CN107135927B - Cuttage and rapid propagation method for alum roots - Google Patents
Cuttage and rapid propagation method for alum roots Download PDFInfo
- Publication number
- CN107135927B CN107135927B CN201710440334.4A CN201710440334A CN107135927B CN 107135927 B CN107135927 B CN 107135927B CN 201710440334 A CN201710440334 A CN 201710440334A CN 107135927 B CN107135927 B CN 107135927B
- Authority
- CN
- China
- Prior art keywords
- cutting
- cuttage
- alum
- percent
- female parent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/22—Shades or blinds for greenhouses, or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
A method for quickly culturing the alum root by cuttage includes such steps as culturing the alum root in container in a plastic shed with shading rate of 50% and temp (6-35 deg.C). The cutting medium is formed by mixing coconut chaff, turf, vermiculite and perlite according to the volume ratio of 2-3: 1: 0.5: 0.2-0.5; the cuttage time is from 3 ten days to 10 middle days of the month; cutting the base part of the petiole of the mature blade of the alum root mother plant together with the young bud to be used as a cutting shoot; after disinfection treatment, the cutting slips are dipped in rooting agent containing naphthylacetic acid and indolebutyric acid and then are cut in a hole tray of the forest, the hole tray is placed on a seedbed in the greenhouse, a small arched shed is arranged on the seedbed, the shading rate in the small arched shed is 70-80%, and the relative humidity of air is 80-90%; and (5) hardening seedlings. By adopting the method, the seedling rate reaches more than 95 percent after 39-58 days of cuttage, the rooting condition is good, the cost is low, the operation is simple, the seedling character is stable, and the large-scale and standardized production of the seedlings can be realized.
Description
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a convenient and efficient alum root cutting propagation method.
Background
Alum root (Heuchera micrantha), also known as coral bell, is a perennial herb of the genus alum of the family Saxifragaceae, distributed in the middle of America, leafy basal, broad-egg-shaped, with a basal heart-shaped or wedge-shaped, with a palm-shaped, shallow to deep-cracked, wavy edge, preferring neutral, acid-biased, loose, fertile and well-draining soils, preferring yang and resisting yin. The alum root is a common under-forest ornamental plant in European and American gardens due to various excellent characteristics of color leaves (the leaf color has various colors such as red, purple, yellow, bronze, green, orange and the like), cold resistance, drought resistance and the like. In recent years, with the development of ornamental and horticultural industries in China, the attention of people to new and excellent flower varieties is improved at present, more and more alum root varieties are introduced into China, and are gradually applied to plant configuration of flower beds, flower beds and flower zones to obtain the favor of landscaping and household gardening markets.
The conventional alum root propagation method comprises sowing, plant division and tissue culture. Most alum root varieties are difficult to fruit; the propagation coefficient of the plant division is extremely low, large-scale production cannot be realized, and the size of the plant after the plant division is different, so that the uniformity is influenced; the tissue culture propagation cost is high, the process is complicated, the period is long, and a large number of seedlings are difficult to form quickly.
At present, the method of the present invention has not been retrieved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides the alum root cutting rapid propagation method which is suitable for large-scale and standardized production, simple to operate, high in propagation speed and low in cost.
