CN107129068A - Recovery prepares fish oil, the method for fish protein peptide in a kind of processing waste water from minced fillet - Google Patents
Recovery prepares fish oil, the method for fish protein peptide in a kind of processing waste water from minced fillet Download PDFInfo
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- CN107129068A CN107129068A CN201710300986.8A CN201710300986A CN107129068A CN 107129068 A CN107129068 A CN 107129068A CN 201710300986 A CN201710300986 A CN 201710300986A CN 107129068 A CN107129068 A CN 107129068A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/24—Treatment of water, waste water, or sewage by flotation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/38—Treatment of water, waste water, or sewage by centrifugal separation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/40—Devices for separating or removing fatty or oily substances or similar floating material
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
- C02F1/5245—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents using basic salts, e.g. of aluminium and iron
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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Abstract
The invention provides recovery in a kind of processing waste water from minced fillet prepare fish oil, fish protein peptide method, comprise the following steps：Minced fillet processing waste water is pre-processed；Flocculant, stirring mixing are added into pretreated waste water；Waste water after flocculation is handled using air-float technology, to isolate the organic matter in waste water；Organic matter is digested, centrifuges, obtains fish oil and Fish protein liquid；Fish protein liquid is filtered, then, the flocculant small molecule in Fish protein liquid, concentration are removed using ion exchange resin or ultrafiltration system；Spraying drying powder-forming, obtains fish protein peptide.This method can efficiently recovery prepares fish oil, fish protein peptide both qualified products from minced fillet processing waste water, the COD of the minced fillet processing waste water is caused to drop to 2000~3000mg/L from 16000~30000mg/L simultaneously, solve minced fillet processing waste water easily block pipeline and can not long distance delivery problem, turn waste into wealth simultaneously, with significant economic benefit；The processing procedure of this method is quick and cost of investment is relatively low.
Description
Technical field
The present invention relates to aquatic products processing technical field, and in particular to one kind from minced fillet processing waste water recovery prepare fish oil,
The method of fish protein peptide.
Background technology
Minced fillet processing industry is a kind of important processing of aquatic products industry of China's Coastal Areas, the industry with a variety of fish,
Particularly ocean fish is that raw material carries out minced fillet processing, and the strong albumen of the gelation after its extraction is slaughtered in the flesh of fish is prepared into minced fillet, its
The albumen of remaining great amount of soluble all enters water body in process, forms a kind of high heavily contaminated water quality of content of organic matter.
COD in minced fillet processing waste water is generally in more than 12000mg/L, considerably beyond sanitary sewage COD500mg/L level, to dirt
Cause greatly processing pressure in water treatment plant.If the organic principle in waste water can be extracted, it can not only drop significantly
Intractability in low environmental protection, while can also twice laid, increase economic benefit.In the Protein Extraction skill of wastewater from aquatic product process
Art field, domestic prior art mainly includes：
Chinese patent application CN201510199249.4 discloses one kind and utilizes aluminium polychloride and Sodium Polyacrylate from water
Produce flocculation sediment albumen, the method for being then directly dried into feed in processing waste water.However, this method ignores aluminium polychloride
Toxic action with Sodium Polyacrylate to animal, brings to feed safety and food security and greatly endangers, therefore, to the mankind's
There is sizable hidden danger in safety and health.
Chinese patent application CN201310301925.5 discloses a kind of method for producing fish protein and production fish
The method of protein peptides, this method is removed after degreasing using so-called oil separation equipment, then with the further protein isolate of centrifuge and grease,
Isolated protein solution is concentrated by film, after protease hydrolyzed drying obtain protein peptides.However, found by analysis, should
Method ignores the oil and albumen in minced fillet waste water with the presence of reference state form, and therefore, simple centrifugation can not protein isolate
And fish oil, and the grease remained in albumen easily gambles film, so that whole flow process can not be smoothed out；Especially, largely
Waste water by supercentrifuge, cost of investment is high, energy consumption is big, equipment cleaning and maintenance difficulties are very big.
