion and microencapsulation
Enough significantly improve maca stability of volatile oil and bioavilability.Maca microcapsule powder of the present invention and maca raw material, maca
Superfine powder, common maca preparation are compared, and 24 months acceleration for stabilization rates of maca volatile oil improve 20~35 times, and absorptivity improves 4
More than times, effective time (influence to mouse sexual behaviour) shortens 1/3.
In the preparation technology of the present invention, following effect can be reached using solid dispersion technology：(1) ensure to be sufficiently mixed
Uniformly, accurate measurement；(2) maca, the stability of American Ginseng functional component are further improved；(3) maca, the West are further improved
Join the bioavilability of functional component；(4) bad smell of maca is further covered；(5) it is easy to follow-up formulation to prepare.
In the present invention, American ginseng extract uses stem and leaves of American ginseng, stem tuber for raw material, with " total saposins ", " polysaccharide " etc.
Bioactive ingredients are Objective extraction object, are handled respectively using hot dipping, macroporous absorbent resin separation, adsorbate and effluent
Mode carries out full composition extracting, extracts yield and is promoted to more than 20%, far above the extraction yield of existing method 10% or so.This
Outside, method of the invention has been sufficiently reserved the bioactive ingredients such as Radix Panacis Quinquefolii polysaccharide, total saposins, and total saponin content is promoted to 15~
30%, the total saponin content far above in existing method 1~5% realizes carrying for stem and leaves of American ginseng, stem tuber and its full composition
Take, study, develop, apply, it is ensured that nutrition, effective performance of healthcare function.
Compared with prior art, the present invention has following technical advantage：A, tradition, which are extracted, uses American Ginseng stem tuber, this project
Use stem and leaves of American ginseng, stem tuber for raw material, economize on resources；B, prior art are extracted mainly for " total saposins " for target compound,
The present invention is used as extracting object for bioactive ingredients such as " total saposins ", " polysaccharide "；C, prior art use " water boiling and precipitation with ethanol ",
Extract of the present invention is obtained through hot dipping, macroporous absorbent resin separation, full nutrition and functional component collection, low temperature drying.It is logical
Above-mentioned processing is crossed, following Substantial technical effect is obtained：(1) stem and leaves of American ginseng is made full use of, medicine resource is saved；(2) extract
Thing yield is high, more than 20%；(3) full nutrition and functional component extracting (Radix Panacis Quinquefolii polysaccharide, total saposins)；(4) total saponin content can
Up to 15~30%.
The present invention composition using predetermined substance, special ratios carry out prescription, fully apply maca " kidney tonifying is helped
Sun " and " boosting qi and nourishing yin " perfect adaptation of American Ginseng, meet theory of traditional Chinese medical science " treating YIN within YANG, treating YANG within YIN ", " coordination of YIN and YANG ",
" equilibrium between yin and yang " arranges in pairs or groups.Both the effect of maca tonifying kidney and strengthening yang had been played, the boosting qi and nourishing yin function of American Ginseng, yin-deficiency constitution are combined again
People it is edible will not also damage body, the people for complying fully with the deficiency of Yin, the deficiency of yang or yin-yang deficiency constitution eats, more, preferably make
Good fortune needs edible maca, American Ginseng but the different subject of constitution.
Embodiment
Embodiment 1
The preparation of maca microcapsule powder
A. crush：Select in the maca medicinal material of high-quality, feeding cryogenic gas pulverizer, pass through stream of nitrogen gas powder at -20 DEG C
Broken, powder crosses 800 eye mesh screens, obtains maca fine powder.
B. include：The beta-schardinger dextrin of recipe quantity is taken, water grinding is added uniformly, then adds maca fine powder made from step A,
Grind to form and dried at 5 DEG C after pasty state, obtain including product.
C. solid disperses：0.5~5% gas added in the inclusion product obtained to step B based on inclusion products weight
Phase superfine silica gel powder, strongly grinds 4~5 hours after mixing, it is uniformly dispersed, and forms solid dispersions.
D. microencapsulation：Solid dispersions made from step C are placed in fluid bed, taken 0.5 based on inclusion products weight ratio
~2% sodium carboxymethylcellulose, after being dissolved with water, is added in fluid bed, boiling makes its abundant microencapsulation, so as to obtain described
Maca microcapsule powder.