The technical scheme of the invention is as follows:
1. a method for cuttage and rapid propagation of alum root comprises the following steps:
(1) female parent garden construction and female parent management
① construction of female parent garden
Establishing a alum root ear-picking female parent garden in a plastic greenhouse with a shading rate of 50%, and controlling the temperature in the plastic greenhouse with the shading rate of 50% to be 6-35 ℃;
② mother of parents
A. Planting the alum root mother seeds in a flowerpot filled with a mixed substrate for 2-3 months, wherein the mixed substrate is formed by applying 3kg of controlled release fertilizer to each cubic meter of the culture substrate and mixing; the culture medium is formed by mixing coconut coir, turf and perlite, and the volume ratio of the coconut coir to the turf to the perlite is 1-2: 1: 0.2-0.5; in terms of mass fraction, the total nitrogen and the soluble phosphorus in the controlled release fertilizer are respectively 18.00 percent and P2O5Is counted as6.00% soluble potassium as K212.00 percent of O, 1.80 percent of magnesium, 7.00 percent of sulfur, 0.05 percent of copper, 0.45 percent of iron, 0.05 percent of manganese, 0.05 percent of zinc and 0.0005 percent of molybdenum;
B. placing the flowerpot planted with the female parent of the alum root in the plastic greenhouse described in the step (1) ①, watering the female parent once every 2-3 days in the growth period of 3-10 months and stopping watering in rainy days, watering the female parent once every 4-6 days in the dormancy period of 2 months in the following month from 11 months to the next year and stopping watering in rainy days, topdressing once every 6-8 months and stopping topdressing in the dormancy period of the female parent in 2 months in the following month from 11 months to the next year, wherein the topdressing method comprises the steps of uniformly scattering the controlled release fertilizer in the step (1) ② A on the surface of a mixed matrix in the flowerpot, applying the controlled release fertilizer in an amount of 12-20 g/pot, and covering the surface with turf or coconut coir;
(2) preparation before cutting
Filling a cuttage matrix in a tree hole tray with 72 holes, and disinfecting the cuttage matrix 2-3 hours before cuttage by using 1000-1200 times of 70% thiophanate methyl wettable powder or 1000-1200 times of 50% carbendazim wettable powder, wherein the cuttage matrix is formed by mixing coconut chaff, grass peat, vermiculite and perlite according to the volume ratio of 2-3: 1: 0.5: 0.2-0.5 of coconut chaff, grass peat, vermiculite and perlite;
(3) cutting collection
Cutting the base part of the petiole of the mature blade of the alum root parent and 1-2 buds together to be used as cutting slips, wherein each cutting slip is provided with the mature blade, the mature blade in each cutting slip is controlled to be 1-3, and the cut under each cutting slip is cut flat;
(4) cutting
The cutting time is 3-10 middle of the month, and the cutting slips adopted in the step (3) are put into 1000-1200 times of 70% thiophanate methyl wettable powder or 1000-1200 times of 50% carbendazim wettable powder for soaking and disinfection for 5-10 minutes; then quickly dipping the base parts of the cutting slips after soaking and disinfection with 250-1000 ppm rooting agent for 5-10 seconds, cutting the cutting slips into a 72-hole forest hole tray subjected to disinfection treatment on a cutting medium in the step (2), cutting 1 cutting slip in each hole, immediately watering thoroughly after cutting is finished, and then entering a cutting slip maintenance stage; the 250-1000 ppm rooting agent is a solution containing naphthylacetic acid and indolebutyric acid, and the weight ratio of the naphthylacetic acid to the indolebutyric acid is 2-4: 1;
(5) maintenance of cutting
Setting a seedbed in the plastic greenhouse with the shading rate of 50% in the step (1) ①, placing a hole tray of 72 holes of forest trees inserted with cutting slips on the seedbed, installing a small plastic film arched shed on the seedbed, covering a shading net on the small plastic film arched shed to enable the shading rate in the small plastic film arched shed to be 70% -80%, controlling the relative air humidity in the small plastic film arched shed to be 80% -90%, and hardening seedlings after the root systems of the cutting slips grow to the hole tray holes, wherein the seedling hardening method comprises the steps of reducing the shading rate in the small plastic film arched shed to 60% -65%, reducing the relative air humidity in the small plastic film arched shed to 50% -60%, and after 3-4 days, completely opening the plastic films on the small plastic film arched shed and hardening the seedlings for 4-6 days to reach the standard of seedling emergence.
2. According to the technical scheme 1, the jardiniere for planting the alum root female parent in the step (1) is planted in a jardiniere with a pot diameter of 21-25 cm and a pot height of 16-20 cm, and the placement density of the alum root female flower pot is 3500-4000 pots/mu.
3. According to the technical scheme 1, in the 72-hole forest tree hole tray in the step (2), each hole is 55mm deep, and the volume of each hole is 45 cubic centimeters.
4. According to the technical scheme 1, in the step (1) ② A, the culture medium has a pH value of 6.0-6.5 and an EC value of 1.3-1.5 ms/cm, wherein the fiber length of coconut coir is 10-36 mm, the fiber length of turf is within 30mm, and the particle size of perlite is 2-4 mm.
5. According to the technical scheme 1, in the step (2), the pH value of the cutting medium is 6.0-6.5, the EC value is 0.5-0.7 ms/cm, the fiber length of coconut coir is 10-36 mm, the fiber length of grass carbon is within 10mm, the particle size of vermiculite is 1-3 mm, and the particle size of perlite is 2-4 mm.
