CN107136127A - Salt tolerant composite probiotics preparations and preparation method thereof - Google Patents
Salt tolerant composite probiotics preparations and preparation method thereof Download PDFInfo
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- CN107136127A CN107136127A CN201710178207.1A CN201710178207A CN107136127A CN 107136127 A CN107136127 A CN 107136127A CN 201710178207 A CN201710178207 A CN 201710178207A CN 107136127 A CN107136127 A CN 107136127A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
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Abstract
Salt tolerant composite probiotics preparations of the present invention are related to a kind of containing the preparation for adding bacterial cultures, mycelia or other analogs, more particularly to a kind of salt tolerant composite probiotics preparations for agricultural.Its purpose is to provide it is a kind of have complementary functions, the salt tolerant composite probiotics preparations that act synergistically and preparation method thereof.Salt tolerant composite probiotics preparations of the present invention include 83 88 parts of 58 parts of molasses, 58 parts of salt tolerant compound probiotic strain, 0.5 0.8 parts of wheat bran, 0.05 0.1 parts of phosphoamide, 0.05 0.1 parts of NaCl, 0.01 0.05 parts of tealeaves, 0.05 0.1 parts of compound amino acid, 0.1 0.2 parts of composite trace element, 0.05 0.1 parts of B B-complex, 0.05 0.1 parts of Chinese herbal medicine and water.It can optimize farmland ecological environment after the salt tolerant composite probiotics preparations use of the present invention, safeguard that soil microenvironment is balanced.The present invention is used for field of agricultural cultivation.
Description
Technical field
Contain the preparation for adding bacterial cultures, mycelia or other analogs, more particularly to one the present invention relates to a kind of
Plant the salt tolerant composite probiotics preparations for agricultural.
Background technology
Country's probiotics preparation is generally single microbial inoculum or directionality function microbial inoculum at present, and single microbial inoculum is to realize that certain is single
Effect is preferable during function.But for agriculture complicated environment, single microbial inoculum is not often when realizing its function value also
Just it is difficult to survive in soil, has a strong impact on using effect.
According to the understanding in the market equivalents, it is found that it always contains except the microbial bacterial agent for the organic fertilizer that ferments
Amount is higher outer, and its total plate count of the microbial inoculum applied in farmland production is generally relatively low.
The content of the invention
The present invention is in order to solve the above-mentioned technical problem and there is provided a kind of compound benefit of salt tolerant for having complementary functions, acting synergistically
Probiotics preparation and preparation method thereof.
The present invention relates to a kind of salt tolerant composite probiotics preparations, the probiotics preparation includes according to mass fraction：Molasses
5-8 parts, 5-8 parts of salt tolerant compound probiotic strain, 0.5-0.8 parts of wheat bran, 0.05-0.1 parts of phosphoamide, 0.05-0.1 parts of NaCl,
0.01-0.05 parts of tealeaves, 0.05-0.1 parts of compound amino acid, 0.1-0.2 parts of composite trace element, B B-complex 0.05-0.1
83-88 parts of part, 0.05-0.1 parts of Chinese herbal medicine and water.
Preferably, the salt tolerant compound probiotic strain includes lactobacillus acidophilus (Lactobacillus
Acidophilus), methamidophos (Zygosaccharomyces rouxii), bacillus subtilis (Bacillus
Subtilis (Ehrenberg) Cohn), Rhodopseudomonas palustris (Rhodop seudanonas palustris), thermophilic salt unwrapping wire
Bacterium, Lactobacillus casei (L.casei), Paenibacillus polymyxa (Paenibacillus polymyxa) and saccharomyces cerevisiae (Sac-
charomyces cerevisiae)。
Preferably, the thermophilic salt actinomyces include rose pink mold cyst bacterium (Streptosporangium roseum), newly added
The polymorphic actinomyces in slope (Actinopolymorpha singaporensis) and salt tolerant nocardia (Nocardiopsis
halotolerans Al-Zarban et al)。
Preferably, the Chinese herbal medicine includes Radix Codonopsis, Radix Isatidis, the Radix Astragali and matrimony vine.
Preferably, Radix Codonopsis, Radix Isatidis, the Radix Astragali, the mass ratio of matrimony vine are 1 in the Chinese herbal medicine:1:1:1.