Embodiment 2
The effect experiment of maca microcapsule powder
(1) stability test
1st, sample preparation：1. number the maca microcapsule powder obtained from embodiment 1 takes sample, fills as 100g/ bags, is designated as；Take general
2. number logical pueraria root powder, is filled as 100g/ bags, is designated as.
2nd, test specimen is placed 6 months under conditions of 40 DEG C of temperature, relative humidity 75%, carries out accelerated stability examination
Test (2010 editions《Chinese Pharmacopoeia》Two, P annex 200), sample, survey respectively at 0 month, 1 month, 2 months, 3 months, 6 months
Determine volatile oil content.
3rd, volatile oil content testing method：Sample 1. and 2. each 10g is taken, 100mL water is added, mixing is followed by volatile oil and carried
Device and reflux condensing tube are taken, according to volatile oil extracting standard method (2010 editions《Chinese Pharmacopoeia》One, P annex 63) carried
Take, extraction time is 8h, extract the distillate containing volatile oil using 45 DEG C of absolute ethers steamed again, ether extraction liquid passes through anhydrous
After sodium sulphate is dried, simultaneously constant volume, to 20mL, is detected 45 DEG C of recovery absolute ethers with gas chromatograph-mass spectrometer.
Detection method：0.1mol/L benzene acetonitrile standard liquid is prepared, gas chromatography mass spectrometry detection is carried out, wherein vapor detection is joined
Number is as follows：HP-5MS fused-silica capillary columns (30m × 0.25mm, 0.25 μm), heating schedule is 35 DEG C of maintenance 2min, with
15C/min rises to 215 DEG C, keeps 16min；Carrier gas (He) flow velocity 1.0mL/min, pressure 2.4kPa, the μ L of sample size 1.0；Shunting
Than 50: 1.
Mass Spectrometer Method condition is as follows：MSD Chemstation E.01.00.237 work station, electron bombardment (EI) ion
Source；230 DEG C of ion source temperature, ionization voltage 70eV；275 DEG C of transmission line temperature；50~500m/z of mass scan range, scanning speed
Rate 2scans/s；Parent ion 285m/z；Activate voltage 1.5V.
Result of the test is shown in Table 1.
Table 1
Result of the test shows：Maca microcapsule powder (1. number sample) of the present invention volatile oil content in 12 months is stable,
Change not clear aobvious at 24 months, only exhaustion 2.26%；Common pueraria root powder (2. number sample) volatile oil content is relatively steady in 3 months
It is fixed, but after 3 months, content is substantially reduced, and to during off-test, content is only 0.04%, and exhaustion rate is 97.67%
(2) absorptivity is tested
1st, sample preparation：1. and 2. number the gained sample of Example 1 and common pueraria root powder, are denoted as；
2nd, test method：Using body weight about 3kg healthy rabbits, (by Sichuan University, Experimental Animal Center is carried experimental animal
For), animal is randomly divided into after four groups, advance fasting 12h, and gavage gives above-mentioned sample respectively, dosage be 200mg/kg (equivalent to
70kg Coming-of-Age Days taking dose 4g), give after sample it is intensive measure blood plasma in volatile oil content, to peak time half an hour after,
Detect blood peak concentration of drug (Cmax)；
After test specimen gavage terminates, be discontinued a period of time, be metabolized completely to animal vivo sample, be then injected intravenously to
The dosage volatile oil solution such as give, and determine volatile oil content in blood plasma (μ g/mL), in this, as reference data.
Absorptivity (%)=(vein is to volatile oil content in sample blood plasma/and orally give volatile oil content in sample blood plasma) ×
100%
It the results are shown in Table 2.
Table 2
| Test specimen | ① | ② |
| Dosage (g) | 0.6 | 0.6 |
| Volatile oil content (%) | 1.77 | 1.72 |
| Volatile oil dosage (mg) | 10.62 | 10.32 |
| Vein is to sample blood plasma volatilization oil concentration (μ g/mL) | 48.27 | 46.91 |
| Orally give volatile oil Cmax C in sample blood plasmamax | 44.92 | 11.14 |
| μg/mL | ||
| Absorptivity | 93.06 | 23.74 |
Result of the test shows：Volatilization oil absorption rate highest, is common in maca microcapsule powder (1. number sample) of the present invention
3.92 times of pueraria root powder (2. number sample).