6. According to the technical scheme 1, in the cuttage in the step (4), if mature leaves of two adjacent cuttings in a tree hole tray of 72 holes are mutually shielded, 1/2-2/3 of the mature leaves are cut off until the mature leaves of two adjacent cuttings in the tree hole tray of 72 holes are mutually not shielded.
7. According to the technical scheme 1, in the step (4), the cutting depth is based on the condition that the buds at the base parts of the leaf stalks of the cutting slips are exposed out of the cutting medium.
8. According to the technical scheme 1, in the step (3) of cutting shoot collection, the cutting shoots are put into a container filled with clear water for moisture preservation after being cut off from a parent.
9. According to the method for the cuttage and the rapid propagation of the alum root in the technical scheme 1, in the step (3), the cuttage of the adopted cutting slips is finished within 2 hours.
10. According to the technical scheme 1, the alum root female parent is updated every 3-4 years in the step (1).
Compared with the prior art, the invention has the beneficial effects that:
at present, the alum root propagation is mainly produced in a large scale by a tissue culture mode, and the production cost is higher. The method firstly researches a alum root cutting rapid propagation method suitable for large-scale and standardized production through a large number of tests, comprises a whole set of large-scale and standardized rapid propagation measures such as female parent cultivation, cutting shoot selection and treatment, cutting shoot rooting environment control and the like, can adapt to the climate environment of most areas in China, has good rooting condition, high yield of more than 95 percent, simple operation, low cost and high propagation speed, only needs 39-58 days from cutting to forming of commodity seedlings, and has stable genetic characters of seedlings and high propagation coefficient. 60-100 cuttings are harvested per female parent year, and the method can be developed in large scale in combination with potted flower production, and has good market prospect.
The method adopts the young buds at the base part of the mature leaves as the cutting slips, and the technical key point is matched with proper cutting measures, so that the cutting propagation of the alum roots is promoted, the cutting survival rate reaches more than 95 percent, the propagation period is short, the problems that the alum root variety is hard to seed, the plant division propagation coefficient is extremely low, the large-scale production cannot be realized, and the plants are different in size after the plant division, so that the uniformity is influenced are solved; the tissue culture propagation cost is high, the process is complicated, the period is long, and a large number of seedlings are difficult to form quickly.
Detailed Description
The materials used in the following examples are commercially available, and the examples are not specifically described as conventional.
Example 1
Cuttage and rapid propagation of alum root variety' Ma Lei Du
1 female parent nursery construction and female parent management
1.1 construction of female parent nursery
The alum root mother ear cutting garden is built in a plastic greenhouse which is well ventilated and is not easy to accumulate water, the span of the greenhouse is 8 meters, the bay is 4 meters, the shoulder height is 4 meters, a sunshade net with the shading rate of 50 percent is manually opened and closed is arranged inside the greenhouse, the ground of the greenhouse is covered by black ground cloth to prevent weeds from growing, a drainage ditch with the depth of 30cm and the width of 40cm is arranged every 8 meters (every span) of the ground of the greenhouse, and the bottom of the drainage ditch is provided with 3 percent of gradient to facilitate the drainage of the accumulated water. The temperature in the plastic greenhouse with the shading rate of 50% is controlled to be 6-35 ℃, the highest temperature in summer is lower than 35 ℃, and the lowest temperature in winter is higher than 6 ℃.
1.2 maternal management
A. Planting alum root 'maca bud' female parent in a flowerpot of 7.5L (pot diameter is 25cm, pot height is 20cm) PP material filled with a mixed matrix for 2-3 months each year, wherein the mixed matrix is formed by applying 3kg of controlled release fertilizer to each cubic meter of culture matrix, the culture matrix is formed by mixing coconut chaff, grass peat and perlite, the volume ratio of the coconut chaff to the grass peat to the perlite is 2: 1: 0.5, the pH value of the culture matrix is 6.0-6.5, the EC value is 1.3-1.5 ms/cm, the fiber length of the coconut chaff is 10-36 mm, the fiber length of the grass peat is within 30mm, the particle size of the perlite is 2-4 mm, and the alum root 'maca bud' female parent is updated every 4 years.