The invention further relates to a kind of preparation method of salt tolerant composite probiotics preparations, it the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses, salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tea as needed
Leaf, compound amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add 30%~60%, the Ran Houjia of step (1) reclaimed water gross mass
Enter phosphoamide, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of 1~2d of fermentation in fermentation tank, be then transferred to normal temperature in fermenter
Ferment 10~15d, that is, salt tolerant composite probiotics preparations are made.
Take out zymotic fluid to be detected, if Testing index meets agriculture microbial bacterial agent standard, by the hair in fermentation tank
Yeast-like fungi liquid, which is transferred in holding vessel, is ready for filling finished product；If Testing index does not meet agriculture microbial bacterial agent standard, after
It is continuous to be fermented.
Preferably, step (2) the high temperature sterilizing is 85~90 DEG C, 30~45min.
Preferably, the salt tolerant compound probiotic strain includes lactobacillus acidophilus (Lactobacillus
Acidophilus), methamidophos (Zygosaccharomyces rouxii), bacillus subtilis (Bacillus
Subtilis (Ehrenberg) Cohn), Rhodopseudomonas palustris (Rhodop seudanonas palustris), thermophilic salt unwrapping wire
Bacterium, Lactobacillus casei (L.casei), Paenibacillus polymyxa (Paenibacillus polymyxa) and saccharomyces cerevisiae (Sac-
charomyces cerevisiae)。
Preferably, the thermophilic salt actinomyces include rose pink mold cyst bacterium (Streptosporangium roseum), newly added
The polymorphic actinomyces in slope (Actinopolymorpha singaporensis) and salt tolerant nocardia (Nocardiopsis
halotolerans Al-Zarban et al)。
Preferably, the Chinese herbal medicine includes Radix Codonopsis, Radix Isatidis, the Radix Astragali and matrimony vine.
Salt tolerant composite probiotics preparations difference from prior art of the present invention is：
1. there is provided a kind of preparation side of compound probiotic microbial inoculum for the present situation of the invention for the deterioration of current agroecological environment
Method, it is optimal to have given play to because possessing the flora of a variety of functions, therefore the characteristics of the flora of difference in functionality plays respective in soil
Effect.
2. there is the flora for suppressing pathogen in the compound probiotic microbial inoculum of the present invention, the flora, promising for having decomposing organic matter
Various microorganisms create the flora of nutrition, also have the flora for promoting crop root to absorb, and a variety of floras are fought jointly.
3. farmland ecological environment can be optimized after the salt tolerant composite probiotics preparations use of the present invention, soil micro-ecosystem is safeguarded
Environmental balance.
Embodiment
The salt tolerant composite probiotics preparations of the present invention are further described by following examples and checking test.
Embodiment 1
The preparation method of the salt tolerant composite probiotics preparations of the present embodiment, the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses, salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tea as needed
Leaf, compound amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add the 30% of step (1) reclaimed water gross mass, then add phosphinylidyne
Amine, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of fermentation 1d in fermentation tank, be then transferred to normal temperature in fermenter and send out
Ferment 12d；
(5) take out zymotic fluid and detected that Testing index meets agriculture microbial bacterial agent standard, that is, the compound benefit of salt tolerant is made
Probiotics preparation, the zymocyte liquid in fermentation tank is transferred in holding vessel and is ready for filling finished product.
The species and amount of each raw material of the present embodiment are as shown in table 1, table 2.
Embodiment 2
The preparation method of the salt tolerant composite probiotics preparations of the present embodiment, the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses, salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tea as needed
Leaf, compound amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add the 60% of step (1) reclaimed water gross mass, then add phosphinylidyne
Amine, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of fermentation 2d in fermentation tank, be then transferred to normal temperature in fermenter and send out
Ferment 10d；
(5) take out zymotic fluid and detected that Testing index meets agriculture microbial bacterial agent standard, that is, the compound benefit of salt tolerant is made
Probiotics preparation, the zymocyte liquid in fermentation tank is transferred in holding vessel and is ready for filling finished product.
The species and amount of each raw material of the present embodiment are as shown in table 1, table 2.
