CN107131929A - A kind of self-calibrating method of sample loading gun - Google Patents
A kind of self-calibrating method of sample loading gun Download PDFInfo
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- CN107131929A CN107131929A CN201710211986.0A CN201710211986A CN107131929A CN 107131929 A CN107131929 A CN 107131929A CN 201710211986 A CN201710211986 A CN 201710211986A CN 107131929 A CN107131929 A CN 107131929A
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- sample loading
- loading gun
- calibrated
- sample
- luminous
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F25/00—Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume
- G01F25/0084—Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume for measuring volume
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- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- General Physics & Mathematics (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of self-calibrating method of sample loading gun, comprise the following steps：Benchmark rifle is obtained from inspection center；Luminescent solution is configured, and takes 1 piece of cleaning luminous detection plate；The luminescent solution configured is mixed, and it is luminous to excite to add enzyme conjugates；Benchmark rifle is adjusted to the volume that need to be calibrated to this, added the luminescent solution configured in cleaning luminous detection plate with benchmark rifle, and the top-down continuous sample-adding for several times of same row is carried out to cleaning luminous detection plate；Sample loading gun to be calibrated is adjusted to the volume that need to be calibrated to this, added the luminescent solution configured in cleaning luminous detection plate with sample loading gun to be calibrated, and carry out same row it is top-down it is continuous for several times；Operated more than repeating, read the luminous value of benchmark rifle and sample loading gun to be calibrated；Count the luminous value read and calculate the average value of every sample loading gun, standard deviation SD and coefficient of variation CV；Performance to sample loading gun judges.
Description
Technical field
The present invention relates to a kind of self-calibrating method of sample loading gun, it is adaptable to test in laboratory system.
Background technology
The various equipment of test in laboratory system have been required to corresponding calibration and examined and determine, just can be with after calibration detection is qualified
Come into operation, and be usually directed to the influence of many factors such as reagent, instrument, personnel, environment, existing sample loading gun for sample loading gun
Calibration generally use internal calibration method, i.e., the certain volume drawn with the balance measurement sample loading gun by calibrating is gone
The weight of ionized water, and be corrected with this.But such calibration is to the sample-adding ratio of sample loading gun and examining for continuous sample-adding precision
Examine and still have some deficits, particularly the precision prescribed to surrounding environment is very harsh, under explaining here, by Clapyron Equation PV
=nRT, learns V=nRT/P, thus know the volume of the deionized water of sample loading gun certain volume in other words quality to environment temperature
Requirement it is higher, and detect after sample loading gun can be brought into usually used environment, so may result in calibration after adding
Performance of sample rifle when usually using the performance and calibration showed in environment has gap, so as to cause error.
The content of the invention
It is an object of the invention to provide a kind of self-calibrating method of sample loading gun, for problem of the prior art, in experiment
Carried out in the usually used environment of room, reduce error, it is ensured that the performance of sample loading gun.
In order to realize the above object the technical solution adopted by the present invention is：A kind of self-calibrating method of sample loading gun, including
Following steps：
(1) benchmark rifle is obtained from inspection center；
(2) luminescent solution is configured, and takes 1 piece of cleaning luminous detection plate；
(3) luminescent solution configured is mixed, and it is luminous to excite to add enzyme conjugates；
(4) benchmark rifle is adjusted to the volume that need to be calibrated to this, the luminescent solution configured is added into cleaning hair with benchmark rifle
In light detection plate, and the top-down continuous sample-adding for several times of same row is carried out to cleaning luminous detection plate；
(5) sample loading gun to be calibrated is adjusted to the volume that need to be calibrated to this, will have been configured with sample loading gun to be calibrated
Luminescent solution is added in cleaning luminous detection plate, and the sample-adding is arranged positioned at the adjacent side of step (4), and carries out same row from top to bottom
It is continuous for several times；
(6) operation of repeat step (5), all sample loading guns to be calibrated are loaded by this step, after sample-adding is finished,
Read the luminous value of benchmark rifle and sample loading gun to be calibrated；
(7) count the luminous value read and calculate the average value of every sample loading gun, standard deviation SD and coefficient of variation CV；
(8) performance to sample loading gun judges：If sample loading gun to be calibrated is less than the 10% of benchmark rifle mean deviation,
Then judge that the sample loading gun can be used；, should if the sample loading gun of calibration is more than or equal to the 10% of benchmark rifle mean deviation
The sample loading gun is conserved.