The controlled release fertilizer contains 18.00 percent of total nitrogen and P as soluble phosphorus2O56.00% of soluble potassium in terms of K212.00 percent of O, 1.80 percent of magnesium, 7.00 percent of sulfur, 0.05 percent of copper, 0.45 percent of iron, 0.05 percent of manganese, 0.05 percent of zinc and 0.0005 percent of molybdenum, 2.80 percent of ammonium nitrogen, 1.30 percent of nitrate nitrogen and 13.90 percent of amide nitrogen in the total nitrogen; the controlled release fertilizer is prepared by the American Simpleo corporationThe produced Abelia 18-6-12 arbor and shrub GA L-XEONE controlled release fertilizer (commercially available) are renewed every 4 years for the female parent of alum root 'Ma Lei Du'.
B. And (3) placing the flowerpot planted with the alum root female parent in the vinyl house in the step 1.1, wherein the placing density of the flowerpot is 3500 pots/mu. The alum root 'maca buds' are dry and do not resist water accumulation, in the growth period of the female parent in 3-10 months, water is permeated once every 2-3 days, and watering is stopped in rainy days; watering for permeating water once every 4-6 days in the period from 11 months to 2 months in the next year, and stopping watering in rainy days; topdressing is carried out once every 6-8 months, and the topdressing is stopped in the period from 11 months to 2 months in the next year; the top dressing method comprises the following steps: uniformly scattering the controlled release fertilizer in the step 1.2A on the surface of a mixed matrix in a flowerpot, wherein the application amount is 20 g/pot, and grass carbon with the fiber length within 10mm or coconut coir with the fiber length of 10-36 mm is used for surface covering, and the covering thickness is 5-10 mm; and stopping top dressing at the period of the female parent dormancy stage from 11 months every year to 2 months next year.
2 cutting
2.1 cuttage time
Suitable cutting time is 3 to 10 months of each year.
2.2 preparation before cuttage
The cuttage matrix is uniformly mixed, 72-hole forest tree hole trays are used for containing the cuttage matrix, the hole trays are 540 × 280mm (the length is × mm), the hole depth is 55mm, the volume of each hole is 45 cubic centimeters, the surfaces of the cuttage matrix are lightly pressed and are not too tightly pressed, the cuttage matrix is subjected to disinfection treatment by using 1200 times of 70% thiophanate methyl wettable powder 2 hours before cuttage, the cuttage matrix is formed by fully mixing coconut chaff, grass peat, vermiculite and perlite according to the volume ratio of 3: 1: 0.5, wherein the fiber length of the coconut chaff is 10-36 mm, the fiber length of the grass peat is within 10mm, the particle size of the vermiculite is 1-3 mm, and the particle size of the perlite is 2-4 mm, the pH value of the cuttage matrix is 6.0-6.5, and the EC value is 0.5-0.7 ms/cm.
2.3 cutting shoot Collection
Cutting the base part of a petiole of a alum root 'maca bud' mature leaf in a female parent garden together with 1-2 buds as a cutting slip (namely the base part of the petiole is provided with 1-2 buds), wherein each cutting slip is required to be provided with the mature leaf, the mature leaves in each cutting slip are controlled between 1-3 pieces, and the cut under the cutting slip is cut flat; more than 2 buds need to be divided into two cuttings, the cuttings are cut away from the parent body and then put into a container filled with clear water for moisture preservation, and the cuttings need to be cut within 2 hours.
2.4 cuttage
Specifically, in the ten days of 3 months, the whole cutting slips collected in the step 2.3 are placed into 1000 times of solution of 70% thiophanate methyl wettable powder for soaking and disinfection for 5 minutes, the cutting slips are taken out and dried slightly, then the base parts of the cutting slips after soaking and disinfection are dipped in 500ppm of rooting agent for 5-10 seconds, the cutting slips are planted in a 72-hole forest plug tray after the cutting substrates are disinfected in the step 2.2, 1 cutting slip is inserted into each hole, the cutting depth is based on the condition that the young buds of the base parts of the leaf stalks of the cutting slips are exposed out of the cutting substrates, if mature leaves of two adjacent cutting slips in the 72-hole forest plug tray are mutually shielded, 1/2-2/3 of the mature leaves are cut off until the mature leaves of two adjacent cutting slips in the 72-hole forest plug tray are mutually not shielded, water is immediately poured after cutting, then the cutting slips enter a constant volume stage, the rooting agent with 500ppm is a solution containing naphthylacetic acid and indolebutyric acid, the weight ratio of the naphthylacetic acid to indolebutyric acid is 3: 1, the preparation method of the rooting agent is characterized in that the method that the rooting agent is implemented by adding one thousand of water with the precision of the method that the method is one of the method that the method is implemented, the method is implemented, the method that the method is implemented, the method.
