CN107132228A - A kind of high flux phenotype research system of the full growth period information of rape - Google Patents
A kind of high flux phenotype research system of the full growth period information of rape Download PDFInfo
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- CN107132228A CN107132228A CN201710420085.2A CN201710420085A CN107132228A CN 107132228 A CN107132228 A CN 107132228A CN 201710420085 A CN201710420085 A CN 201710420085A CN 107132228 A CN107132228 A CN 107132228A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The present invention discloses a kind of high flux phenotype research system of the full growth period information of rape, including：Rapeseed breeding culture line, with an at least culturing rack, places the Culture basin with Electronic Numbering on the culturing rack, and equipped with the LED/light source of compensation illumination；Detecting system, including detection conveyer belt, the code reader for the imaging device of the Culture basin imaging to being conveyed on detection conveyer belt, and the identification Electronic Numbering；Control system, for analyzing image, and judges whether the growth in Culture basin is normal；Transport Machinery arm, the described Culture basin for being captured between rapeseed breeding culture line and detecting system, and put according to growth the position of the Culture basin.The present invention improves space availability ratio and operating efficiency using whole-course automation operation；The periodic detection in full growth period is carried out to rapeseed breeding, phenomenon of catching an illness is found as early as possible, loss is reduced, can also be judged whether to need to topdress to the rape of culture according to testing result.
Description
Technical field
The present invention relates to the full growth period breeding detection field of rape, and in particular to a kind of high pass of the full growth period information of rape
Scale type research system.
Background technology
Rape is one of common oil crops, and worldwide (China, Canada, India and European Union etc.) suffers from
Extensive plantation, is one of main source of world's edible vegetable oil and vegetable protein, and weight is occupied in international agriculture trade
Want status.China per capita can be few with cultivated area, reply chemical fertilizer, indiscriminate use of pesticide, the yield of raising rape unit cultivated area,
Quality, it is a kind of one of effective approach to carry out extensive greenhouse rapeseed breeding.Irregular be excessively used of chemical fertilizer causes
Arable land quality decline, and rape easily infects the diseases such as sclerotiniose, in China, sclerotiniose can cause the rape underproduction 10%~80%,
The use of chemical insecticide can be to certain prevention and control of having fallen ill, but there is certain hysteresis quality, and add and be produced into
This, pollutes to environment.Therefore in growth course, to the full growth cycle of rape (seedling stage, flower bud a kind of sedge phase, florescence, silique into
Ripe four periods of phase) nutrient physiology and defect information be monitored very and have and must have.
Crop breeding is, by Crop Improvement heredity, to obtain the technology of high yield and high quality kind, be that crop yield and quality are carried
One of main path risen.Biography can be efficiently solved by obtaining high-throughout crop breeding information using multi-source optical spectrum imaging data
The problems such as crop breeding ground artificial of uniting investigates low efficiency, poor in timeliness and standard disunity, provides efficiently certainly for breeding expert
Plan information.In recent years, spectrum and imaging technique are applied to agricultural production more and more widely.Hyperspectral technique has spectrum point
Resolution is high, the unique advantage of collection of illustrative plates, the problem of solving " having spectrum without figure " or " having figure without spectrum ", with its quick, lossless, nothing
Pollution and the low advantage of cost turn into agricultural production in recent years and develop modern analytical technique the most quick, high light spectrum image-forming
Instrument includes visible near-infrared and near infrared band, different wave bands is selected according to different research objects, because it can be each
Pixel provide more than ten, hundreds of or even thousands of wave bands (wave band is more), wavelength band be generally less than 10nm (spectral region is narrow), some
Sensor can provide object spectrum (wave band is continuous), the data volume of nearly singular integral in the range of 350-2500nm solar spectrum
The advantage such as big, beneficial to studied using Spectral Characteristics Analysis atural object, fine point using various Spectral matching models and atural object
Class and identification, study for crop phenotype and provide abundant data message.Imaging-PAM technology is ground as photosynthesis
The probe studied carefully, with specificity, high sensitivity the characteristics of, can quickly reflect crop physiology and ecology situation, and nothing can be realized
Detection is damaged, suffers from being widely applied in various fields such as mutant strain screening, pest and disease damage detection, phenotypic analyses.A variety of imaging skills
Art means can detect the spectral information of object with Overall Acquisition, while mutually comparing, effectively improve detection performance.
Traditional phenotypic analysis means rely primarily on the feature and character for visually observing and simply measuring plant, there is scale
The problems such as small, efficiency is low, error is big, practicality is weak, it is difficult to meet the reality of Functional Plant Genomics and Molecular design breeding
Demand.In order to fully excavate the information of genome to improve plant breeding efficiency, accelerate high flux phenotypic analysis technology and platform
Research it is significant.Between the past few decades, many scientists are by exquisite Phenotypic examination experimental design and diligent respect
The spirit of industry completes many broad scale research work.It is now, the phenotype research method automatically or semi-automatically changed, large-scale
Data processing method, can make it possible that large-scale production detection is applied in production practices.