Further, the sample-adding number of times in step (4), step (5), step (6) is consistent.
Further, described sample-adding number of times is 8.
The technical effects of the invention are that：Using the above method, carried out in the usually used environment in laboratory, it is ensured that
The performance of sample loading gun.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
A kind of self-calibrating method of sample loading gun, comprises the following steps：
(1) benchmark rifle is obtained from inspection center；
(2) luminescent solution is configured, and takes 1 piece of cleaning luminous detection plate；
(3) luminescent solution configured is mixed, and it is luminous to excite to add enzyme conjugates；
(4) benchmark rifle is adjusted to the volume that need to be calibrated to this, the luminescent solution configured is added into cleaning hair with benchmark rifle
In light detection plate, and the top-down continuous sample-adding for several times of same row is carried out to cleaning luminous detection plate；
(5) sample loading gun to be calibrated is adjusted to the volume that need to be calibrated to this, will have been configured with sample loading gun to be calibrated
Luminescent solution is added in cleaning luminous detection plate, and the sample-adding is arranged positioned at the adjacent side of step (4), and carries out same row from top to bottom
It is continuous for several times；
(6) operation of repeat step (5), all sample loading guns to be calibrated are loaded by this step, after sample-adding is finished,
Read the luminous value of benchmark rifle and sample loading gun to be calibrated；
(7) count the luminous value read and calculate the average value of every sample loading gun, standard deviation SD and coefficient of variation CV；
(8) performance to sample loading gun judges：If sample loading gun to be calibrated is less than the 10% of benchmark rifle mean deviation,
Then judge that the sample loading gun can be used；, should if the sample loading gun of calibration is more than or equal to the 10% of benchmark rifle mean deviation
The sample loading gun is conserved.
Further, the sample-adding number of times in step (4), step (5), step (6) is consistent.
Further, described sample-adding number of times is 8.
Using the above method, carried out in the usually used environment in laboratory, it is ensured that the performance of sample loading gun.
Described benchmark rifle is that this rifle whole year is without being served only for from the sample loading gun after inspection center annually annual test
The calibration of the inventive method, it is ensured that the accuracy of this method calibration result.Following table is to be compared rifle with 4 groups by benchmark rifle to contrast
The data arrived (volume of this calibration is 50 μ L)：
In upper table, wherein, average value X=(R1+R2+ ...+R8)/8；
SD represents standard deviation, and SD is under radical sign [(X-R1)2+(X-R2)2+…+(X-R8)2]/8；
CV represents the coefficient of variation, CV=[SD (standard deviation)/X (average value)] * 100%；
Mean deviation is the average value X*100% of (the average value X for comparing the basic rifles of average value X- of rifle)/basic rifle；
Using a benchmark rifle in calibration method, remaining rifle is to compare rifle.Wherein performance includes two aspects altogether, and one is
Calibration rifle and the overall extent of deviation for comparing rifle, represent the accuracy of sample-adding；Two be the coefficient of variation after multiple sample-adding, represents to add
The stability of sample.If wherein comparing the average value performance of rifle no more than the 10% of calibration rifle, judgement can be used, if not
Conditions above is met, then is considered as being conserved.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.