2.5 maintenance of cutting slips
Setting a seedbed in the plastic greenhouse with the shading rate of 50% in the step 1.1, placing a hole tray of 72-hole forest trees in which the cutting slips are inserted on the seedbed, installing a small plastic film arched shed on the seedbed for heat preservation and moisture preservation, covering a shading net on the small plastic film arched shed to ensure that the shading rate in the small plastic film arched shed is 70% -80%, and simultaneously controlling the relative humidity of air in the small plastic film arched shed to be 80% -90%. And (3) callus appears on the base part of the cutting shoot 10-12 days after cutting, rooting starts in 15-18 days, and hardening seedlings after the root system grows over the plug hole in 35-40 days, wherein the hardening seedling method comprises the following steps: reducing the shading rate in the small plastic film arched shed to 60-65%, and reducing the relative air humidity in the small plastic film arched shed to 50-60%; and after 3-4 days, completely lifting the plastic film on the plastic film small arched shed, and hardening the seedlings for 4-6 days to reach the standard of seedling emergence. The alum root and the maca bud take 42-50 days from cuttage to seedling formation, and the seedling formation rate averagely reaches 96.4%.
Example 2 cuttage and Rapid propagation of Alum root variety' plum pudding
Example 2 the remaining steps are the same as in example 1 except for the following measures, and are not described again.
1.2 maternal management
A. The alum root 'plum pudding' female parent is planted in a flowerpot made of 6L (the diameter of the flowerpot is 23cm, the height of the flowerpot is 18cm) PP material, the flowerpot is placed at a density of 3800 pots/mu, the culture medium is formed by mixing coconut chaff, turf and perlite according to the volume ratio of 2: 1: 0.2, the pH value of the culture medium is 6.0-6.5, and the EC value is 1.3-1.5 ms/cm.
B. The amount of the controlled release fertilizer applied to the female parent of the alum root 'plum pudding' every 6-8 months is 16 g/pot.
2.2 preparation before cuttage
The cuttage matrix is formed by fully mixing coconut coir, turf, vermiculite and perlite according to the volume ratio of 3: 1: 0.5: 0.2, the pH value of the cuttage matrix is 6.0-6.5, and the EC value is 0.5-0.7 ms/cm.
2.4 cuttage
The rooting agent solution which is quickly dipped at the base of the cutting slips of the alum root 'plum pudding' has the concentration of 250ppm, the weight ratio of the naphthylacetic acid to the indolebutyric acid is 4: 1, and the 250ppm rooting agent is prepared by weighing 0.20g of naphthylacetic acid and 0.05g of indolebutyric acid by using an electronic balance with the precision of one thousandth, putting the weighed materials into a small beaker, completely dissolving the materials by using a NaOH solution with the concentration of 1 g/L, transferring the solution into a measuring cylinder, and fixing the volume to L m by using water, so that the 250ppm rooting agent is obtained.
And (3) callus appears on the base parts of the cuttings 7-10 days after the alum root 'plum pudding' is subjected to cuttage, rooting starts on 12-17 days, and hardening seedlings after the hole tray holes are full of root systems on 32-38 days. The alum root 'plum pudding' needs 39-48 days from cuttage to seedling formation, and the seedling formation rate averagely reaches 98.7%.
Example 3 cuttage for the Alum root variety "tiramisu
Example 3 the remaining steps are the same as in example 1 except for the following measures, and are not described again.
1.2 maternal management
A. The alum root 'tiramisu' female parent is planted in a flowerpot made of PP material 4L (pot diameter 21cm, pot height 16cm), the flowerpot is placed at a density of 4000 pots/mu, the culture medium is formed by mixing coconut chaff, turf and perlite according to the volume ratio of 1: 0.2, the pH value of the culture medium is 6.0-6.5, and the EC value is 1.3-1.5 ms/cm. alum root 'tiramisu' female parent is updated once every 3 years.