(3) experiment influenceed on mouse sexual behaviour
1st, sample preparation：1. and 2. number the gained sample of Example 1 and common pueraria root powder, are denoted as.
2nd, test method：Experimental animal uses Kunming mouse, and cleaning grade, male and female have concurrently, and 18~22g of body weight is (by Sichuan
University's Experimental Animal Center is provided)；Male mice is randomly divided into control group and two test groups, every group 10.Two test groups
Gavage gives above-mentioned sample respectively, and dosage is 200mg/kg (equivalent to 70kg Coming-of-Age Days taking dose 4g), and control group gavage is given
The dosage physiological saline such as give, continuous gavage 120 days carried out tests below respectively at the 30th day, the 60th day, the 90th day, the 120th day
Observation：48h female sub-cutaneous injections oestradiol benzoates (0.02mg/ is only), makes estrus identical before experiment, test in evening 7~
Carried out when 11, male mice is placed individually into cage (55cm × 35cm × 20cm) 5min, environment is adapted it to, then by 1: 3
Ratio mates male mouse and female mice, observed and recorded mating incubation period (from female mice put into male mouse mated with female mice for the 1st time when
Between) and 1h in number of copulations.
It the results are shown in Table 3 and table 4.
Influence of the different tests sample of table 3 to male mice mating incubation period (s)
Note：Compared with control group, * P<0.05, * * P<0.01 (P values be statistically represent real result degree (being capable of generation
Table is overall) a kind of method of estimation.P<0.05 indicates significant difference, P<0.01 indicates notable significant difference.
Influence of the different tests sample of table 4 to male mice mating number of times (in lh)
Note：Compared with control group, * P<0.05, * * P<0.01 (P values be statistically represent real result degree (being capable of generation
Table is overall) a kind of method of estimation.P<0.05 indicates significant difference, P<0.01 indicates notable significant difference.
Result of the test：
(1) mate incubation period
Maca microcapsule powder (1. number sample) of the present invention is in experiment the 30th, 60,90,120 day mating incubation period with compareing
Group is compared, and has notable significant difference；Common pueraria root powder (2. number sample) is only in the 90th, 120 day mating incubation period with compareing
There were significant differences for group ratio.To sum up, the mating onset time in incubation period (30 days) of maca microcapsule powder (1. number sample) is better than commonly
Pueraria root powder (2. number sample) (90 days).
(2) mating number of times
Maca microcapsule powder (1. number sample) of the present invention in experiment the 30th, 60,90,120 days 1h mating number of times with it is right
Compared according to group, there is significant difference；Common pueraria root powder (2. number sample) is only the 90th, in 120 days 1h mating number of times with compareing
There were significant differences for group ratio.To sum up, in the 1h of maca microcapsule powder (1. number sample) mating number of times onset time (30 days) be better than it is general
Logical pueraria root powder (2. number sample) (90 days).
This result of the test shows：The effective time of maca microcapsule powder (1. number sample) of the present invention compares common pueraria root powder
(2. number sample) shortens 1/3.
Above correlation test result shows that the stability and absorptivity of maca microcapsule powder of the present invention are significantly larger than commonly
Pueraria root powder, onset time greatly shortens.
Embodiment 3
The preparation of American ginseng extract
Clean stem and leaves of American ginseng and stem block is taken, is that 1: 10 addition concentration of volume percent is by mass volume ratio (kg/L)
70~80% ethanol water, is extracted 2.5~3 hours at a temperature of 75 DEG C~80 DEG C, continuous to extract 3 times.It will extract
To extract solution be concentrated under reduced pressure into the 1/10 of original volume, upper macroporous absorbent resin is first 70~80% with concentration of volume percent
Ethanol water elution, be washed with water de-, collect eluent, alcohol is eluted and the obtained two parts eluent of water elution is closed
And, concentrate, dry, obtain American ginseng extract.
Embodiment 4
It is prepared by maca American Ginseng solid dispersions
A. dispensing：Maca microcapsule powder and American ginseng extract are weighed in 17.5: 1 ratio；By maca microcapsule powder and the West
The 0.5-5% of conopsea extraction gross weight weighs solid dispersible carrier PEG-4000.
B. mix：Maca microcapsule powder, American ginseng extract and solid dispersible carrier are well mixed.
C. solid disperses：In the mixture obtained to B, appropriate based on concentration of volume percent 70~75% ethanol is added
The aqueous solution, strong stirring 4~5 hours, makes it be uniformly dispersed, obtains solid dispersions.
Embodiment 5
The preparation of maca American ginseng capsule
According to the general production process and this product feature of capsule, the process route for determining this product is：Sieve, mix, always
Mixed, filling, polishing, packaging, inspection, storage.
Sieving：Each supplementary material crosses 80 mesh sieves, then is fed intake in the ratio weighing of formula, to ensure the addition of supplementary material,
And beneficial to mixing.
Mixing：Maca microcapsule powder, American ginseng extract, starch are mixed and mixed 30 minutes for preparation dispensing herein, obtains mixed
Close powder.Mixed powder uniform color, shows to be well mixed.Result of the test see the table below 5.
Table 5
| Incorporation time | Mixed powder color and luster |
| 10 minutes | Color and luster is uneven |
| 20 minutes | Color and luster is uneven |
| 30 minutes | Uniform color |
Magnesium stearate is mixed 10 minutes with mixed powder, is well mixed, obtains and always mixes material.Using angle of repose as index, examine
The mobility of mixed material is examined, as a result such as table 6 below.
Table 6
Enlarged experiment is carried out according to the production technology of laboratory research, process certification is carried out and inclined to the production technology drafted
Difference is corrected.
With reference to scale up test situation, pair formula determined, technique have carried out three batches of scale up test, and pilot scale data see the table below 7.
7 three batches of scale up test data summary tables of table
Three batches of pilot scales show that identified process stabilizing can be applied to production；Products obtained therefrom quality meets kind enterprise
The requirement of industry standard.
Embodiment 6
The antifatigue compliance test result of maca American ginseng capsule
Test method：
Test group 1 is set respectively：The maca American ginseng capsule of 20 times of human body recommended dose, human body recommended amounts are given daily
2.4g/60kg.d, i.e., give dosage 800mg/kg.bw daily.
Test group 2：Dosage 800mg/kg.bw maca American Ginseng solid dispersions are given daily.
Blank group：The distilled water of equivalent is given daily.
Control group 1：800mg/kg.bw maca microcapsule powder is given daily.
Control group 2：800mg/kg.bw American ginseng extract is given daily.
Above-mentioned five groups of animal mouse, carry out four experiments and amount to 200 by every group 10.Maca American Ginseng glue is weighed respectively
Capsule 20g, maca American Ginseng solid dispersions 20g, maca microcapsule powder 20g, American ginseng extract 20g, respectively add distilled water to 500ml
Mix, it is stored refrigerated, it is finished and matches somebody with somebody again.Oral gavage is distinguished to mouse by 20ml/kg.bw, is administered once per day for the treatment of, continuous 30 days.
Blank group directly gives 20ml/kg.bw distilled water.Then carry out following test：
(1) the mice burden swimming time tests
The mouse last run an experiment is given after tested material 30min, by the insurance of the body weight of rat-tail portion load 5%
Silk, then mouse is put into 25 ± 1 DEG C of water temperature, depth of water 30cm swimming trunk went swimming records mouse from swimming is started to death
Swimming time.Experimental result is as shown in table 8.By the visible maca American ginseng capsule of table 8, the heavy burden of maca American Ginseng solid dispersions
Swimming time is compared with blank group, is statistically analyzed, and there were significant differences for walking weight load extension, and maca American Ginseng glue
Capsule is compared with maca American Ginseng solid dispersions, to the extension mice burden swimming time without significant difference.With the maca west of dosage
American ginseng capsule is compared with the maca microcapsule powder of same dosage, and to the extension mice burden swimming time, also there were significant differences；With dosage
Maca American ginseng capsule is compared with the American ginseng extract of same dosage, and to the extension mice burden swimming time, also there were significant differences.
Influence of the maca American ginseng capsule of table 8 to mouse swimming time
(2) serum urea determination experiment after mouse movement
Laboratory mice last is given after tested material 30min, not swimming with a load attached to the body 90min in the water of 30 DEG C of water temperature is put into,
After rest 60min, eyeball blood sampling 0.5ml is pulled out, the content of serum urea, experimental result such as table 9 is determined.From table 9, it will test
Serum urea and blank group after group 1, the mouse movement of test group 2 are compared, and are statistically analyzed, and serum urea reduction has
Significant difference.And control group 1, control group 2 are compared the reduction of serum urea with blank group respectively that there was no significant difference.
Influence of the maca American ginseng capsule of table 9 to serum urea after mouse movement
(3) experiment of mouse hepatic glycogen
Laboratory mice last is given after tested material 30min, puts to death animal and takes liver to use filter after being rinsed through physiological saline
Paper is blotted, and is weighed 100mg livers and is determined hepatic glycogen content.By test group 1, the hepatic glycogen content of the mouse of test group 2 and blank group
It is compared, is statistically analyzed, hepatic glycogen content is significantly higher than blank group.And control group 1, control group 2 respectively with blank group
Compare, hepatic glycogen content is without significant difference.Experimental result is as shown in table 10 below.
Influence of the maca American ginseng capsule of table 10 to mouse hepatic glycogen
(4) after mouse movement blood lactase acid level determination experiment
Laboratory mice last is given after tested material 30min, is put into the water of 30 DEG C of water temperature after not swimming with a load attached to the body 10min
Stop.Determine swimming before, after swimming and swimming after rest 20min blood lactase acids content.As a result such as table 11.From table 11, examination
Test group 1, test group 2 and control group 1, the blood lactase acid TG-AUC of control group 2 respectively with blank group to be compared, at statistics
Reason, blood lactase acid TG-AUC there are no significant difference.
Participate in the influence that capsule changes to blood lactase acid after mouse movement by shares in the maca of table 11 the West
Four experimental datas more than, maca American ginseng capsule can effective fatigue-relieving, effect is substantially better than list
The maca microcapsule powder of side.Maca American ginseng capsule is compared with maca American Ginseng solid dispersions, and fatigue-relieving effect is substantially the same.
Claims (10)
1. a kind of composition for relieving fatigue, the composition is by maca microcapsule powder, American ginseng extract and optional pharmacy
Upper acceptable auxiliary material composition；Preferably, the maca microcapsule powder is scattered, micro- by low-temperature airflow crushing broken wall, inclusion, solid
Encapsulated is made, and the American ginseng extract is made using stem and leaves of American ginseng and stem tuber as raw material is extracted.
2. composition according to claim 1, it is characterised in that the weight of the maca microcapsule powder and American ginseng extract
Than for 350: 15~30, preferably 350: 18~25, more preferably 350: 20.
3. composition according to claim 1, it is characterised in that the content of maca volatile oil is in the maca microcapsule powder
0.5~2.0% based on maca microcapsule powder weight, preferably 0.8~1.7%, more preferably 1.0~1.5%.
4. composition according to claim 1, it is characterised in that in the American ginseng extract content of total saposins be by
American ginseng extract weight meter 15~25%, preferably 17~23%, more preferably 18~21%.
5. composition according to any one of claim 1 to 4, it is characterised in that the composition includes pharmaceutically may be used
The auxiliary material of receiving, wherein in the composition, the gross mass of maca microcapsule powder and American ginseng extract and the matter of the auxiliary material
The ratio between amount is 22: 1；It is highly preferred that the auxiliary material is in solid dispersible carrier, starch, magnesium stearate or microcrystalline cellulose
It is one or more；
Preferably, the composition is by the way that maca microcapsule powder, American ginseng extract and solid dispersible carrier are mixed, through solid point
Dissipate and be prepared；It is highly preferred that the solid dispersible carrier is selected from PVP K30, PEG-4000, poloxamer-188, xylitol
Or the one or more in sorbierite.
6. composition according to claim 1, it is characterised in that the composition is capsule, tablet, granule or micro-
Pill.
7. the preparation method of composition as any one of claim 1 to 6, the preparation method comprises the following steps：
(1) maca is crushed into broken wall through low-temperature airflow, then included obtained maca fine powder successively, solid disperses, micro-
Encapsulated processing, is made maca microcapsule powder；
(2) cauline leaf and stem tuber of American Ginseng are taken, uses concentration of volume percent to be extracted for 70~80% ethanol water hot dipping,
Leaching liquor is concentrated, concentrate is adsorbed with macroporous absorbent resin, then successively using 70~80% ethanol water, washing
It is de-, eluent is merged, concentrated, is dried, American ginseng extract is obtained；
(3) by maca microcapsule powder, American ginseng extract, optional pharmaceutically acceptable auxiliary materials and mixing.
8. method according to claim 7, it is characterised in that in step (1), the low-temperature airflow is crushed in indifferent gas
Under body, carried out at a temperature of -20 DEG C, grinding particle size is 800~1200 mesh；
Preferably, in step (1), the inclusion is：Space net structure inclusion material is weighed, is added based on inclusion material weight
Enter 2~5 times of water, grinding is uniform, then adds maca fine powder, grind to form and dried after pasty state at 2~10 DEG C, obtain inclusion production
Thing；It is highly preferred that the one kind of the inclusion material in beta-schardinger dextrin, alpha-cyclodextrin, gamma-cyclodextrin or gas phase superfine silica gel powder
Or it is a variety of；
Preferably, in step (1), the microencapsulation is carried out using phase separation-coacervation；It is highly preferred that in the microencapsulation
One or more of the coating material used in sodium alginate, sodium carboxymethylcellulose, PEG or Arabic gum；
Preferably, step (3) is：Maca microcapsule powder, American ginseng extract, solid dispersible carrier are mixed, addition presses body in right amount
The ethanol water of product percent concentration meter 70~75%, strong stirring 4~5 hours makes it be uniformly dispersed, and obtains solid and disperses
Body；Wherein, the quality of solid dispersible carrier is the 0.5~5% of maca microcapsule powder and American ginseng extract gross mass.
9. according to the method for claim 7 or 8, the step of wherein methods described also includes composition preparation is made；It is excellent
Selection of land, the preparation is capsule, tablet, granule or micropill preparation.
10. composition according to any one of claim 1 to 6 or the side according to any one of claim 7 to 9
Composition prepared by method is preparing medicine, health food, feature ordinary food, the special medicine food or special of fatigue-relieving
Purposes in dietary food product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710281445.5A CN107137443A (en) | 2017-04-26 | 2017-04-26 | A kind of composition for relieving fatigue, preparation method and applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710281445.5A CN107137443A (en) | 2017-04-26 | 2017-04-26 | A kind of composition for relieving fatigue, preparation method and applications |
Publications (1)
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| CN107137443A true CN107137443A (en) | 2017-09-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710281445.5A Pending CN107137443A (en) | 2017-04-26 | 2017-04-26 | A kind of composition for relieving fatigue, preparation method and applications |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019154161A1 (en) * | 2018-02-06 | 2019-08-15 | 海文健康科技（深圳）有限公司 | Pharmaceutical composition for treating male diseases, and preparation method therefor and use thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104489681A (en) * | 2014-12-30 | 2015-04-08 | 广州健码医药生物科技有限公司 | Composition with function of enhancing immunity, health product and preparation method thereof |
| CN106074444A (en) * | 2016-06-21 | 2016-11-09 | 丽江久康生物科技有限公司 | Lepidinm meyenii Walp microcapsule powder as well as preparation method and application thereof |
-
2017
- 2017-04-26 CN CN201710281445.5A patent/CN107137443A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104489681A (en) * | 2014-12-30 | 2015-04-08 | 广州健码医药生物科技有限公司 | Composition with function of enhancing immunity, health product and preparation method thereof |
| CN106074444A (en) * | 2016-06-21 | 2016-11-09 | 丽江久康生物科技有限公司 | Lepidinm meyenii Walp microcapsule powder as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 宋月等: "西洋参茎叶中皂苷、多糖联合提取工艺研究", 《中华中医药学刊》 * |
| 金文闻等: "玛咖和西洋参皂苷合用对小鼠免疫功能的影响", 《中国新药杂志》 * |
| 高珊等: "玛咖与西洋参缓解体力疲劳作用对比试验研究", 《实验动物科学与管理》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019154161A1 (en) * | 2018-02-06 | 2019-08-15 | 海文健康科技（深圳）有限公司 | Pharmaceutical composition for treating male diseases, and preparation method therefor and use thereof |
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Application publication date: 20170908 |