Claims (3)
1. a kind of self-calibrating method of sample loading gun, it is characterised in that comprise the following steps：
(1) benchmark rifle is obtained from inspection center；
(2) luminescent solution is configured, and takes 1 piece of cleaning luminous detection plate；
(3) luminescent solution configured is mixed, and it is luminous to excite to add enzyme conjugates；
(4) benchmark rifle is adjusted to the volume that need to be calibrated to this, the luminescent solution configured is added into cleaning luminous inspection with benchmark rifle
On drafting board, and the top-down continuous sample-adding for several times of same row is carried out to cleaning luminous detection plate；
(5) sample loading gun to be calibrated is adjusted to the volume that need to be calibrated to this, it is luminous by what is configured with sample loading gun to be calibrated
Liquid is added in cleaning luminous detection plate, and the sample-adding is arranged positioned at the adjacent side of step (4), and carries out the top-down company of same row
Continue for several times；
(6) operation of repeat step (5), all sample loading guns to be calibrated are loaded by this step, after sample-adding is finished, and are read
The luminous value of benchmark rifle and sample loading gun to be calibrated；
(7) count the luminous value read and calculate the average value of every sample loading gun, standard deviation SD and coefficient of variation CV；
(8) performance to sample loading gun judges：If sample loading gun to be calibrated is less than the 10% of benchmark rifle mean deviation, sentence
The disconnected sample loading gun can be used；If the sample loading gun of calibration is more than or equal to the 10% of benchmark rifle mean deviation, reply should
Sample loading gun is conserved.
2. a kind of self-calibrating method of sample loading gun according to claim 1, it is characterised in that：Step (4), step (5),
Sample-adding number of times in step (6) is consistent.
3. a kind of self-calibrating method of sample loading gun according to claim 2, it is characterised in that：Described sample-adding number of times is
8。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710211986.0A CN107131929A (en) | 2017-04-01 | 2017-04-01 | A kind of self-calibrating method of sample loading gun |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710211986.0A CN107131929A (en) | 2017-04-01 | 2017-04-01 | A kind of self-calibrating method of sample loading gun |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107131929A true CN107131929A (en) | 2017-09-05 |
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ID=59715374
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710211986.0A Pending CN107131929A (en) | 2017-04-01 | 2017-04-01 | A kind of self-calibrating method of sample loading gun |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2033896U (en) * | 1988-06-02 | 1989-03-08 | 吴锡铭 | Digital display correcting instrument for precise pipette |
| US20050196747A1 (en) * | 2004-03-05 | 2005-09-08 | Matthias Stiene | Analyte test system for determining the concentration of an analyte in a physiological or aqueous fluid |
| CN101038202A (en) * | 2007-02-06 | 2007-09-19 | *** | Method for contrasting and correcting accuracy of liquid transfering device volume with europium atom labeling method |
| CN101655423A (en) * | 2009-09-02 | 2010-02-24 | 广州市怡文科技有限公司 | Accurate liquid extracting and metering device and method |
| WO2014008150A1 (en) * | 2012-07-06 | 2014-01-09 | 3M Innovative Properties Company | Apparatus for detecting atp in a liquid sample |
-
2017
- 2017-04-01 CN CN201710211986.0A patent/CN107131929A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2033896U (en) * | 1988-06-02 | 1989-03-08 | 吴锡铭 | Digital display correcting instrument for precise pipette |
| US20050196747A1 (en) * | 2004-03-05 | 2005-09-08 | Matthias Stiene | Analyte test system for determining the concentration of an analyte in a physiological or aqueous fluid |
| CN101038202A (en) * | 2007-02-06 | 2007-09-19 | *** | Method for contrasting and correcting accuracy of liquid transfering device volume with europium atom labeling method |
| CN101655423A (en) * | 2009-09-02 | 2010-02-24 | 广州市怡文科技有限公司 | Accurate liquid extracting and metering device and method |
| WO2014008150A1 (en) * | 2012-07-06 | 2014-01-09 | 3M Innovative Properties Company | Apparatus for detecting atp in a liquid sample |
Non-Patent Citations (1)
| Title |
|---|
| 吕世静，李会强: "《临床免疫学检验（第三版）》", 1 August 2015, 中国医药科技出版社 * |
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Application publication date: 20170905 |
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