B. The alum root 'tiramisu' female parent is applied with the controlled release fertilizer once every 6 to 8 months, and the application amount is 12 g/pot.
2.2 preparation before cuttage
And (3) disinfecting the cutting medium of the alum root 'tiramisu' by adopting 1000 times of 50% carbendazim wettable powder 3 hours before cutting. The cuttage matrix is formed by fully mixing coconut coir, turf, vermiculite and perlite according to the volume ratio of 2: 1: 0.5: 0.2 of coconut coir, turf, vermiculite and perlite, the pH value of the cuttage matrix is 6.0-6.5, and the EC value is 0.5-0.7 ms/cm.
2.4 cuttage
The concentration of a rooting agent solution for quickly dipping the base part of a cutting slip of alum root 'tiramisu' is 1000ppm, the weight ratio of naphthylacetic acid to indolebutyric acid is 2: 1, and the preparation method of the rooting agent with 1000ppm comprises the steps of weighing 0.40g of naphthylacetic acid and 0.20g of indolebutyric acid by using an electronic balance with one thousandth of precision, putting the weighed materials into a small beaker, completely dissolving the materials by using a NaOH solution with the concentration of 1 g/L, and transferring the solution into a measuring cylinder to be constant in volume by using water to 600m L, so that the rooting agent with 1000ppm is obtained.
And (3) callus appears at the base of the cutting slips 12-14 days after the alum root 'tiramisu' is cut, rooting starts on 21-25 days, and hardening seedlings after the hole tray holes are full of root systems on 42-48 days. The alum root 'tiramisu' requires 49-58 days from cuttage to seedling formation, and the seedling formation rate reaches 95.2 percent on average.
Although embodiments of the present invention have been shown and described, it should be noted that, for those skilled in the art, various modifications and improvements can be made without departing from the technical principle of the present invention, and these modifications and improvements should also be construed as the protection scope of the present invention.
Claims (10)
1. A alum root cutting rapid propagation method is characterized by comprising the following steps:
(1) female parent garden construction and female parent management
① construction of female parent garden
Establishing a alum root ear-picking female parent garden in a plastic greenhouse with 50% shading rate, and controlling the temperature in the plastic greenhouse with 50% shading rate to be between 6 and 35 ℃;
② mother of parents
A. Planting the alum root mother seeds in a flowerpot filled with a mixed substrate for 2-3 months, wherein the mixed substrate is formed by applying 3kg of controlled release fertilizer to each cubic meter of the culture substrate and mixing; the culture medium is formed by mixing coconut coir, turf and perlite, and the volume ratio of the coconut coir to the turf to the perlite is 1-2: 1: 0.2-0.5; in terms of mass fraction, the total nitrogen and the soluble phosphorus in the controlled release fertilizer are respectively 18.00 percent and P2O56.00% of soluble potassium in terms of K212.00 percent of O, 1.80 percent of magnesium, 7.00 percent of sulfur, 0.05 percent of copper, 0.45 percent of iron, 0.05 percent of manganese, 0.05 percent of zinc and 0.0005 percent of molybdenum;
B. placing the flowerpot planted with the female parent of the alum root in the plastic greenhouse described in the step (1) ①, watering the female parent once every 2-3 days in the growth period of 3-10 months and stopping watering in rainy days, watering the female parent once every 4-6 days in the dormancy period of 2 months in the following month from 11 months to the next year and stopping watering in rainy days, topdressing once every 6-8 months and stopping topdressing in the dormancy period of the female parent in 2 months in the following month from 11 months to the next year, wherein the topdressing method comprises the steps of uniformly scattering the controlled release fertilizer in the step (1) ② A on the surface of a mixed matrix in the flowerpot, applying the controlled release fertilizer in an amount of 12-20 g/pot, and covering the surface with turf or coconut coir;
(2) preparation before cutting
Filling a cuttage matrix in a tree hole tray with 72 holes, and disinfecting the cuttage matrix 2-3 hours before cuttage by using 1000-1200 times of 70% thiophanate methyl wettable powder or 1000-1200 times of 50% carbendazim wettable powder, wherein the cuttage matrix is formed by mixing coconut chaff, grass peat, vermiculite and perlite according to the volume ratio of 2-3: 1: 0.5: 0.2-0.5 of coconut chaff, grass peat, vermiculite and perlite;