It is well known that the content of organic matter in minced fillet processing waste water is high, general COD exists in more than 12000mg/L, COD
It is most commonly seen between 16000-30000mg/L；Moreover, the water yield of minced fillet manufacturer is big, water outlet fast, it is necessary to whole treated
Journey is completed in very short time.Therefore, how to develop one kind online quick reclaim can prepare fish from minced fillet processing waste water
Oil, the method for fish protein peptide, are one of research emphasis and difficult point of this area scientific research personnel.
The content of the invention
In order to overcome many disadvantages present in prior art, the present invention is intended to provide one kind can be from minced fillet processing waste water
In online quick recovery prepare fish oil, the method for fish protein peptide；The technique that inventor designs and implemented, it is useless from minced fillet processing first
Reclaim organic matter in water, then organic matter by the method for biology enzyme is prepared into fish oil and fish protein peptide, the technique has been shown
Machine matter reclaims the thorough, technical characterstic that effluent quality is good, processing procedure is quick, investment is small, income is high.
Therefore, the invention provides recovery in a kind of processing waste water from minced fillet prepare fish oil, fish protein peptide method, specifically
Comprise the following steps：
S1：Minced fillet processing waste water is pre-processed using hydraulic screen or centrifuge, to remove large volume impurity；Wherein,
Described large volume impurity is, for example, fish head, fish-bone, internal organ and fin etc.；
S2：Flocculant, stirring mixing are added into pretreated waste water；
S3：Waste water after flocculation is handled using air-float technology, to isolate the organic matter in waste water；
S4：The organic matter isolated in enzymolysis S3, is then centrifuged for separation, obtains fish oil and Fish protein liquid；
S5：By Fish protein liquid filtering, then, the Fish protein is removed using ion exchange resin or ultrafiltration system
Flocculant small molecule in liquid, concentration；
S6：Spraying drying powder-forming, obtains fish protein peptide.
Optionally, in addition, in step sl, while removing large volume impurity in waste water, scrapper conveyor also can use to collect useless
The fish oil of surface layer of water.
Preferably, the filtering described in the S5 of the above method is implemented using bag filter or microfiltration membranes.
Preferably, the flocculant described in the S2 of the above method is selected from following any one or more combination：It polymerize chlorine
Change aluminium, poly-ferric chloride, polyacrylamide.
Wherein, in the S3 of the above method, the organic matter (containing fish oil etc.) on the upper strata isolated is collected with scrapper conveyor, and
The organic matter for being deposited to bottom then uses sludge pumped.
Preferably, in the S4 of the above method, before implementing to digest：(1) organic matter isolated first is removed into bubble；Or
It is 20~50wt% that the organic matter isolated first is dehydrated to water content by person (2).
Preferably, in the S4 of the above method, the organic matter isolated in enzymolysis S3 at 40~60 DEG C.
It is further preferred that in the S4 of the above method, first adding 1~50ppm EDTA, then it is warming up to 40~60 DEG C of realities
Apply enzymolysis.
Preferably, in the S4 of the above method, implement to digest using the compound protease of solid content 0.1~0.8%.
It is further preferred that in the above-mentioned methods, the compound protease be selected from it is following it is any two or more
Combination：Pepsin, trypsase, papain, bromelain, alkali protease, Validase TSP Concentrate II, wind
Taste protease, aminopeptidase, carboxypeptidase.
Preferably, in the S5 of the above method, implement to concentrate using nanofiltration system and/or vacuum concentration equipment.
It is further preferred that in the S5 of the above method, the solid content of the Fish protein liquid is concentrated into 30~50%.