The content of the invention
In order to overcome the limitation of above-mentioned existing breeding technique, it is an object of the invention to provide a kind of rape full growth period
The high flux phenotype research system of information, improves the automatization level of breeding, reduces cost of labor, improves breeding quality, shortens
Breeding time, the present invention has the advantages that the detection of multiple spectrum means is integrated, easy to use and detects high efficiency.
The research system of the present invention, the growth for periodically being monitored on-line rape full growth period using spectral imaging technology is believed
Breath, is analyzed by the spectral information acquired in the on-line monitoring to rape, can obtain the growth information of rape, be in oil
Dish, which catches an illness to provide effective prevention and control in early days and carry out variable according to rape nutrition condition, topdresses aspect there is provided quickly and accurately hand
Section.
Concrete technical scheme of the present invention is as follows：
A kind of high flux phenotype research system of the full growth period information of rape, including：
Rapeseed breeding culture line, with an at least culturing rack, the Culture basin with Electronic Numbering is placed on the culturing rack,
And equipped with the LED/light source of compensation illumination；
Detecting system, including detection conveyer belt, for the imaging device of the Culture basin imaging to being conveyed on detection conveyer belt,
And the code reader of the identification Electronic Numbering；
Control system, for analyzing image, and judges whether the growth in Culture basin is normal；
Transport Machinery arm, for capturing described Culture basin between rapeseed breeding culture line and detecting system, and according to rape
Upgrowth situation puts the position of the Culture basin.
In the present invention, the rapeseed breeding culture line, including culturing rack, the Culture basin with Electronic Numbering, compensatory light；
Culturing rack is sequentially placed in culturing room, and Culture basin is located on culturing rack；Equipped with artificial light, LED/light source compensation function, including
Red-light LED light source and blue light LED light source, supplement the feux rouges and blue light needed for rape photosynthesis, shorten the growth of rape respectively
Cycle.
Described Transport Machinery arm can be one or can coordinate the multiple of completion operation, and Culture basin is moved into detection passes
Send band；Normal individual is grown according to the instruction of control system sorting and caught an illness or underfed abnormal individuals, normal individual quilt
Send culturing rack back to, abnormal individuals are transferred to pending district.
The code reader is set to the identification to rapeseed plants in Culture basin, and by testing result by being wirelessly transferred
It is standby to be sent to control system, accomplish the identification to breeding individual, follow the trail of, trace to the source.
Described control system is the control centre entirely detected, the testing result of each several part will all pass through Wireless Transmitter
It is sent to control system；Control system analyzes the result to image scanning and detection, judges the individual crop of each culture
Whether upgrowth situation normal, if individual growth situation normally if be transferred to culturing rack continue cultivate, if individual has phenomenon of catching an illness
Or the malnutritive situation of appearance is then transferred to band operating space and is prevented epidemic or topdressed.
The dark treatment room for including being arranged on the detection conveyer belt as preferred, described imaging device, across institute
State the portal frame of detection conveyer belt, and Hyperspectral imager, imaging-PAM light on the portal frame
Spectrometer, thermal infrared camera and 3D cameras.
, it is necessary to carry out the dark treatment of enough time to plant before plant being handled because of imaging-PAM system；It is described
The dark treatment room that detecting system is included can carry out dark treatment in real time on a moving belt, and chlorophyll imaging system carries out work simultaneously
Make, the mode of this parallel work-flow substantially increases detection efficiency.
In the present invention, the problem of EO-1 hyperion system solves " having figure without spectrum, have spectrum without figure ", and there is provided substantial amounts of number
According to；Chlorophyll fluorescence is closely related with plant photosynthesis, can directly reflect photosynthesis of plant speed and stress state；Heat
Infrared imagery technique has very big advantage in disease early diagnosis；3D cameras can effectively obtain the monochrome information of tested sample
And depth information, it is the cutting edge technology for measuring imaging field；Derived techniques means comprehensive, multi-faceted can obtain detection pair
The spectral information of elephant, while mutually comparing, effectively improves detection performance.
Include visible near-infrared 380-1023nm spectrometers and near-infrared as preferred, described Hyperspectral imager
874-1734nm imaging spectrometers, and equipped with two CCD cameras and supporting splitting module and imaging lens.
As preferred, spaced twice automatically-controlled door is set in the dark treatment room, by the dark treatment interior space point
Into treatment region and area to be checked, first of automatically-controlled door is located at the treatment region and interval to be checked, and second automatically-controlled door is located at described treat
Examine between area and imaging device.
In the present invention, second automatically-controlled door is first opened, and the potted plant of area to be checked is transported to detection zone by conveyer belt, then second
Door is closed, first automatically-controlled door open by dark treatment cross it is potted plant be delivered to by detection conveyer belt it is to be checked in the middle of two automatically-controlled doors
Area, can so avoid influence of the illumination of detection zone to dark treatment region.
As between preferred, described detecting system, control system and Transport Machinery arm use wirelessly transmitting data.
System in the present invention, can carry out periodic detection in the full growth period of rape, find to catch an illness and prevented in time
Epidemic disease, loss is reduced, or rape is topdressed in time, is realized precisely plantation, can be reduced breeding interindividual variation, shortens
Cultivation cycle.
The present invention sets up growth of rape high levels of three-dimensional model and leaf color disaggregated model based on EO-1 hyperion and 3D rendering, sets up oil
Vegetable quality detection model and Yield Estimation Model and its visualization inverting, realize that plant height, leaf color difference, Soluble sugar contents, pigment contain
The quick obtaining of the breeding key phenotypic information such as amount and seed oleic acid content and rapeseed production information.Using chlorophyll fluorescence into
As technology gathers the image of different genotype rape variety, fluorescent characteristics parameter is extracted, is later stage high-throughout resistant gene sieve
It is elected to be preparation.Obtain the optimal spectrum wave band and optimal vegetation index of rape each growth period inside quality detection, and oil
The optimum growh of each inside quality detection of dish, finally auxiliary rapeseed breeding easily and effectively, is the height of China's crop breeding information
Flux detection provides important directive significance.
Compared with prior art, the present invention has advantages below and beneficial effect：
The present invention combines culture part in breeding chamber with detection part, and substantial length of operation can automate progress, carry
High space availability ratio and operating efficiency；The periodic detection in full growth period is carried out to rapeseed breeding, dye can be not only found early
Sick phenomenon, loss is reduced, and can also be judged whether to need to topdress to the rape of culture according to testing result, artificial light source
Photosynthesis is needed most during compensation growth of rape feux rouges and blue light, the pattern that this interior is precisely cultivated can shorten oil
Dish is cultivated time 3-5 month, and increase yield at least 20%, is adapted to extensive greenhouse rapeseed breeding.
Brief description of the drawings
Fig. 1 is the high flux phenotype research system diagram of the full growth period information of rape；
Fig. 2 is each imaging system position figure of detecting system；
Fig. 3 is detecting system side view；
Fig. 4 is breeding method flow chart.
Embodiment
Describe the present invention in detail with reference to embodiment and accompanying drawing, but the present invention is not limited to this.
High flux phenotype research system as shown in Figure 1, including rapeseed breeding culture line, Transport Machinery arm 16, detection system
System, control system 2 and frame 15.
Rapeseed breeding culture line, including culturing rack 4, the Culture basin 7 with Electronic Numbering, compensatory light 17 are equipped with artificial photograph
It is bright, LED/light source compensation function, feux rouges and blue light needed for supplement rape photosynthesis；Culturing rack 4 is sequentially placed in culturing room
Interior, Culture basin 7 is located on culturing rack 4.
Transport Machinery arm 16, multiple mechanical arms can coordinate completion is following to operate：Culture basin is moved into detection conveyer belt；According to
The instruction sorting of control system grows normal individual and caught an illness or underfed abnormal individuals, and normal individual is sent back to culture
Frame, abnormal individuals are transferred to pending district.
Detecting system as shown in Figures 2 and 3, including dark treatment room 3, detection conveyer belt 8, Hyperspectral imager 10 have
Visible near-infrared (380-1023nm) and near-infrared (874-1734nm) imaging spectrometer (is furnished with two CCD cameras and supporting point
Optical mode group and imaging lens), imaging-PAM spectrometer 11, thermal infrared camera 12,3D cameras 13, code reader 9 and detection
Portal frame 14；Portal frame 14 is arranged on the intermediate region of detection conveyer belt 8；Each spectrum imaging system and code reader 9 are installed successively
On portal frame 14；Process chamber is divided into treatment region and area to be checked by two automatic door devices 5 in dark treatment room 3, when transmitting potted plant,
Second automatically-controlled door is first opened, and the potted plant of area to be checked is transported to detection zone by conveyer belt, and then second door is closed, and first automatic
Door opens the potted plant area to be checked being delivered to by detection conveyer belt 8 in the middle of two automatically-controlled doors for crossing dark treatment, can so avoid
Influence of the illumination of detection zone to dark treatment region.
In the present embodiment, control system 2 is installed in operation frame 15, carried out with other parts by Wireless Transmitter 1
Communication.
As shown in figure 4, using the high flux phenotype research system of the above-mentioned full growth period information of rape, realizing rapeseed breeding
The step of include：
1) system initialization；
2) Culture basin is transported detection conveyer belt 8 by the Transport Machinery arm 16 on rape culture line, starts control system；
3) control system starts detection conveyer belt, and hydroponic box is managed room by conveyer belt in the dark, and the length of dark treatment room is enough
Reach dark treatment the time required；
4) Culture basin enters area to be checked；
5) Culture basin enters at portal frame detecting system；
6) imaging system detects imaging successively in code reader identification Culture basin electronic identity numbering, detecting system, and will figure
As information by Wireless Transmitter is sent to control system；
7) image information that control system is transmitted to detecting system is analyzed, and judges whether rape has catch an illness phenomenon and length
Gesture, and the corresponding control command that analysis result is drawn is sent to Transport Machinery arm；
6) Culture basin is transported detecting system by detection conveyer belt；
7) instruction that Transport Machinery arm is sent according to control system, corresponding Culture basin is transported back to culturing rack or is transported to
Area to be operated.
The foregoing is only the preferable implementation example of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (6)
1. a kind of high flux phenotype research system of the full growth period information of rape, it is characterised in that including：
Rapeseed breeding culture line, with an at least culturing rack, places the Culture basin with Electronic Numbering, and match somebody with somebody on the culturing rack
There is the LED/light source of compensation illumination；
Detecting system, including detection conveyer belt, for the imaging device of the Culture basin imaging to being conveyed on detection conveyer belt, and
Recognize the code reader of the Electronic Numbering；
Control system, for analyzing image, and judges whether the growth in Culture basin is normal；
Transport Machinery arm, for capturing described Culture basin between rapeseed breeding culture line and detecting system, and according to growth of rape
Situation puts the position of the Culture basin.
2. high flux phenotype research system as claimed in claim 1, it is characterised in that described LED/light source includes red-light LED
Light source and blue light LED light source.
3. high flux phenotype research system as claimed in claim 1, it is characterised in that described imaging device includes being arranged in
Dark treatment room on the detection conveyer belt, across the portal frame of the detection conveyer belt, and on the portal frame
Hyperspectral imager, imaging-PAM spectrometer, thermal infrared camera and 3D cameras.
4. high flux phenotype research system as claimed in claim 3, it is characterised in that described Hyperspectral imager includes
Visible near-infrared 380-1023nm spectrometers and near-infrared 874-1734nm imaging spectrometers, and equipped with two CCD cameras and match somebody with somebody
Cover splitting module and imaging lens.
5. high flux phenotype research system as claimed in claim 1, it is characterised in that set spaced in the dark treatment room
Twice automatically-controlled door, is divided into treatment region and area to be checked by the dark treatment interior space, and first of automatically-controlled door is located at the treatment region
With interval to be checked, second automatically-controlled door is located between the area to be checked and imaging device.
6. high flux phenotype research system as claimed in claim 1, it is characterised in that described detecting system, control system
Wirelessly transmitting data is used between Transport Machinery arm.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107860366A (en) * | 2017-10-31 | 2018-03-30 | 南京农业大学 | A kind of portable chamber crop information measurement and diagnostic system based on multispectral space engraving technology |
| CN108982446A (en) * | 2018-07-06 | 2018-12-11 | 浙江大学 | A kind of inline diagnosis and the imaging-PAM system and method for being classified crop disease |
| CN110089313A (en) * | 2019-04-24 | 2019-08-06 | 浙江大学 | A kind of three-dimensional full-automatic online phenotype high throughput detection platform of crop |
| CN110095918A (en) * | 2019-04-26 | 2019-08-06 | 南京农业大学 | A kind of root system of plant automation imaging system based on flat cultivating container |
| CN112710663A (en) * | 2021-01-17 | 2021-04-27 | 无锡职业技术学院 | High-throughput measurement system and measurement method for phenotype information of plant whole life cycle |
| CN113711805A (en) * | 2021-09-22 | 2021-11-30 | 无锡职业技术学院 | A cultivate room for accurate cultivation of crops |
| CN113728838A (en) * | 2021-09-22 | 2021-12-03 | 无锡职业技术学院 | Accurate prevention and control device of crops based on coercion phenotype property |
| CN113924965A (en) * | 2021-11-15 | 2022-01-14 | 中国农业科学院都市农业研究所 | System and method for industrial plant production |
| CN114080939A (en) * | 2020-08-24 | 2022-02-25 | 立派特科股份有限公司 | Birth information collecting device |
| WO2022060293A1 (en) * | 2020-09-18 | 2022-03-24 | Agency For Science, Technology And Research | Optical system, methods of forming and operating the same |
| CN116907576A (en) * | 2023-07-13 | 2023-10-20 | 广东省农业科学院设施农业研究所 | Automatic seedling detection system, method and electronic equipment |
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