(3) cutting collection
Cutting the base part of the petiole of the mature blade of the alum root parent and 1-2 buds together to be used as cutting slips, wherein each cutting slip is provided with the mature blade, the mature blade in each cutting slip is controlled to be 1-3, and the cut under each cutting slip is cut flat;
(4) cutting
The cutting time is 3-10 middle of the month, and the cutting slips adopted in the step (3) are put into 1000-1200 times of 70% thiophanate methyl wettable powder or 1000-1200 times of 50% carbendazim wettable powder for soaking and disinfection for 5-10 minutes; then quickly dipping the base parts of the cutting slips after soaking and disinfection with 250-1000 ppm rooting agent for 5-10 seconds, cutting the cutting slips into a 72-hole forest hole tray subjected to disinfection treatment on a cutting medium in the step (2), cutting 1 cutting slip in each hole, immediately watering thoroughly after cutting is finished, and then entering a cutting slip maintenance stage; the 250-1000 ppm rooting agent is a solution containing naphthylacetic acid and indolebutyric acid, and the weight ratio of the naphthylacetic acid to the indolebutyric acid is 2-4: 1;
(5) maintenance of cutting
Setting a seedbed in the plastic greenhouse with the shading rate of 50% in the step (1) ①, placing a hole tray of 72 holes of forest trees inserted with cutting slips on the seedbed, installing a small plastic film arched shed on the seedbed, covering a shading net on the small plastic film arched shed to enable the shading rate in the small plastic film arched shed to be 70% -80%, controlling the relative air humidity in the small plastic film arched shed to be 80% -90%, and hardening seedlings after the root systems of the cutting slips grow to the hole tray holes, wherein the seedling hardening method comprises the steps of reducing the shading rate in the small plastic film arched shed to 60% -65%, reducing the relative air humidity in the small plastic film arched shed to 50% -60%, and after 3-4 days, completely opening the plastic films on the small plastic film arched shed and hardening the seedlings for 4-6 days to reach the standard of seedling emergence.
2. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: and (2) planting the jardiniere for planting the alum root female parent in the step (1) by adopting a jardiniere with the pot diameter of 21-25 cm and the pot height of 16-20 cm, wherein the placement density of the alum root female parent jardiniere is 3500-4000 pots/mu.
3. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: and (3) in the 72-hole forest tree hole disc, the depth of each hole is 55mm, and the volume of each hole is 45 cubic centimeters.
4. The alum root cutting rapid propagation method according to claim 1, wherein the culture medium in the step (1) ② A has a pH value of 6.0-6.5 and an EC value of 1.3-1.5 ms/cm, wherein the fiber length of coconut coir is 10-36 mm, the fiber length of turf is within 30mm, and the particle size of perlite is 2-4 mm.
5. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: the pH value of the cuttage matrix in the step (2) is 6.0-6.5, the EC value is 0.5-0.7 ms/cm, the fiber length of coconut coir is 10-36 mm, the fiber length of grass carbon is within 10mm, the particle size of vermiculite is 1-3 mm, and the particle size of perlite is 2-4 mm.
6. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: in the cuttage in the step (4), if the mature leaves of two adjacent cuttings in the 72-hole forest plug tray are mutually shielded, cutting off 1/2-2/3 of the mature leaves until the mature leaves of two adjacent cuttings in the 72-hole forest plug tray are mutually not shielded.
7. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: in the step (4), the cutting depth is based on the condition that the buds at the base parts of the leaf stalks of the cutting slips are exposed out of the cutting medium.
8. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: in the step (3), the cutting slips are cut away from the parent bodies and then put into a container filled with clear water for moisture preservation.
9. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: in the step (3), the cutting is completed within 2 hours.