Embodiment 3
The preparation method of the salt tolerant composite probiotics preparations of the present embodiment, the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses, salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tea as needed
Leaf, compound amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add the 40% of step (1) reclaimed water gross mass, then add phosphinylidyne
Amine, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of fermentation 1d in fermentation tank, be then transferred to normal temperature in fermenter and send out
Ferment 10d；
(5) take out zymotic fluid to be detected, Testing index does not meet agriculture microbial bacterial agent standard, return to fermenter relaying
Supervention ferment 1d, that is, be made salt tolerant composite probiotics preparations.
The species and amount of each raw material of the present embodiment are as shown in table 1, table 2.
Embodiment 4
The preparation method of the salt tolerant composite probiotics preparations of the present embodiment, the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses, salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tea as needed
Leaf, compound amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add the 50% of step (1) reclaimed water gross mass, then add phosphinylidyne
Amine, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of fermentation 1d in fermentation tank, be then transferred to normal temperature in fermenter and send out
Ferment 10d, that is, be made salt tolerant composite probiotics preparations.
The species and amount of each raw material of the present embodiment are as shown in table 1, table 2.
Embodiment 5
The preparation method of the salt tolerant composite probiotics preparations of the present embodiment, the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses, salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tea as needed
Leaf, compound amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add the 50% of step (1) reclaimed water gross mass, then add phosphinylidyne
Amine, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of fermentation 1d in fermentation tank, be then transferred to normal temperature in fermenter and send out
Ferment 15d, that is, be made salt tolerant composite probiotics preparations.
The species and amount of each raw material of the present embodiment are as shown in table 1, table 2.
Compound amino acid is purchased from Henan Xing Yuan chemical products Co., Ltd, composite trace element purchase in above example 1-5
From Shanghai De Bang animal husbandry Co., Ltd, B B-complex is purchased from Hebei fast-growing fertilizer Science and Technology Ltd..
The amount (g) of each raw material in the embodiment 1-5 of table 1
The selection (g) of salt tolerant compound probiotic strain and Chinese herbal medicine in the embodiment 1-5 of table 2
Note：Do not filled out as nothing in above table.
In Guizhou Xiuwen County, in the presence of this preparation, soil organic matter content has reached 2%, soil granular by 0.7%
Substantially, geobiont, pest natural enemy, Bird's Nest content substantially increase structure.
In the Experimental Base of Changping County, Beijing, in the presence of this preparation, geobiont richness is significantly improved, and uses spade
Arbitrarily shovel, it is possible to find some small earthworms in soil, soil surface has an obvious mycelia, soil organic matter content by
0.6% has reached 1.7%, and a pin, which is stepped down, can obvious vestige, and soil mellowness is significantly improved.
It can optimize farmland ecological environment after the salt tolerant composite probiotics preparations use for illustrating the present invention, safeguard the micro- life of soil
State environmental balance.
Although the foregoing describing the embodiment of the present invention, it will be appreciated by those of skill in the art that these
It is merely illustrative of, protection scope of the present invention is defined by the appended claims.Those skilled in the art is not carrying on the back
On the premise of principle and essence from the present invention, various changes or modifications can be made to these embodiments, but these are changed
Protection scope of the present invention is each fallen within modification.
Claims (10)
1. a kind of salt tolerant composite probiotics preparations, it is characterised in that the probiotics preparation includes according to mass fraction：Molasses 5-
8 parts, 5-8 parts of salt tolerant compound probiotic strain, 0.5-0.8 parts of wheat bran, 0.05-0.1 parts of phosphoamide, 0.05-0.1 parts of NaCl, tea
0.01-0.05 parts of leaf, 0.05-0.1 parts of compound amino acid, 0.1-0.2 parts of composite trace element, B B-complex 0.05-0.1
83-88 parts of part, 0.05-0.1 parts of Chinese herbal medicine and water.
2. salt tolerant composite probiotics preparations according to claim 1, it is characterised in that：The salt tolerant compound probiotic strain
Including lactobacillus acidophilus, methamidophos, bacillus subtilis, Rhodopseudomonas palustris, thermophilic salt actinomyces, Lactobacillus casei,
Paenibacillus polymyxa and saccharomyces cerevisiae.
3. salt tolerant composite probiotics preparations according to claim 2, it is characterised in that：The thermophilic salt actinomyces include rose
Pink mold cyst bacterium, the polymorphic actinomyces of Singapore and salt tolerant nocardia.
4. salt tolerant composite probiotics preparations according to claim 1, it is characterised in that：The Chinese herbal medicine includes Radix Codonopsis, plate
Lan Gen, the Radix Astragali and matrimony vine.
5. salt tolerant composite probiotics preparations according to claim 4, it is characterised in that：Radix Codonopsis, plate are blue in the Chinese herbal medicine
Root, the Radix Astragali, the mass ratio of matrimony vine are 1:1:1:1.
6. a kind of preparation method of salt tolerant composite probiotics preparations, it is characterised in that：It the described method comprises the following steps：
(1) raw material is weighed：Weigh molasses as needed, it is salt tolerant compound probiotic strain, wheat bran, phosphoamide, NaCl, tealeaves, multiple
Close amino acid, composite trace element, B B-complex, Chinese herbal medicine and water；
(2) molasses and wheat bran are subjected to high-temperature sterilization, add the 30%~60% of step (1) reclaimed water gross mass, then add carbon
Acid amides, NaCl, tealeaves, compound amino acid, composite trace element, B B-complex and Chinese herbal medicine；
(3) salt tolerant compound probiotic strain is added, remaining water is added while stirring；
(4) continue to stir after 30min, be transferred to 38 DEG C of 1~2d of fermentation in fermentation tank, be then transferred to normal temperature fermentation in fermenter
10~15d, that is, be made salt tolerant composite probiotics preparations.
7. salt tolerant composite probiotics preparations according to claim 6, it is characterised in that：Step (2) the high temperature sterilizing
For 85~90 DEG C, 30~45min.
8. salt tolerant composite probiotics preparations according to claim 7, it is characterised in that：The salt tolerant compound probiotic strain
Including lactobacillus acidophilus, methamidophos, bacillus subtilis, Rhodopseudomonas palustris, thermophilic salt actinomyces, Lactobacillus casei,
Paenibacillus polymyxa and saccharomyces cerevisiae.
9. salt tolerant composite probiotics preparations according to claim 8, it is characterised in that：The thermophilic salt actinomyces include rose
Pink mold cyst bacterium, the polymorphic actinomyces of Singapore and salt tolerant nocardia.
10. salt tolerant composite probiotics preparations according to claim 7, it is characterised in that：The Chinese herbal medicine includes Radix Codonopsis, plate
Lan Gen, the Radix Astragali and matrimony vine.
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Cited By (7)
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| CN107382556A (en) * | 2017-09-15 | 2017-11-24 | 北京百丰天下生物科技有限公司 | Complex microorganism foliage bacterial fertilizer and preparation method thereof |
| CN108576072A (en) * | 2018-05-07 | 2018-09-28 | 北京百丰天下生物科技有限公司 | It is exclusively used in the biology control worm agent and preparation method thereof of chewing type insect prevention and control |
| CN108640750A (en) * | 2018-06-27 | 2018-10-12 | 齐河百丰生物技术有限公司 | A kind of ecotype multielement liquid bacterial fertilizer and preparation method thereof |
| CN108902202A (en) * | 2018-05-07 | 2018-11-30 | 北京百丰天下生物科技有限公司 | It is exclusively used in the biological prevention and control agent and preparation method thereof of preventing and controlling subterranean pest |
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| CN109836285A (en) * | 2017-11-24 | 2019-06-04 | 北京百丰天下生物科技有限公司 | A kind of preparation method of the geobiont repair agent of anti-continuous cropping |
| CN108576072A (en) * | 2018-05-07 | 2018-09-28 | 北京百丰天下生物科技有限公司 | It is exclusively used in the biology control worm agent and preparation method thereof of chewing type insect prevention and control |
| CN108902202A (en) * | 2018-05-07 | 2018-11-30 | 北京百丰天下生物科技有限公司 | It is exclusively used in the biological prevention and control agent and preparation method thereof of preventing and controlling subterranean pest |
| CN108640750A (en) * | 2018-06-27 | 2018-10-12 | 齐河百丰生物技术有限公司 | A kind of ecotype multielement liquid bacterial fertilizer and preparation method thereof |
| CN109156482A (en) * | 2018-09-27 | 2019-01-08 | 宁夏普惠农业生物科技有限公司 | Plant growth promoter and preparation method thereof and application method |
| CN114009586A (en) * | 2021-11-19 | 2022-02-08 | 广东容大生物股份有限公司 | Development and application of composite probiotic preparation with high intestinal tract colonization rate |
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