Relative to prior art, the technical advantage that technical scheme provided by the present invention possesses includes：
First, method provided by the present invention can efficiently recovery prepares fish oil, Fish protein from minced fillet processing waste water
Both qualified products of peptide, while so that the COD of the minced fillet processing waste water drop to 2000 from 16000~30000mg/L~
3000mg/L；Second, methods described significantly reduces minced fillet processing waste water concentration, solves minced fillet processing waste water and easily blocks pipe
Road and can not long distance delivery problem, turn waste into wealth simultaneously, with significant economic benefit；3rd, methods described causes have
The recovery of machine matter is thorough, effluent quality is good, is easy to follow-up further processing to discharge；The processing procedure of methods described is quick and invests
Cost is relatively low；4th, the fish protein peptide reclaimed according to method provided by the present invention is free of any harmful metal ion, product
It is safe and reliable.
Embodiment
Technical solution of the present invention is further elaborated on reference to embodiment.
Recovery prepares fish oil, the method for fish protein peptide in a kind of processing waste water from minced fillet, comprises the following steps：Use waterpower
Sieve or centrifuge are pre-processed to minced fillet processing waste water, to remove large volume impurity；Then, add into pretreated waste water
Enter flocculant, stirring mixing；Then, the waste water after flocculation is handled using air-float technology, to isolate the organic matter in waste water；Enzyme
The organic matter that solution is isolated, is then centrifuged for separation, obtains fish oil and Fish protein liquid；By the Fish protein liquid filtering, reuse from
Sub-exchange resin or ultrafiltration system remove the flocculant small molecule in the Fish protein liquid, concentration；Finally, it is spray dried to
Powder, obtains fish protein peptide.
In a preferred embodiment, described filtering is implemented using bag filter or microfiltration membranes.
In a preferred embodiment, described flocculant is selected from following any one or more combination：Aluminium polychloride,
Poly-ferric chloride, polyacrylamide.
In a preferred embodiment, before implementing to digest：(1) organic matter isolated first is removed into bubble；Or (2)
It is 20~50wt% that first the organic matter isolated, which is dehydrated to water content,.
In a preferred embodiment, in digesting the organic matter isolated at 40~60 DEG C.
In a further preferred embodiment, 1~50ppm EDTA is first added, then is warming up to 40~60 DEG C of implementations
Enzymolysis.
In a preferred embodiment, implement to digest using the compound protease of solid content 0.1~0.8%.
In a further preferred embodiment, the compound protease be selected from it is following it is any two or more
Combination：Pepsin, trypsase, papain, bromelain, alkali protease, Validase TSP Concentrate II, wind
Taste protease, aminopeptidase, carboxypeptidase.
In a preferred embodiment, implement to concentrate using nanofiltration system and/or vacuum concentration equipment.
In a further preferred embodiment, implemented using nanofiltration system and/or vacuum concentration equipment by the fish
The solid content of protein liquid is concentrated into 30~50%.
Embodiment 1
According to following steps, recovery prepares fish oil and fish protein peptide from minced fillet processing waste water：
S1：Minced fillet processing waste water is pre-processed using hydraulic screen, the large volumes such as fish head, fish intestines, fish-bone, fin are removed
Impurity, is then kept in waste water input buffer pool, while being collected the fish oil on waste water top layer using scrapper conveyor；
S2：Lower floor's water of buffer pool flows into stirring pool, adds flocculant, stirring mixing；Wherein, flocculant concentration order
First to add aluminium polychloride, again addition polymerization acrylamide；Wherein, the concentration that adds of aluminium polychloride is 100~300ppm, polypropylene
The concentration that adds of acid amides is 30-100ppm；
S3：Waste water after flocculation is handled using air-float technology, the organic matter in waste water is isolated；By the upper strata isolated
Organic matter is collected with scrapper conveyor, and the organic matter for being deposited to bottom then uses sludge pumped；Wherein, after air-float technology is handled
Water outlet COD in 2800mg/L or so；
S4：The organic matter isolated is pooled together, is slowly stirred, bubble is removed；Solid content is adjusted to 30%, then
30ppm EDTA is added, 40~60 DEG C are then warming up to, compound protease (papain and the wind of solid content 0.3% is added
Taste protease) digest 3 hours；After enzymolysis terminates, material temperature is increased to 85~95 DEG C, is incubated 15~40 minutes enzymes that go out；Then, digest
Liquid is separated by centrifuge, removes insoluble matter, and supernatant is separated by disc centrifuge, obtains fish oil and fish egg
White liquor；
S5：The Fish protein liquid is filtered using bag filter, then, the Fish protein is removed using ion exchange resin
Iron ion and chlorion in liquid etc. and flocculant small molecule, are concentrated using nanofiltration system；
S6：Further use after multi-effect evaporator is concentrated into solid content for 30~50%, spraying drying powder-forming is final to obtain
Fish protein Gly-His-Lys；After testing, the Fish protein Gly-His-Lys obtained are included：Crude protein>65wt%, crude fat<0.5wt%, small peptide>
23wt%, moisture<8wt%.
Embodiment 2
According to following steps, recovery prepares fish oil and fish protein peptide from minced fillet processing waste water：
S1：Minced fillet processing waste water is pre-processed using hydraulic screen, the large volumes such as fish head, fish intestines, fish-bone, fin are removed
Impurity, is then kept in waste water input buffer pool, while being collected the fish oil on waste water top layer using scrapper conveyor；
S2：Lower floor's water of buffer pool flows into stirring pool, adds flocculant, stirring mixing；Wherein, flocculant concentration order
First to add poly-ferric chloride, again addition polymerization acrylamide；Wherein, the concentration that adds of poly-ferric chloride is 100~300ppm, polypropylene
The concentration that adds of acid amides is 30-100ppm；
S3：Waste water after flocculation is handled using air-float technology, the organic matter in waste water is isolated；By the upper strata isolated
Organic matter is collected with scrapper conveyor, and the organic matter for being deposited to bottom then uses sludge pumped；Wherein, after air-float technology is handled
Water outlet COD in 2600mg/L or so；
S4：The organic matter isolated is pooled together, the organic matter isolated is dehydrated using disk screw extruder
It is 35wt% to water content；Then solid content is adjusted to 25%, is then warming up to 40~60 DEG C, add solid content 0.6%
Compound protease (Validase TSP Concentrate II and aminopeptidase) is digested 2.5 hours；After enzymolysis terminates, material temperature is increased to 85~95
DEG C, it is incubated 15~40 minutes enzymes that go out；Then, enzymolysis liquid is separated by centrifuge, removes insoluble matter, and supernatant is passed through
Disc centrifuge is separated, and obtains fish oil and Fish protein liquid；
S5：The Fish protein liquid is filtered using bag filter, then, is removed using ultrafiltration system in the Fish protein liquid
Flocculant small molecule, then using nanofiltration system concentration；
S6：Further use after multi-effect evaporator is concentrated into solid content for 30~50%, spraying drying powder-forming is final to obtain
Fish protein Gly-His-Lys；After testing, the Fish protein Gly-His-Lys obtained are included：Crude protein>56wt%, crude fat<0.8wt%, small peptide>
30wt%, moisture<10wt%.
The specific embodiment of the present invention is described in detail above, but it is intended only as example, and the present invention is not limited
It is formed on particular embodiments described above.To those skilled in the art, it is any to the equivalent modifications that carry out of the present invention and
Substitute also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. recovery prepares fish oil, the method for fish protein peptide in a kind of processing waste water from minced fillet, it is characterised in that including following step
Suddenly：
S1：Minced fillet processing waste water is pre-processed using hydraulic screen or centrifuge, to remove large volume impurity；
S2：Flocculant, stirring mixing are added into pretreated waste water；
S3：Waste water after flocculation is handled using air-float technology, to isolate the organic matter in waste water；
S4：The organic matter isolated in enzymolysis S3, is then centrifuged for separation, obtains fish oil and Fish protein liquid；
S5：By Fish protein liquid filtering, then, removed using ion exchange resin or ultrafiltration system in the Fish protein liquid
Flocculant small molecule, concentration；
S6：Spraying drying powder-forming, obtains fish protein peptide.
2. according to the method described in claim 1, it is characterised in that the filtering described in S5 uses bag filter or microfiltration membranes
Implement.
3. according to the method described in claim 1, it is characterised in that the flocculant described in S2 is selected from following any one or more
Combination：Aluminium polychloride, poly-ferric chloride, polyacrylamide.
4. before according to the method described in claim 1, it is characterised in that in S4, implementing enzymolysis：(1) it will first isolate
Organic matter removes bubble；Or it is 20~50wt% that the organic matter isolated first is dehydrated to water content by (2).
5. according to the method described in claim 1, it is characterised in that in S4, isolated in enzymolysis S3 at 40~60 DEG C
Organic matter.
6. method according to claim 5, it is characterised in that in S4, first adds 1~50ppm EDTA, then is warming up to
40~60 DEG C are implemented enzymolysis.
7. according to the method described in claim 1, it is characterised in that in S4, using the compound egg of solid content 0.1~0.8%
White enzyme implements enzymolysis.
8. method according to claim 7, it is characterised in that the compound protease is selected from following any two kinds or two kinds
Combination above：Pepsin, trypsase, papain, bromelain, alkali protease, bacillus subtilis egg
White enzyme, flavor protease, aminopeptidase, carboxypeptidase.
9. according to the method described in claim 1, it is characterised in that in S5, using nanofiltration system and/or vacuum concentration equipment
Implement concentration.
10. method according to claim 9, it is characterised in that in S5, the solid content of the Fish protein liquid is concentrated into
30~50%.
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Cited By (4)
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| CN111808904A (en) * | 2020-07-23 | 2020-10-23 | 临沂山松生物制品有限公司 | Preparation method for extracting hydrolyzed protein from whey waste liquid |
| CN112456713A (en) * | 2020-11-24 | 2021-03-09 | 广东闻扬环境科技有限公司 | Process and equipment for recovering and treating protein in bean product wastewater |
| CN112715743A (en) * | 2019-10-14 | 2021-04-30 | 湖北工业大学 | Method for recovering protein and fish oil in minced fillet rinsing water and reducing COD (chemical oxygen demand) |
| CN115449534A (en) * | 2022-10-09 | 2022-12-09 | 浙江大学舟山海洋研究中心 | Mixed enzymolysis method of gulfweed and minced fillet wastewater |
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| CN112715743A (en) * | 2019-10-14 | 2021-04-30 | 湖北工业大学 | Method for recovering protein and fish oil in minced fillet rinsing water and reducing COD (chemical oxygen demand) |
| CN112715743B (en) * | 2019-10-14 | 2022-06-10 | 湖北工业大学 | Method for recovering protein and fish oil in minced fillet rinsing water and reducing COD (chemical oxygen demand) |
| CN111808904A (en) * | 2020-07-23 | 2020-10-23 | 临沂山松生物制品有限公司 | Preparation method for extracting hydrolyzed protein from whey waste liquid |
| CN111808904B (en) * | 2020-07-23 | 2023-03-31 | 临沂山松生物制品有限公司 | Preparation method for extracting hydrolyzed protein from whey waste liquid |
| CN112456713A (en) * | 2020-11-24 | 2021-03-09 | 广东闻扬环境科技有限公司 | Process and equipment for recovering and treating protein in bean product wastewater |
| CN115449534A (en) * | 2022-10-09 | 2022-12-09 | 浙江大学舟山海洋研究中心 | Mixed enzymolysis method of gulfweed and minced fillet wastewater |
| CN115449534B (en) * | 2022-10-09 | 2023-05-16 | 浙江大学舟山海洋研究中心 | Mixed enzymolysis method for gulfweed and minced fillet wastewater |
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