10. The method for cuttage and rapid propagation of alum roots according to claim 1, characterized in that: and (2) updating the alum root female parent in the step (1) every 3-4 years.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710440334.4A CN107135927B (en) | 2017-06-12 | 2017-06-12 | Cuttage and rapid propagation method for alum roots |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710440334.4A CN107135927B (en) | 2017-06-12 | 2017-06-12 | Cuttage and rapid propagation method for alum roots |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107135927A CN107135927A (en) | 2017-09-08 |
| CN107135927B true CN107135927B (en) | 2020-08-07 |
Family
ID=59781243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710440334.4A Active CN107135927B (en) | 2017-06-12 | 2017-06-12 | Cuttage and rapid propagation method for alum roots |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107135927B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853046A (en) * | 2017-10-26 | 2018-03-30 | 吉安市林业科学研究所 | A kind of wet-land pine tree industrial cuttage method for culturing seedlings |
| CN109197486A (en) * | 2018-07-16 | 2019-01-15 | 云南鑫源花卉种植有限公司 | A kind of laurustinus cuttage and seedling culture method |
| CN110892862B (en) * | 2019-12-12 | 2021-06-11 | 南昌工程学院 | Water culture cutting rapid propagation method for sassafras and application thereof |
| CN111492829B (en) * | 2020-04-30 | 2022-04-26 | 上海市农业科学院 | Open-field over-summer method for alum roots in Shanghai region |
| CN113508813B (en) * | 2021-03-26 | 2022-11-18 | 上海市农业科学院 | Rooting agent for alum root cutting and alum root cutting propagation method |
| CN115486363B (en) * | 2022-11-02 | 2024-03-26 | 岭南师范学院 | Seedling raising method for water planting of acanthus praecox leaves |
| CN115777361A (en) * | 2022-11-28 | 2023-03-14 | 北京林业大学 | Leaf cutting propagation method of Potentilla fragrans |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415275B (en) * | 2011-10-31 | 2013-08-21 | 浙江森禾种业股份有限公司 | Cuttage propagation method for juniperus horizontalis 'Wiltonii' |
| CN104871945B (en) * | 2015-06-02 | 2017-09-12 | 北京天卉苑花卉研究所 | A kind of method of alum root tissue culture propagation outside sprout-cultivating-bottle hardening |
| CN106069146A (en) * | 2016-07-08 | 2016-11-09 | 天津泰达绿化集团有限公司 | A kind of method for planting of vitriol root |
| CN106172000A (en) * | 2016-07-22 | 2016-12-07 | 上海应用技术学院 | Colorful Vegetation maltose vitriol root tissue culture and rapid propagation method |
-
2017
- 2017-06-12 CN CN201710440334.4A patent/CN107135927B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107135927A (en) | 2017-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107135927B (en) | Cuttage and rapid propagation method for alum roots | |
| CN102113445B (en) | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation | |
| CN105165512A (en) | Crown inclination controlling four-season cutting rapid propagation method for alnus formosana | |
| CN103283453A (en) | Large-scale cutting propagation method of ligustrum japonicum 'howardii' | |
| CN105532242A (en) | Cultivation method for promoting growth of paeonia ostii seedlings and achieving out-planting ahead of time | |
| CN110558172A (en) | Strawberry virus-free tissue culture seedling breeding method | |
| CN109906900B (en) | Potato protospecies breeding method | |
| CN111264328A (en) | Cultivation method of small bougainvillea spectabilis pot culture | |
| CN104221696A (en) | Jonquil large-scale atomizing cuttage seedling breeding method | |
| CN113179833A (en) | Method for efficient cuttage breeding of camellia oleifera seedlings in high-altitude areas | |
| CN106718763B (en) | Sedum short-shoot cutting cultivation method | |
| CN108605617B (en) | Sowing and seedling raising method for European mountain ash facility | |
| CN113079964A (en) | Seedling raising and planting method of limonium aureum | |
| CN107372070B (en) | Hardwood cutting seedling raising method based on Typha river fragrant oranges | |
| CN107155843B (en) | Improved method for industrially transplanting tissue culture seedlings of Chinese yams and cultivating potato seeds | |
| CN107409896B (en) | Sowing and seedling raising method for golden panda trees | |
| CN115380792A (en) | Greenhouse planting method for crocus sativus | |
| CN110313371B (en) | Method for cultivating plateau coronarium | |
| CN111972148B (en) | Cutting propagation and field regression method for medicinal endangered plant Jinyun scutellaria baicalensis | |
| CN110915577B (en) | Angelica keiskei seedling transplanting method | |
| CN110558130B (en) | Cutting method of cauliflower | |
| CN103749129A (en) | Method for asexually and rapidly propagating homalium hainanense | |
| CN109769676B (en) | Soilless culture method for promoting rapid flowering of rhododendron lapponicum good seedlings | |
| CN113331013A (en) | Method for sowing and forcing cultivation of pine needle lily | |
| CN105248232A (en) | Honeysuckle seedling raising method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |